JP2020186058A - Container, container with product therein, production method for container with product therein, and thawing method and production method for cell preparation - Google Patents

Abstract

To provide a container preventing attachment of liquid on an open part of the container.SOLUTION: A container 10 containing biological material comprises: a container body 20 comprising film material 23 having a first surface and a second surface facing each other, at least a part of the film material 23 where the first surface and the second surface face each other being connected, and a first end part 241 and a second end part 242 opposite to the first end part in a first direction; and a widened part 40 connected to a connection part 29 at a position between the first end part 241 and the second end part 242 in the first direction of the container body 20, wherein the container body 20 comprises a container part 21 positioned at a position nearer to the first end part 241 than an end part of the first end part 29b of the connection part 29 in the first direction to contain product to be contained, and the second end part 242 is sealed with a connection part 22 and a first connection part has a seal strength of 3 N/15 mm or more to 6 N/15 mm or less at least in part.SELECTED DRAWING: Figure 1

Description

The present disclosure relates to a container, a container containing an inclusion, a method for producing a container containing an inclusion, a thawing method, and a method for producing a cell preparation.

For example, as disclosed in Patent Document 1 and Patent Document 2, containers for storing frozen contents are known. The container is used as a container for a biological substance for storing a biological substance such as a cell or a substance secreted from the cell in a frozen state. The container for biological material is particularly used as a container for cells, in which cultured cells are stored in a frozen state until use. Immediately before use, the contents in the container are thawed by heat exchange with a moderately heated liquid together with the container. For example, when a container contains a container containing a biological substance such as a cell or a secretory substance, the container can be contained by immersing the container in a liquid such as water maintained at 30 ° C to 40 ° C. It is unzipped.

Japanese Unexamined Patent Publication No. 2018-110539 Japanese Unexamined Patent Publication No. 2001-70402

For example, when thawing the contents in the container, it may be required to immerse a part of the container in a liquid. In this case, the contents may be contaminated by the liquid adhering to the opening for containing the contents in the container or the opening for removing the contents from the container.

The present disclosure has been made in consideration of such a point, and an object of the present disclosure is to effectively suppress the liquid from adhering to the opening of the container.

The container according to this disclosure is
A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
The second end portion is closed by a joint portion formed by joining the film materials.
The container body has a start portion that includes a notch on the second end side of the connection portion in the first direction and serves as a starting position for cutting the container body, or
Of the joints, the first joint located on the second end side of the connection in the first direction has at least a partial sealing strength of 3N / 15 mm or more and 6N / 15 mm or less.

Another container according to this disclosure is
A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
Further, a tubular member connected to the second end side of the container body so as to communicate with the storage portion is further provided.
Of the ends of the tubular member, the outer end located outside the container body is closed.

Another container according to this disclosure is
A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
The container body is further provided with a first fitting portion and a second fitting portion which are provided on the inner surface of the film material and can be fitted to each other on the second end side of the connecting portion in the first direction. Have and
The second end portion is closed by fitting the first fitting portion and the second fitting portion.

Another container according to this disclosure is
A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
The accommodating portion communicates with the outside of the container body via a portion of the container body located closer to the second end portion than the connecting portion in the first direction.

The container according to the present disclosure further includes a tubular member connected to the second end side of the container body so as to communicate with the storage portion.
Of the ends of the tubular member, the outer end located outside the container body may be open.

In the container according to the present disclosure, the container body is formed on the second end side of the connection portion in the first direction between the film materials facing each other, and the storage portion is placed outside the container body. Has an opening to let through
The container body may have a start portion that includes a notch and serves as a starting position for cutting the container body on the second end side of the connection portion in the first direction.

In the container according to the present disclosure, the container body is formed on the second end side of the connection portion in the first direction between the film materials facing each other, and the storage portion is placed outside the container body. Has an opening to let through
When the film material is joined in the opening, the opening joint formed by joining the film material in the opening may have a sealing strength of 3N / 15 mm or more and 6N / 15 mm or less.

In the container according to the present disclosure, the container body is provided on the inner surface of the film material on the side of the second end portion of the connecting portion in the first direction, and the first fitting portion and the first fitting portion that can be fitted to each other. It also has two fittings
The accommodating portion may be communicated to the outside of the container body via the first fitting portion and the second fitting portion.

In the container according to the present disclosure, the storage portion has a smaller size in the second direction perpendicular to the first direction than the portion of the container body on the side of the second end portion from the connection portion. May have.

In the container according to the present disclosure, the dimension of the accommodating portion in the first direction may be larger than the dimension of the accommodating portion in the second direction perpendicular to the first direction.

In the container according to the present disclosure, the thickness of the film material in the container body of the container body may be 1 mm or less.

In the container according to the present disclosure, the thermal conductivity of the film material in the container body of the container body may be 0.1 W / m · K or more.

In the container according to the present disclosure, the spreading portion may protrude so as to be separated from a portion other than the connecting portion of the container body when the container is immersed in a liquid from the first end side of the container body. Good.

In the container according to the present disclosure, the volume of the spread portion is V ₁ , the total volume of the volume of the container body including the inside of the portion where the connection portion is located in the first direction and the volume of the accommodating portion. Is V ₂ , the mass of the container is W ₁ , and the density of water is ρ _W , the following equation (1) may be established.

In the container according to the present disclosure, the ratio X (%) represented by the following formula (2) may be 0.3% or more.

In the container according to the present disclosure, the value obtained by dividing the volume of the spread portion by the area of the connecting surface forming the boundary between the spread portion and the connection portion is 0.15 (mm ³ / mm ² ) or more. May be good.

In the container according to the present disclosure, the spread portion is a spread main body portion that spreads in a direction intersecting the first direction and a spread main body when the container is immersed in a liquid from the first end side of the container body. It may have a spreading auxiliary portion connected to an end portion of the portion on a side away from the container body.

In the container according to the present disclosure, the volume of the spread portion is V ₁ , the volume of the container body including the inside of the portion where the connection portion is located in the first direction, and the volume of the accommodating portion including the inside. When the total volume of the above is V ₂ and the volume of the spreading auxiliary portion is V ₃ , the ratio Y (%) represented by the following formula (3) may be 0.2% or more.

In the container according to the present disclosure, the spread portion may have symmetry when observed from the first direction.

In the container according to the present disclosure, the spread portion may have a bag-shaped portion containing contents that expand when heated.

In the container according to the present disclosure, the bag-shaped portion may be in an upright state toward a side away from the container body by warming and expanding the contents.

The container according to the present disclosure may further include a lid material that detachably closes the outer end portion.

In the container according to the present disclosure, the tubular member may have a thin-walled portion that closes the outer end portion.

In the container according to the present disclosure, the outer end portion may be closed by a sealing portion formed by joining the facing side surfaces of the tubular member.

The container containing the container according to the present disclosure is a container containing the container including the above-mentioned container and the frozen container located inside the container.
Of the frozen container located inside the container, 50% by mass or more is contained in the container.

The method for manufacturing a container containing an container according to the present disclosure includes a supply step of supplying the container to the container via the tubular member of the container described above.
A sealing step for sealing the outer end portion is provided.

Another method of manufacturing a container containing a container according to the present disclosure includes a supply step of supplying the container to the container through the opening of the container described above.
A sealing step for sealing the opening is provided.

The thawing method according to the present disclosure is a thawing method in which a frozen container contained in the container of the above-mentioned container is thawed by heat exchange with a liquid.
The container is provided with a thawing step of immersing the container in the liquid from the first end side of the container body.

In the thawing method according to the present disclosure, the volume of the spread portion is V ₁ , the volume of the container body including the inside of the portion where the connection portion is located in the first direction, and the volume of the storage portion including the inside. The following equation (1) may be established when the total volume of the above is V ₂ , the mass of the container is W ₁ , and the density of the liquid is ρ.

The method for producing a cell preparation according to the present disclosure is a method for producing a cell preparation, wherein the cell preparation is manufactured by thawing frozen cells housed in the container of the above-mentioned container by heat exchange with a liquid. ,
The container is provided with a thawing step of immersing the container in the liquid from the first end side of the container body.

According to the present disclosure, it is possible to effectively prevent the liquid from adhering to the opening of the container.

FIG. 1 is a diagram for explaining an embodiment of the present disclosure, and is a perspective view showing a specific example of a container. FIG. 2 is a front view showing the container of FIG. FIG. 3 is a side view showing the container of FIG. FIG. 4 is a view for explaining the operation of the container of FIG. 1, and is a side view showing the container. FIG. 5 is a perspective view showing a container before the contents are contained therein. FIG. 6 is a front view showing the container of FIG. FIG. 7 is a cross-sectional view showing the container of FIG. FIG. 8 is a diagram showing a state in which a general container having a cylindrical shape is floated on a liquid. FIG. 9 is a diagram showing a state in which a general container having a cylindrical shape is floated on a liquid. FIG. 10 is a view for explaining the operation of the container of FIG. 1, and is a side view showing the container. FIG. 11 is a view corresponding to FIG. 1 and is a perspective view showing another specific example of the container. FIG. 12 is a side view showing the container of FIG. FIG. 13 is a view for explaining the operation of the container of FIG. 11, and is a side view showing the container. FIG. 14 is a view corresponding to FIG. 2 and is a front view showing still another specific example of the container. FIG. 15 is a view for explaining the operation of the container of FIG. 14, and is a side view showing the container. FIG. 16 is a perspective view showing the container of FIG. FIG. 17 is a view corresponding to FIG. 4, and is a side view for explaining still another specific example of the container and its operation. FIG. 18 is a cross-sectional view showing the container of FIG. FIG. 19 is a view corresponding to FIG. 1 and is a perspective view showing still another specific example of the container. FIG. 20 is a view for explaining the operation of the container of FIG. 19, and is a side view showing the container. FIG. 21 is a view corresponding to FIG. 1 and is a perspective view showing still another specific example of the container. FIG. 22 is a view corresponding to FIG. 1 and is a perspective view showing still another specific example of the container. FIG. 23 is a view corresponding to FIG. 1 and is a perspective view showing still another specific example of the container. FIG. 24 is a partial cross-sectional view showing still another specific example of the container. FIG. 25 is a partial cross-sectional view showing still another specific example of the container. FIG. 26 is a view corresponding to FIG. 2 and is a side view showing still another specific example of the container. FIG. 27 is a view corresponding to FIG. 2 and is a side view showing still another specific example of the container. FIG. 28 is a view corresponding to FIG. 1 and is a perspective view showing still another specific example of the container. FIG. 29 is a view for explaining the operation of the container of FIG. 28, and is a side view showing the container.

An embodiment of the present disclosure will be described with reference to the drawings. In the drawings attached to the present specification, the scale, aspect ratio, etc. are appropriately changed from those of the actual product and exaggerated for the convenience of illustration and comprehension.

1 to 4 are diagrams for explaining one embodiment of the present disclosure, and show a specific example of a container.

The container 5 containing the container has a container 10 and a container C housed in the container 10. The container 10 contains the container C. In the present embodiment, as an example, the container 10 used when thawing the frozen container C by heat exchange with the medium 51 described later will be described. The medium 51 is, for example, a liquid 511. Further, the inclusion C is, for example, a biological substance. The biological substance is a substance that can exist in the body of an organism, and examples thereof include cells and secretory substances secreted from cells. As a specific example, the container C contained in the container 10 includes a tissue such as a human body, cultured cells such as a cell sheet, and a cell suspension containing cells before culturing. Generally, a container C containing a biological substance such as a cell is stored in a frozen state until use. Immediately before use, the container C in the container 10 is immersed in a medium 51 that has been appropriately heated together with the container 10. The container C containing the biological material thawed by heat exchange with the medium 51 is taken out from the container 10 through the take-out opening. In particular, the container 10 according to the present embodiment is devised to effectively prevent the medium 51 from adhering to the take-out opening during the thawing of the container C using the medium 51. By preventing the medium 51 from adhering to the take-out opening, it is possible to effectively avoid contamination of the contained material C taken out through the take-out opening.

First, one embodiment will be described with reference to one specific example shown in FIGS. 1 to 4. 1 to 3 are a perspective view, a front view, or a side view showing a container 10 containing a biological substance. FIG. 4 is a diagram showing a state when the container 10 is floated on the medium 51.

