JP2018070548A - Placenta extract-containing composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition which synergistically improves production of various growth factors in dermal fibroblasts by using a placenta extract and a specific plant in combination.SOLUTION: There is provided a composition which comprises (A) a placenta extract and (B) one or two or more selected from Crocus, Melilotus officinalis, Valeriana fauriei and Rubus suavissimus, In particular, there is provided a composition using a placenta extract, Crocus, Melilotus officinalis, Valeriana fauriei and Rubus suavissimus in combination. The composition is useful as a medicine, a food, a quasi-drug and a cosmetic, particularly useful as a quasi-drug and a cosmetic.SELECTED DRAWING: Figure 10

Description

  The present invention relates to a composition comprising a placenta extract and a specific plant in combination.

    The placenta extract is an extract obtained by extracting the placenta of animals and plants, and is actively used in the fields of beauty, health or medicine. Non-Patent Document 1 describes the effectiveness of placenta extract as an external preparation as follows. In the dermis, the proliferation of dermal fibroblasts is promoted and the expression of type I collagen and fibroblast growth factor is stimulated, and a good effect on skin turnover can be expected. In the epidermis, it stimulates the expression of type I collagen, keratin 10, and filaggrin by skin keratinocytes, which is effective in strengthening the skin barrier. Also, placenta extract is known to contain various antioxidant components, and it can be expected to bring about an anti-aging action by weakening the action of active oxygen generated by ultraviolet rays or the like.

  In addition, for example, an oral composition (Patent Document 1) characterized by containing a placenta extract, a collagen-derived component and a stress relieving agent, and an intestinal environment improving composition characterized by a combination of an oligosaccharide and a placenta extract ( Patent document 2), nerve cell activator containing placenta extract (patent document 3), collagen hydrolyzate, porcine placenta extract, lychee-derived low molecular weight polyphenol and ascorbic acid Health foods (Patent Document 4) and the like are known.

  In addition, many studies have been made on using a plant extract in combination with an external preparation for skin. However, plant extracts do not improve synergistically if they are used in combination, but the effects of the combined use, such as those that additively improve the effects and those that offset the effects, are unpredictable effects. Yes, there is a great need for the combined use of plant extracts that are more effective in smaller amounts.

Yasuhiko Komatsu et al., Fragrance Journal, Vol.44No.6, 47-54 (2016)

JP 2007-70316 A JP 2011-157335 A JP 2012-136448 A JP 2013-34423 A

  An object of the present invention is to provide a composition that synergistically improves the production of various growth factors in dermal fibroblasts and exhibits an anti-aging effect by using a placenta extract and a specific plant in combination. .

The present invention
(A) placenta extract;
(B) Provided is a composition containing one or more selected from crocus, merirot, valerian, sicon, and tencha.

  By using a placenta extract and a specific plant in combination, the composition of the present invention synergistically improves the expression of a growth factor or its receptor in dermal fibroblasts and exhibits a high anti-aging effect.

FIG. 1 is a diagram showing that the expression level of FGF1 (Fibroblast Growth Factor 1) gene is synergistically improved by using a placenta extract and a Merirot extract together. FIG. 2 is a diagram showing that the expression level of FGF2 (Fibroblast Growth Factor 2) gene is synergistically improved by using a placenta extract and a Merirot extract together. FIG. 3 is a diagram showing that the combined expression of CTGF (Connective Tissue Growth Factor) gene is synergistically improved by using a placenta extract and a Merirot extract together. FIG. 4 is a diagram showing that the combined expression of FGF1 (fibroblast growth factor 1) gene is synergistically improved by using placenta extract and crocus extract in combination. FIG. 5 is a diagram showing that the combined expression of FGF2 (Fibroblast Growth Factor 2) gene is synergistically improved by using placenta extract and crocus extract together. FIG. 6 is a diagram showing that the combined expression of CTGF (Connective Tissue Growth Factor) gene is synergistically improved by using a placenta extract and a crocus extract together. FIG. 7 is a diagram showing that the combined use of a placenta extract and a crocus extract synergistically improves the expression level of a VEGF (Vascular Endothelial Growth Factor) gene. FIG. 8 is a diagram showing that the combined expression of FGFR1 (Fibroblast Growth Factor Receptor 1) gene is synergistically improved by using a placenta extract and a crocus extract in combination. FIG. 9 is a diagram showing that the expression level of FGF1 (Fibroblast Growth Factor 1) gene is synergistically improved by using a placenta extract and an extract mixture of five kinds of plants in combination. . FIG. 10 is a diagram showing that the expression level of FGF2 (Fibroblast Growth Factor 2) gene is synergistically improved by using a placenta extract and an extract mixture of five kinds of plants in combination. . FIG. 11 is a diagram showing that the expression level of KGF (keratinocyte growth factor 1) gene is synergistically improved by using a placenta extract and an extract mixture of five kinds of plants in combination. . FIG. 12 shows that the combined expression of FGFR1 (Fibroblast Growth Factor Receptor 1) gene is synergistically improved by using a placenta extract and an extract mixture of five kinds of plants in combination. FIG.

