JP2019118321A - Eggshell membrane-containing powder and/or hydrolyzed eggshell membrane component-containing jelly food product - Google Patents

Abstract

To provide a jelly food product for intestinal flora improvement function and for promoting type III collagen formation.SOLUTION: Provided is a jelly food product for promoting type III collagen formation, containing, as an active ingredient, an eggshell membrane-containing powder and/or a hydrolyzed eggshell membrane component.EFFECT: The jelly food product has a function of promoting type III collagen formation resulting from promotion of expression of type III collagen gene and an intestinal flora improvement function, and is an excellent functional jelly food product that is delicious and can easily be eaten.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a jelly food for improving intestinal flora and promoting type III collagen formation, which contains eggshell membrane-containing powder as an active ingredient. The present invention also relates to a jelly food for promoting type III collagen formation, which contains a hydrolyzed eggshell membrane component as an active ingredient. Furthermore, the present invention relates to a jelly food for improving intestinal flora and promoting type III collagen formation, which comprises an eggshell membrane-containing powder and a hydrolyzed eggshell membrane component as active ingredients.

  The eggshell membrane is a membrane inside the eggshell of avian egg such as a chicken egg and consists of 2 sheets inside and outside, the outer shell membrane is in close contact with the inner surface of the eggshell, and the inner shell is wrapped in egg white and has antibacterial properties , Protecting developing embryos from infection. Eggshell membrane has a network-like structure composed of type I, type V and type X collagen, glucosamine, desmosine and tough fibrous proteins mainly composed of hyaluronic acid, and these proteins contain cysteine Are relatively stable to acids, alkalis, and proteases, and insoluble in water. It was not used as a waste in the food industry before, but its development as a cosmetic and a nutritional agent has been promoted and put into practical use after its effect to promote skin regeneration and skin-care effect have been discovered.

  For example, in order to increase the efficiency of digestion and absorption of eggshell membrane components, eggshell membrane powder is pulverized using a jet mill so that the volume average particle size is 6 μm or less or the volume maximum particle size is 20 μm (800 mesh) or less. It has been proposed to powderize (patent document 1). In addition, in order to make insoluble eggshell membrane soluble in water, hydrolyzing hydrolyzed eggshell membrane obtained by hydrolyzing protein is dried and powdered for improvement of handling property and storage stability (Patent Document 2), and alkalinity It is proposed to increase the storage stability and safety by setting the pH of the hydrolyzed eggshell membrane powder to 3 to 6 (Patent Document 3).

  On the other hand, jelly foods refer to foods of various variations, and foods having a relatively wide range of properties from those having a moist "torro" texture to those that are hard enough to be able to retain a specific shape It is included. For example, meat and animal juices rich in collagen rich in meat are cooled down and boiled down by the action of gelatin, or sweetness such as sugar is added to juice or wine, and a gelling agent is added. A variety of food materials are used as the raw materials, and jelly foods having various properties, taste, and texture are produced.

JP, 2013-216652, A Patent No. 5179847 specification JP, 2016-98206, A

  The present invention is a jelly food which can easily eat a necessary amount anywhere, and has an object to provide a jelly food having an intestinal flora improving function in addition to the function of promoting the formation of type III collagen. . An object of the present invention is to provide a jelly food by promoting the expression of type III collagen gene in cells by the function of promoting the generation of type III collagen.

  Eggshell membrane or hydrolysed eggshell membrane is not only for skin softening, skin regeneration, hair growth promotion, hair loss prevention, etc., but also for daily health promotion and health maintenance such as elimination of stiff shoulder, enhancement of appetite, fatigue recovery, etc. It is considered to be effective. While the hydrolyzed eggshell membrane is almost 100% digested and absorbed in the blood, the eggshell membrane-containing powder and fine powder remain in the intestine without digestion and absorption of about 54%. , It was found that the remaining components prevent the growth of bad bacteria among enteric bacteria and improve enteric flora.

