JP2019516371A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2019516371A5 JP2019516371A5 JP2018558148A JP2018558148A JP2019516371A5 JP 2019516371 A5 JP2019516371 A5 JP 2019516371A5 JP 2018558148 A JP2018558148 A JP 2018558148A JP 2018558148 A JP2018558148 A JP 2018558148A JP 2019516371 A5 JP2019516371 A5 JP 2019516371A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinant
- sequence
- nucleic acid
- host cell
- recombinant protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 40
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 40
- 150000007523 nucleic acids Chemical class 0.000 claims description 29
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 25
- 210000004027 cell Anatomy 0.000 claims description 22
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 19
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 11
- 238000012217 deletion Methods 0.000 claims description 11
- 230000037430 deletion Effects 0.000 claims description 11
- 238000003780 insertion Methods 0.000 claims description 11
- 230000037431 insertion Effects 0.000 claims description 11
- 238000006467 substitution reaction Methods 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 6
- 239000013604 expression vector Substances 0.000 claims description 6
- 238000003259 recombinant expression Methods 0.000 claims description 6
- 210000004899 c-terminal region Anatomy 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 5
- 108020004705 Codon Proteins 0.000 claims description 4
- 239000006143 cell culture medium Substances 0.000 claims description 4
- 210000001322 periplasm Anatomy 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims description 2
- 102000025171 antigen binding proteins Human genes 0.000 claims description 2
- 108091000831 antigen binding proteins Proteins 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims 1
- 230000006798 recombination Effects 0.000 claims 1
- 238000000746 purification Methods 0.000 description 3
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 238000011210 chromatographic step Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022015944A JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662332636P | 2016-05-06 | 2016-05-06 | |
| US62/332,636 | 2016-05-06 | ||
| US201662338045P | 2016-05-18 | 2016-05-18 | |
| US62/338,045 | 2016-05-18 | ||
| PCT/EP2017/060654 WO2017191255A1 (en) | 2016-05-06 | 2017-05-04 | Method of producing a recombinant protein |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022015944A Division JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019516371A JP2019516371A (ja) | 2019-06-20 |
| JP2019516371A5 true JP2019516371A5 (https=) | 2020-05-28 |
Family
ID=58765814
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018558148A Pending JP2019516371A (ja) | 2016-05-06 | 2017-05-04 | 組み換えタンパク質の生成方法 |
| JP2022015944A Pending JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022015944A Pending JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US11098312B2 (https=) |
| EP (1) | EP3452496A1 (https=) |
| JP (2) | JP2019516371A (https=) |
| KR (1) | KR102504317B1 (https=) |
| CN (1) | CN109071611B (https=) |
| AU (1) | AU2017260277A1 (https=) |
| BR (1) | BR112018072043A2 (https=) |
| CA (1) | CA3023025A1 (https=) |
| IL (1) | IL262544A (https=) |
| MX (1) | MX2018013562A (https=) |
| RU (1) | RU2742006C2 (https=) |
| SG (1) | SG11201808976WA (https=) |
| TW (1) | TWI794170B (https=) |
| WO (1) | WO2017191255A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7517707B2 (ja) * | 2019-02-15 | 2024-07-17 | オンコシミス バイオテック プライベート リミテッド | タンパク質分泌増強のための細菌発現ベクター |
| KR102094480B1 (ko) | 2019-05-27 | 2020-04-01 | (주)엘에스케이화인텍스 | 재사용 가능한 미세 먼지 항균 마스크 |
| CN110551199B (zh) * | 2019-09-26 | 2022-01-25 | 杭州纽龙日尚生物制品有限公司 | 一种提高工程菌分泌表达重组人表皮生长因子的方法 |
