JP2020114185A - プロテオグリカン含有組成物の製造方法及びプロテオグリカン含有組成物 - Google Patents
プロテオグリカン含有組成物の製造方法及びプロテオグリカン含有組成物 Download PDFInfo
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- JP2020114185A JP2020114185A JP2019006386A JP2019006386A JP2020114185A JP 2020114185 A JP2020114185 A JP 2020114185A JP 2019006386 A JP2019006386 A JP 2019006386A JP 2019006386 A JP2019006386 A JP 2019006386A JP 2020114185 A JP2020114185 A JP 2020114185A
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Classifications
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4725—Proteoglycans, e.g. aggreccan
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0066—Isolation or extraction of proteoglycans from organs
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
- A23L29/281—Proteins, e.g. gelatin or collagen
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/48—Nerve growth factor [NGF]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Gastroenterology & Hepatology (AREA)
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- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Materials Engineering (AREA)
- Immunology (AREA)
- Birds (AREA)
- Dispersion Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Mycology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
水産加工工場から排出されるサケの頭部を入手し、その頭部から鼻軟骨を摘出して、サケ1匹分の頭部からおよそ28gの生軟骨を採取した。これを設定温度−30℃に設定した冷凍庫の庫内に静置して冷凍して、サケ鼻軟骨の凍結物を得た。このとき、凍結条件としては、緩慢凍結の条件(急速冷凍でなく)で行った。具体的には、原料の品温が氷結晶生成温度帯である−5℃以上0℃未満の温度帯を30分間程度かけて凍結させた。得られた凍結物を真空凍結乾燥装置にて凍結乾燥して、更に、ピンミル粉砕機(商品名「サンプルミル(SAM)」、奈良機械製作所製)を使用して、全体のおよそ90質量%以上が0.3mm経以上0.75mm径以下のスクリーンをパスするように粉砕した。得られた凍結乾燥粉砕物を純水(蒸留水)120mLに終濃度10w/v%となるように添加し、容器ごと11℃で15時間振盪させた。その後、遠心分離により固液分離して液部を回収し、真空乾燥装置にて乾燥して、乳白色の紛体状組成物を得た。
水産加工工場から排出されるサケの頭部を入手し、その頭部から鼻軟骨を摘出して、サケ44匹分の頭部からおよそ1kgの生軟骨を採取した。これをミートチョッパー装置(設定温度:10℃)にかけてミンチし、ペースト状のすり身を得た。得られたすり身は、品温が上がりすぎないよう注意しながら、純水(蒸留水)120mLに終濃度10w/v%となるように添加し、容器ごと11℃で15時間振盪させた。その後、遠心分離により固液分離して液部を回収し、真空乾燥装置にて乾燥して、乳白〜淡黄の色調の紛体状組成物を得た。
実施例1における凍結乾燥粉砕物からの抽出溶媒を、純水(蒸留水)ではなく0.005%NaOH水溶液(pH11.1)に代えた以外は、実施例1と同様にして、乳白色の紛体状組成物を得た。
採取した生軟骨を、一旦冷凍装置(設定温度:−25℃)にて急速冷凍し、これを10℃の流水で解凍して用いた以外は、実施例2と同様にして、紛体状組成物を調製した。得られた紛体状組成物の色調は、乳白〜淡黄であった。
実施例1〜3、比較例1で得られた紛体状組成物について、常法に従い、組成物中に含まれるプロテオグリカン量とその分子量について調べた。以下はHPLC分析による主な分析条件である。
・サイズ排除クロマトカラム: TSKgel G6000 PWXL(7.8mm×300mm)、排除限界5000万
・ガードカラム: TSKgel guardcolumn PWXL(6.0mm×40mm)
・カラム温度: 40℃
・移動相: 0.1M Phosphate buffer in 0.1MNaCl (pH7.0)
・流量: 0.4mL/min
・検出器: RI (示差屈折)
