JP2019509717A5 - - Google Patents
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- JP2019509717A5 JP2019509717A5 JP2018538718A JP2018538718A JP2019509717A5 JP 2019509717 A5 JP2019509717 A5 JP 2019509717A5 JP 2018538718 A JP2018538718 A JP 2018538718A JP 2018538718 A JP2018538718 A JP 2018538718A JP 2019509717 A5 JP2019509717 A5 JP 2019509717A5
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- JP
- Japan
- Prior art keywords
- residue
- amino acid
- polymerase
- position corresponding
- acid substitution
- Prior art date
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- 125000003275 alpha amino acid group Chemical group 0.000 claims 14
- 230000000875 corresponding Effects 0.000 claims 14
- 238000006467 substitution reaction Methods 0.000 claims 13
- 235000001014 amino acid Nutrition 0.000 claims 12
- 125000003372 histidine group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 claims 4
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 claims 4
- 125000000539 amino acid group Chemical group 0.000 claims 3
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims 3
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 claims 3
- 125000000012 isoleucine group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 claims 3
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 claims 3
- 229920000160 (ribonucleotides)n+m Polymers 0.000 claims 2
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 claims 2
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims 2
- 230000001915 proofreading Effects 0.000 claims 2
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 claims 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 125000000511 arginine group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 125000003616 serine group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 claims 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 1
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 claims 1
Claims (20)
- 配列番号１と少なくとも９５％同一のアミノ酸配列を含み、位置Ｙ４９３に対応する位置においてロイシン残基またはシステイン残基へのアミノ酸置換を含む、組換え古細菌ファミリーＢポリメラーゼ。
- 配列番号１に示されるアミノ酸配列の位置Ｅ６６４に対応する位置において以上のアミノ酸置換を含む、請求項１記載のポリメラーゼ。
- 配列番号１に示されるアミノ酸配列の位置Ｙ３８４、Ｖ３８９、Ｉ５２１、Ｅ６６４、またはＧ７１１に対応する位置において１つ以上のアミノ酸残基が別のアミノ酸残基に置換されている、請求項１記載のポリメラーゼ。
- 位置Ｙ４９３に対応する位置におけるロイシン残基へのアミノ酸置換を含む、請求項１記載のポリメラーゼ。
- 位置Ｙ３８４に対応する位置におけるフェニルアラニン残基、ロイシン残基、アラニン残基、システイン残基、セリン残基、ヒスチジン残基、イソロイシン残基、メチオニン残基、アスパラギン残基、またはグルタミン残基へのアミノ酸置換を含む、請求項３記載のポリメラーゼ。
- 位置Ｙ３８４に対応する位置における、ヒスチジン残基またはイソロイシン残基へのアミノ酸置換を含む、請求項５記載のポリメラーゼ。
- 位置Ｖ３８９に対応する位置における、メチオニン残基、フェニルアラニン残基、トレオニン残基、チロシン残基、グルタミン残基、アスパラギン残基、またはヒスチジン残基へのアミノ酸置換を含む、請求項３記載のポリメラーゼ。
- 位置Ｖ３８９に対応する位置における、イソロイシン残基へのアミノ酸置換を含む、請求項７記載のポリメラーゼ。
- 位置Ｉ５２１に対応する位置における、ロイシンへのアミノ酸置換を含む、請求項３記載のポリメラーゼ。
- 位置Ｅ６６４に対応する位置における、リジン残基へのアミノ酸置換を含む、請求項２記載のポリメラーゼ。
- 位置Ｇ７１１に対応する位置における、ロイシン残基、システイン残基、トレオニン残基、アルギニン残基、ヒスチジン残基、グルタミン残基、リジン残基、またはメチオニン残基へのアミノ酸置換を含む、請求項３記載のポリメラーゼ。
- 位置Ｇ７１１に対応する位置における、バリン残基へのアミノ酸置換を含む、請求項１１記載のポリメラーゼ。
- 位置Ｒ９７に対応する位置おける別のアミノ酸残基への置換を含む、請求項１記載のポリメラーゼ。
- Ａ４９０、Ｆ５８７、Ｍ１３７、Ｋ１１８、Ｔ５１４、Ｒ３８１、Ｆ３８、Ｋ４６６、Ｅ７３４、及びＮ７３５からなる群より選択される位置の１つ以上に対応する位置におけるアミノ酸置換を含む、請求項１記載のポリメラーゼ。
- プルーフリーディング活性を有する、請求項１記載のポリメラーゼ。
- プルーフリーディング活性を欠いている、請求項１記載のポリメラーゼ。
- RNAである鋳型を転写する、請求項１記載のポリメラーゼ。
- 少なくとも１０のヌクレオチドをＲＮＡテンプレートから転写する、請求項１記載のポリメラーゼ。
- 好熱性活性を有する、請求項１記載のポリメラーゼ。
- ２’−ＯメチルＤＮＡであるテンプレートを転写する、請求項１記載のポリメラーゼ。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021135313A JP7256849B2 (ja) | 2016-01-19 | 2021-08-23 | 熱安定逆転写酵素 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662280451P | 2016-01-19 | 2016-01-19 | |
| US62/280,451 | 2016-01-19 | ||
| PCT/US2017/014082 WO2017127510A2 (en) | 2016-01-19 | 2017-01-19 | Thermostable reverse transcriptase |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021135313A Division JP7256849B2 (ja) | 2016-01-19 | 2021-08-23 | 熱安定逆転写酵素 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019509717A JP2019509717A (ja) | 2019-04-11 |
