JP2019515674A5 - - Google Patents

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JP2019515674A5
JP2019515674A5 JP2018554729A JP2018554729A JP2019515674A5 JP 2019515674 A5 JP2019515674 A5 JP 2019515674A5 JP 2018554729 A JP2018554729 A JP 2018554729A JP 2018554729 A JP2018554729 A JP 2018554729A JP 2019515674 A5 JP2019515674 A5 JP 2019515674A5
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JP
Japan
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conjugating
oligonucleotides
rna
cells
dna
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JP2018554729A
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Japanese (ja)
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JP2019515674A (ja
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Priority claimed from PCT/US2017/028329 external-priority patent/WO2017184707A1/en
Publication of JP2019515674A publication Critical patent/JP2019515674A/ja
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JP2018554729A 2016-04-19 2017-04-19 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法 Pending JP2019515674A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662324407P 2016-04-19 2016-04-19
US62/324,407 2016-04-19
PCT/US2017/028329 WO2017184707A1 (en) 2016-04-19 2017-04-19 Immobilization-based systems and methods for genetic analysis and other applications

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JP2019515674A JP2019515674A (ja) 2019-06-13
JP2019515674A5 true JP2019515674A5 (enExample) 2020-05-28

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JP2018554729A Pending JP2019515674A (ja) 2016-04-19 2017-04-19 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法

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US (2) US20190127789A1 (enExample)
JP (1) JP2019515674A (enExample)
CA (1) CA3021735A1 (enExample)
WO (1) WO2017184707A1 (enExample)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014028378A2 (en) 2012-08-13 2014-02-20 The Regents Of The University Of California Methods and systems for detecting biological components
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
EP3160654A4 (en) 2014-06-27 2017-11-15 The Regents of The University of California Pcr-activated sorting (pas)
US10434507B2 (en) 2014-10-22 2019-10-08 The Regents Of The University Of California High definition microdroplet printer
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CN107530654A (zh) 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
CN116064731A (zh) 2015-03-13 2023-05-05 哈佛学院院长及董事 使用扩增测定细胞
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
WO2017184707A1 (en) 2016-04-19 2017-10-26 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
AU2017382905A1 (en) * 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
IL302912A (en) 2016-12-22 2023-07-01 Guardant Health Inc Methods and systems for analyzing nucleic acid molecules
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
WO2018145041A1 (en) 2017-02-06 2018-08-09 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3625353B1 (en) 2017-05-18 2022-11-30 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
CA3106307A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
EP3821032B1 (en) * 2018-07-13 2026-03-11 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020154391A1 (en) * 2019-01-22 2020-07-30 Mission Bio Methods of nucleic acid detection and primer design
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
WO2020218551A1 (ja) * 2019-04-26 2020-10-29 bitBiome株式会社 ゲルカプセル方式による1細胞ゲノムライブラリーからの配列スクリーニング法
WO2020218549A1 (ja) * 2019-04-26 2020-10-29 bitBiome株式会社 単一生物単位の遺伝情報またはその他の生体分子の情報を使用した、サンプルのスクリーニング法
AU2020280104A1 (en) * 2019-05-22 2022-01-20 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
EP4041919A1 (en) 2019-09-30 2022-08-17 Guardant Health, Inc. Compositions and methods for analyzing cell-free dna in methylation partitioning assays
JP2023511440A (ja) * 2020-01-22 2023-03-17 アトレカ インコーポレイテッド 複数の転写産物のハイスループット連結
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US20230304069A1 (en) * 2020-08-13 2023-09-28 The Johns Hopkins University Methods and compositions for single cell analysis
WO2022087309A1 (en) 2020-10-23 2022-04-28 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and base conversion
US12655450B1 (en) 2020-10-27 2026-06-16 10X Genomics, Inc. Methods, systems, and kits for detection of nucleic acid molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20220325277A1 (en) * 2021-04-13 2022-10-13 Inscripta, Inc. Genomic edit detection at the single cell level
GB202418827D0 (en) * 2024-12-20 2025-02-05 Univ Oxford Innovation Ltd Sequencing method

