KR200353001Y1 - Preventing Device of Releasing a Case from a Rack for Bessel Holder of Cryo Preservation - Google Patents

Abstract

The present invention relates to a case detachment prevention device in a rack for cryogenic cryopreservation vessel holder, and in particular, several cases comprising a body for receiving a predetermined volume and a cover covering the opening of the body; A rack having a frame formed along an edge to open one side as an elongated hexahedron, a partition partitioning the case to a height at which the case can be slid, and a rack attached to an upper surface of the elongated hexahedron; In the holder for cryogenic cryopreservation vessel consisting of a fixing means for preventing the cases stored in the space portion of the rack partitioned by the partition from being separated from the rack, the fixing means is respectively placed on the case entrance of the rack or the opposite side of the entrance It is formed so that the cases can be fixed and released in units.

Therefore, it is possible to control the withdrawal of each of the several cases that are detachably installed in units in the rack through a separate fixing means, which can be prevented from being easily detached from the rack during transport and handling, as well as withdraw the desired case Other cases can completely prevent contamination between samples, difficulty in handling, and breakage during transportation and handling due to detachment from the rack.

Description

Preserving Device of Releasing a Case from a Rack for Bessel Holder of Cryo Preservation}

The present invention relates to a case detachment prevention device in a rack for a cryogenic cryopreservation vessel holder, and more particularly, to be detachable to a rack, which is a holder for a biological sample used in a vessel for cryogenic preservation of a biological sample. It is designed to prevent contamination between samples, difficulty in handling, damage during transportation and handling by preventing cases easily installed from the rack during transportation and handling.

In general, biological materials such as blood, plasma, lymph, semen, vaginal fluid, tissues or organs or fragments thereof, microbial cells, DNA or RNA, vectors, hybridomas, bone marrow cells, and cancer cells are the external environment due to the nature of life. If left untreated, it changes over time and deteriorates.

The most effective way to stop the degradation process in such biological materials is known to freeze the biological materials and store them at cryogenic temperatures. Such cryopreservation is currently widely used in the biological and biopharmaceutical fields.

Cooling and freezing of biological materials is a very complex process involving chemical and physiological changes, and the effects of these changes on biological materials are known to depend on the structural complexity of the biological materials.

Although biological materials can be stored by some other means besides cryopreservation, cryogenic preservation is the only preservation method that can minimize changes in biological materials.

Cryopreservation is a preservation technology for biological materials that was proposed over 50 years ago and has been in use for decades. The most effective and easy way to bring the preservation system into a cold environment is to deposit it in liquid nitrogen to ensure the stability of the biological material. I can make it.

The most important factor in this cryopreservation method is to maintain the cryopreservation system in a constant temperature range below the minimum critical threshold temperature of the biological material, thereby impairing or gradual changes in the properties of the biological material during storage. Happening or restorative action may be prevented.

For example, it is known that the critical temperature of living cells is -130 ° C or lower, and therefore it is desirable to maintain and preserve the cells in a liquid nitrogen freezer at -150 ° C to -196 ° C.

In the cryopreservation method, it is generally a matter of safety, sample integrity and cross contamination.

However, if the sample is directly stored in liquid nitrogen, if the sample container (vinyl pack or plastic container, etc.) is incompletely sealed, liquid nitrogen will penetrate into the sample and burst upon opening the pack or container, resulting in partial loss or contamination of the sample. There is the potential to cause.

Therefore, a carrier containing a large number of samples or samples is usually stored frozen in a carrier contained in liquid nitrogen. The carrier is heavy to lift and the liquid nitrogen must be discharged from the carrier before taking out the sample. Can often lead to splashing of liquid nitrogen, resulting in potential freezing burns.

In addition, cases have been reported in which pathogen transfer occurs between samples stored in liquid nitrogen.

Thus, proper maintenance and handling of biological materials in cold storage is imperative for maintaining the stability of biological materials, for which low-temperature materials such as liquid nitrogen are contained in the chambers of liquid nitrogen tanks. Soaking in the liquid nitrogen; It consists of a carrier having a constant content and only an upper portion open, a carrier extending long from the upper end of the conventional body, and a carrier made of a lead derived from the bar.