As shown in FIGS. 1 to 3, the container 10 has a container body 20 and a floating member 401 connected to the container body 20. Here, the floating member 401 corresponds to a spreading portion 40, which will be described later, formed of a material having a specific gravity smaller than that of the medium 51 used for thawing the contained material C or the like. That is, the floating member 401 is a concept included in the expanding portion 40, and it can be said that the container 10 has the expanding portion 40 by having the floating member 401. Hereinafter, the portion of the container body 20 connected to the spreading portion 40 is also referred to as a connecting portion 29. Further, the container body 20 has a storage unit 21 for storing the container C. The container body 20 preferably has a substantially flat shape so as to promote heat exchange between the medium 51 and the container C. The container body 20 is a film material 23 having a first surface 23d and a second surface 23e facing each other, and the film material 23 to which at least a part of a portion where the first surface 23d and the second surface 23e face each other is joined. Has. As an example, the surface of the film material 23 is flat. In one example, the container body 20 has two resin film materials 23 that are overlapped and the edges are joined, or one resin film material 23 that is folded back and the edges are joined. ing. When the container body 20 has two film materials 23 in which the edges are joined to each other, one of the two film materials 23 constitutes the first surface 23d and the other constitutes the second surface 23e. To do. Further, when the container body 20 has a single film material 23 that is folded back and the edges are joined, the first surface 23d and the second surface 23e are a part of the one film material 23, and the film material 23 Face each other by being folded back. As another example, the container body 20 may have a tubular film material 23 to which the edges are joined. In the example shown in FIG. 2, the container body 20 has a rectangular shape in a plan view. The storage space inside the storage portion 21 of the container body 20 is surrounded by the joint portion 22 from all sides. The joint portion 22 is formed, for example, by heat-welding the facing portions of the resin film material 23. That is, the container body 20 can be manufactured by superimposing two resin film materials 23 and heat-welding the four peripheral edges. The container body 20 can also be manufactured by folding back a single resin film material 23 and heat-welding the three edges. Further, the container body 20 can also be manufactured by closing the two openings of the tubular film material obtained by the inflation bag making by heat welding. By this joint portion 22, the accommodating portion 21 can be kept in a closed state, and the aseptic state of the accommodating portion 21 can be maintained. However, the joining portion 22 is not limited to this example, and may include a joining layer such as an adhesive or an adhesive that joins the facing portions of the film material 23.

In the example shown in FIGS. 1 to 4, the container body 20 forms an outlet opening when the container C is taken out from the container body 20. The take-out opening connects the container 21 of the container body 20 to the outside of the container 10 when the container C is taken out from the container body 20. The take-out opening is formed, for example, by peeling off the facing portions of the film material 23 from each other in the easy joining portion 22a formed of a part of the joining portion 22. Compared with other parts of the joint portion 22, the easy joint portion 22a lowers the temperature during the heat welding process for forming the joint portion 22, shortens the processing time, lowers the processing pressure, or lowers the processing pressure. It is obtained by a combination of two or more selected from these. Hereinafter, the portion where the take-out opening is planned to be formed is also referred to as the take-out opening planned portion 16.

As another example of the take-out opening, the take-out opening may be formed by cutting the container body 20 along the alternate long and short dash line in FIG. In such an example, as shown by the alternate long and short dash line in FIG. 2, the container body 20 may have a start portion 26 having a configuration that serves as a cutting start position. The start portion 26 may be configured as a slit or a notch formed in the film material 23 forming the joint portion 22. Further, as shown by the dotted line in FIG. 2, the container body 20 may have a cutting portion 27 having a structure that facilitates cutting of the film material 23. The cut portion 27 can be formed by a thin portion provided in the film material 23 that extends linearly, or a linear or linear notch or notch that does not penetrate the film material 23.

The container body 20 needs to have resistance to contact with the medium 51 during thawing of the container C. More specifically, it is preferable that the container body 20 has physical properties that can be physically withstood in an environment of at least 30 ° C. or higher, typically in an environment of 37 ° C. or higher. The container body 20 preferably has physical properties that can withstand physically at 60 ° C. or higher, particularly preferably at 100 ° C. or higher, which is the boiling point of water as a typical medium 51. Further, the container body 20 is preferably used for the freezing treatment of the contained material C. That is, it is preferable that the container body 20 has resistance to both high temperature during thawing and low temperature during freezing. More specifically, the temperature of the container body 20 is at least 0 ° C. or lower, preferably −80 ° C. or lower, more preferably −150 ° C. or lower under a liquid nitrogen gas phase, and particularly preferably -196 ° C. or lower which can maintain nitrogen in a liquid state. It is preferable that the material has physical properties that can be physically tolerated in the above environment. Here, physically tolerable means that the film material 23 constituting the container body 20 is not damaged or the film material 23 is not peeled off at the joint portion 22. Further, being physically tolerable may mean that the container body 20 is not damaged, the airtightness of the container 10 is impaired, and the aseptic state inside the container 10 cannot be maintained. Materials used for the container body 20 include, for example, low density polyethylene, linear low density polyethylene, medium density polyethylene, high density polyethylene, ultrahigh molecular weight polyethylene, nylon, polyester, polystyrene, ethylene-vinyl acetate copolymer, fluorine. System resins, vinyl chloride copolymers, ethylene-methyl methacrylate copolymers, ionomers, polyurethanes, polypropylenes, polystyrenes, polyacetals, phenolic resins, urea resins, epoxy resins, ABS resins, polyethylene terephthalates and the like can be used. The illustrated container body 20 can be manufactured by using a resin film material 23 made of these materials, or by using a resin film material 23 in which a plurality of layers made of these materials are laminated.

The container body 20 will be further described. In the example shown in FIGS. 1 to 4, the container body 20 has a first end portion 241 and a second end portion 242 facing the first end portion 241 in the first direction d1. At least the first end 241 is closed. As described above, in the example shown in FIG. 2, the container body 20 extends in the first direction d1 and has a rectangular shape in a plan view. The container body 20 extends in the second direction d2 intersecting the first direction d1 and extends in the first direction d1 and faces the first side 20a and the second side 20b facing each other in the first direction d1. It has a third side 20c and a fourth side 20d. In the example shown in FIG. 2, the second direction d2 is perpendicular to the first direction d1. The first end portion 241 is formed by the first side 20a, and the second end portion 242 is formed by the second side 20b.

Further, the connecting portion 29 is located between the first end portion 241 and the second end portion 242 in the first direction d1 of the container main body 20. In the container 10 in which the connecting portion 29 is located between the first end portion 241 and the second end portion 242 in the first direction d1, the connecting portion 29 is attached to the second end portion 242 in the first direction d1. A container 10 extending to the position is also included. In the examples shown in FIGS. 1 and 2, the connecting portion 29 extends linearly in the second direction d2.

Further, in the examples shown in FIGS. 1 to 4, the second end portion 242 of the container body 20 is closed. In the example shown in FIG. 2, the second end portion 242 is closed by the joining portion 22 formed by joining the film material 23.

The container body 20 is configured such that the above-mentioned take-out opening is formed on the second end 242 side of the connecting portion 29 in the first direction d1.

About the joint portion 22 in the case where a take-out opening is formed on the second end portion 242 side of the connection portion 29 in the first direction d1 by peeling off the facing portions of the film material 23 from each other in a part of the joint portion 22. , Further explanation. The portion of the joint portion 22 located on the second end portion 242 side of the connection portion 29 in the first direction d1 is referred to as the first joint portion 22b, and the portion of the joint portion 22 other than the first joint portion 22b is the first. It is referred to as a two-joint portion 22c. In this case, as an example, the first joint portion 22b has a sealing strength of at least partially 3N / 15 mm or more and 6N / 15 mm or less. For example, the above-mentioned easy-joint portion 22a is formed as a part of the first joint portion 22b, and the seal strength of the easy-joint portion 22a is 3N / 15 mm or more and 6N / 15 mm or less. As a result, in the portion of the first joint portion 22b having the above-mentioned sealing strength, the portions facing each other of the film material 23 can be easily separated from each other to form the take-out opening. Therefore, the take-out opening can be formed on the second end 242 side of the connection portion 29 in the first direction d1. In the example shown in FIG. 2, the easy joining portion 22a is located at the second end portion 242. Here, the seal strength of the joint portion 22 is measured in accordance with JIS Z 0238: 1998. As the measuring instrument, a tensile tester RTC-1310A with a constant temperature bath manufactured by Orientec can be used.

The start portion 26 in the case where the container main body 20 is cut by the start portion 26 to form the take-out opening on the second end portion 242 side of the connection portion 29 in the first direction d1 will be further described. In this case, the container body 20 has a start portion 26 which is a start position for cutting the container body on the second end portion 242 side of the connection portion 29 in the first direction d1. In the example shown in FIG. 2, the start portion 26 includes a notch. As a result, the film material 23 can be cut with the start portion 26 as the cutting start position, and the take-out opening can be formed on the second end portion 242 side of the connection portion 29 in the first direction d1.

Further, the above-mentioned accommodating portion 21 is located on the first end portion 241 side of the connecting portion 29 in the first direction d1. In the examples shown in FIGS. 1 and 2, the connecting portion 29 has a first end portion side end portion 29b, which is an end portion on the first end portion 241 side in the first direction d1. Further, the connecting portion 29 has a second end portion side end portion 29c, which is an end portion on the second end portion 242 side in the first direction d1. In the examples shown in FIGS. 1 and 2, the shapes of the first end side end portion 29b and the second end portion side end portion 29c are linear shapes extending in the second direction d2. The accommodating portion 21 is located closer to the first end portion 241 than the first end portion side end portion 29b of the connecting portion 29 in the first direction d1.

Next, the spreading portion 40 will be described. As shown in FIGS. 1 to 4, the spreading portion 40 is connected to the connecting portion 29 of the container body 20. Hereinafter, the portion of the spreading portion 40 that is connected to the container body 20 is also referred to as a spreading connecting portion 41. In the illustrated example, the spread portion 40 has a first spread portion 40A and a second spread portion 40B. As described above, the container body 20 has a pair of facing portions made of the film material 23. The first spreading portion 40A is attached to one of the pair of facing portions, and the second spreading portion 40B is attached to the other of the pair of facing portions. When the container 10 immersed in the medium 51 is observed from the vertical direction, the planned take-out opening portion 16 is located between the first spreading portion 40A and the second spreading portion 40B.

The spreading portion 40 has a spreading main body portion 43 that spreads in a direction intersecting the first direction d1 when the container 10 is immersed in the medium 51 which is the liquid 511 from the first end portion 241 side of the container main body 20. .. In the example shown in FIG. 4, the spreading main body 43 spreads in the second direction d2 perpendicular to the first direction d1. In the example shown in FIG. 4, the spreading portion 40 is manufactured as a separate member from the container main body 20 and attached to the container main body 20. Although not shown, the container body 20 and the spreading portion 40 may be integrally molded. For example, the spreading portion 40 may be a film that is integrated with the film material 23 forming the container body 20 and is folded back with respect to the film material 23 at the second end portion 242. The material of the spreading portion 40, particularly the spreading main body portion 43, is not particularly limited, but for example, a material having a specific gravity smaller than that of the medium 51 used for thawing the inclusion C, typically less than 1 which is lighter than water. It is a material with specific gravity. Like the container body 20, the spread portion 40 is preferably resistant to both high temperatures during thawing and low temperatures during freezing.

In the illustrated example, the spreading portion 40 has a resin film 45 connected to the connecting portion 29 of the container body 20. In the examples shown in FIGS. 1 and 3, the film 45 is laminated on the outer surface of the container body 20. As the material of the film 45, various resin materials described as the materials used for the container body 20 can be used. In particular, the film 45 is preferably made of the same material as the container body 20 in order to enable the film 45 to be stably bonded to the container body 20 by heat welding. Further, the thickness of the film 45 forming the spreading portion 40 can be, for example, 1 μm or more and 2 mm or less. The spreading portion 40 may be a member having rigidity.

In the example shown in FIG. 3, the spreading portion 40 has symmetry, particularly rotational symmetry, when observed from the first direction d1. When the spread portion 40 has rotational symmetry in the observation from the first direction d1, the spread portion 40 is, for example, twice symmetric. The spread portion 40 may have line symmetry when observed from the first direction d1.

In the example shown in FIGS. 1 to 4, the container C is housed inside the container body 20 in which the first end 241 and the second end 242 are closed, and the container 5 containing the container 5 Is formed. The container C housed inside the container 10 is, for example, a biological substance, specifically, a cell suspension containing cells cultured in a sterile state or cells before culturing.

