JP5109117B2 - Sudachi-derived composition, and pharmaceutical composition, health food and drink and supplement containing the composition - Google Patents

Description

  The present invention relates to a composition derived from sudachi, and a pharmaceutical composition, health food and drink and supplement containing the composition, and in particular, freeze-dried pericarp of citrus (Citrus sudachi Hort), pulp and seed after squeezed juice The present invention relates to a composition containing a substance obtained by pulverization and a health food or drink containing the composition.

  Diabetes is a disease in which genetic predisposition and environmental factors are intricately involved, and can be broadly classified into type I diabetes caused by an insufficient amount of insulin due to insufficient secretion of insulin and type II diabetes caused by insufficient action of insulin. Among these diabetes types, type II diabetes is also called adult type diabetes and accounts for 90% of all diabetes. The main cause of type II diabetes is characterized by a decrease in insulin secretion from pancreatic β cells and a decrease in insulin action (insulin resistance). The number of type II diabetes patients has been increasing in recent years, and it is said that there are more than 7 million patients in Japan and more than 100 million patients worldwide. Furthermore, the number of patients is expected to double over the next 10 years. Social complications such as the frequent occurrence of complications (diabetic retinopathy, diabetic nephropathy, diabetic neuropathy) caused by diabetes and increased medical expenses It is becoming a serious problem.

  The main cause of this rapid increase in diabetes is presumed to be due to changes in environmental factors rather than genetic predispositions, especially obesity due to dietary nutrition, lack of exercise, and stress. Although the actual state and the cause of insulin resistance associated with obesity are not yet fully understood, it is thought to be due to a failure of the intracellular insulin signal transduction mechanism. Regarding the enhancement of insulin action in peripheral tissues, for example, in the soleus muscle of the hind limbs of Wistar fatty rats, the action of insulin (glycogen synthesis and glycolysis enhancement action) is enhanced (ex vivo), and around the accessory testicles of Wistar fatty rats In isolated adipocytes derived from adipose tissue, it has been reported that the action of insulin (the action of enhancing glucose oxidation and total lipid synthesis) is enhanced (ex vivo) (Sugiyama Y. et al .: Arzneim.-Forsch./ Drug Res., 40 (I), 3: 263, 1990.).

  Conventionally, a sulfonylurea agent (SU agent) and a group of drugs called biguanide drugs have been used as therapeutic agents for the above-mentioned type II diabetes. Recently, a drug called “insulin resistance improving drug” (pioglitazone), which improves insulin resistance and treats diabetes, is put on the market and is expected to become the mainstream of future diabetes treatment.

Sugiyama Y. et al .: Arzneim.-Forsch./Drug Res., 40 (I), 3: 263, 1990.

  However, the sulfonylurea agent increases insulin in the blood by promoting insulin secretion from the pancreas, promotes glucose uptake into peripheral tissues and organs, and lowers blood glucose level. There is concern about the transition to type I diabetes due to long-term use in order to work. In addition, the biguanide drugs have a risk of developing serious side effects such as hypoglycemia.

  By the way, Sudachi (English name = Sudachi, scientific name = Citrus sudachi Hort, ex Shirai family name = citrus family) is a related species of "Yuzu", and is a citrus citrus that Tokushima Prefecture accounts for 95% of the national production. Sudachi is consumed as a raw material for processed foods in addition to the use of raw food. However, there are currently no effective methods for using the residue, such as the peel and the fruit squeezed koji / seed. Such natural materials are generally excellent in intestinal absorption, have no side effects, and are highly stable to the human body. However, until now, sufficient studies have not been made on effective utilization of the natural material Sudachi.

  Then, this invention is providing the composition using the natural raw material effectively, the pharmaceutical composition containing the said composition, health food-drinks, and a supplement.

  As a result of diligent studies to solve the above problems, the present inventor has found that dried sudachi peel and flesh squeezed koji powder have an effect of suppressing blood sugar elevation, improving blood lipid, and increasing survival rate in animal experiments. confirmed. The present invention has been completed based on these findings.