  In addition, since the eggshell membrane-containing powder or fine powder is digested and absorbed by about 46%, it can also promote the expression of the type III collagen gene in cells. The present inventors have found that the formation promoting function of type III collagen of the eggshell membrane-containing component is to promote expression of type III collagen gene in cells. The eggshell membrane-containing powder or fine powder simultaneously brings about the health and cosmetically required effects of the improvement function of intestinal flora and the generation function of type III collagen. Moreover, since it adds to jelly food, it can be used as health and beauty jelly food in combination with other health components and beauty components in jelly food, and can be eaten effectively and easily.

  In addition, since the hydrolyzed eggshell membrane component is 100% digested and absorbed, it is superior to the eggshell membrane-containing powder in the function of promoting the formation of type III collagen. And, by combining the eggshell membrane-containing powder or fine powder with the hydrolyzed eggshell membrane component, the intestinal flora improving function and the type III collagen generation promoting function act synergistically to improve the intestinal flora. In addition to improving immune function and preventing adult diseases such as cancer, strengthening anti-aging effect is enhanced by strengthening the tissue of blood vessels, lungs, liver and skin by promoting the generation of type III collagen, and the health condition is further improved and / or We came to complete jelly food that can be maintained well.

The present invention is composed of the following technical matters.
(1) A jelly food for promoting type III collagen formation, which comprises an eggshell membrane-containing powder and / or a hydrolyzed eggshell membrane component as an active ingredient.
(2) A jelly food for promoting type III collagen formation as described in the above (1), wherein an eggshell membrane-containing powder is added as an active ingredient to improve intestinal flora.
(3) The formation of type III collagen according to the above (1) or (2), wherein the eggshell membrane-containing powder is an eggshell membrane-containing fine powder having a volume average particle diameter of 6 μm or less or a volume maximum particle diameter of 20 μm or less Jelly food.
(4) The jelly food for promoting type III collagen formation according to the above (1), wherein the hydrolyzed eggshell membrane component is a hydrolyzed eggshell membrane powder.
(5) Type III collagen according to the above (1), wherein for the improvement of intestinal flora is added when 10 to 90 parts by weight of eggshell membrane-containing powder and 90 to 10 parts by weight of hydrolyzed eggshell membrane component are contained as active ingredients Jelly food for promoting production.
(6) The jelly food for promoting type III collagen formation according to any one of the above (1) to (5), wherein promotion of type III collagen formation promotes expression of a type III collagen gene in cells.
(7) Intestinal flora improvement, in the intestine, the component which is not absorbed and remains in the intestine increases the diversity of intestinal bacteria and decreases the number of pathogenic bacteria, as described above (2) Or the jelly food for promoting type III collagen formation according to (5).
(8) The jelly food for promoting type III collagen formation according to any one of the above (1) to (7), which is individually packaged in a smart pack package or a stick-like package container.

  Hundreds of intestinal bacteria inhabit in a well-balanced manner in the human intestine, and a good ecosystem, bad bacteria, and opportunistic bacteria balance each other in the intestine, a type of ecosystem (Intestinal flora) is formed. When the balance of the intestinal flora is broken, harmful substances are accumulated in the intestine due to the increase of bad bacteria and the like, and constipation, diarrhea symptoms, deterioration of the immune function as well as harmful substances absorbed in the blood, It is known to strain the heart, liver and kidneys, promote aging and also cause cancer.

The eggshell membrane-containing fine powder remains in the intestine without digestion and absorption for about 54% in the experiment using rats, and the remaining components prevent the growth of the bad bacteria of the enteric bacteria, and the enteric flora It was found to improve.
In addition, about 46% of the eggshell membrane-containing fine powder is digested and absorbed, about 100% of the hydrolyzed eggshell membrane component is digested and absorbed, and the expression of type III collagen gene is promoted by the eggshell membrane component taken into blood. In addition to promoting the formation of type III collagen in the skin, even blood vessels can promote the generation of type III collagen to increase the elasticity of the entire blood vessel.

