JP2019137660A - Citrus yoko-containing composition - Google Patents

Abstract

To provide a new use for Citrus Yoko, namely, a composition available for pharmaceuticals, food and drink, cosmetic preparations, daily necessaries or the like.SOLUTION: The present invention provides an antibacterial composition, an intestinal regulation composition, a composition for preventing and/or improving periodontitis and/or oral malodor, a composition for preventing and/or improving diabetes, and a food composition, containing Citrus Yoko fruit juice, pericarp, pericarp extract or fruit juice squeezed lees extract, as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a composition containing Yuko or an extract thereof as an active ingredient. The present invention is also useful as an antibacterial composition, an intestinal composition, a composition for preventing and improving periodontal disease and / or halitosis, and a composition for preventing and improving diabetes. Can also be useful as food and beverage products, cosmetics, personal care products and household care products.

  Yuko (Citrus Yoko) is a citrus citrus fruit of the citrus family, and is a natural hybrid of Yuzu and Daidai, and is known as the original species of Sudachi. Yuko has poor cold resistance compared to Yuzu and Sudachi, and its production is limited to a specific area centering on Tokushima Prefecture, resulting in low production and low national recognition. For this reason, there have been few nutritional and food studies on Yuko.

  Although the composition and active ingredients of the natural material Yuko are not clearly elucidated, Yuko is considered to have characteristics not found in other citrus fruits such as Yuzu and Sudachi. Therefore, Yuko is considered to have a different use from other citrus fruits.

  As for Yuko, it has been reported so far that it can be used in hair restorers and cosmetics, but there is no particular report on other uses except that it is used for component comparison with other citrus fruits (Non-patent Document 1). -2 and patent documents 1-2).

Japanese Patent Laid-Open No. 07-126129 Japanese Patent Laid-Open No. 07-126142

Yoshiaki Miyake, Journal of Japanese Society of Food Life, Vol. 26, No. 2, 71-78 (2015) Masao Tsukayama et al., Japanese Journal of Food Science and Technology, Vol. 56, No. 6, 359-362 (2009)

  The present invention finds a new use of Yuko, which is not well known nationwide and is mostly treated as waste, and provides a composition that can be used in a wide range of fields such as pharmaceuticals, foods and drinks, cosmetics, and daily necessities. For the purpose.

As a result of intensive studies to solve the above problems, the present inventors have found that Yuko, particularly Yuko Juice, has antibacterial activity, and further, as an accompanying effect,
(1) Improves disturbance of the intestinal microflora due to a high-fat diet. Specifically, it reduces lactic acid bacteria and bifidobacteria, which are good bacteria, and increases Clostridium, which is bad bacteria. Suppresses and improves intestinal flora,
(2) It leads to an increase in Akkermansia that has anti-diabetic and anti-obesity effects,
(3) It has been found that it has various excellent effects applicable to practical use such as suppressing the growth of oral bacteria and the concentration of oral volatile sulfides, and (4) improving high fat diet-induced abnormal glucose tolerance. The present invention has been completed.

That is, the present invention provides the following aspects.
[1] An antibacterial composition containing yuko or an extract thereof as an active ingredient.
[2] The antibacterial composition according to [1], wherein the Yuko or an extract thereof is Yuko's fruit juice, pericarp, pericarp extract or fruit juice extract.
[3] The antibacterial composition according to [2], wherein the fruit juice is juice obtained by squeezing fruit (original fruit juice), fruit juice obtained by diluting the original fruit juice, concentrated fruit juice, or concentrated reduced fruit juice.
[4] A composition for intestinal regulation containing yuko or an extract thereof as an active ingredient.
[5] The composition for intestinal regulation according to [4], which improves deterioration of intestinal bacterial flora due to a high-fat diet.
[6] The composition for intestinal regulation according to [4] or [5], which increases Ackermancia bacteria in the intestine.
[7] A composition for preventing and / or improving periodontal disease and / or halitosis, comprising yuko or an extract thereof as an active ingredient.
[8] A composition for preventing and / or improving diabetes, comprising yuko or an extract thereof as an active ingredient.
[9] The composition for preventing and / or improving diabetes according to [8], which improves high fat diet-induced glucose tolerance abnormality.