That is, the manufacturing method of the freeze-dried and powdered composition (mixture) is, pericarp Sudachi (Citrus sudachi Hort), pulp after窄汁, and at least one member selected from the group consisting of seeds - Including the steps of putting in a pot in the range of 40 to -25 ° C, 24-26 hours after adding the temperature gradient, taking out the pot in the range of 40 to 60 ° C, freeze-drying , and powdering, The composition contains at least one selected from the group consisting of hesperidin and flavonoids .

The freeze-dried / powdered composition of the present invention is manufactured by the above-described manufacturing method.

Further, the pharmaceutical composition , health food or supplement of the present invention contains the lyophilized / powdered composition described above, and is used for lowering blood glucose level, and the lyophilized / powdered composition has side effects. It is characterized by having a hypoglycemic action, a blood lipid improving action, and a survival rate improving action.

Here, the pharmaceutical composition , health food or supplement for lowering the blood glucose level is characterized in that the side effects are an increase in blood pressure and an increase in pulse.

In addition, the pharmaceutical composition, health food or supplement for lowering the blood sugar level is characterized in that the blood glucose lowering action suppresses an increase in blood sugar level due to daily and sugar load.

Here, the pharmaceutical composition, health food or supplement for lowering the blood sugar level is characterized in that the blood lipid improving action lowers the arteriosclerosis index.

  Moreover, the supplement of this invention contains the composition derived from the said Sudachi.

  In a preferred embodiment of the supplement of the present invention, the supplement is in the form of a tablet, capsule, liquid, powder or soft capsule.

  The composition derived from Sudachi of the present invention has an advantageous effect of having a hypoglycemic action. Moreover, since the material is a natural material, there is no risk of side effects on living organisms, and it has the effect of effectively lowering the blood sugar level. In addition, the composition derived from Sudachi of the present invention is advantageous in that it can provide a product derived from Sudachi that contains a compound that causes a decrease in blood glucose level in the human body that has developed diabetes and that reduces the risk of developing diabetes in the future. Has an effect.

  The composition of the present invention is obtained by freeze-drying and pulverizing at least one selected from the group consisting of pericarp (Citrus sudachi Hort) peel, pulp after squeezed juice, and seeds. In addition, the pharmaceutical composition of the present invention contains an effective amount of the above-mentioned Sudachi-derived composition for lowering blood glucose level or preventing arteriosclerosis. Furthermore, the health food or drink of the present invention contains the composition derived from Sudachi of the present invention. Here, the term “healthy food” or “healthy beverage” is not particularly limited, and broadly means a food or beverage containing the composition derived from Sudachi of the present invention. The composition derived from the sudachi of the present invention can be edible as it is, but can be edible by mixing with other natural materials as described later so that it can be edible naturally. Furthermore, the supplement of the present invention contains the composition derived from Sudachi of the present invention. Here, the term “supplement” is based on the DSHEA Act established in 1994 (DSHEA (Dietary Supplement Health and Education Act) is a law concerning supplements of the FDA established in 1994). As clearly defined as `` a product for nutritional supplements containing one or more nutritional components such as amino acids '', the shape is not a regular food form such as tablets, capsules, powders, soft capsules, liquids, etc. Means something.

<Method for preparing composition derived from sudachi>
First, a method for preparing a composition derived from Sudachi will be described. Sudachi fruits and the like have been conventionally edible, and the fruits are shipped through a process such as squeezing in an agricultural factory process. In the agricultural factory process, the remaining skin and fruit squeezed rice cake (including seeds) are usually discarded. However, in the present invention, the residual skin and fruit squeezed rice cake are effectively used. The fruit skin and / or the fruit squeezed koji are once stored frozen as necessary. Such peels and / or pulped lees are freeze-dried. In the present invention , the kettle is put in a range of -40 to -25 ° C, and the freeze-drying is carried out in a range of 40 to 60 ° C after 24 to 26 hours from putting the kettle in a temperature gradient. In addition, the viewpoint that the quality may not be stabilized if the skin and / or the fruit squeezed koji is cut before freeze drying, if necessary, and thawed to cut the raw material and then frozen rapidly. From this, the skin and / or the fruit squeeze may be pre-frozen. As a pre-freezing condition, put in a kettle at + 15 ° C to + 5 ° C and freeze at -25 ° C to -40 ° C. The prefreezing time is not particularly limited, but is preferably 15 to 17 hours from the viewpoint of leaving the active ingredient as much as possible. The temperature in the kettle was set to + 15 ° C to + 5 ° C from the viewpoint of leaving the active ingredient as much as possible. The prefreezing temperature was set to -25 ° C to -40 ° C because the active ingredient was From the viewpoint of leaving as much as possible. The powdering method is not particularly limited, and can be performed according to a known method such as pulverization.

