JP2020503846A - タグなしコード化化学ライブラリ - Google Patents

タグなしコード化化学ライブラリ Download PDF

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Publication number
JP2020503846A
JP2020503846A JP2019523655A JP2019523655A JP2020503846A JP 2020503846 A JP2020503846 A JP 2020503846A JP 2019523655 A JP2019523655 A JP 2019523655A JP 2019523655 A JP2019523655 A JP 2019523655A JP 2020503846 A JP2020503846 A JP 2020503846A
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library
tag
chemical structure
nucleic acid
microcompartment
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Pending
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JP2019523655A
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Japanese (ja)
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JP2020503846A5 (cg-RX-API-DMAC10.html
Inventor
デイヴィッド ヒュー ウィリアムズ，
デイヴィッド ヒュー ウィリアムズ，
イプシタ マンダール，
イプシタ マンダール，
スチュアート ロバート ウッド，
スチュアート ロバート ウッド，
ダニエル ブラットン，
ダニエル ブラットン，
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Astellas Engineered Small Molecules U.K. Ltd
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Nanna Therapeutics Ltd
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Priority claimed from GBGB1709718.9A external-priority patent/GB201709718D0/en
Application filed by Nanna Therapeutics Ltd filed Critical Nanna Therapeutics Ltd
Publication of JP2020503846A publication Critical patent/JP2020503846A/ja
Publication of JP2020503846A5 publication Critical patent/JP2020503846A5/ja
Priority to JP2022128528A priority Critical patent/JP2022180348A/ja
Pending legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/319—Photocleavage, photolysis, photoactivation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/146—Concentration of target or template
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Tape Cassette Changers (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2019523655A 2016-11-08 2017-11-08 タグなしコード化化学ライブラリ Pending JP2020503846A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022128528A JP2022180348A (ja) 2016-11-08 2022-08-10 タグなしコード化化学ライブラリ

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB201618820 2016-11-08
GB1618820.3 2016-11-08
GB1709718.9 2017-06-19
GBGB1709718.9A GB201709718D0 (en) 2017-06-19 2017-06-19 Tagless encoded chemical library
PCT/GB2017/053360 WO2018087539A1 (en) 2016-11-08 2017-11-08 Tagless encoded chemical library

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JP2022128528A Division JP2022180348A (ja) 2016-11-08 2022-08-10 タグなしコード化化学ライブラリ

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JP2020503846A true JP2020503846A (ja) 2020-02-06
JP2020503846A5 JP2020503846A5 (cg-RX-API-DMAC10.html) 2020-11-19

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EP (1) EP3538653B1 (cg-RX-API-DMAC10.html)
JP (2) JP2020503846A (cg-RX-API-DMAC10.html)
KR (1) KR102355956B1 (cg-RX-API-DMAC10.html)
CN (1) CN110249050B (cg-RX-API-DMAC10.html)
AU (1) AU2017357638B2 (cg-RX-API-DMAC10.html)
CA (1) CA3042974A1 (cg-RX-API-DMAC10.html)
IL (1) IL266318B2 (cg-RX-API-DMAC10.html)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023548062A (ja) * 2020-10-23 2023-11-15 イーティーエイチ チューリッヒ 自己精製された核酸コードライブラリー

Families Citing this family (13)

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US11084037B2 (en) * 2017-09-25 2021-08-10 Plexium, Inc. Oligonucleotide encoded chemical libraries
CN110658163A (zh) * 2018-06-29 2020-01-07 成都先导药物开发股份有限公司 一种合成dna编码化合物中的反应监测方法
GB201817321D0 (en) * 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
WO2020096015A1 (ja) * 2018-11-07 2020-05-14 国立大学法人 東京大学 １種以上の被検物質と共存した細胞のゲノム関連情報を検出する方法
EP3947659A4 (en) * 2019-04-02 2022-12-28 The Trustees of Columbia University in the City of New York SEQUENCING BY ENERGY TRANSFER DYE PAIR SYNTHESIS
CN113597471B (zh) 2019-04-16 2025-07-25 弗·哈夫曼-拉罗切有限公司 使用修饰珠的小分子筛选细胞分析
WO2020259689A1 (zh) * 2019-06-27 2020-12-30 成都先导药物开发股份有限公司 一种可识别混合物对复杂生物体系的鉴别方法
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
CN114761577B (zh) * 2020-01-10 2025-10-24 新克赛特株式会社 新型细胞表型筛选方法
CN111304753B (zh) * 2020-03-24 2020-12-01 深圳奇点药物科技有限公司 Dna编码分子库的筛选方法和试剂盒
US11628382B2 (en) * 2020-08-26 2023-04-18 Duke University Methods and apparatuses for purification of gel droplets supporting biological tissue
KR20230165231A (ko) * 2021-03-04 2023-12-05 오레곤 헬스 앤드 사이언스 유니버시티 분자 바코드 및 관련 방법 및 시스템
WO2023174845A1 (en) * 2022-03-13 2023-09-21 Eleven Therapeutics Ltd System and method for high throughput evaluation of chemical modifications of nucleic acid molecules

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Publication number Priority date Publication date Assignee Title
JP2023548062A (ja) * 2020-10-23 2023-11-15 イーティーエイチ チューリッヒ 自己精製された核酸コードライブラリー
CN117377759A (zh) * 2020-10-23 2024-01-09 约尔格·谢尔曼 自纯化的核酸编码的文库
JP7883239B2 (ja) 2020-10-23 2026-07-01 イェルク ショイエルマン 自己精製された核酸コードライブラリー

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EP3538653A1 (en) 2019-09-18
CA3042974A1 (en) 2018-05-17
US20190264199A1 (en) 2019-08-29
US20220073907A1 (en) 2022-03-10
EP3538653B1 (en) 2021-07-28
KR102355956B1 (ko) 2022-01-25
WO2018087539A1 (en) 2018-05-17
AU2017357638A1 (en) 2019-05-16
JP2022180348A (ja) 2022-12-06
IL266318B2 (en) 2023-10-01
AU2017357638B2 (en) 2023-11-16
KR20190072651A (ko) 2019-06-25
CN110249050B (zh) 2023-08-18
IL266318A (en) 2019-06-30
CN110249050A (zh) 2019-09-17
IL266318B1 (en) 2023-06-01

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