JP2020513823A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2020513823A5 JP2020513823A5 JP2019551936A JP2019551936A JP2020513823A5 JP 2020513823 A5 JP2020513823 A5 JP 2020513823A5 JP 2019551936 A JP2019551936 A JP 2019551936A JP 2019551936 A JP2019551936 A JP 2019551936A JP 2020513823 A5 JP2020513823 A5 JP 2020513823A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- marker
- population
- nfia
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims description 104
- 238000000034 method Methods 0.000 claims description 99
- 210000000130 stem cell Anatomy 0.000 claims description 54
- 101001111328 Homo sapiens Nuclear factor 1 A-type Proteins 0.000 claims description 49
- 102100024006 Nuclear factor 1 A-type Human genes 0.000 claims description 45
- 230000011664 signaling Effects 0.000 claims description 45
- 230000002518 glial effect Effects 0.000 claims description 36
- 239000012190 activator Substances 0.000 claims description 33
- 239000002771 cell marker Substances 0.000 claims description 32
- 238000000338 in vitro Methods 0.000 claims description 31
- 239000000203 mixture Substances 0.000 claims description 31
- 239000003112 inhibitor Substances 0.000 claims description 30
- 210000001178 neural stem cell Anatomy 0.000 claims description 23
- 210000001130 astrocyte Anatomy 0.000 claims description 20
- 239000003550 marker Substances 0.000 claims description 20
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 14
- 230000010190 G1 phase Effects 0.000 claims description 13
- 102000007350 Bone Morphogenetic Proteins Human genes 0.000 claims description 10
- 108010007726 Bone Morphogenetic Proteins Proteins 0.000 claims description 10
- 102000003693 Hedgehog Proteins Human genes 0.000 claims description 10
- 108090000031 Hedgehog Proteins Proteins 0.000 claims description 10
- 101000687905 Homo sapiens Transcription factor SOX-2 Proteins 0.000 claims description 10
- 102100024270 Transcription factor SOX-2 Human genes 0.000 claims description 10
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims description 10
- 229940112869 bone morphogenetic protein Drugs 0.000 claims description 10
- 230000014509 gene expression Effects 0.000 claims description 10
- 229930002330 retinoic acid Natural products 0.000 claims description 10
- 229960001727 tretinoin Drugs 0.000 claims description 10
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims description 9
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims description 9
- 108010036280 Aquaporin 4 Proteins 0.000 claims description 8
- 102000012002 Aquaporin 4 Human genes 0.000 claims description 8
- 102100032912 CD44 antigen Human genes 0.000 claims description 8
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 8
- 101000652332 Homo sapiens Transcription factor SOX-1 Proteins 0.000 claims description 7
- 101100377226 Homo sapiens ZBTB16 gene Proteins 0.000 claims description 7
- 108700003766 Promyelocytic Leukemia Zinc Finger Proteins 0.000 claims description 7
- 102100030248 Transcription factor SOX-1 Human genes 0.000 claims description 7
- 102100040314 Zinc finger and BTB domain-containing protein 16 Human genes 0.000 claims description 7
- 208000015122 neurodegenerative disease Diseases 0.000 claims description 7
- 102000008730 Nestin Human genes 0.000 claims description 6
- 108010088225 Nestin Proteins 0.000 claims description 6
- 108010032788 PAX6 Transcription Factor Proteins 0.000 claims description 6
- 102100037506 Paired box protein Pax-6 Human genes 0.000 claims description 6
- 210000005055 nestin Anatomy 0.000 claims description 6
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 claims description 5
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 claims description 5
- 230000022131 cell cycle Effects 0.000 claims description 5
