JP2018500341A5 - - Google Patents
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- JP2018500341A5 JP2018500341A5 JP2017533632A JP2017533632A JP2018500341A5 JP 2018500341 A5 JP2018500341 A5 JP 2018500341A5 JP 2017533632 A JP2017533632 A JP 2017533632A JP 2017533632 A JP2017533632 A JP 2017533632A JP 2018500341 A5 JP2018500341 A5 JP 2018500341A5
- Authority
- JP
- Japan
- Prior art keywords
- monomer
- refold buffer
- unfolded
- concentration
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 102000003814 Interleukin-10 Human genes 0.000 claims description 16
- 108090000174 Interleukin-10 Proteins 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 9
- 239000000178 monomer Substances 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000588724 Escherichia coli Species 0.000 claims description 3
- 108010024636 Glutathione Proteins 0.000 claims description 2
- 108010053070 Glutathione Disulfide Proteins 0.000 claims description 2
- 101001033233 Homo sapiens Interleukin-10 Proteins 0.000 claims description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 2
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 claims description 2
- 102000052620 human IL10 Human genes 0.000 claims description 2
- 210000003000 inclusion body Anatomy 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 15
- 229940076144 interleukin-10 Drugs 0.000 claims 9
- 150000001413 amino acids Chemical class 0.000 claims 1
- 238000004140 cleaning Methods 0.000 claims 1
- 238000000502 dialysis Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000000108 ultra-filtration Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462096359P | 2014-12-23 | 2014-12-23 | |
| US62/096,359 | 2014-12-23 | ||
| PCT/US2015/067135 WO2016106229A1 (en) | 2014-12-23 | 2015-12-21 | Methods of improving yield in recombinant protein production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018500341A JP2018500341A (ja) | 2018-01-11 |
| JP2018500341A5 true JP2018500341A5 (enExample) | 2019-02-07 |
Family
ID=56151492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017533632A Withdrawn JP2018500341A (ja) | 2014-12-23 | 2015-12-21 | 組み換えタンパク質の生産における収量改善法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20170362291A1 (enExample) |
| EP (1) | EP3237441A4 (enExample) |
| JP (1) | JP2018500341A (enExample) |
| CN (1) | CN107108712A (enExample) |
| AU (1) | AU2015369808A1 (enExample) |
| CA (1) | CA2969574A1 (enExample) |
| HK (1) | HK1245297A1 (enExample) |
| WO (1) | WO2016106229A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107106655A (zh) | 2014-10-22 | 2017-08-29 | 阿尔莫生物科技股份有限公司 | 使用白细胞介素‑10治疗疾病和病症的方法 |
| WO2016126615A1 (en) | 2015-02-03 | 2016-08-11 | Armo Biosciences, Inc. | Methods of using interleukin-10 for treating diseases and disorders |
| CN107847583A (zh) | 2015-05-28 | 2018-03-27 | 阿尔莫生物科技股份有限公司 | 用于治疗癌症的聚乙二醇化白细胞介素‑10 |
| EP3341012B1 (en) | 2015-08-25 | 2025-07-30 | Armo Biosciences, Inc. | Methods of using interleukin-10 for treating diseases and disorders |
| KR20210110294A (ko) * | 2018-11-07 | 2021-09-07 | 어플라이드 몰레큘라 트랜스포트 인크. | 통과세포외배출용 전달 구조체 및 관련 방법 |
| US12239793B2 (en) | 2019-07-09 | 2025-03-04 | O2Matic Aps | Device for regulating oxygen for automated oxygen therapy |
| WO2021034727A1 (en) | 2019-08-16 | 2021-02-25 | Applied Molecular Transport Inc. | Compositions, formulations, and interleukin production and purification |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998020033A2 (en) * | 1996-11-06 | 1998-05-14 | Schering Corporation | RENATURATION AND PURIFICATION OF VIRAL INTERLEUKIN-10 (vIL-10) |
| CN1306030C (zh) * | 2001-09-28 | 2007-03-21 | 中山大学 | 一种人白细胞介素－10基因序列及含该基因序列的大肠杆菌 |
| JPWO2005033307A1 (ja) * | 2003-09-30 | 2006-12-14 | 技術研究組合 生物分子工学研究所 | 新規のリフォールディング方法およびその方法によって得られたタンパク質 |
| EP1931704B1 (en) * | 2005-10-04 | 2010-12-15 | ZymoGenetics, L.L.C. | Production and purification of il-29 |
| WO2008008975A2 (en) * | 2006-07-14 | 2008-01-17 | Genentech, Inc. | Refolding of recombinant proteins |
| US8759617B2 (en) * | 2010-09-21 | 2014-06-24 | National Institute Of Agrobiological Sciences | Method for extraction and purification of recombinant proteins from transgenic plants |
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2015
- 2015-12-21 CA CA2969574A patent/CA2969574A1/en not_active Abandoned
- 2015-12-21 WO PCT/US2015/067135 patent/WO2016106229A1/en not_active Ceased
- 2015-12-21 AU AU2015369808A patent/AU2015369808A1/en not_active Abandoned
- 2015-12-21 US US15/532,254 patent/US20170362291A1/en not_active Abandoned
- 2015-12-21 HK HK18104785.1A patent/HK1245297A1/zh unknown
- 2015-12-21 CN CN201580073185.1A patent/CN107108712A/zh active Pending
- 2015-12-21 EP EP15874258.5A patent/EP3237441A4/en not_active Withdrawn
- 2015-12-21 JP JP2017533632A patent/JP2018500341A/ja not_active Withdrawn