CN216567932U - Cell cryopreserving box - Google Patents

Abstract

The utility model discloses a cell freezing storage box, which comprises a shell, wherein the shell comprises an upper cover and a box body, and the upper cover is movably connected with the box body; the cooling assembly is positioned in the box body and comprises a first medium block and a second medium block, a plurality of accommodating cavities are formed in the first medium block, and a plurality of inserting holes are formed in the second medium block in a penetrating manner; still include lifting unit, lifting unit is located first medium piece top, and lifting unit includes the bottom plate, and the bottom plate rotates and is connected with two bracing pieces, and two bracing piece sliding connection have the cryopreserved pipe support, rotate on two bracing pieces and be connected with the pull rod, and pull rod one end rigid coupling has the acting as go-between, and the other end and the upper cover rigid coupling of acting as go-between freeze and deposit and be equipped with a plurality of pipe clamps on the pipe support, cryopreserved pipe support four corners sliding connection has first spout. This device can cover the cryopreserving pipe in the cell cryopreserving box completely, improves the stability of cell cooling.

Description

Cell cryopreserving box

Technical Field

The utility model relates to a cell cryopreserving technical field especially relates to a cell cryopreserving box.

Background

Cell cryopreservation is a technique for storing cells in a low-temperature environment to reduce cell metabolism and facilitate long-term storage. The cell freezing needs to use a cell freezing box, the freezing rate of the cell freezing box can reach-1 ℃/minute, the cells are firstly put into a freezing tube, then the freezing tube is put into the cell freezing box, and the cell freezing box is put into a refrigerator at-80 ℃.

Usually will freeze and deposit the pipe and put into the cell and freeze in the cooling medium that the box was opened and has the tube hole in, still can leave the upper portion and freeze and deposit the pipe outside the tube hole, this part freezes and deposits the pipe and need the lid cooperation to cover it, need aim at when covering and cover the hole of cooling medium, and the operation process is inconvenient, and a lot of be fit for in addition and cover also not have the cooling medium, can lead to the cell cooling process too fast, can not reach required requirement.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a cell cryopreserved box to solve the problem that above-mentioned prior art exists, make and cryopreserved pipe access convenient, enable to cryopreserved pipe moreover and be in inside the cooling medium completely, prevent that the cell cooling of cryopreserved is too fast.

In order to achieve the above object, the utility model provides a following scheme: the utility model provides a cell cryopreserving box, include:

the shell comprises an upper cover and a box body, the upper cover is movably connected with the box body, and a third dielectric block is embedded in the upper cover;

the cooling component is positioned in the box body and comprises a first medium block and a second medium block, a plurality of accommodating cavities are formed in the first medium block, a plurality of jacks are formed in the second medium block in a penetrating manner, and the jacks are arranged corresponding to the accommodating cavities;

the lifting assembly is located at the top of the first medium block, the lifting assembly comprises a bottom plate, the bottom plate is connected with two supporting rods in a rotating mode, the two supporting rods are connected with a cryopreserved pipe support in a sliding mode, the two supporting rods are connected with a pull rod in a rotating mode, one end of the pull rod is fixedly connected with a pull wire, the other end of the pull wire is fixedly connected with an upper cover, a plurality of pipe clamps are arranged on the cryopreserved pipe support, the pipe clamps correspond to the containing cavities, the cryopreserved pipe support is located at the bottom of the second medium block, and four corners of the cryopreserved pipe support are connected with first sliding grooves in a sliding mode.

Preferably, the cryopreserved pipe support runs through and has seted up a plurality of through-holes, the through-hole lateral wall runs through and has seted up the fixed orifices, the pipe clamp inner wall with cryopreserved pipe support outer wall looks adaptation, pipe clamp lateral wall rigid coupling connecting rod, the connecting rod is worn to establish in the fixed orifices, the connecting rod is kept away from pipe clamp one end rigid coupling has the dog, the dog size is less than the fixed orifices size, the connecting rod overcoat is equipped with the spring, the spring both ends respectively the butt be in the pipe clamp with on the through-hole lateral wall.

Preferably, the pipe clamps are arranged in the through holes at intervals, and the distance between every two adjacent pipe clamps in the through holes is equal.

Preferably, the four corners of the freezing storage pipe support are fixedly connected with sliding blocks, the sliding blocks slide in the first sliding grooves, and the first sliding grooves are fixedly connected with the bottom plate.

Preferably, the pull rod is located between the bottom plate and the freezing pipe support, and the support rods are arranged in parallel.

