JP2019163248A - Autonomic nerve regulator - Google Patents

Abstract

To provide an autonomic nerve regulator comprising an action for making activity of a parasympathetic nerve in a dominant state relative to activity of a sympathetic nerve, in a state in a normal time or a state in which a user feels chronic stress, and an action for sedating a central nervous system.SOLUTION: There is provided an autonomic nerve regulator comprising one or more kinds of flavor components out of flavor components derived from black tea leaves, in which the flavor components derived from the black tea leaves are preferably formed of respective flavor components which are (A) a citrus flower like flavor component, (B) a sweet flower like flavor component, (C) a mellow flower like flavor component, (D) a woody like flower component, (E) a green like flower component and (F) a cool like flavor component.EFFECT: As an effect, the autonomic nerve regulator is prepared with a food product with a performance of feeding, as an origin, the autonomic nerve regulator has high safety regardless of whether the regulator is ingested in suction or ingested in oral ingestion.SELECTED DRAWING: Figure 1

Description

  TECHNICAL FIELD The present invention relates to an autonomic nerve regulator containing an aroma component derived from black tea leaves, and more particularly to an autonomic nerve regulator comprising phototrienol and / or furaneol as an active ingredient among aroma components.

  Autonomic nerves are composed of sympathetic nerves and parasympathetic nerves, and the balance of these nerves is maintained in balance to maintain the physical and mental health. When the stress state continues for a long time, the balance of the autonomic nerves is disturbed, and the sympathetic nerves continue to be tensioned, making the parasympathetic nerves difficult to work chronically, resulting in a state in which the autonomic nerve function is dysfunctional. In a state in which the autonomic nervous function is dysfunctional, mental and physical resilience declines, and not only physical symptoms such as headaches and fatigue, but also mental symptoms such as anxiety and depression. For these symptoms, parasympathetic nerve activity is relatively superior to sympathetic nerve activity, reducing stress and sedating an increased mental state and recovering physical and mental fatigue. It is said that.

  It has been traditionally confirmed that essential oils such as lavender, jasmine, rose and narcis used in aromatherapy have a sedative effect. Moreover, the mental sedation-like fragrance | flavor composition which consists of the aromatic component which has a sedative effect contained in these essential oils is disclosed (patent documents 1-4). In addition to the effect of activating parasympathetic nerve activity, the scent of lavender has been suggested to have a sedative effect via GABA, which is a neurotransmitter that suppresses excitation in the central nervous system (Non-patent Document 1).

  Compositions containing essential oils and chemically synthesized fragrances that exhibit a sedative effect are compositions that are produced through extraction, purification, and chemical synthesis using an organic solvent, and contain components that have a sedative effect in high purity. Products can be made, but due to the characteristics of the fragrance and food safety issues, the use is mainly limited to cosmetics. There is a demand for a fragrance composition that is highly safe and has a sedative effect that can be applied as a food, and the mental sedative function of a fragrance component contained in a palatability beverage with a proven track record has attracted attention. Specifically, research has been conducted on the relaxation effect due to the aroma of jasmine tea, beer and fermented milk, and the stress reduction effect due to the aroma of whiskey, chamomile tea and coffee.

  Especially, teas such as green tea, oolong tea and black tea made from tea tree (Camellia sinensis) are the most popular drinks in the world, and the ingredients contained in tea have psychological and physiological effects. That is widely recognized. It has been reported that theanine, which is an amino acid component unique to tea contained in a large amount of green tea, has an anti-stress action that promotes the release of dopamine in the brain and soothes excitatory stimuli (Non-patent Document 2). Moreover, the autonomic-nerve adjustment effect | action (patent document 5) by ingesting the tea extract containing theaflavins orally is disclosed. On the other hand, tea contains caffeine that has pharmacological effects such as central nervous excitement and arousal, so drinking a large amount of tea leads to excessive intake of caffeine, and excitement such as insomnia, anxiety, and nervousness. It has been regarded as a problem that this occurs (Non Patent Literature 3).

  It is said that there are more than 600 types of fragrance components contained in teas, and the difference in the type and production method of raw material leaves such as varieties and production areas greatly affects the composition of the fragrance components, resulting in differences in fragrance characteristics. In particular, black tea with an oxidative fermentation process in the production process has a new fragrance component produced during processing, and the fragrance becomes more complicated (Non-Patent Document 1).

  An anti-stress agent (Patent Document 6) containing an aroma component of coffee or tea as an active ingredient is disclosed as a composition exhibiting an anti-stress effect using an aroma component of tea. Patent Document 6 discloses 2-methyl-3-buten-2-ol and 3-methyl-2-butene- which are aromatic components contained in coffee or tea. 1-ol (3-methyl-2-buten-1-ol), 1-penten-3-ol (1-penten-3-ol), trans-2-hexen-1-ol (trans-hexen-1 -ol), 1-octen-3-ol, 1-octen-3-ol, sotolone, 2,4-dimethylstyrene, benzothiazole and 2,3, 5-Trimethylphenol (2,3,5-trimethylphenol) has been shown to have a receptor activating effect on GABA. In addition, cis-jasmone, methyl jasmonate, jasmin lactone, and linalool oxide, which are characteristic flavors of semi-fermented tea, have similar effects. It has been shown.

  Moreover, it has been reported that the increase in chromogranin A in saliva accompanying acute stress load is suppressed by inhalation of darjeeling black tea (Non-Patent Document 4). Chromogranin A in saliva is a biomarker that measures the activity of sympathetic nerve activity among endocrine reactions associated with stress load (Non-patent Document 5). Therefore, the fragrance of Darjeeling black tea has the effect of suppressing the activation of sympathetic nerve activity due to temporary stress, but it improves the state of chronic sympathetic nerve predominance and relatively predominates parasympathetic nerve activity. It is unclear whether it has a calming effect.

JP-A-3-111493 JP-A-5-230495 JP-A-5-202380 JP-A-6-25693 JP 2017-109997 A WO2005-011718

Bulletin of Kansai Medical University (2012) vol6, pp109-115 The health function and culture of black tea, edited by Mitsuaki Sano / Yumi Saito (I-K Corporation) “Health Food” Safety and Efficacy Information-Health Food Ingredient Information Database Cha (tea) Updated: December 15, 2017 (National Institute of Health and Nutrition) Journal of Physiological Anthropology (2018) 37: 3 Kochi Women's University Journal of Nursing (2014) Vol.40, No.1, pp24-30

  An object of the present invention is to provide an autonomic nerve modulating agent capable of showing an effect of predominating parasympathetic nerve activity in the autonomic nervous system for a person who is in a state of feeling chronic stress, thereby reducing the stress. There is to make it.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that the aroma component contained in black tea leaves exhibits a high autonomic nerve regulating action and predominates parasympathetic nerve activity. It has been found that the aroma component of the component composition has the effect of sedating the central nervous system and controlling the autonomic nerves to make parasympathetic activity predominate. Among them, phototrienol and / or furaneol has an extremely high autonomic nerve modulating action. It has been shown that the parasympathetic nerve activity is superior, and the present invention has been completed.