| KR20240021234A (ko) * | 2021-06-10 | 2024-02-16 | 펠리칸 테크놀로지 홀딩스, 인크. | 재조합 단백질 발현을 위한 박테리아 숙주 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005538731A (ja) * | 2002-09-20 | 2005-12-22 | シーワイエム１ピー エイ／エス | 宿主細胞からの組換えポリペプチドの産生を高める方法 |
| GB0426160D0 (en) * | 2004-11-29 | 2004-12-29 | Era Plantech S L | Production of proteins |
| WO2007089093A1 (en) * | 2006-01-31 | 2007-08-09 | Republic Of Korea (Republic Of National Fisheries Research And Development Institute) | Production of a soluble native form of recombinant protein by the signal sequence and secretional enhancer |
| GB0602173D0 (en) | 2006-02-03 | 2006-03-15 | Avecia Ltd | Expression system |
| EP2176291A1 (en) * | 2007-08-10 | 2010-04-21 | Wacker Chemie AG | Expression of full length igg and secretion into the culture medium of prokaryotic cells |
| RU2488635C1 (ru) | 2012-03-22 | 2013-07-27 | Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития | Способ получения рекомбинантного антиангиогенного полипептида |
| US20140244228A1 (en) * | 2012-09-19 | 2014-08-28 | Agency For Science, Technology And Research | Codon optimization of a synthetic gene(s) for protein expression |
-
2017
- 2017-05-04 WO PCT/EP2017/060654 patent/WO2017191255A1/en not_active Ceased
- 2017-05-04 MX MX2018013562A patent/MX2018013562A/es unknown
- 2017-05-04 EP EP17725188.1A patent/EP3452496A1/en not_active Withdrawn
- 2017-05-04 RU RU2018137305A patent/RU2742006C2/ru active
- 2017-05-04 JP JP2018558148A patent/JP2019516371A/ja active Pending
- 2017-05-04 KR KR1020187034736A patent/KR102504317B1/ko active Active
- 2017-05-04 BR BR112018072043-7A patent/BR112018072043A2/pt not_active Application Discontinuation
- 2017-05-04 SG SG11201808976WA patent/SG11201808976WA/en unknown
- 2017-05-04 TW TW106114729A patent/TWI794170B/zh active
- 2017-05-04 US US16/098,890 patent/US11098312B2/en active Active
- 2017-05-04 AU AU2017260277A patent/AU2017260277A1/en not_active Abandoned
- 2017-05-04 CN CN201780028031.XA patent/CN109071611B/zh not_active Expired - Fee Related
- 2017-05-04 CA CA3023025A patent/CA3023025A1/en not_active Abandoned
-
2018
- 2018-10-24 IL IL262544A patent/IL262544A/en unknown
-
2022
- 2022-02-03 JP JP2022015944A patent/JP2022070902A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI4011908T3 (fi) | Ultrapitkävaikutteiset insuliini-Fc-fuusioproteiinit ja niiden käyttömenetelmät | |
| JP2019516371A5 (https=) | ||
| JP2022062113A5 (https=) | ||
| JP5698536B2 (ja) | アフィニティタグが結合した融合コラゲナーゼおよびその製造法 | |
| RU2014141505A (ru) | Способ получения поверхностно-активных пептидов | |
| JP2022070902A5 (https=) | ||
| JP2011224002A5 (https=) | ||
| FI3889173T3 (fi) | Optimoitu tekijä viii:n geeni | |
| ATE421977T1 (de) | Reinigung von peptiden unter verwendung von metall-ion-affinitätschromatographie | |
| NO20090451L (no) | Fremgangsmate for fremstilling av konjugater av insulin-lignende vekst faktor-1 og poly(etylenglukol) | |
| CN104487454A (zh) | 用于蛋白质表达的重组细菌宿主细胞 | |
| JP2020529221A5 (https=) | ||
| JP5307716B2 (ja) | ドックリンポリペプチドおよびそれを利用した組換え融合タンパク質の精製方法 | |
| JP2018524019A5 (https=) | ||
| CN104593313A (zh) | 用于制备细菌素durancin GL的重组菌、制备方法及应用 | |
| CN109456989B (zh) | 一种提高毕赤酵母分泌表达的载体的构建方法 | |
| KR100961528B1 (ko) | 대장균에서 활성형 인간 상피세포 성장인자의 대량제조방법 | |
| RU2018137305A (ru) | Способ продуцирования рекомбинантного белка | |
| DK1819816T3 (da) | Fremgangsmåder til fremstilling og udskillelse af modificerede peptider | |
| CN103725705A (zh) | 一种通用型重组表达载体及其构建方法和应用 | |
| CN105296506A (zh) | 以硫磺菌蘑菇凝集素n-乙酰氨基乳糖胺结合域作为融合标签的目标蛋白表达与纯化方法 | |
| US20160024509A1 (en) | Expression Vector | |
| KR930008147A (ko) | 화학 화합물의 생합성적 제조방법 | |
| WO2010082097A4 (pt) | Proteínas de fusão, processo para a sua preparação e sua utilização em sistemas de expressão de proteínas recombinantes | |
| CN110387366A (zh) | 一种aep环化酶在毕赤酵母中的表达方法及其应用 |