In addition, the carrier distinguishes which samples are stored in the carrier by attaching a tag having a color to the lid by storing the lid on the lid of the tank. Many kinds of samples are mixed and stored in the carrier, so it is inconvenient for use / storage.When taking out the carrier from the tank, hold the lid and lift it up a little, so that the normal body comes out of the liquid nitrogen for a predetermined time. The liquid nitrogen flows down completely, and then lifts it out of the neck of the tank through the long bar to the ordinary body, in which case the handle is not easy to handle because the operator only needs to lift the lid. As such, liquid nitrogen (-196 ° C) splashes on the operator, causing unintended injury (cooling burns, etc.). It has been commonplace there is a problem.

Therefore, Applicant is to be stored in a chamber of a double nitrogen tank (Liquid nitrogen tank) in which the cryogenic material such as liquid nitrogen is contained therein to solve the above problems; Developed and registered in Korea as a "sample holder for cryogenic cryopreservation vessel of biological material" consisting of a case having a unit standard, a rack containing a plurality of the case together with the case, and a clasp that prevents the case from being freed from the rack. Registered as Utility Model Publication No. 20-0303608.

However, the sample holder for the cryogenic cryopreservation vessel of the biological material is a double nitrogen tank (Liquid nitrogen tank) in which the cryogenic material such as liquid nitrogen 41 is contained therein, as shown in FIGS. 9 and 10. Stored in the chamber 4 of the chamber; A body 11 having a hinge hole 111 having a long hole at a side end, and a locking jaw 112 at an upper end thereof, and accommodating predetermined contents; And a head 121 formed at both sides to guide the side of the body 11 to the inside, a hole 122 bored at the center, and a hinge groove 111 of the body 11. A cover 12 having a hinge 123 and covering the body 11; A case (1) which becomes;

Frame 21 is formed along the corner to open one side as a long cube; A partition 22 partitioning the elongated hexahedron to a height at which the case 1 can be slid; And a handle (23) attached to an upper surface of the elongated hexahedron.

In the partition 22 of the rack (2) has a configuration consisting of a latch groove 221 which is respectively drilled in the entrance side and the clasp 24 through the respective latch grooves (221, 221, ...) at the same time.

Therefore, a large amount of samples such as blood bone marrow can be easily stored and stored properly, and by specifying the samples to be stored on a per unit basis, contamination between the samples and difficulty in handling can be prevented at the source. It was possible to prevent contamination.

However, such a configuration has a form in which a long shackle bent in a cane shape is detachably installed in the partition wall of the rack as a means for preventing the case from being separated from the rack during transportation. In this case, the case of each unit is prevented from being separated from the rack by the latch, but when carrying out any one of several unit cases installed in the rack after transportation, the latch is removed from the rack and the desired case is removed from the rack. There is a problem that cannot be withdrawn.

In other words, if the latch is removed from the rack, all of the case-by-unit cases are released, so if the rack is tilted to one side due to carelessness of the worker in the process of withdrawing the desired case from the rack, only the desired case is not withdrawn. They are also separated from the rack, there is a problem that the sample in the case is damaged, thereby causing contamination and the like.

The present invention has been devised to solve such problems in the related art, and transports and handles a plurality of cases detachably installed in units of a rack, which are holders for biological sample storage, by means of separate fixing means. Not only can it be easily removed from the rack but also the ultra-low temperature can completely prevent contamination or handling difficulties between the samples due to the removal of the desired case from other racks and the difficulty of handling and damage. It is an object of the present invention to provide a case detachment prevention device in a rack for a cryopreservation vessel holder.

The object of the present invention is a number of cases comprising a cover for covering the opening of the body and the body for receiving a predetermined content; A rack having a frame formed along an edge to open one side as an elongated hexahedron, a partition partitioning the case to a height at which the case can be slid, and a rack attached to an upper surface of the elongated hexahedron; In the holder for cryogenic cryopreservation vessel consisting of a fixing means for preventing the cases stored in the space portion of the rack partitioned by the partition from being separated from the rack, the fixing means is respectively placed on the case entrance of the rack or the opposite side of the entrance It can be achieved by forming so that the cases can be fixed and unlocked unit by unit.

Therefore, it is possible to control the withdrawal of each of the several cases that are detachably installed in units in the rack through a separate fixing means, which can be prevented from being easily detached from the rack during transport and handling, as well as withdraw the desired case Other cases can completely prevent contamination between samples, difficulty in handling, and breakage during transportation and handling due to detachment from the rack.