That is, this invention is as follows specified by the following matters.
[1] An autonomic nervous regulator comprising one or more fragrance components derived from black tea leaves.
[2] Aroma components derived from tea leaves are (A) citrus flowers, (B) sweet flowers, (C) mellow flowers, (D) woody, (E) green and (F) cool The autonomic nerve regulating agent according to [1], which is composed of each aroma component.
[3] (A) Citrus flowers, (B) Sweet flowers, (C) Rich flowers, (D) Woody, (E) Green and (F) Cool The autonomic nerve regulating agent according to [2], which comprises an aroma component shown.
(A) linalool oxide (trans-franoid type) (linalool oxide (trans-franoid)), linalool oxide (cis-furanoid type) (linalool oxide (cis-franoid)), linalool (hotalenol), α-terpineol (Α-terpineol), linalool oxide (cis-pyranoid type) (linalool oxide (cis-pyranoid)), citral, linalool oxide (trans-pyranoid type) (linalool oxide (trans-pyranoid)), nerol (nerol) or Geraniol
(B) benzaldehyde, phenylacetaldehyde, β-damascenone, benzylalcohol, 2-phenylethylalcohol or cis-jasmone
(C) 6-methyl-5-hepten-2-one, β-cyclocitral, α-ionone, β-ionone (Β-ionone) or 5,6-epoxy-β-ionone (5,6-epoxy-β-ionone)
(D) β-myrcene, α-terpinene, D-limonene, α-phellandrene, trans-β-ocimene Γ-terpinene, cis-β-ocimene, p-cymene or terpinolene
(E) hexanal, trans-2-hexen-1-al, cis-3-hexen-1-ol, acetate, hexanol (Hexanol), cis-3-hexen-1-ol (cis-3-hexen-1-ol), trans-2-hexen-1-ol (trans-2-hexen-1-ol) or trans-2 butyric acid -Hexenyl (trans-3-hexenyl butyrate)
(F) Methyl salicylate
[4] The peak area ratio of each aromatic component measured by gas chromatography mass spectrometry (GC / MS analysis) is {(A) + (D)} / {(A) + (B) + (C) + (D) + (E) + (F)} ≧ 0.53
The autonomic nerve regulating agent according to [2] or [3], which satisfies the following condition:
[5] The fragrance component contains 0.01 ppm or more of phototrienol, and the peak area ratio measured by gas chromatography mass spectrometry (GC / MS analysis) of each fragrance component is
{(B) + (C)} / {(A) + (B) + (C) + (D) + (E) + (F)} ≦ 0.28
Or
{(E) + (F)} / {(A) + (B) + (C) + (D) + (E) + (F)} ≦ 0.29
The autonomic nerve regulating agent according to [2] or [3], which satisfies the following condition:
[6] The autonomic nervous regulator according to any one of [1] to [5], containing 0.1 to 10 ppm of phototrienol.
[7] The autonomic nerve regulating agent according to any one of [2] to [6], further comprising (G) a sweet-like component as an aroma component.
[8] The autonomic nerve regulating agent according to [7], wherein the (G) sweet-like component is furaneol.
[9] The autonomic nervous regulator according to [8], containing 0.007 to 1 ppm of furaneol.
[10] The autonomic nervous regulator according to any one of [3] to [9], which contains 0.2 to 4.8 ppm of methyl salicylate.
[11] The autonomic nerve regulating agent according to any one of [3] to [10], which contains 0.1 to 12.7 ppm of linalool.
[12] The autonomic nerve modulating agent according to any one of [1] to [11], which is an autonomic nerve regulating action by sedation of the central nervous system.
[13] An autonomic neuromodulator comprising phototrienol and / or furaneol as an active ingredient.
[14] The autonomic nervous regulator according to [13], containing 0.05 to 100 ppm of phototrienol and / or 0.02 to 10 ppm of furaneol.
[15] The autonomic nerve control according to [13] or [14], containing phototrienol and furaneol, containing 0.05 ppm or more of phototrienol, and the total of phototrienol and furaneol being 0.1 ppm or more Agent.
[16] A drug for regulating autonomic nerves, a functional food, a supplement, a food or drink, or a cosmetic containing the autonomic nerve modulating agent according to any one of [1] to [15].
[17] A method for reducing, alleviating, preventing or recovering stress symptoms, characterized by causing a human to ingest the autonomic nervous regulator according to any one of [1] to [15] from the skin, nasal or oral Or mental sedation method.

  The autonomic nervous modulator of the present invention can make parasympathetic nerve activity relatively dominant in a person who is chronically stressed. As a result, it is possible to improve, prevent and / or treat various physical symptoms resulting from the continued state of increased sympathetic nerve activity. Further, by calming down the central nervous system, it is possible to reduce, alleviate or improve the psychological burden caused by physical or mental stress.

The score using the visual evaluation scale (VAS) with respect to each item shown on the horizontal axis before and after inhalation of darjeeling aroma in Test Example 1 is shown. The score using the multifaceted emotional state scale reduced version (MMS) with respect to each item shown on the horizontal axis before and after inhalation of darjeeling fragrance in Test Example 1 is shown. The amount of change of the miosis rate using the measurement method of the pupillary light reaction before and after the inhalation of darjeeling aroma and hot water (control) in Test Example 1 is shown. The skin temperature change amount by the skin temperature measurement of the peripheral site | part before and behind inhalation of darjeeling fragrance and hot water (control) in Test Example 1 is shown. The amount change of oxygenated hemoglobin (Oxy-Hb) concentration in the prefrontal cortex using near infrared spectroscopy (NIRS) before and after inhalation of Darjeeling aroma and Sayu (control) in Test Example 1 is shown. The score change amount using VAS with respect to each item shown on the horizontal axis before and after inhalation of the aroma of black tea according to production area in Test Example 2 is shown. The pupillary ratio change amount using the pupillary photoreaction measurement method before and after inhalation of the aroma of black tea by production area in Test Example 2 is shown. The skin temperature variation | change_quantity by the skin temperature measurement of the peripheral site | part before and behind inhalation of the fragrance | flavor of the black tea according to production area in Experiment 2 is shown. The preference of the aroma of tea by production area in Test Example 2 is shown.

Hereinafter, the present invention will be described in detail.
The autonomic nervous regulator of the present invention is characterized in that it uses tea leaves and stems that have undergone an oxidative fermentation process of canola (Camellia sinensis) as raw materials, and contains one or more aroma components contained therein. Among these fragrance components, the fragrance component having an autonomic nerve regulating action is preferable, and the autonomic nerve regulator of the present invention contains one or more kinds of aroma components derived from tea leaves having an autonomic nerve regulating action. Is preferred.
In the present invention, “autonomic nerve regulating action” means that the parasympathetic nerve activity is relatively superior to the sympathetic nerve activity in a person who is chronically stressed, and the mood such as fatigue, stress, boredom, motivation, etc.・ Reducing emotions and raising mood and emotions such as a refreshing feeling, concentration, and exhilaration (i.e., relieving depression, anxiety, and hostility, strengthening feelings of affinity and cooperation, and providing a sense of relaxation and exhilaration Increase).

  In addition, “sedation of central nervous system” means calming brain functions related to emotional control, reducing feelings and feelings such as fatigue, spontaneous stress, depression, anxiety and hostility, and exhilaration and inactivity. It means an action that raises the mood and emotions of the target and brings about emotional changes. In general, the sedating effect of the central nervous system refers to the effect of relaxing. Emotion means transient and sudden emotional movements that are caused relatively quickly, such as anger, fear, joy, and sadness. Autonomic nerve activity, which is one of physiological responses caused by emotional changes, can be regulated by the aroma composition of the present invention.

  The autonomic nervous regulator of the present invention can be prepared from tea leaves and stem leaves that have been subjected to the oxidative fermentation process of canola (Camellia sinsnsis) using a known method. Examples of the tea leaves that can be used as the raw material of the present invention include Darjeeling, Assam, Nilgiri, Kenya, Kemon, Rhapsang Soo Chon, Nuwara Area, Uba, Dimbra, etc. Among them, 2nd flush of Darjeeling (second flush) is autonomous. It is more preferable because it contains an effective aroma component exhibiting a neuromodulating action at a high concentration. In addition, it is also possible to use generally distributed teas as a raw material.

  Examples of the method for producing an aroma component used in the present invention include a method for preparing tea from an extraction process such as vacuum distillation, steam distillation, and adsorption / desorption on an adsorbent. For example, after adding hot water or warm water to black tea to obtain an extract, the steam component generated in the distillation step by heating under normal pressure or reduced pressure is recovered to obtain an aroma component contained in black tea Can do.

Moreover, the aromatic component used for this invention can also be manufactured by the steam distillation method. The steam distillation method is a method in which steam is blown into an aqueous solution containing tea leaves or an extract from tea leaves, and aroma components volatilized with the steam are cooled and liquefied and recovered as a distillate. Examples thereof include steam distillation and vacuum steam distillation. As the water vapor, either saturated water vapor or pressurized water vapor may be used. Any of the temperature and flow rate of water vapor to be injected, the cooling temperature of water vapor, the amount of distillate, etc. may be used. The temperature and flow rate of the steam to be injected, the cooling temperature of the steam, the amount of distillate, etc. can be arbitrarily set according to the type of raw tea leaves. The steam temperature is 40 to 110 ° C. and the flow rate is 0 per kg of raw tea leaves. 2 to 20 kg / hr, the cooling temperature is −10 to 70 ° C., and the distillate amount is 0.5 to 2.5 kg per 1 kg of tea leaves, but is not limited to this range.
Examples of the method for producing an aroma component in other inventions include a supercritical extraction method using supercritical water or supercritical carbon dioxide from tea leaves.

