JP2020510406A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2020510406A5 JP2020510406A5 JP2019524990A JP2019524990A JP2020510406A5 JP 2020510406 A5 JP2020510406 A5 JP 2020510406A5 JP 2019524990 A JP2019524990 A JP 2019524990A JP 2019524990 A JP2019524990 A JP 2019524990A JP 2020510406 A5 JP2020510406 A5 JP 2020510406A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- sensor
- pseudomonas spp
- target sequence
- sensor according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091005771 Peptidases Proteins 0.000 claims 11
- 239000004365 Protease Substances 0.000 claims 11
- 241000589516 Pseudomonas Species 0.000 claims 11
- 102000033147 ERVK-25 Human genes 0.000 claims 7
- 230000001809 detectable Effects 0.000 claims 5
- 102000035443 Peptidases Human genes 0.000 claims 4
- 238000003776 cleavage reaction Methods 0.000 claims 3
- 235000013365 dairy product Nutrition 0.000 claims 3
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 239000005090 green fluorescent protein Substances 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 239000005089 Luciferase Substances 0.000 claims 1
- 108060001084 Luciferase family Proteins 0.000 claims 1
- 241000545067 Venus Species 0.000 claims 1
- 238000002835 absorbance Methods 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 102000004965 antibodies Human genes 0.000 claims 1
- 108090001123 antibodies Proteins 0.000 claims 1
- 201000009910 diseases by infectious agent Diseases 0.000 claims 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 1
- 239000002105 nanoparticle Substances 0.000 claims 1
- 239000002096 quantum dot Substances 0.000 claims 1
- 238000002165 resonance energy transfer Methods 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 claims 1
- -1 viologens Substances 0.000 claims 1
Claims (13)
- Ｐｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼセンサー分子であって、ＫＱ、ＳＦ、ＦＭ、ＫＫ、ＫＮ、ＮＱ、ＮＴ、ＥＩ、ＱＱ、及びそれらの組み合わせからなる群より選択される少なくとも１つのＰｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼ切断部位、ならびに以下の特徴：
（ｉ）前記標的配列の長さが、約５０アミノ酸未満である、
（ｉｉ）検出可能な標識、及び／または
（ｉｉｉ）前記プロテアーゼ切断部位のうちの２つ以上、
のうちの１つ以上を有する標的配列を含み、
前記Ｐｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼによる標的配列の切断が、検出可能な変化を生じる、前記Ｐｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼセンサー分子。 - 前記標的配列が、ＳＦＭ（配列番号３７）、ＳＫＭＸＸＰＰ（式中、Ｘは、任意のアミノ酸（配列番号４０）である）、及び／またはＫＫＮＱ（配列番号２４）を少なくとも含む、請求項１に記載のセンサー。
- 前記標的配列が、
ＳＦＭＫＫＮＱＮＴＥＩ（配列番号４５）、
ＳＦＭＮＱＮＴＥＩ（配列番号５７）、
ＬＳＦＭＡＩＰ（配列番号７０）、
ＬＱＧＳＦＭＫＫＮＱＮＴＥＩＧＳＦＥ（配列番号７２）、
ＬＱＧＳＦＭＮＱＮＴＥＩＧＳＦＥ（配列番号７３）、
ＬＱＧＳＬＳＦＭＡＩＰＧＳＦＥ（配列番号７４）、及び
ＬＱＧＳＫＱＫＱＫＱＫＱＧＳＦＥ（配列番号１１２）、
から選択されるアミノ酸配列を含む、請求項１に記載のセンサー。 - 前記検出可能な変化が、共鳴エネルギー移動、音響、電気化学ポテンシャル、蛍光、化学発光、燐光、吸光度、抗体結合、ＢＲＥＴ比、ＦＲＥＴ効率、または質量の変化である、請求項１〜３のいずれか１項に記載のセンサー。
- 前記検出可能な標識が、発色団、ナノ粒子、量子ドット、ビオロゲン、及びそれらの組み合わせからなる群より選択される、請求項１〜４のいずれか１項に記載のセンサー。
- 前記検出可能な標識が、
ｉ）前記標的配列に共有結合されている化学発光ドナードメイン及びアクセプタードメイン、または
ｉｉ）蛍光団ドメイン及びアクセプタードメイン、
含む、請求項１〜５のいずれか１項に記載のセンサー。 - 前記アクセプタードメインが、緑色蛍光タンパク質（ＧＦＰ）、Ｖｅｎｕｓ、ｍＯｒａｎｇｅ、またはそれらのうちのいずれか１つの生物学的に活性な多様体もしくはフラグメントであり、
前記化学発光ドナードメインが、ルシフェラーゼまたは生物学的に活性な多様体もしくはフラグメントであり、
前記標的配列が、ＳＦＭＫＫＮＱＮＴＥＩ（配列番号４５）、ＳＦＭＮＱＮＴＥＩ（配列番号５７）、またはＬＳＦＭＡＩＰ（配列番号７０）を含む、請求項６に記載のセンサー。 - ｉ）Ｐｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼの場合、０．１〜１０ｎＭ、もしくは０．１〜１００ｎＭのＥＣ50を有し、及び／または
ｉｉ）ウシプラスミンと比較して、Ｐｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼへの感度が、少なくとも２０倍、または少なくとも１００倍高い、
請求項１〜７のいずれか１項に記載のセンサー。 - 請求項１〜８のいずれか１項に記載のセンサーもしくはその標的配列をコードする単離核酸、または請求項１〜８のいずれか１項に記載のセンサーもしくはその標的配列をコードする核酸を含むベクター。
- ｉ）試料中のＰｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼを検出するための、
