JP7409216B2 - Container, container containing contents, manufacturing method of container containing contents, thawing method, and manufacturing method of cell preparation - Google Patents

Description

The present disclosure relates to a container, a container containing a content, a method for manufacturing a container containing a content, a thawing method, and a method for producing a cell preparation.

For example, as disclosed in Patent Document 1 and Patent Document 2, containers for storing frozen contents are known. The container is used as a biological material container for storing biological materials such as cells and secreted substances from cells in a frozen state. Containers for biological materials are particularly used as containers for cells to store cultured cells in a frozen state until they are used. Immediately before use, the contents in the container are thawed by heat exchange with a suitably heated liquid along with the container. For example, if a container contains objects containing biological substances such as cells or secreted substances, the contents can be removed by immersing the container in a liquid such as water maintained at 30°C to 40°C. It will be thawed.

JP 2018-110539 Publication Japanese Patent Application Publication No. 2001-70402

For example, when thawing the contents in a container, it may be necessary to immerse a portion of the container in a liquid. In this case, there is a possibility that the stored item may be contaminated by liquid adhering to the opening for storing the stored item in the container or the opening for removing the stored item from the container.

The present disclosure has been made in consideration of these points, and an object of the present disclosure is to effectively suppress the adhesion of liquid to the opening of a container.

A container according to the present disclosure includes:
A container containing biological material,
A film material having a first face and a second face facing each other, the film material having at least a part of the part where the first face and the second face face each other are joined, and the film material has a first end and a second face. a container body having a second end opposite the first end in a first direction;
a widening part connected to a connecting part located between the first end part and the second end part in the first direction of the container main body,
The container main body has a storage part located closer to the first end than the first end of the connection part in the first direction, and stores the contents therein,
The second end is closed by a joint formed by joining the film material,
The container body includes a notch on the second end side of the connecting portion in the first direction, and has a starting portion that is a cutting start position of the container body, or
Among the joint parts, a first joint part located closer to the second end than the connection part in the first direction has a seal strength of 3 N/15 mm or more and 6 N/15 mm or less at least partially.

Another container according to the present disclosure includes:
A container containing biological material,
A film material having a first face and a second face facing each other, the film material having at least a part of the part where the first face and the second face face each other are joined, and the film material has a first end and a second face. a container body having a second end opposite the first end in a first direction;
a widening part connected to a connecting part located between the first end part and the second end part in the first direction of the container main body,
The container main body has a storage part located closer to the first end than the first end of the connection part in the first direction, and stores the contents therein,
further comprising a cylindrical member connected to the second end side of the container body so as to communicate with the accommodating part,
Among the ends of the cylindrical member, an external end located outside the container body is closed.

Another container according to the present disclosure includes:
A container containing biological material,
A film material having a first face and a second face facing each other, the film material having at least a part of the part where the first face and the second face face each other are joined, and the film material has a first end and a second face. a container body having a second end opposite the first end in a first direction;
a widening part connected to a connecting part located between the first end part and the second end part in the first direction of the container main body,
The container main body has a storage part located closer to the first end than the first end of the connection part in the first direction, and stores the contents therein,
The container main body further includes a first fitting part and a second fitting part which are provided on the inner surface of the film material closer to the second end than the connecting part in the first direction and are capable of fitting into each other. have,
The second end portion is closed by fitting the first fitting portion and the second fitting portion.

Another container according to the present disclosure includes:
A container containing biological material,
A film material having a first face and a second face facing each other, the film material having at least a part of the part where the first face and the second face face each other are joined, and the film material has a first end and a second face. a container body having a second end opposite the first end in a first direction;
a widening part connected to a connecting part located between the first end part and the second end part in the first direction of the container main body,
The container main body has a storage part located closer to the first end than the first end of the connection part in the first direction, and stores the contents therein,
The accommodating portion communicates with the outside of the container body through a portion of the container body that is located closer to the second end than the connecting portion in the first direction.

The container according to the present disclosure further includes a cylindrical member connected to the second end side of the container body so as to communicate with the accommodating part,
Among the ends of the cylindrical member, an external end located outside the container body may be open.

In the container according to the present disclosure, the container main body is formed between the facing film materials closer to the second end than the connecting part in the first direction, so as to extend the accommodating part to the outside of the container main body. It has an opening that allows it to pass through,
The container body may include a notch on the side closer to the second end than the connection portion in the first direction, and may have a starting portion that is a starting position for cutting the container body.

In the container according to the present disclosure, the container main body is formed between the facing film materials closer to the second end than the connecting part in the first direction, so as to extend the accommodating part to the outside of the container main body. It has an opening that allows it to pass through,
When the film material is joined at the opening, the opening joint formed by joining the film material at the opening may have a seal strength of 3 N/15 mm or more and 6 N/15 mm or less.

In the container according to the present disclosure, the container body is provided on the inner surface of the film material closer to the second end than the connection part in the first direction, and includes a first fitting part and a first fitting part that can be fitted to each other. It further has two fitting parts,
The accommodating part may communicate with the outside of the container main body via between the first fitting part and the second fitting part.

In the container according to the present disclosure, the accommodating portion has a smaller dimension in a second direction perpendicular to the first direction than a portion of the container body closer to the second end than the connecting portion. It may have.

In the container according to the present disclosure, the size of the accommodating part in the first direction may be larger than the size of the accommodating part in a second direction perpendicular to the first direction.

In the container according to the present disclosure, the thickness of the film material in the accommodating portion of the container body may be 1 mm or less.

In the container according to the present disclosure, the film material in the accommodating portion of the container body may have a thermal conductivity of 0.1 W/m·K or more.

In the container according to the present disclosure, the widening portion may protrude away from a portion of the container body other than the connecting portion when the container is immersed in liquid from the first end side of the container body. good.

In the container according to the present disclosure, the volume of the expanding portion is V ₁ , and the total volume of the volume of the container body including the inside of the portion where the connecting portion is located in the first direction and the volume of the accommodating portion. When V ₂ is the mass of the container, W ₁ is the mass of the container, and ρ _W is the density of water, the following equation (1) may be established.

In the container according to the present disclosure, the ratio X (%) expressed by the following formula (2) may be 0.3% or more.

In the container according to the present disclosure, a value obtained by dividing the volume of the widening portion by the area of a connecting surface forming a boundary between the widening portion and the connecting portion is 0.15 (mm ³ /mm ² ) or more. Good too.

In the container according to the present disclosure, the spreading portion includes a spreading main body portion that spreads in a direction crossing the first direction when the container is immersed in liquid from the first end side of the container main body, and a spreading main body portion that spreads in a direction crossing the first direction. and a spreading auxiliary part connected to the end of the part on the side away from the container body.

In the container according to the present disclosure, the volume of the expanding portion is V ₁ , the volume including the inside of the portion of the container body where the connecting portion is located in the first direction, and the volume of the accommodating portion including the inside. The ratio Y (%) expressed by the following formula (3) may be 0.2% or more, where V ₂ is the total volume of V 2 and V ₃ is the volume of the expansion auxiliary part.

In the container according to the present disclosure, the widening portion may have symmetry when viewed from the first direction.

In the container according to the present disclosure, the expanding portion may have a bag-like portion containing contents that expand when heated.

In the container according to the present disclosure, the bag-like portion may stand up toward a side away from the container main body as the contents are warmed and expanded.

The container according to the present disclosure may further include a lid that removably closes the outer end.

In the container according to the present disclosure, the cylindrical member may have a thin wall portion that closes the outer end.

In the container according to the present disclosure, the outer end portion may be closed by a seal portion formed by joining opposing side surfaces of the cylindrical member.

A container containing contents according to the present disclosure is a container containing contents, comprising the container described above and a frozen contents located inside the container,
Of the frozen contents located inside the container, 50% by mass or more is accommodated in the storage section.

A method for manufacturing a container containing a contained substance according to the present disclosure includes a supplying step of supplying a contained substance to the storage part via the cylindrical member of the container described above;
and a sealing step of sealing the outer end.

Another method for manufacturing a container containing a stored material according to the present disclosure includes a supplying step of supplying a stored material to the storage part through the opening of the container described above;
and a sealing step of sealing the opening.

A thawing method according to the present disclosure is a thawing method in which a frozen content stored in the storage part of the container described above is thawed by heat exchange with a liquid,
The method includes a thawing step of immersing the container in the liquid from the first end side of the container body.

In the thawing method according to the present disclosure, the volume of the expanding portion is V ₁ , the volume of the container body including the inside of the portion where the connecting portion is located in the first direction, and the volume of the accommodating portion including the inside. The following equation (1) may hold true, where V ₂ is the total volume of V 2 , W ₁ is the mass of the container, and ρ is the density of the liquid.

A method for producing a cell preparation according to the present disclosure includes producing a cell preparation by thawing frozen cells accommodated in the storage part of the container described above by heat exchange with a liquid. ,
The method includes a thawing step of immersing the container in the liquid from the first end side of the container body.

According to the present disclosure, it is possible to effectively prevent liquid from adhering to the opening of the container.

FIG. 1 is a diagram for explaining an embodiment of the present disclosure, and is a perspective view showing a specific example of a container. FIG. 2 is a front view of the container of FIG. 1. FIG. 3 is a side view of the container of FIG. 1. FIG. 4 is a diagram for explaining the function of the container shown in FIG. 1, and is a side view showing the container. FIG. 5 is a perspective view showing the container before the contents are stored therein. FIG. 6 is a front view of the container of FIG. 5. FIG. 7 is a cross-sectional view of the container of FIG. 1. FIG. 8 is a diagram showing a general container having a cylindrical shape floating on a liquid. FIG. 9 is a diagram showing a general container having a cylindrical shape floating on a liquid. FIG. 10 is a diagram for explaining the function of the container of FIG. 1, and is a side view of the container. FIG. 11 is a diagram corresponding to FIG. 1, and is a perspective view showing another specific example of the container. FIG. 12 is a side view of the container of FIG. 11. FIG. 13 is a diagram for explaining the function of the container shown in FIG. 11, and is a side view showing the container. FIG. 14 is a diagram corresponding to FIG. 2, and is a front view showing still another specific example of the container. FIG. 15 is a diagram for explaining the operation of the container shown in FIG. 14, and is a side view showing the container. FIG. 16 is a perspective view of the container of FIG. 14. FIG. 17 is a diagram corresponding to FIG. 4, and is a side view for explaining still another specific example of the container and its operation. FIG. 18 is a cross-sectional view of the container of FIG. 17. FIG. 19 is a diagram corresponding to FIG. 1, and is a perspective view showing still another specific example of the container. FIG. 20 is a diagram for explaining the function of the container of FIG. 19, and is a side view of the container. FIG. 21 is a diagram corresponding to FIG. 1, and is a perspective view showing still another specific example of the container. FIG. 22 is a diagram corresponding to FIG. 1, and is a perspective view showing still another specific example of the container. FIG. 23 is a diagram corresponding to FIG. 1, and is a perspective view showing still another specific example of the container. FIG. 24 is a partial sectional view showing still another specific example of the container. FIG. 25 is a partial cross-sectional view showing still another specific example of the container. FIG. 26 is a diagram corresponding to FIG. 2, and is a side view showing still another specific example of the container. FIG. 27 is a diagram corresponding to FIG. 2, and is a side view showing still another specific example of the container. FIG. 28 is a diagram corresponding to FIG. 1, and is a perspective view showing still another specific example of the container. FIG. 29 is a diagram for explaining the action of the container of FIG. 28, and is a side view of the container.

An embodiment of the present disclosure will be described with reference to the drawings. In addition, in the drawings attached to this specification, for convenience of illustration and ease of understanding, the scale and the vertical and horizontal dimensional ratios are appropriately changed and exaggerated from those of the actual drawings.

1 to 4 are diagrams for explaining an embodiment of the present disclosure, and show a specific example of a container.

The object-containing container 5 includes a container 10 and an object C accommodated in the container 10. The container 10 accommodates the contents C. In the present embodiment, as an example, a container 10 used when thawing a frozen content C by heat exchange with a medium 51 described later will be described. The medium 51 is, for example, a liquid 511. Further, the contained object C is, for example, a biological material. A biological substance is a substance that can exist in the body of an organism, and examples thereof include cells and secreted substances secreted from cells. The contents C accommodated in the container 10 include, as one specific example, cultured cells such as tissues of a human body, cell sheets, etc., and cell suspensions containing cells before culture. Generally, the container C containing biological materials such as cells is stored in a frozen state until used. Immediately before use, the contents C in the container 10 are immersed together with the container 10 in a moderately heated medium 51. The contained object C containing the biological material thawed by heat exchange with the medium 51 is taken out from the accommodating part of the container 10 through the take-out opening. In particular, the container 10 according to the present embodiment is designed to effectively prevent the medium 51 from adhering to the take-out opening while the contained object C is thawed using the medium 51. By preventing the medium 51 from adhering to the take-out opening, it is possible to effectively avoid contamination of the contents C taken out through the take-out opening.