Next, a method of accommodating the container C inside the container 10 to manufacture the container 5 containing the container will be described. As an example, the case where the inclusion C is a cell suspension will be described. FIG. 5 is a perspective view showing the container 10 before the container C is housed inside. Further, FIG. 6 is a front view showing the container 10 before the container C is housed inside. The storage portion 21 of the container 10 shown in FIGS. 5 and 6 is formed in the container main body 20 via a portion of the container main body 20 located on the second end portion 242 side of the connection portion 29 in the first direction d1. It leads to the outside. The container body 20 of the container 10 shown in FIGS. 5 and 6 has an opening 28 for communicating the accommodating portion 21 to the outside on the second end portion 242 side of the connecting portion 29 in the first direction d1. Therefore, the accommodating portion 21 communicates with the outside of the container main body 20 through the opening 28. The opening 28 is formed between the film materials 23 facing each other. The opening 28 shown in FIG. 5 is a portion left in a partially unbonded state when the facing portions of the film material 23 are joined to form the joint portion 22. In this case, the container 5 containing the container 5 is subjected to a supply step of supplying the container C to the container 21 through the opening 28 of the container 10 shown in FIGS. 5 and 6 and a sealing step of sealing the opening 28. Can be manufactured. As shown in FIG. 6, the container body 20 may have the above-mentioned start portion 26 at a stage before sealing the opening 28.

When the container C is a cell suspension, in the supply process, the container C is grade A in a sterile drug manufacturing area in which bacteria that damage cells are eliminated by using an isolator, a cell culture processing center, or the like. It is supplied to the accommodating unit 21 in the classified environment. The mass of the container C housed inside the container body 20 will be described. FIG. 7 is a diagram showing the outline of the cross section of the container 10 when the container 10 is floated on the medium 51 as shown in FIG. 4, and the range of the spread portion 40 and the like is shown by hatching. As shown in FIG. 7, the volume of the spreading portion 40 (the volume of the portion with the hatched diagonal line) is V ₁ , including the inside of the portion of the container body 20 where the connecting portion 29 is located in the first direction d1. The total volume of the volume and the volume of the accommodating portion 21 including the inside (the volume of the portion with the hatched dots) is V ₂ , the mass of the container 10 is W _1, and the medium 51 which is the liquid 511. Let the density be ρ. In this case, the mass of the inclusion C is not more than or equal to the value of ρ (V ₁ + V ₂ ) − W ₁ .

Next, in the sealing step, the opening 28 of the container body 20 is sealed in a closed environment. As an example, the opening 28 is sealed by joining the facing portions of the film material 23 in the opening 28 with a heat seal to form a joint 22 that closes the opening 28 as shown in FIG. It will be stopped. The portions of the film material 23 facing each other at the opening 28 are joined so that the opening joint 22d formed by joining the film material 23 at the opening 28 has a sealing strength of 3N / 15 mm or more and 6N / 15 mm or less. You may. In this case, the opening joint 22d becomes the above-mentioned easy joint 22a. Then, it becomes easy to peel off the facing portions of the film material 23 from each other in the opening joint portion 22d to form the take-out opening. As a method for forming the opening joint portion 22d having a sealing strength in the above range, the method for forming the above-mentioned easy joint portion 22a can be used. In this way, the containment section 21 is maintained in a sterile closed state, and the containment C containing cells is held in the containment section 21.

The method for manufacturing the container 5 containing the contained material may further include a step of immersing the container 10 containing the contained material C in a refrigerant such as liquid nitrogen or allowing the container 10 to stand in a liquid nitrogen gas phase. As a result, the container C in the container 10 can be stored in a frozen state until it is used. Hereinafter, the frozen content C is also referred to as a frozen content.

The container 10 has an expanding portion 40 in addition to the container main body 20 that defines the accommodating portion 21. In particular, the illustrated container 10 includes a first spreading portion 40A which is a first floating member and a second spreading portion 40B which is a second floating member. However, the spreading portion 40 is a film 45 arranged along the outer surface of the container main body 20, and does not increase the size of the container 10. Therefore, the installation of the spreading portion 40 does not have a great influence on the storage of cells in the storage portion 21 of the container body 20 and the storage of the container 10. Further, it is possible to effectively prevent the spreading portion 40 from interfering with the container C when the container C is taken out from the container 10.

In the container 5 containing the container, which is manufactured by the above method and includes the container 10 and the frozen container located inside the container 10, 50% by mass or more of the frozen container located inside the container 10. However, it is preferable that it is housed in the storage unit 21. Of the frozen contents located inside the container 10, the proportion of the frozen contents contained in the storage portion 21 is more preferably 70% by mass or more, and further preferably 90% by mass or more. preferable. When the container 10 is floated on the medium 51, the frozen container is located inside the container 21 that comes into contact with the medium 51, rather than being located inside the container 10 other than the container 21. By heat exchange with the medium 51, it is thawed faster. Therefore, as described above, if the proportion of the frozen content contained in the storage unit 21 is larger than a certain level, the frozen content can be thawed particularly quickly.

The frozen contents contained in the container 10 are thawed before use. A thawing method for thawing the frozen container contained in the container 21 of the container 10 will be described. Examples of the frozen contents contained in the container 10 include frozen cells, electrolyte infusions such as physiological saline, sugar injections such as glucose, blood preparations, antibiotics, proteinaceous drugs such as antibodies, low molecular weight proteins, and hormones. Peptide drugs, nucleic acid drugs, cell drugs, vaccines to prevent various infectious diseases, steroids, insulin, anticancer agents, proteolytic enzyme inhibitors, analgesics, antipyretic analgesics, anti-inflammatory agents, anesthetics, fat emulsions, etc. Examples thereof include antihypertensive agents, vasodilators, injection solutions for correcting electrolytes such as sodium heparin chloride and potassium lactate, and suspensions containing liquids and fine particles such as vitamins and contrast agents.

As an example, a case where the frozen inclusion is a frozen cell will be described. The frozen cells are, for example, frozen cell suspensions. The cell suspension is, for example, a solution in which cells are suspended in a cell cryopreservation solution. When the frozen containment is frozen cells, the thawing method for thawing the frozen containment can be said to be a method for producing a cell preparation, in which the cell preparation is produced by thawing the frozen cells. In this case, the cell preparation is derived from, for example, hepatoma cells, hepatocytes which are parenchymal cells of the liver, cupper cells, endothelial cells such as vascular endothelial cells and corneal endothelial cells, fibroblasts, osteoblasts, osteoblasts, and root membrane. Epithelial cells such as cells, epidermal keratinized cells, tracheal epithelial cells, gastrointestinal epithelial cells, cervical epithelial cells, corneal epithelial cells and other epithelial cells, mammary gland cells, pericite, smooth muscle cells and myocardial cells and other muscle cells , Renal cells, pancreatic Langerhans islet cells, nerve cells such as peripheral nerve cells and optic nerve cells, cartilage cells, bone cells, or stem cells, ES cells (embryonic stem cells), iPS cells (artificial pluripotent stem cells), etc. To. Examples of stem cells include undifferentiated bone marrow mesenchymal stem cells, hematopoietic stem cells, vascular stem cells, nerve stem cells, small intestinal stem cells, adipose stem cells, skin stem cells, periodontal tissue stem cells, hairy stem cells, corneal ring stem cells, visceral stem cells and the like. Be done. The frozen container contained in the container 21 is thawed by heat exchange with the medium 51 which is the liquid 511.

The method for thawing the frozen container includes a thawing step in which the container 10 is immersed in a medium 51 which is a liquid 511 from the first end portion 241 side of the container body 20. At this time, the container 10 obtains buoyancy by sinking a part of the container body 20 and at least a part of the spreading portion 40 below the interface 51a of the medium 51, and obtains buoyancy to the second end portion 242 of the container body 20. It floats on the medium 51 in a state of facing upward. Here, as shown in FIG. 4, when the container 10 is immersed in the medium 51 held in the medium holding container 50 from the first end 241 side of the container body 20, the container 21 of the container body 20 Floats on the medium 51 in a state where the container body 20 is immersed in the medium 51 and the planned outlet opening portion 16 of the container body 20 is not immersed in the medium 51. Therefore, it is possible to suppress the adhesion of the medium 51 to the planned take-out opening portion 16 while thawing the frozen container contained in the storage section 21 by heat exchange with the medium 51. As a result, when the take-out opening is formed in the take-out opening planned portion 16 and the contained material C is taken out through the take-out opening, it is possible to prevent the contained material C from being contaminated by the medium 51.

The medium 51 is not particularly limited as long as it can thaw the frozen contents, but is, for example, liquid 511, particularly water. The temperature of the medium 51 is not particularly limited as long as the frozen contents can be thawed. As an example, when the cryocontainer is frozen cells and the cell preparation is produced by thawing the frozen cells, the temperature of the medium 51 is, for example, 30 ° C. or higher and 40 ° C. or lower, typically 37 ° C. Is.

As shown in FIG. 7, the volume of the expanding portion 40 is V ₁ , the volume of the container body 20 including the inside of the portion where the connecting portion 29 is located in the first direction d1, and the volume of the accommodating portion 21 including the inside. Let V ₂ be the total volume with the volume and W ₁ be the mass of the container 10. Further, let ρ be the density of the medium 51 which is the liquid 511. In this case, it is preferable that the following equation (4) holds.

When the medium 51 is water, it is preferable that the following equation (1) is established, where the density of water is ρ _W. The water density ρ _W is, for example, the density of water at 37 ° C., for example, 1 g / cm ³ .

The action and effect of the establishment of the equation (4) will be described. Suppose that the container 10 is immersed in the medium 51 which is the liquid 511 up to the end of the spreading portion 40 on the second end 242 side in the first direction d1, that is, up to the position of the alternate long and short dash line L4 shown in FIG. think about. In this case, the value of V ₁ + V ₂ corresponds to the volume of the portion of the volume of the container 10 including the inside, which is located below the interface 51a of the medium 51. The buoyancy acting on the container 10 in a state of being immersed in the medium 51 up to the position of the one-point chain line L4 is represented by ρ (V ₁ + V ₂ ) g, where g is the gravitational acceleration. Assuming that the container 10 does not contain the container C inside, the gravity acting on the container 10 is represented by W ₁ g. Here, when the equation (4) holds, the buoyancy ρ (V ₁ + V ₂ ) g in the state of being immersed in the medium 51 up to the position of the alternate long and short dash line L4 becomes larger than the gravity W ₁ g. Therefore, when the container C is not housed inside the container 10, the container 10 floats in a state where the portion 242 side of the second end portion 242 of the spreading portion 40 is not immersed in the medium 51.

Further, consider a case where the container 10 houses the container C inside to form the container 5 containing the container. In this case, if the mass of the container C housed inside the container 10 is ρ (V ₁ + V ₂ ) -W ₁ or less, the container 5 containing the container is immersed in the medium 51 up to the position of the alternate long and short dash line L4. The buoyancy that acts is greater than the gravity that acts on the container 5 containing the contents. Therefore, the container 5 containing the container floats in a state where the portion 242 side of the second end portion 242 of the spreading portion 40 is not immersed in the medium 51. From the above, when the formula (4) is established and the mass of the contained material C contained in the container 10 is equal to or less than the value of ρ (V ₁ + V ₂ ) -W ₁ , the planned take-out opening portion 16 is reached. The adhesion of the medium 51 can be suppressed more effectively.

Next, the action and effect of the spreading portion 40 when the container 10 is floated on the medium 51 as shown in FIG. 4 will be described. First, as shown in FIG. 8, when a general container 10'having a cylindrical shape is floated on the medium 51, a case where the container 10'is tilted will be considered. In the example shown in FIG. 8, the floating center B of the container 10'is located on the side where the container 10'is tilted (right side in FIG. 8) with respect to the center of gravity G of the container 10'. In this case, a force for eliminating the inclination of the container 10', that is, a stability force acts on the container 10', depending on the positional relationship between the center of gravity G and the buoyancy center B.

Hereinafter, the intersection M of the axis of symmetry of the container 10', particularly the axis of rotational symmetry of the container 10'having a cylindrical shape, and the line of action L3 of the buoyancy P2 acting on the container 10'is also referred to as a metacenter M. .. In other words, whether or not the stability works on the container 10'is determined by the positional relationship between the center of gravity G and the metacenter M. In the example shown in FIG. 8, since the buoyancy center B is located on the side where the container 10'is tilted from the center of gravity G, at the intersection of the line of action L3 of the buoyancy P passing through the buoyancy center B and the axis of symmetry L1. A metacenter M is located above the center of gravity G. If the metacenter M is above the center of gravity G as in the example of FIG. 8, a moment of force that eliminates the inclination of the container 10'is generated.

