CN215958000U - Cell liquid nitrogen freezes deposits uses storage container - Google Patents
Cell liquid nitrogen freezes deposits uses storage container Download PDFInfo
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- CN215958000U CN215958000U CN202122396347.0U CN202122396347U CN215958000U CN 215958000 U CN215958000 U CN 215958000U CN 202122396347 U CN202122396347 U CN 202122396347U CN 215958000 U CN215958000 U CN 215958000U
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 118
- 239000007788 liquid Substances 0.000 title claims abstract description 64
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 59
- 238000003860 storage Methods 0.000 title claims abstract description 31
- 238000007710 freezing Methods 0.000 claims abstract description 105
- 230000008014 freezing Effects 0.000 claims abstract description 105
- 230000007246 mechanism Effects 0.000 claims abstract description 52
- 238000005138 cryopreservation Methods 0.000 claims abstract description 46
- 238000003825 pressing Methods 0.000 claims abstract description 24
- 238000004321 preservation Methods 0.000 claims abstract description 15
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 13
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 13
- 241001330002 Bambuseae Species 0.000 claims description 13
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 13
- 239000011425 bamboo Substances 0.000 claims description 13
- 238000005057 refrigeration Methods 0.000 claims description 5
- 238000005056 compaction Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 210000004027 cell Anatomy 0.000 description 31
- 238000000151 deposition Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 6
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- 238000013461 design Methods 0.000 description 3
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- 210000001503 joint Anatomy 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 1
- 238000011001 backwashing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
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- 238000002309 gasification Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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Abstract
The utility model discloses a cell liquid nitrogen cryopreservation preservation container, which comprises a cryopreservation tube, at least one cryopreservation box for bearing the cryopreservation tube and a liquid nitrogen biological container for storing the cryopreservation box, wherein the cryopreservation box comprises a plate body, accommodating tubes and a pressing mechanism, the accommodating tubes are arranged on the lower surface of the plate body, and the pressing mechanism is arranged on the upper surface of the plate body and is used for pressing the cryopreservation tubes in the accommodating tubes; the accommodating pipe is a grid pipe; the plurality of freezing storage boxes are provided with codes according to the numerical sequence; the compressing mechanism comprises an L-shaped rod, a telescopic rod and a pressing sheet, the L-shaped rod is rotatably arranged on the upper surface of the plate body, the pressing sheet is arranged on the L-shaped rod through the telescopic rod, and the pressing sheet is arranged opposite to the freezing tube; the plate body is an elliptical plate, a ring groove is formed in the side face of the elliptical plate, and a bearing clamping mechanism matched with the ring groove is arranged in a refrigerating cavity of the liquid nitrogen biological container. The preservation container improves operability and freezing effect, has uniform freezing temperature, reduces the occurrence of the phenomenon of ice plug in the freezing storage pipe, and has good freezing effect.
Description
Technical Field
The utility model relates to the technical field of cell cryopreservation containers, in particular to a preservation container for cell liquid nitrogen cryopreservation.
Background
In the process of passage and daily maintenance of cell culture, a great deal of cost is needed in culture instruments, culture solutions and various preparation works, and once the cells leave a living body and start primary culture, various biological characteristics of the cells gradually change and continuously and newly change along with the increase of the passage times and the change of in vitro environmental conditions. Therefore, it is necessary to freeze and store the cells in time. The cells can be stored for one year in a refrigerator at-70 ℃ after being frozen; the cells were stored in liquid nitrogen at a temperature of-196 ℃ for a theoretically unlimited storage time. Cell cryopreservation is a technique for placing cells in a low-temperature environment, reducing cell metabolism and facilitating long-term storage. Cell cryopreservation is one of the main methods for cell preservation, and plays a role in cell seed preservation. The cells are stored in liquid nitrogen at the temperature of-196 ℃ by using a freezing technology at a low temperature, so that the cells can be temporarily separated from a growth state and the cell characteristics can be stored, and the cells are recovered for experiments when needed. Moreover, a certain amount of cells are preserved appropriately, so that the cells can be prevented from being lost due to contamination or other accidents of the cultured cells, and the function of preserving the cells is achieved.
