JP2018531013A5 - - Google Patents
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- Publication number
- JP2018531013A5 JP2018531013A5 JP2018518522A JP2018518522A JP2018531013A5 JP 2018531013 A5 JP2018531013 A5 JP 2018531013A5 JP 2018518522 A JP2018518522 A JP 2018518522A JP 2018518522 A JP2018518522 A JP 2018518522A JP 2018531013 A5 JP2018531013 A5 JP 2018531013A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- engineered nucleic
- acid construct
- acid encoding
- engineered
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000039446 nucleic acids Human genes 0.000 claims 29
- 108020004707 nucleic acids Proteins 0.000 claims 29
- 150000007523 nucleic acids Chemical class 0.000 claims 29
- 101710163270 Nuclease Proteins 0.000 claims 9
- 230000004568 DNA-binding Effects 0.000 claims 7
- 108091033409 CRISPR Proteins 0.000 claims 4
- 102000015694 estrogen receptors Human genes 0.000 claims 4
- 108010038795 estrogen receptors Proteins 0.000 claims 4
- 238000011144 upstream manufacturing Methods 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108020005004 Guide RNA Proteins 0.000 claims 3
- 230000001939 inductive effect Effects 0.000 claims 3
- 238000011830 transgenic mouse model Methods 0.000 claims 3
- 241000699666 Mus <mouse, genus> Species 0.000 claims 2
- 241000699660 Mus musculus Species 0.000 claims 2
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 2
- NKANXQFJJICGDU-QPLCGJKRSA-N Tamoxifen Chemical compound C=1C=CC=CC=1C(/CC)=C(C=1C=CC(OCCN(C)C)=CC=1)/C1=CC=CC=C1 NKANXQFJJICGDU-QPLCGJKRSA-N 0.000 claims 2
- 102000034356 gene-regulatory proteins Human genes 0.000 claims 2
- 108091006104 gene-regulatory proteins Proteins 0.000 claims 2
- 239000012212 insulator Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 101001000998 Homo sapiens Protein phosphatase 1 regulatory subunit 12C Proteins 0.000 claims 1
- 102100035620 Protein phosphatase 1 regulatory subunit 12C Human genes 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000004043 responsiveness Effects 0.000 claims 1
- 229960001603 tamoxifen Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562242884P | 2015-10-16 | 2015-10-16 | |
| US62/242,884 | 2015-10-16 | ||
| PCT/IB2016/001902 WO2017064566A2 (en) | 2015-10-16 | 2016-10-14 | Inducible modification of a cell genome |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2018531013A JP2018531013A (ja) | 2018-10-25 |
| JP2018531013A6 JP2018531013A6 (ja) | 2018-12-13 |
| JP2018531013A5 true JP2018531013A5 (OSRAM) | 2019-11-28 |
| JP6936952B2 JP6936952B2 (ja) | 2021-09-22 |
Family
ID=58054378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018518522A Active JP6936952B2 (ja) | 2015-10-16 | 2016-10-14 | 細胞ゲノムの誘導性改変 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10947559B2 (OSRAM) |
| EP (1) | EP3362561A2 (OSRAM) |
| JP (1) | JP6936952B2 (OSRAM) |
| CN (1) | CN108431225A (OSRAM) |
| AU (1) | AU2016337408B2 (OSRAM) |
| CA (1) | CA3001518A1 (OSRAM) |
| WO (1) | WO2017064566A2 (OSRAM) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| EP3365356B1 (en) | 2015-10-23 | 2023-06-28 | President and Fellows of Harvard College | Nucleobase editors and uses thereof |
| WO2017078631A1 (en) | 2015-11-05 | 2017-05-11 | Agency For Science, Technology And Research | Chemical-inducible genome engineering technology |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| EP3526320A1 (en) | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| JP7780858B2 (ja) * | 2017-06-16 | 2025-12-05 | ロンザ リミテッド | 生物製剤の製造のためのユニバーサル自己調節性哺乳動物細胞株プラットフォーム |
| KR101928659B1 (ko) * | 2017-06-19 | 2018-12-13 | 서울대학교병원 | Dna 변형 유도 단백질 복합체 및 이를 암호화하는 유전자를 포함하는 재조합 벡터 |
| WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| WO2020028533A1 (en) * | 2018-08-01 | 2020-02-06 | Yale University | Compositions and methods for identification of membrane targets for enhancement of t cell activity against cancer |
| KR20210075126A (ko) * | 2018-10-11 | 2021-06-22 | 아이드제노시쉐 테크니쉐 훽슐레 쥐리히 | 고도로 콤팩트한 다중-입력 논리 게이트를 코딩하는 핵산 벡터를 사용하여 질환을 치료하는 방법 |
| US11851663B2 (en) * | 2018-10-14 | 2023-12-26 | Snipr Biome Aps | Single-vector type I vectors |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| MX2021011426A (es) | 2019-03-19 | 2022-03-11 | Broad Inst Inc | Metodos y composiciones para editar secuencias de nucleótidos. |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021111280A1 (en) * | 2019-12-04 | 2021-06-10 | Crispr Therapeutics Ag | Regulatable expression systems |
| MX2022014008A (es) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo. |
| CN111705080A (zh) * | 2020-05-14 | 2020-09-25 | 广州市第八人民医院 | Hbv非人动物模型的构建方法及其应用 |
| CN116262927B (zh) * | 2021-12-13 | 2024-04-26 | 中国科学院微生物研究所 | 基于CRISPR/Cas系统调控基因表达的方法及其应用 |
| WO2024231559A1 (en) | 2023-05-11 | 2024-11-14 | Astrazeneca Ab | Methods of treatment of chronic kidney disease |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| WO2005123916A2 (en) | 2004-06-21 | 2005-12-29 | Novo Nordisk Health Care Ag | Glycosylation-disrupted factor vii variants |
| JP4811765B2 (ja) * | 2004-06-22 | 2011-11-09 | 学校法人東海大学 | 外来遺伝子の誘導発現の制御が可能な発現ベクター |
| KR20230065381A (ko) | 2012-07-25 | 2023-05-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 유도 ｄｎａ 결합 단백질 및 게놈 교란 도구 및 이의 적용 |
| CA2894668A1 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas systems and methods for altering expression of gene products in eukaryotic cells |
-
2016
- 2016-10-14 EP EP16837979.0A patent/EP3362561A2/en active Pending
- 2016-10-14 AU AU2016337408A patent/AU2016337408B2/en active Active
- 2016-10-14 WO PCT/IB2016/001902 patent/WO2017064566A2/en not_active Ceased
- 2016-10-14 CN CN201680074288.4A patent/CN108431225A/zh active Pending
- 2016-10-14 JP JP2018518522A patent/JP6936952B2/ja active Active
- 2016-10-14 CA CA3001518A patent/CA3001518A1/en active Pending
- 2016-10-14 US US15/768,482 patent/US10947559B2/en active Active
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