| JP2019509717A5 true JP2019509717A5 (ja) | 2020-02-27 |
| JP6934477B2 JP6934477B2 (ja) | 2021-09-15 |
Family
ID=59362774
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018538718A Active JP6934477B2 (ja) | 2016-01-19 | 2017-01-19 | 熱安定逆転写酵素 |
| JP2021135313A Active JP7256849B2 (ja) | 2016-01-19 | 2021-08-23 | 熱安定逆転写酵素 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021135313A Active JP7256849B2 (ja) | 2016-01-19 | 2021-08-23 | 熱安定逆転写酵素 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US10323243B2 (ja) |
| EP (2) | EP4067491A1 (ja) |
| JP (2) | JP6934477B2 (ja) |
| WO (1) | WO2017127510A2 (ja) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017127510A2 (en) | 2016-01-19 | 2017-07-27 | Board Of Regents, The University Of Texas System | Thermostable reverse transcriptase |
| US20200216840A1 (en) * | 2017-07-27 | 2020-07-09 | Board Of Regents, The University Of Texas System | Amplification of paired protein-coding mrna sequences |
| WO2019084538A1 (en) * | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| US20220290112A1 (en) * | 2019-07-26 | 2022-09-15 | Toyobo Co., Ltd. | Variant reverse transcriptase exhibiting excellent stability |
| CN117778348B (zh) * | 2023-12-12 | 2024-09-20 | 湖北大学 | 一种具有逆转录酶活性的Pfu DNA聚合酶突变体及其应用 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030228616A1 (en) * | 1999-10-29 | 2003-12-11 | Stratagene | DNA polymerase mutants with reverse transcriptase activity |
| US20040009486A1 (en) | 1999-10-29 | 2004-01-15 | Sorge Joseph A. | Compositions and methods utilizing DNA polymerases |
| DE60141097D1 (de) | 2000-05-11 | 2010-03-11 | Toyo Boseki | Modifizierte thermostabile dna polymerase von pyrococcus kodakaraensis |
| GB0022458D0 (en) | 2000-09-13 | 2000-11-01 | Medical Res Council | Directed evolution method |
| US20030134292A1 (en) * | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
| WO2003042383A1 (fr) * | 2001-11-14 | 2003-05-22 | Toyo Boseki Kabushiki Kaisha | Promoteurs de replication de l'adn, facteurs associes a l'adn polymerase et utilisation de ces derniers |
| US7932070B2 (en) * | 2001-12-21 | 2011-04-26 | Agilent Technologies, Inc. | High fidelity DNA polymerase compositions and uses therefor |
| US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| ES2546945T3 (es) | 2003-11-03 | 2015-09-30 | Medical Research Council | Polimerasa |
| GB0325653D0 (en) | 2003-11-03 | 2003-12-10 | Medical Res Council | CST emulsions |
| US20070048748A1 (en) * | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
| ES2404665T3 (es) | 2006-09-06 | 2013-05-28 | Medical Research Council | Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante |
| DK2076592T3 (en) | 2006-10-23 | 2017-07-24 | Medical Res Council | POLYMERASE |
| US9023633B2 (en) * | 2008-11-03 | 2015-05-05 | Kapa Biosystems | Chimeric DNA polymerases |
| US20110301041A1 (en) * | 2010-02-26 | 2011-12-08 | Life Technologies Corporation | Modified Proteins and Methods of Making and Using Same |
| GB201007384D0 (en) * | 2010-04-30 | 2010-06-16 | Medical Res Council | Enzymes |
| WO2017127510A2 (en) | 2016-01-19 | 2017-07-27 | Board Of Regents, The University Of Texas System | Thermostable reverse transcriptase |
-
2017
- 2017-01-19 WO PCT/US2017/014082 patent/WO2017127510A2/en active Application Filing
- 2017-01-19 JP JP2018538718A patent/JP6934477B2/ja active Active
- 2017-01-19 EP EP22156398.4A patent/EP4067491A1/en active Pending
- 2017-01-19 US US15/410,211 patent/US10323243B2/en active Active
- 2017-01-19 EP EP17741900.9A patent/EP3405578B1/en active Active
-
2019
- 2019-05-08 US US16/407,163 patent/US10858652B2/en active Active
-
2020
- 2020-11-13 US US17/098,196 patent/US11912991B2/en active Active
-
2021
- 2021-08-23 JP JP2021135313A patent/JP7256849B2/ja active Active
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