First, an embodiment will be described with reference to a specific example shown in FIGS. 1 to 4. 1 to 3 are perspective, front, or side views showing a container 10 containing biological material. FIG. 4 is a diagram showing the container 10 floating on the medium 51.

As shown in FIGS. 1 to 3, the container 10 includes a container body 20 and a floating member 401 connected to the container body 20. Here, the floating member 401 corresponds to the one formed of a material having a specific gravity smaller than that of the medium 51 used for defrosting the contained object C, etc., among the spreading parts 40 described later. That is, the floating member 401 is a concept included in the spreading part 40, and it can be said that the container 10 has the spreading part 40 by having the floating member 401. Hereinafter, the portion of the container body 20 that is connected to the widening portion 40 will also be referred to as the connecting portion 29. Further, the container main body 20 has a storage section 21 that stores the contents C. It is preferable that the container main body 20 has a generally flat shape so that heat exchange between the medium 51 and the contents C can be promoted. The container body 20 is a film material 23 having a first surface 23d and a second surface 23e that face each other, and at least a part of the portion where the first surface 23d and the second surface 23e face each other is joined. has. As an example, the surface of the film material 23 is flat. In one example, the container body 20 includes two resin film materials 23 that are overlapped and joined at the edges, or one resin film material 23 that is folded back and joined at the edges. ing. When the container body 20 has two film materials 23 that are overlapped and joined at the edges, one of the two film materials 23 constitutes the first surface 23d, and the other constitutes the second surface 23e. do. Further, when the container main body 20 has a single sheet of film material 23 that is folded back and its edges are joined, the first surface 23d and the second surface 23e are part of the single film material 23, and the film material 23 are folded back to face each other. As another example, the container body 20 may include a cylindrical film material 23 whose edges are joined. In the example shown in FIG. 2, the container body 20 has a rectangular shape in plan view. The accommodation space inside the accommodation part 21 of the container body 20 is surrounded by the joint part 22 from all sides. The joint portion 22 is formed, for example, by thermally welding opposing portions of a resin film material 23. That is, the container main body 20 can be produced by overlapping two resin film materials 23 and thermally welding the four peripheral parts. The container body 20 can also be produced by folding back a sheet of resin film material 23 and thermally welding the edges on three sides. Furthermore, the container main body 20 can also be produced by closing the two openings of a cylindrical film material obtained by inflation bag making by thermal welding. This joint portion 22 allows the storage portion 21 to be closed and maintain the sterile state of the storage portion 21. However, the joining portion 22 is not limited to this example, and may include a joining layer such as an adhesive or a pressure-sensitive adhesive that joins the facing portions of the film material 23.

In the examples shown in FIGS. 1 to 4, the container main body 20 forms a take-out opening when the contained object C is taken out from the container main body 20. The take-out opening connects the accommodating portion 21 of the container body 20 to the outside of the container 10 when taking out the contents C from the container body 20. The extraction opening is formed, for example, by peeling off the facing portions of the film material 23 at the easy-to-join portion 22a, which is a part of the joining portion 22. Compared to other parts of the joint part 22, the easy-to-join part 22a is made by lowering the temperature during the thermal welding process to form the joint part 22, shortening the processing time, lowering the processing pressure, or It can be obtained by a combination of two or more selected from these. Hereinafter, the portion where the extraction opening is planned to be formed will also be referred to as the extraction opening planned portion 16.

As another example of the take-out opening, the take-out opening may be formed by cutting the container body 20 along the dashed line in FIG. 2 . In such an example, the container main body 20 may have a starting portion 26 configured to be a cutting starting position, as shown by a dashed line in FIG. 2 . The starting portion 26 may be configured as a slit or notch formed in the film material 23 forming the joint portion 22 . Furthermore, the container body 20 may have a cutting section 27 configured to facilitate cutting of the film material 23, as shown by dotted lines in FIG. The cutting portion 27 can be configured by a linearly extending thin wall portion provided on the film material 23, or a linear or linear notch or notch that does not penetrate the film material 23.

The container body 20 needs to have resistance to contact with the medium 51 while the contents C are being thawed. More specifically, the container body 20 preferably has physical properties that allow it to physically withstand an environment of at least 30°C or higher, typically 37°C. The container body 20 preferably has physical properties that can physically withstand an environment of preferably 60° C. or higher, particularly preferably 100° C. or higher, which is the boiling point of water as the typical medium 51. Moreover, it is preferable that the container main body 20 is also used for freezing processing of the contents C. That is, the container body 20 preferably has resistance to both high temperatures during thawing and low temperatures during freezing. More specifically, the container body 20 has a temperature of at least 0°C or lower, preferably -80°C or lower, more preferably -150°C or lower in a liquid nitrogen gas phase, particularly preferably -196°C or lower that can maintain nitrogen in a liquid state. It is preferable that the material has physical properties that can physically withstand the above environment. Here, "physically durable" means that the film material 23 constituting the container body 20 will not be damaged or the film material 23 will not peel off at the joint portion 22. Moreover, physically durable may mean that the container body 20 will not be damaged, the sealing performance of the container 10 will be impaired, and the sterile state inside the container 10 will not be maintained. Examples of materials used for the container body 20 include low-density polyethylene, linear low-density polyethylene, medium-density polyethylene, high-density polyethylene, ultra-high molecular weight polyethylene, nylon, polyester, polyimide, ethylene-vinyl acetate copolymer, and fluorine. Usable resins include vinyl chloride copolymers, ethylene-methyl methacrylate copolymers, ionomers, polyurethanes, polypropylene, polystyrene, polyacetals, phenolic resins, urea resins, epoxy resins, ABS resins, and polyethylene terephthalate. The illustrated container body 20 can be manufactured using a resin film material 23 made of these materials, or a resin film material 23 made of a plurality of laminated layers made of these materials.

The container body 20 will be further explained. In the example shown in FIGS. 1 to 4, the container body 20 has a first end 241 and a second end 242 opposite to the first end 241 in the first direction d1. At least the first end 241 is closed. As described above, in the example shown in FIG. 2, the container body 20 extends in the first direction d1 and has a rectangular shape in plan view. The container body 20 extends in a second direction d2 that intersects the first direction d1, and has a first side 20a and a second side 20b that are opposite to each other in the first direction d1. It has a third side 20c and a fourth side 20d. In the example shown in FIG. 2, the second direction d2 is perpendicular to the first direction d1. The first end 241 is formed by the first side 20a, and the second end 242 is formed by the second side 20b.

Furthermore, the connecting portion 29 is located between the first end 241 and the second end 242 of the container body 20 in the first direction d1. Note that in a container 10 in which the connecting portion 29 is located between the first end 241 and the second end 242 in the first direction d1, the connecting portion 29 is located between the second end 242 in the first direction d1. Also included is a container 10 that extends to a position. In the example shown in FIGS. 1 and 2, the connecting portion 29 extends linearly in the second direction d2.

Additionally, in the examples shown in FIGS. 1-4, the second end 242 of the container body 20 is closed. In the example shown in FIG. 2, the second end 242 is closed by a joint 22 formed by joining the film material 23.

The container main body 20 is configured such that the above-described extraction opening is formed closer to the second end 242 than the connecting portion 29 in the first direction d1.

Regarding the joint part 22 in the case where the facing parts of the film material 23 are peeled off from each other in a part of the joint part 22 to form an extraction opening closer to the second end part 242 than the connecting part 29 in the first direction d1 , will be further explained. A portion of the joint 22 located closer to the second end 242 than the connecting portion 29 in the first direction d1 is referred to as a first joint 22b, and a portion of the joint 22 other than the first joint 22b is referred to as a first joint 22b. 2 joint part 22c. In this case, as an example, the first joint portion 22b at least partially has a sealing strength of 3 N/15 mm or more and 6 N/15 mm or less. For example, the above-mentioned easily joined part 22a is formed as a part of the first joined part 22b, and the seal strength of the easily joined part 22a is 3 N/15 mm or more and 6 N/15 mm or less. This makes it easy to separate the facing portions of the film material 23 from each other to form an extraction opening in the portion of the first joint portion 22b that has the above-mentioned sealing strength. Therefore, the extraction opening can be formed closer to the second end portion 242 than the connecting portion 29 in the first direction d1. In the example shown in FIG. 2, the easy-to-join portion 22a is located at the second end 242. Here, the seal strength of the joint portion 22 is measured in accordance with JIS Z 0238:1998. As a measuring device, a tensile tester RTC-1310A with a thermostatic chamber manufactured by Orientech Co., Ltd. can be used.

The starting portion 26 will be further described in the case where the container main body 20 is cut by the above-mentioned starting portion 26 to form an extraction opening closer to the second end portion 242 than the connecting portion 29 in the first direction d1. In this case, the container body 20 has a starting portion 26 that is a starting position for cutting the container body, closer to the second end portion 242 than the connecting portion 29 in the first direction d1. In the example shown in FIG. 2, the start 26 includes a notch. Thereby, it is possible to cut the film material 23 using the starting portion 26 as the cutting starting position, and to form an extraction opening closer to the second end portion 242 than the connecting portion 29 in the first direction d1.

Further, the above-mentioned accommodating portion 21 is located closer to the first end portion 241 than the connecting portion 29 in the first direction d1. In the example shown in FIGS. 1 and 2, the connecting portion 29 has a first end portion side end portion 29b that is an end portion on the first end portion 241 side in the first direction d1. Furthermore, the connecting portion 29 has a second end portion side end portion 29c, which is an end portion on the second end portion 242 side in the first direction d1. In the example shown in FIGS. 1 and 2, the shapes of the first end side end 29b and the second end side end 29c are linear extending in the second direction d2. The above-mentioned accommodating portion 21 is located closer to the first end portion 241 than the first end portion side end portion 29b of the connecting portion 29 in the first direction d1.

Next, the expanded portion 40 will be explained. As shown in FIGS. 1 to 4, the flared portion 40 is connected to the connecting portion 29 of the container body 20. As shown in FIGS. Hereinafter, the portion of the expanding portion 40 that is connected to the container body 20 will also be referred to as the expanding connecting portion 41. In the illustrated example, the expanded portion 40 has a first expanded portion 40A and a second expanded portion 40B. As described above, the container body 20 has a pair of mutually opposing portions made of film material 23. A first widening portion 40A is attached to one of the pair of mutually opposing portions, and a second widening portion 40B is attached to the other of the pair of mutually opposing portions. When the container 10 immersed in the medium 51 is observed from the vertical direction, the expected extraction opening portion 16 is located between the first spread portion 40A and the second spread portion 40B.

The spreading part 40 has a spreading main body part 43 that spreads in a direction intersecting the first direction d1 when the container 10 is immersed in the medium 51, which is the liquid 511, from the first end 241 side of the container main body 20. . In the example shown in FIG. 4, the expanded main body portion 43 is expanded in a second direction d2 perpendicular to the first direction d1. In the example shown in FIG. 4, the flared portion 40 is manufactured as a separate member from the container body 20 and is attached to the container body 20. Although not shown, the container body 20 and the widening portion 40 may be integrally formed. For example, the expanding portion 40 may be a film that is integral with the film material 23 forming the container body 20 and is folded back to the film material 23 at the second end portion 242 . The material of the spreading part 40, especially the spreading main body part 43, is not particularly limited, but for example, a material having a specific gravity smaller than that of the medium 51 used for thawing the contained object C, typically a material having a specific gravity of less than 1, which is lighter than water. It is a material with specific gravity. Like the container body 20, the expanding portion 40 preferably has resistance to both high temperatures during thawing and low temperatures during freezing.

In the illustrated example, the expanded portion 40 has a resin film 45 connected to the connecting portion 29 of the container body 20. In the example shown in FIGS. 1 and 3, the film 45 is laminated on the outer surface of the container body 20. As the material of the film 45, various resin materials described as the material used for the container body 20 can be used. In particular, it is preferable that the film 45 be made of the same material as the container body 20 so that the film 45 can be stably joined to the container body 20 by thermal welding. Further, the thickness of the film 45 forming the widening portion 40 can be, for example, 1 μm or more and 2 mm or less. Note that the widening portion 40 may be a rigid member.

In the example shown in FIG. 3, the widening portion 40 has symmetry, particularly rotational symmetry, when observed from the first direction d1. When the widened portion 40 has rotational symmetry when observed from the first direction d1, the widened portion 40 has, for example, two-fold symmetry. The widening portion 40 may have line symmetry when observed from the first direction d1.