ｉｉ）乳製品の腐敗を検出するための、または
ｉｉｉ）Ｐｓｅｕｄｏｍｏｎａｓ ｓｐｐ．感染を検出するための、
請求項１〜８のいずれか１項に記載のセンサーの使用。 - 試料中のＰｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼを検出する方法であって、前記方法が、
ｉ）請求項１〜８のいずれか１項に記載のセンサーと、試料を接触させること、及び
ｉｉ）前記センサーの変化を検出すること、
を含み、前記変化が、前記試料中のＰｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼの存在に対応する、前記方法。 - 乳製品の腐敗を検出する方法であって、前記方法が、
ｉ）請求項１〜８のいずれか１項に記載のセンサーと、試料を接触させること、及び
ｉｉ）前記センサーの変化を検出すること、
を含み、前記変化が、前記乳製品が腐敗しているか、腐敗し始めているか、または腐敗する可能性があることを示す、前記方法。 - ｉ）少なくとも１ｐＭ、もしくは少なくとも１０ｐＭ、もしくは少なくとも１００ｐｍのＰｓｅｕｄｏｍｏｎａｓ ｓｐｐ．プロテアーゼを検出し、及び／または
ｉｉ）１時間未満、３０分未満、または１５分未満で実施される、
請求項１１または請求項１２に記載の方法。
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2016904639A AU2016904639A0 (en) | 2016-11-14 | Protease sensor molecules | |
| AU2016904639 | 2016-11-14 | ||
| AU2017900161 | 2017-01-19 | ||
| AU2017900161A AU2017900161A0 (en) | 2017-01-19 | Protease sensor molecules | |
| PCT/AU2017/051237 WO2018085895A1 (en) | 2016-11-14 | 2017-11-13 | Protease sensor molecules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020510406A JP2020510406A (ja) | 2020-04-09 |
| JP2020510406A5 true JP2020510406A5 (ja) | 2020-12-24 |
Family
ID=62108989
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019524990A Pending JP2020510406A (ja) | 2016-11-14 | 2017-11-13 | プロテアーゼセンサー分子 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US11952612B2 (ja) |
| EP (1) | EP3538665B1 (ja) |
| JP (1) | JP2020510406A (ja) |
| CN (1) | CN110494569A (ja) |
| AU (1) | AU2017358067B2 (ja) |
| IL (1) | IL266577A (ja) |
| PL (1) | PL3538665T3 (ja) |
| WO (1) | WO2018085895A1 (ja) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013204332B2 (en) | 2012-04-16 | 2015-07-16 | Commonwealth Scientific And Industrial Research Organisation | Methods and systems for detecting an analyte or classifying a sample |
| CN109187511B (zh) * | 2018-09-10 | 2021-04-20 | 浙江理工大学 | 一种基于电化学发光法检测古代丝织品的方法 |
| WO2020214573A1 (en) * | 2019-04-15 | 2020-10-22 | The Government Of The United States Of America, As Represented By The Secretary Of The Navy | Enzyme transducers and sensors based on dna loops |
| CN113049555B (zh) * | 2021-03-12 | 2022-09-30 | 师大瑞利光电科技(清远)有限公司 | 基于同一体系细胞样本测量激发发射光谱线性分离定量fret系统校正因子的方法及应用 |
| NL2027785B1 (en) | 2021-03-19 | 2022-09-29 | Original G B V | Method for the in vitro diagnosis of infection |
| CN113444822A (zh) * | 2021-07-09 | 2021-09-28 | 石家庄君乐宝乳业有限公司 | 检测乳制品中荧光假单胞菌和碱性蛋白酶的引物及相应的检测方法 |
| CN114295587B (zh) * | 2021-12-29 | 2023-07-21 | 上海大学 | 一种基于二维金属有机框架的spr传感器及其制备和应用 |
Family Cites Families (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2191110B (en) | 1986-06-06 | 1989-12-06 | Plessey Co Plc | Chromatographic separation device |
| US4908112A (en) | 1988-06-16 | 1990-03-13 | E. I. Du Pont De Nemours & Co. | Silicon semiconductor wafer for analyzing micronic biological samples |
| US5196524A (en) | 1989-01-06 | 1993-03-23 | Eli Lilly And Company | Fusion reporter gene for bacterial luciferase |
| US5126022A (en) | 1990-02-28 | 1992-06-30 | Soane Tecnologies, Inc. | Method and device for moving molecules by the application of a plurality of electrical fields |
| US5750015A (en) | 1990-02-28 | 1998-05-12 | Soane Biosciences | Method and device for moving molecules by the application of a plurality of electrical fields |
| US5219737A (en) | 1990-03-27 | 1993-06-15 | Kikkoman Corporation | Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase |
| US5229285A (en) | 1991-06-27 | 1993-07-20 | Kikkoman Corporation | Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly |
