CA3021735A1 - Immobilization-based systems and methods for genetic analysis and other applications - Google Patents
Immobilization-based systems and methods for genetic analysis and other applications Download PDFInfo
- Publication number
- CA3021735A1 CA3021735A1 CA3021735A CA3021735A CA3021735A1 CA 3021735 A1 CA3021735 A1 CA 3021735A1 CA 3021735 A CA3021735 A CA 3021735A CA 3021735 A CA3021735 A CA 3021735A CA 3021735 A1 CA3021735 A1 CA 3021735A1
- Authority
- CA
- Canada
- Prior art keywords
- oligonucleotides
- particles
- droplet
- droplets
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662324407P | 2016-04-19 | 2016-04-19 | |
| US62/324,407 | 2016-04-19 | ||
| PCT/US2017/028329 WO2017184707A1 (en) | 2016-04-19 | 2017-04-19 | Immobilization-based systems and methods for genetic analysis and other applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3021735A1 true CA3021735A1 (en) | 2017-10-26 |
Family
ID=60116460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3021735A Pending CA3021735A1 (en) | 2016-04-19 | 2017-04-19 | Immobilization-based systems and methods for genetic analysis and other applications |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US20190127789A1 (enExample) |
| JP (1) | JP2019515674A (enExample) |
| CA (1) | CA3021735A1 (enExample) |
| WO (1) | WO2017184707A1 (enExample) |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2519906B (en) | 2012-08-13 | 2017-02-08 | Univ California | Methods for detecting target nucleic acids in sample lysate droplets |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| AU2015279548B2 (en) | 2014-06-26 | 2020-02-27 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US10697007B2 (en) | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
| CA3001986C (en) | 2014-10-22 | 2023-02-21 | The Regents Of The University Of California | High definition microdroplet printer |
| US11111519B2 (en) | 2015-02-04 | 2021-09-07 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
| CN116064731A (zh) | 2015-03-13 | 2023-05-05 | 哈佛学院院长及董事 | 使用扩增测定细胞 |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| WO2018031691A1 (en) | 2016-08-10 | 2018-02-15 | The Regents Of The University Of California | Combined multiple-displacement amplification and pcr in an emulsion microdroplet |
| CA3047328A1 (en) * | 2016-12-21 | 2018-06-28 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| IL302912A (en) | 2016-12-22 | 2023-07-01 | Guardant Health Inc | Methods and systems for analyzing nucleic acid molecules |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3545089B1 (en) | 2017-01-30 | 2022-03-09 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| EP3625353B1 (en) | 2017-05-18 | 2022-11-30 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US10821442B2 (en) | 2017-08-22 | 2020-11-03 | 10X Genomics, Inc. | Devices, systems, and kits for forming droplets |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| US10501739B2 (en) | 2017-10-18 | 2019-12-10 | Mission Bio, Inc. | Method, systems and apparatus for single cell analysis |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| CN111479631B (zh) | 2017-10-27 | 2022-02-22 | 10X基因组学有限公司 | 用于样品制备和分析的方法和系统 |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| CN118818037A (zh) | 2017-12-12 | 2024-10-22 | 10X基因组学有限公司 | 用于单细胞处理的系统和方法 |
| CN111712579B (zh) | 2017-12-22 | 2024-10-15 | 10X基因组学有限公司 | 用于处理来自一个或多个细胞的核酸分子的系统和方法 |
| WO2019157529A1 (en) | 2018-02-12 | 2019-08-15 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2019169028A1 (en) | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| JP2021531824A (ja) * | 2018-07-13 | 2021-11-25 | ハイファイバイオ エスアエス | 患者層別化のための液滴単一細胞エピゲノムプロファイリングの使用 |
| EP3821032A1 (en) * | 2018-07-13 | 2021-05-19 | HiFiBiO SAS | Use of droplet single cell epigenome profiling for patient stratification |