  Hereinafter, modes for carrying out the present invention will be described.

  The composition of the present invention can be used for any application of cosmetics, quasi drugs, pharmaceuticals, and foods.

Placenta extract Origin of placenta to obtain placenta extract to be blended in the composition of the present invention is not particularly limited, but animals such as humans, cows, horses, pigs, sheep, goats, salmon, herring, sturgeon, roses, melons, Plants such as camellia and tulips are exemplified. Among these sources, it is preferable to use those derived from salmon, pigs, and plants from the viewpoint of commercial use. Moreover, there is a product already marketed as a placenta extract, and it may be used.

  Examples of salmon for obtaining salmon-derived placenta include white salmon, red salmon, silver salmon, rainbow trout, cherry salmon, and salmon. The placenta obtained from salmon uses an ovarian membrane. This ovarian membrane is a fish egg shell of the fish belonging to the moss, and can be obtained by washing only the shell after the fish egg is collected from the ovary. . In the composition of the present invention, a product obtained by pulverizing the ovarian membrane after washing with a mixer, a mortar, an emulsifier or the like may be used as it is, and a component extracted by treating the ovarian membrane with a proteolytic enzyme is used. It may be used.

  As a method for obtaining a porcine placenta extract, for example, it can be obtained by aseptically extracting with water from a pig placenta by freezing or thawing. Furthermore, you may use the component extracted by processing with a proteolytic enzyme.

  The concentration of the placenta extract in the composition of the present invention is not particularly limited, but is, for example, 0.0001 to 35 w / v%, preferably 0.01 to 35 w / v%. If the blending is less than 0.001 w / v%, the effect of the placenta extract may not be exhibited. Moreover, even if it mix | blends exceeding 35 w / v%, the effect may not be recognized but it is inefficient.

  The crocus compounded in the composition of the present invention is not particularly limited as long as it is a plant belonging to the genus Crocus, but crocus (Crocus Chrysanthus) or saffron (Crocus sativus) is preferably used. When using crocuses, it is preferable to use the root extract. When using saffron, it is preferable to use the pistil.

  Merilot (Melilotuis officinalis L.) to be blended in the composition of the present invention is a perennial plant belonging to the Leguminosae family (Leguminosae), and each part such as leaves, stems, flowers, fruits and whole plants can be used. It is preferable to use leaves alone or in combination with leaves and flowers.

  Valeriana fauriei blended in the composition of the present invention is a dicotyledonous plant of the genus Valeriana L., distributed from Hokkaido to Kyushu, Sakhalin, Korea, China, and slightly in the mountains. A perennial plant that grows in a wet grassland, and each part such as leaves, stems, flowers, roots, and whole grasses can be used, but roots and rhizomes are preferably used.

  As the silicon to be blended in the composition of the present invention, the root of Lithospermum officinale is used.

  Tencha blended into the composition of the present invention is a generic name for sweet tea made from leaves of Chinese vegetative tea that is different from botanical tea. suavissimus S. Lee, Raccoonaceae (Cow White Rattan) Oldenlandia hedyotidea, Beechaceae Tasuika (Taho 柯) or Tasui Sekikayo (Tahoishi 柯 葉) Lithocarpus polystachyus, Lauren Shukyu (Hairen embroidery bulb) Hydrangea aspera ssp Species such as strigosa are known, but in the present invention, it is preferable to use tencha obtained from leaves of the family Rosaceae.

  In the composition of the present invention, these plants can be used either raw or dried, or further extracted.

  In the composition of the present invention, the plant may be used alone or in combination of two or more. Furthermore, the anti-aging effect is synergistically improved by using these five types in combination.

  When using the plant used in this invention as an extract, the extraction method of the extract is described.

  In the present invention, each of the above plants may be subjected to extraction as it is, but considering the extraction efficiency, it is preferable to perform extraction after processing such as shredding, drying, and pulverization. Extraction is performed by immersing in an extraction solvent. In order to increase the extraction efficiency, stirring may be performed, or homogenization may be performed in an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 4 hours to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Polar organic solvents such as esters such as ethyl acetate and butyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used.