| US5580523A (en) | 1994-04-01 | 1996-12-03 | Bard; Allen J. | Integrated chemical synthesizers |
| JP3466765B2 (ja) | 1994-07-27 | 2003-11-17 | キッコーマン株式会社 | ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法 |
| US6001229A (en) | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| US5571410A (en) | 1994-10-19 | 1996-11-05 | Hewlett Packard Company | Fully integrated miniaturized planar liquid sample handling and analysis device |
| US5670356A (en) | 1994-12-12 | 1997-09-23 | Promega Corporation | Modified luciferase |
| US6228604B1 (en) | 1996-04-10 | 2001-05-08 | Loma Linda University | Secreted renilla luciferase |
| US5885470A (en) | 1997-04-14 | 1999-03-23 | Caliper Technologies Corporation | Controlled fluid transport in microfabricated polymeric substrates |
| DE69938293T2 (de) | 1998-03-27 | 2009-03-12 | Bruce J. Beverly Hills Bryan | Luciferase, gfp fluoreszenzproteine, kodierende nukleinsaüre und ihre verwendung in der diagnose |
| ATE435654T1 (de) | 1998-07-17 | 2009-07-15 | Univ Maryland | Manipulierte proteine zum analyten nachweis |
| GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| CA2431859C (en) * | 1998-11-02 | 2006-02-21 | Genzyme Transgenics Corp. | Transgenic and cloned mammals |
| AU779988B2 (en) | 1999-06-28 | 2005-02-24 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| WO2001046691A1 (en) | 1999-12-22 | 2001-06-28 | Biosignal Packard Inc. | A bioluminescence resonance energy transfer (bret) system with broad spectral resolution between donor and acceptor emission wavelengths and its use |
| AU2137101A (en) | 1999-12-22 | 2001-07-03 | Biosignal Packard Inc. | A bioluminescence resonance energy transfer (bret) fusion molecule and method ofuse |
| EP1356081B1 (en) | 2000-05-03 | 2006-08-23 | Expressive Constructs, Inc. | Method for detecting listeria monocytogenes |
| WO2002048195A2 (en) | 2000-10-23 | 2002-06-20 | Engeneos, Inc. | Engineered stimulus-responsive switches |
| US6949377B2 (en) | 2001-03-05 | 2005-09-27 | Ho Winston Z | Chemiluminescence-based microfluidic biochip |
| WO2003015923A1 (en) | 2001-08-20 | 2003-02-27 | Biomicro Systems, Inc. | Fluid mixing in low aspect ratio chambers |
| US7338760B2 (en) | 2001-10-26 | 2008-03-04 | Ntu Ventures Private Limited | Sample preparation integrated chip |
| CA2475516A1 (en) | 2002-03-05 | 2003-09-18 | Aclara Biosciences, Inc. | Multiplex analysis using membrane-bound sensitizers |
| US6942987B2 (en) | 2002-05-15 | 2005-09-13 | Pharmacopeia Drug Discovery, Inc. | Methods for measuring kinase activity |
| ES2237643T3 (es) | 2002-10-21 | 2005-08-01 | Axxam S.R.L. | Fotoproteina con bioluminiscencia mejorada. |
| CN1802432A (zh) * | 2003-04-23 | 2006-07-12 | 赫希玛有限公司 | 昆虫胰凝乳蛋白酶及其抑制剂 |
| WO2006098772A2 (en) | 2004-10-13 | 2006-09-21 | U.S. Genomics, Inc. | Systems and methods for measurement optimization |
| WO2006105616A1 (en) | 2005-04-08 | 2006-10-12 | Commonwealth Scientific And Industrial Research Organisation | Method for microfluidic mixing and mixing device |
| JP2009505642A (ja) | 2005-08-19 | 2009-02-12 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション | アラクノカンパのルシフェラーゼ |
| EP1938101A2 (en) | 2005-09-13 | 2008-07-02 | Fluidigm Corporation | Microfluidic assay devices and methods |
| AU2006315332A1 (en) | 2005-11-16 | 2007-05-24 | Carnegie Institution Of Washington | Multimeric biosensors and methods of using the same |