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8928874D0 (en) 1989-12-21 1990-02-28 Celltech Ltd Humanised antibodies
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
CN1267565C (zh) 2003-06-09 2006-08-02 清华大学 微液滴振荡型硅槽式聚合酶链式反应生物芯片
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2008005675A2 (en) 2006-06-30 2008-01-10 Applera Corporation Emulsion pcr and amplicon capture
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US8232049B2 (en) * 2006-09-29 2012-07-31 Case Western Reserve University Method for detecting small oligonucleotides
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
JP2008245612A (ja) 2007-03-30 2008-10-16 Hitachi Ltd 試料調製法および装置
US9664619B2 (en) 2008-04-28 2017-05-30 President And Fellows Of Harvard College Microfluidic device for storage and well-defined arrangement of droplets
EP2307560A2 (en) 2008-06-27 2011-04-13 Massachusetts Institute of Technology Microfluidic droplets for metabolic engineering and other applications
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
CA2766795C (en) 2009-06-26 2017-10-03 President And Fellows Of Harvard College Fluid injection
US8609339B2 (en) 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9902950B2 (en) 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding
WO2012083225A2 (en) 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
CA2823193C (en) 2010-12-27 2017-06-06 Ibis Biosciences, Inc. Quantitating high titer samples by digital pcr
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing
GB201108259D0 (en) * 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
US20130189700A1 (en) 2011-07-25 2013-07-25 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
CN102260747B (zh) 2011-08-16 2013-02-13 杭州电子科技大学 生物芯片上基于微乳液等温扩增的dna测序方法
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
CN104471077B (zh) 2012-05-21 2017-05-24 富鲁达公司 颗粒群的单颗粒分析
US9527049B2 (en) 2012-06-20 2016-12-27 Bio-Rad Laboratories, Inc. Stabilized droplets for calibration and testing
CN104781386B (zh) 2012-09-12 2018-04-06 基纽拜奥股份有限公司 用于进行试验的集成微流体系统、方法和试剂盒
WO2014047561A1 (en) 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
KR20230003659A (ko) * 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
WO2014145555A1 (en) 2013-03-15 2014-09-18 Lariat Biosciences, Inc. Microfluidic methods for manipulating dna
KR102402451B1 (ko) 2013-03-28 2022-05-26 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
US10151429B2 (en) 2013-05-14 2018-12-11 President And Fellows Of Harvard College Rapid production of droplets
CN105431553B (zh) 2013-05-29 2020-02-07 生物辐射实验室股份有限公司 用于在基于乳液的微流体中测序的系统和方法
EP3296408A1 (en) 2013-09-05 2018-03-21 The Jackson Laboratory Compositions for rna-chromatin interaction analysis and uses thereof
US10947587B2 (en) 2013-11-05 2021-03-16 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
JP6608368B2 (ja) 2013-12-30 2019-11-20 アトレカ インコーポレイテッド 核酸バーコードを用いた単一細胞と関連づけられた核酸の分析方法
WO2015160919A1 (en) 2014-04-16 2015-10-22 President And Fellows Of Harvard College Systems and methods for producing droplet emulsions with relatively thin shells
US20170029813A1 (en) 2014-04-17 2017-02-02 President And Fellows Of Harvard College Methods and systems for droplet tagging and amplification
CA2945794A1 (en) 2014-04-17 2015-10-22 President And Fellows Of Harvard College Systems and methods for droplet tagging
EP3134536B1 (en) 2014-04-21 2019-12-18 President and Fellows of Harvard College Systems and methods for barcoding nucleic acids
EP4574974A3 (en) * 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
JP2017532024A (ja) 2014-09-09 2017-11-02 ザ・ブロード・インスティテュート・インコーポレイテッド コンポジット単一細胞核酸分析のための液滴ベースの方法および機器
WO2016118870A1 (en) 2015-01-23 2016-07-28 President And Fellows Of Harvard College Systems, methods, and kits for amplifying or cloning within droplets
CN107530654A (zh) 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
CN116064731A (zh) 2015-03-13 2023-05-05 哈佛学院院长及董事 使用扩增测定细胞
CN107614684A (zh) 2015-04-17 2018-01-19 哈佛学院院长及董事 用于基因测序和其它应用的条形编码系统及方法
CN108430617A (zh) 2015-12-30 2018-08-21 生物辐射实验室股份有限公司 液滴划分的基于pcr的文库制备
WO2017184707A1 (en) 2016-04-19 2017-10-26 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
EP4090740A4 (en) 2020-01-14 2024-01-03 President and Fellows of Harvard College Devices and methods for determining nucleic acids using digital droplet pcr and related techniques
US12692558B2 (en) 2020-08-28 2026-07-28 The Regents Of The University Of California Point of care and improved detection and quantification of biomolecules

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