  The extract of the above-mentioned plant by the above-mentioned solvent can be contained in the composition according to the present invention as it is, but the concentrated and dried solid can be dissolved again in water or a polar solvent, or their skin physiological functions can be improved. It may be used after performing purification treatment such as decolorization, deodorization, desalting or the like within a range not impairing the action, or after fractionation treatment by column chromatography. For storage, it can be freeze-dried after purification and dissolved in a solvent before use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

  In particular, when used as a composition for transdermal use, the extract is used by being encapsulated in vesicles such as liposomes, microcapsules or the like, thereby enhancing percutaneous absorption and exhibiting a higher anti-aging effect.

  As a compounding quantity to the composition of the above-mentioned plant in this invention, Preferably it is 0.00001-5 weight%, Especially it is the range of 0.0001-1 weight%. If it is this range, when it mix | blends combining a specific plant, it will not affect the temporal stability of a formulation and a plant in a formulation, and can exhibit a higher effect.

  Examples of the method of applying the composition of the present invention include transdermal, oral, transmucosal, intradermal, and the like, and a suitable method may be appropriately selected according to the purpose.

  When it is used as a transdermal composition, the dosage form is arbitrary, and for example, it can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition to the essential components of the present invention, the transdermal composition contains oily ingredients, moisturizers, powders, pigments, emulsifiers, which are usually blended with pharmaceuticals, quasi drugs, and cosmetics as necessary. , Solubilizers, detergents, ultraviolet absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, and the like can be appropriately blended. Moreover, in the range which does not impair the effect of this invention, combined use with another moisturizer, a cell activator, an antioxidant, etc. is also possible.

  When it is set as an oral composition, the dosage form is arbitrary, For example, a powder form, a granular form, a tablet, a capsule, a jelly form, a liquid form etc. are illustrated.

  When preparing an oral composition, additives such as special surfactants are not essential, but if necessary, other known additives, excipients, etc. may be added and processed into an appropriate dosage form. May be. For example, in the case of a liquid agent, an antioxidant, a colorant, a flavoring agent, a surfactant, a plasticizer, a pH adjuster, etc. can be mixed to obtain an oral product such as a dry syrup agent or a liquid agent. If it is a solid agent, an excipient, a disintegrant, a binder, a lubricant, an antioxidant, a coating agent, a coloring agent, a corrigent, a surfactant, a plasticizer, etc. are mixed in a conventional manner. Granules, powders, capsules, tablets and the like can be produced.

  The composition of the present invention exhibits the effect of preventing aging by synergistically improving the expression of various growth factors or their receptor genes.

  EXAMPLES Hereinafter, the present invention will be specifically described with reference to examples, but the scope of the present invention is not limited thereby.

  First, the placenta extract used is described.

[Placenta extract 1]
As placenta extract 1, a placenta extract derived from salmon ovary membrane was used.

[Placenta extract 2]
As the placenta extract 2, a placenta extract obtained by hydrolyzing, filtering and drying a porcine-derived placenta was used as the placenta extract 2.

  Next, the preparation example of the extract of each used plant is shown.

[Crocus extract]
The roots of crocus were dried and then chopped and immersed in a 10% by mass 50% by weight aqueous ethanol solution, the filtrate after filtration was collected, and the solvent was distilled off. The obtained extract was dissolved in an aqueous ethanol solution, aged, and filtered again to obtain a crocus extract.

[Merirot extract]
Merirot leaves were dried and then chopped, dipped in 10 mass times 70 mass% 1,3-butylene glycol aqueous solution, the filtrate after filtration was collected, and the solvent was distilled off. The obtained extract was dissolved in a 70% by mass 1,3-butylene glycol aqueous solution, aged, and filtered again to obtain a Merirot extract.

[Valerian extract]
The roots and rhizomes of valerian were dried and then chopped, soaked in 10 parts by mass of purified water, the filtrate after filtration was collected, and the solvent was distilled off. The obtained extract was dissolved in a 20% by mass 1,3-butylene glycol aqueous solution, aged, and filtered again to obtain a valerian extract.

[Sicon extract]
The roots of Lithospermum officinale were dried and then chopped, dipped in a 10 mass times ethanol / BG mixture (9: 1), the filtrate after filtration was collected, and the solvent was distilled off. The obtained extract was dissolved in a glycerin / ethanol mixture (25:75) solution, aged, and filtered again to obtain a sicon extract.