Furthermore, when the eggshell membrane-containing powder or fine powder and the hydrolyzed eggshell membrane component are used in combination, the function to improve intestinal flora possessed by the eggshell membrane-containing powder or fine powder and the function to promote the formation of type III collagen and the hydrolysis eggshell membrane component have By acting synergistically with the type III collagen production promoting function, the skin-beautifying effect and anti-aging effect are enhanced by promoting the production of type III collagen in blood vessels, lungs, liver, skin, etc., and improving intestinal flora, Health conditions can be improved and / or well maintained.
Furthermore, if these active ingredients are individually packaged in a smart pack packaging or stick-like packaging container, it becomes a jelly food which can eat easily and easily the necessary amount, and provides excellent functional jelly food along with other ingredients in jelly food. be able to.

Results of Bacterial Metagenomics Analysis after Ingesting Eggshell Membrane-Containing Fine Powdered Supplements to DSS-Induced Colitis Mice: Enteric Bacterial Profile Results of Bacterial Metagenomics Analysis After Ingesting Eggshell Membrane-Containing Fine Powdered Supplements to DSS-Induced Colitis Mice: Abundance of Enteric Bacterial Species Results of bacterial metagenomic analysis of mice challenged with colitis with DSS after ingestion of eggshell membrane-containing fine powder supplementation: prevalence of enterobacteria The result after 17 days by the oral intake experiment of the hydrolysis eggshell membrane powder supplement using a mouse. The expression level in skin of a type III collagen gene by real time PCR is shown.

  Hundreds of enteric bacteria are inhabited in a balanced manner in human intestine in a balance of about 100 trillion or more. For example, useful bacteria such as lactic acid bacteria and bifidobacteria (good bacteria) such as E. coli and clostridial bacteria Harmful bacteria (bad bacteria) and opportunistic bacteria form a kind of ecosystem (gut flora) while maintaining balance with each other in the intestine. If the balance of the intestinal flora breaks down due to aging, stress, diseases, environmental changes, or changes in the diet, harmful substances are accumulated in the intestine due to an increase in bad bacteria etc., resulting in constipation, diarrhea, immune function In addition to lowering, it is known that harmful substances absorbed in the blood burden the heart, liver and kidneys and cause various diseases.

  The jelly for improving intestinal flora and promoting type III collagen formation according to the present invention comprises an eggshell membrane-containing powder as an active ingredient, and this active ingredient is an ingested eggshell membrane-containing fine powder in an experiment using a rat. 46% of it was digested and absorbed, the remainder was not absorbed and remained in the intestine. It is demonstrated in Example 1 that this remaining component can increase intestinal bacterial diversity and reduce the number of pathogenic bacteria such as E. coli.

As the eggshell membrane used in the present invention, any membrane can be used as long as it is inside the eggshells of all terrestrial egg live animals, particularly avian eggs. In particular, eggshell membranes of hen eggs are preferable in terms of availability and cost.
The eggshell membrane-containing powder used in the present invention is not particularly limited as long as it is a powder containing at least an eggshell membrane, and the powder refers to any powder regardless of the particle size. The raw material in a state in which the eggshell membrane is attached to the exfoliated eggshell membrane or eggshell may be powdered by any known method. Alternatively, a commercially available eggshell membrane-containing powder may be used.

  The eggshell membrane-containing fine powder to be used in the present invention refers to powder having a volume maximum particle size and / or a volume average particle size smaller than about 100 μm. In the present specification, “volume average particle diameter” and “volume maximum particle diameter” of powder or fine powder mean a value measured using a laser diffraction type particle size distribution analyzer (manufactured by Seishin Co., Ltd., LMS-30). Do. When measuring the particle size, a measurement sample in which eggshell membrane-containing fine powder is dispersed in water using a surfactant is used. Moreover, "volume average particle diameter" means the particle diameter in 50% of the cumulative value from the small particle diameter side in particle size distribution.

  As the eggshell membrane-containing fine powder to be used in the present invention, one having a volume average particle size of 6 μm or less, or a volume maximum particle size of 20 μm or less is particularly preferable from the viewpoint of digestive and absorption efficiency. It is preferable to manufacture eggshell membrane containing raw materials, such as a commercially available eggshell membrane containing powder, by a pulverization process using a jet mill in which they collide with each other in gas.