The stability acts to rotate the container 10'by the action of the downward gravity P1 and the upward buoyancy P2 on the container 10'. The magnitude P of the stability can be expressed by the following equation (5), where the distance between the gravitational action line L2 and the buoyancy action line L3 shown in FIG. 8 is w2 and the mass of the container 10'is m. ..

Further, as shown in FIG. 9, when the container 10'floating on the medium 51 is tilted, the center of gravity G is on the side where the container 10'is tilted with respect to the floating center B (right side in FIG. 9). Consider the case where you have moved to. In this case, as shown in FIG. 9, the metacenter M, which is the intersection of the action line L3 of the buoyancy P passing through the buoyancy center B and the axis of symmetry L1, is located below the center of gravity G. When the metacenter M is below the center of gravity G as in the example of FIG. 9, a moment of force that further tilts the container 10'is generated, and the stability force cannot be exerted on the container 10'.

From FIGS. 8, 9 and (5), in order for the container to stably exert a sufficient stability, the shape of the container is such that the buoyancy B is better when the container is tilted. It can be understood that it is preferable that the container has a shape that is easier to move to the tilted side than the center of gravity G. Further, in order to make the shape of the container such that the center of gravity G is difficult to move, it is preferable that the position of the center of gravity G in the container is low.

Next, suppose that the container 10 according to the present embodiment is tilted from the state of floating on the medium 51 as shown in FIG. 4 to one side as shown in FIG. 10. In FIG. 10, the container 10 is tilted to the left side of the figure. In this case, on the tilted side of the container 10, the portion of the spreading portion 40 that was located above the interface 51a of the medium 51 in FIG. 10 sinks below the interface 51a. Further, on the side opposite to the tilted side of the container 10, the portion of the spreading portion 40 located below the interface 51a of the medium 51 in FIG. 10 rises above the interface 51a. As a result, the volume of the portion of the volume of the container 10 including the inside, which is located below the interface 51a of the medium 51, is greatly increased on the side where the container 10 is tilted, and the side opposite to the side where the container 10 is tilted. Will decrease significantly in. In the example shown in FIG. 10, the volume of the portion of the medium 51 below the interface 51a increases significantly on the left side where the container 10 is tilted, and decreases significantly on the right side opposite to the side where the container 10 is tilted. doing. Therefore, the buoyancy center of the container 10 moves significantly to the tilted side of the container 10 as compared with before the container 10 tilts. As a result, when the container 10 is tilted to one side, the buoyancy can be largely moved to the tilted side, and the container 10 can stably exert the stability. As a result, the state in which the container 10 is floating on the medium 51 so that the planned take-out opening portion 16 of the container body 20 is not immersed in the medium 51 can be further stabilized.

Further, the spreading portion 40 has symmetry, particularly rotational symmetry, when observed from the first direction d1. Since the spreading portion 40 has the above shape, when the container 10 is floating on the medium 51 as shown in FIG. 4, the container 10 faces the second end portion 242 of the container body 20 upward. Can be made more stable. Further, as shown in FIG. 4, when the container 10 is floating on the medium 51, even when the container 10 is tilted in a plurality of different directions, the stability force of the container 10 is restored by the action of the spreading portion 40. Makes it easier to work.

Further, in the above-mentioned specific example, the ratio X (%) represented by the following formula (2) is preferably 0.3% or more. When the ratio X (%) is in the above range, the volume V ₁ of the spreading portion 40, the volume including the inside of the portion of the container body 20 where the connecting portion 29 is located in the first direction d1, and the inside. The ratio of the volume V ₁ of the spreading portion 40 to the sum of the total volume V ₂ of the volume of the accommodating portion 21 including the above can be sufficiently increased. Therefore, when the container 10 floating on the medium 51 is tilted, the floating center of the container 10 is greatly moved, and the stability of the container 10 is exerted on the container 10 in a stable manner. Can be done quickly.

Further, in the above-mentioned specific example, the value obtained by dividing the volume V ₁ of the expanding portion 40 by the area of the connecting surface 29a forming the boundary between the expanding portion 40 and the connecting portion 29 is 0.15 (mm ³ / mm). ² ) or more. Thereby, the volume V ₁ of the spreading portion 40 can be sufficiently increased in the ratio with the area of the connecting surface 29a. Therefore, when the container 10 floating on the medium 51 is tilted, the floating center of the container 10 is greatly moved, and the stability of the container 10 is exerted on the container 10 in a stable manner. Can be done quickly.

Further, in the above-described specific example, the spreading portion 40 is a portion other than the connecting portion 29 of the container main body 20 when the container 10 is immersed in the medium 51 which is the liquid 511 from the first end portion 241 side of the container main body 20. Protruding away from. As a result, the spreading portion 40 and the accommodating portion 21 of the container body 20 are separated from each other. Therefore, as shown in FIG. 4, the accommodating portion 21 and the medium 51 are brought into contact with each other, and the contained object C and the medium accommodated in the accommodating portion 21 are brought into contact with each other. Heat exchange with 51 can be efficiently generated.

Further, in the above-mentioned specific example, it is preferable that the accommodating portion 21 is configured to increase the efficiency of heat exchange between the contained object C accommodated in the accommodating portion 21 and the medium 51. For example, the thickness of the film material 23 in the accommodating portion 21 is preferably 1 mm or less. The thickness of the film material 23 is more preferably 0.5 mm or less, and more preferably 0.26 mm or less. Further, the thermal conductivity of the film material 23 in the accommodating portion 21 is preferably 0.1 W / m · K or more. This allows the frozen containment to be thawed faster by heat exchange with the medium 51.

Further, in the above-mentioned specific example, it is preferable that the surface area of the accommodating portion 21 is large. Since the surface area of the accommodating portion 21 is large, the area of contact between the accommodating portion 21 and the medium 51 can be increased, and the efficiency of heat exchange between the accommodating portion C and the medium 51 can be increased. This allows the frozen containment to be thawed faster. As an example, the dimension t3 of the accommodating portion 21 in the first direction d1 shown in FIG. 2 is larger than the dimension t4 of the accommodating portion 21 in the second direction d2 perpendicular to the first direction d1. In one specific example described above, the dimension t4 corresponds to the dimensions of the first side 20a and the second side 20b. The dimension t3 is more preferably 1.1 times or more, and more preferably 1.3 times or more the dimension t4. By defining the relationship between the dimension t3 and the dimension t4 as described above, the surface area of the accommodating portion 21 on the side surface can be increased, and the surface area of the accommodating portion 21 can be sufficiently increased.

According to the container 10 having the storage unit 21 capable of increasing the efficiency of heat exchange between the container C and the medium 51, for example, the frozen container C of 1000 ml or less is thawed within 8 minutes. obtain. In addition, 15 ml or less of the container C can be thawed within 3 minutes.

The accommodating portion 21 preferably has rotational symmetry in a cross section perpendicular to the first direction d1. The accommodating portion 21 according to the present embodiment has a shape that is symmetrical twice in a cross section perpendicular to the first direction d1. By having the housing portion 21 having the above shape, when the container 10 is floating on the medium 51 as shown in FIG. 4, the container 10 faces the second end portion 242 of the container body 20 upward. Can be made more stable. Further, it is possible to suppress the occurrence of a portion inside the accommodating portion 21 that is particularly far from the medium 51, and to transfer the heat from the medium 51 to the accommodating object C more evenly.

Further, the connecting portion 29 is preferably located between the center of gravity of the container body 20 and the planned take-out opening portion 16 in the first direction d1. As a result, the position of the center of gravity of the container 10 can be set to a sufficiently low position (sufficiently on the side of the first end portion 241). Therefore, when the container 10 floating on the medium 51 is tilted, the center of gravity of the container 10 becomes more difficult to move, and the medium is particularly stable in a state where the planned take-out opening portion 16 is not immersed in the medium 51. It can float on 51.

After thawing of the frozen contents is completed, the contents C inside the container 10 are put into use as follows. First, the container 10 is pulled up from the medium 51. After that, a take-out opening for taking out the contents C is formed in the take-out opening planned portion 16. Next, the thawed container C in the container 10 is taken out from the take-out opening. Then, the taken-out container C is put into use.

In JP-A-2018-110539 and JP-A-2001-70402, which have been introduced as prior arts, in order to prevent the liquid from adhering to the planned outlet opening of the container, the container is externally attached prior to thawing the container. It is stored in a bag. After thawing the contents by immersing the outer bag in the liquid, the container is taken out from the outer bag. Compared with such a conventional technique, according to the present embodiment, the work of storing the container 10 containing the frozen contents in the outer bag before the thawing work and the work of storing the thawed contents C in the container 10 It is possible to omit the work of taking out the container 10 from the outer bag before the work of taking out the container 10 from the take-out opening. As a result, the preparation time until the frozen container C is used can be significantly shortened, and the preparation work can be reduced. It can be said that such an action effect is particularly suitable when a thawed biological substance, for example, a cell such as a cell suspension or a cell sheet is applied to the human body.

Further, the spreading portion 40 can exert a protective function of the container body 20. For example, when the container 5 containing the contained material in a frozen state is dropped, it is assumed that the container 5 containing the contained material collides with the floor, desk, processing table, etc., and receives an impact from the floor, desk, processing table, or the like. In such a case, the spreading portion 40 receives the impact preferentially over the container body 20, and the impact on the container body 20 can be prevented.

In one specific example described above, an example in which one spreading portion 40 is provided on one main surface of the container body 20 is shown, but two or more spreading portions 40 are provided on one main surface. May be good. For example, two or more spreading portions 40 may be attached to the container main body 20 via different spreading connecting portions 41 on one main surface. Further, the spread connection portion 41 of one spread portion 40 and the spread connection portion 41 of the other spread portion 40 may be overlapped with each other to connect the plurality of spread portions 40 to the container main body 20. According to such an example, when the spreading portion 40 is the floating member 401, the buoyancy of the container 10 can be further strengthened. In addition, the protection function of the spreading portion 40 can be improved.

Further, as described above, the spreading portion 40 may be a floating member 401 formed of a material having a specific gravity smaller than that of the medium 51 used for thawing the inclusion C. The floating member 401 is typically made of a material having a specific gravity of less than 1, which is lighter than water. In this case, the floating member 401 floats on the medium 51 with the container body 20 at least partially immersed in the medium 51. Then, in the floating member 401, when the container body 20 is at least partially immersed in the medium 51, when the contained object C is taken out from the accommodating portion 21, the accommodating portion 21 is communicated to the outside. It promotes that the part 16 is located outside the medium 51.

From the viewpoint of effectively preventing the medium 51 from adhering to the take-out opening during thawing of the container C, the floating member 401 is connected to the container body 20 in the vicinity of the take-out opening or the take-out opening planned portion 16. Is preferable. Preferably, as shown in FIGS. 3 and 4, the connection position of the floating member 401 to the container body 20 is located between the take-out opening or the take-out opening planned portion 16 and the frozen container C in the accommodating portion 21. positioned. Further, when the container 10 has a plurality of floating members 401 and the container 10 immersed in the medium 51 is observed from the vertical direction, the take-out opening or the planned take-out opening 16 is between the plurality of floating members 401. It is preferable to be located.

In the illustrated example, the floating member 401 has a resin film 45 connected to the container body 20. The floating member 401 made of the film 45 has a spread connecting portion 41 which is a part thereof. The floating member 401 is joined to the container body 20 at the spreading connection portion 41. The spread connecting portion 41 may be bonded to the container body 20 by heat welding, or may be bonded to the container body 20 via a bonding layer made of an adhesive or an adhesive. The spreading connection portion 41 is formed by a linear edge portion of the resin film 45, particularly a linearly extending edge portion. The spread connection portion 41 extends along the take-out opening or the take-out opening planned portion 16. Further, the spread connection portion 41 is located between the take-out opening or the take-out opening planned portion 16 and the frozen container C in the accommodating portion 21. More strictly, the spread connection portion 41 is located between the take-out opening or the planned take-out opening 16 and the containment C in a direction along a straight line connecting the take-out opening or the planned take-out opening 16 and the inclusion C. Is located in. Further, the spread connection portion 41 is located between the take-out opening or the take-out opening planned portion 16 and the center of gravity of the container body 20. The center of gravity of the container body 20 used in the present specification refers to the center of gravity of the container containing the container C immediately before thawing, that is, the center of gravity of the container 5 containing the container immediately before heat exchange with the medium 51. In particular, the spread connection portion 41 is such that the take-out opening or the planned take-out opening 16 and the center of gravity of the container main body 20 are aligned with each other in the direction along a straight line connecting the take-out opening or the planned take-out opening 16 and the center of gravity of the container body 20. It is located in between.