The obtained aromatic component can be obtained as a higher concentration aromatic component by further rectifying under normal pressure or reduced pressure. Furthermore, it can concentrate by non-heating concentration methods, such as a reverse osmosis membrane, and can preserve | save frozen by reducing the oxygen concentration in air | atmosphere by nitrogen substitution etc. and storing it frozen.
Moreover, the obtained aromatic component may be purified from the tea leaf extract using a known method such as column chromatography, or may be a non-purified concentrate. In addition, the aromatic component may be a single compound alone or a mixture of a plurality of compounds among the compounds contained in the black tea extract.
The aroma component thus obtained can be used as the autonomic nervous regulator of the present invention by formulating it as it is or appropriately.

  In the present invention, the meaning of “derived from black tea leaves” means “contained in black tea leaves”, and therefore “the aromatic components derived from black tea leaves” is an aromatic component contained in black tea leaves, It may be extracted according to the above method, may be a mixture of commercial products that have already been purified, or may be chemically synthesized, and has a desired aroma composition. It is also possible to adjust the concentration by adding a commercially available or chemically synthesized aroma component to one extracted from black tea leaves. Such adjustment may be outsourced to a specialized manufacturer.

  The autonomic nervous regulator of the present invention contains one or more fragrance components derived from the above-obtained tea leaves. Examples of the fragrance components include green-like, woody-like, dry-like, flower-like, Fruit-like, sweet-like, roast-like, spicy-like or cool-like fragrance components can be mentioned, but the fragrance component is composed of flower-like, woody-like, green-like, and cool-like odors, and has an autonomic nervous control action. It can mention preferably in the point which has. Furthermore, as a flower-like aroma component, the flower-like aroma component comprised from a citrus flower-like, sweet flower-like component, and a mellow flower-like aroma component is mentioned preferably, for example.

  (A) As a citrus flower-like aroma component, for example, linalool oxide (trans furanoid type), linalool oxide (cis furanoid type), linalool, phototrienol, α-terpineol, linalool oxide (cis pyranoid type), citral, linalool oxide (trans Pyranoid type), nerol, geraniol, nerolidol, citronellol, rose oxide, etc., among which linalool oxide (trans furanoid type), linalool oxide (cis furanoid type), linalool, phototrienol, α-terpineol, linalool oxide (cis) Pyranoid type), citral, linalool oxide (trans pyranoid type), nerol or geraniol are preferably used, and these are used alone or in combination of two or more. Door can be.

  (B) Examples of the sweet flower-like aroma component include benzaldehyde, phenylacetaldehyde, β-damasenone, benzyl alcohol, 2-phenylethyl alcohol, cis-jasmon, benzyl acetate, benzyl benzoate, and the like. Among them, benzaldehyde, phenyl Acetaldehyde, β-damasenone, benzyl alcohol, 2-phenylethyl alcohol or cis-jasmon are preferably mentioned, and these can be used alone or in combination of two or more.

  (C) As a mellow flower-like aroma component, for example, 6-methyl-5-hepten-2-one, β-cyclocitral, α-ionone, β-ionone and 5,6-epoxy-β-yonone, indole, iso Eugenol and the like can be mentioned, among which 6-methyl-5-hepten-2-one, β-cyclocitral, α-ionone, β-ionone or 5,6-epoxy-β-yonone are preferred, and these are 1 One species can be used alone, or two or more species can be mixed and used.

  (D) Woody-like aroma components include, for example, β-myrcene, α-terpinene, γ-terpinene, D-limonene, α-ferrandrene, cis-β-osimene, trans-β-osymene, p-cymene, terpinolene, Examples include tyuopsen, kadinene, and casinomal, among which β-myrcene, α-terpinene, γ-terpinene, D-limonene, α-ferrandlene, cis-β-osimene, trans-β-osimene, p-cymene or terpinolene. Are preferable, and these can be used alone or in combination of two or more.

  (E) As a green-like aroma component, for example, hexanal, trans-2-hexen-1-al, cis-3-hexenyl acetate, hexanol, cis-3-hexen-1-ol, trans-2-hexene-1- Ol, butyric acid trans-2-hexenyl, hexanoic acid, trans-2-hexenoic acid, trans-2-heptenal, 1-penten-3-ol, etc., among which hexanal, trans-2-hexen-1-al, Preferred examples include cis-3-hexenyl acetate, hexanol, cis-3-hexen-1-ol, trans-2-hexen-1-ol, and trans-2-hexenyl butyrate. These may be used alone or in combination of two or more. Can be mixed and used.

  (F) Cool-like aroma components include, for example, methyl salicylate, carvone, mentholfuran, menthol, menthone, menthyl acetate, pregon, rotundiphorone, camphene, camphor, etc. Among them, methyl salicylate is preferred, and these are 1 One species can be used alone, or two or more species can be mixed and used.

  (G) Examples of the sweet-like fragrance component include furaneol, cycloten, maltol, and isomaltol. Among these, furaneol is preferred, and these may be used alone or in combination of two or more. it can.

  Examples of the aromatic component used in the present invention include one or a combination of two or more selected from the group consisting of the aromatic components (A) to (G). Among them, linalool, geraniol, 2-phenylethyl One or a combination of two or more selected from the group consisting of alcohol, methyl salicylate, trans-2-hexen-1-al and cis-3-hexen-1-ol can be preferably exemplified, and phototrienol and / or Or it is preferable to contain furaneol.

  In addition, when the composition ratio of the fragrance component is analyzed by gas chromatography mass spectrometry (GC / MS analysis method), the sum of the peak area values of the fragrance components (A) to (F) is ( It is preferable that the total value of peak area values of A) citrus flower-like and (D) woody-like fragrance components is 53% or more.

Moreover, it contains 10 ppb or more of phototrienol, and (B) sweet flower-like and (C) mellow flower-like aroma component peak area values with respect to the sum of peak area values of aroma components (A) to (F) The total value is preferably 28% or less.
Or the total value of the peak area value of (E) green-like and (F) cool-like fragrance component with respect to the sum total of the peak area value of the fragrance component of (A)-(F) containing 10 ppb or more of phototrienol. Is preferably 29% or less.

  The content of phototrienol is not particularly limited, but is preferably 10 ppb or more, and more preferably in the range of 0.1 to 10 ppm.

  The fragrance component used in the present invention preferably further includes (G) a sweet-like fragrance component. (G) Examples of the sweet-like aroma component include furaneol, cycloten, maltol, and isomaltol. Among these, furaneol is preferable, and these can be used alone or in combination of two or more. . Among these, furaneol can be preferably exemplified.

Although the content concentration of furaneol is not particularly limited, a range of 0.007 to 1 ppm can be preferably exemplified.
Further, the content concentration of methyl salicylate contained as the (F) cool-like component is not particularly limited, but it is preferably contained in the range of 0.2 to 4.8 ppm.
Moreover, the content concentration of linalool contained as the citrus flower-like aroma component (A) is not particularly limited, but it is preferably contained within the range of 0.1 to 12.7 ppm.

Examples of the autonomic nerve modulating agent according to another aspect of the present invention include those containing fotrienol and / or furaneol as an active ingredient among the aroma components.
The content concentration of phototrienol and furaneol is not particularly limited, but phototrienol is preferably in the range of 0.05 to 100 ppm, and furaneol is preferably in the range of 0.02 to 10 ppm.
Furthermore, it is preferable to contain a phototrienol and furaneol, and the content concentration is preferably 0.05 ppm or more for phototrienol, and the total content concentration of phototrienol and furaneol is preferably 0.1 ppm or more.

  When the aroma component derived from black tea contained in the autonomic nervous regulator of the present invention is a free acid or base, the aroma component may be contained in the form of a pharmaceutically acceptable salt. Examples of the pharmaceutically acceptable salt include salts with pharmaceutically acceptable acids (for example, inorganic acids or organic acids) and bases (for example, inorganic bases or organic bases). More specifically, for example, inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid, hydrobromic acid; organic acids such as oxalic acid, maleic acid, fumaric acid, malic acid, tartaric acid, citric acid, benzoic acid; sodium or Examples thereof include inorganic bases such as alkali metals such as potassium, alkaline earth metals such as magnesium and calcium; and salts with organic bases such as organic amines such as triethylamine and basic amino acids such as arginine.

  The form of use of the autonomic nervous modulator of the present invention is not limited, and examples thereof include liquid, powder, and granule. In the case of foods or nutritional supplements, liquids, suspensions, powders, granules, fine granules, tablets, capsules, syrups and the like are usually used.