| US12188014B1 (en) | 2018-07-25 | 2025-01-07 | 10X Genomics, Inc. | Compositions and methods for nucleic acid processing using blocking agents |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| EP3830289A1 (en) | 2018-08-03 | 2021-06-09 | 10X Genomics, Inc. | Methods and systems to minimize barcode exchange |
| WO2020041148A1 (en) | 2018-08-20 | 2020-02-27 | 10X Genomics, Inc. | Methods and systems for detection of protein-dna interactions using proximity ligation |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| US12169198B2 (en) | 2019-01-08 | 2024-12-17 | 10X Genomics, Inc. | Systems and methods for sample analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| AU2020212984A1 (en) * | 2019-01-22 | 2021-08-26 | Mission Bio, Inc. | Methods of nucleic acid detection and primer design |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| US12305239B2 (en) | 2019-02-12 | 2025-05-20 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2020167862A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transfer of reagents between droplets |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| US12275993B2 (en) | 2019-02-12 | 2025-04-15 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
| WO2020185791A1 (en) | 2019-03-11 | 2020-09-17 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
| JPWO2020218549A1 (enExample) * | 2019-04-26 | 2020-10-29 | ||
| JPWO2020218551A1 (enExample) * | 2019-04-26 | 2020-10-29 | ||
| US11365441B2 (en) * | 2019-05-22 | 2022-06-21 | Mission Bio, Inc. | Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| CN114616343A (zh) | 2019-09-30 | 2022-06-10 | 夸登特健康公司 | 用于在甲基化分区测定中分析无细胞dna的组合物和方法 |
| EP4093869A1 (en) * | 2020-01-22 | 2022-11-30 | Atreca, Inc. | High throughput linking of multiple transcripts |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| WO2022087309A1 (en) | 2020-10-23 | 2022-04-28 | Guardant Health, Inc. | Compositions and methods for analyzing dna using partitioning and base conversion |
| US12480158B1 (en) | 2020-11-05 | 2025-11-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12084715B1 (en) | 2020-11-05 | 2024-09-10 | 10X Genomics, Inc. | Methods and systems for reducing artifactual antisense products |
| US12398262B1 (en) | 2021-01-22 | 2025-08-26 | 10X Genomics, Inc. | Triblock copolymer-based cell stabilization and fixation system and methods of use thereof |
| CN117015617B (zh) | 2021-02-23 | 2025-04-04 | 10X基因组学有限公司 | 基于探针的核酸和蛋白质分析 |
| WO2022221152A1 (en) * | 2021-04-13 | 2022-10-20 | Inscripta, Inc. | Genomic edit detection at the single cell level |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8232049B2 (en) * | 2006-09-29 | 2012-07-31 | Case Western Reserve University | Method for detecting small oligonucleotides |
| GB201108259D0 (en) * | 2011-05-17 | 2011-06-29 | Cambridge Entpr Ltd | Gel beads in microfluidic droplets |
| EP3862435A1 (en) * | 2013-02-08 | 2021-08-11 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| US20150298091A1 (en) * | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| AU2015279548B2 (en) * | 2014-06-26 | 2020-02-27 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
-
2017
- 2017-04-19 US US16/094,766 patent/US20190127789A1/en not_active Abandoned
- 2017-04-19 JP JP2018554729A patent/JP2019515674A/ja active Pending
- 2017-04-19 WO PCT/US2017/028329 patent/WO2017184707A1/en not_active Ceased
- 2017-04-19 CA CA3021735A patent/CA3021735A1/en active Pending
-
2022
- 2022-10-24 US US17/972,442 patent/US20250215485A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017184707A1 (en) | 2017-10-26 |
| JP2019515674A (ja) | 2019-06-13 |
| US20250215485A1 (en) | 2025-07-03 |
| US20190127789A1 (en) | 2019-05-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220407 |
|
| EEER | Examination request |
Effective date: 20220407 |
|
| EEER | Examination request |
Effective date: 20220407 |
|
| EEER | Examination request |
Effective date: 20220407 |