The floating member 401 further has an extending portion 42 extending from the expanding connecting portion 41. Here, the extending portion 42 refers to, for example, the expanding main body portion 43 when the expanding portion 40 corresponds to the floating member 401. The extending portion 42 extends along the outer surface of the container body 20. In particular, the extending portion 42 extends to the side away from the taking-out opening or the taking-out opening planned portion 16 and extends from the connecting portion 41. The film 45 can be bent by bending or bending. As shown in FIG. 4, the film 45 can be separated from the container main body 20 at the extending portion 42 by bending.

As described above, the film 45 forming the floating member 401 is made of a material having a specific gravity smaller than that of the medium 51. Therefore, when the container 10 is immersed in the medium 51, the floating member 401 made of the film 45 floats on the medium 51. Further, the film 45 forming the floating member 401 is preferably flexible or flexible. As will be described later, in order to apply an appropriate elastic force to the floating member 401, the thickness of the film 45 forming the floating member 401 may be thicker than the thickness of the film material 23 forming the container body 20.

Next, the operation of the container 10 provided with the above floating member 401 will be described.

As shown in FIG. 4, immediately before using the biological material, the container 10 is immersed in the medium 51 held in the medium holding container 50. At this time, the container 10 is immersed in the medium 51 from the side away from the take-out opening or the take-out opening planned portion 16. Here, when the spreading portion 40 is the floating member 401, the first spreading portion 40A and the second spreading portion 40B attached to the pair of facing portions of the film material 23 forming the container body 20 are smaller than the medium 51. The film 45 has a specific gravity. The film 45 is attached to the container body 20 at the edge on the side close to the take-out opening or the take-out opening planned portion 16. Therefore, as shown in FIG. 4, the film 45 is curved away from the container body 20 and floats on the surface of the medium 51. That is, the film 45 forming the first spreading portion 40A and the film 45 forming the second spreading portion 40B extend so as to be separated from the container main body 20 and spread on the surface of the medium 51.

The spread connection portion 41 connected to the container body 20 of the floating member 401 is located in the vicinity of the take-out opening or the take-out opening planned portion 16. Further, the spreading connection portion 41 of the floating member 401 is an outlet opening that allows the contained object C accommodated in the accommodating portion 21 and the accommodating portion 21 to be communicated to the outside of the container body 20 when the contained object C is taken out from the accommodating portion 21. It is located between the part or the planned take-out opening part 16. Further, the spread connection portion 41 of the floating member 401 is located between the center of gravity of the container body 20 and the take-out opening or the take-out opening planned portion 16. Therefore, the take-out opening or the take-out opening planned portion 16 is maintained above the surface of the medium 51 in the vertical direction, as shown in FIG. That is, the medium 51 is effectively prevented from adhering to the take-out opening.

In the illustrated example, since the film 45 is used as the floating member 401, not only the buoyancy caused by the specific gravity of the film 45 but also the elastic force trying to resist the bending of the floating member 401 will be taken out or planned to be taken out. The portion 16 acts to float from the medium 51. Further, the film 45 spreads on the surface of the medium 51. In particular, the floating member 401 has a pair of films 45 extending on both sides in a direction orthogonal to the flat container body 20 as a first spreading portion 40A and a second spreading portion 40B. With each of these configurations, the posture of the container 10 floating on the surface of the medium 51 is stabilized, and the take-out opening or the planned take-out opening 16 is held on the surface of the medium 51, and the medium 51 to the take-out opening is held. Can be effectively prevented from adhering.

According to the container 10 in which the spreading portion 40 is the floating member 401 described above, the container 10 used when thawing the container C containing a biological substance such as cells by contact with the medium 51 is taken out. It has a container body 20 having an accommodating portion 21 that communicates to the outside through an opening, and a floating member 401 connected to the outer surface of a portion of the container body 20 that forms the accommodating portion 21. The material used for the floating member 401 has a specific gravity smaller than that of the medium 51. Therefore, when the container 10 is immersed in the medium 51 in order to thaw the container C containing the biological substance, the floating member 401 promotes the extraction opening or the planned extraction opening 16 to be located outside the medium 51. .. Therefore, it is possible to effectively prevent the medium 51 from adhering to the take-out opening and the take-out opening from being contaminated by the medium 51.

Further, the floating member 401 is connected to the container body 20 in the vicinity of the take-out opening or the take-out opening planned portion 16 that allows the accommodating portion 21 to be communicated to the outside when the contained object C is taken out from the accommodating portion 21. According to such a floating member 401, when the container 10 is immersed in the medium 51, it is possible to effectively prevent the take-out opening or the planned take-out opening 16 from being immersed in the medium 51.

In one specific example of the above-described embodiment, the floating member 401 has a resin film 45 provided on the outer surface of the container body 20 and connected to the container body 20 only at the spreading connection portion 41 which is a part thereof. ing. The resin film 45 is separated from the spreading connection portion 41 connected to the container main body 20 and the take-out opening or the take-out opening planned portion 16 that allows the accommodating portion 21 to be communicated to the outside when the contained object C is taken out from the accommodating portion 21. It has an extending portion 42 extending to the side and extending from the connecting portion 41. The extending portion 42 can be separated from the container main body 20 by bending. According to such a floating member 401, the take-out opening or the take-out opening planned portion 16 is moved above the medium 51 due to the buoyancy caused by the specific gravity of the resin film 45 and the elastic force of the resin film 45. It can be positioned stably. As a result, when the container 10 is immersed in the medium 51, it is possible to more effectively prevent the take-out opening or the planned take-out opening 16 from being immersed in the medium 51. Further, since the floating member 401 is a resin film 45 connected to the container body 20, it is possible to effectively prevent the container 10 from becoming large in weight. Further, it is possible to effectively prevent the floating member 401 from interfering with the removal of the contained object C from the container 10.

In one specific example of the above-described embodiment, the spreading connection portion 41 is a linearly extending edge portion of the resin film 45. As a result, it is possible to effectively prevent the resin film 45 from interfering with the removal of the contained material C from the container 10.

In one specific example of the above-described embodiment, the container body 20 is made of two resin film materials 23 in which the overlapping and facing portions are joined, or a resin film in which the folded and facing portions are joined. It has a film material 23. The floating member 401 includes a first spreading portion 40A and a second spreading portion 40B provided on a pair of facing portions of the resin film material, respectively. According to such a floating member 401, the posture of the container 10 when the container 10 is immersed in the medium 51 can be stabilized. In particular, it is suitable for stabilizing the posture of the container 10 having a flat shape. As a result, it is possible to more effectively prevent the take-out opening or the take-out opening scheduled portion 16 from being immersed in the medium 51.

Although one embodiment has been described by a plurality of specific examples, these specific examples are not intended to limit one embodiment. The above-described embodiment can be implemented in various other specific examples, and various omissions, replacements, changes, and additions can be made without departing from the gist thereof.

Hereinafter, an example of modification will be described with reference to the drawings. In the following description and the drawings used in the following description, the same reference numerals as those used for the corresponding parts in the above-mentioned specific examples are used for the parts that can be configured in the same manner as the above-mentioned specific examples, and the same reference numerals are used, and duplicate explanations are given. Is omitted.

First, in the above-mentioned specific example, the resin film 45 forming the spreading portion 40 was arranged so as to spread on the outer surface of the container main body 20, and one edge portion thereof was joined to the container main body 20. However, as shown in FIGS. 11 to 13, the resin film 45 is bent so as to be folded back, and the overlapped portion of the folded film 45 is spread out and joined to the container body 20 as a connecting portion 41. May be good. In the example shown in FIGS. 11 to 13, the film 45 forming the spreading portion 40 is a rectangular film, and is bent so as to be folded around an axis orthogonal to the longitudinal direction at an intermediate portion in the longitudinal direction thereof. Has been done. Then, the film 45 bent so as to be folded back is arranged on the outer surface of the container body 20 and fixed to the container body 20. In this example, a pair of edges of the rectangular film 45 in the longitudinal direction, each extending in the lateral direction, are overlapped to form a spread connecting portion 41 joined to the container body 20. There is. Other configurations, for example, the position of the spread connection portion 41 on the container body 20 and the like can be the same as the above-described specific example described with reference to FIGS. 1 to 4.

Also in this modification, as shown in FIG. 13, the spreading portion 40 can suppress the adhesion of the medium 51 to the planned take-out opening portion 16. Further, when the spreading portion 40 is the floating member 401, the rigidity of the floating member 401 is increased, and the floating member 401 that pushes the surface of the medium 51 to lift the take-out opening or the planned take-out opening 16 of the container 10. The elastic force can be increased. Therefore, when the container 10 is immersed in the medium 51 in order to thaw the container C containing the biological substance, the take-out opening or the planned take-out opening 16 can be stably held above the medium 51. As a result, it is possible to effectively prevent the medium 51 from adhering to the take-out opening and the take-out opening from being contaminated by the medium 51.

Further, as shown in FIGS. 14 to 15, the container 10 may have a tubular member 30 connected to the container body 20 so as to communicate with the accommodating portion 21. In the illustrated example, the tubular member 30 is connected to the second end 242 side of the container body 20. In the illustrated example, the two tubular members 30 are provided on the container body 20 separately from the container body 20. The tubular member 30 passes through the joint portion 22, and one end of each tubular member 30 is located inside the container body 20. However, the tubular member 30 may be integrally molded with the container body 20. Further, the end portion of the tubular member 30 may be held by the joint portion 22, and the tubular member 30 may be opened to the accommodating portion 21 at the end portion held by the joint portion 22. Further, the number of tubular members 30 held in one container 10 may be one or three or more. In another example from the illustrated example, the number of tubular members 30 held in one container 10 is three. According to the container 10 having the tubular member 30, the tubular member 30 can be used to aseptically connect to another container to construct a closed environment, for example, a cell culture system. That is, it can be aseptically connected to various treatment containers such as a cell storage container, a culture solution storage container, a culture container, and a drainage container while effectively reducing contamination from the outside (outside air).

For example, when the container 10 is not connected to another container or the like via the tubular member 30, the outer end 31 of the end of the tubular member 30 located outside the container body 20 is closed. There is. In other words, the end of the tubular member 30 on the side separated from the container body 20 is sealed. By sealing the end portion of the container body 20, the accommodating portion 21 of the container body 20 can be maintained in a closed state. On the other hand, at the time of use, for example, an aseptic connection device can be used to aseptically connect the tubular member 30 to the tube material or the like that communicates with other containers or the like. As an example, the outer end portion 31 is closed by a sealing portion formed by joining the facing side surfaces of the tubular member 30. In this case, it is preferable that the tubular member 30 is made of a material having a low melting temperature and high softness so that the end portion of the tubular member 30 can be easily sealed by heat sealing. As a specific example, a polyolefin resin such as polyethylene or polypropylene, a polyvinyl chloride, an ethylene vinyl acetate copolymer resin, and a thermoplastic elastomer (C-FLEX, etc.) can be used as the material of the tubular member 30.

Further, when the outer end portion 31 is closed, the container 10 may further include a lid material for closing the outer end portion 31, although not shown. In one example, the lid material is a member that can be attached and detached from the outer end portion 31 and has a cap shape. Further, the lid material may be a member adhered to the outer end portion 31 so that it can be peeled off from the outer end portion 31. Further, the lid material may be a member made of a material such as a glass tube that can be easily punctured, for example, rubber.

Further, when the outer end portion 31 is closed, although not shown, the tubular member 30 may have a thin-walled portion that closes the outer end portion 31. The thin-walled portion is configured to facilitate puncture with a syringe or puncture with a syringe needle. The thickness of the thin portion is preferably 1 mm or less, more preferably 0.5 mm or less, and even more preferably 0.3 mm or less.