[10] A food composition containing yuko or an extract thereof as an active ingredient.
[11] The food composition according to [10], wherein Yuko or an extract thereof is Yuko juice, fruit skin, fruit skin extract or fruit juice extract.
[12] The food composition according to [11], wherein the fruit juice is juice obtained by squeezing fruit (original fruit juice), fruit juice obtained by diluting the original fruit juice, concentrated fruit juice, or concentrated reduced fruit juice.
[13] The food composition according to any one of [10] to [12] for improving deterioration of intestinal bacterial flora due to a high fat diet.
[14] The food composition according to [13], which increases Ackermancia bacteria in the intestine.
[15] The food composition according to any one of [10] to [12] for preventing and / or improving periodontal disease and / or bad breath.
[16] The food composition according to any one of [10] to [12] for preventing and / or improving diabetes.
[17] The food composition according to [16], which improves high fat diet-induced glucose tolerance abnormality.

Furthermore, the present invention provides the following aspects.
[18] A food, beverage, cosmetic, personal care product or home care product comprising the antibacterial composition according to any one of [1] to [3].
[19] A method for preserving food and drink, comprising blending the antibacterial composition according to any one of [1] to [3] into the food or drink.
[20] A method for preserving a cosmetic, comprising blending the antibacterial composition according to any one of [1] to [3] into the cosmetic.
[21] A method for improving deterioration of intestinal microflora due to a high-fat diet, comprising administering a therapeutically effective amount of Yuko or an extract thereof to a patient in need of treatment.
[22] A method for treating periodontal disease and / or halitosis, comprising administering a therapeutically effective amount of Yuko or an extract thereof to a patient in need of treatment.
[23] A method for treating diabetes, comprising administering a therapeutically effective amount of Yuko or an extract thereof to a patient in need of treatment.
[24] Use of Yuko or an extract thereof in the manufacture of a medicament for improving deterioration of intestinal flora caused by a high fat diet.
[25] Use of Yuko or an extract thereof in the manufacture of a medicament for the prevention and / or treatment of periodontal disease and / or bad breath.
[26] Use of Yuko or an extract thereof in the manufacture of a medicament for preventing and / or treating diabetes.

  According to the present invention, Yuko or an extract thereof effectively suppresses the growth of bacteria and fungi such as Escherichia coli and Staphylococcus aureus, so that it can be applied to pharmaceuticals, foods, home care products and the like using the antibacterial action. Can be expected, and further, the effects associated therewith, that is, the action of regulating the intestines, the prevention and / or improvement of periodontal disease and / or bad breath, the prevention and / or improvement of diabetes, to various drugs, foods, etc. Use is also expected. In particular, Yuko is a natural fruit, so it is highly safe and widely applicable.

Figure 1 shows each group (ordinary food + 100% fruit juice administration group, normal meal + saline administration group, high fat diet (HFD-60%) + 100% fruit juice administration group, high fat diet + saline) The composition of the intestinal microflora in (administration group) is shown. FIG. 2 shows (A) inhibition circle for E. coli strain K12 (Yuko fruit juice 30%, 60% and 100%), (B) inhibition circle for S. aureus (Yuko fruit juice 100%, Sudachi fruit juice 100% and Yuzu fruit juice). 100%), (C) Inhibition circles for baker's yeast (fungi) (Yuko juice 100% and Yuzu juice 100%). FIG. 3 shows (A) the number of colonies of Escherichia coli in various concentrations of Yuko juice, benzoic acid, and acetic acid, and (B) the number of colonies of Alicyclobacillus acidele terrestris in various concentrations of Yuko juice, benzoic acid, and acetic acid. Indicates. FIG. 4 shows (A) the number of bacteria in the oral cavity (periodontal disease bacteria count) before and after intake of Yuko juice, and (B) human β which is an antimicrobial peptide before and after intake of Yuko juice. -Indicates the amount of defensin 1 (pg / ml). FIG. 5 shows the ratio of the number of bacteria after the addition of Yuko soap solution (0.1 g / 100 μL) when the number of bacteria at normal times is 100%. FIG. 6 shows blood glucose levels after 0 to 120 minutes of glucose load in the Yuko administration group and the physiological saline administration group (control group).