Preferably, bracing piece one end is rotated and is connected with first pivot, two mounting holes have been seted up on the bottom plate, first pivot is worn to establish in the mounting hole, first pivot passes through the mounting hole with the bottom plate is rotated and is connected, the bracing piece other end is rotated and is connected with the second pivot, it has seted up the second spout on the two opposite lateral walls of deposit pipe support to freeze, the second pivot is located in the second spout, the second pivot passes through the second spout with it manages support sliding connection to freeze, it is connected with the third pivot to rotate on the bracing piece, the third pivot is worn to establish on the pull rod, the third pivot is located first pivot with between the second pivot.

Preferably, a stay wire pipe penetrates through the second medium block, the stay wire penetrates through the stay wire pipe, four third medium blocks are abutted to the periphery of the first medium block, and the third medium blocks are located at the bottom of the second medium block.

Preferably, the upper cover with the box body passes through the hinge and rotates the connection, the upper cover top rigid coupling handle, the upper cover with the rigid coupling has the hasp lock on the box body, the rigid coupling has the stopper on the box body lateral wall, the stopper with the hinge is at same lateral wall.

The utility model discloses a following technological effect: the device is characterized in that a first medium block is arranged in a box body, a containing cavity on the first medium block is used for storing a cryopreservation tube, the lower half part of the cryopreservation tube is completely positioned in the first medium block, a lifting assembly is used for lifting or putting down the cryopreservation tube, a pull wire can be pulled after a cover body is opened, the pull wire can pull a pull rod, the pull rod can drive two support rods to rotate on a bottom plate, the other ends of the support rods slide on a cryopreservation tube support, the cryopreservation tube support slides in a first sliding chute and rises, the cryopreservation tube is inserted into a jack, the cryopreservation tube can enter a tube clamp, the cell cryopreservation tube is pushed downwards to a proper position, an upper cover is buckled, the pull wire can be loosened during buckling, the pull rod can lose the tensile force, the support rods can not have the supporting force any more, the cryopreservation tube support can fall down, the lower half part of the cell cryopreservation tube falls into the containing cavity, and the exposed part of the cell cryopreservation tube can also completely enter a second medium block, and the third medium block in the upper cover is matched, so that the process of cooling the cells is more stable.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor.

FIG. 1 is a schematic structural view of the cell cryopreservation box of the present invention;

FIG. 2 is a partial enlarged view of a in FIG. 1;

fig. 3 is a top cross-sectional view of the first chute of the present invention;

FIG. 4 is a schematic view of the structure of the freezing storage tube support of the present invention;

FIG. 5 is a partial enlarged view of b in FIG. 4;

FIG. 6 is a schematic diagram of a second media block structure;

wherein, 1, an upper cover; 2. a box body; 3. a first dielectric block; 4. a second dielectric block; 5. an accommodating chamber; 6. a jack; 7. a base plate; 8. a hasp lock; 9. a limiting block; 10. a support bar; 11. freezing and storing the tube support; 12. a pull rod; 13. pulling a wire; 14. a pipe clamp; 15. a first chute; 16. a through hole; 17. a connecting rod; 18. a stopper; 19. a spring; 20. a slider; 21. a first rotating shaft; 22. a second rotating shaft; 23. a second chute; 24. a stay wire tube; 25. a fourth dielectric block; 26. a third dielectric block; 27. a hinge; 28. a handle; 29. and a third rotating shaft.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

Referring to fig. 1-6, the utility model provides a cell cryopreservation box, include:

the casing, the casing includes upper cover 1 and box body 2, upper cover 1 and box body 2 are connected movably, the embedded third medium block 26 that is equipped with of upper cover 1; the inside cell that needs to freeze and deposit that can be better after upper cover 1 and 2 locks of box body, third medium piece 26 increases the stability of this device cooling.

The temperature reduction assembly is positioned in the box body 2 and comprises a first medium block 3 and a second medium block 4, a plurality of accommodating cavities 5 are formed in the first medium block 3, a plurality of inserting holes 6 are formed in the second medium block 4 in a penetrating manner, and the inserting holes 6 are arranged corresponding to the accommodating cavities 5; the cell cryopreservation tube is used for storing cells in the accommodating cavity 5 and penetrates through the jack 6 to enter the accommodating cavity 5, the first medium block 3 and the second medium block 4 can slowly cool the cells to be cryopreserved, the temperature is reduced by 1 ℃ per minute under the general condition, in practical use, alcohol liquids such as isopropyl alcohol can be added into the first medium block 3 and the second medium block 4, the first medium block 3 and the second medium block 4 can also use polyethylene foam materials, although the first medium block 3 and the second medium block 4 in the device can be in a closed state, the defects that the isopropyl alcohol is toxic, volatile, inflammable and the like exist, and if misoperation causes leakage, the damage to the health of a human body can be caused, and the polyethylene foam materials are preferably selected in the embodiment.

