JP2019522196A - 二重層形成のための浸透性不均衡法 - Google Patents
二重層形成のための浸透性不均衡法 Download PDFInfo
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- JP2019522196A JP2019522196A JP2018567730A JP2018567730A JP2019522196A JP 2019522196 A JP2019522196 A JP 2019522196A JP 2018567730 A JP2018567730 A JP 2018567730A JP 2018567730 A JP2018567730 A JP 2018567730A JP 2019522196 A JP2019522196 A JP 2019522196A
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Classifications
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- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G01N15/10—Investigating individual particles
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- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
- G01N15/131—Details
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Abstract
Description
[0058]図7は、システムの脂質二重層測定段階中の回路600の一部分の電気特性を表す電気モデル700を示す。図7に示すように、C2重層624は、C二重層626と直列に接続されているが、R二重層628(図6B参照のこと)は、電気モデル700から除去されている。R二重層628は、脂質膜/二重層に関連付けられた抵抗が、非常に高く、それゆえR二重層が、開回路として近似され得るので、電気モデル700から除去することができる。図7に示すように、C2重層624およびC二重層626は、ncap608と直列にさらに接続される。
Z(ncap)は、ncapに関連付けられた交流インピーダンスであり、
Z(2重層)は、作用電極に関連付けられた交流インピーダンスであり、
Z(二重層)は、脂質膜/二重層に関連付けられた交流インピーダンスである。
C(二重層)は、脂質膜/二重層に関連付けられたキャパシタンスである。
ΔVliqは、バルク液体に印加される電圧変化である。
C(ncap)は、ncapに関連付けられたキャパシタンスであり、
C(二重層)は、脂質膜/二重層に関連付けられたキャパシタンスである。
Claims (15)
- ナノポアベースの配列決定チップ内のセルアレイ上に複数の脂質二重層を形成する方法であって、
前記セルの各々は、ウェルを備え、
前記方法が:
第1の浸透圧モル濃度を有する第1の塩緩衝溶液をナノポアベースの配列決定チップ内のセル上に流し、前記セル内のウェルを前記第1の塩緩衝溶液で実質的に充填するステップ;
脂質および溶媒の混合物を前記セル上に流し、前記ウェル内に前記第1の塩緩衝溶液を封入する前記ウェル上に脂質膜を堆積させるステップ;ならびに、
第2の浸透圧モル濃度を有する第2の塩緩衝溶液を前記ウェル上に流し、前記脂質膜の厚さを減少させるステップであって、ここで前記第2の浸透圧モル濃度が前記第1の浸透圧モル濃度より低い浸透圧モル濃度であり、その結果、浸透性不均衡が前記ウェルの内側の第1の体積と前記ウェルの外側の第2の体積との間に形成される;
を含む、前記方法。 - 前記浸透性不均衡が、水を前記脂質膜を通り前記ウェルへと拡散させ、前記脂質膜を上方へ反らせる、請求項1に記載の方法。
- 脂質二重層開始刺激を印加し、前記脂質膜上への小さな脂質二重層の形成を促進するステップをさらに含む、請求項1に記載の方法。
- 前記脂質二重層開始刺激を印加する前記ステップが、時間の経過につれて数サイクル実行され、前記脂質二重層開始刺激のレベルが、前記数サイクルで適応可能なものである、請求項3に記載の方法。
- 前記脂質二重層開始刺激が、振動刺激を含む、請求項3に記載の方法。
- 前記振動刺激を印加するステップが、前記ウェルの外側の前記第2の体積内で波を発生させるステップを含む、請求項5に記載の方法。
- 前記脂質二重層開始刺激が、電気的な刺激を含む、請求項3に記載の方法。
- 前記第2の浸透圧モル濃度を有する前記第2の塩緩衝溶液を前記ウェル上に流す前記ステップが、時間の経過につれて数サイクル実行され、前記第2の浸透圧モル濃度は、前記数サイクルで次第に増大される、請求項1に記載の方法。
- ナノポアベースの配列決定チップ内のセルアレイ上に複数の脂質二重層を形成する装置であって、
システムが:
セルアレイを含むナノポアベースの配列決定チップであって、前記セルの各々がウェルを含む前記ナノポアベースの配列決定チップ;
前記ナノポアベースの配列決定チップに連結された流動室;および、
プロセッサまたは回路;
を備え、
前記プロセッサまたは回路は、
第1の浸透圧モル濃度を有する第1の塩緩衝溶液を前記ナノポアベースの配列決定チップ内のセル上に流し、前記セル内のウェルを前記第1の塩緩衝溶液で実質的に充填するように構成され、
脂質および溶媒の混合物を前記セル上に流し、前記ウェル内に前記第1の塩緩衝溶液を封入する前記ウェル上に脂質膜を堆積させるように構成され、
第2の浸透圧モル濃度を有する第2の塩緩衝溶液を前記ウェル上に流し、前記脂質膜の厚さを減少させるように構成され、ここで前記第2の浸透圧モル濃度が前記第1の浸透圧モル濃度より低い浸透圧モル濃度であり、その結果、浸透性不均衡が前記ウェルの内側の第1の体積と前記ウェルの外側の第2の体積との間に形成される、
前記装置。 - 前記浸透性不均衡が、水を前記脂質膜を通り前記ウェルへと拡散させ、前記脂質膜を上方へ反らせる、請求項9に記載の装置。
- 前記プロセッサまたは回路が、脂質二重層開始刺激を印加し、前記脂質膜上への小さな脂質二重層の形成を促進するようにさらに構成された、請求項9に記載の装置。
- 前記脂質二重層開始刺激を印加する前記ステップが、時間の経過につれて数サイクル実行され、前記脂質二重層開始刺激のレベルが、前記数サイクルで適応可能なものである、請求項11に記載の装置。
- 前記脂質二重層開始刺激が、振動刺激を含む、請求項11に記載の装置。
- 前記脂質二重層開始刺激が、電気的な刺激を含む、請求項11に記載の装置。
- 前記第2の浸透圧モル濃度を有する前記第2の塩緩衝溶液を前記ウェル上に流す前記ステップが、時間の経過につれて数サイクル実行され、前記第2の浸透圧モル濃度は、前記数サイクルで次第に増大される、請求項9に記載のシステム。
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