1 is an exploded perspective view of a rack and a case in which an embodiment of the present invention is implemented.

Figure 2 is a side cross-sectional view of some cut according to an embodiment of the present invention device.

Figure 3a, 3b is a plan sectional view of the hinge portion and the fixing portion of the case departure prevention cover in one embodiment of the present invention.

Figure 4 is a partial cutaway front cross-sectional view according to another embodiment of the present invention device.

5 is a partially enlarged cross-sectional view of FIG. 4.

Figure 6 is an exploded perspective view showing another embodiment of the present invention device.

7 is an enlarged cross-sectional view of the main portion of FIG. 6;

Figure 8 is an exemplary use state installed in the chamber with a rack provided with the subject innovation device.

9 is an exploded perspective view of a holder for a conventional cryogenic cryopreservation vessel.

10 is an exploded perspective view of a case for storing a sample.

Explanation of symbols on main parts of drawing

1: case 2: rack

5: fixing means 21: frame

22: partition 23: handle

25: case entrance 51: cover

52: jamming protrusion 53: locking hole

54: leaf spring 55: obstruction

56: hanging groove

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is an exploded perspective view of a rack and a case in which an embodiment of the present invention is implemented, and FIG. 2 is a partial cutaway side cross-sectional view according to an embodiment of the present invention, and FIGS. 3A and 3B illustrate a case of an embodiment of the present invention. The planar cross-sectional view of the hinge portion and the fixing portion of the separation prevention cover is shown.

Figure 4 is a partial cutaway front cross-sectional view according to another embodiment of the present invention device, Figure 5 is a partially enlarged cross-sectional view of Figure 4, Figure 6 is an exploded perspective view showing another embodiment of the present invention device, Figure 7 It is an essential part enlarged cross-sectional view of 6, and FIG. 8 shows the use state example which installed the rack provided with this apparatus in the chamber.

According to this, there are several cases (1) comprising a body (11) for storing a predetermined content and a cover (12) for covering the opening of the body (11); As a long cube, one side has a frame 21 which is open to allow the case 1 to enter and exit along a corner, and has a left and right separation prevention jaw 222 and is partitioned to a height at which the case 1 can be slid and stored. A rack (2) comprising several partitions (22) and a handle (23) attached to an upper surface of the elongated cube; In the holder for cryogenic cryopreservation vessel composed of fixing means (5) to prevent the case (1) stored in the space portion of the rack (2) partitioned by the partition (22) from being separated from the rack (2) ,

The fixing means 5 is installed and formed separately at the case entrance 25 of the rack 2 or the opposite side of the entrance 25, so that the cases 1 can be fixed and released by units. .

1 to 3a and 3b show an embodiment of the fixing means 5 of the present invention, the upper end of the long cover 51 formed to be bent so as to have a cross-section "c" shape of the rack 2 The upper part of the case entrance and exit 25, and the lower end of the cover 51 is formed with a pair of locking projections 52, each of the case entrance 25 of the rack 2, the locking projections 52 are It is characterized in that it is made by punching the engaging hole 53 to be fitted.

4 and 5 show another embodiment of the fixing means 5 according to the present invention, and one or two leaf springs 54 having one or more leaf springs bent into a roughly "∧" shape with good tension. It is characterized by being fixedly installed on the upper or upper and lower surfaces of each of the partitions 22 inlet of the rack 2 (that is, near the case entrance and exit 25).

In addition, Figure 6 and Figure 7 shows another embodiment of the fixing means 5 of the present invention, on both sides of the frame opposite the case entrance and exit 25 of the rack (2) and the front and rear rear side of the case (1) Each of the rectangular locking projections 55 and the locking grooves 56 are formed.

Referring to the operation and effect of the device of the present invention configured as described above are as follows.

First, the main technical configuration of the present invention is a number of cases (1) in the chamber (4) of a double nitrogen tank (Liquid nitrogen tank) in which the cryogenic material such as liquid nitrogen 41 is contained therein as shown in FIG. To the case entrance 25 of the rack 2 or its fastening means 5 such that the cases 1 are not detached from the rack 2 when they are stored and stored in the rack 2 or when they are moved and handled. The case 1 is formed on the frame opposite to the doorway 25 so that the cases 1 can be fixed and released by unit.