| WO2007092909A2 (en) * | 2006-02-07 | 2007-08-16 | Expressive Constructs, Inc. | Molecular interaction sensors |
| DE102007002580A1 (de) * | 2007-01-11 | 2008-07-17 | C-Lecta Gmbh | Proteolytische Abspaltung von Protein-Tags in der rekombinanten Protein-Herstellung |
| US9034402B2 (en) | 2007-04-16 | 2015-05-19 | Solae, Llc | Protein hydrolysate compositions having improved sensory characteristics and physical properties |
| EP2149050A2 (en) | 2007-04-27 | 2010-02-03 | The Regents Of The University Of California | Device and methods for detection of airborne agents |
| WO2009018467A2 (en) | 2007-07-31 | 2009-02-05 | Massachusetts Institute Of Technology | Bio-sensing nanodevice |
| CN202122963U (zh) | 2007-10-02 | 2012-01-25 | 阿莱瑞士股份有限公司 | 分析装置 |
| WO2009052504A1 (en) | 2007-10-18 | 2009-04-23 | University Of Medicine & Dentistry Of New Jersey | Systems for and methods of detecting mastitis |
| JP5737673B2 (ja) | 2008-11-10 | 2015-06-17 | 国立大学法人名古屋大学 | アレルゲンのエピトープ又はその候補の検出方法及びその利用 |
| US8268977B2 (en) | 2008-11-20 | 2012-09-18 | The Board Of Trustees Of The Leland Stanford Junior University | Strongly quenching oligomeric excimer/quencher pairs for detection schemes |
| US8647887B2 (en) | 2009-01-29 | 2014-02-11 | Commonwealth Scientific And Industrial Research Organisation | Measuring G protein coupled receptor activation |
| EP2221606A3 (en) | 2009-02-11 | 2012-06-06 | Samsung Electronics Co., Ltd. | Integrated bio-chip and method of fabricating the integrated bio-chip |
| JP2011038922A (ja) | 2009-08-12 | 2011-02-24 | Sony Corp | 光検出用チップおよび該光検出用チップを用いた光検出装置 |
| US8524457B2 (en) | 2009-09-22 | 2013-09-03 | William Patterson | Method for the selection of specific affinity binders by homogeneous noncompetitive assay |
| WO2011091037A2 (en) | 2010-01-19 | 2011-07-28 | President And Fellows Of Harvard College | Rapid pathogen diagnostic device and method |
| US8759070B2 (en) | 2010-09-10 | 2014-06-24 | University Of Delaware | Recombinant clostridia that fix CO2 and CO and uses thereof |
| US8828678B2 (en) | 2010-11-16 | 2014-09-09 | Enzo Life Sciences, Inc. | Self-immolative probes for enzyme activity detection |
| AU2013204332B2 (en) * | 2012-04-16 | 2015-07-16 | Commonwealth Scientific And Industrial Research Organisation | Methods and systems for detecting an analyte or classifying a sample |
| IN2015DN00829A (ja) | 2012-07-02 | 2015-06-12 | Hydration Systems Llc | |
| US9193990B2 (en) | 2013-03-15 | 2015-11-24 | Richard B. Thompson | Bioluminescent metal ion assay |
| JP6040846B2 (ja) | 2013-04-08 | 2016-12-07 | Jnc株式会社 | セレンテラジン類縁体 |
| WO2014207515A1 (en) | 2013-06-25 | 2014-12-31 | Tubitak (Turkiye Bilimsel Ve Teknolojik Arastirma Kurumu) | Fluorescence method for detecting nuclease activity |
| CA2918426C (en) | 2013-07-18 | 2021-10-19 | Ecole Polytechnique Federale De Lausanne (Epfl) | Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample |
| CN106662578B (zh) | 2014-04-03 | 2021-11-12 | 艾勒詹尼斯有限责任公司 | 用于检测食物变态反应的肽、试剂和方法 |
| US9610358B2 (en) | 2014-04-17 | 2017-04-04 | Marker Gene Technologies, Inc | Targeted pharmacological chaperones |
| JP6704404B2 (ja) | 2015-02-16 | 2020-06-03 | マックス−プランク−ゲゼルシャフト ツール フォーデルング デル ヴィッセンシャフテン エー．ヴェー． | ニコチンアミドアデニンジヌクレオチドを検出するためのセンサー、方法及びキット |
| CA3005883A1 (en) | 2015-11-20 | 2017-05-26 | Duke University | Ratiometric biosensors and non-geometrically modulated fret |
| CA3071994A1 (en) | 2017-08-08 | 2019-02-14 | Commonwealth Scientific And Industrial Research Organisation | Carbohydrate sensors |