In the example shown in FIGS. 1 to 4, a container body 20 of the container 10 has a closed first end 241 and a second end 242, and an object C is stored inside the container body 20. is formed. The content C accommodated inside the container 10 is, for example, a biological material, specifically, a cell suspension containing cells cultured in a sterile state or cells before culture.

Next, a method of manufacturing the container 5 containing the stored material by accommodating the stored material C inside the container 10 will be described. As an example, a case will be described in which the content C is a cell suspension. FIG. 5 is a perspective view showing the container 10 before the contents C are stored therein. Moreover, FIG. 6 is a front view showing the container 10 before the contents C are stored therein. The accommodating portion 21 of the container 10 shown in FIGS. 5 and 6 is connected to the container body 20 through a portion of the container body 20 located closer to the second end portion 242 than the connecting portion 29 in the first direction d1. Leads to the outside world. The container body 20 of the container 10 shown in FIGS. 5 and 6 has an opening 28 on the second end 242 side with respect to the connecting portion 29 in the first direction d1, which allows the accommodating portion 21 to communicate with the outside. Therefore, the accommodating portion 21 communicates with the outside of the container body 20 via the opening 28 . The opening 28 is formed between the film materials 23 facing each other. The opening 28 shown in FIG. 5 is a portion that remains partially unjoined when the facing portions of the film material 23 are joined to form the joint 22. In this case, the container 10 containing the stored material is can be manufactured. In addition, as shown in FIG. 6, the container main body 20 may have the above-mentioned starting part 26 in the stage before the opening part 28 is sealed.

If the contained material C is a cell suspension, in the supply process, the contained material C is grade A in a sterile pharmaceutical manufacturing area where bacteria that harm cells are eliminated using an isolator, a cell culture processing center, etc. It is supplied to the storage unit 21 under the environment where it is classified. The mass of the contents C accommodated inside the container body 20 will be explained. FIG. 7 is a diagram showing the outline of the cross section of the container 10 when the container 10 is floated on the medium 51 as shown in FIG. 4, and also shows the range of the widened portion 40 and the like by hatching. As shown in FIG. 7, the volume of the expanding portion 40 (volume of the hatched portion) is V ₁ , including the inside of the portion of the container body 20 where the connecting portion 29 is located in the first direction d1. Let V ₂ be the total volume of the volume of the container 10 and the volume of the container 21 including the inside (volume of the hatched part), and let W ₁ be the mass of the container 10, and let the volume of the medium 51, which is the liquid 511, be V2. Let the density be ρ. In this case, the mass of the contained object C is less than or equal to the value of ρ(V ₁ +V ₂ )−W ₁ .

Next, in a sealing step, the opening 28 of the container body 20 is sealed in a closed environment. As an example, the opening 28 can be sealed by heat-sealing parts of the film material 23 that face each other at the opening 28 to form a joint 22 that closes the opening 28, as shown in FIG. will be stopped. The portions of the film material 23 that face each other at the opening 28 are joined such that the opening joint portion 22d formed by joining the film material 23 at the opening 28 has a sealing strength of 3 N/15 mm or more and 6 N/15 mm or less. You can. In this case, the opening joint portion 22d becomes the above-mentioned easily joined portion 22a. Then, it becomes easy to peel off the facing portions of the film material 23 from each other at the opening joint portion 22d to form a take-out opening. As a method for forming the opening joint portion 22d having the sealing strength within the above range, the method for forming the easily joined portion 22a described above can be used. In this way, the storage section 21 is maintained in a sterile and closed state, and the contents C containing cells are held within the storage section 21.

The method for manufacturing the container 5 containing the contents may further include a step of immersing the container 10 containing the contents C in a refrigerant such as liquid nitrogen or leaving it in a liquid nitrogen gas phase. Thereby, the contents C in the container 10 can be stored in a frozen state until used. In the following, the stored object C in a frozen state is also referred to as a frozen stored object.

Note that the container 10 has a widening portion 40 in addition to the container body 20 defining the storage portion 21 . In particular, the illustrated container 10 includes a first flared section 40A, which is a first floating member, and a second flared section 40B, which is a second floating member. However, the widening portion 40 is a film 45 disposed along the outer surface of the container body 20, and does not increase the size of the container 10. Therefore, the installation of the expanding portion 40 does not have a large effect on the storage of cells in the storage portion 21 of the container body 20, the storage of the container 10, and the like. Furthermore, it is possible to effectively prevent the spread portion 40 from interfering with the case when taking out the stored object C from the container 10.

In the container 5 containing contents, which is manufactured by the above method and includes the container 10 and the frozen contents located inside the container 10, 50% by mass or more of the frozen contents located inside the container 10 is preferably accommodated in the accommodating portion 21. Among the frozen stored items located inside the container 10, the proportion of frozen stored items stored in the storage section 21 is more preferably 70% by mass or more, and more preferably 90% by mass or more. preferable. It is better for the frozen stored items to be located inside the container 21 that contacts the medium 51 when the container 10 is floated on the medium 51 than to be located in a part of the inside of the container 10 other than the container 21. Due to heat exchange with the medium 51, it is thawed more quickly. Therefore, as described above, if the ratio of the frozen stored items stored in the storage section 21 is greater than a certain level, the frozen stored items can be thawed particularly quickly.

The frozen contents contained in the container 10 are thawed before use. A thawing method for thawing the frozen contents stored in the storage section 21 of the container 10 will be explained. The frozen contents stored in the container 10 include, for example, frozen cells, electrolyte infusions such as physiological saline, carbohydrate injections such as glucose, blood products, antibiotics, protein drugs such as antibodies, low molecular weight proteins, and hormones. Peptide drugs such as nucleic acid drugs, cell drugs, vaccines to prevent various infectious diseases, steroids, insulin, anticancer drugs, protease inhibitors, analgesics, antipyretic, analgesic and antiinflammatory drugs, anesthetics, fat emulsions, etc. Examples include antihypertensive agents, vasodilators, electrolyte correction injections such as heparin sodium chloride and potassium lactate, vitamin preparations, contrast agents, and suspensions containing liquids and fine particles.

As an example, a case will be described in which the frozen contents are frozen cells. Note that frozen cells are, for example, ones obtained by freezing a cell suspension. A cell suspension is, for example, a solution in which cells are suspended in a cell cryopreservation solution. When the frozen stored material is frozen cells, the thawing method for thawing the frozen stored material can also be said to be a method for manufacturing a cell preparation, in which a cell preparation is manufactured by thawing the frozen cells. In this case, cell preparations include hepatoma cells, liver parenchymal cells such as hepatocytes, Kupffer cells, endothelial cells such as vascular endothelial cells and corneal endothelial cells, fibroblasts, osteoblasts, osteoclasts, and periodontal ligament-derived cells. cells, epidermal cells such as epidermal keratinocytes, epithelial cells such as tracheal epithelial cells, gastrointestinal epithelial cells, cervical epithelial cells, corneal epithelial cells, mammary gland cells, pericytes, muscle cells such as smooth muscle cells and cardiomyocytes , kidney cells, pancreatic islet cells, nerve cells such as peripheral nerve cells and optic nerve cells, chondrocytes, bone cells, stem cells, ES cells (embryonic stem cells), iPS cells (induced pluripotent stem cells), etc. Ru. Examples of stem cells include bone marrow undifferentiated mesenchymal stem cells, hematopoietic stem cells, vascular stem cells, neural stem cells, small intestine stem cells, adipose stem cells, skin stem cells, periodontal tissue stem cells, ciliary body stem cells, corneal limbal stem cells, visceral stem cells, etc. It will be done. The frozen contents stored in the storage section 21 are thawed by heat exchange with the medium 51, which is a liquid 511.

The method for thawing frozen stored items includes a thawing step in which the container 10 is immersed in the medium 51, which is the liquid 511, from the first end 241 side of the container body 20. At this time, the container 10 obtains buoyancy by sinking a portion of the container body 20 and at least a portion of the widening portion 40 below the interface 51a of the medium 51, and the second end portion 242 of the container body 20 gains buoyancy. It floats on the medium 51 with its face facing upward. Here, as shown in FIG. 4, when the container 10 is immersed in the medium 51 held in the medium holding container 50 from the first end 241 side of the container main body 20, the storage part 24 of the container main body 20 is immersed in the medium 51, and floats on the medium 51 in a state in which the expected extraction opening portion 16 of the container body 20 is not immersed in the medium 51. For this reason, it is possible to prevent the medium 51 from adhering to the scheduled take-out opening portion 16 while thawing the frozen contents stored in the storage portion 21 through heat exchange with the medium 51 . Thereby, when a take-out opening is formed in the scheduled take-out opening portion 16 and the stored object C is taken out through the take-out opening, it is possible to prevent the stored object C from being contaminated by the medium 51.

The medium 51 is not particularly limited as long as it can thaw the frozen contents, and is, for example, a liquid 511, particularly water. The temperature of the medium 51 is not particularly limited as long as it can thaw the frozen contents. As an example, when the frozen contents are frozen cells and a cell preparation is produced by thawing the frozen cells, the temperature of the medium 51 is, for example, 30°C or more and 40°C or less, typically 37°C. It is.

As shown in FIG. 7, the volume of the expanding portion 40 is V ₁ , the volume of the container body 20 including the inside of the portion where the connecting portion 29 is located in the first direction d1, and the volume of the accommodating portion 21 including the inside thereof. Let V ₂ be the total volume and W ₁ be the mass of the container 10. Further, the density of the medium 51, which is the liquid 511, is assumed to be ρ. In this case, it is preferable that the following equation (4) holds true.

Note that when the medium 51 is water, it is preferable that the following equation (1) holds true, assuming that the density of water is ρ _W. Note that the density of water ρ _W is the density of water at, for example, 37° C., and is, for example, 1 g/cm ³ .

The effect of formula (4) being established will be explained. Suppose that the container 10 is immersed in the medium 51, which is the liquid 511, up to the end of the expanding portion 40 on the second end 242 side in the first direction d1, that is, to the position of the dashed-dotted line L4 shown in FIG. think about. In this case, the value of V ₁ +V ₂ corresponds to the volume of the portion of the volume of the container 10 including the inside that is located below the interface 51a of the medium 51. The buoyant force acting on the container 10 immersed in the medium 51 up to the position indicated by the dashed-dotted line L4 is expressed as ρ(V ₁ +V ₂ )g, where g is the gravitational acceleration. Assuming that the container 10 does not contain the contents C inside, the gravitational force acting on the container 10 is represented by W ₁ g. Here, if equation (4) holds true, the buoyant force ρ(V ₁ +V ₂ )g in the state of being immersed in the medium 51 up to the position of the dashed-dotted line L4 becomes larger than the gravity W ₁ g. Therefore, when the container 10 does not contain the contents C therein, the container 10 floats in a state where the portion closer to the second end portion 242 than the expanding portion 40 is not immersed in the medium 51.

Furthermore, a case will be considered in which the container 10 accommodates the contents C therein to form the container 5 containing the contents. In this case, if the mass of the object C accommodated inside the container 10 is less than or equal to the value of ρ(V ₁ +V ₂ ) - W ₁ , the object-containing container 5 is immersed in the medium 51 up to the position indicated by the dashed-dotted line L4. The buoyant force acting on the container 5 becomes greater than the gravitational force acting on the container 5 containing the contents. Therefore, the container 5 containing the contents floats in a state where the portion closer to the second end 242 than the widening portion 40 is not immersed in the medium 51. From the above, when formula (4) is established, when the mass of the contained object C accommodated inside the container 10 is set to be less than or equal to the value of ρ(V ₁ +V ₂ )−W ₁ The adhesion of the medium 51 can be more effectively suppressed.

Next, the effects of the expanding portion 40 when the container 10 is floated on the medium 51 as shown in FIG. 4 will be explained. First, let us consider a case where, as shown in FIG. 8, a general container 10' having a cylindrical shape is floated on a medium 51 and the container 10' is tilted. In the example shown in FIG. 8, the center of buoyancy B of the container 10' is located on the side where the container 10' is inclined (the right side in FIG. 8) with respect to the center of gravity G of the container 10'. In this case, a force that attempts to eliminate the inclination of the container 10', that is, a restoring force, acts on the container 10' depending on the positional relationship between the center of gravity G and the center of buoyancy B.

Hereinafter, the intersection M between the axis of symmetry of the container 10', particularly L1, which is the axis of rotational symmetry of the container 10' having a cylindrical shape, and the line of action L3 of the buoyant force P2 acting on the container 10' is also referred to as metacenter M. . In other words, whether a restoring force acts on the container 10' is determined by the positional relationship between the center of gravity G and the metacenter M. In the example shown in FIG. 8, since the center of buoyancy B is located on the side where the container 10' is inclined relative to the center of gravity G, the line of action L3 of the buoyant force P passing through the center of buoyancy B and the axis of symmetry L1 intersect. A certain metacenter M is located above the center of gravity G. If the metacenter M is above the center of gravity G as in the example of FIG. 8, a moment of force is generated that eliminates the inclination of the container 10'.