At this time, the rack (2) to which the case (1) is individually fixed through the fixing means (5) to install the base (3) on the plate so as to be exposed on the water surface of the liquid nitrogen 41 accommodated in the chamber (4) In this structure, a plurality of piles are placed on the base 3. In this structure, the average temperature in the chamber 4 is about -196 ° C, so that the sample can be appropriately stored.

In the above, the configuration of the case 1 and the rack (2) is the same as the vessel sample holder for the applicant, and detailed description of the technical configuration and their operation relationship is omitted, and the partition 22 will be described below Referring to specific embodiments of the fixing means (5) to prevent the case (1) accommodated in the space portion of the rack (2) partitioned by being separated from the rack (2) as follows.

First, the upper end portion of several long covers 51 formed by bending the fixing means 5 to have a "c" shape in a cross section as in one embodiment is placed on the upper portion of each case entrance 25 of the rack 2. In the case of having a configuration in which a pair of locking projections 52 and a locking hole 53 are drilled at the lower ends of the case entrances and exits 25 of the cover 51 and the rack 2, the rack 2 is normally used. Since the lower end projections 52 of the covers 51 having the upper end moistened at each case entrance and exit 25 of the case are fitted into the engaging holes 53 drilled in the frame 21 of the rack 2, the state is held. The closed state is maintained by the locking action between them.

That is, when the cover 51 respectively formed at the case entrance and exit 25 of the rack 2 is closed by rotating it counterclockwise in the drawing, the pair of engaging protrusions 52 formed at the lower end thereof are the entrance and exit of the rack 2. (25) The case is stored in the space between the partitions 22 of the rack (2) when holding or moving the entire rack (2) is held in the locking hole (53) drilled in the lower part (1) Free detachment from the rack is prevented.

Therefore, the case 1 may be easily prevented from being separated from the rack 2 during transport and handling, thereby completely preventing contamination between samples, difficulty in handling, and damage during transport and handling.

On the other hand, when one of the several cases (1) stored and moved in the rack (2) to remove the rack from the rack means of the fixing means (5), that is, the cover (25) Pulling the lower portion of the 51, the locking protrusion 52 is forced out of the locking hole 53, so that the cover 51 is opened as shown in Figure 1 can be removed only the case through the doorway.

Of course, when the cover is opened and the sample containing the sample is taken out as described above, even if the rack 2 is inclined toward the doorway due to the carelessness of the operator, the other covers remain fixed (that is, closed). It is possible to completely prevent contamination between samples, difficulty in handling, and breakage during transportation and handling due to separation of other cases from the rack.

In addition, one end of one or two leaf springs 54 in which the fixing means 5 is bent into a steel plate having a good tension in a substantially "∧" shape, as in another embodiment, each partition 22 of the rack 2. When the case 1 is fixedly installed on the upper or upper surface of the inlet side, when the case 1 is pushed in through the entrance 25 of the rack 2, the "∧" type leaf spring 54 is in contact with the upper and lower surfaces of the case 1. ) Is deformed like the imaginary line of FIG. 5 by the pressing of the case and the height thereof is lowered, so that the cases 1 may be accommodated in the space formed between the partitions 22 of the rack 2.

Of course, if the case 1 is completely received in this space part and its front part is removed from the upper surface of the spring 54 and the pressing force is lost, the spring 54 is restored to its original state by its restoring force, One lower end or the upper and lower end portions of the lower end or the upper and lower end portions are held in the fixing means 5 that is fixed to the upper or upper and lower surfaces of the respective partitions 22, that is, the “∧” -type leaf spring 54.

Therefore, the case 1 is easily detached from the rack 2 during transportation and handling in a state where several cases 1 are accommodated in the space formed between the partitions 22 of the rack 2 as described above. It can prevent the contamination between samples, difficulty in handling and damage during transportation and handling.

On the other hand, when you want to remove any one of the several cases (1) stored and moved in the rack (2) from the rack when you pull the case 1 strongly toward the doorway 25 when storing the case Similarly, since the spring 54 is deformed, only the desired case can be removed from the storage space of the rack 2.