  The autonomic nervous regulator of the present invention can be used as a pharmaceutical or a functional food. When the autonomic nervous modulator of the present invention is used as a pharmaceutical product, the pharmaceutical product may be in any dosage form as long as it is conveniently administered orally or parenterally. Examples of pharmaceutical preparation forms include tablets, granules, fine granules, pills, powders, capsules, troches, chewables, liquids (drinks), infusions, and the like. In the case of an external preparation, any form may be used as long as it is absorbed into the body from the skin surface or mucous membrane, for example, lotion, ointment, cream, gel, tape, patch, aerosol, Inhalants and the like can be mentioned, and the pharmaceutical products according to the present invention in the above dosage forms can be used alone or in combination depending on the symptoms.

In the pharmaceutical product according to the present invention, known additives that can be usually used in the pharmaceutical formulation technical field such as excipients, binders, disintegrants, lubricants, taste-masking agents, stabilizers, etc., as main agents in accordance with the purpose. Can be added.
More specifically, when the pharmaceutical product of the present invention is a solid preparation such as a capsule, tablet, powder or granule, for example, excipients such as lactose, glucose, sucrose, mannitol; starch, alginic acid Disintegrants such as soda; lubricants such as magnesium stearate and talc; binders such as polyvinyl alcohol, hydroxypropyl cellulose and gelatin; surfactants such as fatty acid esters; additives such as plasticizers such as glycerin It can be contained. When the pharmaceutical product according to the present invention is a liquid preparation, for example, sugars such as sucrose, sorbit and fructose; glycols such as polyethylene glycol and propylene glycol; oils such as sesame oil, olive oil and soybean oil; p- Additives such as preservatives such as hydroxybenzoates can be included in the formulation. In the case of a liquid formulation, the pharmaceutical product according to the present invention may be a formulation in which an aromatic component as an active ingredient is dissolved at the time of use.
The pharmaceuticals according to the present invention as described above can be easily produced according to methods well known or commonly used in the field of pharmaceutics.

The administration route of the pharmaceutical agent according to the present invention is not particularly limited, and may be either oral administration or parenteral administration. Among these, it is preferable from the viewpoint of ease of taking that the medicament according to the present invention can be orally, transdermally, transdermally absorbed, nasally or inhaled.
Moreover, since the dosage of the pharmaceutical agent according to the present invention varies depending on the administration route, the dosage form, the severity of the symptom of the patient, the age or the body weight, it cannot be generally stated. Specifically, a single dose for an adult is about 1 to 500 mg, preferably about 5 to 50 mg as the total amount of the aroma components.

  When the autonomic nervous regulator of the present invention is used as a functional food, the form may be the same as that of the aforementioned pharmaceutical preparation. Examples of the functional food according to the present invention include confectionery such as biscuits, cookies, cakes, candy, chocolate, chewing gum and Japanese confectionery; bread, noodles, rice, tofu or processed products thereof; fermented foods such as sake and medicinal liquor; Seasonings such as mirin, vinegar, soy sauce, miso, dressing; livestock foods such as yogurt, ham, bacon, sausage, mayonnaise; marine foods such as kamaboko, fried tempura, hampen; fruit juice drinks, soft drinks, sports drinks, alcohol Examples include beverages such as beverages, tea, coffee, and cocoa. When the autonomic nervous modulator of the present invention is used by adding it to beverages, the amount of aroma components contained is preferably 0.0005 to 1.0% by mass per liter of beverage, and the intake per serving is included. The total amount of aroma components to be added is about 1-50 mg, preferably about 1-5 mg.

  The functional food according to the present invention may contain auxiliary ingredients commonly used in the food field. Examples of the auxiliary component include lactose, sucrose, liquid sugar, honey, magnesium stearate, oxypropylcellulose, various vitamins, trace elements, citric acid, malic acid, fragrance, and inorganic salt.

  The autonomic nervous regulator of the present invention may be taken when a psychological load due to physical or mental stress is expected, for example, during physical work, mental work, or sports. Moreover, you may use regularly in order to prevent or reduce stress. In such a case, the amount of intake varies depending on the condition, age, sex, etc. of the person who takes it, so it cannot be generally stated. Specifically, a single intake for an adult is about 5 mg to 500 mg, preferably about 5 mg to 50 mg, as the total amount of aroma components contained.

  The autonomic nervous regulator of the present invention can be used as a cosmetic or a fragrance. For example, perfume, eau de toilette, colon, antiperspirant, deodorant, shampoo, rinse, hair conditioner, hair treatment, hair grooming, soap, body soap, basic cosmetics and other human products, clothing detergent, residential cleaner, medical Softeners for clothes, bleach for clothing, kitchen dish cleaner, toilet cleaner, residential bleach, fragrance, floor polish, toothpaste, mouthwash, mouth deodorant, mouth freshener, etc. It can be used for miscellaneous products such as patches, tissue paper, toilet paper, masks, etc.

Cosmetics containing the autonomic nervous regulator of the present invention can use any cosmetic ingredients blended in the cosmetic field such as oils and fats, moisturizers, natural pigments, surfactants, antioxidants, preservatives, It can be produced by a method known per se. By using the cosmetics of the present invention, it is freed from physiological psychological states such as drowsiness, fatigue, and drowsiness that humans experience in daily life, and has the effect of refreshing mood and activating mental activity. Is. The blending amount of the fragrance component in the cosmetic is appropriately determined depending on the form of the cosmetic and the purpose of use, but is generally 0.005 to 10% by mass with respect to the entire cosmetic.
The fragrance containing the autonomic nerve regulating agent of the present invention can be used by being stored in a container known per se. Examples of the shape of the fragrance include spray and solid (granular), and a fragrant space can be created. Moreover, the use method which volatilizes in air using a diffuser (diffuser) and a user is exposed to this air for a fixed time can also be illustrated. The exposure time is not particularly limited, but about 10 minutes to 1 hour is appropriate. The amount of the fragrance component in the fragrance is appropriately determined depending on the place of use and the required fragrance strength, but is generally 0.005 to 10% by mass with respect to the entire fragrance. is there. If the concentration is volatilized in the air by these methods, if it is too low, the desired effect cannot be obtained, and if it is too high, fine particles condensed in the air may be precipitated, so it is preferably 0.01-100 ppb. Volatilize as follows.

  Examples The present invention will be described more specifically with reference to examples. However, the present invention is not limited to the examples.

<< Aroma component analysis and quantification method by GC-MS >>
As a sample, 2.5 g of tea leaves were extracted with 150 mL of hot water and cooled to room temperature. 10 mL of a sample diluted 10-fold with ultrapure water and 3 g of sodium chloride are placed in a 20 mL SPME vial and cycloheptanol (manufactured by Tokyo Chemical Industry Co., Ltd.) is added as an internal standard substance to a final concentration of 500 ppb. Aroma water. When performing aroma component analysis in aroma water, the aroma water was appropriately diluted with ultrapure water. The aroma component was recovered by solid phase micro extraction (SPME) and subjected to gas chromatograph mass spectrometry (GC-MS analysis). The GC-MS analysis conditions are as follows. The ratio of the peak area of each fragrance component to the internal standard was taken as the peak area value.
The quantification was carried out using linalool (linalool: manufactured by Tokyo Chemical Industry Co., Ltd.), phototrienol (hotorienol: synthesized by a known synthesis method), methyl salicylate (methyl salicylate: manufactured by Tokyo Chemical Industry Co., Ltd.), trans-2-hexene as standard substances. -1-R (trans-2-hexen-1-al: manufactured by Tokyo Chemical Industry Co., Ltd.), cis-3-hexen-1-ol (cis-3-hexen-1-ol: manufactured by Tokyo Chemical Industry Co., Ltd.), geraniol (Geraniol: manufactured by Tokyo Chemical Industry Co., Ltd.) and 2-phenylethyl alcohol (2-phenylethylalcohol: manufactured by Tokyo Chemical Industry Co., Ltd.) were dissolved in ultrapure water, diluted stepwise and used as a standard solution. 10 mL of each standard solution and 3 g of sodium chloride were placed in a 20 mL SPME vial, and an analysis sample was prepared by adding cycloheptanol (manufactured by Tokyo Chemical Industry Co., Ltd.) as an internal standard substance to a final concentration of 500 ppb. Aroma components are recovered by solid phase microextraction and subjected to GC-MS analysis, and linalool, phototrienol, methyl salicylate, trans-2-hexen-1-al, cis-3-hexen-1-ol, geraniol and 2- Phenylethyl alcohol was quantified.