  The hydrolyzed eggshell membrane component used in the present invention is a solution having a protein degrading action such as an aqueous acid solution, an alkaline aqueous solution, an alkaline organic solvent solution, and a proteinase-containing solution, as the eggshell membrane or the aforementioned eggshell membrane-containing powder It is obtained by hydrolysis in vacuo. Although it can also be used as an aqueous solution or an extract, it is preferably in the form of a powder obtained by a drying means such as spray drying or lyophilization because of handleability and storage stability. As the hydrolyzed eggshell membrane powder, in particular, powders having a pH of 3 to 6 are preferable from the viewpoint of storage stability and safety.

  The human skin is composed of an epithelial layer on the surface and a connective tissue-like dermal layer below it, and the protein constituting the dermal layer is mainly collagen, and is also composed of elastin, albumin, globulin and mucin. Collagen constituting the dermal layer is mainly α1 (I) collagen and α1 (III) collagen (hereinafter referred to as "type III collagen"), type I collagen mainly maintains the skin structure, type III collagen It plays different functions mainly from the application of skin softness.

  The proportion of type I collagen and type III collagen in the skin changes with age. As type III collagen is also called fetal collagen, it is most abundant in the case of the fetus and more in the case of fetal skin with type III collagen, but begins to decrease from the 10s and continues to decrease, so type III collagen in the skin If you can increase the rate of aging, you can stop the aging of the skin with age and make the skin smooth and moist. In addition to skin, type III collagen is contained in connective tissues such as blood vessels, lungs and liver.

  The eggshell membrane-containing fine powder is digested and absorbed by about 46%, the hydrolyzed eggshell membrane component is digested and absorbed by almost 100%, and the eggshell membrane component taken up into the blood promotes expression of the type III collagen gene, In addition to promoting the production of type III collagen in the skin, it is possible to promote the production of type III collagen in blood vessels to increase the elasticity of the entire blood vessels, and also to suppress fibrosis in the liver. Both the eggshell membrane component-containing powder and the hydrolyzed eggshell membrane component used in the present invention have a function to promote the formation of type III collagen when digested and absorbed, but the hydrolyzed eggshell membrane component is almost 100% digested and absorbed. Is more effective.

  In addition, when the eggshell membrane component-containing powder and the hydrolyzed eggshell membrane component are used in combination, not only the formation promoting effect of type III collagen is further enhanced, but also combined with the improvement effect of intestinal flora, synergistically skin softening and antiaging effect Can be provided to improve the health condition and / or maintain well. It is preferable to mix 90 to 10 parts by weight of hydrolyzed eggshell membrane powder with 10 to 90 parts by weight of eggshell membrane-containing fine powder.

The jelly food of the present invention refers to a food in which a solution or suspension is made semisolid using a gelling agent such as agar or gelatin.
The content of the eggshell membrane-containing powder and / or the hydrolyzed eggshell membrane powder contained in the jelly food of the present invention is not particularly limited, but the content improves the intestinal flora improving effect and the type III collagen formation promoting effect. . For example, one jelly food can contain 10 to 240 mg, but since the eggshell membrane-containing component of the present invention is highly safe and has no fear of side effects, it has gel properties as long as other components are appropriately selected. It is contained in the range which does not cause trouble.

  Gelling agents used in the jelly food of the present invention include gelatin, polysaccharide thickeners gellan gum, carrageenan, xanthan gum, locust bean gum, guar gum, pectin, agar, gum arabic, glucomannan, cod gum, pullulan, tamarind It is at least one selected from seed polysaccharides, tragacanth gum, karaya gum, alginic acid, sodium alginate, macrohomopsis gum dextrin, and starches such as corn starch and tapioca starch. The amount of the gelling agent added to the jelly food is 0.01 to 5.0% (W / V), preferably 0.02 to 3.0% (W / V), and the hardness or the final jelly food is Depending on the texture, the type of gelling agent and the amount added can be determined.