Lifting unit, lifting unit is located 3 tops of first medium piece, lifting unit includes bottom plate 7, bottom plate 7 rotates and is connected with two bracing pieces 10, two bracing pieces 10 sliding connection have cryopreserved pipe support 11, it is connected with pull rod 12 to rotate on two bracing pieces 10, 12 one end rigid couplings of pull rod have 13 to act as go-between, 13 other ends of acting as go-between and the 1 rigid coupling of upper cover, cryopreserve and be equipped with a plurality of pipe clamps 14 on the pipe support 11, pipe clamp 14 corresponds the setting with holding chamber 5, cryopreserved pipe support 11 is located 4 bottoms of second medium piece, cryopreserved pipe support 11 four corners sliding connection has first spout 15.

When opening upper cover 1, can stimulate 13 acting as go-between, 13 acting as go-between can stimulate pull rod 12, and pull rod 12 can drive two bracing pieces 10 and rotate on bottom plate 7, and the other end of two bracing pieces 10 can slide on freezing a tub support 11 this moment, freezes and deposits tub support 11 under the effect of first spout 15, can upward movement. The cryopreservation tube bracket 11 is used for storing cell cryopreservation tubes, the tube clamps 14 can clamp the cell cryopreservation tubes, the tube clamps 14 are usually used in pairs, when in actual use, the cell freezing tube is inserted into the jack 6 of the first medium block 3, the aperture size of the jack 6 is larger than the diameter of the cell freezing tube, the cell freezing tube can not be blocked, the cell freezing tube can be fixed in the tube clamp 14, when the cell freezing tube is completely stored, the upper cover 1 is buckled, when the cell freezing tube is buckled, the pull wire 13 is loosened, the pull rod 12 loses the pulling force, so that the support rod 10 has no supporting force any more, the freezing tube support 11 falls down, the lower half part of the cell freezing tube falls into the accommodating cavity 5, and the first half of the cell cryopreserving pipe also can all enter into second medium piece 4 this moment, conveniently preserves the cell cryopreserving pipe, and lifting unit sets up to two sets of in practical application preferentially.

According to the further optimized scheme, a plurality of through holes 16 are formed in the freezing tube support 11 in a penetrating mode, fixing holes are formed in the side wall of each through hole 16 in a penetrating mode, the inner wall of the tube clamp 14 is matched with the outer wall of the freezing tube support 11, the outer side wall of the tube clamp 14 is fixedly connected with a connecting rod 17, the connecting rod 17 is arranged in each fixing hole in a penetrating mode, one end, far away from the tube clamp 14, of the connecting rod 17 is fixedly connected with a stop block 18, the size of the stop block 18 is smaller than that of each fixing hole, a spring 19 is sleeved outside the connecting rod 17, and two ends of the spring 19 abut against the tube clamp 14 and the side wall of the through hole 16 respectively; the connecting rod 17 penetrates through the fixing hole to enable the pipe clamp 14 to be kept at the same height, the stop block 18 prevents the connecting rod 17 from being separated from the fixing hole, and under the action of the spring 19, the pipe clamp 14 can keep elasticity when clamping the cell cryopreservation pipe, so that the cell cryopreservation pipe can conveniently move up and down.

According to the further optimized scheme, the pipe clamps 14 are arranged in the through holes 16 at intervals, and the distance between every two adjacent pipe clamps 14 in the through holes 16 is equal; the tube clamps 14 are arranged in a row in the through holes 16, and an empty through hole 16 is formed between the two rows of the tube clamps 14, so that the cell freezing tube can be conveniently taken and placed.

According to a further optimized scheme, four corners of the freezing storage pipe support 11 are fixedly connected with sliding blocks 20, the sliding blocks 20 slide in the first sliding grooves 15, and the first sliding grooves 15 are fixedly connected with the bottom plate 7; the freezing pipe support 11 is connected with the first sliding groove 15 in a sliding mode through the sliding block 20, and the freezing pipe support 11 is convenient to move up and down.

