JP2018044935A - 化学分析装置、前処理装置及び化学分析方法 - Google Patents
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Abstract
【解決手段】検体に含まれる極性基を有する分子を捕捉する分子鋳型ポリマーを収容する前処理部60と、前処理部60に通液された検体に含まれる成分を定量する定量部65と、を有する化学分析装置600である。
【選択図】図1
Description
次に、分子鋳型ポリマーの製造方法について、さらに詳細に説明する。以下では、上記の手順に従い、化学分析を擾乱する測定阻害物質であるリン脂質を捕捉するための分子鋳型ポリマー微粒子を合成する場合について説明する。
実験例1では、リン脂質を捕捉する分子鋳型ポリマーを、S−ケトプロフェン(S−ketoprofen)をテンプレート分子として用いて合成した。
実施例1で合成した分子鋳型ポリマー(MIP)を用いて、リン脂質の除去能力を評価した。まず、事前実験として、種々のリン脂質濃度における吸光度を測定した。各リン脂質濃度における吸光度の測定値を表2に示す。これらの吸光度の測定値を、各リン脂質濃度に対してプロットして検量線を作成し、リン脂質濃度と吸光度との関係を算出した。なお、検量線の作製には、リン脂質として、1−Palmitoyl−2−oleoyl−sn−glycero−3−PC(1,2−POPC,1−Palmitoyl−2−oleoyl−sn−glycero−3−Phosphocholine)を用いた。図6に、リン脂質濃度と吸光度との関係を示す。図6に示すとおり、各リン脂質濃度に対する吸光度のプロットは、線形の関係となった。リン脂質濃度をx、吸光度をyとしたとき、これらの関係は、y=0.0324x+0.0165の計算式で近似され、相関係数R2=0.9894となった。
Claims (9)
- 検体に含まれる極性基を有する分子を捕捉する分子鋳型ポリマーを収容する前処理部と、
前記前処理部に通液された前記検体に含まれる成分を定量する定量部と、を有することを特徴とする化学分析装置。 - 前記前処理部は、前記分子鋳型ポリマーにより、前記極性基を有する分子として、リン脂質、リン脂質誘導体、リンタンパク質、リンペプチドからなる群から選択される少なくとも一つを捕捉することを特徴とする請求項1に記載の化学分析装置。
- 前記前処理部は、表面に前記分子鋳型ポリマーを有する分子鋳型ポリマー微粒子を収容することを特徴とする請求項1に記載の化学分析装置。
- 前記前処理部は、前記分子鋳型ポリマーとして、ケトプロフェン骨格を有する分子と特異的に結合可能な認識部位を有するポリマーを収容することを特徴とする請求項1に記載の化学分析装置。
- 前記定量部は、質量分析装置、液体クロマトグラフ又は液体クロマトグラフ質量分析装置であることを特徴とする請求項1に記載の化学分析装置。
- 検体に含まれる成分を定量分析する化学分析装置に使用される前処理装置であって、
前記前処理装置は、前記検体に含まれる極性基を有する分子を捕捉する分子鋳型ポリマーを備えており、該分子鋳型ポリマーにより、前記検体から前記極性基を有する分子を除去することを特徴とする前処理装置。 - 前記前処理装置は、前記分子鋳型ポリマーにより、前記極性基を有する分子として、リン脂質、リン脂質誘導体、リンタンパク質、リンペプチドからなる群から選択される少なくとも一つを除去することを特徴とする請求項6に記載の前処理装置。
- 極性基を有する分子を捕捉する分子鋳型ポリマーを収容する前処理部に検体を通液し、
前記前処理部に通液された前記検体に含まれる成分を定量分析することを特徴とする化学分析方法。 - 前記極性基を有する分子として、リン脂質、リン脂質誘導体、リンタンパク質、リンペプチドからなる群から選択される少なくとも一つを捕捉する前記分子鋳型ポリマーを収容する前記前処理部に検体を通液することを特徴とする請求項8に記載の化学分析方法。
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