The jelly food of the present invention may contain other raw materials other than the above-mentioned basic raw materials as long as the gel properties are not adversely affected. For example, saccharides, organic acids, vitamins, amino acids, minerals, proteins, collagen peptides, fruit juices, flavors, pigments and the like can be mentioned.
These other raw materials can be rapidly mixed and dissolved with the above-mentioned basic raw materials under heating by dissolving and dispersing them in a solvent such as water in advance.

  Organic acids serve not only to impart acidity to foods, but also to adjust sourness and harmonize tastes, but also have a role as preservation, oxidation prevention, and pH adjuster, and are usually added at about 0.1 to 1% by weight Do. The organic acid is selected from citric anhydride, citric acid, malic acid, tartaric acid, D-tartaric acid, ascorbic acid, succinic acid, maleic acid, malonic acid, L-glutamic acid hydrochloride, acetic acid, lactic acid and aspartic acid.

  In addition, from the viewpoint of suppressing acidity and facilitating eating, a trace amount of sweetener and flavor may be blended. As such sweeteners, saccharides such as glucose, fructose, sucrose, maltose, lactose, trehalose, erythritol, erythritol, xylitol, sorbitol, maltodextrin, acesulfame potassium, aspartame, stevia, sucralose, soumatin etc. These may be used alone or in combination of two or more. Also, the flavor is not particularly limited, and natural fruit juices such as grapefruit, apple, strawberry, pine, grape, etc., or synthetic flavor thereof can be used.

Vitamins are vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin F, vitamin H, vitamin P, vitamin P, pantothenic acid, choline, folic acid, inositol, niacin And at least one selected from para-aminobenzoic acid (PABA).
The amino acid is at least selected from alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine and the like It is one kind.

  The mineral is at least one selected from sodium chloride, potassium chloride, calcium chloride, magnesium chloride, calcium lactate, sodium lactate and the like. The above-mentioned protein is at least one selected from whey protein isolate (WPI) which is a milk-derived protein, whey protein concentrate (WPC), isolated soy protein (SPI), or a hydrolyzate thereof.

  In the production of a jelly food, a mixture is obtained by first mixing the above-mentioned components so as not to cause foaming using a stirring or mixing means such as a propeller mixer which does not involve air. Although there is no particular limitation on the order of addition of each component and the mixing method, the preparation of the mixture is carried out, for example, in a method including the following steps (A) and (B). (A) Dissolve the gelling agent in water and then heat to prepare an aqueous gelling agent solution, (B) While stirring the aqueous gelling agent solution, add eggshell membrane-containing powder and / or hydrolyzed eggshell membrane components, or add them The aqueous solution is added. Sugars, organic acids, vitamins, etc. are added between steps (A) and (B) or after step (B). The step of obtaining the mixture needs to be performed at a high temperature (for example, 60 ° C. or more, preferably 70 ° C. or more, more preferably 75 ° C. or more) above the gelling temperature.

The container for jelly food of the present invention is not particularly limited as to the container type as long as it is a sealable container, and the container is provided with a closed structure such as a screw type, pull top type, crimp type, etc. The food outlet may be sealed or the container itself may be openable. For example, a plastic container, a polyethylene terephthalate (PET) container, a bottle container, an aluminum container, a steel container, etc. can be illustrated.
In particular, it is desirable to seal only the required amount for one dose and individually package it in a smart pack package or a stick-shaped package container which does not bulk even when stored together.

  The stick-shaped packaging container is not particularly limited as long as it can be used as a packaging material for food, and examples thereof include packaging forms such as three-way sealed packaging and pillow packaging. The three-sided sealed packaging is a packaging form in which the folded edge is not sealed when the packaging material is folded in half and the three sides are sealed to form a bag. In addition, the pillow packaging is a form of packaging in which the contents are inserted into the cylindrical packaging material in the process of feeding the cylindrical packaging material in the vertical direction or in the horizontal direction, and the separated portion is sealed to form a bag. For the sealing, a form of heat sealing by heating and welding a packaging material can be used. Examples of the material of the packaging material include aluminum, nylon and plastics such as polyethylene and polypropylene. Further, in view of easiness of carrying and eating and the like, the size thereof is preferably about 5 to 12 cm in length, 1 to 5 cm in width, and about 10 to 50 g in internal capacity.

