JP2018070640A - 幹細胞増強治療法 - Google Patents
幹細胞増強治療法 Download PDFInfo
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Abstract
【解決手段】ヒトの未分化幹細胞で発現されたMUC1タンパク質のエピトープに特異的に結合する抗体であって、該抗体は、特定のペプチド配列の少なくとも6つの連続したアミノ酸に特異的に結合し、かつ該抗体は、他の特定のペプチド配列に、結合しないか、実質的に殆ど結合しないものである、抗体。患者の幹細胞への刺激によって利益を得る患者を処置する方法であって、患者に、ヒトの未分化の幹細胞で発現されりMUC1タンパク質のエピトープに特異的に結合する抗体を投与することを含む治療。
【選択図】図1
Description
(i) その配列が、SEQ ID NOS:1-11の配列をもつペプチドのうちのいずれかであるペプチドを認識する抗体の混合セットの生成;
(ii) SEQ ID NO:4、SEQ ID NO:5、あるいはSEQ ID NO:6のペプチドに、しかしSEQ ID NO:3;のペプチドにではない、結合する抗体を選択すること;
(iii)表面上に吸着された時、幹細胞の付着を促進する抗体を選択すること;
(iv)また、癌細胞に影響なしで、二価の形式で、幹細胞の成長を刺激し、および一価の形式で、肝細胞の成長を阻害する抗体を選択すること。
あるいは、抗体は、SEQ ID NO:5のペプチド、SEQ ID NO:6のペプチド、又は結合したSEQ ID NOS:4と5からの連続するアミノ酸を含んでいるペプチドに結合する。さらにより好適な実施態様では、幹細胞抗体のセットに属する抗体は、MUC1陽性の癌細胞にではなくヒトの幹および/または先祖細胞に結合する。さらに、幹細胞抗体セットに属する抗体は、それらが二価の場合に幹および/または先祖細胞増殖を刺激し、かつ一価の場合(例えば、Fab)に、幹および/または先祖細胞増殖を阻害しうるそれらの能力によって選択される。まだもっと好適な実施態様では、二価型の抗体も一価型の抗体も癌細胞の成長に影響しない。
(SEQ ID NO: 12) MIN-C2重鎖可変領域。
(SEQ ID NO: 13) MIN-C2重鎖可変領域。
(SEQ ID NO: 14) MIN-C2カッパ鎖可変領域。
(SEQ ID NO: 15) MIN-C2カッパ鎖可変領域。
(SEQ ID NO: 16) MIN-E6重鎖-7可変領域。
(SEQ ID NO: 17) MIN-E6重鎖-7可変領域。
(SEQ ID NO: 18) MIN-E6重鎖-8可変領域。
(SEQ ID NO: 19) MIN-E6重鎖-8可変領域。
(SEQ ID NO: 20) MIN-E6カッパ鎖可変領域。
(SEQ ID NO: 21) MIN-E6カッパ鎖可変領域。
(SEQ ID NO: 22) MIN-C2Fab重鎖。
(SEQ IDNO: 23) MIN-C2Fabカッパ鎖。
(SEQ ID NO: 24) MIN-C2Fab重鎖。
(SEQ IDNO: 25) MIN-C2Fabカッパ鎖。
(SEQ IDNO: 26) MIN-C2軽CL領域アミノ酸配列。
(SEQ IDNO: 27) MIN-C2重鎖CH1領域アミノ酸配列。
(SEQ ID NO: 28) MIN-C2軽鎖可変フレームワーク領域1(FWR1)アミノ酸配列。
(SEQ ID NO: 29) MIN-E6軽鎖可変フレームワーク領域1(FWR1)アミノ酸配列。
(SEQ ID NO: 30) MIN-C2軽鎖可変相補性決定領域1(CDR1)アミノ酸配列。
(SEQ ID NO: 31) MIN-E6軽鎖可変相補性決定領域1(CDR1)。
(SEQ ID NO: 32) MIN-C2軽鎖可変フレームワーク領域2(FWR2)アミノ酸配列。
(SEQ ID NO: 33) MIN-E6軽鎖可変フレームワーク領域2(FWR2)アミノ酸配列。
(SEQ ID NO: 34) MIN-C2軽鎖可変相補性決定領域2(CDR2)アミノ酸配列。
(SEQ ID NO: 35) MIN-E6軽鎖可変相補性決定領域2(CDR2)アミノ酸配列。
(SEQ ID NO: 36) MIN-C2軽鎖可変フレームワーク領域3(FWR3)アミノ酸配列。
(SEQ ID NO: 37) MIN-E6軽鎖可変フレームワーク領域3(FWR3)アミノ酸配列。
(SEQ ID NO: 38) MIN-C2軽鎖可変相補性決定領域3(CDR3)アミノ酸配列。
(SEQ ID NO: 39) MIN-E6軽鎖可変相補性決定領域3(CDR3)アミノ酸配列。
(SEQ ID NO: 40) MIN-C2重鎖可変フレームワーク領域1(FWR1)アミノ酸配列。
(SEQ ID NO: 41) MIN-E6-7重鎖可変フレームワーク領域1(FWR1)アミノ酸配列。
(SEQ ID NO: 42) MIN-E6-8重鎖可変フレームワーク領域1(FWR1)アミノ酸配列。
(SEQ ID NO: 43) MIN-C2重鎖可変相補性決定領域1(CDR1)アミノ酸配列。
(SEQ ID NO: 44) MIN-E6-7重鎖可変相補性決定領域1(CDR1)アミノ酸配列。
(SEQ ID NO: 45) MIN-E6-8重鎖可変相補性決定領域1(CDR1)アミノ酸配列。
(SEQ ID NO: 46) MIN-C2重鎖可変フレームワーク領域2(FWR2)アミノ酸配列。
(SEQ ID NO: 47) MIN-E6-7重鎖可変フレームワーク領域2(FWR2)アミノ酸配列。
(SEQ ID NO: 48) MIN-E6-8重鎖可変フレームワーク領域2(FWR2)アミノ酸配列。
(SEQ ID NO: 49) MIN-C2重鎖可変相補性決定領域2(CDR2)アミノ酸配列。
(SEQ ID NO: 50) MIN-E6-7重鎖可変相補性決定領域2(CDR2)アミノ酸配列。
(SEQ ID NO: 51) MIN-E6-8重鎖可変相補性決定領域2(CDR2)アミノ酸配列。