In the present invention , the composition obtained by freeze-drying and pulverizing sudachi peels and / or pulped koji contains at least one selected from the group consisting of hesperidin and flavonoids. Among these ingredients, hesperidin is contained in the citrus bag part 50 times as much as the pulp, and in the streak part 300 times as much as the pulp. Hesperidin is contained, and it is considered that blood glucose level lowering action and arteriosclerosis preventing action are exhibited by the synergistic effect of hesperidin and other components.

<Effective amount (for blood glucose level lowering effect)>
The composition derived from Sudachi of the present invention is effective for lowering blood glucose level. The pharmaceutical composition for lowering blood glucose level of the present invention contains an effective amount of the composition derived from the above Sudachi in order to lower the blood glucose level. Here, the effective amount is preferably 3 to 20 g / day.

  The pharmaceutical composition for lowering blood glucose level according to the present invention comprises an effective amount of a composition derived from Sudachi, and is prepared in the form of an appropriate dosage form.

  The dose of the composition derived from Sudachi can be changed according to the pathology and severity of the subject patient, the dosage form, the selected route of administration, the number of administrations per day, and the like.

  The dose of the Sudachi-derived composition in the pharmaceutical composition for lowering blood glucose level of the present invention is an amount that is at least 320 mg / kg / day in rats in a lyophilized powder state, and the same amount or less in humans. It is preferable that

<Effective amount (for preventing arteriosclerosis)>
The composition derived from Sudachi of the present invention is also effective for preventing arteriosclerosis. The pharmaceutical composition for preventing arteriosclerosis of the present invention contains an effective amount of the composition derived from the sudachi in order to prevent arteriosclerosis. Here, the effective amount is preferably 3 to 20 g / day.

  The pharmaceutical composition for preventing arteriosclerosis according to the present invention contains an effective amount of a composition derived from Sudachi and is prepared in the form of an appropriate dosage form.

  The dose of the composition derived from Sudachi can be changed according to the pathology and severity of the subject patient, the dosage form, the selected route of administration, the number of administrations per day, and the like.

  The dose of the Sudachi-derived composition in the pharmaceutical composition for preventing arteriosclerosis of the present invention is an amount that is at least 320 mg / kg / day in rats in a lyophilized powder state, and the same amount or less in humans. It is preferable that

  Examples of the dosage form include oral preparations (tablets, capsules, coated tablets, granules, solutions, syrups), rectal suppositories, injections, and the like. The patient to be administered is often a chronic disease, and is preferably an oral preparation from the viewpoint of necessity for long-term administration and ease of continuous use.

  The dosage form may contain other conventional ingredients such as stable preservatives, sweeteners, colorants, fragrances and the like.

<Acute toxicity test>
Hesperidin is a component that is a natural material and is likely to be particularly problematic in terms of toxicity. According to the literature, it has been reported that no toxicity is shown in mice even when fed a diet containing 5% methyl hesperidin (Toxico Lett. 1993 Jul; 69 (1): 37-44).

<Healthy food and drink>
Next, the health food and drink of the present invention will be described. About 1 to 20 g of the powder of the Sudachi-derived composition obtained as described above can be dissolved and used in 20 to 200 ml of water. This can be taken 2-3 times a day.

  The health food containing the sudachi-derived composition can be used as a meal substitute or snack. In this case, the Sudachi-derived composition powder and at least one selected from the group consisting of rice flour, buckwheat flour, udon flour, wheat flour, and bread crumb can be prepared and used for ordinary cooking. Further, these may be processed into Western confectionery, Japanese confectionery, confectionery, udon, soba, pasta, Chinese soba, bread, etc. to make it easier to eat. These cooking methods follow conventional methods.