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 claims description 5
- 230000001537 neural effect Effects 0.000 claims description 5
- 210000002569 neuron Anatomy 0.000 claims description 5
- 210000000278 spinal cord Anatomy 0.000 claims description 5
- 102100031051 Cysteine and glycine-rich protein 1 Human genes 0.000 claims description 4
- 108010000720 Excitatory Amino Acid Transporter 2 Proteins 0.000 claims description 4
- 102100031562 Excitatory amino acid transporter 2 Human genes 0.000 claims description 4
- 101000922020 Homo sapiens Cysteine and glycine-rich protein 1 Proteins 0.000 claims description 4
- 101000979001 Homo sapiens Methionine aminopeptidase 2 Proteins 0.000 claims description 4
- 101000969087 Homo sapiens Microtubule-associated protein 2 Proteins 0.000 claims description 4
- 101000572986 Homo sapiens POU domain, class 3, transcription factor 2 Proteins 0.000 claims description 4
- 101000711846 Homo sapiens Transcription factor SOX-9 Proteins 0.000 claims description 4
- 102100023174 Methionine aminopeptidase 2 Human genes 0.000 claims description 4
- 102100026459 POU domain, class 3, transcription factor 2 Human genes 0.000 claims description 4
- 101150026440 S100b gene Proteins 0.000 claims description 4
- 102100034204 Transcription factor SOX-9 Human genes 0.000 claims description 4
- 206010002026 amyotrophic lateral sclerosis Diseases 0.000 claims description 4
- 230000001054 cortical effect Effects 0.000 claims description 4
- 230000004069 differentiation Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 4
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 4
- -1 small molecule compound Chemical class 0.000 claims description 4
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims description 4
- 238000011144 upstream manufacturing Methods 0.000 claims description 4
- 230000006378 damage Effects 0.000 claims description 3
- 239000002243 precursor Substances 0.000 claims description 3
- CDOVNWNANFFLFJ-UHFFFAOYSA-N 4-[6-[4-(1-piperazinyl)phenyl]-3-pyrazolo[1,5-a]pyrimidinyl]quinoline Chemical compound C1CNCCN1C1=CC=C(C2=CN3N=CC(=C3N=C2)C=2C3=CC=CC=C3N=CC=2)C=C1 CDOVNWNANFFLFJ-UHFFFAOYSA-N 0.000 claims description 2
- 208000024827 Alzheimer disease Diseases 0.000 claims description 2
- 241000283690 Bos taurus Species 0.000 claims description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
- 102000038594 Cdh1/Fizzy-related Human genes 0.000 claims description 2
- 108091007854 Cdh1/Fizzy-related Proteins 0.000 claims description 2
- 102100028404 Homeobox protein Hox-B4 Human genes 0.000 claims description 2
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims description 2
- 101000839788 Homo sapiens Homeobox protein Hox-B4 Proteins 0.000 claims description 2
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims description 2
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims description 2
- 101001053263 Homo sapiens Insulin gene enhancer protein ISL-1 Proteins 0.000 claims description 2
- 101000984042 Homo sapiens Protein lin-28 homolog A Proteins 0.000 claims description 2
- 102100024392 Insulin gene enhancer protein ISL-1 Human genes 0.000 claims description 2
- 102000002356 Nectin Human genes 0.000 claims description 2
- 108060005251 Nectin Proteins 0.000 claims description 2
- 208000018737 Parkinson disease Diseases 0.000 claims description 2
- 102100025460 Protein lin-28 homolog A Human genes 0.000 claims description 2
- 208000006289 Rett Syndrome Diseases 0.000 claims description 2
- 230000024245 cell differentiation Effects 0.000 claims description 2
- 239000012091 fetal bovine serum Substances 0.000 claims description 2
- 230000001605 fetal effect Effects 0.000 claims description 2
- 231100000878 neurological injury Toxicity 0.000 claims description 2
- 230000002018 overexpression Effects 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims description 2
