JP7320167B2 - Oral bacteriostatic composition - Google Patents

Description

The present invention relates to oral bacteriostatic compositions.

Saccharides have been widely used for a long time as taste-promoting ingredients in foods, particularly sweetening ingredients. In recent years, attention has been paid to the various functions of saccharides, and usage methods according to the functions have been sought.

For example, 1,5-anhydro-D-fructose is a sugar that can be produced by reacting α-1,4-glucan such as starch with α-1,4-glucan lyase. - Starch syrup products containing relatively high concentrations of fructose are manufactured and sold in Japan as food ingredients.

This 1,5-anhydro-D-fructose has antioxidant and bacteriostatic effects and is used for the purpose of maintaining the quality of processed foods (Non-Patent Document 1). In addition, health functions such as anti-inflammatory action, anti-allergic action, and platelet aggregation inhibitory action have been reported, and it is also used in foods for the purpose of maintaining health.

Japanese Journal of Applied Glycoscience, Vol. 1 (2011) No. 1 70-75

Thus, 1,5-anhydro-D-fructose, which has various functions, is one of the sugars whose usefulness is widely expected. As a typical example, in the application field related to improving the shelf life of processed foods (extending the expiry date), further improvement in bacteriostatic activity is required so that it can be applied to many processed foods.

In addition, although many chemically synthesized food additives such as glycine and acetic acid have been used to extend the shelf life of processed foods, some consumers are concerned about chemically synthesized products. With this in mind, many people choose processed foods that do not contain food additives.

On the other hand, 1,5-anhydro-D-fructose is a safe and secure natural material that can be produced by decomposing starch with edible seaweed enzymes. When used for improvement, the fact that it is not a chemically synthesized product but a natural material has great merit.

In addition, since 1,5-anhydro-D-fructose is a sugar, it only gives off a weak sweetness, and when added to processed foods, it has the excellent advantage of having almost no effect on the flavor of the foods. there is

However, in order to make it applicable to more processed foods, it is also the actual situation that a technique for enhancing the bacteriostatic activity is required.

Considering a little more medical and pharmaceutical cases, for example, there are many bacteria in the oral cavity, and 1,5-anhydro-D-fructose can be used for bacteriostasis.

That is, among oral bacteria, for example, Streptococcus mutans is known to cause caries and gingivalis is known to cause periodontal disease, and some of these oral bacteria may adversely affect human health. There is

Dental pastes, mouthwashes, etc. are sold to suppress these bacteria, but the active ingredients of these products are mainly chemically synthesized products, and it is necessary to spit them out after cleaning the oral cavity.

On the other hand, the need for oral care, including prevention of bad breath, is becoming more and more important for nursing care and etiquette. The demand for products is also expected to increase in the future.

In this regard, 1,5-anhydro-D-fructose is not only edible, but also has excellent bacteriostatic activity. If it becomes possible to further promote this, it is believed that it will greatly contribute to the development of the above-mentioned products.

Thus, there is a growing demand for a technology that can promote the bacteriostatic effect of 1,5-anhydro-D-fructose, regardless of whether it is food or pharmaceuticals.

The present invention has been made in view of such circumstances, and has a track record of being generally orally ingested, and has a taste and flavor of 1,5-anhydro-D-fructose with as little offensive taste and offensive odor as possible. To provide an oral bacteriostatic composition containing a substance capable of promoting the bacteriostatic effect of 1,5-anhydro-D-fructose without impairing

The present invention also provides the use of killed lactic acid bacteria powder for enhancing the bacteriostatic action of 1,5 -anhydro-D-fructose.

In order to solve the above conventional problems, the oral bacteriostatic composition according to the present invention contains (1) 1,5-anhydro-D-fructose and killed lactic acid bacteria powder.

Moreover, the oral bacteriostatic composition according to the present invention is also characterized in that the killed lactic acid bacteria powder is the killed powder of Enterococcus faecalis.