The cell freezing container is basically a liquid nitrogen tank, and when the freezing tube is used for storing samples, the freezing tube is strictly required to be placed in a gas phase layer of liquid nitrogen. According to the conventional freezing box for containing the freezing tubes in the liquid nitrogen tank, on one hand, the structure of the freezing box is integrated, and when the freezing box is taken out and put down, the freezing box can be integrally taken out of the liquid nitrogen tank every time the freezing tubes are taken out, so that the operation is inconvenient and labor is wasted, and the rest freezing tubes are easily influenced by external temperature; on the other hand, if staff's misoperation, fill into liquid nitrogen in the liquid nitrogen cylinder too much, when leading to the liquid level of liquid nitrogen too high, either take out partial liquid nitrogen, or freeze and deposit the box and can inevitablely store the cryopreserved pipe in liquid nitrogen liquid, the liquid nitrogen has the probability to permeate and freezes and deposit intraduct, and liquid nitrogen gasification leads to inside and outside pressure imbalance during the recovery, easily causes the burst of cryopreserved pipe like this to have biohazard. During the process of storing the frozen cells, the freezing temperature of the freezing tube needs to be uniform. The asymmetric freezing will result in the creation of a plug that will inhibit the transfer of liquid temperatures from both sides and thereby create dangerously high pressures and damage to the vial.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a cell liquid nitrogen cryopreservation container, which solves the problems that a cryopreservation box in a traditional liquid nitrogen tank is inconvenient to use, freezing temperature of a cryopreservation tube is not uniform, so that ice plugs can be generated due to non-uniform freezing, and the ice plugs can inhibit liquid temperature transmission at two sides to further generate critical high pressure and damage the cryopreservation tube.
In order to solve the technical problems, the utility model adopts the following technical scheme:
a cell liquid nitrogen cryopreservation preservation container comprises a cryopreservation tube, at least one cryopreservation box used for bearing the cryopreservation tube, and a liquid nitrogen biological container used for storing the cryopreservation box, wherein the cryopreservation box comprises a plate body, a containing tube and a pressing mechanism, the containing tubes are uniformly arranged and vertically installed on the lower surface of the plate body, and the pressing mechanism is installed on the upper surface of the plate body and tightly supports the cryopreservation tube in the containing tube; wherein,
the accommodating pipe is a grid pipe;
the plurality of freezing storage boxes are provided with codes according to the numerical sequence;
the compaction mechanism comprises an L-shaped rod, a telescopic rod and a pressing sheet, the L-shaped rod is rotatably arranged on the upper surface of the plate body, the pressing sheet is arranged on the L-shaped rod through the telescopic rod, and the pressing sheet is arranged opposite to the freezing tube;
the plate body is an elliptical plate, an annular groove is formed in the side face of the elliptical plate, and a bearing clamping mechanism matched with the annular groove is arranged in a refrigerating cavity of the liquid nitrogen biological container.
The further technical scheme is as follows: it is adjacent freeze and deposit and be provided with latch mechanism between the box, latch mechanism includes pothook and stop block, the annular symmetry sets up the both sides face of plate body, the tip of pothook rotates to be installed the both sides face of plate body, just the rotation end of pothook is located the annular below, the stop block sets up respectively the annular with on the fitting surface of pothook, one freeze and deposit on the box the pothook passes through the stop block is adjacent with another freeze and deposit on the box the annular joint.
The further technical scheme is as follows: bearing clamping mechanism includes first spring, slide bar and location ball piece, the slide bar passes through first spring symmetry sets up and slidable mounting in the recess on the cold-stored intracavity lateral wall, the location ball piece is installed the outer end of slide bar, the inboard of annular be provided with location ball piece assorted spherical sunken.