<SPME-GC / MS conditions>
GC: TRACE GC ULTRA (Thermo Fisher Scientific)
MS: TSQ QUANTUM XLS (Thermo Fisher Scientific)
SPME fiber: 50 / 30μm Divinylbenzene / Carboxen / Polydimethylsiloxane Stableflex
Extraction: 60 ° C., 30 minutes Column: SUPELCO WAX10 (0.25 mm ID × 60 m × 0.25 μm, Sigma-Aldrich)
Oven program: Hold at 40 ° C. for 2 minutes, then heat up to 160 ° C. at 3 ° C./minute, then heat up to 280 ° C. at 10 ° C./minute Carrier gas: Helium (100 kPa, constant pressure)
Injector temperature: Splitless, 240 ° C
Ion source temperature: 200 ° C
Ionization: Electron ionization Ionization voltage: 70 eV
Measurement mode: Scan

Monitoring ions: hexanal; m / z = 56, trans-2-hexen-1-al; m / z = 69, cis-3-hexenyl acetate ( cis-3-hexen-1-ol-acetate); m / z = 67, hexanol; m / z = 56, cis-3-hexen-1-ol (cis-3-hexen-1-ol) M / z = 67, trans-2-hexen-1-ol; m / z = 57, trans-3-hexenyl butyrate; m / Z = 67, β-myrcene; m / z = 93, α-terpinene; m / z = 121, γ-terpinene; m / z = 93, D-limonene; m / z = 68, α-phellandrene; m / z = 93, cis-β-ocimene; m / z = 93, trans-β-o M / z = 93, p-cymene; m / z = 93, terpinolene; m / z = 119), linalool; m / z = 93, linalool oxide (cis-franoid); m / z = 93, linalool oxide (trans-franoid); m / z = 93, linalool Oxide (cis pyranoid type) (linalool oxide (cis-pyranoid)); m / z = 94, linalool oxide (trans-pyranoid type) (linalool oxide (trans-pyranoid)); m / z = 94, hotrienol; m / z = 71, citral; m / z = 94, nerol; m / z = 69, geraniol; m / z = 69, α-terpineol; m / z z = 93, benzaldehyde (Benzaldehyde); m / z = 109, phenylacetaldehyde; m / z = 91, β-damascenone; m / z = 121, benzylalcohol; m / z = 91, 2 2-phenylethylalcohol; m / z = 91, cis-jasmone; m / z = 122, 6-methyl-5-hepten -2-one); m / z = 108, β-cyclocitral; m / z = 152, α-ionone; m / z = 121, β-ionone M / z = 177, 5,6-epoxy-β-ionone; m / z = 123, methyl salicylate; m / z = 120, cyclohepta Nord; m / z = 57

<< Method of quantifying furaneol by LC-MS >>
As a sample, 2.5 g of tea leaves were extracted with 150 mL of hot water and cooled to room temperature. A sample filtered through a 0.45 μm filter (Advantech) was used for liquid chromatography mass spectrometry (LC-MS analysis). LC-MS analysis conditions are as follows. For the quantification, furaneol (manufactured by Tokyo Chemical Industry Co., Ltd.) as a standard substance was dissolved in ultrapure water and diluted stepwise to obtain a standard solution.

<LC conditions>
Equipment used: Agilent 1100 Series (Agilent technologies)
Column: Atlantis T3 2.1 × 100mm, particle size 3μm
Column temperature: 40 ° C
Flow rate: 0.2 mL / min
Mobile phase: (A) Water + 0.1% (v / v) formic acid (B) Acetonitrile + 0.1% (v / v) Formic acid Gradient conditions: 0 min (A) 95% (B) 5%, 1 min ( A) 95% (B) 5%, 21 minutes (A) 20% (B) 80%, 24 minutes (A) 0% (B) 100%, 30 minutes (A) 95% (B) 5%

<MS conditions>
Equipment used: 3200QTRAP (AB SCIEX)
Spray voltage: 5500V
Ion source temperature: 600 ° C
Curtain gas: 10 psi
Collision gas: 3 psi
Measurement mode: Scan, Multiple Reaction Monitoring (MRM)
MRM conditions: Q1 (m / z) = 129.04, Q2 (m / z) = 100.90

《Method for evaluating psychological state》
(1) For evaluation of mood, a questionnaire method based on a visual evaluation scale (Visual Analogue Scale (VAS)) was used to investigate the mood state. Seven items were used: “Overall fatigue”, “Spontaneous stress”, “Boring”, “Neat head”, “Concentration”, “Motivation”, “Exhilaration”. Subjective evaluation was determined with a line scale of 0 mm; “not felt at all” to 100 mm at maximum; “I felt the strongest so far”. The length from the 0 value to the checked mark was measured, and those values were used as the mood index (VAS score). According to a report by Collins SL et al. (Pain. 72 (1-2): 95-7, AUG 1997), when pain intensity is evaluated by VAS, at least moderate pain is shown when the VAS score exceeds 30 mm. It has been shown. From this, when the VAS score exceeded 30 mm, it was determined that a moderate or higher stress was felt. In addition, the statistical analysis of each measurement result used the t-test or the Bonferroni multiple comparison test after one-way analysis of variance, and the significance level was set to less than 5%.
(2) For the evaluation of emotions, the state of emotions was investigated by using a questionnaire method based on a multiple mood scale (MMS). We used 40 sub-scale 40 items of “depression / anxiety”, “hostility”, “fatigue”, “active”, “inactive”, “affinity”, “concentration”, “amazing”. Subjective evaluation was sought with a four-level rating from 1; “I don't feel at all” to 4; 1 to 4 were simply added, and the total score of each item was used as an emotion index (MMS score). In addition, the statistical analysis of each measurement result used the t-test or the Bonferroni multiple comparison test after one-way analysis of variance, and the significance level was set to less than 5%.

<Method for evaluating autonomic nervous activity>
(1) The pupillary photoresponse measurement method was used for evaluation of the state of the autonomic nerve, particularly the parasympathetic nerve activity. A goggle-like electronic pupillometer was attached to the face, and after dark adaptation for 2 minutes in a light-shielded state, light stimulation was performed for 0.1 seconds. The pupil diameter D1 in the initial state before light stimulation and the change amount D1-D2 of the pupil diameter D2 after light stimulation were used, and the miosis ratio (CR), which is the ratio of miosis, was obtained by (D1-D2) / D1. The pupillary pupil ratio in the blank was CR1, the pupil pupil ratio in the control was CR2, and the pupil ratio in the sample was CR3. The pupillary ratio change amount ΔCR = CR2—CR1 in the control and the pupillary ratio change amount ΔCR = CR3—CR1 in the sample. The case where the amount of change in the miosis rate was less than 0.04 was determined to be “with weak action”, the case with 0.04 or more was determined to be “with action”, and the case with 0.05 or more was determined to be “with sufficient action”. In addition, the statistical analysis of each measurement result used the t-test and the Bonferroni multiple comparison test after one-way analysis of variance, and the significance level was set to less than 5%.
(2) The skin temperature measurement method of the peripheral part was used for the evaluation with respect to the state of an autonomic nerve, especially a sympathetic nerve activity. Changes in the skin temperature (central forehead and central index finger) at the peripheral site were continuously measured with a temperature sensor. The skin temperature at the center of the forehead in the blank is H1, the skin temperature at the center of the forefinger is F1, the center of the forehead at the control is H2, the center of the forefinger is F2, and the center of the forehead in the sample is H3. Was F3. Skin temperature change amount (° C.) ΔT = (F 2 −F 1) − (H 2 −H 1) in the control and skin temperature change amount (° C.) ΔT = (F 3 −F 1) − (H 3 −H 1) in the sample were determined. When the skin temperature change amount (° C.) is less than 0.2 ° C., “weak effect”, when 0.2 ° C. or higher is “active”, and when 0.5 ° C. or higher is “sufficient action”, A case of 1.0 ° C. or higher was judged as “having a strong action”. In addition, the statistical analysis of each measurement result used the t-test or the Bonferroni multiple comparison test after one-way analysis of variance, and the significance level was set to less than 5%.

<Evaluation method of CNS activity>
Near-infrared spectroscopy (NIRS) was used for evaluation of the state of the central nervous system. A 16-channel NIRS optical imaging brain function measuring device was attached to the frontal area of the subject, and the oxygenated hemoglobin (Oxy-Hb) concentration in the prefrontal cortex at the time of inhalation of blank, control and sample aroma was measured. The Oxy-Hb concentration in the blank was O1, the Oxy-Hb concentration in the control was O2, and the Oxy-Hb concentration in the sample was O3. For the Oxy-Hb concentrations of all 16 channels, the Oxy-Hb concentration change ΔO = O2-O1 in the control and the Oxy-Hb concentration change ΔO = O3-O1 in the sample were determined. In addition, the statistical analysis of each measurement result performed the t test, and made the significance level less than 5%.

