JP2018529106A - タイヤ用取扱装置 - Google Patents
タイヤ用取扱装置 Download PDFInfo
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- JP2018529106A JP2018529106A JP2018524528A JP2018524528A JP2018529106A JP 2018529106 A JP2018529106 A JP 2018529106A JP 2018524528 A JP2018524528 A JP 2018524528A JP 2018524528 A JP2018524528 A JP 2018524528A JP 2018529106 A JP2018529106 A JP 2018529106A
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- 238000005259 measurement Methods 0.000 claims abstract description 50
- 238000000034 method Methods 0.000 claims abstract description 38
- 238000002073 fluorescence micrograph Methods 0.000 claims abstract description 16
- 239000007850 fluorescent dye Substances 0.000 claims description 13
- 230000005284 excitation Effects 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 6
- 238000001514 detection method Methods 0.000 description 23
- 241001270131 Agaricus moelleri Species 0.000 description 7
- 230000002596 correlated effect Effects 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000004397 blinking Effects 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 238000004061 bleaching Methods 0.000 description 3
- 230000012447 hatching Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012935 Averaging Methods 0.000 description 2
- 238000005314 correlation function Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000026447 protein localization Effects 0.000 description 1
- 238000003530 single readout Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T3/00—Geometric image transformation in the plane of the image
- G06T3/40—Scaling the whole image or part thereof
- G06T3/4053—Super resolution, i.e. output image resolution higher than sensor resolution
Abstract
Description
Kτ=((I(tn)−I平均)x(I(tm)−I平均))平均
を用いて算出される。その際、I(tn)は前の測定時点tnでの振幅、I(tm)は後の測定時点tmでの振幅、m=n+τであり、I平均は全ての振幅の平均値として算出される平均振幅である。
Kτ=((I(tn)−I平均)x(I(tm)−I平均))平均
に従ってもたらされ、その際、先の振幅I(tn)及び後の振幅I(tm)の測定時点は互いに時間シフトτを有している。
Kτ=((I(tn)−I平均)x(I(tm)−I平均))平均/I平均 2
に従って、もたらされる。
又は、振幅の最大値の2乗を用いて、以下の数式
Kτ=((I(tn)−I平均)x(I(tm)−I平均))平均/I最大 2
に従って、もたらされる。
Claims (8)
- デジタル蛍光画像を作成するための方法であって、蛍光性の色素を有する試料が、レーザー走査顕微鏡の対象面に配され、また時間的に連続して、1ピクセル単位でピクセル滞在時間(T)に渡って励起光で作用され、それによりピクセルごとに放射光が発生され、当該放射光は検出器によって検出され、それぞれ1つの測定時点に割り当てられた振幅(I)の連続へと変換され、そこから全振幅が形成され、全てのピクセルの複数の全振幅が、1枚の蛍光画像へとまとめられる方法において、
後の測定時点(tm)から始まる複数の振幅(I)の連続が、前の測定時点(tn)から始まる複数の振幅の同様の連続と、少なくとも一度自己相関され、前記前の測定時点(tn)は前記後の測定時点(tm)に対して時間シフト(τ)を有しており、時間シフトの1つに特有の1つの相関振幅(Kτ)がその都度形成され、また、形成された相関振幅(Kτ)の少なくとも1つから前記全振幅が算出されること、
を特徴とする方法。 - 請求項1に記載方法において、
前記相関振幅(Kτ)が以下の式
Kτ=((I(tn)−I平均)x(I(tm)−I平均))平均
を用いて算出され、
その際、I(tn)は前記前の測定時点(tn)の振幅(I)、I(tm)は前記後の測定時点(tm)の振幅(I)、m=n+τ、そしてI平均は振幅(I)の平均値であること、
を特徴とする方法。 - 請求項2に記載方法において、
前記相関振幅(Kτ)が、前記振幅(I)の平均値の2乗で割られることにより正規化されること、
を特徴とする方法。 - 請求項1から3の何れか一項に記載方法において、
異なる時間シフト(τ)に対して前記相関振幅(Kτ)が算出され、また、比較の上最大の相関振幅(Kτ)を有する当該時間シフト(τ)が確定され、また全振幅として用いられること、
を特徴とする方法。 - 請求項4に記載方法において、
前記比較の上最大の全振幅を有する前記時間シフト(τ)が、蛍光性物質に割り当てられ、また、データベースに保存されること、
を特徴とする方法。 - 請求項1から5の何れか一項に記載方法において、
異なる時間シフト(τ)に対して前記相関振幅(Kτ)が算出され、また、複数の前記相関振幅(Kτ)の平均値が確定され、また全振幅として用いられること、
を特徴とする方法。 - 請求項1から6の何れか一項に記載方法において、
異なる時間シフト(τ)に対して前記相関振幅(Kτ)が算出され、また、複数の前記相関振幅(Kτ)の合計が確定され、全振幅として用いられること、
を特徴とする方法。 - 請求項1から7の何れか一項に記載方法において、
前記測定時点の総数が400を超えており、また、前記時間シフト(τ)が50から150の間にあること、
を特徴とする方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102015112628.1A DE102015112628A1 (de) | 2015-07-31 | 2015-07-31 | Verfahren zur Erzeugung eines digitalen Fluoreszenzbildes |