The restoring force acts to rotate the container 10' due to the downward gravity P1 and the upward buoyant force P2 acting on the container 10'. The magnitude P of the restoring force can be expressed by the following equation (5), where w2 is the distance between the line of action L2 of gravity and the line of action L3 of buoyancy shown in FIG. 8, and m is the mass of the container 10'. .

Furthermore, if the container 10' floating on the medium 51 is tilted as shown in FIG. 9, the center of gravity G is on the side where the container 10' is tilted (the right side in FIG. 9) than the center of buoyancy B. Also consider what happens if you move to . In this case, as shown in FIG. 9, the metacenter M, which is the intersection of the line of action L3 of the buoyant force P passing through the center of buoyancy B and the axis of symmetry L1, is located below the center of gravity G. When the metacenter M is located below the center of gravity G as in the example of FIG. 9, a moment of force is generated that further tilts the container 10', making it impossible to apply a restoring force to the container 10'.

From FIGS. 8 and 9 and equation (5), in order to stably exert a sufficient amount of restoring force on the container, the shape of the container must be such that when the container is tilted, the center of buoyancy B is It can be understood that a shape that allows the container to move more easily to the tilted side than the center of gravity G is preferable. Further, in order to make the shape of the container such that the center of gravity G is difficult to move, it is preferable that the center of gravity G of the container is located at a low position.

Next, let us consider a case where the container 10 according to the present embodiment is tilted to one side as shown in FIG. 10 from a state where it is floating on the medium 51 as shown in FIG. In Figure 10, the container 10 is tilted to the left in the figure. In this case, on the side where the container 10 is tilted, the portion of the expanded portion 40 that was located above the interface 51a of the medium 51 in FIG. 10 sinks below the interface 51a. Furthermore, on the side opposite to the side on which the container 10 is tilted, the portion of the spread portion 40 that was located below the interface 51a of the medium 51 in FIG. 10 rises above the interface 51a. As a result, of the volume of the container 10 including the inside, the volume of the portion located below the interface 51a of the medium 51 increases greatly on the side where the container 10 is tilted, and on the side opposite to the side where the container 10 is tilted. There is a large decrease in In the example shown in FIG. 10, the volume of the portion of the medium 51 located below the interface 51a increases greatly on the left side when the container 10 is tilted, and decreases greatly on the right side, which is the opposite side to the side where the container 10 is tilted. are doing. Therefore, the center of buoyancy of the container 10 moves significantly toward the side where the container 10 is tilted compared to before the container 10 is tilted. Thereby, when the container 10 is tilted to one side, the center of buoyancy can be largely moved to the tilted side, and a restoring force can be stably exerted on the container 10. This makes it possible to more stably maintain the state in which the container 10 is floating above the medium 51 so that the expected extraction opening portion 16 of the container body 20 is not immersed in the medium 51.

Furthermore, the widening portion 40 has symmetry, particularly rotational symmetry, when observed from the first direction d1. Since the widening portion 40 has the above-described shape, when the container 10 is floating on the medium 51 as shown in FIG. 4, the second end 242 of the container body 20 is directed upward. can be made more stable. Further, when the container 10 is floating on the medium 51 as shown in FIG. becomes easier to work with.

Furthermore, in the specific example described above, the ratio X (%) expressed by the following formula (2) is preferably 0.3% or more. By setting the ratio X (%) within the above range, the volume V ₁ of the expanding portion 40, the volume including the inside of the portion of the container body 20 where the connecting portion 29 is located in the first direction d1, and the inside The ratio of the volume V ₁ of the spread portion 40 to the sum of the volume of the storage portion 21 including the total volume V ₂ can be made sufficiently large. For this reason, when the container 10 floating on the medium 51 is tilted, the action of the widening portion 40 of moving the center of buoyancy of the container 10 by a large amount and stably exerting a restoring force on the container 10 is increased. It can be done quickly.

In addition, in the above-described specific example, the value obtained by dividing the volume V ₁ of the widened portion 40 by the area of the connecting surface 29a forming the boundary between the widened portion 40 and the connecting portion 29 is 0.15 (mm ³ /mm ² ) or more. Thereby, the volume V ₁ of the widened portion 40 can be made sufficiently large in proportion to the area of the connecting surface 29a. For this reason, when the container 10 floating on the medium 51 is tilted, the action of the widening portion 40 of moving the center of buoyancy of the container 10 by a large amount and stably exerting a restoring force on the container 10 is increased. It can be done quickly.

In addition, in one specific example described above, when the container 10 is immersed in the medium 51 which is the liquid 511 from the first end 241 side of the container main body 20, the widening portion 40 is a portion of the container main body 20 other than the connecting portion 29. protrude away from As a result, the widening part 40 and the accommodating part 21 of the container body 20 are separated, so that the accommodating part 21 and the medium 51 are brought into contact with each other as shown in FIG. 51 can be efficiently generated.

Furthermore, in the specific example described above, it is preferable that the accommodating section 21 is configured so that the efficiency of heat exchange between the stored object C accommodated in the accommodating section 21 and the medium 51 is increased. For example, the thickness of the film material 23 in the housing portion 21 is preferably 1 mm or less. The thickness of the film material 23 is more preferably 0.5 mm or less, more preferably 0.26 mm or less. Moreover, it is preferable that the thermal conductivity of the film material 23 in the housing part 21 is 0.1 W/m·K or more. Thereby, the frozen contents can be thawed more quickly by heat exchange with the medium 51.

Furthermore, in the specific example described above, it is preferable that the surface area of the accommodating portion 21 is large. Since the surface area of the storage part 21 is large, the contact area between the storage part 21 and the medium 51 can be increased, and the efficiency of heat exchange between the stored object C and the medium 51 can be increased. This allows the frozen contents to be thawed more quickly. As an example, a dimension t3 of the accommodating part 21 in the first direction d1 shown in FIG. 2 is larger than a dimension t4 of the accommodating part 21 in the second direction d2 perpendicular to the first direction d1. In the specific example described above, the dimension t4 corresponds to the dimensions of the first side 20a and the second side 20b. The dimension t3 is more preferably 1.1 times or more, more preferably 1.3 times or more, the dimension t4. By determining the relationship between the dimension t3 and the dimension t4 as described above, the surface area of the accommodating part 21 on the side surface can be increased and the surface area of the accommodating part 21 can be made sufficiently large.

According to the container 10 having the storage part 21 that can increase the efficiency of heat exchange between the contents C and the medium 51, for example, the frozen contents C of 1000 ml or less can be thawed within 8 minutes. obtain. Moreover, the content C of 15 ml or less can be thawed within 3 minutes.

It is preferable that the accommodating portion 21 has rotational symmetry in a cross section perpendicular to the first direction d1. The housing portion 21 according to the present embodiment has a shape that is two-fold symmetrical in a cross section perpendicular to the first direction d1. By having the above-described shape of the accommodating portion 21, when the container 10 is floating on the medium 51 as shown in FIG. can be made more stable. In addition, it is possible to suppress the formation of a portion that is particularly far away from the medium 51 inside the accommodating portion 21, so that the heat from the medium 51 can be more evenly transmitted to the stored object C.

Further, the connecting portion 29 is preferably located between the center of gravity of the container body 20 and the expected extraction opening portion 16 in the first direction d1. Thereby, the position of the center of gravity of the container 10 can be set at a sufficiently low position (position sufficiently close to the first end 241 side). For this reason, when the container 10 floating on the medium 51 is tilted, the center of gravity of the container 10 becomes more difficult to move, and the medium 51 can be stored in a stable manner, especially in a state where the planned extraction opening section 16 is not immersed in the medium 51. 51.

After the frozen contents have been thawed, the contents C inside the container 10 are put to use in the following manner. First, the container 10 is pulled up from the medium 51. Thereafter, a take-out opening for taking out the stored object C is formed in the scheduled take-out opening portion 16 . Next, the thawed contents C in the container 10 are taken out from the take-out opening. Then, the taken-out stored object C is put to use.

In addition, in JP2018-110539A and JP2001-70402A, which were introduced as conventional techniques, in order to suppress the adhesion of liquid to the planned extraction opening of the container, the container is covered with the exterior before thawing the contents. It is stored in a bag. After the contents are thawed by immersing the outer bag in liquid, the container is removed from the outer bag. In comparison with such conventional technology, according to the present embodiment, the work of storing the container 10 containing the frozen stored object in the outer bag before the thawing operation, and the operation of storing the thawed stored object C into the container 10 are performed. It becomes possible to omit the work of taking out the container 10 from the outer bag before taking it out from the take-out opening. Thereby, it is possible to significantly shorten the preparation time and reduce the preparation work until the frozen stored material C is used. Such effects are particularly suitable when applying thawed biological materials, such as cells such as cell suspensions and cell sheets, to the human body.

Furthermore, the widening portion 40 can exhibit a protective function for the container body 20. For example, if the frozen container 5 is dropped, the container 5 may collide with a floor, a desk, a processing table, etc., and may receive an impact from the floor, desk, processing table, etc. In such a case, the widening portion 40 receives the impact preferentially than the container main body 20, and the impact to the container main body 20 can be prevented.

In addition, in the above-mentioned specific example, an example was shown in which one widening part 40 is provided on one main surface of the container body 20, but two or more spreading parts 40 are provided on one main surface. Good too. For example, two or more spread portions 40 may be attached to the container body 20 via different spread connection portions 41 on one main surface. Further, the plurality of spread parts 40 may be connected to the container body 20 by overlapping the spread connection part 41 of one spread part 40 and the spread connection part 41 of another spread part 40. According to such an example, when the expanding portion 40 is the floating member 401, the buoyancy of the container 10 can be further strengthened. Further, the protection function of the widened portion 40 can also be improved.

Further, as described above, the expanding portion 40 may be a floating member 401 formed of a material having a smaller specific gravity than the medium 51 used for thawing the stored object C. Floating member 401 is typically made of a material that is lighter than water and has a specific gravity of less than 1. In this case, the floating member 401 floats above the medium 51 with the container body 20 at least partially submerged within the medium 51. The floating member 401 has a take-out opening or a planned take-out opening that allows the storage part 21 to communicate with the outside when taking out the stored object C from the storage part 21 while the container body 20 is at least partially immersed in the medium 51. portion 16 is located outside the medium 51.

In order to effectively prevent the medium 51 from adhering to the take-out opening during thawing of the contents C, the floating member 401 is connected to the container body 20 in the vicinity of the take-out opening or the scheduled take-out opening 16. It is preferable that Preferably, as shown in FIGS. 3 and 4, the connection position of the floating member 401 to the container body 20 is between the take-out opening or the scheduled take-out opening 16 and the frozen contents C in the storage part 21. positioned. Further, when the container 10 has a plurality of floating members 401 and the container 10 immersed in the medium 51 is observed from the vertical direction, the take-out opening portion or the planned take-out opening portion 16 is between the plurality of floating members 401. Preferably located.

In the illustrated example, the floating member 401 has a resin film 45 connected to the container body 20. A floating member 401 made of a film 45 has a flared connection portion 41 as a part thereof. The floating member 401 is joined to the container body 20 at the flared connection portion 41 . The spread connection portion 41 may be joined to the container body 20 by thermal welding, or may be joined to the container body 20 via a bonding layer made of adhesive or adhesive. The spread connection portion 41 is formed by a linear, especially linearly extending edge of the resin film 45. The expanding connection portion 41 extends along the extraction opening or the planned extraction opening portion 16 . Further, the expanding connecting portion 41 is located between the take-out opening or the planned take-out opening portion 16 and the frozen stored object C in the storage portion 21 . More precisely, the expanding connection part 41 is between the take-out opening or scheduled take-out opening part 16 and the stored object C in the direction along the straight line connecting the take-out opening or planned take-out opening part 16 and the stored object C. It is located in Further, the expanding connection part 41 is located between the take-out opening or the intended take-out opening 16 and the center of gravity of the container body 20. The center of gravity of the container body 20 used in this specification refers to the center of gravity of the container containing the contents C immediately before being thawed, that is, the center of gravity of the container 5 containing the contents immediately before heat exchange with the medium 51. In particular, the widening connecting portion 41 connects the take-out opening or planned take-out opening 16 and the center of gravity of the container body 20 in the direction along the straight line connecting the take-out opening or scheduled take-out opening 16 and the center of gravity of the container body 20 . It is located in between.

