JP2018038348A - Chemically-treated seed and its production method - Google Patents
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本発明は、薬剤処理種子及びその製造方法に関する。 The present invention relates to a drug-treated seed and a method for producing the same.
近年、育苗・定植の手間の省略が可能なことから、圃場に直接種子を播種する直播栽培が拡大してきている。 In recent years, direct sowing cultivation in which seeds are sown directly in the field has been expanded because it is possible to omit the trouble of raising seedlings and planting.
しかしながら、直播栽培は、発芽不良や生育不良が起こりやすいといった問題が知られており、その後の苗立ち（健全に生育したイネ２〜３葉期）にも大きな影響を及ぼすことから、さまざまな改善方法が提案されている（特許文献１、２）。 However, direct sowing cultivation is known to cause problems such as poor germination and poor growth, and it has a great impact on the subsequent seedling establishment (soundly growing rice 2-3 leaf stage). A method has been proposed (Patent Documents 1 and 2).
本発明は、出芽及び苗立ちが良好な種子を提供することを課題とする。 An object of the present invention is to provide seeds with good germination and seedling establishment.
本発明者らは、上記課題を解決すべく鋭意検討を行った結果、（Ａ）ジベレリン、（Ｂ）アブシシン酸、並びに（Ｃ）モリブデン及びモリブデン化合物から選ばれる少なくとも１つ、が、各々表面または内部に保持されている種子が、出芽及び苗立ちが良好になることを見出した。
すなわち本発明は、次の通りである。
［１］下記（Ａ）、（Ｂ）、及び（Ｃ）が、各々表面または内部に保持されている種子。
（Ａ）ジベレリン
（Ｂ）アブシシン酸
（Ｃ）モリブデン及びモリブデン化合物からなる群から選ばれる少なくとも１つ
［２］さらに鉄が表面に保持されている［１］に記載の種子。
［３］種子が稲の種子である［１］または［２］に記載の種子。
［４］［１］〜［３］のいずれか一項に記載の種子を、圃場に直接播種することを特徴とする植物の栽培方法。
［５］種子を、下記（ａ）、（ｂ）、及び（ｃ）に浸漬する工程を有する［１］〜［３］のいずれか一項に記載の種子の製造方法。
（ａ）ジベレリン溶液
（ｂ）アブシシン酸溶液
（ｃ）モリブデン及びモリブデン化合物からなる群から選ばれる少なくとも１つの溶液
［６］ジベレリンとアブシシン酸の濃度比（アブシシン酸／ジベレリン）が０．０００１〜１００の溶液、並びにジベレリンとモリブデン及びモリブデン化合物から選ばれる少なくとも１つの濃度比（モリブデン及びモリブデン化合物から選ばれる少なくとも１つ／ジベレリン）が０.０１〜１００００００の溶液を用いる、［５］に記載の種子の製造方法。
［７］種子を、ジベレリン溶液、及びアブシシン酸溶液に浸漬する工程を経た後、モリブデン及びモリブデン化合物から選ばれる少なくとも１つで被覆する工程を有する［１］〜［３］のいずれか一項に記載の種子の製造方法。
［８］ジベレリンとアブシシン酸の濃度比（アブシシン酸／ジベレリン）が０．０００１〜１００の溶液に浸漬する工程を経た後、０．１〜１０００μｍｏｌ／ｇ ｄｒｙ ｓｅｅｄの範囲で酸化モリブデン粉末を被覆する工程を有する［７］に記載の種子の製造方法。
As a result of intensive studies to solve the above problems, the present inventors have found that (A) gibberellin, (B) abscisic acid, and (C) at least one selected from molybdenum and a molybdenum compound, It has been found that the seeds held inside have good germination and seedling establishment.
That is, the present invention is as follows.
[1] Seeds in which the following (A), (B), and (C) are respectively held on the surface or inside.
(A) Gibberellin (B) Abscisic acid (C) At least one selected from the group consisting of molybdenum and a molybdenum compound [2] The seed according to [1], wherein iron is further retained on the surface.
[3] The seed according to [1] or [2], wherein the seed is a rice seed.
[4] A method for cultivating a plant, wherein the seed according to any one of [1] to [3] is directly sown in a field.
[5] The method for producing a seed according to any one of [1] to [3], including a step of immersing the seed in the following (a), (b), and (c).
(A) Gibberellin solution (b) Abscisic acid solution (c) At least one solution selected from the group consisting of molybdenum and molybdenum compounds [6] The concentration ratio (abscisic acid / gibberellin) of gibberellin and abscisic acid is 0.0001-100 And a solution having a concentration ratio of at least one selected from gibberellin and molybdenum and a molybdenum compound (at least one selected from molybdenum and a molybdenum compound / gibberellin) of 0.01 to 1,000,000 according to [5] Manufacturing method.
[7] The method according to any one of [1] to [3], including a step of covering the seed with at least one selected from molybdenum and a molybdenum compound after the step of immersing the seed in a gibberellin solution and an abscisic acid solution. A method for producing the seeds described.
[8] After a step of immersing in a solution having a concentration ratio of gibberellin and abscisic acid (abscisic acid / gibberellin) of 0.0001 to 100, the molybdenum oxide powder is coated in a range of 0.1 to 1000 μmol / g dry seed. The method for producing seeds according to [7], comprising a step.