Of course, at this time, even if the rack 2 is inclined toward the doorway due to the carelessness of the operator when forcibly pulling out only one of the several cases in which the sample is contained, the other cases 1 are connected to the spring 54. It keeps the jammed state to completely prevent the contamination or handling difficulties between the samples due to automatic detachment from the rack when the desired case is taken out, damage during transport and handling.

In addition, the fixing means 5 is engaged with the rectangular locking projections 55 on the opposite sides of the frame on the opposite side of the case entrance and exit 25 of the rack 2 and on the rear side of the front and rear surfaces of the case 1, respectively. In the case where the groove 56 is formed, when the case 1 is pushed through the entrance 25 of the rack 2, the rear part of the case 1 is the case entrance 25 of the rack 2. When the opposite frame is reached, the locking projections 55 formed on the front and rear surfaces of the frame are forcibly fitted into the rectangular locking grooves 56 formed on both rear sides of the rear frame. The state fixed in the storage space part of () is maintained.

Therefore, the case 1 may be easily prevented from being separated from the rack 2 during transport and handling, thereby completely preventing contamination between samples, difficulty in handling, and damage during transport and handling.

On the other hand, when you want to remove any one of the several cases (1) stored and moved in the rack (2) from the rack, if you pull only the case (1) strongly toward the entrance 25 side of the case (1) Since the frame locking protrusion 55 inserted into the rectangular locking groove 56 is pulled out of the locking groove 56 of the case 1 forcibly pulled out, only a desired case can be removed from the storage space of the rack 2.

Of course, at this time, even if the rack 2 is inclined toward the doorway due to carelessness of the operator when forcibly pulling out only one of the several cases containing the sample, the locking groove 56 of the other cases (1) Since it keeps caught in the locking part 55 of the frame, it is possible to completely prevent contamination or handling difficulties between samples due to automatic detachment from the rack when the desired case is taken out, and damage during transportation and handling. It is.

As described above, according to the present invention, the case is fixed and freed by units by installing fixing means on the case entrance or opposite side of the rack, which is a holder for storing a biological sample, which is used for a vessel for cryopreserving a biological sample. By allowing the number of cases stored in the rack to be controlled through separate fastening means, it is possible to prevent them from being easily detached from the rack during transportation and handling, as well as other cases when withdrawing the desired case. Cases are also very useful designs to completely prevent contamination between samples due to detachment from the rack, difficulty in handling, and damage during transport and handling.

Claims (4)

Several cases (1) comprising a body (11) for accommodating predetermined contents and a cover (12) for covering the opening of the body (11); As a long cube, one side has a frame 21 which is open to allow the case 1 to enter and exit along a corner, and has a left and right separation prevention jaw 222 and is partitioned to a height at which the case 1 can be slid and stored. A rack (2) comprising several partitions (22) and a handle (23) attached to an upper surface of the elongated cube; In the holder for cryogenic cryopreservation vessel composed of fixing means (5) to prevent the case (1) stored in the space portion of the rack (2) partitioned by the partition (22) from being separated from the rack (2) , The fixing means 5 is installed and formed separately at the case entrance 25 of the rack 2 or the opposite side of the entrance 25 so that the cases 1 can be fixed and released by units. Out-of-case protection against racks for cryogenic cryopreservation vessel holders. The method according to claim 1, The fixing means (5), the upper end of the long cover 51 formed to be bent so as to have a cross-section "c" shape axially to the upper portion of the frame 21 on each case entrance 25 side of the rack (2), A pair of locking protrusions 52 are formed at a lower end of the cover 51, and a locking hole into which the locking protrusions 52 are fitted to the lower ends of the frame 21 at each case entrance and exit side 25 of the rack 2 ( 53) Case separation prevention device in the rack for cryogenic cryopreservation vessel holder, characterized in that perforated. The method according to claim 1, The fixing means 5 has one or two leaf springs 54 formed by bending a steel plate having a good tension in an approximately " 형상 " shape. A case detachment preventing device in a rack for cryogenic cryopreservation vessel holder, which is fixedly installed on both the upper surface or the upper surface of the base. The method according to claim 1, The fixing means 5 is a rectangular locking protrusion 55 and a locking groove 56 on both sides of the frame 21 opposite the case entrance and exit 25 of the rack 2 and on the rear side of the front and rear surfaces of the case 1, respectively. The case departure prevention device in the rack for cryogenic cryopreservation vessel holder, characterized in that formed to face each other.