| US20210018497A1 (en) | 2017-08-24 | 2021-01-21 | Commonwealth Scientific And Industrial Research Organisation | Bret sensor molecules for detecting hydrolases |
-
2017
- 2017-11-13 AU AU2017358067A patent/AU2017358067B2/en active Active
- 2017-11-13 WO PCT/AU2017/051237 patent/WO2018085895A1/en unknown
- 2017-11-13 EP EP17869718.1A patent/EP3538665B1/en active Active
- 2017-11-13 PL PL17869718.1T patent/PL3538665T3/pl unknown
- 2017-11-13 JP JP2019524990A patent/JP2020510406A/ja active Pending
- 2017-11-13 US US16/349,237 patent/US11952612B2/en active Active
- 2017-11-13 CN CN201780082959.6A patent/CN110494569A/zh active Pending
-
2019
- 2019-05-12 IL IL266577A patent/IL266577A/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2020510406A5 (ja) | ||
| Bhardwaj et al. | Fluorescent nanobiosensors for the targeted detection of foodborne bacteria | |
| Wu et al. | A sensitive lateral flow biosensor for Escherichia coli O157: H7 detection based on aptamer mediated strand displacement amplification | |
| Chen et al. | A novel fluorescence immunoassay for the sensitive detection of Escherichia coli O157: H7 in milk based on catalase-mediated fluorescence quenching of CdTe quantum dots | |
| Ikebukuro et al. | Novel electrochemical sensor system for protein using the aptamers in sandwich manner | |
| Van Dorst et al. | Recent advances in recognition elements of food and environmental biosensors: A review | |
| Abdelhamid et al. | Selective biosensing of Staphylococcus aureus using chitosan quantum dots | |
| US7642056B2 (en) | Method and kit for detecting a target protein using a DNA aptamer | |
| Anderson et al. | Single domain antibody–quantum dot conjugates for ricin detection by both fluoroimmunoassay and surface plasmon resonance | |
| Peltomaa et al. | Application of bacteriophages in sensor development | |
| Chen et al. | Bivalent aptasensor based on silver-enhanced fluorescence polarization for rapid detection of lactoferrin in milk | |
| Neethirajan et al. | Agro-defense: Biosensors for food from healthy crops and animals | |
| Kim et al. | Fluorescent ferritin nanoparticles and application to the aptamer sensor | |
| Jose et al. | Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor | |
| Liu et al. | Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157: H7 | |
| JP5463621B2 (ja) | 標的物質の定量方法 | |
| Gao et al. | Quantum‐dot‐encoded microbeads for multiplexed genetic detection of non‐amplified DNA samples | |
| Hayat et al. | Development of a novel label-free amperometric immunosensor for the detection of okadaic acid | |
| Singh et al. | An insight in bacteriophage based biosensors with focus on their detection methods and recent advancements | |
| CN108796041A (zh) | 一种基于生物发光共振能量转移的信号扩增系统及其检测方法 | |
| Islam et al. | Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria | |
| JP2020537489A5 (ja) | ||
| Chen et al. | Selection of specific DNA aptamers for hetero-sandwich-based colorimetric determination of Campylobacter jejuni in food | |
| Yao et al. | Enzyme-free and label-free detection of Staphylococcus aureus based on target-inhibited fluorescence signal recovery | |
| Oelschlaeger et al. | Fluorophor-linked immunosorbent assay: a time-and cost-saving method for the characterization of antibody fragments using a fusion protein of a single-chain antibody fragment and enhanced green fluorescent protein |