  The present invention is described in detail below.

  In the present invention, “Yuko” refers to fruit juice, peel or lyophilized powder thereof derived from Citrus Yuko. Fruit juice, pericarp or lyophilized powder thereof can be appropriately used depending on the purpose.

The fruit juice of Yuko is not particularly limited as long as it is a fruit juice obtained from Yuko. In addition, the fruit juice of Yuko may be diluted with a solvent such as water or ethanol. The fruit juice can be used as, for example, juice obtained by squeezing fruit (original fruit juice), fruit juice obtained by diluting the original fruit juice, concentrated fruit juice, and concentrated reduced fruit juice.
Concentrated fruit juice refers to the original fruit juice that is obtained by removing the moisture in the fruit juice by the heating concentration method or the freezing concentration method, etc., and increasing the concentration of the fruit juice. Fruit juice diluted with a solvent such as water so as to have a concentration equivalent to the original fruit juice.

  The fruit juice of Yuko may be obtained by squeezing a fruit juice and a fruit juice. Examples of the squeezing method include a method of squeezing by hand and a method of squeezing with a device generally used in the field, but are not limited thereto.

In the present invention, the “Yuko extract” is obtained by extracting from Yuko with a variety of solvents. Examples of the Yuko extract include a peel extract and a fruit juice extract.
Examples of the extraction method include, but are not limited to, a hot water extraction method, a boiling extraction method, an immersion extraction method, a shaking extraction method, a Soxhlet extraction method, and a steam distillation method.

  As the extraction solvent, for example, water, an organic solvent, or a mixed solvent thereof can be used. Examples of the organic solvent include lower alcohols such as ethanol, chloroform, ethyl acetate, and n-hexane. Preference is given to water and ethanol. Two or more of these solvents may be mixed and used.

  The use amount of the extraction solvent can be appropriately determined depending on the part of the yeast, the type of the extraction solvent, and the like. Moreover, extraction time is 1 minute-50 days, for example, Preferably it is 1 hour-30 days, Extraction temperature is 0 degreeC-100 degreeC, for example, Preferably it is 10 degreeC-80 degreeC.

  The Yuko extract can be used as it is, and can be further purified as necessary. The purification treatment may be performed by an ordinary method, and can be purified by, for example, filtration or chromatography. Then, the obtained filtrate can also be made into the dried product of Yuko by concentrating under reduced pressure, freeze-drying, etc.

  The “antibacterial composition” in the present invention refers to a composition having effects such as bacteriostatic (inhibition of bacterial growth), sterilization, sterilization, disinfection, sterilization, antiseptic and antifungal. Examples of the subject include bacteria and fungi, but are not limited thereto.

  Examples of bacteria include Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, Salmonella, Moraxella, Legionella; Gram-positive bacteria such as Staphylococcus aureus and Clostridium bacteria. Examples of fungi include yeasts such as Candida, Rhodotorula and baker's yeast; and molds such as red mold and black mold.

  The “intestinal composition” in the present invention refers to a composition that improves the intestinal function to a normal state. For example, it refers to a composition that improves the composition of the intestinal flora that has deteriorated by ingesting a high-fat diet (a state in which bad bacteria are increasing) to the normal composition of the intestinal flora.

  In the present invention, “deterioration of intestinal flora” refers to a state in which lactic acid bacteria and bifidobacteria belonging to good bacteria are decreased and Clostridium perfringens and staphylococci belonging to bad bacteria are increased. It has been reported that intestinal environment changes depending on dietary content such as excessive energy intake, increased lipid intake, and lack of exercise, especially dietary food (animal protein and saturated fat). It is known that bad bacteria increase and good bacteria decrease compared to plant foods.

  In the present invention, “a composition for preventing and / or improving periodontal disease and / or bad breath” refers to oral bacteria that cause periodontal disease (periodontal bacteria), for example, Streptococcus genus, Veillonella genus, Prevotella genus, Actinomyces It refers to a composition that suppresses the increase in the genera, genus Rothia, and Granulicatella, and reduces the concentration of hydrogen sulfide and methyl mercaptan that cause bad breath. Also included are compositions that increase antimicrobial peptides (eg, human β-defensin 1) in the oral cavity.