As an example, an aseptic connection port may be provided at the end of the tubular member 30 on the side away from the container 10 so that it can be aseptically connected to a tube material or the like that communicates with another container or the like during use. The sterile connection port may have a plurality of connection ports so that it can be connected to a plurality of appliances. Alternatively, a port cover (not shown) may be provided as a lid material so as to cover the opening provided at the tip of the tubular member 30, so that the planned take-out opening portion 16 may be formed. The opening opened by removing the port cover is used as the take-out opening, and a syringe with an injection needle is connected to the take-out opening to take out the container C or inject a substance (for example, a diluent) into the container 21. May be good. The syringe with an injection needle may be directly connected to the take-out opening, or may be connected to the take-out opening via a connector (for example, an adapter used for an infusion set or the like) for connecting the take-out opening and the syringe with an injection needle. In the latter case, a connector is connected to the take-out opening.

In the example shown in FIGS. 14 to 15, the tubular member 30 forms an take-out opening that allows the accommodating portion 21 to pass to the outside when the contained object C is taken out from the accommodating portion 21. In this example as well, the spreading portion 40 can suppress the adhesion of the medium 51 to the planned take-out opening portion 16. Further, in this example, the spreading portion 40 is a floating member 401, which is connected to the container body 20 in the vicinity of the position where the tubular member 30 is connected to the container body 20. Strictly speaking, the tubular member 30 is connected to the joint portion 22 forming one edge of the container body 20, and the spreading portion 40, which is the floating member 401, is in the vicinity of the joint portion 22 to which the tubular member 30 is connected. Is connected to the container body 20 at. Further, the spread connection portion 41 of the floating member 401 extends linearly along the longitudinal direction of the joint portion 22 to which the tubular member 30 is connected. Even in such an example, as shown in FIG. 15, when the container 10 is immersed in the medium 51, it is possible to effectively prevent the take-out opening or the planned take-out opening 16 from being immersed in the medium 51. Can be done.

A method of accommodating the container C inside the container 10 shown in FIGS. 14 to 15 will be described. FIG. 16 is a perspective view showing a container 10 provided with a tubular member 30 before the container C is housed therein. In the example shown in FIG. 16, the outer end 31 of the tubular member 30 is open. First, a supply step is performed in which the container C is supplied to the storage section 21 via the open tubular member 30 of the container 10. Next, a sealing step of sealing the outer end portion 31 is performed. As a result, the container C can be stored inside the container 10.

Although not shown, the container body 20 is provided on the inner surface of the film material 23 on the second end portion 242 side of the connecting portion 29 in the first direction d1, and the first fitting portion and the first fitting portion that can be fitted to each other. It may have a second fitting portion. As an example, the first fitting portion is provided on the inner surface of the first surface 23d of the film material 23, and extends from the third side 20c to the fourth side 20d in the second direction d2. Further, the second fitting portion is provided on the inner surface of the second surface 23e of the film material 23, and extends from the third side 20c to the fourth side 20d in the second direction d2. In this case, the second end portion 242 is closed by fitting the first fitting portion and the second fitting portion. Further, by removing the fitting between the first fitting portion and the second fitting portion, the accommodating portion 21 is moved to the outside of the container body 20 via the space between the first fitting portion and the second fitting portion. I can communicate. The fact that the accommodating portion 21 communicates with the outside of the container body 20 means that the accommodating space inside the accommodating portion 21 communicates with the outside of the container body 20.

As an example, the container body 20 has a chuck tape extending from the third side 20c to the fourth side 20d in the second direction d2, and also has a first fitting portion and a second fitting portion as a part of the chuck tape. have. In this case, of the chuck tape, the portion of the film material 23 provided on the inner surface of the first surface 23d corresponds to the first fitting portion. Further, of the chuck tape, the portion of the film material 23 provided on the inner surface of the second surface 23e corresponds to the second fitting portion. The chuck tape may be a so-called slide chuck type chuck tape. In this case, the chuck tape slides in the extending direction of the first fitting portion and the second fitting portion to fit the first fitting portion and the second fitting portion, or the first fitting portion. It also has a slider that disengages the mating with the second mating portion.

Further, the spreading portion 40 has a spreading main body portion 43 that spreads in a direction intersecting the first direction d1 when the container 10 is immersed in the medium 51 which is the liquid 511 from the first end portion 241 side of the container main body 20. The spreading auxiliary portion 46 may be provided at the end of the spreading main body 43 on the side away from the container main body 20. FIG. 17 is a diagram showing a state when the container 10 is floated on the medium 51 in a modified example in which the spreading portion 40 has the spreading auxiliary portion 46. As shown in FIG. 17, when the container 10 is immersed in the medium 51 from the first end portion 241 side of the container main body 20, the spreading auxiliary portion 46 is the first end portion of the spreading main body portion 43 in the first direction d1. It is located on the 241 side and extends in the first direction d1.

The spreading auxiliary portion 46 may be integrally formed with the spreading main body portion 43, or may be manufactured as a separate member from the spreading main body portion 43 and attached to the spreading main body portion 43. As the material of the spreading auxiliary portion 46, the same material as the material of the spreading main body portion 43 described above can be used. When the spreading auxiliary portion 46 is manufactured as a member separate from the spreading main body portion 43, the spreading auxiliary portion 46 may or may not have rigidity.

In order to explain the action and effect of the container 10 shown in FIG. 17, consider a case where the container 10 floating on the medium 51 is tilted to one side (for example, the left side of FIG. 17). In this case, in the container 10 having the spreading auxiliary portion 46, the buoyancy center is more likely to move to the side where the container 10 is tilted as compared with the container 10 shown in FIGS. 1 to 4. Therefore, when the container 10 floating on the medium 51 is tilted, the floating center of the container 10 is greatly moved, and the stability of the container 10 is exerted on the container 10 in a stable manner. Can be done quickly. When the container 10 is tilted due to a wave generated in the medium 51 due to the stability, the tilt of the container 10 is eliminated. Further, the container 10 is placed on the medium 51 so that the planned take-out opening portion 16 is not immersed in the medium 51 while maintaining a constant inclination even when the inclination is not eliminated by the stability. May continue to float on. Also in this case, it is possible to suppress the adhesion of the medium 51 to the planned take-out opening portion 16.

FIG. 18 is a diagram showing the outline of the cross section of the container 10 when the container 10 is floated on the medium 51 as shown in FIG. 17, and also shows the range of the spread portion 40 and the like by hatching. As shown in FIG. 18, the volume of the spread portion 40 (the total volume of the portion with the hatched diagonal line and the portion with the hatched horizontal line) is V ₁ , and the volume of the container body 20 in the first direction d1. V ₂ and the volume of the connecting portion 29 including the inside of the part located, the sum of the volume of the housing portion 21 including an interior volume (volume of the portion hatched dot is _attached), the spreading auxiliary section 46 Let V ₃ be the volume of (the volume of the part with the hatched horizontal line). In this case, the ratio Y (%) represented by the following formula (3) is preferably 0.2% or more. When the ratio Y (%) is in the above range, the volume V ₁ of the spreading portion 40, the volume including the inside of the portion of the container body 20 where the connecting portion 29 is located in the first direction d1, and the inside. The ratio of the volume V ₃ of the spreading auxiliary portion 46 to the sum of the total volume V ₂ with the volume of the accommodating portion 21 including the above can be sufficiently increased. Therefore, when the container 10 floating on the medium 51 is tilted, the floating center of the container 10 is greatly moved, and the stability of the container 10 is exerted on the container 10 in a stable manner. Can be done quickly.

Further, the spreading portion 40 may have a bag-shaped portion 44 containing the content B that expands when warmed. FIG. 19 is a perspective view showing a container 10 in a modified example in which the spreading portion 40 has a bag-shaped portion 44. FIG. 20 is a side view showing a state in which the container 10 is floated on the medium 51 and the bag-shaped portion 44 of the container 10 of FIG. 19 is warmed. In the example shown in FIGS. 19 and 20, the spreading portion 40 has two bag-shaped portions 44 for accommodating the content B in the bag inside 44a. The content B housed in the bag-shaped portion 44 is, for example, air.

Since the spread portion 40 has the bag-shaped portion 44 containing the content B, the volume V ₁ of the spread portion 40 is increased by being warmed and decreased by being cooled. As a result, when the container 5 containing the contained material C in the frozen state is stored, as shown in FIG. 19, the container 5 containing the contained material is stored in a state where the volume V ₁ of the spreading portion 40 is small. can do. Therefore, the space required for storing the container 5 containing the contents can be reduced. Further, when the container 5 containing the container is floated on the medium 51 and the container C is thawed, the content B is warmed by heat exchange with the medium 51, and as shown in FIG. 20, the volume V of the spreading portion 40 ₁ increases. Therefore, when the container C is thawed, the volume V ₁ of the spreading portion 40 is sufficiently increased so that the container 10 does not immerse the planned outlet opening portion 16 of the container body 20 in the medium 51. It is possible to further improve the action of the spreading portion 40, which is to make the state of floating above more stable.

When the container 10 is floated on the medium 51, the bag-shaped portion 44 is in a state of standing up toward the side away from the container body 20 as shown in FIG. 20 due to the contents B being warmed and expanding. May be. As a result, when the container 5 containing the container is floated on the medium 51 and the container C is thawed, the spreading portion 40 and the container 21 of the container body 20 are separated from each other. Therefore, the accommodating portion 21 and the medium 51 can be brought into contact with each other to efficiently cause heat exchange between the contained object C accommodated in the accommodating portion 21 and the medium 51.

In the content B housed in the bag-shaped portion 44, when the volume V _{1 of the} spreading portion is the first temperature of the content B, the following equation (1) does not hold, but the temperature of the content B is high. When the temperature is higher than the first temperature, the temperature may be expanded so that the equation (1) holds. Further, when the volume V ₁ of the spreading portion 40 is the temperature of the content B at the first temperature, the X (%) of the following formula (2) is less than 5%, but the temperature of the content B is the second. When it is the temperature, it may be expanded so that X (%) of the formula (2) becomes 5% or more. Further, when the temperature of the content B is the first temperature, the value obtained by dividing the volume V ₁ of the spreading portion 40 by the area of the connecting surface 29a does not become 5 (mm ³ / mm ² ) or more, but the content B When the temperature of is the second temperature, the volume V ₁ of the spreading portion 40 may be expanded so that the value obtained by dividing by the area of the connecting surface 29a is 5 (mm ³ / mm ² ) or more. The first temperature is, for example, the temperature of the content B when the container 5 containing the container containing the frozen container C is stored, and is, for example, -196 ° C. or higher and −20 ° C. or lower. The second temperature is, for example, the temperature of the content B when the container 5 containing the container is floated on the medium 51 and the container C is thawed. For example, when the frozen contents C are frozen cells, the second temperature is a temperature suitable for culturing the cells, for example, 20 ° C. or higher and 40 ° C. or lower, preferably 37 ° C.

Further, the container 10 may further include a string portion 60 having one end connected to a part of the expanding portion 40 and the other end connected to a part of the container body 20. FIG. 21 is a perspective view showing a container 10 in a modified example including the string portion 60. In the example shown in FIG. 21, one end of the string portion 60 is connected to the end portion 40c of the spreading portion 40 on the side opposite to the side connected to the connecting portion 29. Further, in the example shown in FIG. 21, the other end of the string portion 60 is connected to the first end portion 241 of the container body 20. Although not shown, the other end of the string portion 60 may be connected to the side surface of the container body 20 on the first end portion 241 side of the connection portion 29 in the first direction d1. When the container 10 is floated on the medium 51, the string portion 60 moves the spreading portion 40 so that the end portion 40c of the spreading portion 40 does not move more than a certain amount toward the second end portion 242 side in the first direction d1. To limit. When the container 10 is floated on the medium 51, it is possible to prevent the end 40c of the spreading portion 40 from moving away from the medium 51. Therefore, the volume of the portion of the spreading portion 40 that sinks below the interface 51a can be made larger. As a result, the volume of the portion of the container body 20 that sinks below the interface 51a can be relatively suppressed, and the adhesion of the medium 51 to the planned take-out opening portion 16 can be suppressed.

Further, in the above-described embodiment and each modification, an example in which the connecting portion 29 extends linearly in the second direction d2 has been described, but the form of the connecting portion 29 is not limited to this. As an example, the connecting portion 29 has a curved shape as shown in FIG. In the example shown in FIG. 22, the connecting portion 29 has an arch-shaped shape that is convex toward the second end portion 242 side. Further, as shown in FIG. 23, the connecting portion 29 may be provided in a part of the portion where the expanding portion 40 is located in the second direction d2. In the example shown in FIG. 23, the spreading portion 40 and the container body 20 are fixed by eyelets 25. In the example shown in FIG. 23, the container body 20 has a through hole 22e in the first joint portion 22b, and the expanding portion 40 and the container body 20 are fixed by eyelets inserted into the through hole 22e. In this case, of the container body 20, the annular portion fixed to the spreading portion 40 by the eyelet 25 corresponds to the connecting portion 29.