The floating member 401 further includes an extension portion 42 extending from the flared connection portion 41 . Here, the extending portion 42 refers to the expanding main body portion 43 when the expanding portion 40 corresponds to the floating member 401, for example. The extending portion 42 extends along the outer surface of the container body 20. In particular, the extending portion 42 extends from the connecting portion 41 toward the side away from the take-out opening or expected take-out opening portion 16 . Film 45 can be bent by curving or bending. As shown in FIG. 4, the film 45 can be separated from the container main body 20 at the extending portion 42 by bending.

The film 45 forming the floating member 401 is made of a material having a smaller specific gravity than the medium 51, as described above. Therefore, when the container 10 is immersed in the medium 51, the floating member 401 made of the film 45 floats on the medium 51. Moreover, it is preferable that the film 45 forming the floating member 401 has flexibility or flexibility. In order to impart appropriate elasticity to the floating member 401 as described later, the thickness of the film 45 forming the floating member 401 may be made thicker than the thickness of the film material 23 forming the container body 20.

Next, the operation of the container 10 provided with the above floating member 401 will be explained.

As shown in FIG. 4, just before using the biological material, the container 10 is immersed in a medium 51 held in a medium holding container 50. At this time, the container 10 is immersed in the medium 51 from the side away from the take-out opening or the scheduled take-out opening 16. Here, when the spreading part 40 is the floating member 401, the first spreading part 40A and the second spreading part 40B attached to the pair of opposing parts of the film material 23 forming the container body 20 are smaller than the medium 51. The film 45 has a specific gravity. This film 45 is attached to the container body 20 at the edge on the side close to the take-out opening or planned take-out opening 16. Thus, as shown in FIG. 4, the film 45 curves away from the container body 20 and floats on the surface of the medium 51. That is, the film 45 forming the first spreading part 40A and the film 45 forming the second spreading part 40B extend away from the container body 20 and spread on the surface of the medium 51.

The expanding connection portion 41 of the floating member 401 connected to the container body 20 is located near the take-out opening or the planned take-out opening portion 16. In addition, the spreading connection part 41 of the floating member 401 is a take-out opening that allows the storage part 21 to communicate with the outside of the container main body 20 when taking out the stored item C stored in the storage part 21 and the stored item C from the storage part 21. or the scheduled extraction opening portion 16. Furthermore, the expanding connection portion 41 of the floating member 401 is located between the center of gravity of the container body 20 and the take-out opening or the planned take-out opening portion 16 . Therefore, the extraction opening or the planned extraction opening 16 is maintained above the surface of the medium 51 in the vertical direction, as shown in FIG. In other words, the medium 51 is effectively prevented from adhering to the take-out opening.

In the illustrated example, since the film 45 is used as the floating member 401, not only the buoyant force due to the specific gravity of the film 45 but also the elastic force trying to resist the curvature of the floating member 401 is applied to the take-out opening or the scheduled take-out opening. It acts to float the section 16 from the medium 51. Additionally, film 45 extends over the surface of media 51. In particular, the floating member 401 has a pair of films 45 extending on both sides in a direction perpendicular to the flat container body 20, as a first spread part 40A and a second spread part 40B. With each of these configurations, the attitude of the container 10 floating on the surface of the medium 51 is stabilized, and the take-out opening or scheduled take-out opening portion 16 is held on the surface of the medium 51 so that the medium 51 to the take-out opening is stabilized. can effectively prevent the adhesion of

According to the container 10 in which the expanding portion 40 is the floating member 401 as described above, the container 10 used when thawing the contained material C containing biological materials such as cells by contact with the medium 51 can be removed. The container body 20 includes a container body 20 having a housing section 21 that communicates with the outside through an opening, and a floating member 401 connected to the outer surface of a portion of the container body 20 that forms the housing section 21. The material used for floating member 401 has a lower specific gravity than medium 51. Therefore, when the container 10 is immersed in the medium 51 to thaw the content C containing biological material, the floating member 401 facilitates positioning of the extraction opening or the intended extraction opening 16 outside the medium 51. . Therefore, it is possible to effectively prevent the medium 51 from adhering to the take-out opening and from contaminating the take-out opening with the medium 51.

Furthermore, the floating member 401 is connected to the container body 20 in the vicinity of a take-out opening or a planned take-out opening portion 16 that allows the storing portion 21 to communicate with the outside when taking out the stored object C from the storing portion 21 . According to such a floating member 401, when the container 10 is immersed in the medium 51, it is possible to effectively prevent the take-out opening or the planned take-out opening portion 16 from being submerged in the medium 51.

In one specific example of the embodiment described above, the floating member 401 has a resin film 45 that is provided on the outer surface of the container body 20 and is connected to the container body 20 only at the expanded connection portion 41 that is a part thereof. ing. This resin film 45 is spaced apart from the spreading connection part 41 connected to the container body 20 and the take-out opening or expected take-out opening part 16 that communicates the storing part 21 to the outside when taking out the stored object C from the storing part 21. It has an extension part 42 that widens to the side and extends from the connection part 41. The extending portion 42 can be separated from the container body 20 by bending. According to such a floating member 401, due to the buoyancy caused by the specific gravity of the resin film 45 and the elastic force of the resin film 45, the take-out opening or the scheduled take-out opening 16 is moved above the medium 51. It can be positioned stably. Thereby, when the container 10 is immersed in the medium 51, it is possible to more effectively prevent the take-out opening portion or the planned take-out opening portion 16 from being immersed in the medium 51. Further, since the floating member 401 is a resin film 45 connected to the container body 20, it is possible to effectively prevent the container 10 from increasing in size and weight. Furthermore, it is possible to effectively prevent the floating member 401 from interfering with the removal of the contents C from the container 10.

In one specific example of the embodiment described above, the spread connection portion 41 is a linearly extending edge of the resin film 45. Thereby, it is possible to effectively prevent the resin film 45 from interfering with the removal of the contents C from the container 10.

In one specific example of the embodiment described above, the container main body 20 is made of two resin film materials 23 that are overlapped and joined at their facing parts, or a resin film material 23 that is folded back and joined at their facing parts. It has a film material 23. The floating member 401 includes a first spread part 40A and a second spread part 40B provided at a pair of opposing parts of a resin film material, respectively. According to such a floating member 401, the attitude of the container 10 can be stabilized when the container 10 is immersed in the medium 51. In particular, it is suitable for stabilizing the posture of the container 10 having a flat shape. Thereby, it is possible to more effectively prevent the take-out opening portion or the planned take-out opening portion 16 from being immersed in the medium 51.

Although one embodiment has been described using a plurality of specific examples, these specific examples are not intended to limit the one embodiment. The embodiment described above can be implemented in various other specific examples, and various omissions, substitutions, changes, and additions can be made without departing from the gist thereof.

Hereinafter, an example of the modification will be described with reference to the drawings. In the following explanation and the drawings used in the following explanation, the same reference numerals as those used for corresponding parts in the above specific example are used for parts that may be configured in the same way as in the above specific example, and redundant explanations are used. omitted.

First, in the specific example described above, the resin film 45 forming the widening part 40 was arranged so as to spread over the outer surface of the container body 20, and one edge thereof was joined to the container body 20. However, as shown in FIGS. 11 to 13, the resin film 45 is folded back and the overlapped portion of the folded film 45 is spread out and joined to the container body 20 as a connection part 41. Good too. In the example shown in FIGS. 11 to 13, the film 45 constituting the widening portion 40 is a rectangular film, and is bent at an intermediate portion in the longitudinal direction so as to be folded back about an axis perpendicular to the longitudinal direction. It is being Then, the film 45 that is bent back is placed on the outer surface of the container body 20 and fixed to the container body 20. In this example, a pair of edges in the longitudinal direction of the rectangular film 45, each extending in the lateral direction, are overlapped to form a widening connection part 41 that is joined to the container body 20. There is. Other configurations, such as the position of the flared connection portion 41 on the container body 20, can be the same as in the above-described specific example described with reference to FIGS. 1 to 4.

Also in this modification, as shown in FIG. 13, the spread portion 40 can prevent the medium 51 from adhering to the scheduled extraction opening portion 16. In addition, when the widening portion 40 is a floating member 401, the rigidity of the floating member 401 increases, and the floating member 401 pushes the surface of the medium 51 and tries to lift the take-out opening part or the planned take-out opening part 16 of the container 10. Elastic force can be enhanced. Therefore, when the container 10 is immersed in the medium 51 to thaw the content C containing biological material, the extraction opening or the planned extraction opening 16 can be stably held above the medium 51. Thereby, it is possible to effectively prevent the medium 51 from adhering to the take-out opening and from contaminating the take-out opening with the medium 51.

Further, as shown in FIGS. 14 and 15, the container 10 may include a cylindrical member 30 connected to the container body 20 so as to communicate with the storage portion 21. In the illustrated example, the cylindrical member 30 is connected to the second end 242 side of the container body 20. In the illustrated example, two cylindrical members 30 are provided in the container body 20 as separate bodies from the container body 20. The cylindrical members 30 pass through the joint 22, and one end of each cylindrical member 30 is located inside the container body 20. However, the cylindrical member 30 may be integrally formed with the container body 20. Alternatively, the end portion of the cylindrical member 30 may be held by the joint portion 22, and the cylindrical member 30 may open into the accommodating portion 21 at the end portion held by the joint portion 22. Furthermore, the number of cylindrical members 30 held in one container 10 may be one, or may be three or more. In an example other than the illustrated example, the number of cylindrical members 30 held in one container 10 is three. According to the container 10 having the cylindrical member 30, it is possible to aseptically connect it to other containers using the cylindrical member 30 to construct a closed environment, for example, a cell culture system. That is, while effectively reducing contamination from the outside (outside air), it can be aseptically connected to various processing containers such as cell storage containers, culture solution storage containers, culture containers, and drainage containers.

For example, when the container 10 is not connected to another container etc. via the cylindrical member 30, the outer end 31 of the ends of the cylindrical member 30 located outside the container body 20 is closed. There is. In other words, the end of the cylindrical member 30 on the side away from the container body 20 is sealed. By sealing the end of the container body 20, the accommodating portion 21 of the container body 20 can be maintained in a closed state. On the other hand, in use, the cylindrical member 30 can be aseptically connected to a tube material or the like leading to another container or the like using, for example, an aseptic connecting device. For example, the outer end 31 is closed by a seal formed by joining the opposing sides of the cylindrical member 30. In this case, the cylindrical member 30 is preferably made of a material with a low melting temperature and high flexibility so that the ends of the cylindrical member 30 can be easily sealed by heat sealing. As specific examples, polyolefin resins such as polyethylene and polypropylene, polyvinyl chloride, ethylene vinyl acetate copolymer resins, and thermoplastic elastomers (C-FLEX, etc.) can be used as the material for the cylindrical member 30.

Further, when the outer end 31 is closed, the container 10 may further include a lid member that closes the outer end 31, although not shown. In one example, the lid member is a member that is detachable from the outer end portion 31 and has a cap shape. Further, the lid member may be a member adhered to the outer end 31 so that it can be peeled off from the outer end 31. Further, the lid member may be a member made of a material such as a glass tube that is easy to puncture, such as rubber.

Further, when the outer end 31 is closed, the cylindrical member 30 may have a thin wall portion that closes the outer end 31, although not shown. The thin portion is configured to facilitate puncture with a syringe or a syringe needle. The thickness of the thin portion is preferably 1 mm or less, more preferably 0.5 mm or less, and even more preferably 0.3 mm or less.

As an example, a sterile connection port may be provided at the end of the cylindrical member 30 on the side away from the container 10, so that it can be sterilely connected to a tube material or the like leading to another container or the like during use. The sterile connection port may have multiple connections to allow connection to multiple instruments. Alternatively, the extraction opening scheduled portion 16 may be formed by providing a port cover (not shown) as a lid material so as to cover the opening provided at the tip of the cylindrical member 30, in which case, The opening opened by removing the port cover is used as an ejection opening, and a syringe with a needle is connected to the ejection opening to take out the contained substance C or inject a substance (for example, a diluent) into the storage part 21. Good too. The syringe with a needle may be connected directly to the extraction opening, or the syringe with a needle may be connected directly to the extraction opening via a connector (for example, an adapter used for an infusion set, etc.) that connects the extraction opening and the syringe with an injection needle. In the latter case, a connecting device is connected to the extraction opening.