  In the present invention, “a composition for preventing and / or improving diabetes” refers to a composition for preventing, ameliorating and / or treating a disease causing diabetes and a subjective symptom associated therewith. For example, it refers to a composition that improves glucose intolerance caused by continuing to consume a high fat diet.

  The “high fat diet-induced impaired glucose tolerance” in the present invention refers to insulin resistance caused by continuing a diet high in lipids. As insulin resistance progresses, it becomes type 2 diabetes.

  “Improvement” in the present invention means improvement of disease symptom / state, prevention or delay of deterioration of disease symptom / state, reversal, prevention or delay of progression of disease symptom, or treatment of disease. "Means prevention or delay of the onset of the disease in the application subject or lowering the risk of the onset of the disease of the application subject." The “treatment” in the present invention means all treatments of diseases (for example, improvement, reduction, suppression of progression, etc.). It may also include prevention of disease onset and / or progression.

  The “patient” in the present invention means humans and other animals such as dogs, cats, horses and the like. The patient is preferably a mammal, more preferably a human.

  The “therapeutically effective amount” in the present invention means the amount of an active ingredient that induces a biological or pharmaceutical response required by a researcher or doctor in a tissue, system, animal or human. “Therapeutically effective amount” also encompasses the meaning of the amount of active ingredient that, when administered to a mammal for treating a disease, is sufficient to effect such treatment for the disease. To do. “Therapeutically effective amount” will vary depending on the active ingredient, the disease and its severity and the age, weight, etc., of the mammal to be treated.

  Furthermore, “effective amount” encompasses the amount of an active ingredient that, when administered at an appropriate dose, produces an acute or chronic therapeutic effect. This therapeutic effect includes the prevention, correction, inhibition, or reversal of the symptoms / signs and underlying pathology of the disease / condition and associated complications to a detectable extent.

  The “food composition” in the present invention is other than a pharmaceutical composition and may be any form that can be taken orally, such as a solution, suspension, emulsion, powder, solid molded product, and the like. Is not to be done.

  Examples of the food composition include instant foods such as instant noodles, retort foods, canned foods, instant soups and miso soups, freeze-dried foods; beverages such as soft drinks, vegetable drinks, powdered drinks, concentrated drinks, and alcoholic drinks; Flour products such as bread, noodles and bread crumbs; sweets such as strawberries, caramel, chewing gum, chocolate, cookies, biscuits, cakes, crackers, Japanese confectionery, dessert confectionery; seasonings such as sauces, dressings, soups; butter, margarine Fats and oils such as mayonnaise, dairy products such as milk drinks, yogurts, ice creams and creams; agricultural processed products such as jams, marmalades and cereals; frozen foods, etc. is not.

  The antibacterial composition of the present invention may be used in personal care products and household care products. That is, personal care products and household care products containing the antibacterial composition of the present invention can also be provided.

  Examples of personal care products containing the antibacterial composition of the present invention include soaps, hand soaps, body cleansers, mouth cleansers, toothpastes, shampoos, conditioners, hair styling agents, antiperspirants, body lotions, deodorants and the like. However, it is not limited to these.

  Examples of household care products containing the antibacterial composition of the present invention include clothing detergents, softeners, deodorants, fabric cleaning compositions, dishwashing detergents, automatic dishwashing detergents, floor care compositions, and kitchen cleaners. , Kitchen disinfectants, bathroom cleaners, bathroom disinfectants and the like, but are not limited to these.

  In addition, since Yuko used in the antibacterial composition of the present invention is a natural material, it may be blended in foods and drinks or cosmetics and used as a preservative.

  The method of using the antibacterial composition of the present invention as a preservative is not particularly limited as long as it is added by a suitable method to foods and beverages and cosmetics.

  Examples of the food and drink containing the antibacterial composition of the present invention include, but are not limited to, sprinkles added to cooked rice and toppings and jams added to breads. Further, examples of cosmetics containing the antibacterial composition of the present invention include basic cosmetics such as creams, emulsions, lotions, face wash, packs, and makeup cosmetics such as lipsticks and foundations, but are not limited thereto. It is not a thing.

  The blending amount of the antibacterial composition of the present invention in a food or drink or cosmetic varies depending on the type of the food or drink or cosmetic, and an amount corresponding to the purpose may be added.

