JP2019135212A - Fat production promoter - Google Patents

Abstract

To search for a material that promotes fat production of an adipocyte and provide the material as an external composition for improving a texture and appearance of a lip.SOLUTION: The present invention provides a fat production promoter containing Acer palmatum extract as an active ingredient and an external composition for lips containing the production promoter. The fat production promoter promotes fat production of an adipocyte and can be used, therefore, as the external composition for lips for improving lip textures and appearances, in the fields of pharmaceuticals, quasi drugs, cosmetic preparations.SELECTED DRAWING: None

Description

  The present invention relates to a fat production promoter and a composition for external use for lips containing the production promoter.

  Lips are parts that greatly affect the impression of the face, and the appearance of healthy lips with a plump and three-dimensional appearance is required. The skin of the lips is histologically different from the skin of other places, and since the stratum corneum is thin and there is little sebum, it is easily affected by physical, chemical and environmental factors in the skin. The three-dimensional appearance of the lips is due to the fat mass of the subcutaneous tissue, and a decrease in fat cell fat production due to aging or external stimulation can cause the lips to lose their plump and three-dimensional appearance ( Non-patent documents 1, 2).

  Many studies have been made on techniques for improving the texture and shape of the lips. Most lip treatment products currently on the market are composed of moisturizing oils or waxes that limit the amount of moisture dissipated from the lips (Patent Documents 1 and 2). These products are glossy with oil and wax to produce healthy lips that are visually three-dimensional, and are not a fundamental solution to improve the texture and shape of the lips. In terms of shape improvement, there are surgical techniques such as fat injection into the subcutaneous tissue and collagen injection into the dermis. However, there is a problem that physical damage and cost for performing a surgical procedure are high (Patent Document 3).

  Acer palmatum is a plant belonging to the genus Aceraceae, and the extract of Acer palmatum has so far been known to have medicinal effects such as an active oxygen scavenging action, a collagen production promoting action, an anti-inflammatory action, and a serine palmitoyltransferase mRNA expression promoting action. (Patent Documents 4 and 5). However, nothing has been known about the fat production promoting effect on fat cells.

JP 2010-265230 A JP2007-277151 Special table 2008-542252 JP 2003-1113068 JP2011-68594A

Rohrich RJ et al, Last Reconstr Surg. , 124 (1), 266-271 (2009) Sforza C et al., Forensic Sci Int. , 200 (1-3), 182. e1-7 (2010)

  An object of the present invention is to search for a substance that promotes the production of fat and provide it as a composition for external use for lips for improving the texture and shape of the lips.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that the extract of Japanese maple has the effect of promoting the production of fat in fat cells, and have completed the present invention.

That is, the present invention includes the following inventions.
(1) A fat production promoter comprising an extract of Acer palmatum.
(2) An external composition for lips comprising the fat production promoter according to (1).

  The fat production promoter of the present invention can be used as an external composition for lips for improving the texture and appearance of lips by promoting the production of fat by fat cells.

The present invention will be described in detail below.
The fat production promoter of the present invention is characterized by containing an extract of Acer palmatum. Acer palmatum (scientific name: Acer palmatum) is a deciduous tree belonging to the genus Maple. It is also called Maple or Takamomi Momiji and is distributed in Fukushima and Honshu, west of Fukui, Shikoku, Kyushu, southern Korea, and eastern China.

  In the present invention, the extract of Acer palmatum refers to an extract of the whole plant or a part of a plant such as a flower, stem, leaf, branch, branch, fruit, root, seed, bark, or a mixture thereof. Leaf extract is preferred. In addition, these plants may be used as they are for extraction, or may be subjected to treatments such as drying, pulverization and shredding.

  The extraction method is not particularly limited, and can be performed by stirring or column extraction using water or hot water, or a mixed solvent of water and an organic solvent. As the organic solvent, alcohols, ethers, esters, and the like can be used, but water-soluble organic solvents such as ethanol, methanol, and acetone are preferable, and one or more of these may be used. Particularly preferable extraction solvents include water or water-ethanol mixed polar solvents. The amount of the solvent used is not particularly limited. For example, it may be 10 times or more, preferably 20 times or more of the leaf of the white maple (dry weight), but it may be concentrated or isolated after extraction. For convenience of operation, it is preferably 100 times or less. Moreover, although extraction temperature and time depend on the kind of solvent to be used, for example, it is 10-100 degreeC, Preferably it is 30-90 degreeC, 30 minutes-24 hours, Preferably it can illustrate 1 to 10 hours. In addition, the extract may be used as an extracted solution, but if necessary, within a range that does not affect the effect, concentration (concentration by reduced pressure concentration, membrane concentration, etc.), dilution, filtration, activated carbon, etc. You may use it, after processing, such as decoloring, deodorizing, and ethanol precipitation. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.