DE102015112628.1 | 2015-07-31 | ||
PCT/DE2016/100341 WO2017020887A1 (de) | 2015-07-31 | 2016-07-27 | Verfahren zur erzeugung eines digitalen fluoreszenzbildes |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2018529106A true JP2018529106A (ja) | 2018-10-04 |
JP2018529106A5 JP2018529106A5 (ja) | 2019-09-05 |
JP6820332B2 JP6820332B2 (ja) | 2021-01-27 |
Family
ID=56740726
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2018524528A Active JP6820332B2 (ja) | 2015-07-31 | 2016-07-27 | デジタル蛍光画像を作成するための方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US10539505B2 (ja) |
EP (1) | EP3329256B1 (ja) |
JP (1) | JP6820332B2 (ja) |
CN (1) | CN107850543B (ja) |
DE (1) | DE102015112628A1 (ja) |
WO (1) | WO2017020887A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024034344A1 (ja) * | 2022-08-09 | 2024-02-15 | キヤノン株式会社 | 分析方法 |
Citations (8)
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EP0409318A2 (en) * | 1989-07-19 | 1991-01-23 | ENIRICERCHE S.p.A. | Method of determining thread speed |
JP2002071567A (ja) * | 2000-07-21 | 2002-03-08 | Iss (Usa) Inc | 高速高スループット分光計および方法 |
JP2005017282A (ja) * | 2003-05-30 | 2005-01-20 | Olympus Corp | 受光ユニットおよびそれを含む測定装置 |
JP2008527359A (ja) * | 2005-01-17 | 2008-07-24 | バイオフォス・アーゲー | 粒子の動的パラメータを測定するための方法およびデバイス |
US20090290780A1 (en) * | 2006-07-17 | 2009-11-26 | Karsten Kottig | Analysis method for chemical and/or biological samples |
JP2012529119A (ja) * | 2009-06-02 | 2012-11-15 | ソーファスト ゲーエムベーハー | 超解像光ゆらぎイメージング(sofi) |
JP2013190221A (ja) * | 2012-03-12 | 2013-09-26 | Mitsui Eng & Shipbuild Co Ltd | 蛍光検出装置及び蛍光検出方法 |
JP2013213756A (ja) * | 2012-04-03 | 2013-10-17 | Institute Of Physical & Chemical Research | 多重蛍光画像の画像解析のための装置、システム、方法、およびプログラム |
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2015
- 2015-07-31 DE DE102015112628.1A patent/DE102015112628A1/de not_active Withdrawn
-
2016
- 2016-07-27 JP JP2018524528A patent/JP6820332B2/ja active Active
- 2016-07-27 EP EP16754174.7A patent/EP3329256B1/de active Active
- 2016-07-27 WO PCT/DE2016/100341 patent/WO2017020887A1/de active Application Filing
- 2016-07-27 CN CN201680044740.2A patent/CN107850543B/zh active Active
- 2016-07-27 US US15/746,732 patent/US10539505B2/en active Active
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EP0409318A2 (en) * | 1989-07-19 | 1991-01-23 | ENIRICERCHE S.p.A. | Method of determining thread speed |
JP2002071567A (ja) * | 2000-07-21 | 2002-03-08 | Iss (Usa) Inc | 高速高スループット分光計および方法 |
JP2005017282A (ja) * | 2003-05-30 | 2005-01-20 | Olympus Corp | 受光ユニットおよびそれを含む測定装置 |
JP2008527359A (ja) * | 2005-01-17 | 2008-07-24 | バイオフォス・アーゲー | 粒子の動的パラメータを測定するための方法およびデバイス |
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JP2013190221A (ja) * | 2012-03-12 | 2013-09-26 | Mitsui Eng & Shipbuild Co Ltd | 蛍光検出装置及び蛍光検出方法 |
JP2013213756A (ja) * | 2012-04-03 | 2013-10-17 | Institute Of Physical & Chemical Research | 多重蛍光画像の画像解析のための装置、システム、方法、およびプログラム |
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WO2024034344A1 (ja) * | 2022-08-09 | 2024-02-15 | キヤノン株式会社 | 分析方法 |
Also Published As
Publication number | Publication date |
---|---|
EP3329256B1 (de) | 2022-09-21 |
EP3329256A1 (de) | 2018-06-06 |
WO2017020887A1 (de) | 2017-02-09 |
CN107850543B (zh) | 2021-01-15 |
JP6820332B2 (ja) | 2021-01-27 |
US10539505B2 (en) | 2020-01-21 |
CN107850543A (zh) | 2018-03-27 |
US20190003967A1 (en) | 2019-01-03 |
DE102015112628A1 (de) | 2017-02-02 |
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