- 210000002966 serum Anatomy 0.000 claims description 2
- 210000004500 stellate cell Anatomy 0.000 claims description 2
- 108010059616 Activins Proteins 0.000 claims 1
- 101710088083 Glomulin Proteins 0.000 claims 1
- 102100026818 Inhibin beta E chain Human genes 0.000 claims 1
- 102100023832 Prolyl endopeptidase FAP Human genes 0.000 claims 1
- 102000009618 Transforming Growth Factors Human genes 0.000 claims 1
- 108010009583 Transforming Growth Factors Proteins 0.000 claims 1
- 239000000488 activin Substances 0.000 claims 1
- 230000001143 conditioned effect Effects 0.000 claims 1
- 108091006086 inhibitor proteins Proteins 0.000 claims 1
- 102000004058 Leukemia inhibitory factor Human genes 0.000 description 4
- 108090000581 Leukemia inhibitory factor Proteins 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 3
- 208000028389 Nerve injury Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008764 nerve damage Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022160941A JP2022180634A (ja) | 2017-03-21 | 2022-10-05 | 幹細胞由来星状細胞、作製方法および使用方法 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762474429P | 2017-03-21 | 2017-03-21 | |
| US201762474596P | 2017-03-21 | 2017-03-21 | |
| US62/474,596 | 2017-03-21 | ||
| US62/474,429 | 2017-03-21 | ||
| PCT/US2018/023551 WO2018175574A1 (en) | 2017-03-21 | 2018-03-21 | Stem cell-derived astrocytes, methods of making and methods of use |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022160941A Division JP2022180634A (ja) | 2017-03-21 | 2022-10-05 | 幹細胞由来星状細胞、作製方法および使用方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020513823A JP2020513823A (ja) | 2020-05-21 |
| JP2020513823A5 true JP2020513823A5 (https=) | 2021-04-30 |
| JP7471084B2 JP7471084B2 (ja) | 2024-04-19 |
Family
ID=63586143
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019551936A Active JP7471084B2 (ja) | 2017-03-21 | 2018-03-21 | 幹細胞由来星状細胞、作製方法および使用方法 |
| JP2022160941A Withdrawn JP2022180634A (ja) | 2017-03-21 | 2022-10-05 | 幹細胞由来星状細胞、作製方法および使用方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022160941A Withdrawn JP2022180634A (ja) | 2017-03-21 | 2022-10-05 | 幹細胞由来星状細胞、作製方法および使用方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20200024574A1 (https=) |
| EP (1) | EP3600360A4 (https=) |
| JP (2) | JP7471084B2 (https=) |
| AU (2) | AU2018239426A1 (https=) |
| CA (1) | CA3057104A1 (https=) |
| WO (1) | WO2018175574A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3787613B1 (en) | 2018-04-30 | 2026-03-11 | Cedars-Sinai Medical Center | Pkc activation for use in treating early onset sporadic parkinson's disease |
| AU2020370288B2 (en) * | 2019-10-22 | 2026-02-12 | Cedars-Sinai Medical Center | Cortical neural progenitor cells from iPSCs |
| EP4206320A4 (en) * | 2020-08-21 | 2025-02-12 | JSR Corporation | METHOD FOR CULTIVATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS, CULTURE OF HUMAN INDUCED PLURIPOTENT STEM CELLS AND METHOD FOR THE PRODUCTION OF CEREBRAL ORGANOIDS |
| CN116406303B (zh) * | 2020-09-04 | 2025-07-29 | 孔二艳 | 将gfap氨基酸位点作为神经退行性疾病治疗靶标的应用 |
| EP4212615A4 (en) * | 2020-09-10 | 2024-11-27 | JSR Corporation | METHOD FOR THE PRODUCTION OF ASTROCYTE-LIKE CELLS |
| CN111961650B (zh) * | 2020-10-20 | 2023-06-13 | 士泽生物医药（苏州）有限公司 | 一种体外获得神经胶质细胞的方法及其应用 |
| WO2022251254A1 (en) * | 2021-05-24 | 2022-12-01 | The Johns Hopkins University | Pharmacological intervention of the arachidonic acid pathway to cure amyotrophic lateral sclerosis |
| CN116478923B (zh) * | 2022-04-26 | 2024-01-02 | 浙江霍德生物工程有限公司 | 一种星形胶质细胞的制备方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6001654A (en) * | 1994-01-28 | 1999-12-14 | California Institute Of Technology | Methods for differentiating neural stem cells to neurons or smooth muscle cells using TGT-β super family growth factors |