  The composition of the present invention only needs to contain yuko or an extract thereof as an active ingredient, and may further contain excipients and the like as long as the effects of yuko or the extract are not hindered. Therefore, the ratio of Yuko or its extract in the composition of the present invention is not particularly limited. Alternatively, the composition of the present invention may consist only of Yuko or an extract thereof.

  The composition of the present invention can be administered orally or parenterally (eg, gastric, enteral). The composition of the present invention can be used as a pharmaceutical or food composition.

  In the present invention, when preparing the composition of the present invention as a pharmaceutical composition (pharmaceutical, quasi-drug, etc.), Yuko or its extract, other medicinal ingredients, pharmaceutically acceptable carriers and additions An agent can be blended. For example, as a pharmaceutically acceptable carrier and additive, a binder, a disintegrant, a lubricant, a wetting agent, a buffer, a fragrance, and the like can be mixed to prepare a desired dosage form. There are no particular limitations on the pharmaceutical dosage form, but injections, external preparations, inhalants, suppositories, films, lozenges, solutions, powders, tablets, granules, capsules, syrups, eye drops Agents, eyewashes, and nasal drops.

  In the present invention, the composition of the present invention is prepared as a food composition (health food, functional food, nutritional supplement, supplement, health functional food, food for specified health use, nutrition functional food, functional indication food, etc.). In the case of Yuko or its extract, sweetener, coloring agent, thickener, stabilizer, gelling agent, glue, antioxidant, color former, bleaching agent, yeast food, gum base, flavor, acidulant, A desired form can be prepared by mixing a seasoning, an emulsifier, a pH adjuster, a kansui, a swelling agent, a nutrient enhancer, or other food or beverage ingredients.

  Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

Example 1: Examination of inhibitory effect on deterioration of intestinal bacterial flora by high-fat diet with yuko juice (1) Method Six-week-old male C57B16 mice (Japan SLC; n = 20) were treated under SPF environment. Raised for a week. Eight-week-old C57B16 mice randomly divided into 4 groups (normal diet group (100% juice juice administration), normal diet group (saline administration), high fat diet (HFD-60%) group (100% juice juice administration) ) And high fat diet group (administered with physiological saline)) (n = 5 for each experimental group), and mice of each group were orally administered daily for 7 days with 200 μL of yuko fruit juice 100% or physiological saline. Seven days later, feces were collected, DNA was extracted, and intestinal flora analysis was performed by T-RFLP method at ICLAS (Central Laboratory for Experimental Animals) to analyze the intestinal flora.

(2) Results The results are shown in FIG. FIG. 1 shows the composition of the intestinal flora in each group.
In the high-fat diet group, lactic acid bacteria and bifidobacteria are decreased, Clostridium bacteria are increased, and the composition of the intestinal flora is deteriorated compared to the normal diet group. In the normal diet group and the high fat diet group, the composition deterioration of the intestinal microflora was improved by administering Yuko juice. Therefore, it was proved that Yuko juice has an effect of improving intestinal function.
In addition, the appearance of Ackermancia fungi, which is considered to have anti-diabetic effects in both the normal diet group and the high-fat diet group, was observed in the Yuko fruit juice administration group.

Example 2: Examination of antibacterial effect of Yuko juice The growth inhibition circle formation on E. coli strain K12, Staphylococcus aureus and baker's yeast (fungus) was observed on a normal agar medium, and the antibacterial activity of Yuko juice was evaluated.
(1) Method
(1-i) Infiltrate Yuko juice (30%, 60%, or 100%) into a circular filter paper to make Yuko juice-containing discs, and place each disc on a plate seeded with Escherichia coli K12. Culture was performed for a time, and the formed inhibition circle was observed.
(1-ii) Place each disk soaked with 100% fruit juice of Yuko, Sudachi or Yuzu on a plate seeded with Staphylococcus aureus, incubate at 37 ° C for 24 hours, observe the formed inhibition circle, And antibacterial activity compared with other citrus fruits. In addition, each disk soaked with 100% fruit juice of Yuko or Yuzu was placed on a plate seeded with baker's yeast (fungus), cultured at 37 ° C. for 24 hours, and the formed inhibition circle was observed.