  Smart pack packaging is also referred to as a stand pack, and is a stand-type pouch that is stable and self-supporting. Holding a half-folded bottom sheet between the lower ends of the two body sheets, sealing the double-folded bottom sheet and the two side edges of the two body sheets, and further sealing the bottom sides in an arc. Thus, the bottom part has a bottom edge, which makes the pack body a stable stand. Such a stand pack type pack body is filled from the opening above it and seals the opening.

  Packaging of the jelly food of the present invention into a container is carried out by filling the container with the prepared jelly according to a known method generally performed by those skilled in the art. For example, as necessary, saccharides, organic acids, vitamins, amino acids, and the like as basic materials obtained by adding eggshell membrane-containing powder and / or hydrolyzed eggshell membrane component to a liquid to which a gelling agent has been added and then heating and dissolving it. Minerals, proteins, collagen peptides, fruit juice, flavors, pigments and the like may be added, mixed and dissolved under heat, filled in a molten state in a container, and solidified by cooling. In the case of smart pack packaging or stick-like packaging containers, after filling, the opening of the container is sealed by heat sealing and cooled and solidified in the container.

  Hereinafter, the present invention will be described in detail based on examples, but the present invention is not limited by these examples.

[Intestinal flora improvement function and enteritis improvement function of eggshell membrane-containing fine powder]
(1) Production of eggshell membrane-containing fine powder As an eggshell membrane-containing powder sample, one obtained by finely pulverizing product name “EM powder 300” (manufactured by Kewpie Corporation) using a jet mill was used. As a jet mill, a single track jet mill (manufactured by Seishin Co., Ltd., FS-4) is used, and the air volume is 1.2 m ³ / min, the power is 11 kw, and the maximum particle diameter is 800 mesh (about an opening) The grinding was carried out to about 20 μm). When the particle size after grinding is measured using a laser diffraction type particle size distribution analyzer (manufactured by Seishin Co., Ltd., LMS-30), the volume maximum particle size is 19.6 μm, and the volume average particle size is 5.8 μm (hereinafter referred to as It is called "800 mesh fine powder".

(2) Experimental animals 7-week-old male C57BL6 / J mice were purchased and acclimated for 3 days, and then divided into three groups (n = 8) so that the average body weights would be equal. For 7 days, either control AIN-93G basal regular diet (DSS group) or diet supplemented with 8% eggshell membrane 800 mesh fine powder (D-ESM group 8) was fed. Then, drinking water containing 1.5% (W / V) dextran sodium sulfate (DSS) (molecular weight 40 KDa; MP Biomedicals, Irvine, CA) was given for 9 days while both groups were fed the same experimental diet as before. It caused colitis. Normal control mice (CON group) were fed with AIN-93G basal regular diet and normal drinking water throughout the course of the experiment.
8% eggshell membrane 800 mesh fine powder fortified feed was adjusted with corn starch and casein to maintain calories and protein balance. The disease activity index (DAI) was monitored daily from the day of receiving DSS water, using three scores of mouse weight, stool firmness and blood stool.

(3) Bacterial metagenomic analysis of the cecum Metagenomic DNA was extracted from the contents of the mouse cecum using the QIAamp Stool Min kit (Qiagen, Hilden, Germany). In order to amplify variable regions 3 and 4 of the 16S rRNA gene, primers of (5′-CCTACGGGNGGCWGCAG-3 ′) and (5′-CAGGACTACHVGGGTATCTAATCC-3 ′) were used for amplification. Sequences were performed on DNA sequences having a length of 250 bp or more and an average quality score of 30 or more.
Representative sequences were classified according to the Greengenes OTU database using Ribosomal Database Project (RDP) classification.
Quantitative real-time PCR was performed to detect changes in segment bacteria and several pathogenic bacteria.