・定量用プロテオグリカン標品: Salmon Nasal Cartilage proteoglycan(コスモ・バイオ株式会社製)
・分子量用プルラン標品: STD P-800 Mw80.5×104、P-400 Mw36.6x104、P-200 Mw20.0×104(昭和電工株式会社製)
凍結条件の影響を調べた。具体的には、サケ氷頭(頭部の軟骨)の断片の生のものと、−20℃に急速冷凍したもの、実施例1,3の紛体状組成物の調製時と同じく、緩慢凍結の条件(−5℃以上0℃未満の温度帯を30分間程度かけて凍結)で冷凍したもの、のそれぞれの切片を作成し、プロテオグリカン染色試薬であるアルシャンブルー(青染色)で染色して、顕微鏡で観察した。
実施例1で得られた紛体状組成物について、アミノ酸組成分析を行った。アミノ酸組成分析は、常法に従い、試料を酸加水分解した後の加水分解物について、アミノ酸自動分析装置に供して、各アミノ酸量を測定した。その結果得られたヒドロキシプロリンの含有量(mg/100g)に12.51の換算係数を用いてコラーゲン量を算出したところ、コラーゲンの含有量は41質量%であった。
試験例1ではサイズ排除クロマトカラムを利用し、プルラン標品との相対比較で求められた分子量であったために誤差が大きいと考えられた。また、剛体球状、棒状、ランダムコイル状等の分子形状を予測することはできなかった。そこで、より絶対的な分子量測定法であり、分子形状に関する情報も得ることができる、SEC-MALS法を用いた静的光散乱法による構造解析を実施した。なお、SECはSize Exclusion Chromatograph(サイズ排除クロマトグラフ)の略であり、「MALS」はMulti Angle Light Scattering(多角度光散乱検出器)の略である。
・多角度光散乱検出器: DAWNHELEOSII(Wyatt Technology社製)
・サイズ排除クロマトカラム:ShodexSB-807 (排除限界5000万)
・検出器1:示差屈折率検出器OptilabT-rEX(Wyatt Technology社製)
・検出器2:粘度検出器ViscoStarIII(Wyatt Technology社製)
・移動相:0.1Mリン酸バッファー
・流量0.5mL/min
・温度40℃
・dn/dc値:0.16mg/L(文献値)
Claims (10)
- 魚由来の生軟骨を原料とし、前記原料を冷凍する冷凍工程と、前記冷凍工程で得られた凍結物を凍結乾燥する凍結乾燥工程とを含むことを特徴とするプロテオグリカン含有組成物の製造方法。
- 前記冷凍工程において、前記原料が−5℃以上0℃未満の温度帯を30分間以上経るようにして冷凍する、請求項1記載のプロテオグリカン含有組成物の製造方法。
- 前記凍結乾燥工程で得られた凍結乾燥物に、更に、水性溶媒を添加して抽出する抽出工程を含む、請求項1又は2記載のプロテオグリカン含有組成物の製造方法。
- 魚由来の生軟骨を原料とし、前記原料をすり身にするミンチ工程と、前記ミンチ工程で得られたすり身に水性溶媒を添加して抽出する抽出工程とを含むことを特徴とするプロテオグリカン含有組成物の製造方法。
- 前記抽出工程で得られた抽出物を、更に乾燥する乾燥工程を含む、請求項3又は4記載のプロテオグリカン含有組成物の製造方法。
- 前記乾燥工程で得られた乾燥物がプロテオグリカンを36質量%以上含有し、コラーゲンを36質量%以上含有する、請求項5記載のプロテオグリカン含有組成物の製造方法。
- 魚由来の軟骨抽出物からなり、プロテオグリカンを36質量%以上含有し、コラーゲンを36質量%以上含有し、前記プロテオグリカンと前記コラーゲンとの質量比が1:1.7〜1.25:1である、プロテオグリカン含有組成物。
- 前記プロテオグリカンの重量平均分子量は200〜415万ダルトンである、請求項7記載のプロテオグリカン含有組成物。
- 脂質の含有量が1質量%以下である、請求項7又は8記載のプロテオグリカン含有組成物。
- 前記重量平均分子量の200〜340万ダルトンの範囲に入るものが、30質量%以上を占める、請求項7〜9のいずれか1項に記載のプロテオグリカン含有組成物。
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MYPI2021003924A MY194247A (en) | 2019-01-17 | 2019-11-15 | Method for manufacturing proteoglycan-containing composition, and proteoglycan-containing composition |
US17/423,197 US20220089787A1 (en) | 2019-01-17 | 2019-11-15 | Method for manufacturing proteoglycan-containing composition, and proteoglycan-containing composition |
PCT/JP2019/044919 WO2020148989A1 (ja) | 2019-01-17 | 2019-11-15 | プロテオグリカン含有組成物の製造方法及びプロテオグリカン含有組成物 |
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KR20210110669A (ko) | 2021-09-08 |
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