In the example shown in FIGS. 14 and 15, the cylindrical member 30 forms a take-out opening that allows the storage portion 21 to communicate with the outside when the stored object C is taken out from the storage portion 21. In this example as well, the spread portion 40 can prevent the medium 51 from adhering to the scheduled extraction opening portion 16 . Further, in this example, the widening portion 40 is a floating member 401, and is connected to the container body 20 in the vicinity of the position where the cylindrical member 30 is connected to the container body 20. More precisely, the cylindrical member 30 is connected to the joint 22 forming one edge of the container body 20, and the widening part 40, which is the floating member 401, is connected to the joint 22 near the joint 22 to which the cylindrical member 30 is connected. It is connected to the container body 20 at. Further, the widening connection portion 41 of the floating member 401 extends linearly along the longitudinal direction of the joint portion 22 to which the cylindrical member 30 is connected. Even in such an example, as shown in FIG. 15, when the container 10 is immersed in the medium 51, it is possible to effectively prevent the extraction opening or the planned extraction opening 16 from being submerged in the medium 51. I can do it.

A method for storing the contents C inside the container 10 shown in FIGS. 14 and 15 will be described. FIG. 16 is a perspective view showing the container 10 including the cylindrical member 30 before the contents C are stored therein. In the example shown in FIG. 16, the outer end 31 of the cylindrical member 30 is open. First, a supply step is performed in which the contents C are supplied to the storage section 21 through the open cylindrical member 30 of the container 10 . Next, a sealing step for sealing the outer end portion 31 is performed. Thereby, the contents C can be accommodated inside the container 10.

Although not shown, the container body 20 also includes a first fitting portion that is provided on the inner surface of the film material 23 closer to the second end portion 242 than the connecting portion 29 in the first direction d1, and that is capable of fitting into each other. It may have a second fitting part. As an example, the first fitting portion is provided on the inner surface of the first surface 23d of the film material 23, and extends in the second direction d2 from the third side 20c to the fourth side 20d. Further, the second fitting portion is provided on the inner surface of the second surface 23e of the film material 23, and extends in the second direction d2 from the third side 20c to the fourth side 20d. In this case, the second end portion 242 is closed by fitting the first fitting portion and the second fitting portion. Moreover, by unfitting the first fitting part and the second fitting part, the accommodating part 21 is moved to the outside of the container body 20 through the space between the first fitting part and the second fitting part. It gets through. Note that the expression that the accommodating part 21 communicates with the outside of the container main body 20 refers to that the accommodating space inside the accommodating part 21 communicates with the outside of the container main body 20.

As an example, the container body 20 has a zipper tape extending in the second direction d2 from the third side 20c to the fourth side 20d, and includes a first fitting part and a second fitting part as part of the zipper tape. have. In this case, a portion of the film material 23 of the zipper tape provided on the inner surface of the first surface 23d corresponds to the first fitting portion. Further, in the zipper tape, a portion of the film material 23 provided on the inner surface of the second surface 23e corresponds to a second fitting portion. The zipper tape may be a so-called slide chuck type zipper tape. In this case, the zipper tape fits the first fitting part and the second fitting part by sliding in the direction in which the first fitting part and the second fitting part extend, or the first fitting part The second fitting part further includes a slider for removing the fitting between the second fitting part and the second fitting part.

Further, the widening portion 40 has a widening main body portion 43 that expands in a direction intersecting the first direction d1 when the container 10 is immersed in the medium 51, which is the liquid 511, from the first end 241 side of the container body 20, and It may have a spreading assisting part 46 connected to the end of the spreading main body part 43 on the side away from the container main body 20. FIG. 17 is a diagram showing a state in which the container 10 in a modified example in which the spreading part 40 has the spreading assisting part 46 is floated on the medium 51. As shown in FIG. 17, when the container 10 is immersed in the medium 51 from the first end 241 side of the container body 20, the spreading auxiliary part 46 is connected to the first end of the spreading main body 43 in the first direction d1. 241 side, and extends in the first direction d1.

The spreading auxiliary part 46 may be integrally molded with the spreading main body part 43 or may be manufactured as a separate member from the spreading main body part 43 and attached to the spreading main body part 43. As the material for the expansion assisting portion 46, the same material as the material for the expansion main body portion 43 described above can be used. When the spreading assisting part 46 is manufactured as a separate member from the spreading main body part 43, the spreading assisting part 46 may or may not have rigidity.

To explain the effects of the container 10 shown in FIG. 17, consider a case where the container 10 floating on the medium 51 is tilted to one side (for example, to the left in FIG. 17). In this case, in the container 10 having the expansion assisting portion 46, the center of buoyancy tends to move more toward the tilted side of the container 10 than in the container 10 shown in FIGS. 1 to 4. For this reason, when the container 10 floating on the medium 51 is tilted, the action of the widening portion 40 of moving the center of buoyancy of the container 10 by a large amount and stably exerting a restoring force on the container 10 is increased. It can be done quickly. When the container 10 is tilted due to waves generated in the medium 51 due to the restoring force, the tilt of the container 10 is canceled. In addition, even if the inclination is not eliminated due to the restoring force, the container 10 is placed above the medium 51 while maintaining a certain inclination so that the planned extraction opening portion 16 does not submerge in the medium 51. Sometimes it keeps floating. Also in this case, adhesion of the medium 51 to the scheduled extraction opening portion 16 can be suppressed.

FIG. 18 is a diagram showing the outline of the cross section of the container 10 when the container 10 is floated on the medium 51 as shown in FIG. 17, and also shows the range of the widened portion 40 and the like by hatching. As shown in FIG. 18, the volume of the widening portion 40 (the total volume of the diagonally hatched part and the horizontally hatched part) is V ₁ , and the volume of the expanding part 40 is V 1 , The total volume of the volume including the inside of the part where the connecting part 29 is located and the volume of the accommodating part 21 including the inside (volume of the part hatched with dots) is V ₂ , and the expansion auxiliary part 46 Let the volume of (volume of the portion hatched with horizontal lines) be _V3 . In this case, it is preferable that the ratio Y (%) expressed by the following formula (3) is 0.2% or more. By setting the ratio Y (%) within the above range, the volume V ₁ of the expanding portion 40, the volume including the inside of the portion of the container body 20 where the connecting portion 29 is located in the first direction d1, and the inside The ratio of the volume _V3 of the expansion assisting part 46 to the sum of the volume of the storage part 21 including the total volume _V2 can be made sufficiently large. For this reason, when the container 10 floating on the medium 51 is tilted, the action of the widening portion 40 of moving the center of buoyancy of the container 10 by a large amount and stably exerting a restoring force on the container 10 is increased. It can be done quickly.

Further, the expanding portion 40 may include a bag-like portion 44 containing the contents B that expand when heated. FIG. 19 is a perspective view showing a container 10 in a modified example in which the expanding portion 40 has a bag-like portion 44. FIG. 20 is a side view showing a state in which the container 10 is floated on the medium 51 and the bag-shaped portion 44 of the container 10 in FIG. 19 is warmed. In the example shown in FIGS. 19 and 20, the expanding portion 40 has two bag-like portions 44 that accommodate the contents B inside the bag interior 44a. The content B accommodated in the bag-shaped portion 44 is, for example, air.

Since the expanding portion 40 has the bag-like portion 44 containing the contents B, the volume V ₁ of the expanding portion 40 increases when it is heated and decreases when it is cooled. As a result, when storing the container 5 containing the frozen contents _C , as shown in FIG. can do. Therefore, the space required for storing the container 5 containing the contents can be reduced. Furthermore, when the container 5 containing the contents is floated on the medium 51 to thaw the contents C, the contents B are warmed by heat exchange with the medium 51, and as shown in FIG. ₁ increases. For this reason, when thawing the contained object C, the volume V ₁ of the expanding part 40 is made sufficiently large so that the medium 51 is not immersed in the medium 51 so that the planned extraction opening part 16 of the container body 20 of the container 10 is not submerged in the medium 51. The effect of the expanding portion 40 to further stabilize the floating state can be further improved.

When the container 10 is floated on the medium 51, the contents B are warmed and expanded, so that the bag-like portion 44 stands up toward the side away from the container body 20, as shown in FIG. It may be. As a result, when the container 5 containing the stored material is floated on the medium 51 and the stored material C is thawed, the expanding portion 40 and the accommodating portion 21 of the container body 20 are separated from each other. For this reason, the storage part 21 and the medium 51 can be brought into contact with each other, and heat exchange between the medium 51 and the contents C stored in the storage part 21 can be efficiently caused.

When the content B accommodated in the bag-shaped part 44 has a volume V ₁ of the spreading part and the temperature of the content B is the first temperature, the following equation (1) does not hold; however, when the temperature of the content B is When the second temperature is higher than the first temperature, the expansion may be performed so that equation (1) is satisfied. Further, when the volume V ₁ of the expanding portion 40 is the temperature of the content B is the first temperature, X (%) in the following equation (2) is less than 5%, but the temperature of the content B is the second temperature. When the temperature is high, the expansion may be performed so that X (%) in formula (2) becomes 5% or more. Further, when the temperature of the content B is the first temperature, the value obtained by dividing the volume V ₁ of the expanding portion 40 by the area of the connecting surface 29a does not exceed 5 (mm ³ /mm ² ), but the content B When the temperature is the second temperature, the expanded portion 40 may be expanded such that the volume V ₁ of the expanded portion 40 divided by the area of the connecting surface 29a becomes 5 (mm ³ /mm ² ) or more. The first temperature is, for example, the temperature of the contents B when storing the container 5 containing the contents C in a frozen state, and is, for example, −196° C. or higher and −20° C. or lower. The second temperature is, for example, the temperature of the content B when the container 5 containing the content is floated on the medium 51 and the content C is thawed. For example, when the stored material C in a frozen state is a frozen cell, the second temperature is a temperature suitable for culturing the cells, for example, 20°C or more and 40°C or less, preferably 37°C.

Further, the container 10 may further include a string portion 60 having one end connected to a portion of the widening portion 40 and the other end connected to a portion of the container body 20. FIG. 21 is a perspective view showing a container 10 in a modified example including a string portion 60. In the example shown in FIG. 21, one end of the string portion 60 is connected to an end portion 40c of the spread portion 40 on the opposite side to the side connected to the connecting portion 29. Further, in the example shown in FIG. 21, the other end of the string portion 60 is connected to the first end portion 241 of the container body 20. Although not shown, the other end of the string portion 60 may be connected to a side surface of the container body 20 closer to the first end portion 241 than the connecting portion 29 in the first direction d1. The string part 60 prevents the movement of the spread part 40 so that when the container 10 is floated on the medium 51, the end 40c of the spread part 40 does not move beyond a certain level toward the second end 242 in the first direction d1. limit. When the container 10 is floated on the medium 51, the end portion 40c of the expanding portion 40 can be prevented from moving away from the medium 51. Therefore, the volume of the portion of the widened portion 40 that sinks below the interface 51a can be made larger. As a result, the volume of the portion of the container body 20 that sinks below the interface 51a can be kept small, and the adhesion of the medium 51 to the planned extraction opening portion 16 can be suppressed.

Further, in the above-described embodiment and each modified example, an example in which the connecting portion 29 extends linearly in the second direction d2 has been described, but the form of the connecting portion 29 is not limited to this. As an example, the connecting portion 29 has a curved shape as shown in FIG. 22 . In the example shown in FIG. 22, the connecting portion 29 has an arch-like shape that is convex toward the second end portion 242 side. Further, as shown in FIG. 23, the connecting portion 29 may be provided in a part of the portion where the widening portion 40 is located in the second direction d2. In the example shown in FIG. 23, the widening portion 40 and the container body 20 are fixed by eyelets 25. In the example shown in FIG. In the example shown in FIG. 23, the container body 20 has a through hole 22e in the first joint portion 22b, and the expanding portion 40 and the container body 20 are fixed by an eyelet inserted into the through hole 22e. In this case, the annular portion of the container body 20 that is fixed to the widening portion 40 by the eyelet 25 corresponds to the connecting portion 29 .

It is thought that the expanded portion 40 is likely to break at the boundary between the portion connected to the connecting portion 29 and the portion not connected to the connecting portion 29. Here, as shown in FIG. 22, the connecting portion 29 has a curved shape, or as shown in FIG. 23, the connecting portion 29 is provided in a part of the portion where the widening portion 40 is located in the second direction d2. By doing so, the following effects can be obtained. The boundary between the part of the widening part 40 connected to the connecting part 29 and the part not connected to the connecting part 29 is no longer formed so as to cross the widening part 40 in a straight line. For this reason, the widening portion 40 is larger than when the boundary between the portion of the widening portion 40 connected to the connecting portion 29 and the portion not connected to the connecting portion 29 crosses the widening portion 40 in a straight line. Less likely to break in a straight line. Thereby, when the container 10 is floated on the medium 51, the angle at which the spread part 40 bends can be suppressed to a small value, and the end 40c of the spread part 40 can be prevented from moving largely toward the second end 242 in the direction d1. Therefore, the volume of the portion of the widened portion 40 that sinks below the interface 51a can be made larger. As a result, the volume of the portion of the container body 20 that sinks below the interface 51a can be kept small, and the adhesion of the medium 51 to the planned extraction opening portion 16 can be suppressed.