[Tencha extract]
Tencha (Rubus suavissimus) leaves were dried and then chopped, soaked in 10 parts by mass of purified water, the filtrate after filtration was collected, and the solvent was distilled off. The obtained extract was dissolved in a 50 mass% 1,3-butylene glycol aqueous solution, aged, and filtered again to obtain a tencha extract.

[Extraction mixture]
A mixture obtained by mixing equal amounts of a crocus extract, a merirot extract, a valerian extract, a coconut extract and a tencha extract was used as an extraction mixture.

[Confirmation of synergy]
The combined use of placenta extract and a specific plant extract synergistically improves the expression of growth promoting factor gene or growth promoting factor receptor gene in fibroblasts. It was confirmed by the following procedure.

Human dermal fibroblasts were seeded in a 6-well plate at 3 × 10 ⁵ cells / well and cultured overnight in DMEM medium containing 5% FBS. The plant extract was replaced with a dissolved medium, and cultured in a 37 ° C., 5% CO ₂ incubator for 24 hours. Total RNA extraction from cells was prepared using QuickGene RNA cultured cell HC Kit S according to the protocol of Kurashiki Spinning Co., Ltd. After cDNA synthesis, gene expression was confirmed by real-time PCR using the primers shown in the table below. GAPDH shown in Table 3 below was used as an internal standard. Moreover, the addition amount to the culture medium of a plant extract was set to the addition amount at the time of single addition in the concentration which does not show cytotoxicity by single addition, and in the case of combined use, it was set to half the amount of addition at the time of single addition, respectively. In addition, placenta extract 1 was added in an amount that was the same as the control or the expression amount decreased by about 80% from the control, and half the amount of the single formulation was added in combination.

  As shown in FIGS. 1 to 3, by using placenta extract 1 and Merirot extract in combination, FGF1 (Fibroblast Growth Factor 1) gene, FGF2 (Fibroblast Growth Factor 2: The expression level of Fibroblast Growth Factor 2) gene and CTGF (Connective Tissue Growth Factor) gene increased synergistically.

  As shown in FIGS. 4 to 8, by using placenta extract 1 and crocus extract in combination, FGF1 gene, FGF2 gene, CTGF gene, VEGF (vascular endothelial growth factor) gene, FGFR1 (fiber Blast growth factor receptor 1: Fibroblast Growth Factor Receptor 1) The expression level of the gene increased synergistically.

  As shown in FIGS. 9 to 12, by using a placenta extract and an extract mixture of five kinds of plants in combination, FGF1 gene, FGF2 gene, KGF (keratinocyte growth factor 1) gene, FGFR1 gene The amount of expression increased synergistically.

  Next, examples in which the composition of the present invention is applied to an external preparation for skin will be described.

  As described in Table 2, the external preparation for skin using the placenta extract of the present invention and a specific plant extract was excellent in moisturizing feeling and firmness.

[Example 11] Drink agent
(1) Placenta extract 2 500mg
(2) Tencha extract 40mg
(3) Saffron extract 1mg
(4) Amount that makes citric acid pH 3.5
(5) Sodium citrate 1mg
(5) Thiamine nitrate 10mg
(6) Riboflavin sodium phosphate 10mg
(7) Pyridoxine hydrochloride 10mg
(8) Nicotinamide 100mg
(9) Anhydrous caffeine 100mg
(10) Aminoethylsulfonic acid 2000mg
(11) Glucose 5000mg
(12) Acesulfame potassium 30mg
(13) Sucralose 5mg
(14) Mixed fruit flavor 100mg
(Manufacturing method)
After the above components were dissolved in purified water, the pH was adjusted to 3.5, and purified water was further added to make up a total volume of 100 mL.

[Example 12] Jelly beverage
(1) Placenta extract 2 1400mg
(2) Tencha extract 50mg
(3) Saffron extract 1mg
(4) Amount that makes citric acid pH 3.5
(5) Sodium citrate 1mg
(6) Liquid sugar 3000mg
(7) Xanthan gum 3000mg
(8) Powdered agar 3000mg
(9) Carrageenan 500mg
(10) Gellan gum 500mg
(11) Flavor 100mg
(Manufacturing method)
After the above components were dissolved in purified water, the pH was adjusted to 3.5, and purified water was added to make the total amount 100 g.

Claims (1)

(A) placenta extract;
(B) A composition containing one or more selected from crocuses, merirot, valerian, sicon, tencha.

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