(4) Results of bacterial metagenome analysis According to the analysis at the bacterial gate level, the relative amounts of the three most widely distributed bacteria are significantly different among the three experimental groups (D-ESM8, DSS, CON) The As shown in FIG. 1, the Bacteriotides decreased from 67.0% in the CON group to 34.6% in the DSS group and recovered to 55.2% in the D-ESM 8 group. Firmicutes decreased from 26.5% in the CON group to 13.7% in the DSS group and recovered to 18.6% in the D-ESM 8 group. Proteobacteria increased from 0.9% in the CON group to 40.2% in the DSS group and recovered to 8.0% in the D-ESM 8 group.
Next, two classes of alpha diversity and beta diversity were evaluated. By alpha diversity analysis, mice in the DSS group had the lowest bacterial species abundance among the three groups (Figure 2).
This result was as expected, as the bacterial species in the gut is reduced, correlating with disease states including inflammatory bowel disease in humans and DSS-induced colitis in mice, but surprisingly, the eggshell membrane The intake restored the bacterial species abundance to the same extent as the CON group.

As shown in the upper row of FIG. 3, Enterobacteriaceae, which is a marker of enteritis and a marker of oxidative stress in human inflammatory bowel disease and mouse colitis, are more susceptible to the inflammatory DSS group (Proteobacteri all bacteria) compared to the CON group. 39% of the) were detected very frequently. In contrast, Enterobacteriaceae was detected very rarely in the D-ESM 8 group (about 6%). Similarly, DSS administration increased the number of Enterococcaceae but was lowered to near normal levels by eggshell membrane fine powder supplementation.
Metabolites of enteric bacteria such as short-chain fatty acids (SCFA) are the primary energy source of colonic cells and are thought to directly affect host gene expression, so the presence of SCFA-producing bacteria was assessed ( Bottom of FIG. 3). Although SCFA-producing bacteria such as Ruminococcaceae and Porphyromomonadaceae, which have been known to decrease in inflammatory bowel disease, were reduced in the DSS group, eggshell membrane intake improved them.

(5) Summary From the results of the above (4), intake of eggshell membrane component-containing fine powder not only increases the diversity of intestinal bacteria but also reduces the absolute number of pathogenic bacteria such as E. coli It has been shown to suppress the overgrowth of transformed filamentous fungi.
Thus, it was shown that intake of eggshell membrane-containing fine powder improves intestinal bacterial symbiotic balance disorder, enterotoxicosis and intestinal flora.

[Type III collagen formation promoting function of eggshell membrane-containing fine powder and hydrolyzed eggshell membrane powder]
Eggshell membrane 800 mesh fine powder and hydrolysed eggshell membrane powder were orally ingested to mice once a day for 17 days, and a supplement oral intake experiment was conducted. As a hydrolyzed eggshell membrane powder, a hydrolyzed eggshell membrane powder (Patent Document 3) having a pH of 3 to 6 was used.

  For 8-week-old hairless mice, hydrolyzed eggshell membrane powder was mixed with MediGel, and it was voluntarily consumed once daily for 17 days as a supplement in the active phase of the animal as a supplement. The amount equivalent to 1/2, 1 and 2 times the daily human intake is given (each n = 6), and the eggshell membrane 800 mesh fine powder is similarly used as the amount equivalent to 1-fold human daily intake It was ingested (n = 6). A hairless mouse without a supplement was used as a control. The type III collagen gene expression in skin and liver of each mouse after 17 days was examined by quantitative real time PCR.

Quantitative real-time PCR analysis was performed as follows.
Back skin and liver samples were taken from each mouse and ground in liquid nitrogen. After homogenization of whole skin tissue or whole liver tissue, total RNA was isolated and cDNA synthesis was performed using a trade name "Takara PrimeScript RT reagent kit". The real time PCR method was performed using a brand name "SYBR Premix Ex Taq II (Takara) on Thermal Cycler Dice Real Time System" (Takara). As a primer, a primer designed for amplification of a gene encoding Col3a1 (collagen type III) was used. As an internal standard, the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was similarly amplified. The PCR cycle was 30 cycles of initial denaturation at 95 ° C. for 30 cycles (denaturation at 95 ° C. for 5 seconds, annealing and elongation at 60 ° C. for 1 minute).