The further technical scheme is as follows: adjacent be provided with positioning mechanism between the freezing box, positioning mechanism includes reference column, a location section of thick bamboo, second spring and sliding plate, the reference column symmetry sets up and vertically installs the lower surface of plate body, a location section of thick bamboo symmetry sets up and vertically installs the upper surface of plate body, the sliding plate passes through second spring slidable mounting in the location section of thick bamboo, one on the freezing box the reference column is adjacent with another on the freezing box a location section of thick bamboo sliding fit.
The further technical scheme is as follows: and a cone is arranged at one end of the positioning column, and a taper sleeve matched with the cone is arranged on the sliding plate.
The further technical scheme is as follows: two positioning columns on two sides of the plate body are respectively a cylinder and a prism.
The further technical scheme is as follows: the diameter of the ring groove is consistent with the minor axis of the elliptical plate.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model provides a cell liquid nitrogen cryopreservation storage container, which utilizes a liquid nitrogen biological container and a cryopreservation box matched with the liquid nitrogen biological container, can utilize a bearing clamping mechanism between the cryopreservation box and the liquid nitrogen biological container to facilitate the installation of the cryopreservation box when a cryopreservation tube is stored and taken out, can realize the butt joint of a new cryopreservation box bearing the cryopreservation tube and the cryopreserved cryopreservation box on the premise of not completely taking out the cryopreserved cryopreservation box, and on the contrary, does not need to take out the whole cryopreservation box every time when the cryopreservation tube is taken out, thereby avoiding the problem of influencing other cryopreservation tubes, and improving the operability and cryopreservation effect of the cell liquid nitrogen cryopreservation storage container.
2. When depositing the cell with the liquid nitrogen container freezing, utilize and freeze and deposit the box and hold and freeze and deposit the pipe, it is the net pipe to hold the pipe, and cancellation closed box body structure, guarantee liquid nitrogen and freeze the even contact who deposits the pipe, freezing temperature is even, reduce and freeze the emergence of depositing intraductal ice plug phenomenon, should freeze simultaneously and deposit the box and can superpose each other, deposit or take out and freeze when depositing the pipe, need not take out all freezing and deposit the box completely at every turn, it is laborsaving convenient, only with lifting up appointed freezing and depositing the box and pull down, below it need not expose outside, reduce the influence of ambient temperature, and independent freezing and depositing box convenient to use back washing, and the stack design of freezing and depositing the box, when avoiding tradition to freeze and deposit, the loaded down with trivial details operation of big box cover box, should freeze and deposit pipe protection component, convenient to use, freezing is effectual.
3. In addition, the pressing mechanism on the freezing storage pipe protection assembly can be suitable for mounting and pressing freezing storage pipes of various specifications.
Drawings
FIG. 1 is a schematic structural diagram of a preservation container for freezing and storing cells in liquid nitrogen according to the present invention.
Figure 2 is a schematic view of the present invention load bearing clamp mechanism of figure 1.
FIG. 3 is a schematic structural view of the freezing storage boxes in FIG. 1 clamped with each other according to the present invention.
FIG. 4 is a schematic structural diagram of a single cryopreservation box in FIG. 3 according to the utility model.
Fig. 5 is a schematic structural diagram of the plate body in the utility model 1.