Furthermore, in the specific example described above, the case where the surface of the film material 23 is flat has been described. However, the shape of the film material 23 is not limited to this. FIG. 24 is a partial sectional view showing a part of a cross section of a modified example of the film material 23 taken along a plane perpendicular to the first direction d1. Further, FIG. 25 is a partial sectional view showing a part of a cross section of another modified example of the film material 23 taken along a plane perpendicular to the first direction d1. As shown in FIG. 24, the film material 23 may have a shape in which an inner surface 23a located on the inside of the container 10 and an outer surface 23b located on the outside of the container 10 have a plurality of grooves 23c. The groove portion 23c extends, for example, in the first direction d1. This makes it possible to sufficiently increase the contact area between the stored object C and the film material 23 and the contact area between the film material 23 and the medium 51. Thereby, the efficiency of heat exchange between the stored object C and the medium 51 can be increased.

Moreover, as shown in FIG. 25, either one of the inner surface 23a and the outer surface 23b of the film material 23 may have a shape having a plurality of unevenness in a cross section perpendicular to the first direction d1. In the example shown in FIG. 25, the inner surface 23a has a shape having a plurality of grooves 23c, and the outer surface 23b is a flat surface. Thereby, the contact area between the stored object C and the film material 23 can be made sufficiently large. Therefore, especially when the specific heat of the film material 23 is smaller than the specific heat of the contained object C, by increasing the efficiency of heat exchange between the contained object C and the film material 23, the heat exchange between the contained object C and the medium 51 is improved. efficiency can be increased.

Further, the accommodating portion 21 may include an accommodating portion detail 21b having a smaller dimension in the direction perpendicular to the first direction d1 than a portion of the container body 20 closer to the second end portion 242 than the connecting portion 29. good. FIG. 26 is a perspective view showing a state before the opening 28 on the second end 242 side of the container 10 according to a modified example in which the accommodating part 21 has the accommodating part detail 21b is sealed. In the example shown in FIG. 26, the container body 20 has a starting portion 26 and an opening 28. In the example shown in FIG. FIG. 27 is a perspective view showing a state before the opening 28 on the second end 242 side of the container 10 in another modified example in which the accommodating part 21 has the accommodating part detail 21b is sealed. In the example shown in FIG. 27, the container 10 includes a cylindrical member 30, and the outer end 31 of the cylindrical member 30 is open.

As shown in FIGS. 26 and 27, the dimension t5 of the accommodating part detail 21b in the second direction d2 perpendicular to the first direction d1 is the dimension of the part of the container body 20 closer to the second end 242 than the connecting part 29. (for example, dimension t6). Note that if the dimensions of the portion of the container body 20 closer to the second end 242 than the connection portion 29 are not constant, the dimension t5 is the same as the dimension t5 of the portion of the container body 20 closer to the second end 242 than the connection portion 29. Less than the maximum value of the dimension. It is preferable that the volume of the accommodating part detail 21b is 70% or more of the entire volume of the accommodating part 21.

Since the dimension t5 of the storage part detail 21b is smaller than the dimension t6, when the container 10 is floated on the medium 51, it is located at the center of the interior of the storage part detail 21b in the direction perpendicular to the first direction d1. The distance between the stored object C and the medium 51 can be kept small. Thereby, it is possible to efficiently exchange heat between the medium 51 and the stored object C accommodated in the accommodating part detail 21b. Also, since the dimension t6 of the portion of the container body 20 closer to the second end 242 than the connection portion 29 is larger than the dimension t5, the portion of the container body 20 closer to the second end 242 than the connection portion 29 can be removed. In forming the opening, a larger extraction opening can be formed. This makes it easier to take out the contents C from inside the container 10 through the take-out opening. In particular, the operation of inserting the tip of a pipette or the like into the container 10 through the take-out opening and dispensing the contents C using the pipette or the like can be made easier.

Further, as shown in FIG. 28, a label 70 may be attached to the widened portion 40. The label 70 is attached to the flare 40 by, for example, being adhered to the flare 40 using an adhesive or by heat welding. On one side of the label 70, information such as the contents of the contents C accommodated in the container 10 and how to use the container 10 is written, for example. By attaching the label 70 to the expanding portion 40, when the container 10 is floated on the medium 51 as shown in FIG. 29, the side of the label 70 on which information is written faces upward. Therefore, the user of the container 10 can visually recognize the information on the label 70 while the container 10 is floating on the medium 51. Furthermore, by attaching the label 70 to the spread portion 40, the components of the adhesive and the like enter the inside of the container 10 when an adhesive is used to attach the label 70, compared to when attaching the label 70 to the film material 23. Putting things away is more restrained.

Further, although not illustrated, the container 10 may further include a mark portion located closer to the second end portion 242 than the connecting portion 29 in the first direction d1. The mark portion is, for example, a protruding portion provided on the surface of the film material 23 on the outside of the container 10. The mark portion may be a groove-shaped portion provided on the surface of the film material 23 on the outside of the container 10. The mark indicates, for example, that the extraction opening should be formed closer to the second end 242 than the mark in the first direction d1.

As yet another modification example, in the container 10 described above, the accommodating portion 21 of the container body 20 is maintained in a sterile state, and the contained object C containing biological material is directly accommodated in the accommodating portion of the container body 20. However, it is not limited to this example. A contained object C containing a biological substance is stored in an inner bag separate from the container 10, the contained object C is frozen and stored within this inner bag, and the inner bag containing the contained object C is stored in the above-mentioned container 10. The decompression operation may be performed using the medium 51. In this example, the container 10 may be used only during thawing operations. Even in such an example of use, the attachment of the medium 51 to the take-out opening of the container 10 is effectively prevented. can be avoided.

Furthermore, in the above-described example, an example was shown in which the contents of the container 10 contained a biological material, but the present invention is not limited to this example. Even for various containers that do not contain biological substances, it may be desired to avoid contamination with a medium used for heat exchange, adhesion of the medium, contact with the medium, etc. For example, when food is subjected to heat exchange with heated water or cooled water, adhesion of water to the food may be desired. The container 10 having the widening portion 40 according to the embodiment described above can be applied to various types of objects without being limited to objects containing biological materials, and can be applied to various objects during heat exchange of a medium. It is possible to effectively prevent the medium from coming into contact with, adhering to, or mixing with the contents.

That is, the container 10 is a container used for heat exchange between the contents C and the medium 51, and is connected to a container body 20 having a housing part 21 for storing the contents C, and an outer surface of the container body 20. A widening portion 40 that is a floating member 401 having a specific gravity smaller than that of the medium 51 may be included. Further, the container 10 includes a container body 20 having a housing part 21 for storing the contents C, and a widening part 40 connected to the outer surface of the container body 20. Even if it is connected to the container body 20 at a position between the stored object C and a take-out opening that communicates the storage section C to the outside when taking out the storage object C from the storage section 21. good. According to these containers 10, it is possible to solve the problem of effectively preventing the medium 51 for thawing the contents C from adhering to the contents C of the container 10.

Next, the present disclosure will be explained in more detail with reference to examples, but the present disclosure is not limited to the description of the following examples unless it exceeds the gist thereof.

(Example 1)
As a first embodiment, a container 5 containing a container 5 including a container 10 having the form shown in FIGS. 1 to 4 and a frozen container C stored inside the container 10 is used. floated above. Then, the container 5 containing the contents was floated on the medium 51 in a state where the second end 242 of the container body 20 was not immersed in the medium 51, and it was evaluated whether the container 5 containing the contents floated stably.

The material of the container 10 was polyethylene. Moreover, the dimension t7 of the container body 20 in the first direction d1 shown in FIG. 2 was 12 cm. Further, the dimension t4 of the accommodating portion 21 in the second direction d2 was 9.5 cm. Further, when the container 10 was in the state shown in FIG. 2, the dimension t1 of the widening portion 40 in the first direction d1 was 1 cm. Further, the dimension t2 of the widened portion 40 in the second direction d2 was 8 cm. Further, the dimension t8 of the spread connection portion 41 in the first direction d1 was 0.5 cm. Further, the distance t9 between the first joint portion 22b and the spread connection portion 41 in the first direction d1 was 0.5 cm. Further, the width t10 of the joint portion 22 was 0.5 cm. Further, the thickness of the film material 23 was 0.026 cm.

Regarding the container 10, the volume _V2 shown in FIGS. 7 and 18 is the volume of the film material 23 located closer to the first end 241 than the second end 29c of the connecting portion 29 in the first direction d1. , the volume of the container 10, and the total volume was calculated. The volume of the container 10 is also referred to as volume _V4 . The volume _V4 was calculated based on the formula described in "Film for Food Packaging (published by Nippo Publishing)" written by Hiroshi Osuka, that is, the following formula (6). In equation (6), a is the distance between the joint 22 located on the third side 20c and the joint 22 located on the fourth side 20d in the second direction d2. In addition, S in formula (6) is located closer to the first end 241 than the second end 29c when the container 10 is observed from a direction perpendicular to the surface of the film material 23, and is connected This is the area of the portion where the portion 22 is not formed. In the case of the container 10 shown in FIG. 2, S is the product of the above-mentioned a and the distance t11 between the second end side end 29c and the joint 22 located on the first side 20a in the first direction d1. expressed.

The value obtained by dividing the volume V ₁ of the widening portion 40 by the area of the connecting surface 29a was 0.10 (mm ³ /mm ² ). Further, the ratio X (%) expressed by the above formula (2) was 0.22%. Moreover, since the container 10 of Example 1 did not have the spreading assisting part 46, the ratio Y (%) expressed by the above formula (3) was 0%.

The medium 51 in which the container 10 floated was water at 37°C. When the density ρ of the medium 51, which is the liquid 511, is 1 g/cm ³ , the value of ρ(V ₁ +V ₂ )−W ₁ , which is the left side of the above equation (4), is 180.56 (g). Ta.

Containment C was frozen cells obtained by freezing a cell suspension. The mass of the object C housed inside the container 10 was 15.00 (g).

(Example 2)
As Example 2, except for the points described below, the container 5 containing the contents was stably placed on the medium 51 by the same method as in Example 1 for the container 5 containing the contents similar to the container 5 containing the contents in Example 1. We evaluated whether it floated. In Example 2, the dimension t1 was 1.5 cm. Further, the value obtained by dividing the volume V ₁ by the area of the connection surface 29a was 0.16 (mm ³ /mm ² ). Moreover, the ratio X (%) expressed by the above formula (2) was 0.33%. Further, the value of ρ(V ₁ +V ₂ )−W ₁ which is the left side of the above equation (4) was 180.57 (g).

(Example 3)
As Example 3, except for the points described below, the container 5 containing the contents was stably placed on the medium 51 by the same method as in Example 1 for the container 5 containing the contents similar to the container 5 containing the contents in Example 1. We evaluated whether it floated. In Example 3, the dimension t1 was 3 cm. Further, the value obtained by dividing the volume V ₁ by the area of the connection surface 29a was 0.31 (mm ³ /mm ² ). Further, the ratio X (%) expressed by the above formula (2) was 0.66%. Further, the value of ρ(V ₁ +V ₂ )−W ₁ which is the left side of the above equation (4) was 180.59 (g).

(Example 4)
As Example 4, except for the points described below, the container 5 containing the contents was stably placed on the medium 51 by the same method as in Example 1 for the container 5 containing the contents similar to the container 5 containing the contents in Example 1. We evaluated whether it floated. In Example 4, the dimension t1 was 6 cm. Further, the value obtained by dividing the volume V ₁ by the area of the connection surface 29a was 0.62 (mm ³ /mm ² ). Further, the ratio X (%) expressed by the above formula (2) was 1.31%. Further, the value of ρ(V ₁ +V ₂ )−W ₁ which is the left side of the above equation (4) was 180.64 (g).

(Example 5)
As Example 5, except for the points described below, the container 5 containing the contents was stably placed on the medium 51 by the same method as in Example 1 for the container 5 containing the contents similar to the container 5 containing the contents in Example 1. We evaluated whether it floated. In Example 5, the dimension t1 was 8 cm. Further, the value obtained by dividing the volume V ₁ by the area of the connection surface 29a was 0.83 (mm ³ /mm ² ). Moreover, the ratio X (%) expressed by the above formula (2) was 1.75%. Further, the value of ρ(V ₁ +V ₂ )−W ₁ which is the left side of the above equation (4) was 180.67 (g).