(2) Results The results are shown in FIG. FIG. 2 shows (A) inhibition circle for E. coli strain K12, (B) inhibition circle for S. aureus, and (C) inhibition circle for baker's yeast (fungus).
Yuko Juice showed clear circles of inhibition against E. coli strain K12, Staphylococcus aureus and fungi. On the other hand, in Sudachi juice and Yuzu juice, no inhibition circle was observed against Staphylococcus aureus and fungi. That is, it is recognized that Sudachi juice and Yuzu juice cannot sufficiently suppress the growth of bacteria and fungi.

  From the above results, it was proved that Yuko Juice has an excellent antibacterial effect and further has an antibacterial effect superior to other citrus fruits.

Example 3: Comparative examination of antibacterial effect between Yuko juice and existing preservatives Yuko juice (1%, 5%) for E. coli and resistant thermoacidophilic bacteria (AAT; Alicyclobacillus acid telestris) , 25%, 50%, 75% and 100%), benzoic acid (60 ppm, 125 ppm, 190 ppm and 250 ppm) and acetic acid (0.15%, 0.23% and 0.30%) were compared and examined.
(1) Method 100% of Yuko juice was diluted with distilled water to prepare diluted solutions of 1%, 5%, 25%, 50% and 75% of Yuko juice. Benzoic acid and acetic acid were used as controls. Various concentrations of Yuko juice, benzoic acid and acetic acid were cultured with 10 ³ E. coli or AAT at 37 ° C. for 16 hours, and the number of colonies formed on the plate was counted.

(2) Results The results are shown in FIG. FIG. 3 shows (A) the number of colonies formed from E. coli juice, benzoic acid and acetic acid on E. coli, and (B) the number of colonies formed from A. umbilical juice, benzoic acid and acetic acid on AAT bacteria.
As with benzoic acid and acetic acid used as preservatives for food and drink, colony formation was hardly observed in the 50% diluted juice, 75% diluted solution, and 100% dried juice. It is recognized that That is, when the concentration of Yuko juice is 50% to 100%, it exhibits an antibacterial action, suggesting that Yuko juice having a concentration of 50% to 100% can be used as a preservative for foods and drinks.

Example 4: Examination of improvement effect of oral environment by Yuko Juice Method Method Healthy subjects were allowed to continuously take 50cc Yuko Juice 100% for one week, and oral volatile sulfides (hydrogen sulfide and methyl before and after ingestion). The concentration of mercaptans was measured with oral chroma (manufactured by Nippon Dental Trading Co., Ltd.), which is a breath odor measuring device (VSC monitor). The same measurement was performed 7 times.
Furthermore, saliva was collected from healthy individuals, DNA was extracted from the collected saliva samples, and the number of bacteria in the oral cavity (periodontal disease bacteria number) was analyzed by the T-RFLP method at Biotechnological Co., Ltd.
Further, the amount of human β-defensin 1 which is an antibacterial peptide was measured with a human beta defensin 1 ELISA kit (manufactured by Signal Way Antibody).

上記の結果から、ユコウ果汁を摂取することにより、口臭の原因である硫化水素およびメチルメルカプタンの濃度が有意に低下した。そのため、ユコウ果汁には口臭抑制効果があると認められる。 (2) Results Table 1 below shows average values of oral volatile sulfides (hydrogen sulfide and methyl mercaptan) before the intake of Yuko juice and after one week of intake.

From the above results, the concentration of hydrogen sulfide and methyl mercaptan, which cause bad breath, was significantly reduced by ingesting Yuko juice. Therefore, it is recognized that Yuko juice has a bad breath suppression effect.

The result regarding the improvement effect of periodontal disease is shown in FIG. FIG. 4 shows (A) the number of bacteria in the oral cavity (periodontal disease bacteria count) before and after intake of Yuko juice, and (B) human β which is an antimicrobial peptide before and after intake of Yuko juice. -Indicates the amount of defensin 1 (pg / ml).
By continuously ingesting Yuko juice for 1 week, periodontal bacteria Streptococcus genus, Veillonella genus, Prevotella genus, Actinomyces genus, Rothia genus and Granulicatella genus are significantly reduced, and antibacterial peptide human β-defensin 1 The amount of was significantly increased. That is, it was proved that Yuko juice has an effect of improving periodontal disease.