Reference numerals: 1. freezing and storing the tube; 2. freezing and storing the box; 3. a liquid nitrogen biocontainer; 31. a refrigerated chamber; 311. a groove; 4. a plate body; 41. a ring groove; 411. spherical depressions; 5. accommodating the tube; 6. a hold-down mechanism; 61. an L-shaped rod; 62. a telescopic rod; 63. tabletting; 7. a clamping mechanism; 72. a hook; 73. a stopper block; 8. a positioning mechanism; 81. a positioning column; 811. a cone; 82. a positioning cylinder; 83. a second spring; 84. a sliding plate; 841. a taper sleeve; 9. a load bearing clamping mechanism; 91. a first spring; 92. a slide bar; 93. positioning the ball block; 10. and (5) encoding.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when the products of the present invention are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are used only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element that is referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The first embodiment is as follows:
in this example, as shown in FIGS. 1, 2, 3 and 5, a preservation container for freezing cells in liquid nitrogen is constructed as follows: the freezing and storing device comprises freezing and storing tubes 1 (both internal rotation type and external rotation type freezing and storing tubes), at least one freezing and storing box 2 used for bearing the freezing and storing tubes 1 and a liquid nitrogen biological container 3 (YDS-10 liquid nitrogen tank can be adopted) used for storing the freezing and storing box 2, wherein the freezing and storing box 2 comprises a plate body 4, accommodating tubes 5 and a pressing mechanism 6, a plurality of accommodating tubes 5 are uniformly arranged and vertically arranged on the lower surface of the plate body 4, the plate body 4 is also provided with mounting holes which are communicated with the accommodating tubes 5 and used for the freezing and storing tubes 1 to pass through, elastic positioning materials (such as rubber rings and the like) can be arranged in the mounting holes, the pressing mechanism 6 is arranged on the upper surface of the plate body 4, and the freezing and storing tubes 1 are tightly propped in the accommodating tubes 5; the accommodating pipe 5 is a grid pipe; the plurality of freezing storage boxes 2 are provided with codes 10 according to the numerical sequence; the pressing mechanism 6 comprises an L-shaped rod 61, an expansion rod 62 and a pressing sheet 63, the L-shaped rod 61 is rotatably arranged on the upper surface of the plate body 4, the pressing sheet 63 is arranged on the L-shaped rod 61 through the expansion rod 62, and the pressing sheet 63 is arranged right opposite to the freezing tube 1; the plate body 4 is an elliptical plate, a ring groove 41 is arranged on the side surface of the elliptical plate, and a bearing clamping mechanism 9 matched with the ring groove 41 is arranged in the refrigeration cavity 31 of the liquid nitrogen biological container 3.
The working process of the utility model is as follows: utilize freezing of this container of liquid nitrogen biological container 3 and adaptation to deposit 2 access freezing and deposit when managing 1, can utilize freezing bearing clamping mechanism 9 between depositing box 2 and the liquid nitrogen biological container 3, make things convenient for the installation of freezing box 2, need take out when freezing box 2, the staff only need rotate plate body 4, bearing clamping mechanism 9 can separate with annular 41 gradually, remove the locking relation, can take out freezing box 2 smoothly, when needs with the new freezing box 2 stack of depositing in order freezing box 2 that deposits, can utilize annular 41, clamping mechanism 7 and positioning mechanism 8 dock adjacent freezing box 2, notice: the ring groove 41 is not interfered with the clamping mechanism 7 and the bearing clamping mechanism 9; before wanting to take out the cryopreserved box 2, lift up cryopreserved box 2 earlier and at rotor plate body 4, this save container is under the cryopreserved box 2 prerequisite of incomplete taking out cryopreserved, can realize docking new cryopreserved box 2 with cryopreserved box 2, otherwise, when taking out the cryopreserved pipe, also need not take out whole cryopreserved box 2 at every turn, influence other problem of cryopreserved pipe 4, and when the liquid level in liquid nitrogen biological container 3 was too high, can reduce the number of piles of cryopreserved box 2, guarantee that every cryopreserved box 2 homoenergetic stores in the gaseous phase of liquid nitrogen biological container 3, need not take out the too much liquid nitrogen in the liquid nitrogen biological container 3, reduce work load, improve this cell cryopreserved with the operability and the cryopreserved effect of save container liquid nitrogen.
The code 10 can help the personnel know the number of layers of the cryopreservation box 2 in the refrigeration cavity 31 of the liquid nitrogen biological container 3, the total height of the cryopreservation box 2 is conveniently controlled, and the liquid phase of the liquid nitrogen is prevented from being contacted.
