JP2019530861A - ハイブリッドモード刺激を伴う電圧モードを用いたナノポアベースの配列決定 - Google Patents
ハイブリッドモード刺激を伴う電圧モードを用いたナノポアベースの配列決定 Download PDFInfo
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- JP2019530861A JP2019530861A JP2019514075A JP2019514075A JP2019530861A JP 2019530861 A JP2019530861 A JP 2019530861A JP 2019514075 A JP2019514075 A JP 2019514075A JP 2019514075 A JP2019514075 A JP 2019514075A JP 2019530861 A JP2019530861 A JP 2019530861A
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- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G01N27/44713—Particularly adapted electric power supply
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Abstract
Description
Claims (15)
- ナノポアで分子を分析するための方法であって、
液体電圧を脂質二重層の第1面側の電解質に印加するステップであって、前記液体電圧が、前記分子のタグまたは前記分子の一部を前記脂質二重層内のナノポア内に捕捉する傾向を有するタグ読取り電圧レベルを伴うタグ読取り期間と、前記分子の前記タグまたは前記一部を前記脂質二重層内の前記ナノポアから放出する傾向を有する開放チャネル電圧レベルを伴う開放チャネル期間とを含む、前記ステップ、
予備充電時間中、予備充電電圧源を、積分コンデンサと、前記脂質二重層の第2面側の作用電極とに接続するステップであって、それにより前記積分コンデンサおよび前記作用電極が、予備充電電圧に充電される前記ステップ、ならびに、
積分期間中、前記予備充電電圧源を、前記積分コンデンサおよび前記作用電極から切断するステップであって、それにより前記積分コンデンサの電圧および前記作用電極の電圧は、電流が前記脂質二重層内の前記ナノポアを通って流れるとき変化し得る前記ステップ、
を含み、
前記予備充電期間が、前記タグ読取り期間の開始部分と重複する、
前記方法。 - 前記予備充電期間が、前記開放チャネル期間の終了部分と重複する、請求項1に記載の方法。
- 前記予備充電期間が、前記開放チャネル期間から前記タグ読取り期間への遷移全体に及ぶ、請求項1に記載の方法。
- 前記予備充電期間が、前記開放チャネル期間の開始部分と重複する、請求項1に記載の方法。
- 前記予備充電期間が、前記タグ読取り期間の終了部分と重複する、請求項1に記載の方法。
- 前記予備充電期間が、前記タグ読取り期間から前記開放チャネル期間への遷移全体に及ぶ、請求項1に記載の方法。
- 前記予備充電期間が、100マイクロ秒〜50ミリ秒の持続時間を有する、請求項3または6に記載の方法。
- 前記予備充電期間が、50マイクロ秒〜25ミリ秒の持続時間を有する、請求項1、2、4、または5のいずれか1項に記載の方法。
- 分子を分析するためのシステム、機器、またはデバイスであって、
電圧源、
前記電圧源に結合された対電極、
予備充電電圧源、
前記予備充電電圧源と接続するように構成可能である積分コンデンサ、
前記予備充電電圧源と接続するように構成可能である作用電極、および、
コントローラ、
を備え、
前記コントローラは、液体電圧を、前記対電極を介して、脂質二重層の第1面側の電解質に印加するために前記電圧源を制御するように構成され、前記液体電圧が、前記分子のタグまたは前記分子の一部を前記脂質二重層内のナノポア内に捕捉する傾向を有するタグ読取り電圧レベルを伴うタグ読取り期間と、前記分子の前記タグまたは前記一部を前記脂質二重層内の前記ナノポアから放出する傾向を有する開放チャネル電圧レベルを伴う開放チャネル期間とを含み、
前記コントローラは、予備充電時間中、予備充電電圧源を、前記積分コンデンサと、前記脂質二重層の第2の側面上の前記作用電極とに接続し、その結果前記積分コンデンサおよび前記作用電極が、予備充電電圧に充電されるように構成され、
前記コントローラは、積分期間中、前記予備充電電圧源を、前記積分コンデンサおよび前記作用電極から切断し、その結果前記積分コンデンサの電圧および前記作用電極の電圧は、電流が前記脂質二重層内の前記ナノポアを通って流れるとき変化し得るように構成され、
前記予備充電期間が、前記タグ読取り期間の開始部分と重複し、
さらに、前記コントローラに結合され、前記コントローラに命令を供給するように構成されたメモリを備える、
前記システム、機器、またはデバイス。 - 前記予備充電期間が、前記開放チャネル期間の終了部分と重複する、請求項9に記載のシステム、機器、またはデバイス。
- 前記予備充電期間が、前記開放チャネル期間から前記タグ読取り期間への遷移全体に及ぶ、請求項9に記載のシステム、機器、またはデバイス。
- 前記予備充電期間が、前記開放チャネル期間の開始部分と重複する、請求項9に記載のシステム、機器、またはデバイス。
- 前記予備充電期間が、前記タグ読取り期間の終了部分と重複する、請求項9に記載のシステム、機器、またはデバイス。
- 前記予備充電期間が、前記タグ読取り期間から前記開放チャネル期間への遷移全体に及ぶ、請求項9に記載のシステム、機器、またはデバイス。
- 前記予備充電期間が、100マイクロ秒〜25ミリ秒の持続時間を有する、請求項9〜14のいずれかに記載のシステム、機器、またはデバイス。
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