CA3021735A1 - Immobilization-based systems and methods for genetic analysis and other applications - Google Patents
Immobilization-based systems and methods for genetic analysis and other applications Download PDFInfo
- Publication number
- CA3021735A1 CA3021735A1 CA3021735A CA3021735A CA3021735A1 CA 3021735 A1 CA3021735 A1 CA 3021735A1 CA 3021735 A CA3021735 A CA 3021735A CA 3021735 A CA3021735 A CA 3021735A CA 3021735 A1 CA3021735 A1 CA 3021735A1
- Authority
- CA
- Canada
- Prior art keywords
- oligonucleotides
- particles
- droplet
- droplets
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662324407P | 2016-04-19 | 2016-04-19 | |
| US62/324,407 | 2016-04-19 | ||
| PCT/US2017/028329 WO2017184707A1 (en) | 2016-04-19 | 2017-04-19 | Immobilization-based systems and methods for genetic analysis and other applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3021735A1 true CA3021735A1 (en) | 2017-10-26 |
Family
ID=60116460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3021735A Pending CA3021735A1 (en) | 2016-04-19 | 2017-04-19 | Immobilization-based systems and methods for genetic analysis and other applications |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US20190127789A1 (enExample) |
| JP (1) | JP2019515674A (enExample) |
| CA (1) | CA3021735A1 (enExample) |
| WO (1) | WO2017184707A1 (enExample) |
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| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN114891871A (zh) | 2012-08-14 | 2022-08-12 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
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| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
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| EP3160654A4 (en) | 2014-06-27 | 2017-11-15 | The Regents of The University of California | Pcr-activated sorting (pas) |
| US10434507B2 (en) | 2014-10-22 | 2019-10-08 | The Regents Of The University Of California | High definition microdroplet printer |
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| CN116064731A (zh) | 2015-03-13 | 2023-05-05 | 哈佛学院院长及董事 | 使用扩增测定细胞 |
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| JP2017532024A (ja) | 2014-09-09 | 2017-11-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | コンポジット単一細胞核酸分析のための液滴ベースの方法および機器 |
| WO2016118870A1 (en) | 2015-01-23 | 2016-07-28 | President And Fellows Of Harvard College | Systems, methods, and kits for amplifying or cloning within droplets |
| CN107530654A (zh) | 2015-02-04 | 2018-01-02 | 加利福尼亚大学董事会 | 通过在离散实体中条形码化对核酸进行测序 |
| CN116064731A (zh) | 2015-03-13 | 2023-05-05 | 哈佛学院院长及董事 | 使用扩增测定细胞 |
| CN107614684A (zh) | 2015-04-17 | 2018-01-19 | 哈佛学院院长及董事 | 用于基因测序和其它应用的条形编码系统及方法 |
| CN108430617A (zh) | 2015-12-30 | 2018-08-21 | 生物辐射实验室股份有限公司 | 液滴划分的基于pcr的文库制备 |
| WO2017184707A1 (en) | 2016-04-19 | 2017-10-26 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| US10549279B2 (en) | 2017-08-22 | 2020-02-04 | 10X Genomics, Inc. | Devices having a plurality of droplet formation regions |
| EP4090740A4 (en) | 2020-01-14 | 2024-01-03 | President and Fellows of Harvard College | Devices and methods for determining nucleic acids using digital droplet pcr and related techniques |
| US12692558B2 (en) | 2020-08-28 | 2026-07-28 | The Regents Of The University Of California | Point of care and improved detection and quantification of biomolecules |
-
2017
- 2017-04-19 WO PCT/US2017/028329 patent/WO2017184707A1/en not_active Ceased
- 2017-04-19 CA CA3021735A patent/CA3021735A1/en active Pending
- 2017-04-19 US US16/094,766 patent/US20190127789A1/en not_active Abandoned
- 2017-04-19 JP JP2018554729A patent/JP2019515674A/ja active Pending
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2022
- 2022-10-24 US US17/972,442 patent/US12545953B2/en active Active
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|---|---|
| WO2017184707A1 (en) | 2017-10-26 |
| US12545953B2 (en) | 2026-02-10 |
| US20190127789A1 (en) | 2019-05-02 |
| US20250215485A1 (en) | 2025-07-03 |
| JP2019515674A (ja) | 2019-06-13 |
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