  The state of the health food may be solid (powder, granule), jelly, liquid, or suspension. The health food or health drink may contain various components contained in sweeteners, acidulants, vitamins, drinks, and the like.

  In the case of a health drink, it may further contain at least one selected from the group consisting of water, cocoa, coffee, tea, milk, liquor, fruit drink, and vegetable drink. When about 1 to 10 g of powder of the composition derived from sudachi obtained as described above is dissolved in 20 to 200 ml of water and edible, it can be further dissolved in water, cocoa or the like and drunk.

  In any case, the method for eating and drinking the health food and health drink of the present invention is free and not particularly limited. Therefore, the effect of the present invention can be expected as long as it is a food and drink method that utilizes food materials. Therefore, the embodiment of the invention and the eating and drinking methods described in the following examples are merely examples of the present invention used for explanation and should not be construed as limiting.

<Supplement>
Next, the supplement of this invention is demonstrated. The supplement of the present invention contains the sudachi-derived composition of the present invention described above. Supplements are especially useful for those who are busy and unable to eat a normal diet, who are hiking, cycling, running, competitors, etc. who need an instant high-energy snack packaged during exercise. It becomes supply. In the supplement of the present invention, regarding the composition derived from Sudachi, the above description can be applied as it is.

  In addition to the Sudachi-derived composition, the compounding ingredients to be blended in the supplement of the present invention are not particularly limited. Examples of such ingredients include wheat starch, rice starch, corn starch, potato starch, pregelatinized starch, partially pregelatinized starch, lactose, maltose, reduced lactose, reduced maltose, sorbitol, mannitol, erythritol, xylitol, sucrose, Glucose, guar gum, xanthan gum, sodium alginate, gum arabic, tragacanth gum, pullulan, agar, gelatin, soy dietary fiber, crystalline cellulose, methylcellulose, ethylcellulose, low substituted hydroxypropylcellulose, hydroxypropylmethylcellulose, hydroxypropylmethylcellulose phthalate, hydroxypropylmethylcellulose Acetate succinate, carboxymethyl ethyl cellulose, cellulose acetate phthalate, hydroxyethyl cellulose, hydroxypropyl Lustach, polyvinylpyrrolidone, carmellose, carmellose calcium, carmellose sodium, croscarmellose sodium, carboxymethyl starch sodium, hardened vegetable oil, vegetable oil powder, carnauba wax, cacao oil powder, sucrose fatty acid ester, stearic acid, magnesium stearate And commercially available products such as aluminum stearate, calcium stearate, silicon dioxide, talc, aluminum silicate, calcium hydrogen phosphate, and the like.

  Preferred embodiments of the supplement of the present invention are tablets, capsules, liquids, powders, and soft capsules. For example, the tablet may be compression-molded according to a conventional method by mixing the above-mentioned Sudachi-derived composition and, if necessary, the above-mentioned compounding ingredients, or the above-mentioned Sudachi-derived composition and, if necessary, the above-mentioned compounding ingredients. Can be made into granules by an appropriate method, and then compression molded according to a conventional method.

  Moreover, you may use a commercial confectionery coating material for the supplement of this invention. In the present invention, the confectionery coating material may be cocoa butter-based or a compound coating made of hardened vegetable oil. Examples of hardened vegetable oils used in the compound coating include cottonseed oil, coconut oil, soybean oil, palm oil, and peanut oil. These hardened vegetable oils are mixed with sugar and form the main component of the coating material. The formulated coating may be flavored with a peanut flavor, fruit flavor, chocolate flavor, vanilla flavor, coconut flavor, or other commercially available flavoring.

  The supplements according to the present invention can also be fortified by using proteins obtained from several edible protein sources, alone or in combination. Examples of protein sources include whey powder, locust bean flour, soybean lecithin, peanut flour, wheat protein (wheat malt, etc.) and caseinates (calcium caseinate, potassium caseinate, sodium caseinate, etc.).