In addition, in the present invention, ( 2 ) when enhancing the bacteriostatic action of 1,5-anhydro-D-fructose present in food, killed Enterococcus faecalis powder is used in the food as a bacteriostatic action enhancer. I decided to

According to the oral bacteriostatic composition according to the present invention, since it contains 1,5-anhydro-D-fructose and killed lactic acid bacteria powder, it has a track record of being generally orally ingested. an oral bacteriostatic composition containing a substance capable of promoting the bacteriostatic effect of 1,5-anhydro-D-fructose, without impairing the taste and flavor of 1,5-anhydro-D-fructose. can provide things.

Further, if the killed lactic acid bacteria powder is the killed powder of Enterococcus faecalis, the effect of enhancing the bacteriostatic action of 1,5-anhydro-D-fructose can be obtained more steadily.

Further, according to the use according to the present invention, when enhancing the bacteriostatic action of 1,5-anhydro-D-fructose present in food, the dead bacteria powder of Enterococcus faecalis is added to the food as a bacteriostatic action enhancer. 1,5-anhydro-D-fructose has a track record of being generally orally ingested so far, and 1,5-anhydro-D- It can help the bacteriostatic effect of fructose. Moreover, it also has the effect that improvement of dementia can be expected.

The present invention provides 1,5-anhydro-D-fructose, which has a track record of being generally orally ingested, and which does not impair the taste and flavor of 1,5-anhydro-D-fructose with as little offensive taste and offensive odor as possible. An object of the present invention is to provide an oral bacteriostatic composition containing a substance capable of promoting the bacteriostatic effect of D-fructose. It was completed based on the knowledge that the bacteriostasis effect is synergistically enhanced by mixing the bacteria of this strain without stimulating the taste and aroma.

Specifically, the oral bacteriostatic composition according to the present embodiment contains 1,5-anhydro-D-fructose and killed lactic acid bacteria powder.

1,5-anhydro-D-fructose can be produced by degrading α-1,4-glucan such as starch with α-1,4-glucan lyase, but the present embodiment is limited to this production method. In the case of 1,5-anhydro-D-fructose only, or glucose and α-1,4-glucan other than 1,5-anhydro-D-fructose, α-1,4-glucan lyase Undecomposed substances and the like when decomposed may also be included. Also, 1,5-anhydro-D-fructose may be in a dry powder or liquid state.

On the other hand, the dead lactic acid bacteria powder is obtained by separating the cells of lactic acid bacteria from the fermented liquid after lactic acid fermentation and sterilizing them. You can use what you get from

Lactic acid bacteria is a general term for bacteria that produce lactic acid through metabolism, and includes the genus Lactobacillus, Enterococcus, Lactococcus, Pediococcus, Leuconostoc, Streptococcus, and Bifidobacterium. It is preferable that the lactic acid bacteria themselves have little offensive taste or aroma.

As described above, the type of lactic acid bacteria is not particularly limited in the preparation of the oral bacteriostatic composition according to the present embodiment. , Enterococcus faecalis EF-2001 strain is good.

The mixing ratio of 1,5-anhydro-D-fructose and the dead lactic acid bacteria powder is an amount equivalent to 10 billion to 7.5 trillion bacteria per 1 g of 1,5-anhydro-D-fructose. It is preferable to mix dry dead cell powder, preferably in an amount corresponding to 50 billion to 1 trillion cells, more preferably 200 billion to 700 billion cells. If the number is less than 10 billion, the effect of the lactic acid bacteria is difficult to exert, which is not preferable.

The oral bacteriostatic composition according to the present embodiment is not particularly limited as long as it is orally ingested by humans or non-human animals. It can also be understood as foods including sex foods, nutritional supplements, foods for specified health uses, and the like. In addition to the oral bacteriostatic composition according to the present embodiment, these drugs and foods may, of course, contain other compositions, food ingredients, and the like.

Examples of the use of the oral bacteriostatic composition itself according to the present embodiment, or when it is processed as a drug or food, include improving the shelf life of processed foods and pet foods, suppressing oral bacteria in pets and humans, and improving bowel movements in pets and humans. can be used for

Examples of processed foods include raw noodles, dried udon noodles, soba noodles, Chinese noodles, side dishes, sweets, breads, and bread fillings, but are not limited to these.