<Evaluation method of palatability for aroma>
The fragrance preference was evaluated. As for the preference of fragrance, subjective evaluation was obtained on a 100 mm line scale with “not at all” and “very preferable” at both ends. In addition, the statistical analysis of each measurement result performed Bonferroni's multiple comparison test after one-way analysis of variance, and the significance level was set to less than 5%.

<Test Example 1>
Psychological effects on mood and emotion, and effects on autonomic and central nervous system by inhalation of darjeeling fragrance were evaluated. The subjects were 17 women aged 25 to 40 years. During the daytime period (normal time when the central nerve is not excited or sedated), we were seated in a test room set at room temperature 23 ° C. and relative humidity 40%, and rested for 30 minutes to acclimatize to the test environment. In the state of inhaling aroma due to the vomiting action by natural breathing, the Oxy-Hb concentration in the prefrontal cortex was measured by pupillary photoreaction measurement, skin temperature measurement, and NIRS. The blank was breathed for 2 minutes, the control (Sayu) and the sample (Darjeeling fragrance) were presented under the nose for 2 minutes to inhale the fragrance. Samples were extracted from 10 g of Darjeeling (second flash) tea leaves with 600 mL of boiling mineral water for 4 minutes. Controls and samples were presented at a temperature of 85 ° C. Evaluation for mood by VAS and evaluation for emotion by MMS were measured before the test (blank) and after inhalation of darjeeling aroma.

The measurement results are shown below.
(1) Change in mood due to VAS FIG. 1 shows a change in mood due to VAS. The average VAS score before the test (blank) is “overall fatigue”, “spontaneous stress”, “boring”, “clean head”, “concentration”, “motivation” and “exhilaration” In any of the cases, it exceeded 30 mm, indicating that the subject before the test felt a moderate or higher stress. Comparing the scores of each item before and after inhalation of darjeeling fragrance, the scores of “overall fatigue”, “voluntary stress”, “boring” and “motivation” showed a decreasing tendency after inhalation, especially “spontaneous “Stress” was significantly reduced (p <0.01). On the other hand, “head refreshment”, “concentration” and “exhilaration” showed an upward trend, and particularly “exhilaration” increased significantly (p <0.01).

(2) Change of emotion by MMS FIG. 2 shows the change of emotion by MMS. Comparing the scores of each item before and after inhalation of darjeeling aroma, “depression / anxiety”, “hostility”, “fatigue”, “active”, “concentration” showed a decreasing tendency after inhalation, especially “depression / anxiety” “(P <0.01),“ hostility ”(p <0.05) decreased significantly. On the other hand, “inactive” and “affinity” showed an upward trend, and in particular, “inactive” increased significantly (p <0.01).

(3) Change in pupillary ratio due to measurement of pupillary light response FIG. 3 shows a change in pupillary ratio due to measurement of pupillary light response. The enlargement of the pupil is controlled by the sympathetic nerve, and the contraction is controlled by the parasympathetic nerve. When light stimulation is applied at rest (parasympathetic nerve dominant state), the sympathetic nerve activity is activated and the pupil is greatly enlarged. That is, the magnitude of the change in the miosis rate in this test means the presence or absence of the relaxed state before the light stimulation (the degree of relative superiority of the parasympathetic nerve activity in the autonomic nerve). Compared to Sayu, the change in miosis rate was significantly increased by inhalation of Darjeeling aroma (p <0.01). Therefore, it was shown that the darjeeling aroma regulates the autonomic nerve and has the effect of making the parasympathetic nerve activity relatively dominant.

(4) Skin temperature change by measuring skin temperature at peripheral site FIG. 4 shows the skin temperature change by measuring skin temperature at the peripheral site. Changes in skin blood flow accompanying expansion and contraction of peripheral blood vessels of the skin are governed by sympathetic nerve activity, and skin temperature fluctuates in response to changes in blood flow. That is, the magnitude of the change in skin temperature in this test means the strength of the inhibitory action on sympathetic nerve activity. Compared to Sayu, darjeeling aroma inhalation significantly increased skin temperature change (p <0.05), and skin temperature change exceeded 1.0 ° C. It was shown that Therefore, it was shown that the darjeeling fragrance has an action of extremely strongly suppressing sympathetic nerve activity.

(5) Changes in Oxy-Hb concentration in prefrontal cortex by NIRS FIG. 5 shows changes in Oxy-Hb concentration in prefrontal cortex by NIRS. Changes in Oxy-Hb concentration observed by NIRS reflect cerebral blood flow and brain activity. Compared to Sayu, the Oxy-Hb concentration tended to decrease in a wide range of prefrontal cortex due to inhalation of Darjeeling aroma. In particular, the Oxy-Hb concentration significantly decreased (p <0.05) in ch9, ch10, and ch12 near the left frontal orbit. From the above, it was shown that the central nervous system is sedated by inhalation of fragrance and has an effect of reducing cerebral blood flow in the prefrontal cortex.

(6) Summary As a psychological action of darjeeling (second flash) fragrance, “feeling tired”, “voluntary stress”, “depression / anxiety”, “hostility” It has been shown that “heavy feeling”, “freshness” and “affinity” are increased. As a physiological effect, it was shown that sympathetic nerve activity is strongly suppressed in the autonomic nervous system, and parasympathetic nerve activity is dominant. Moreover, it was shown that it has a sedative action which reduces cerebral blood flow in central nervous activity. Based on the above results, the darjeeling fragrance is a manifestation of primitive emotions (itchiness, anger, depression, anxiety, etc.) caused by physiological sedation and psychological stress in people who feel moderate stress. It was suggested that it has the effect of suppressing mental health and improving mental status.

<Test Example 2>
Using teas by production area with different aroma characteristics from Darjeeling (second flash), the preference for black tea aroma, the psychological effects of black tea aroma and the effects on autonomic nervous activity were evaluated and compared. The subjects were 13 women aged 25 to 40 years old. The subject was seated in a test room set at a room temperature of 23 ° C. and a relative humidity of 40%, and rested for 30 minutes to acclimatize to the test environment. The pupillary photoreaction measurement and skin temperature measurement were performed in the state in which the aroma was inhaled by the vomiting operation by natural breathing. The blank was breathed with air for 2 minutes, the sample was presented under the nose for 2 minutes, and the pupillary light response and skin temperature were measured. The sample used was extracted with 600 mL of boiling mineral water for 10 g of each tea leaf of Darjeeling (second flash), Assam, Uba, Kemon, Nuwara area and Dimbra. The extraction time was 4 minutes for Darjeeling and Keimon, 3 minutes for Assam, and 2 minutes 30 seconds for Uba, Dimbra and Nuwara areas. Samples were presented at a temperature of 85 ° C. Evaluation of palatability for aroma was performed after inhalation of aroma. The evaluation for mood by VAS and the evaluation for emotion by MMS were measured before the test (blank) and after inhalation of fragrance (only Darjeeling, Assam and Uva were performed).

The measurement results are shown below.
(1) Change in mood by VAS FIG. 6 shows changes in mood by VAS before and after inhalation of Darjeeling, Assam and Uva aromas. The average VAS score before the test (blank) is 46.5 mm for “overall fatigue”, 42.4 mm for “spontaneous stress”, 44.5 mm for “boring”, and “smooth head” 43.6 mm, “Concentration” was 44.2 mm, “Motivation” was 46.7 mm, and “Exhilaration” was 39.1 mm, which exceeded 30 mm in all items. It was shown that the person was feeling moderate stress or higher. In all black teas, “overall fatigue”, “spontaneous stress”, “dullness” tendencies to decrease after fragrance inhalation, “head refreshment”, “concentration”, “ A tendency to increase “motivation” and “exhilaration” was shown. In particular, with Darjeeling fragrance, “overall fatigue” and “spontaneous stress” were less than those of Assam and Uva.

(2) Change in pupillary ratio due to measurement of pupillary light response FIG. 7 shows the amount of change in pupillary ratio due to aroma inhalation of six black teas in different production areas. In all the black teas, the miosis rate increased after inhalation compared to before inhalation. In particular, in the fragrance of Darjeeling, Assam, Nuwara area, and Dimbra, the amount of change in the miosis rate was 0.05 or more, and it was shown that the autonomic nerve is regulated and the parasympathetic nerve activity is relatively dominant.