  Examples of carbohydrate sources suitable for the supplement of the present invention include cereal syrup malted with corn, barley, brown rice, etc .; malt dextran; fructose; high fructose corn syrup; date palm paste; sucrose; red sugar and mixtures thereof. It is done. Examples of complex polysaccharide sources include those derived from cereals such as oats, rice, barley and corn.

  The supplement of the present invention may further contain fat. Fat is the source that provides the most energy per unit weight and provides approximately twice as much energy as protein or carbohydrate. In addition to confectionery coating materials, examples of fat sources used in the present invention include flavors such as chocolate, cocoa and coconut, and at least one partially hydrogenated vegetable oil (soybean oil, cottonseed oil, corn oil, Palm oil).

  In another preferred embodiment, the supplement is provided in the form of a beverage. Examples of beverages used in the present invention include, but are not limited to, water; fruit juices such as apple juice, orange juice, grapefruit juice, cranberry juice, pineapple juice, grape juice; and mixtures thereof. is not.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not intended to be interpreted as being limited to the following examples.

Example 1
<Preparation of dried sudachi peel and pulp squeezed>
Sudachi peels and flesh squeezed grapes that had been stored frozen were used for freeze-drying. Freeze-drying was carried out at -35 ° C for 24 hours, then taken out at + 45 ° C and pulverized to obtain a powder derived from Sudachi. Next, the components of the powdered sample were examined after freeze-drying the sudachi peel and the fruit squeezed strawberries. From the results of nutritional analysis of the powdered sample after freeze-drying the Sudachi peel and the fruit squeezed persimmon, the following components were contained in 100 g of the dried Sudachi pericarp and the fruit squeezed persimmon (see Table 1). In addition, in the column of the synephrine content in Table 1, “F. Kusu, K. Matsumoto, K. Takamura, Direct Separation and Determination of Synephrine Enantiomers by High-Performance liquid Chromatography with Electrochemical Detection, Chem. Pharm. Bull. , 43 (7), 1158-1161 (1995). "The value of synephrine content in 100 g of dried sudachi peel was described as a reference value.

(Example 2)
Next, using Zucker-fatty rat, the anti-diabetic effect of dried sudachi peel and pulp squeezed was examined. Here, Zucker fatty rat is a rat with obesity due to polyphagia and presenting hyperlipidemia, high insulin plasma, and high leptin plasma, and is widely used for research on abnormal sugar / lipid metabolism and insulin resistance associated with obesity. Has been.

  The 6-week-old Zucker-fatty rat was divided into two groups (n = 7): the control group, the dried sudachi peel / fruit pulp crumb powder group (320 mg / kg / day), Sudachi dry pericarp / fruit pulp squeezed powder water suspension (160 mg / ml) is orally administered once a day at a rate of 2 ml / kg using an oral sonde. In the control group, distilled water is administered in the same manner. Body weight is measured daily, blood pressure (maximum, minimum, heart rate) is measured every week, blood glucose level is measured every half month, and blood insulin concentration is measured every month. Blood lipids were administered on the 130th day after administration of dried sudachi peel and pulp squeezed.

  The change in body weight is shown in FIG. From these results, it was shown that administration of dried sudachi peel and pulp pomace does not decrease body weight.

  Changes in blood pressure and pulse are shown in FIGS. FIG. 2 shows changes in blood pressure due to administration of dried sudachi peel and pulp squeezed and FIG. 3 shows changes in pulse due to administration of dried sudachi peel and squeezed pulp. Sudachi dried pericarp and flesh squeezed koji contain synephrine with a molecular structure similar to that of epinephrine, which is a pressor substance, and it has been reported that this synephrine has an effect of increasing pulse and raising blood pressure. However, in this experiment, no significant increase in blood pressure or increase in pulse was observed due to the administration of dried sudachi peel or pulp squeezed.

  Changes in blood lipids are shown in FIGS. FIG. 4 shows changes in total cholesterol (A), HDL cholesterol level (B), and LDL cholesterol level (C) by administration of dried sudachi peel and pulp squeezed (blood sampling is 6 months after the start of the experiment). FIG. 5 shows changes in the arteriosclerosis index (blood sampling is 6 months after the start of the experiment) by administration of dried sudachi peel and pulp squeezed. As a result, the free fatty acid and HDL / LDL cholesterol levels were not changed by administration of dried sudachi peel / pulp extract (see FIG. 4). Moreover, a decrease in arteriosclerosis index [(total cholesterol value−HDL cholesterol value) ÷ HDL cholesterol value] was observed by the administration of dried sudachi peel and pulp squeeze (see FIG. 5).