In addition, the oral bacteriostatic composition according to the present embodiment naturally has a bacteriostatic effect, but surprisingly, it is characteristic in that it can be expected to improve dementia in humans and non-human animals. is.
This point will be described later with reference to test results and the like.

In addition, the dosage form of the oral bacteriostatic composition, drug, and food according to the present embodiment is not particularly limited, and may be, for example, powder, tablet, granule, liquid, capsule, or syrup. It can be in any form, such as a paste-like mixture of 1,5-anhydro-D-fructose and dry powder of lactic acid bacteria.

Also, excipients and solvents are not limited at all, but examples of excipients include glucose, maltodextrin, starch syrup, starch, and lactose. Moreover, water, fruit juice, alcohol, milk, etc. can be illustrated as a solvent.

Hereinafter, the oral bacteriostatic composition and dementia-improving agent according to the present embodiment will be further described with reference to test results and the like.

[1. Offensive Taste/Odor Confirmation Test]
The characteristics of 1,5-anhydro-D-fructose as a bacteriostatic material include that it has a bacteriostatic effect, that it is a natural food material, and that it does not have a strong offensive taste or odor.

In other words, users of 1,5-anhydro-D-fructose, represented by food companies, do not have offensive taste or offensive odor, and are subject to the bacteriostatic effect. One of the reasons why it is used as a manufacturing raw material is that it does not spoil the flavor of foods.

Therefore, the agent to be mixed with 1,5-anhydro-D-fructose should be a natural food material with bacteriostatic activity, and should have a strong taste when mixed with 1,5-anhydro-D-fructose. Absence of offensive taste and offensive odor is required.

Therefore, in this confirmation test, when enhancing the bacteriostatic action of 1,5-anhydro-D-fructose, the oral bacteriostatic agent according to the present embodiment using killed powder of Enterococcus faecalis EF-2001 strain as a bacteriostatic action enhancer. A fungal composition A1 was prepared, and sensory evaluations of offensive taste and offensive odor were carried out by three panelists.

In addition, brewed vinegar, citric acid fermentation liquid, wasabi, and garlic were selected as food materials known to have a bacteriostatic effect, and these were mixed with 1,5-anhydro-D-fructose to prepare a A sensory test was also conducted on the comparative compositions X1 to X4 as a comparative control.

In this test, Anhydroose (registered trademark) manufactured by Sanus Co., Ltd. was used as 1,5-anhydro-D-fructose. Anhydroose contains 1,5-anhydro-D-fructose at 27.0-30.0 wt% (e.g., 28.5 wt%), starch syrup at 41.5-44.5 wt% (e.g., 43.0 wt%), and water at 27.0-30.0 wt% in the product. % (for example, 28.5 wt%).

An oral bacteriostatic composition obtained by adding various food components to this 1,5-anhydro-D-fructose formulation (anhydrose), adding 2 parts by weight of various food components to 8 parts by weight of anhydrose, and mixing them. The product A1 and the comparative compositions X1 to X4 were sensory evaluated for offensive taste and offensive odor. Table 1 shows the results.

As can be seen from Table 1, as a result of this test, the comparative compositions X1 to X4 using brewed vinegar, citric acid fermented liquid, wasabi, and garlic had an offensive taste and odor.

On the other hand, the oral bacteriostatic composition A1 using Enterococcus faecalis EF-2001 strain did not give any offensive taste or offensive odor.

From these results, when enhancing the bacteriostatic action of 1,5-anhydro-D-fructose, the use of dead lactic acid bacterium powder, particularly Enterococcus faecalis powder, as a bacteriostatic action enhancer can be effective in this implementation. It is suggested that the bacteriostatic effect of 1,5-anhydro-D-fructose can be promoted without causing offensive taste and odor as much as possible even when the oral bacteriostatic composition according to the form itself or when processed as a drug or food. was done.

[2. Bacteriostatic effect confirmation test]
Next, in order to evaluate the bacteriostatic activity of the mixed preparation of 1,5-anhydro-D-fructose and lactic acid bacteria against bacteria, the samples shown in Table 2 were used, and the bacteriostatic activity against Bacillus subtilis was evaluated.