It is considered that the spreading portion 40 is easily broken at the boundary between the portion connected to the connecting portion 29 and the portion not connected to the connecting portion 29. Here, as shown in FIG. 22, the connecting portion 29 has a curved shape, or as shown in FIG. 23, the connecting portion 29 is provided in a part of a portion where the expanding portion 40 is located in the second direction d2. By doing so, the following effects can be obtained. The boundary between the portion of the spreading portion 40 that is connected to the connecting portion 29 and the portion that is not connected to the connecting portion 29 is not formed so as to linearly cross the expanding portion 40. Therefore, the boundary between the portion of the spread portion 40 that is connected to the connection portion 29 and the portion that is not connected to the connection portion 29 crosses the spread portion 40 in a straight line. It becomes difficult to break in a straight line. As a result, the angle at which the spreading portion 40 breaks when the container 10 is floated on the medium 51 can be suppressed to a small size, and the end portion 40c of the spreading portion 40 can be prevented from moving significantly toward the second end portion 242 in the direction d1. Therefore, the volume of the portion of the spreading portion 40 that sinks below the interface 51a can be made larger. As a result, the volume of the portion of the container body 20 that sinks below the interface 51a can be relatively suppressed, and the adhesion of the medium 51 to the planned take-out opening portion 16 can be suppressed.

Further, in the above-mentioned specific example, the case where the surface of the film material 23 is flat has been described. However, the shape of the film material 23 is not limited to this. FIG. 24 is a partial cross-sectional view showing a part of a cross section of the film material 23 in one modification of the film material 23 cut in a plane perpendicular to the first direction d1. Further, FIG. 25 is a partial cross-sectional view showing a part of a cross section of the film material 23 in another modified example of the film material 23 cut in a plane perpendicular to the first direction d1. As shown in FIG. 24, the film material 23 may have a shape in which the inner surface 23a located on the inner side of the container 10 and the outer surface 23b located on the outer side of the container 10 have a plurality of groove portions 23c. The groove 23c extends, for example, in the first direction d1. As a result, the area of contact between the container C and the film material 23 and the area of contact between the film material 23 and the medium 51 can be sufficiently increased. As a result, the efficiency of heat exchange between the container C and the medium 51 can be increased.

Further, as shown in FIG. 25, one of the inner surface 23a and the outer surface 23b of the film material 23 may have a shape having a plurality of irregularities in a cross section perpendicular to the first direction d1. In the example shown in FIG. 25, the inner surface 23a has a shape having a plurality of groove portions 23c, and the outer surface 23b is a flat surface. As a result, the area of contact between the container C and the film material 23 can be sufficiently increased. Therefore, especially when the specific heat of the film material 23 is smaller than the specific heat of the container C, the heat exchange between the container C and the medium 51 is increased by increasing the efficiency of heat exchange between the container C and the film material 23. Efficiency can be increased.

Further, the accommodating portion 21 may have the accommodating portion detail 21b having a smaller dimension in the direction perpendicular to the first direction d1 than the portion of the container body 20 on the second end 242 side of the connecting portion 29. Good. FIG. 26 is a perspective view showing a state before sealing the opening 28 on the second end 242 side of the container 10 in a modified example in which the accommodating portion 21 has the accommodating portion detail 21b. In the example shown in FIG. 26, the container body 20 has a start portion 26 and an opening 28. FIG. 27 is a perspective view showing a state before sealing the opening 28 on the second end 242 side of the container 10 in another modification in which the accommodating portion 21 has the accommodating portion detail 21b. In the example shown in FIG. 27, the container 10 includes a tubular member 30, and the outer end 31 of the tubular member 30 is open.

As shown in FIGS. 26 and 27, the dimension t5 of the housing portion detail 21b in the second direction d2 perpendicular to the first direction d1 is the dimension of the portion of the container body 20 on the second end portion 242 side of the connecting portion 29. It is smaller than (for example, dimension t6). If the dimensions of the portion of the container body 20 on the second end 242 side of the connection portion 29 are not constant, the dimension t5 is the portion of the container body 20 on the second end 242 side of the connection portion 29. Less than the maximum size. The volume of the housing portion detail 21b is preferably 70% or more of the total volume of the housing portion 21.

Since the dimension t5 of the housing detail 21b is smaller than the dimension t6, when the container 10 is floated on the medium 51, it is located in the center of the inside of the housing detail 21b in the direction perpendicular to the first direction d1. The distance between the container C and the medium 51 can be kept small. As a result, heat exchange between the container C housed in the storage section detail 21b and the medium 51 can be efficiently generated. Further, since the dimension t6 of the portion of the container body 20 on the second end 242 side of the connection portion 29 is larger than the dimension t5, the container body 20 is taken out to the portion of the container body 20 on the second end 242 side of the connection portion 29. When forming the opening, a larger take-out opening can be formed. This makes it easier to take out the container C from the inside of the container 10 through the take-out opening. In particular, the operation of inserting the tip of a pipette or the like into the inside of the container 10 through the take-out opening and dispensing the contained material C using the pipette or the like can be facilitated.

Further, as shown in FIG. 28, a label 70 may be attached to the spreading portion 40. The label 70 is attached to the spread 40 by, for example, being adhered to the spread 40 with an adhesive or by heat welding. On one side surface of the label 70, for example, information such as the contents of the container C housed in the container 10 and how to use the container 10 is described. By attaching the label 70 to the spreading portion 40, when the container 10 is floated on the medium 51 as shown in FIG. 29, the surface on which the information of the label 70 is described faces upward. Therefore, the user of the container 10 can visually recognize the information on the label 70 while the container 10 is floating on the medium 51. Further, by attaching the label 70 to the spreading portion 40, the components of the adhesive and the like when the adhesive is used for attaching the label 70 enter the inside of the container 10 as compared with the case where the label 70 is attached to the film material 23. It is more suppressed that it is stored.

Further, although not shown, the container 10 may further include a mark portion located on the second end portion 242 side of the connection portion 29 in the first direction d1. The mark portion is, for example, a protruding portion of the film material 23 provided on the outer surface of the container 10. The mark portion may be a groove-shaped portion of the film material 23 provided on the outer surface of the container 10. The mark portion indicates, for example, that the take-out opening should be formed on the second end portion 242 side of the mark portion in the first direction d1.

As yet another modification, in the above-mentioned container 10, the container 21 of the container body 20 is maintained in an aseptic state, and the container C containing a biological substance is directly stored in the container body 20. However, it is not limited to this example. The container C containing the biological substance is stored in an inner bag separate from the container 10, the container C is cryopreserved in the inner bag, and the inner bag containing the container C is stored in the container 10 described above. The defrosting operation using the medium 51 may be performed. In this example, the container 10 may be used only during the thawing operation. Even in such a use example, since the medium 51 is effectively prevented from adhering to the take-out opening of the container 10, it is effective that the medium 51 adheres to the inner bag when the inner bag is taken out from the container 10. Can be avoided.

Further, in the above-mentioned example, an example in which the container 10 contains a biological substance is shown, but the present invention is not limited to this example. It may be required to avoid mixing of the medium used for heat exchange, adhesion of the medium, contact with the medium, and the like also for various inclusions containing no biological substance. As an example, when heat exchanges food with heated or cooled water, it may be desired that water adhere to the food. The container 10 having the spreading portion 40 according to the above-described embodiment can be applied to various containers without being limited to the containers containing biological substances, and can be applied to various containers during heat exchange of the medium. It is possible to effectively prevent the medium from coming into contact with, adhering to, or mixing with the contained material.

That is, the container 10 is a container used for heat exchange between the container C and the medium 51, and is connected to the container body 20 having the storage section 21 for storing the container C and the outer surface of the container body 20. It may have a spreading portion 40 which is a floating member 401 having a specific gravity smaller than that of the medium 51. Further, the container 10 has a container main body 20 having a storage portion 21 for accommodating the container C, and a spread portion 40 connected to the outer surface of the container main body 20, and the spread portion 40 is inside the storage portion 21. Even if it is connected to the container body 20 at a position between the contained container C and the take-out opening that allows the stored container C to pass to the outside when the container C is taken out from the container 21. Good. According to these containers 10, it is possible to solve the problem of effectively preventing the medium 51 for thawing the container C from adhering to the container C of the container 10.

Next, the present disclosure will be described in more detail by way of examples, but the present disclosure is not limited to the description of the following examples as long as the gist of the present disclosure is not exceeded.

(Example 1)
As the first embodiment, as shown in FIG. 4, a container 5 containing a container 10 having a form shown in FIGS. 1 to 4 and a frozen container C housed inside the container 10 is provided as a medium 51 as shown in FIG. Floated on top. Then, the container 5 containing the container was floated on the medium 51 in a state where the second end portion 242 of the container body 20 was not immersed in the medium 51, and it was evaluated whether the container 5 containing the container floated stably.

The material of the container 10 was made of polyethylene. Further, the size t7 of the container body 20 in the first direction d1 shown in FIG. 2 was 12 cm. Further, the dimension t4 of the accommodating portion 21 in the second direction d2 was 9.5 cm. Further, when the container 10 is in the state shown in FIG. 2, the dimension t1 of the spreading portion 40 in the first direction d1 is 1 cm. Further, the dimension t2 of the spreading portion 40 in the second direction d2 was 8 cm. Further, the dimension t8 of the spread connection portion 41 in the first direction d1 was 0.5 cm. Further, the distance t9 between the first joint portion 22b and the spread connection portion 41 in the first direction d1 was 0.5 cm. The width t10 of the joint portion 22 was 0.5 cm. The thickness of the film material 23 was 0.026 cm.

For the container 10, as the volume _{V 2} shown in FIGS. 7 and 18, the volume of the film member 23 than the second end side end portion 29c of the connecting portion 29 in the first direction d1 positioned in the first end portion 241 side , The volume of the container 10 and the total volume were calculated. The volume of the vessel 10 is also referred to as the volume _{V 4.} The volume V ₄ was calculated based on the mathematical formula described in "Film for Food Packaging (Published by Daily Report Publishing)" by Hiroshi Osuga, that is, the following formula (6). A in the formula (6) is the distance between the joint portion 22 located on the third side 20c and the joint portion 22 located on the fourth side 20d in the second direction d2. Further, S of the formula (6) is located and joined to the first end portion 241 side of the second end portion side end portion 29c when the container 10 is observed from the direction perpendicular to the surface of the film material 23. This is the area of the portion where the portion 22 is not formed. In the case of the container 10 shown in FIG. 2, S is the product of the above-mentioned a and the distance t11 between the second end side end portion 29c in the first direction d1 and the joint portion 22 located on the first side 20a. expressed.

The value obtained by dividing the volume V ₁ of the spreading portion 40 by the area of the connecting surface 29a was 0.10 (mm ³ / mm ² ). The ratio X (%) represented by the above formula (2) was 0.22%. Further, since the container 10 of Example 1 does not have the spreading auxiliary portion 46, the ratio Y (%) represented by the above formula (3) was 0%.

The medium 51 on which the container 10 was floated was water at 37 ° C. When the density ρ of the medium 51 which is the liquid 511 is 1 g / cm ³ , the value of ρ (V ₁ + V ₂ ) -W ₁ which is the left side of the above formula (4) is 180.56 (g). It was.

Container C was a frozen cell in which the cell suspension was frozen. The mass of the contained material C contained in the container 10 was 15.00 (g).

(Example 2)
As the second embodiment, except for the points described below, with respect to the container 5 containing the container 5 similar to the container 5 containing the container of the first embodiment, the container 5 containing the container 5 is stable on the medium 51 by the same method as in the first embodiment. I evaluated whether it floats on. In Example 2, the dimension t1 was 1.5 cm. The value obtained by dividing the volume V ₁ by the area of the connecting surface 29a was 0.16 (mm ³ / mm ² ). The ratio X (%) represented by the above formula (2) was 0.33%. The value of ρ (V ₁ + V ₂ ) -W _{1 on} the left side of the above equation (4) was 180.57 (g).