Further optimize the scheme, pull rod 12 is located between bottom plate 7 and the cryopreserving pipe support 11, and bracing piece 10 parallel arrangement, pull rod 12 can be more laborsaving during the bracing piece 10 of pulling parallel arrangement, and in practical application, bracing piece 10 pivoted angle can not rotate vertical direction, only can move in level and vertical contained angle.

According to a further optimization scheme, one end of a support rod 10 is rotatably connected with a first rotating shaft 21, two mounting holes are formed in a bottom plate 7, the first rotating shaft 21 penetrates through the mounting holes, the first rotating shaft 21 is rotatably connected with the bottom plate 7 through the mounting holes, the other end of the support rod 10 is rotatably connected with a second rotating shaft 22, two opposite side walls of a cryopreservation tube support 11 are provided with second sliding grooves 23, the second rotating shaft 22 is located in the second sliding grooves 23, the second rotating shaft 22 is slidably connected with the cryopreservation tube support 11 through the second sliding grooves 23, a third rotating shaft 29 is rotatably connected to the support rod 10, the third rotating shaft 29 penetrates through a pull rod 12, and the third rotating shaft 29 is located between the first rotating shaft 21 and the second rotating shaft 22; the bracing piece 10 rotates through first pivot 21 and mounting hole and bottom plate 7 to be connected, makes the bracing piece 10 more firm when rotating, and the bracing piece 10 slides in second spout 23 through second pivot 22, and when the bracing piece 10 was pulled to pull rod 12, when the bracing piece 10 rotated around first pivot 21, can slide in second spout 23 to the messenger freezes deposits a tub support 11 and reciprocates.

According to the further optimized scheme, the stay wire pipe 24 penetrates through the second medium block 4, the stay wire 13 penetrates through the stay wire pipe 24, four fourth medium blocks 25 are abutted to the periphery of the first medium block 3, and the fourth medium blocks 25 are located at the bottom of the second medium block 4; the stay wire 13 is arranged in the stay wire pipe 24 to prevent the second medium block 4 from being damaged, and the four fourth medium blocks 25 around the first medium block 3 increase the heat preservation effect of the device, so that the device is cooled more stably.

According to a further optimized scheme, the upper cover 1 is rotatably connected with the box body 2 through a hinge 27, the top end of the upper cover 1 is fixedly connected with a handle 28, the upper cover 1 and the box body 2 are fixedly connected with a hasp lock 8, the side wall of the box body 2 is fixedly connected with a limiting block 9, and the limiting block 9 and the hinge 27 are arranged on the same side wall; this device of conveniently taking of handle 28, hasp 8 makes things convenient for this device locking and opens, freezes at-80 ℃ and deposits the back, adopts the screw thread to screw closed device and can be difficult more open, and long-time contact easily causes staff's frostbite moreover, and it is all very convenient to adopt hasp 8 to open the closure, and stopper 9 prevents that upper cover 1 from opening excessively, causes and draws line 13 excessively to pull, damages lifting unit, and hasp 8 is prior art, no longer gives unnecessary details.

The using method of the device comprises the steps that the cell cryopreservation tube is inserted into the jack 6 of the second medium block 4, the tube clamp 14 can clamp the inserted cell cryopreservation tube, the cell cryopreservation tube is pushed downwards to a proper position, the part of the second medium block 4 exposed can completely enter the second medium block 4 after the upper cover 1 is buckled, after the cell cryopreservation tube is placed, the upper cover 1 is buckled, the pull wire 13 can be loosened during buckling, the pull rod 12 can also be loosened, the support rod 10 loses the supporting force, the cryopreservation tube support 11 clamping the cell cryopreservation tube falls down along the first sliding groove 15, after the cryopreservation tube support 11 falls down, the lower half part of the cell cryopreservation tube can enter the first medium block 3, and after the upper cover 1 is buckled, the device is locked and placed in a refrigerator through the buckling lock 8 to cool. When this device is opened to needs, open earlier hasp lock 8, lift upper cover 1, stopper 9 can prevent that upper cover 1 from opening excessively, and the stay wire 13 can be taut, and stay wire 13 stimulates pull rod 12, and pull rod 12 drives the bracing piece 10 and rotates, and the second pivot 22 of bracing piece 10 slides in the second spout 23 of freezing a tub support 11, thereby makes freezing a tub support 11 and upwards removes, freezes the pipe clamp 14 on the tub support 11 and also can drive the cell and freeze a tub rebound, exposes second medium piece 4, conveniently takes.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-mentioned embodiments are only intended to describe the preferred embodiments of the present invention, but not to limit the scope of the present invention, and those skilled in the art should also be able to make various modifications and improvements to the technical solution of the present invention without departing from the spirit of the present invention, and all such modifications and improvements are intended to fall within the scope of the present invention as defined in the appended claims.