Specifically, 40 μL of the sample was added to 2 ml of Nutrient broth medium (containing 1% glucose, pH 6.0), and 20 μL of Bacillus subtilis preculture was added to adjust the bacterial count concentration to 100 per 1 ml.

After static culture of this at 35° C. for 20 hours, the number of bacteria was measured. The number of bacteria was determined from the number of colonies formed by placing the culture solution on a dry film (Petrifilm (registered trademark) manufactured by 3M) and culturing.

Table 3 shows the results of the tests conducted in this manner.

As can be seen from Table 3, under these conditions, anhydrose and lactic acid bacteria powder alone did not exhibit a bacteriostatic effect, but when anhydrose and lactic acid bacteria powder were mixed, a strong bacteriostatic activity was observed. Mixing 5-anhydro-D-fructose and lactic acid bacteria synergistically enhanced the bacteriostatic activity.

[3. Preparation of dementia improving agent]
Next, a dementia-improving agent was prepared as one aspect of a drug or food containing the oral bacteriostatic composition according to the present embodiment. Specifically, it is a paste-like dementia-improving agent filled in an aluminum pouch at a rate of 3 g per package.

Each raw material shown in Table 4 was put into a stainless steel mixing tank and stirred while being heated to 30 to 35° C. to obtain a paste-like mixture.

Next, the mixture was supplied to an aluminum pouch filling machine and divided into 3 g portions, and the obtained packages were sterilized in a hot water bath at 80 to 85° C. for 35 minutes or more to obtain dementia improving agent B1.

[4. Dementia improvement effect confirmation test]
Next, [3. Preparation of dementia-improving agent] was used to confirm the dementia-improving effect using the dementia-improving agent B1 according to the present embodiment.

To 18 dementia patients in a facility for the elderly in Toyama Prefecture, one packet of dementia-improving agent B1 was orally administered once a day, and the Hasegawa score (dementia diagnosis) and patient condition were monitored over time Examined. Table 5 shows the results.

As can be seen from Table 5, oral ingestion of dementia-improving agent B1 increased the Hasegawa score with the lapse of time, demonstrating an improvement effect on dementia. Specifically, the average value of the Hasegawa score before the start of intake was 11.6, which is judged as "slightly advanced dementia", but after 6 months of intake, it was judged as "moderate dementia". improved to 15.1.

Thus, according to the dementia-improving agent B1 according to the present embodiment, by performing regular oral ingestion, three mild dementia patients (MCI subjects) returned to a normal state after 6 months of ingestion. shown to recover to

In addition, Table 6 shows a summary of stool conditions and peripheral symptoms after 6 months of ingestion.

As can be seen from Table 6, most of the subjects who took the dementia-improving agent B1 according to the present embodiment showed an improvement effect on bowel movement and fecal odor, and further improved peripheral symptoms such as communication ability.

In this study, dementia-improving agent B1 was orally administered to elderly patients with dementia every day for 6 months. Some subjects did so.

As described above, the oral bacteriostatic composition according to the present embodiment contains 1,5-anhydro-D-fructose and killed lactic acid bacteria powder, and thus has been generally orally ingested. A substance that has a proven track record and can promote the bacteriostatic effect of 1,5-anhydro-D-fructose with as little offensive taste and odor as possible without impairing the taste and flavor of 1,5-anhydro-D-fructose. Formulated oral bacteriostatic compositions can be provided.

Finally, the description of each embodiment mentioned above is an example of this invention, and this invention is not limited to the above-mentioned embodiment. Therefore, it goes without saying that various modifications other than the above-described embodiments can be made in accordance with the design and the like within the scope not departing from the technical idea of the present invention.

Claims (2)

In an oral bacteriostatic composition containing 1,5-anhydro-D-fructose and killed lactic acid bacteria powder,
An oral bacteriostatic composition, wherein the killed lactic acid bacteria powder is the killed powder of Enterococcus faecalis . Use of killed powder of Enterococcus faecalis in food as a bacteriostatic action enhancer to enhance the bacteriostatic action of 1,5-anhydro-D-fructose present in said food.