(Example 3)
As the third embodiment, except for the points described below, with respect to the container 5 containing the container 5 similar to the container 5 containing the container of the first embodiment, the container 5 containing the container 5 is stable on the medium 51 by the same method as in the first embodiment. I evaluated whether it floats on. In Example 3, the dimension t1 was 3 cm. The value obtained by dividing the volume V ₁ by the area of the connecting surface 29a was 0.31 (mm ³ / mm ² ). The ratio X (%) represented by the above formula (2) was 0.66%. The value of ρ (V ₁ + V ₂ ) −W _{1 on} the left side of the above equation (4) was 180.59 (g).

(Example 4)
As Example 4, except for the points described below, with respect to the container 5 containing the container 5 similar to the container 5 containing the container of Example 1, the container 5 containing the container is stable on the medium 51 by the same method as in Example 1. I evaluated whether it floats on. In Example 4, the dimension t1 was 6 cm. The value obtained by dividing the volume V ₁ by the area of the connecting surface 29a was 0.62 (mm ³ / mm ² ). The ratio X (%) represented by the above formula (2) was 1.31%. The value of ρ (V ₁ + V ₂ ) -W _{1 on} the left side of the above equation (4) was 180.64 (g).

(Example 5)
As Example 5, except for the points described below, with respect to the container 5 containing the container 5 similar to the container 5 containing the container of Example 1, the container 5 containing the container is stable on the medium 51 by the same method as in Example 1. I evaluated whether it floats on. In Example 5, the dimension t1 was 8 cm. The value obtained by dividing the volume V ₁ by the area of the connecting surface 29a was 0.83 (mm ³ / mm ² ). The ratio X (%) represented by the above formula (2) was 1.75%. The value of ρ (V ₁ + V ₂ ) -W _{1 on} the left side of the above equation (4) was 180.67 (g).

(Example 6)
As Example 6, except for the points described below, with respect to the container 5 containing the container 5 similar to the container 5 containing the container of Example 1, the container 5 containing the container is stable on the medium 51 by the same method as in Example 1. I evaluated whether it floats on. In Example 6, the container 10 shown in FIGS. 1 to 4 was further provided with the spreading assisting portion 46 shown in FIGS. 17 and 18. In Example 6, the dimension t1 was 1.5 cm. The value obtained by dividing the volume V ₁ by the area of the connecting surface 29a was 0.25 (mm ³ / mm ² ). The ratio X (%) represented by the above formula (2) was 0.54%. The ratio Y (%) represented by the above formula (3) was 0.21%. The value of ρ (V ₁ + V ₂ ) -W _{1 on} the left side of the above equation (4) was 180.20 (g).

(Evaluation results)
In any of Examples 1 to 6, it was confirmed that the container 5 containing the container floats stably on the medium 51 in a state where the second end portion 242 of the container body 20 is not immersed in the medium 51. Therefore, according to the container 10 of the present disclosure, it was found that the adhesion of the medium 51 to the planned take-out opening portion 16 located on the second end portion 242 side of the container main body 20 is suppressed.

In order to evaluate the stability of the container 5 containing the contents of Examples 1 to 5 when floating on the wavy medium 51, the following evaluation was further performed. First, a water bath (manufactured by AS ONE Corporation, trade name "Thermal Robo TR-2AR") having a water tank filled with water as a medium 51 and a jet pump (discharge amount: 10 L / min) was prepared. Next, the jet pump was operated to make the water in the aquarium ruffle. Next, the container 5 containing the container was floated on the water surface at a distance of about 25 cm from the jet pump so that the second end portion 242 was not immersed in water, and it was evaluated whether the container 5 containing the container floated stably. The temperature of the water was 37 ° C.

As a result of evaluation on the wavy medium 51, the value obtained by dividing the volume V ₁ by the area of the connecting surface 29a is less than 0.15 (mm ³ / mm ² ), and the ratio X (%) is less than 0.3%. The container 5 containing the contents of Example 1 did not float stably, and the second end portion 242 came into contact with water. On the other hand, Examples 2 to 5 in which the value obtained by dividing the volume V ₁ by the area of the connecting surface 29a is 0.15 (mm ³ / mm ² ) or more and the ratio X (%) is 0.3% or more. The container 5 containing the contents of the above floated stably. Therefore, according to the container 10 of the present disclosure, the value obtained by dividing the volume V ₁ by the area of the connecting surface 29a is 0.15 (mm ³ / mm ² ), or the ratio X (%) is 0.3. It was found that the container 5 containing the contained material can be floated on the medium 51 in a particularly stable manner when the content is set to% or more, in a state where the second end portion 242 of the container body 20 is not immersed in the medium 51.

The following evaluations were further performed on the containers 5 containing the contents of Examples 2 and 6 in order to evaluate the stability when floating on the wavy medium 51 in particular. First, a water bath (manufactured by AS ONE Corporation, trade name "Thermal Robo TR-2AR") having a water tank filled with water as a medium 51 and a jet pump (discharge amount: 10 L / min) was prepared. Next, the jet pump was operated to make the water in the aquarium ruffle. Next, the container 5 containing the container was floated around the jet pump so that the second end 242 was not immersed in water, and it was evaluated whether the container 5 containing the container floated stably. The temperature of the water was 37 ° C.

As a result of the evaluation on the rippling medium 51, the container 5 containing the container of Example 2 having no spreading auxiliary portion 46 did not float stably, and the second end portion 242 came into contact with water. On the other hand, the container 5 containing the container of Example 6 having the spreading auxiliary portion 46 floated stably. Therefore, it was found that the spreading auxiliary portion 46 can float the container 5 containing the contained material on the medium 51 in a particularly stable manner in a state where the second end portion 242 of the container body 20 is not immersed in the medium 51. ..

C Container 5 Container containing container 10 Container 16 Scheduled take-out opening 20 Container body 21 Storage 21b Storage details 22 Joint 22a Easy joint 22b First joint 22c Second joint 22d Opening joint 23 Film material 241 1st end 242 2nd end 25 Eyelet 26 Start part 27 Cutting part 28 Opening part 29 Connection part 29a Connection surface 29b 1st end side end part 30 Cylindrical member 31 External end 40 Spreading part 401 Floating member 40A First 1 Spreading part 40B Second spreading part 40c End part 41 Spreading connection part 42 Extension part 43 Spreading body part 44 Bag-shaped part 45 Film 46 Spreading auxiliary part 50 Medium holding container 51 Medium 511 Liquid 51a Interface 60 String part 70 Label

Claims (30)

A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
The second end portion is closed by a joint portion formed by joining the film materials.
The container body has a start portion that includes a notch on the second end side of the connection portion in the first direction and serves as a starting position for cutting the container body, or
A container in which the first joint portion of the joint portion located closer to the second end portion side than the connection portion in the first direction has at least a partial sealing strength of 3N / 15 mm or more and 6N / 15 mm or less. A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
Further, a tubular member connected to the second end side of the container body so as to communicate with the storage portion is further provided.
A container in which the outer end portion of the end portion of the tubular member located outside the container body is closed. A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
The container body is further provided with a first fitting portion and a second fitting portion which are provided on the inner surface of the film material and can be fitted to each other on the second end side of the connecting portion in the first direction. Have and
The second end portion is a container that is closed by fitting the first fitting portion and the second fitting portion. A container for containing biological substances
It is a film material having a first surface and a second surface facing each other, and has a film material in which at least a part of a portion where the first surface and the second surface face each other is joined, and also has a first end portion and a film material. A container body having a second end facing the first end in the first direction.
The container body includes a spreading portion connected to a connecting portion located between the first end portion and the second end portion in the first direction.
The container body is located on the first end side of the connection portion in the first direction with respect to the first end side end portion, and has a storage portion for accommodating the contents inside.
The container is a container that leads to the outside of the container body through a portion of the container body that is located closer to the second end portion than the connection part in the first direction. Further, a tubular member connected to the second end side of the container body so as to communicate with the storage portion is further provided.
The container according to claim 4, wherein the outer end portion of the end portion of the tubular member located outside the container body is open. The container body has an opening formed between the film materials facing each other on the second end side of the connection portion in the first direction to allow the storage portion to pass to the outside of the container body. And
The container according to claim 4, wherein the container body has a start portion including a notch on the second end side of the connection portion in the first direction and serves as a starting position for cutting the container body. The container body has an opening formed between the film materials facing each other on the second end side of the connection portion in the first direction to allow the storage portion to pass to the outside of the container body. And
The fourth aspect of claim 4, wherein when the film material is joined in the opening, the opening joint formed by joining the film material in the opening has a sealing strength of 3N / 15 mm or more and 6N / 15 mm or less. Container. The container body is further provided with a first fitting portion and a second fitting portion which are provided on the inner surface of the film material and can be fitted to each other on the second end side of the connecting portion in the first direction. Have and
The container according to claim 4, wherein the accommodating portion communicates with the outside of the container body via the first fitting portion and the second fitting portion. 1. The container has details of the container, which has a smaller size in the second direction perpendicular to the first direction than the portion of the container body on the second end side of the connection. The container according to any one of 8 to 8. The container according to any one of claims 1 to 9, wherein the size of the storage portion in the first direction is larger than the size of the storage portion in the second direction perpendicular to the first direction. The container according to any one of claims 1 to 10, wherein the thickness of the film material in the housing portion of the container body is 1 mm or less. The container according to any one of claims 1 to 11, wherein the film material in the container body has a thermal conductivity of 0.1 W / m · K or more. Claims 1 to 12, wherein the spreading portion projects so as to be separated from a portion of the container body other than the connecting portion when the container is immersed in a liquid from the first end side of the container body. The container according to any one item. The volume of the spread portion is V ₁ , the total volume of the volume of the container body including the inside of the portion where the connection portion is located in the first direction and the volume of the accommodating portion is V ₂ , and the container. The container according to any one of claims 1 to 13, wherein the following formula (1) is established when the mass of the container is W ₁ and the density of water is ρ _W.
The container according to claim 14, wherein the ratio X (%) represented by the following formula (2) is 0.3% or more.
Claims 1 to 15 wherein the value obtained by dividing the volume of the spread portion by the area of the connecting surface forming the boundary between the spread portion and the connection portion is 0.15 (mm ³ / mm ² ) or more. The container according to any one item. When the container is immersed in a liquid from the first end side of the container body, the spreading portion is formed from a spreading main body portion that spreads in a direction intersecting the first direction and the container main body of the spreading main body portion. The container according to any one of claims 1 to 16, further comprising a spreading auxiliary portion connected to an end portion on a separated side. The volume of the spreading portion is V ₁ , and the total volume of the volume of the container body including the inside of the portion where the connecting portion is located in the first direction and the volume of the housing portion including the inside is V. _2, wherein the volume of the spreading auxiliary section when the V _3, is the ratio Y (%) is 0.2% or more of the following formula (3) a container according to claim 17.
The container according to any one of claims 1 to 18, wherein the spread portion has symmetry when observed from the first direction. The container according to any one of claims 1 to 19, wherein the spreading portion has a bag-shaped portion containing contents that expand when warmed. The container according to claim 20, wherein the bag-shaped portion stands up toward a side away from the container body when the contents are warmed and expanded. The container according to claim 2, further comprising a lid material for detachably closing the outer end portion. The container according to claim 2, wherein the tubular member has a thin-walled portion that closes the outer end portion. The container according to claim 2, wherein the outer end portion is closed by a sealing portion formed by joining the facing side surfaces of the tubular member. A container containing a container, comprising the container according to any one of claims 1 to 3 and a frozen container located inside the container.
A container containing a container in which 50% by mass or more of the frozen container located inside the container is stored in the container. A supply step of supplying an inclusion to the accommodation portion via the tubular member of the container according to claim 5.
A method for manufacturing a container containing an container, comprising a sealing step of sealing the outer end portion. A supply step of supplying the contained material to the contained portion through the opening of the container according to claim 6 or 7.
A method for manufacturing a container containing an inclusion, comprising a sealing step for sealing the opening. A thawing method for thawing a frozen container contained in the container according to any one of claims 1 to 3 by heat exchange with a liquid.
A thawing method comprising a thawing step of immersing the container in the liquid from the first end side of the container body. The volume of the spread portion is V ₁ , and the total volume of the volume of the container body including the inside of the portion where the connection portion is located in the first direction and the volume of the storage portion including the inside is V. _2. The thawing method according to claim 28, wherein the following formula (1) is established when the mass of the container is W ₁ and the density of the liquid is ρ.
A method for producing a cell preparation, wherein the cell preparation is produced by thawing the frozen cells housed in the container according to any one of claims 1 to 3 by heat exchange with a liquid. ,
A method for producing a cell preparation, which comprises a thawing step of immersing the container in the liquid from the first end side of the container body.