The expression level of type III collagen gene in skin is shown in FIG.
It has been shown that expression of the gene encoding type III collagen is increased in a dose-dependent manner (p = 0.08) in the back skin of mice orally ingested with hydrolyzed eggshell membrane powder. The expression of the gene encoding type III collagen was increased more effectively than the mice orally given eggshell membrane 800 mesh fine powder.

  With regard to the expression level of type III collagen gene in mice that ingested hydrolyzed eggshell membrane powder in the liver, there was no change from the control group in mice given 1-fold equivalent amount to be ingested by humans as a supplement, The expression of the type III collagen gene was significantly increased (p = 0.007) when a doubled amount was given. The increase in expression of type III collagen gene in the liver is expected to suppress liver fibrosis and improve the environment of blood vessels.

[Production of jelly food containing eggshell membrane-containing powder]
18 parts by weight of a commercially available gelling agent containing carrageenan, locust bean gum, xanthan gum and glucomannan in a weight ratio of 16: 25: 25: 10 is added to 100 parts by weight of water and stirred for 10 minutes to prevent foaming. The mixture was heated to ° C. and stirred for additional 30 minutes to prepare an aqueous solution of a gelling agent. While stirring the gelling agent aqueous solution, 2.7 parts by weight of eggshell membrane-containing powder was added. The pH of this aqueous solution of gelling agent was adjusted to pH 3.5 to 4.2 with citric acid, and flavoring, sweetening and antifoaming agents were added and stirred. The mixture thus obtained is heated and melted at 90 ° C. for 5 minutes, and filled at about 85 ° C. into a smart pack packaging container of 135 mm long × 65 mm wide having a pinched portion of 30 mm long at the upper opening. The top opening was sealed to produce a smart pack packaged jelly food having a content of 40 g.

The eggshell membrane-containing fine powder was found to have its remaining components to prevent the growth of bad bacteria out of enteric bacteria and to improve the enteric flora. In addition, about 46% of the eggshell membrane-containing fine powder is digested and absorbed, about 100% of the hydrolyzed eggshell membrane component is digested and absorbed, and the expression of type III collagen gene is promoted by the eggshell membrane component taken into blood. This not only promotes the formation of type III collagen in the skin, but also can promote the generation of type III collagen in the blood vessel and increase the elasticity of the entire blood vessel.
By adding these active ingredients to jelly food which can eat easily and in a necessary amount, synergy effect of intestinal flora improving function and type III collagen generation promoting function enhances skin effect and anti-aging effect, and health It is possible to provide a superior functional jelly food which can improve the condition and / or maintain well.

Claims (8)

  A jelly food for promoting type III collagen formation, which comprises an eggshell membrane-containing powder and / or a hydrolyzed eggshell membrane component as an active ingredient.   The gel food for promoting type III collagen formation according to claim 1, wherein an eggshell membrane-containing powder is added as an active ingredient to improve intestinal flora.   The eggshell membrane-containing fine powder according to claim 1 or 2, wherein the eggshell membrane-containing powder is an eggshell membrane-containing fine powder having a volume average particle size of 6 μm or less, or a volume maximum particle size of 20 μm or less.   The jelly food according to claim 1, wherein the hydrolyzed eggshell membrane component is a hydrolyzed eggshell membrane powder.   The gel for promoting formation of type III collagen according to claim 1, wherein 10 to 90 parts by weight of the eggshell membrane-containing powder and 90 to 10 parts by weight of the hydrolyzed eggshell membrane component are contained as active ingredients. Food.   The jelly food for promoting type III collagen formation according to any one of claims 1 to 5, wherein the promotion of type III collagen formation promotes the expression of a type III collagen gene in cells.   The method according to claim 2 or 5, wherein the improvement of intestinal flora is due to the fact that in the intestine, components which are not absorbed and remain in the intestine increase the diversity of intestinal bacteria and decrease the number of pathogenic bacteria. Jelly food for promoting type III collagen formation.   The jelly food according to any one of claims 1 to 7, which is individually packaged in a smart pack package or a stick package container.