The change in blood glucose level is shown in FIG. Figure 6 shows the change in blood glucose level by Sudachi dried peel-pulp pomace administration ^(# p <0.05). As a result, the blood glucose level increased with time in the control group, whereas the increase in blood glucose level was significantly suppressed in the Sudachi dry peel / pulp extract administration group.

  Changes in blood insulin concentration are shown in FIG. FIG. 7 shows changes in blood insulin concentration due to administration of dried sudachi peel and pulp squeezed. As a result, regarding the blood insulin concentration, there was no difference between the control group and the Sudachi dried peel / fruit pulp squeezed group.

  From the above results, it was observed that administration of dried sudachi peel / pulp pomace did not change blood insulin concentration or lipid metabolism, but significantly decreased blood glucose level.

  Next, from the results shown in FIG. 6, a hyperglycemia improving action (insulin resistance improving action) was observed by administration of dried sudachi peel and flesh squeezed koji. In order to further clarify this point, a glucose tolerance test was conducted. .

  Glucose tolerance test is a method in which an animal fasted overnight drinks a certain concentration of glucose, measures blood glucose level immediately before ingestion and after a certain period of time after ingestion, and analogizes the degree of diabetes from the transition pattern of the blood glucose level. It is. In particular, in a typical diabetic state, the blood glucose level is abnormally high 30 minutes and 1 hour after glucose intake, and a characteristic pattern that does not decrease easily after 180 hours at 180 mg / dl or more.

  Therefore, blood was collected after fasting the rats overnight, and the blood glucose level before the start of the experiment was measured and immediately 0.75 g / ml Glucose solution was orally administered at a rate of 4 ml / kg, 5 minutes after administration, 10 minutes, The blood glucose level was measured after 15 minutes, 30 minutes, 45 minutes, 1 hour, 1 hour and 30 minutes, 2 hours, 3 hours, 4 hours, 6 hours, and 8 hours. FIG. 8 shows the results of the glucose tolerance test by the administration of dried sudachi peel and pulp pomace.

  As a result, in the control group, the blood glucose level was already 130 mg / dl (normal value was 100 mg / dl or less) before taking glucose, and when glucose was ingested, the blood glucose level increased to 310 mg / dl. On the other hand, in the Sudachi dry peel / fruit pulp squeezed group, the blood sugar level at fasting was 90 mg / dl, and even when glucose was ingested, the maximum blood concentration was 218 mg / dl. In this way, the increase in blood sugar level due to sugar load was suppressed in rats fed dry sudachi peel and pulp squeezed so that intake of dry sudachi peel and pulp squeezed suppressed the increase in blood sugar level in diabetes This was confirmed with the results of FIG.

  Furthermore, when the above-mentioned Zucker-fatty rats were orally administered with sudachi dry peel and flesh squeezed at a rate of 320 mg / kg per day for one year, a significant improvement in survival rate was observed in the first year of administration. (See FIG. 9). From this, it was strongly suggested that dried sudachi peel and flesh squeezed koji have some effect of improving survival as well as preventing diabetes.

(Example 3)
<Evaluation of medicinal effects of dried sudachi peels and squeezed pulp using cultured cells>
Recent studies have pointed out that the presence of “oxidative stress” produced in vivo is greatly involved in the progress of aging. Therefore, we examined the protective effect of cultured two-component components (Hesperidin, Hesperetin) contained in dried sudachi peel and pulped sardine on antioxidant cells and cultured cells subjected to oxidative stress.

  Antioxidant activity was evaluated by the electron spin resonance (Electron Paramagnetic Resonance: EPR) method for the elimination activity against 1,1-Diphenyl-2-picrylhydrazyl (DPPH) having a relatively stable radical. Chem Pharm Bull (Tokyo). 52, 186-191, 2004.).