In addition, when using the liquid nitrogen container to cryopreserve the cell, the staff utilizes freezing box 2 to hold freezing tube 1 earlier, freezing tube 1 is put in holding tube 5, holding tube 5 designs into the net pipe, and cancel closed box structure, the liquid nitrogen circulation of being convenient for, guarantee liquid nitrogen and freezing even contact of depositing the pipe, freezing temperature is even, reduce the emergence of the intraductal plug phenomenon of freezing, in addition, hold the installation and the pressure tightly of the pipe of freezing of multiple specification can be suitable for to the hold by hold down mechanism 6 on the pipe protection component of freezing, place freezing tube 1 before holding tube 5, rotate L type pole 61 earlier, prevent that preforming 63 from plugging up holding tube 5's entry, after freezing tube 1 places holding tube 5, rotate L type pole 61 once more, prevent that preforming 63 from compressing tightly freezing tube 1 in holding tube 5 through the pressure of telescopic link 62 (can be the casing structure of built-in spring). This cryopreserved pipe protection subassembly facilitates the use, and refrigeration effect is good.
Example two:
in this embodiment, as shown in fig. 4, a latch mechanism 7 is disposed between adjacent freezing storage boxes 2, the latch mechanism 7 includes hooks 72 and a stop block 73, the annular grooves 41 are symmetrically disposed on two side surfaces of the plate body 4, end portions of the hooks 72 are rotatably mounted on the two side surfaces of the plate body 4, a rotating end of each hook 72 is located below the annular groove 41, the stop blocks 73 are respectively disposed on matching surfaces of the annular groove 41 and the hooks 72, and the hook 72 on one freezing storage box 2 is latched with the annular groove 41 on another adjacent freezing storage box 2 through the stop block 73.
Be provided with positioning mechanism 8 between the adjacent box 2 that freezes, positioning mechanism 8 includes reference column 81, a location section of thick bamboo 82, second spring 83 and sliding plate 84, and reference column 81 symmetry sets up and vertically installs the lower surface at plate body 4, and a location section of thick bamboo 82 symmetry sets up and vertically installs the upper surface at plate body 4, and sliding plate 84 passes through second spring 83 slidable mounting in a location section of thick bamboo 82, and reference column 81 on one freezes the box 2 and another adjacent location section of thick bamboo 82 sliding fit on freezing the box 2 that freezes.
Meanwhile, the freezing storage boxes 2 can be mutually overlapped through the clamping mechanism 7, the clamping hook 72 on one freezing storage box 2 is clamped with the annular groove 41 on the other adjacent freezing storage box 2 through the stop block 73 to form combined overlapping, when a freezing storage pipe is stored or taken out, all freezing storage boxes do not need to be taken out completely at each time, only the appointed freezing storage box is lifted and detached, the lower part of the freezing storage box is not exposed, the influence of the external temperature is reduced, the independent freezing storage box is convenient to use and clean, in addition, the overlapping design of the freezing storage boxes avoids the complex operation that a large box is sleeved with a small box when the traditional freezing storage is carried out, the freezing storage pipe protection assembly is convenient to use, and the freezing effect is good.
Be provided with positioning mechanism 8 between the adjacent box 2 that freezes, a location section of thick bamboo 82 and reference column 81's sliding fit just provide elasticity, make things convenient for the joint of latch mechanism 7 and improve latch mechanism 7's connection effect.
It is worth noting that: when the hook 72 in the clamping mechanism 7 reaches the horizontal state in the rotating process, the rotating end of the hook 72 can abut against the stop block 73 to reach the maximum rotating amplitude, at this time, the hook 72 in the horizontal state can be prevented from being arranged on the bracket, two supporting ends on the bracket can be mutually matched with the outermost end (the end with the stop block 73) of the hook 72, the stability of the cryopreservation box 2 is ensured, and the cryopreservation tube 1 can be conveniently placed.