(3) Skin temperature change due to measurement of skin temperature at peripheral site FIG. 8 shows changes in skin temperature due to aroma inhalation of six teas with different production areas. In any black tea, the amount of change in skin temperature increased after inhalation compared to before inhalation. In particular, the change in skin temperature was 0.5 ° C. or more for Darjeeling and Assam fragrances, and 0.2 ° C. or more for Kemon, Nuwara Area and Dimbra fragrances. From the above, the fragrance of Darjeeling, Assam, Keamon, Nuwara area and Dimbra has the effect of suppressing sympathetic nerve activity, especially the fragrance of Darjeeling has the effect of suppressing sympathetic nerve activity superior to that of other teas It was shown that there is.

(4) Preference of fragrance Evaluation on the preference of fragrance of six teas with different production areas is shown in FIG. It was shown that the scent of Assam is the most palatable.

(5) Summary From the comparison results of fragrance preference, psychological mood change, miosis rate change amount and skin temperature change amount, Darjeeling fragrance is It has been shown that there is an autonomic nervous control effect superior to that of the production area, and this effect appears regardless of the preference for the darjeeling aroma.

<Test Example 3>
From Test Examples 1 and 2, it was shown that the fragrance of Darjeeling black tea has an excellent autonomic nervous regulation effect compared with the black tea of other production areas. Compared. The analysis subjects were 6 teas with different production areas (Darjeeling Second Flash, Assam, Uba, Dimbra, Nuwara Area and Keimon) used in Test Example 2, 1 tea production blend tea, and Darjeeling tea leaves by harvest from different tea gardens A to B. (First Flash and Second Flash) 6 points, 4 points of tea leaves with different quality (price) of Darjeeling and 3 points of different blends were made 19 points in total. An analysis sample was prepared by extracting with 2.5 mL of hot water for 4 minutes with 150 mL of hot water. The aroma component was collected by SPME and subjected to GC-MS analysis. Components were identified for 45 types of detected main peaks, and peak area values were measured. The results are shown in Tables 1-4. In addition, eight fragrance components characteristic of Darjeeling black tea were quantified. The groups according to each ingredient name and aroma characteristic are as follows.

  Each component is based on the sensory characteristics of the fragrance: (A) citrus flower-like, (B) sweet flower-like, (C) mellow flower-like, (D) woody-like, (E) green-like, and (F) cool-like Classified into groups. (A) Citrus flower-like linalool, linalool oxide (cis-furanoid type) (linalool oxide (cis-franoid)), linalool oxide (trans-furanoid type) (linalool oxide (trans-franoid)), linalool oxide ( cis pyranoid type) (linalool oxide (cis-pyranoid)), linalool oxide (trans-pyranoid type) (linalool oxide (trans-pyranoid)), hotrienol, citral, nerol, geraniol And (B) sweet flower-like, benzaldehyde, phenylacetaldehyde, β-damascenone, benzylalcohol, 2-phenyl, and a group consisting of α-terpineol and α-terpineol. From ethyl alcohol (2-phenylethylalcohol) and cis-jasmone Group (C) Hanahana-like is 6-methyl-5-hepten-2-one, β-cyclocitral, α-ionone ( α-ionone), β-ionone, and 5,6-epoxy-β-ionone, (D) Woody like β-myrcene ( β-myrcene), α-terpinene, γ-terpinene, D-limonene, α-phellandrene, cis-β-cymene (cis- β-ocimene)), trans-β-ocimene, p-cymene and terpinolene, (E) green-like is hexanal, trans- 2-hexen-1-al, cis-3-hexen-1-ol-acetate, hexanol hexanol), cis-3-hexen-1-ol (cis-3-hexen-1-ol), trans-2-hexen-1-ol and trans-3-butyric acid A group consisting of hexenyl (trans-3-hexenyl butyrate), (F) Cool was a group consisting of methyl salicylate.

  From Tables 1 to 4, (A) Citrus flower-like, (B) Sweet flower-like, (C) Rich flower-like, (D) Woody-like, (E) Green-like, and (F) Cool-like group fragrance components are the same The value (A)-(F) which added the peak area value of each component in a group, and the total value (X) of all the aroma groups were computed. The composition ratio of (A) citrus flower-like and (D) woody-like group aroma components (A) + (D) to the total value (X) is characteristic of high-quality Darjeeling black tea, 53% or more Was shown to be.

  Next, as a result of quantifying eight kinds of aroma components characteristic of darjeeling black tea, it was shown that darjeeling black tea has a much higher content of phototrienol and furaneol than other production areas. Moreover, both aroma components were contained more than other production areas even in low quality (low price) darjeeling black tea, and phototrienol was 0.01 ppm or more and furaneol was 0.02 ppm or more.

<Test Example 4>
Autonomic nervous activity of six kinds of aroma components (trans-2-hexene-1-al, phototrienol, methyl salicylate, geraniol, 2-phenylethyl alcohol and furaneol) and darjeeling black tea (second flash) characteristic of darjeeling black tea We evaluated and compared the effects on
A total of 5 subjects, 3 females in their 25s and 50s and 2 males, who were working full-time (under a mild to moderate chronic stress) were used as subjects. The subject was seated in a test room set at a room temperature of 23 ° C. and a relative humidity of 40%, and rested for 30 minutes to acclimatize to the test environment. The pupillary photoreaction measurement and skin temperature measurement were performed in a state in which aroma due to the vomiting action by natural breathing was inhaled. The blank was breathed with air for 2 minutes, the control (saline) and the sample were presented under the nose for 2 minutes, and the pupillary light response and skin temperature were measured. Eight kinds of fragrance component samples were prepared by diluting with ultrapure water so as to have a concentration similar to that of Example Product 1 using standard products of each component used in the fragrance component analysis. Darjeeling black tea was prepared in the same manner as in Test Example 3. Each sample was presented at a temperature of 85 ° C. The amount of change in miosis rate and the amount of change in skin temperature (° C.) in the control and the sample were determined. (A) Regarding the effect of relatively predominating parasympathetic nerve activity in the autonomic nerve, if the value obtained by subtracting the amount of change of the miosis rate of the control from the sample is less than 0.04, “weak effect”, 0.04 If it is less than 0.05, it is regarded as “acting”, and if it is 0.05 or more, it is regarded as “sufficient action” (parasympathetic activity evaluation). In addition, (i) regarding the strength to suppress sympathetic nerve activity, if the value obtained by subtracting the control skin temperature change amount (° C.) from the sample is less than 0.2 ° C., “weak effect”, 0.2 ° C. or more When the temperature was less than 0.5 ° C., it was judged as “acting”, when 0.5 ° C. or more and less than 1.0 ° C., “sufficient action”, and when it was 1.0 ° C. or more, it was judged as “strong action” (sympathetic nerve). Activity suppression evaluation). For the comprehensive evaluation regarding the autonomic nerve control action, the lower one of the evaluations (a) and (b) was adopted. The results are shown in Table 5.

  Evaluation of predominance of parasympathetic nerve activity in the autonomic nerve based on the amount of change in the miosis rate in Table 5 (parasympathetic nerve activity evaluation) (A) and evaluation of the suppression intensity of sympathetic nerve activity based on the skin temperature change (° C) (sympathetic nerve) From the results of (activity suppression evaluation) (a), it was shown that phototrienol and furaneol have an excellent autonomic nervous regulation effect compared to other aroma components.

<Test Example 5>
In Test Example 4, for the phototrienol and furaneol that were shown to have an excellent autonomic nerve regulating action, the effective concentrations of each component alone and when mixed were verified.
The subjects were four women in their 25s to 50s who were working full-time (under a mild to moderate chronic stress condition). The subject was seated in a test room set at a room temperature of 23 ° C. and a relative humidity of 40%, and rested for 30 minutes to acclimatize to the test environment. The pupillary photoreaction measurement and skin temperature measurement were performed in a state in which aroma due to the vomiting action by natural breathing was inhaled. The blank was breathed with air for 2 minutes, the sample was presented under the nose for 2 minutes, and the pupillary light response and skin temperature were measured. The sample was prepared by diluting with ultrapure water to a specified concentration using the standard phototrienol and furaneol used in the aroma component analysis. Each sample was presented at a temperature of 85 ° C. The amount of change in the miosis rate and the amount of change in skin temperature (° C.) in the sample were determined. (A) Regarding the effect of making the parasympathetic nerve activity relatively dominant in the autonomic nerve, if the miosis rate change amount in the sample is less than 0.04, “weak effect”, 0.04 or more and less than 0.05 In the case of “acting”, 0.05 or more is “sufficient effect”. In addition, (i) regarding the strength to suppress the sympathetic nerve activity, if the skin temperature change (° C) value in the sample is less than 0.2 ° C, “weak effect”, 0.2 ° C or more and less than 0.5 ° C In the case of “No effect”, 0.5 ° C. or more and less than 1.0 ° C. was judged as “sufficient action”, and 1.0 ° C. or more was judged as “strong action”. For the comprehensive evaluation regarding the autonomic nerve control action, the lower one of the evaluations (a) and (b) was adopted. Table 6 shows the evaluation results of the autonomic nerve regulating action at the concentrations of the components alone and mixed for phototrienol and furaneol.