  As a result, although Hesperidin did not show DPPH scavenging activity, Hesperetin produced by hydrolysis in the digestive tract showed weak but antioxidant activity (1 DPPH radical was scavenged with 3 Hesperetin molecules).

Moreover, the protective effect with respect to the cultured cell which gave the oxidative stress was evaluated as follows. First, mesangial cells (1 × 10 ⁵ cells / well) isolated from the kidneys of Wistar male rats were cultured for 24 hours, then starved for 12 hours, and then 50 mM samples (Hesperidin, Hesperetin and Sudatitin) were added. . After 1 hour, add 300 mM hydrogen peroxide as a source of oxidative stress for cultured cells, incubate for 8 hours, and then measure leakage of lactate dehydrogenase (LDH) from the cells to examine the protective effect against oxidative stress did. FIG. 10 shows the effect of suppressing oxidative stress by Hesperidin and Hesperetin (p <0.01), and FIG. 11 shows the effect of suppressing oxidative stress by Sudatitin (p <0.001).

  As a result, both Hesperetin (see FIG. 10) and Sudatitin (see FIG. 11) significantly improved the damage of mesangial cells caused by hydrogen peroxide.

  Since the composition derived from Sudachi of the present invention has a hypoglycemic action, a blood lipid improving action, and a survival rate improving action without causing side effects on organisms, it can be applied in a wide range of fields.

FIG. 1 shows the change in body weight. FIG. 2 shows changes in blood pressure. FIG. 3 shows changes in pulse by administration of dried sudachi peel and pulp squeezed. FIG. 4 shows changes in total cholesterol (A), HDL cholesterol level (B), and LDL cholesterol level (C) due to administration of dried sudachi peel and pulp squeezed (blood sampling is 6 months after the start of the experiment). FIG. 5 shows changes in the arteriosclerosis index by administration of dried sudachi peel and pulp squeezed (blood collection is 6 months after the start of the experiment). Figure 6 shows the change in blood glucose level by Sudachi dried peel-pulp pomace administration ^(# p <0.05). FIG. 7 shows the change in blood insulin concentration due to administration of dried sudachi peel and pulp pomace. FIG. 8 shows the results of a glucose tolerance test by administration of dried sudachi peel and pulp pomace. FIG. 9 shows the change in survival rate (p <0.05) by administration of dried sudachi peel and pulp pomace. FIG. 10 shows the effect of suppressing oxidative stress (p <0.01) by Hesperidin and Hesperetin. FIG. 11 shows the oxidative stress suppression effect (p <0.001) by Sudatitin.

Claims (8)

A method for producing a freeze-dried / powdered composition derived from Sudachi (Citrus sudachi Hort),
At least one selected from the group consisting of the skin of sudachi, the pulp after squeezing, and the seeds is put into a kettle in the range of -40 to -25 ° C., and 24-26 hours after putting the kettle in a temperature gradient. , Including the step of lyophilizing and pulverizing in a range of 40-60 ° C.,
The method for producing a freeze-dried / powdered composition, wherein the composition contains at least one selected from the group consisting of hesperidin and flavonoids. A freeze-dried and powdered composition produced by the production method according to claim 1. A pharmaceutical composition, a health food or drink, or a supplement for lowering blood glucose level, comprising the freeze-dried / powdered composition according to claim 2,
  The lyophilized / powdered composition has a blood glucose lowering action, a blood lipid improving action, and a survival rate improving action without causing side effects, a pharmaceutical composition, a health food or drink, or supplement. The pharmaceutical composition, health food or drink or supplement according to claim 3, wherein the side effect is an increase in blood pressure or an increase in pulse. The pharmaceutical composition, health food or drink or supplement according to claim 3 or 4, wherein the blood glucose lowering action suppresses an increase in blood sugar level due to daily and sugar load. The pharmaceutical composition, health food or drink, or supplement according to any one of claims 3 to 5, wherein the blood lipid improving action lowers an arteriosclerosis index. The supplement for lowering | hanging the blood glucose level as described in any one of Claims 3-6. The supplement according to claim 7 , wherein the supplement is in the form of a tablet, capsule, liquid, powder, or soft capsule.

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