Preferably, one end of the positioning post 81 is provided with a cone 811, and the sliding plate 84 is provided with a taper sleeve 841 matched with the cone 811.
The cooperation of the positioning columns 81 and the positioning cylinders 82 and the sliding plate 84 is facilitated.
Preferably, the two positioning columns 81 on the two sides of the plate body 4 are respectively a cylinder and a prism, and the positioning cylinder 82 is changed into a cylinder and a prism by adapting.
When the direction and the butt joint are played, the two positioning columns 81 are respectively a cylinder and a prism, so that a worker can distinguish the correct butt joint position of the freezing storage box 2, and the consistency of the positions of the freezing storage boxes 2 on each layer is ensured.
Preferably, the containment tube 5 is braided from wire.
Preferably, the accommodating tube 5 is formed by hollowing out a metal tube.
Example three:
in the embodiment, the load-bearing clamping mechanism 9 includes a first spring 91, a sliding rod 92 and a positioning ball block 93, the sliding rod 92 is symmetrically disposed through the first spring 91 and slidably mounted in a groove 311 on the inner side wall of the refrigeration cavity 31, the positioning ball block 93 is mounted at the outer end of the sliding rod 92, and a spherical recess 411 matched with the positioning ball block 93 is disposed inside the annular groove 41. The diameter of the ring groove 41 coincides with the minor axis of the elliptical plate.
The clamping hook 72 of the clamping mechanism 7 and the positioning ball block 93 of the bearing clamping mechanism 9 are matched with the annular groove 41, in order to prevent interference, the width of the annular groove 41 needs to be set to be larger than the sum of the thicknesses of the clamping hook 72 and the positioning ball block 93, the cryopreservation box 2 needs to be lifted before the cryopreservation box 2 is taken out by rotation, when rotation is prevented, the positioning ball block 93 collides with the clamping hook 72, in addition, the diameter of the annular groove 41 is consistent with the short axis of the elliptical plate (the diameter of the annular groove 41 is at least larger than or equal to the short axis and smaller than the long axis), and when the cryopreservation box 2 is rotated for a certain angle (the positioning ball block 93 is aligned with or is about to be aligned with the short axis of the elliptical plate), the positioning ball block 93 is separated from the annular groove 41 to realize taking out of the cryopreservation box 2; when the freezing storage box 2 is put into the liquid nitrogen biological container 3, the reverse operation is carried out.
Although the utility model has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, the drawings and the appended claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
Claims (7)
1. A cell liquid nitrogen freezes deposits uses preservation container which characterized in that: the freezing and storing device comprises a freezing and storing pipe (1), at least one freezing and storing box (2) used for bearing the freezing and storing pipe (1) and a liquid nitrogen biological container (3) used for storing the freezing and storing box (2), wherein the freezing and storing box (2) comprises a plate body (4), accommodating pipes (5) and a pressing mechanism (6), the accommodating pipes (5) are uniformly arranged and vertically installed on the lower surface of the plate body (4), the pressing mechanism (6) is installed on the upper surface of the plate body (4), and the freezing and storing pipe (1) is tightly propped in the accommodating pipe (5); wherein,
the accommodating pipe (5) is a grid pipe;
a plurality of the freezing storage boxes (2) are provided with codes (10) according to the numerical sequence;
the compaction mechanism (6) comprises an L-shaped rod (61), an expansion rod (62) and a pressing sheet (63), the L-shaped rod (61) is rotatably installed on the upper surface of the plate body (4), the pressing sheet (63) is installed on the L-shaped rod (61) through the expansion rod (62), and the pressing sheet (63) is arranged opposite to the cryopreservation tube (1);
the plate body (4) is an elliptical plate, an annular groove (41) is formed in the side face of the elliptical plate, and a bearing clamping mechanism (9) matched with the annular groove (41) is arranged in a refrigerating cavity (31) of the liquid nitrogen biological container (3).