| JP5943533B2 (ja) * | 2000-05-17 | 2016-07-06 | アステリアス バイオセラピューティクス インコーポレイテッド | 神経前駆細胞の集団 |
| JP2009513269A (ja) * | 2005-10-27 | 2009-04-02 | アルフォース， ジャン−エリック ダブリュー． | 無細胞血液産物をインキュベートすることによって生成される無細胞、生体吸収性の組織再生マトリックス |
| US20120207744A1 (en) * | 2009-03-19 | 2012-08-16 | Mendlein John D | Reprogramming compositions and methods of using the same |
| US9453198B2 (en) * | 2010-05-25 | 2016-09-27 | Memorial Sloan Kettering Cancer Center | Method of nociceptor differentiation of human embryonic stem cells and uses thereof |
| PT2773748T (pt) * | 2011-11-04 | 2020-03-26 | Memorial Sloan Kettering Cancer Center | Neurónios dopaminérgicos (da) do mesencéfalo para enxerto |
| CN105960453A (zh) * | 2013-10-01 | 2016-09-21 | 卡迪马干细胞有限公司 | 从人多能干细胞定向分化星形胶质细胞用于肌萎缩侧索硬化(als)的治疗和药物筛选 |
| WO2016103269A1 (en) * | 2014-12-23 | 2016-06-30 | Ramot At Tel-Aviv University Ltd. | Populations of neural progenitor cells and methods of producing and using same |
| WO2017057523A1 (ja) * | 2015-09-29 | 2017-04-06 | 学校法人慶應義塾 | アストロサイト様細胞及びその調製方法 |
-
2018
- 2018-03-21 JP JP2019551936A patent/JP7471084B2/ja active Active
- 2018-03-21 WO PCT/US2018/023551 patent/WO2018175574A1/en not_active Ceased
- 2018-03-21 CA CA3057104A patent/CA3057104A1/en active Pending
- 2018-03-21 AU AU2018239426A patent/AU2018239426A1/en not_active Abandoned
- 2018-03-21 EP EP18770878.9A patent/EP3600360A4/en active Pending
-
2019
- 2019-09-20 US US16/576,956 patent/US20200024574A1/en active Pending
-
2022
- 2022-10-05 JP JP2022160941A patent/JP2022180634A/ja not_active Withdrawn
-
2024
- 2024-05-22 AU AU2024203389A patent/AU2024203389A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2020513823A5 (https=) | ||
| JP7245752B2 (ja) | 多能性幹細胞から生成された光受容体および光受容体前駆体 | |
| JP6262205B2 (ja) | ヒト多能性幹細胞からの神経幹細胞およびドーパミン作動性ニューロンの誘導方法 | |
| US10449221B2 (en) | Differentiation of stem cells into thyroid tissue | |
| EP2486127B1 (en) | Compositions for inducing differentiation into retinal cells from retinal progenitor cells or inducing proliferation of retinal cells comprising wnt signaling pathway activators | |
| CN107810268B (zh) | 通过直接重编程由引入Oct4的人体细胞诱导少突胶质细胞前体细胞的方法 | |
| US11857697B2 (en) | Compositions and methods for obtaining 3-dimensional lung-like epithelium and related uses thereof | |
| JP6316938B2 (ja) | Ｈｍｇａ２を用いて非神経細胞からリプログラミングされた誘導神経幹細胞を調製する方法 | |
| US20170260501A1 (en) | Derivation of neural crest stem cells and uses thereof | |
| CN107075481B (zh) | 制备腺垂体或其前体组织的方法 | |
| JP7598314B2 (ja) | 合成メッセンジャーｒｎａを用いて尿細胞を神経幹細胞へ直接逆分化する方法 | |
| Lim et al. | Generation of dopamine neurons from rodent fibroblasts through the expandable neural precursor cell stage | |
| KR101903458B1 (ko) | 슈반 세포 전구체 (Schwann cell precursor) 및 이로부터 분화된 슈반 세포 (Schwann cell)의 제조 방법 | |
| JP2018076385A (ja) | 神経前駆細胞を用いた細胞治療における移植補助剤 | |
| Li et al. | Multipotent neural crest stem cell‐like cells from rat vibrissa dermal papilla induce neuronal differentiation of PC12 cells | |
| Insua et al. | Trophic factors and neuronal interactions regulate the cell cycle and Pax6 expression in Müller stem cells | |
| KR20130011023A (ko) | 전능성 줄기세포로부터 희소돌기아교전구세포의 제조 방법 | |
| Rzhanova et al. | Reprogramming of differentiated mammalian and human retinal pigment epithelium: Current achievements and prospects | |
| Goureau et al. | Stem cells in the retina: regeneration and cell therapy | |
| Mitashov | Multipotent and stem cells in the developing, definitive, and regenerating vertebrate eye | |
| Warrington | Defining a small molecules differentiation cocktail for generation of dopaminergic neurons from Wharton’s jelly mesenchymal stem cells | |
| Lin | Investigating the role of Neuronatin in neural development | |
| Jaeger | Midbrain dopaminergic neuron fate specification of pluripotent stem cells | |
| Kaltschmidt et al. | NEURAL CREST STEM CELLS FROM THE HEAD REGION |