Claims (8)

1. A cell cryopreservation cartridge comprising:

the shell comprises an upper cover (1) and a box body (2), the upper cover (1) is movably connected with the box body (2), and a third medium block (26) is embedded in the upper cover (1);

the cooling component is positioned in the box body (2), the cooling component comprises a first medium block (3) and a second medium block (4), a plurality of accommodating cavities (5) are formed in the first medium block (3), a plurality of jacks (6) are formed in the second medium block (4) in a penetrating manner, and the jacks (6) are arranged corresponding to the accommodating cavities (5);

lifting unit, lifting unit is located first medium piece (3) top, lifting unit includes bottom plate (7), bottom plate (7) rotate and are connected with two bracing pieces (10), two bracing piece (10) sliding connection freezes deposits tub of support (11), two rotate on bracing piece (10) and be connected with pull rod (12), pull rod (12) one end rigid coupling has stay wire (13), the stay wire (13) other end with upper cover (1) rigid coupling, it is equipped with a plurality of pipe clamps (14) on depositing tub of support (11) to freeze, pipe clamp (14) with it corresponds the setting to hold chamber (5), it is located to freeze tub of support (11) second medium piece (4) bottom, it has first spout (15) to freeze tub of support (11) four corners sliding connection.

2. The cell cryopreservation cassette of claim 1, wherein: cryopreserved pipe support (11) run through and seted up a plurality of through-holes (16), through-hole (16) lateral wall runs through and has seted up the fixed orifices, pipe clamp (14) inner wall with cryopreserved pipe support (11) outer wall looks adaptation, pipe clamp (14) lateral wall rigid coupling connecting rod (17), connecting rod (17) are worn to establish in the fixed orifices, connecting rod (17) are kept away from pipe clamp (14) one end rigid coupling has dog (18), dog (18) size is less than the fixed orifices size, connecting rod (17) overcoat is equipped with spring (19), spring (19) both ends butt respectively are in pipe clamp (14) with on through-hole (16) lateral wall.

3. The cell cryopreservation cassette of claim 2, wherein: the pipe clamps (14) are arranged in the through holes (16) at intervals, and the distance between every two adjacent pipe clamps (14) in the through holes (16) is equal.

4. The cell cryopreservation cassette of claim 1, wherein: freezing pipe support (11) four corners rigid coupling has slider (20), slider (20) are in slide in first spout (15), first spout (15) with bottom plate (7) rigid coupling.

5. The cell cryopreservation cassette of claim 1, wherein: the pull rod (12) is located between the bottom plate (7) and the freezing storage pipe support (11), and the support rods (10) are arranged in parallel.

6. The cell cryopreservation cassette of claim 1, wherein: one end of the supporting rod (10) is rotatably connected with a first rotating shaft (21), the bottom plate (7) is provided with two mounting holes, the first rotating shaft (21) penetrates through the mounting hole, the first rotating shaft (21) is rotatably connected with the bottom plate (7) through the mounting hole, the other end of the support rod (10) is rotatably connected with a second rotating shaft (22), two opposite side walls of the freezing tube bracket (11) are provided with second sliding grooves (23), the second rotating shaft (22) is positioned in the second sliding groove (23), the second rotating shaft (22) is in sliding connection with the freezing pipe support (11) through the second sliding groove (23), the support rod (10) is rotatably connected with a third rotating shaft (29), the third rotating shaft (29) is arranged on the pull rod (12) in a penetrating way, the third rotating shaft (29) is positioned between the first rotating shaft (21) and the second rotating shaft (22).

7. The cell cryopreservation cassette of claim 1, wherein: wear to be equipped with in second dielectric block (4) and act as go-between pipe (24), act as go-between (13) are worn to establish in act as go-between pipe (24), first dielectric block (3) butt joint has four fourth dielectric blocks (25) all around, fourth dielectric block (25) are located second dielectric block (4) bottom.

8. The cell cryopreservation cassette of claim 1 wherein: upper cover (1) with box body (2) rotate through hinge (27) and are connected, upper cover (1) top rigid coupling handle (28), upper cover (1) with the rigid coupling has hasp lock (8) on box body (2), the rigid coupling has stopper (9) on box body (2) lateral wall, stopper (9) with hinge (27) are at same lateral wall.

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