(Example 6)
As Example 6, except for the points described below, the container 5 containing the contents was stably placed on the medium 51 by the same method as in Example 1 for the container 5 containing the contents similar to the container 5 containing the contents in Example 1. We evaluated whether it floated. In Example 6, the container 10 shown in FIGS. 1 to 4 was further provided with a spreading assisting portion 46 shown in FIGS. 17 and 18. In Example 6, the dimension t1 was 1.5 cm. Further, the value obtained by dividing the volume V ₁ by the area of the connection surface 29a was 0.25 (mm ³ /mm ² ). Moreover, the ratio X (%) expressed by the above formula (2) was 0.54%. Further, the ratio Y (%) expressed by the above formula (3) was 0.21%. Further, the value of ρ(V ₁ +V ₂ )−W ₁ which is the left side of the above equation (4) was 180.20 (g).

(Evaluation results)
In any of Examples 1 to 6, it was confirmed that the container 5 containing the contents was stably floating on the medium 51 with the second end 242 of the container body 20 not immersed in the medium 51. Therefore, according to the container 10 of the present disclosure, it has been found that adhesion of the medium 51 to the scheduled extraction opening portion 16 located on the second end 242 side of the container body 20 is suppressed.

In order to evaluate the stability of the contents-containing containers 5 of Examples 1 to 5 when floating on the rippling medium 51, the following evaluation was further performed. First, a water bath (manufactured by As One Corporation, trade name "Thermal Robo TR-2AR") having a water tank filled with water as the medium 51 and a jet pump (discharge rate: 10 L/min) was prepared. Next, the jet pump was activated to cause the water in the tank to ripple. Next, the container 5 containing the contents was floated on the water surface approximately 25 cm away from the jet pump so that the second end 242 was not immersed in water, and it was evaluated whether the container 5 containing the contents floated stably. The temperature of the water was 37°C.

As a result of evaluation on the undulating medium 51, the value obtained by dividing the volume V ₁ by the area of the connecting surface 29a is less than 0.15 (mm ³ /mm ² ), and the ratio X (%) is less than 0.3%. The object-containing container 5 of Example 1 did not float stably, and the second end 242 came into contact with water. On the other hand, Examples 2 to 5 in which the value obtained by dividing the volume V ₁ by the area of the connecting surface 29a is 0.15 (mm ³ /mm ² ) or more, and the ratio X (%) is 0.3% or more. The container 5 containing the contents floated stably. Therefore, according to the container 10 of the present disclosure, the value obtained by dividing the volume V ₁ by the area of the connecting surface 29a is set to 0.15 (mm ³ /mm ² ), or the ratio X (%) is set to 0.3 % or more, it has been found that the container 5 containing the contents can be particularly stably floated on the medium 51 in a state where the second end 242 of the container body 20 is not immersed in the medium 51.

Regarding the containers 5 containing contents in Examples 2 and 6, the following evaluation was further performed in order to particularly evaluate the stability when floating on the undulating medium 51. First, a water bath (manufactured by As One Corporation, trade name "Thermal Robo TR-2AR") having a water tank filled with water as the medium 51 and a jet pump (discharge rate: 10 L/min) was prepared. Next, the jet pump was activated to cause the water in the tank to ripple. Next, the container 5 containing the contents was floated around the jet pump so that the second end 242 was not immersed in water, and it was evaluated whether the container 5 containing the contents floated stably. The temperature of the water was 37°C.

As a result of evaluation on the rippling medium 51, the container 5 containing the contents of Example 2, which does not have the spreading auxiliary part 46, did not float stably, and the second end 242 touched the water. On the other hand, the object-containing container 5 of Example 6 having the spreading auxiliary part 46 floated stably. Therefore, it has been found that the container 5 containing the contents can be particularly stably floated on the medium 51 with the second end 242 of the container body 20 not immersed in the medium 51 by the spreading assisting portion 46. .

C Contained object 5 Container with stored object 10 Container 16 Expected extraction opening section 20 Container body 21 Container section 21b Container section details 22 Joining section 22a Easy joining section 22b First joining section 22c Second joining section 22d Opening section joining section 23 Film material 241 First end 242 Second end 25 Eyelet 26 Starting part 27 Cutting part 28 Opening part 29 Connection part 29a Connection surface 29b First end side end 30 Cylindrical member 31 External end 40 Spreading part 401 Floating member 40A 1 spreading part 40B 2nd spreading part 40c End part 41 Spreading connection part 42 Extension part 43 Spreading main body part 44 Bag-like part 45 Film 46 Spreading auxiliary part 50 Medium holding container 51 Medium 511 Liquid 51a Interface 60 String part 70 Label

Claims (30)

A container containing biological material,
A film material having a first face and a second face facing each other, the film material having at least a part of the part where the first face and the second face face each other are joined, and the film material has a first end and a second face. a container body having a second end opposite the first end in a first direction;
a widening part connected to a connecting part located between the first end part and the second end part in the first direction of the container main body,
The flared portion has a first flared portion and a second flared portion, the first flared portion is attached to one of the first surface and the second surface, and the second flared portion is attached to the first flared portion. attached to the other of the surface and the second surface,
The container main body has a storage part located closer to the first end than the first end of the connection part in the first direction, and stores the contents therein,
The second end is closed by a joint formed by joining the film material,
The container body includes a notch on the second end side of the connecting portion in the first direction, and has a starting portion that is a cutting start position of the container body, or
The container, wherein a first joint part of the joint part located closer to the second end than the connecting part in the first direction has a sealing strength of 3 N/15 mm or more and 6 N/15 mm or less at least partially. A container containing biological material,
A film material having a first surface and a second surface that face each other, the film material having at least a part of the portion where the first surface and the second surface face each other are joined, and the film material has a first end portion and a second surface that faces each other. a container body having a second end opposite the first end in a first direction;
a widening portion connected to a connecting portion located between the first end and the second end of the container body in the first direction;
The expanded portion has a first expanded portion and a second expanded portion, the first expanded portion is attached to one of the first surface and the second surface, and the second expanded portion is attached to the first expanded portion. attached to the other of the surface and the second surface,
The container main body has a storage part that is located closer to the first end than the first end of the connection part in the first direction and stores the contents therein,
further comprising a cylindrical member connected to the second end side of the container body so as to communicate with the accommodating part,
A container, wherein an outer end of the cylindrical member located outside the container body is closed. A container containing biological material,
A film material having a first face and a second face facing each other, the film material having at least a part of the part where the first face and the second face face each other are joined, and the film material has a first end and a second face. a container body having a second end opposite the first end in a first direction;
a widening part connected to a connecting part located between the first end part and the second end part in the first direction of the container main body,
The flared portion has a first flared portion and a second flared portion, the first flared portion is attached to one of the first surface and the second surface, and the second flared portion is attached to the first flared portion. attached to the other of the surface and the second surface,
The container main body has a storage part located closer to the first end than the first end of the connection part in the first direction, and stores the contents therein,
The container main body further includes a first fitting part and a second fitting part which are provided on the inner surface of the film material closer to the second end than the connecting part in the first direction and are capable of fitting into each other. have,
The second end of the container is closed by fitting the first fitting part and the second fitting part. A container containing biological material,
A film material having a first face and a second face facing each other, the film material having at least a part of the part where the first face and the second face face each other are joined, and the film material has a first end and a second face. a container body having a second end opposite the first end in a first direction;
a widening part connected to a connecting part located between the first end part and the second end part in the first direction of the container main body,
The flared portion has a first flared portion and a second flared portion, the first flared portion is attached to one of the first surface and the second surface, and the second flared portion is attached to the first flared portion. attached to the other of the surface and the second surface,
The container main body has a storage part located closer to the first end than the first end of the connection part in the first direction, and stores the contents therein,
The accommodating portion is a container that communicates with the outside of the container main body via a portion of the container main body that is located closer to the second end than the connecting portion in the first direction. further comprising a cylindrical member connected to the second end side of the container body so as to communicate with the accommodating part,
The container according to claim 4, wherein among the ends of the cylindrical member, an external end located outside the container main body is open. The container body has an opening formed between the facing film materials closer to the second end than the connection portion in the first direction and allowing the accommodating portion to communicate with the outside of the container body. death,
5. The container according to claim 4, wherein the container body includes a notch closer to the second end than the connecting portion in the first direction, and has a starting portion that is a cutting start position of the container body. The container body has an opening formed between the facing film materials closer to the second end than the connection portion in the first direction and allowing the accommodating portion to communicate with the outside of the container body. death,
According to claim 4, when the film material is joined at the opening, the opening joint formed by joining the film material at the opening has a seal strength of 3 N/15 mm or more and 6 N/15 mm or less. container. The container main body further includes a first fitting part and a second fitting part which are provided on the inner surface of the film material closer to the second end than the connecting part in the first direction and are capable of fitting into each other. have,
The container according to claim 4, wherein the accommodating portion communicates with the outside of the container main body via between the first fitting portion and the second fitting portion. 1 . The accommodating portion has a accommodating portion detail having a smaller dimension in a second direction perpendicular to the first direction than a portion of the container body closer to the second end than the connecting portion. 9. The container according to any one of 8. The container according to any one of claims 1 to 9, wherein the size of the accommodating part in the first direction is larger than the size of the accommodating part in a second direction perpendicular to the first direction. The container according to any one of claims 1 to 10, wherein the thickness of the film material in the accommodating portion of the container body is 1 mm or less. The container according to any one of claims 1 to 11, wherein the film material in the accommodating portion of the container main body has a thermal conductivity of 0.1 W/m·K or more. 13. The widening portion according to claim 1, wherein when the container is immersed in liquid from the first end side of the container body, the widening portion projects away from a portion of the container body other than the connecting portion. A container according to any one of the items. The volume of the expanding portion is V ₁ , the total volume of the volume of the container body including the inside of the portion where the connecting portion is located in the first direction and the volume of the accommodating portion is V ₂ , and the container The container according to any one of claims 1 to 13, wherein the following formula (1) holds when the mass of is W ₁ and the density of water is ρ _W.
The container according to claim 14, wherein the ratio X (%) expressed by the following formula (2) is 0.3% or more.
Claims 1 to 15, wherein a value obtained by dividing the volume of the widening portion by the area of a connecting surface forming a boundary between the widening portion and the connecting portion is 0.15 (mm ³ /mm ² ) or more. A container according to any one of the items. The widening portion includes a widening main body portion that spreads in a direction crossing the first direction when the container is immersed in liquid from the first end side of the container main body, and a widening main body portion that spreads from the container main body of the widening main body portion. 17. A container according to any one of claims 1 to 16, comprising a spreading aid connected to the spaced apart ends. The volume of the expanding portion is V ₁ , and the total volume of the volume of the container body including the inside of the portion where the connecting portion is located in the first direction and the volume of the accommodating portion including the inside is V 1 . _2. The container according to claim 17, wherein the ratio Y (%) expressed by the following formula (3) is 0.2% or more, where the volume of the expansion auxiliary part is _V3 .
The container according to any one of claims 1 to 18, wherein the widening portion has symmetry when viewed from the first direction. The container according to any one of claims 1 to 19, wherein the expanding portion has a bag-like portion containing a content that expands when heated. 21. The container according to claim 20, wherein the bag-shaped portion stands up toward a side away from the container main body when the contents are heated and expanded. 3. The container of claim 2, further comprising a lid removably closing the outer end. 3. The container of claim 2, wherein the cylindrical member has a thin wall portion closing the outer end. 3. The container according to claim 2, wherein the outer end is closed by a seal formed by joining opposing sides of the cylindrical member. A container containing a container comprising the container according to any one of claims 1 to 3 and a frozen container located inside the container,
A container containing a stored material, wherein 50% by mass or more of the frozen stored material located inside the container is stored in the storage section. A supplying step of supplying the contents to the storage part via the cylindrical member of the container according to claim 5,
A method for manufacturing a container containing a contained object, comprising a sealing step of sealing the outer end. A supplying step of supplying the contents to the storage section through the opening of the container according to claim 6 or 7;
A method for manufacturing a container containing a contained object, comprising a sealing step of sealing the opening. A thawing method for thawing a frozen content stored in the storage part of the container according to any one of claims 1 to 3 by heat exchange with a liquid,
A thawing method comprising a thawing step of immersing the container in the liquid from the first end side of the container body. The volume of the expanding portion is V ₁ , and the total volume of the volume of the container body including the inside of the portion where the connecting portion is located in the first direction and the volume of the accommodating portion including the inside is V 1 . _2. The thawing method according to claim 28, wherein the following formula (1) holds true when the mass of the container is W ₁ and the density of the liquid is ρ.
A method for producing a cell preparation, comprising: thawing frozen cells accommodated in the storage part of the container according to any one of claims 1 to 3 by heat exchange with a liquid. ,
A method for producing a cell preparation, comprising a thawing step of immersing the container in the liquid from the first end side of the container main body.