<Test Example 6>
Each aroma water was prepared using Darjeeling 2nd flush and Uba tea leaves. After adding 1800 kg of warm water to 180 kg of each tea leaf, the tea leaf was removed with a filter, and 1600 kg of the extract was recovered. In the practical products 39 and 41, the extract was heated and concentrated under reduced pressure, and after collecting 1440 kg of steam water, 50 kg (Brix 0.09) of aromatic water was obtained by reverse osmosis membrane (RO membrane) treatment. The implementation product 40 used the said extract as it was, without passing through a concentration process among the said methods. The aroma components contained in the aroma water were analyzed by gas chromatography mass spectrometry.
About the aroma water prepared from said tea leaves, the effect on autonomic nerve activity was evaluated and compared.
Three females in their 40s and 50s who were working full-time (under a mild to moderate chronic stress condition) were subjects. The subject was seated in a test room set at a room temperature of 23 ° C. and a relative humidity of 40%, and rested for 30 minutes to acclimatize to the test environment. The pupillary photoreaction measurement and skin temperature measurement were performed in a state in which aroma due to the vomiting action by natural breathing was inhaled. The blank was breathed with air for 2 minutes, the sample was presented under the nose for 2 minutes, and after inhaling the sample for 1 minute, 10 mL of the sample was slowly swallowed over 30 seconds, and the pupillary photoreaction and skin temperature were measured after 30 seconds. The sample used was prepared by diluting the fragrance component prepared from the above-described black tea leaf with ultrapure water (Example 39 and Example 40 were diluted 50 times, and Example 41 was diluted 5 times). Each sample was presented at a temperature of 25 ° C. The amount of change in the miosis rate and the amount of change in skin temperature (° C.) in the sample were determined. (A) Regarding the action of making the parasympathetic nerve activity relatively dominant in the autonomic nerve, “if there is an action” when the miosis rate change amount in the sample is 0.04 or more and less than 0.05, “if it is 0.05 or more” It has sufficient action. In addition, (i) regarding the strength to suppress the sympathetic nerve activity, when the skin temperature change amount (° C.) value in the sample is 0.2 ° C. or higher and lower than 0.5 ° C., “effective”, 0.5 ° C. or higher 1 When the temperature was less than 0 ° C., it was judged as “sufficient action”, and when it was 1.0 ° C. or more, it was judged as “strong action”. For the comprehensive evaluation regarding the autonomic nerve control action, the lower one of the evaluations (a) and (b) was adopted. The results are shown in Tables 7 and 8.

  As a result of evaluating the action on the autonomic nerve activity of the aroma water prepared from the tea leaves, all of the aroma waters showed a sufficient autonomic nerve regulating action in any ingestion method of inhalation and drinking.

<Test Example 7>
Compressed by a tablet press in which erythritol (85% by mass), sorbitol (10% by mass), sugar ester (4% by mass), phototrienol (0.001% by mass), and furaneol (0.001% by mass) were mixed. An autonomic nerve control tablet containing furaneol was obtained. The effect on the autonomic nervous activity of taking tablets is reflected in the pupillary light of 3 females in their 25s to 40s who are working full-time (feeling mild to moderate chronic stress). When evaluated by reaction measurement and skin temperature measurement, the change in miosis rate was 0.11, indicating “sufficient action”, and the change in skin temperature (° C.) was 0.93 ° C. indicating “sufficient action”. The overall evaluation of the neuromodulation effect was “sufficient”.

The fragrance composition of the present invention exhibits an excellent autonomic nervous control effect, suppresses sympathetic nerve activity and enhances parasympathetic nerve activity, and has an action of suppressing emotional behavior in the central nervous system. Thereby, it exhibits an excellent effect as a medical assistance method for reducing, alleviating or preventing various symptoms due to physical or mental fatigue or stress, or for mental sedation. The aroma composition of the present invention can be widely used in various applications such as health functional foods, drinking water, and cosmetics.

Claims (17)

  An autonomic nervous regulator comprising one or more fragrance components derived from black tea leaves.   Aroma components derived from tea leaves are (A) citrus flowers, (B) sweet flowers, (C) mellow flowers, (D) woody, (E) green and (F) cool It is comprised from these. The autonomic nerve regulator of Claim 1 characterized by the above-mentioned. (A) Citrus flowers, (B) Sweet flowers, (C) Rich flowers, (D) Woody, (E) Green and (F) Cool The autonomic nerve regulating agent according to claim 2, comprising a component.
(A) linalool oxide (trans-franoid type) (linalool oxide (trans-franoid)), linalool oxide (cis-furanoid type) (linalool oxide (cis-franoid)), linalool (hotalenol), α-terpineol (Α-terpineol), linalool oxide (cis-pyranoid type) (linalool oxide (cis-pyranoid)), citral, linalool oxide (trans-pyranoid type) (linalool oxide (trans-pyranoid)), nerol (nerol) or Geraniol
(B) benzaldehyde, phenylacetaldehyde, β-damascenone, benzylalcohol, 2-phenylethylalcohol or cis-jasmone
(C) 6-methyl-5-hepten-2-one, β-cyclocitral, α-ionone, β-ionone (Β-ionone) or 5,6-epoxy-β-ionone (5,6-epoxy-β-ionone)
(D) β-myrcene, α-terpinene, D-limonene, α-phellandrene, trans-β-ocimene Γ-terpinene, cis-β-ocimene, p-cymene or terpinolene
(E) hexanal, trans-2-hexen-1-al, cis-3-hexen-1-ol, acetate, hexanol (Hexanol), cis-3-hexen-1-ol (cis-3-hexen-1-ol), trans-2-hexen-1-ol (trans-2-hexen-1-ol) or trans-2 butyric acid -Hexenyl (trans-3-hexenyl butyrate)
(F) Methyl salicylate The peak area ratio measured by gas chromatograph mass spectrometry (GC / MS analysis) of each aromatic component is {(A) + (D)} / {(A) + (B) + (C) + (D) + (E) + (F)} ≧ 0.53
The autonomic nerve regulating agent according to claim 2 or 3, wherein the condition is satisfied. The fragrance component contains 0.01 ppm or more of phototrienol, and the peak area ratio measured by gas chromatography mass spectrometry (GC / MS analysis) of each fragrance component is
{(B) + (C)} / {(A) + (B) + (C) + (D) + (E) + (F)} ≦ 0.28
Or {(E) + (F)} / {(A) + (B) + (C) + (D) + (E) + (F)} ≦ 0.29
The autonomic nerve regulating agent according to claim 2 or 3, wherein the condition is satisfied.   The autonomic-nerve regulator of any one of Claims 1-5 containing 0.1-10 ppm of phototrienol.   The autistic component according to any one of claims 2 to 6, further comprising (G) a sweet-like component as an aroma component.   (G) The autonomic nerve regulating agent according to claim 7, wherein the sweet-like component is furaneol. The autonomic nerve regulating agent according to claim 8, comprising 0.007 to 1 ppm of furaneol.   10. The autonomic nervous regulator according to any one of claims 3 to 9, which contains 0.2 to 4.8 ppm of methyl salicylate. The autonomic nerve regulator according to any one of claims 3 to 10, comprising 0.1 to 12.7 ppm of linalool.   The autonomic nerve modulating agent according to any one of claims 1 to 11, which is an autonomic nerve regulating action by sedation of the central nervous system.   An autonomic nervous regulator characterized by comprising phototrienol and / or furaneol as an active ingredient.   The autonomic nerve regulating agent according to claim 13, comprising 0.05 to 100 ppm of phototrienol and / or 0.02 to 10 ppm of furaneol.   The autonomic nervous modulator according to claim 13 or 14, comprising phototrienol and furaneol, containing 0.05 ppm or more of phototrienol, and the total of phototrienol and furaneol being 0.1 ppm or more.   The pharmaceutical for an autonomic nerve adjustment containing the autonomic-nerve regulator of any one of Claims 1-15, a functional food, a supplement, food-drinks, or cosmetics. A method for reducing, alleviating, preventing or recovering stress symptoms, or a method for sedating a psychiatric disorder, characterized by causing a human to ingest the autonomic nervous modulator of any one of claims 1 to 15 from the skin, nasally or orally.