(SEQ ID NO: 52) MIN-C2重鎖可変フレームワーク領域3(FWR3)アミノ酸配列。
(SEQ ID NO: 53) MIN-E6-7重鎖可変フレームワーク領域3(FWR3)アミノ酸配列。
(SEQ ID NO: 54) MIN-E6-8重鎖可変フレームワーク領域3(FWR3)アミノ酸配列。
(SEQ ID NO: 55) MIN-C2重鎖可変相補性決定領域3(CDR3)アミノ酸配列。
(SEQ ID NO: 56) MIN-E6-7重鎖可変相補性決定領域3(CDR3)アミノ酸配列。
(SEQ ID NO: 57) MIN-E6-8重鎖可変相補性決定領域3(CDR3)アミノ酸配列。
(SEQ ID NO: 58) IGHV3(Igblastからの名前)。:
FWR1: MIN-C2の可変重鎖領域への84.7%相同性(249/294)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 59) IGHV3(Igblastからの名前)。:
FWR2: MIN-C2の可変重鎖領域への84.7%相同性(249/294)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 60) IGHV3(Igblastからの名前)。:
FWR3: MIN-C2の可変重鎖領域への84.7%相同性(249/294)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 61) IGkV7(Igblastからの名前)。:
FWR1: MIN-C2の可変軽鎖領域への76.4%相同性(226/296)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 62) IGkV7(Igblastからの名前)。:
FWR2: MIN-C2の可変軽鎖領域への76.4%相同性(226/296)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 63) IGkV7(Igblastからの名前)。:
FWR 3: MIN-C2の可変軽鎖領域への76.4%相同性(226/296)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 64) IGHV3(Igblastからの名前)。:
FWR1: MIN-E6の可変重鎖領域への84.1%相同性(249/296)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 65) IGHV3(Igblastからの名前)。:
FWR2: MIN-E6の可変重鎖領域への84.1%相同性(249/296)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 66) IGHV3(Igblastからの名前)。:
FWR3: MIN-E6の可変重鎖領域への84.1%相同性(249/296)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 67) IGkV3(Igblastからの名前)。:
FWR1: MIN-E6の可変軽鎖領域への69.5%相同性(187/269)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 68) IGkV3(Igblastからの名前)。:
FWR2: MIN-E6の可変軽鎖領域への69.5%相同性(187/269)を備えたヒトのIgG抗体フレームワーク領域配列。
(SEQ ID NO: 69) IGkV3(Igblastからの名前)。:
FWR3: MIN-E6の可変軽鎖領域への69.5%相同性(187/269)を備えたヒトのIgG抗体フレームワーク領域配列。
Claims (2)
- ヒトの未分化幹細胞で発現されたMUC1タンパク質のエピトープに特異的に結合する抗体であって、該抗体は、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:9、SEQ ID NO:10あるいはSEQ ID NO:11のペプチドの少なくとも6つの連続したアミノ酸に特異的に結合し、かつ
該抗体は、SEQ ID NO:7あるいはSEQ ID NO:8のペプチドに、結合しないか、実質的に殆ど結合しないものである、抗体。 - MUC1タンパク質のエピトープに特異的に結合する抗体であって、SEQ ID NO:8のペプチドに特異的に結合するが、SEQ ID NO:11のペプチドに結合しないか、実質的に殆ど結合しない抗体。
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PCT/US2012/060684 WO2013059373A2 (en) | 2011-10-17 | 2012-10-17 | Media for stem cell proliferation and induction |
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PCT/US2013/025981 WO2013123084A1 (en) | 2012-02-13 | 2013-02-13 | Method for detecting circulating fetal cells |
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KR20210107166A (ko) * | 2014-04-07 | 2021-08-31 | 미네르바 바이오테크놀로지 코포레이션 | 항-nme 항체 |
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CN116333140A (zh) | 2016-04-08 | 2023-06-27 | 埃缇健康公司D/B/A泽尔拜尔 | 网蛋白-1结合抗体及其用途 |
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