  “Promoting fat production” in the present invention refers to promoting fat production by fat cells at the living body level or at the culture level.

  The fat production promoter of the present invention can be applied to all mammals as long as they have the same characteristics with respect to the fat production mechanism of adipocytes. For example, the effect can be exerted on adipocytes of mammals such as humans, monkeys, mice, rats, guinea pigs, hamsters, rabbits, cats, dogs, horses, cows, sheep, goats and pigs.

  When the fat production promoter of the present invention is administered in vivo, it can be administered as it is, but it is provided by blending it with a composition for external use with appropriate additives within a range not impairing the effects of the present invention. It is preferable. The skin external composition of the present invention includes pharmaceuticals, quasi drugs, cosmetics and the like. Preferably, the composition for external use for lips is good.

  Since the fat production promoter of the present invention can promote the production of adipocytes, the composition for external use for lips containing the substance is effective for improving the texture and appearance of the lips.

  When blending the fat production promoter of the present invention in a pharmaceutical product, it should be mixed with pharmacologically and pharmaceutically acceptable additives and formulated into various preparations suitable for application to the affected area. Can do. Pharmacologically and pharmaceutically acceptable additives include excipients, thickeners, tonicity agents, pH regulators, stabilizers, preservatives, preservatives depending on the dosage form and application. , Dispersants, emulsifiers, gelling agents, pigments, fragrances and the like can be used. A form suitable for the pharmaceutical product of the present invention is an external preparation, and examples thereof include an ointment, a cream, a gel, a liquid, and a patch. The ointment refers to a homogeneous semi-solid external preparation, and includes an oily ointment, an emulsion ointment, and a water-soluble ointment. The gel is an external preparation in which a water-insoluble component hydrate compound is suspended in an aqueous liquid. The liquid preparation refers to a liquid external preparation and includes lotions, suspensions, emulsions, liniments and the like.

  When the fat production promoter of the present invention is blended in an external composition, the dosage form is an aqueous solution system, a solubilization system, an emulsification system, a powder system, a powder dispersion system, an oil liquid system, a gel system, an ointment system, an aerosol. Any of a system, a water-oil two-layer system, or a water-oil-powder three-layer system may be used. In addition, the quasi-drug and cosmetics are selected from various ingredients, additives, bases, and the like that are usually used in a composition for external use for skin, together with a fat production promoter, It can be produced according to techniques known in the art. The form may be liquid, emulsion, cream, gel, paste, spray or the like. Examples of the components include oils and fats (olive oil, palm oil, evening primrose oil, jojoba oil, castor oil, hydrogenated castor oil, etc.), waxes (lanolin, beeswax, carnauba wax, etc.), hydrocarbons (liquid paraffin, squalene, Squalane, petrolatum, etc.), fatty acids (lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid etc.), higher alcohols (myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol etc.), esters (myristin) Isopropyl acid, isopropyl palmitate, cetyl octanoate, glyceryl trioctanoate, octyldodecyl myristate, octyl stearate, stearyl stearate), organic acids (citric acid, lactic acid, α-hydroxyacetic acid, pyrrolidone Bonic acid, etc.), sugars (maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, etc.), proteins and protein hydrolysates, amino acids and salts thereof, vitamins, plant / animal extract components, various interfaces Examples include activators, humectants, ultraviolet absorbers, antioxidants, stabilizers, preservatives, bactericides, and fragrances.

  The content of the fat production promoter in the composition for external use for lips of the present invention is not particularly limited as long as it is an amount capable of exerting a fat production promoting action from a fat cell. For example, the dry solid weight of the yellow maple extract is 0. 0.0001 to 10% by weight is preferable, and 0.0001 to 1% by weight is more preferable. The above amounts are merely examples, and may be appropriately set and adjusted in consideration of the type and form of the composition, the general usage amount, efficacy / effect, cost, and the like.

Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these.
(Production Example 1) Preparation of Acer palmatum hot water extract 500 mL of purified water was added to 25 g of dried leaves of Acer palmatum, extracted at 90-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized. 3.7 g of Acer palmatum hot water extract was obtained.

(Production Example 2) Preparation of 50% ethanol extract of Acer palmatum 500mL of 50 (v / v)% ethanol aqueous solution was added to 25g of dried leaves of Acer palmatum, extracted at room temperature for 3 days, filtered, and the filtrate was concentrated to dryness. Solidify to obtain 1.5 g of Acer palmatum 50% ethanol extract.

(Production Example 3) Preparation of Acer palmatum ethanol extract 500 mL of ethanol was added to 25 g of dried leaves of Acer palmatum, extracted at room temperature for 3 days, filtered, and the filtrate was concentrated to dryness. 0.1 g was obtained.

(Experimental example) Evaluation of fat production promoting effect in adipocytes 1 × 10 ⁵ human subcutaneous adipose tissue-derived stem cells (hADSC) were seeded in each well of a 24-well plate and cultured. After the culture, the medium was replaced with a differentiation induction medium (DMEM medium containing 1 μM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, 0.2 mM indomethacin, 10 μg / mL insulin, 33 μM biotin, 10% fetal bovine serum), and differentiation. Induction started. After differentiation into adipocytes, the medium was replaced with a mature medium (10 μg / mL insulin, 33 μM biotin, DMEM medium containing 10% fetal bovine serum) supplemented with samples, and further cultured for 5 days. After completion of the culture, the number of cells was measured, and the cells were stained with Oil Red O to stain intracellular fat. Thereafter, the stained fat was extracted with isopropanol, and the amount of intracellular fat was measured by measuring absorbance. The amount of intracellular fat production per cell was calculated from the number of cells measured in advance.

  The results of examining the fat production promoting effect are shown in Table 1. Japanese maple hot water, 50% ethanol and ethanol extract showed an excellent effect of promoting intracellular fat production.

(Prescription example) Product formulation example According to the formulation example shown below, the lip essence, lip balm, and liquid lip balm containing the extract of Acer palmatum produced in Production Examples 1 to 3 were prepared by a conventional method.

Formulation Example 1 Lip Essence Component Content (wt%)
1. Glycerin 5.0
2. 1,3-butylene glycol 5.0
3. Purified water remaining amount 4. Carboxymethylcellulose 0.5
5. Hydroxypropyl methylcellulose 0.5
6). Potassium hydroxide 0.5
7). Sorbitan sesquioleate 0.5
8). Monooleic acid polyoxyethylene sorbitan (20E.O.) 1.5
9. Liquid paraffin 5.0
10. Methyl paraoxybenzoate 0.3
11. Phenoxyethanol 0.01
12 Japanese maple extract of Production Examples 1 to 3 0.3

Formulation Example 2 Lip Balm Ingredient Content (wt%)
1. Polyethylene wax 1.5
2. Paraffin wax 9.0
3. Microcrystalline wax 3.0
4). Carnauba Arrow 3.0
5. Dextrin palmitate 0.25
6). Liquid paraffin 10.0
7). 7. Polyglyceryl-2 triisostearate remaining amount Acer palmatum extract of Production Examples 1 to 3 0.05

Formulation Example 3 Liquid Lip Balm Ingredient Content (wt%)
1. Polyethylene wax 2.0
2. Microcrystalline wax 5.0
3. Carnauba 0.5
4). Inulin stearate 0.1
5. Dextrin myristate 0.05
6). Heavy liquid isoparaffin 35.0
7). Diisostearyl malate 10.0
8). 8. Polyglyceryl-2 triisostearate remaining amount Japanese maple extract of Production Examples 1 to 3 0.1

  INDUSTRIAL APPLICABILITY The present invention can be used in the field of manufacturing pharmaceuticals, quasi drugs, and cosmetics for the purpose of improving the texture and appearance of lips.

Claims (2)

A fat production promoter comprising an extract of Acer palmatum. A composition for external use for lips comprising the fat production promoter according to claim 1.