  From the above results, it is recognized that Yuko juice can improve the oral environment by improving bad breath and periodontal disease.

Example 5: Examination of bacteriostatic effect of Yuko soap (1) Method A natural soap containing 3% yuko juice, provided by a clean company of limited company, is dissolved in distilled water, and yuko soap solution (0.1 g / 100 μL) was prepared. The prepared Yuko soap solution and 10 ³ E. coli were mixed and cultured with shaking at 37 ° C. for 1 hour, and the number of E. coli was measured.

(2) Results The results are shown in FIG. FIG. 5 shows the ratio of the number of bacteria after the addition of Yuko soap solution (0.1 g / 100 μL) when the number of bacteria at normal times is 100%.
It was confirmed that the number of bacteria decreased by about 20% by adding Yuko soap solution.

Example 6: Examination of improvement effect of high fat diet-induced abnormal glucose tolerance by Yuko Juice (1) Method Six-week-old C57B16 mice (purchased from Japan SLC) were given a high fat diet (HFD-60) for 28 days. At the same time, 200 μL of yuko 100% was orally administered once a day for 28 days (n = 6). In the control group, physiological saline was administered instead of 100% of Yuko juice (n = 6). After 28 days, they were fasted overnight and an oral glucose tolerance test was performed.

(2) Results The results are shown in FIG. FIG. 6 shows blood glucose levels after 0 to 120 minutes of glucose load in the Yuko administration group and the physiological saline administration group (control group).
In the oral glucose tolerance test, the blood glucose level 30 minutes after glucose loading was significantly lower in the Yuko administration group than in the control group. This result indicates that Yuko juice has an effect of improving high fat diet-induced abnormal glucose tolerance.
During the 4 weeks, there was no significant difference in body weight between the Yuko juice administration group and the physiological saline administration group.

  Since the present invention uses Yuko or an extract thereof as an active ingredient, it is useful not only as a pharmaceutical composition but also as a food composition such as a food and drink, a supplement, and a care product. It is possible to provide a highly safe composition.

Claims (17)

  An antibacterial composition comprising yuko or an extract thereof as an active ingredient.   The antibacterial composition according to claim 1, wherein Yuko or its extract is Yuko's fruit juice, pericarp, pericarp extract or fruit juice extract.   The antibacterial composition according to claim 2, wherein the fruit juice is a juice obtained by squeezing a fruit (original fruit juice), a juice obtained by diluting the original fruit juice, a concentrated fruit juice, or a concentrated reduced fruit juice.   A composition for regulating the intestine, containing Yuko or an extract thereof as an active ingredient.   The composition for intestinal regulation according to claim 4, which improves the deterioration of intestinal bacterial flora caused by a high fat diet.   The composition for intestinal regulation according to claim 4 or 5, which increases Ackermancia bacteria in the intestine.   A composition for preventing and / or improving periodontal disease and / or halitosis, comprising yuko or an extract thereof as an active ingredient.   A composition for preventing and / or improving diabetes, comprising yuko or an extract thereof as an active ingredient.   9. The composition for preventing and / or improving diabetes according to claim 8, which improves a high fat diet-induced glucose tolerance abnormality.   A food composition containing Yuko or an extract thereof as an active ingredient.   The food composition according to claim 10, wherein Yuko or an extract thereof is Yuko fruit juice, fruit skin, fruit skin extract or fruit juice extract.   The food composition according to claim 11, wherein the fruit juice is a juice obtained by squeezing the fruit (original fruit juice), a juice obtained by diluting the original fruit juice, a concentrated fruit juice, or a concentrated reduced fruit juice.   The food composition according to any one of claims 10 to 12, for improving deterioration of intestinal microflora due to a high fat diet.   14. A food composition according to claim 13, characterized in that it increases Ackermancia fungi in the intestine.   The food composition according to any one of claims 10 to 12, for preventing and / or improving periodontal disease and / or bad breath.   The food composition according to any one of claims 10 to 12, for preventing and / or improving diabetes.   The food composition according to claim 16, which improves high fat diet-induced glucose tolerance abnormality.

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