2. The preservation container for freezing and storing cell liquid nitrogen according to claim 1, characterized in that: it is adjacent freeze and deposit and be provided with latch mechanism (7) between box (2), latch mechanism (7) include pothook (72) and stop block (73), annular (41) symmetry sets up the both sides face of plate body (4), the tip of pothook (72) is rotated and is installed the both sides face of plate body (4), just the rotation end of pothook (72) is located annular (41) below, stop block (73) set up respectively annular (41) with on the fitting surface of pothook (72), one freeze and deposit on box (2) pothook (72) pass through stop block (73) are adjacent with another freeze and deposit on box (2) annular (41) joint.
3. The preservation container for freezing and storing cell liquid nitrogen according to claim 1, characterized in that: bearing clamping mechanism (9) include first spring (91), slide bar (92) and location ball piece (93), slide bar (92) pass through first spring (91) symmetry sets up and slidable mounting in recess (311) on refrigeration chamber (31) inside wall, location ball piece (93) are installed the outer end of slide bar (92), the inboard of annular (41) be provided with location ball piece (93) assorted spherical sunken (411).
4. The preservation container for freezing and storing cell liquid nitrogen according to claim 1, characterized in that: adjacent freeze and deposit and be provided with positioning mechanism (8) between box (2), positioning mechanism (8) include reference column (81), a location section of thick bamboo (82), second spring (83) and sliding plate (84), reference column (81) symmetry sets up and vertically installs the lower surface of plate body (4), a location section of thick bamboo (82) symmetry sets up and vertically installs the upper surface of plate body (4), sliding plate (84) pass through second spring (83) slidable mounting in a location section of thick bamboo (82), one freeze and deposit on box (2) reference column (81) and another are adjacent freeze and deposit on box (2) a location section of thick bamboo (82) sliding fit.
5. The preservation container for freezing and storing cell liquid nitrogen according to claim 4, characterized in that: one end of the positioning column (81) is provided with a cone (811), and the sliding plate (84) is provided with a taper sleeve (841) matched with the cone (811).
6. The preservation container for freezing and storing cell liquid nitrogen according to claim 5, characterized in that: two positioning columns (81) on two sides of the plate body (4) are respectively a cylinder and a prism.
7. The preservation container for freezing and storing cell liquid nitrogen according to claim 1, characterized in that: the diameter of the ring groove (41) is consistent with the minor axis of the elliptical plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122396347.0U CN215958000U (en) | 2021-09-30 | 2021-09-30 | Cell liquid nitrogen freezes deposits uses storage container |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122396347.0U CN215958000U (en) | 2021-09-30 | 2021-09-30 | Cell liquid nitrogen freezes deposits uses storage container |
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| CN215958000U true CN215958000U (en) | 2022-03-08 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115428787A (en) * | 2022-09-29 | 2022-12-06 | 邵阳学院 | Cryopreservation device for researching cell culture based on molecular biological DNA |
| CN116806808A (en) * | 2023-03-17 | 2023-09-29 | 王全军 | CAR-T cell cryopreservation box and cryopreservation method |
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2021
- 2021-09-30 CN CN202122396347.0U patent/CN215958000U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115428787A (en) * | 2022-09-29 | 2022-12-06 | 邵阳学院 | Cryopreservation device for researching cell culture based on molecular biological DNA |
| CN115428787B (en) * | 2022-09-29 | 2023-10-13 | 邵阳学院 | Freezing device for researching cell culture based on molecular biological DNA |
| CN116806808A (en) * | 2023-03-17 | 2023-09-29 | 王全军 | CAR-T cell cryopreservation box and cryopreservation method |
| CN116806808B (en) * | 2023-03-17 | 2024-02-13 | 淮安市第二人民医院 | CAR-T cell cryopreservation box and cryopreservation method |
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