本発明により、出芽及び苗立ちが良好な種子を提供することができる。 According to the present invention, it is possible to provide seeds with good germination and seedling establishment.
本発明に利用される種子としては、イネ科、マメ科、アブラナ科等の種子が挙げられる。これらのうち、一般的な栽培方法の中に、播種の前に水に浸漬して催芽させる過程のある作物が好適である。特に、イネ科イネ属イネ（Ｏｒｙｚａ ｓａｔｉｖａ）が好ましい。
本発明に利用される種子は、公知の方法で採種、調整されたものを用いることができる。
Examples of seeds used in the present invention include seeds of the grass family, legume family, cruciferous family and the like. Among these, crops having a process of germination by soaking in water before sowing are suitable among general cultivation methods. Particularly, Gramineae rice genus (Oryza sativa) is preferable.
The seeds used in the present invention can be seeds that have been seeded and adjusted by known methods.
ジベレリンとは、種子の発芽過程や細胞伸長、細胞分裂、花芽の分化・誘導、果実の肥大などに関与する植物ホルモンの一種であり、ジベレリンＡ１、ジベレリンＡ３、ジベレリンＡ４、およびジベレリンＡ７等が挙げられる。本発明においては、ジベレリンＡ３（ジベレリン酸）が好ましく、市販のジベレリンＡ３およびジベレリンＡ３を含む混合剤を利用することができる。
また、アブシシン酸とは、種子・芽の休眠、老化・器官脱離、気孔の閉鎖などに関与する植物ホルモンの一種であり、本発明においては、（Ｓ）−（＋）−アブシシン酸、（Ｒ）−（−）−アブシシン酸、２−トランス−アブシシン酸、（±）−アブシシン酸等市販の化合物を利用することができる。
Gibberellin is a kind of plant hormone involved in seed germination process, cell elongation, cell division, flower bud differentiation / induction, fruit hypertrophy, and the like. Gibberellin A1, gibberellin A3, gibberellin A4, gibberellin A7, and the like are listed. It is done. In the present invention, gibberellin A3 (gibberellic acid) is preferable, and a commercially available mixture containing gibberellin A3 and gibberellin A3 can be used.
Abscisic acid is a kind of plant hormone involved in seed / bud dormancy, aging / organ detachment, pore closure, etc. In the present invention, (S)-(+)-abscisic acid, ( Commercially available compounds such as R)-(−)-abscisic acid, 2-trans-abscisic acid, (±) -abscisic acid can be used.
本発明において、モリブデン及びモリブデン化合物から選ばれる少なくとも１つとは、モリブデン、酸化モリブデン（無水モリブデン酸）、モリブデン酸とその塩、モリブドリン酸（リンモリブデン酸）とその塩、モリブドケイ酸（ケイモリブデン酸）とその塩等をいう。モリブデン及びモリブデン化合物として安価で市販されているものとしては、モリブデン、酸化モリブデン、モリブデン酸、モリブデン酸カルシウム、モリブデン酸マグネシウム、モリブドリン酸アンモニウム（リンモリブデン酸アンモニウム）、モリブドリン酸カリウム（リンモリブデン酸カリウム）、モリブデン酸アンモニウム、モリブデン酸ナトリウム、モリブデン酸カリウム、モリブドリン酸、モリブドリン酸ナトリウム（リンモリブデン酸ナトリウム）、モリブドケイ酸が挙げられる。 In the present invention, at least one selected from molybdenum and molybdenum compounds is molybdenum, molybdenum oxide (molybdic anhydride), molybdic acid and its salt, molybdophosphoric acid (phosphomolybdic acid) and its salt, molybdosilicic acid (silicomolybdic acid) And its salts. Molybdenum, molybdenum oxide, molybdate, calcium molybdate, magnesium molybdate, ammonium molybdate (ammonium phosphomolybdate), potassium molybdophosphate (potassium phosphomolybdate) , Ammonium molybdate, sodium molybdate, potassium molybdate, molybdophosphoric acid, sodium molybdophosphate (sodium phosphomolybdate), molybdosilicate.
本発明の（Ａ）ジベレリン、（Ｂ）アブシシン酸、並びに（Ｃ）モリブデン及びモリブデン化合物から選ばれる少なくとも１つ、が、各々表面または内部に保持された種子（以下、本発明種子と記す）とは、種子に（Ａ）ジベレリン、（Ｂ）アブシシン酸、並びに（Ｃ）モリブデン及びモリブデン化合物から選ばれる少なくとも１つ、の各々を、浸漬処理により保持させたもの、あるいは、粉衣処理、吹付け処理、塗沫処理等の被覆処理により保持させたものをいう。（Ａ）ジベレリン、（Ｂ）アブシシン酸については浸漬処理、（Ｃ）モリブデン及びモリブデン化合物から選ばれる少なくとも１つについては浸漬処理あるいは被覆処理が好ましい。具体的には、以下の方法により製造することができる。
（１）種子を、下記（ａ）、（ｂ）、及び（ｃ）に浸漬する（以下、製造方法１と記す）。
（ａ）ジベレリン溶液
（ｂ）アブシシン酸溶液
（ｃ）モリブデン及びモリブデン化合物から選ばれる少なくとも１つの溶液
（２）種子を、ジベレリン溶液、及びアブシシン酸溶液に浸漬した後、モリブデン及びモリブデン化合物から選ばれる少なくとも１つで被覆する（以下、製造方法２と記す）。
(A) gibberellin of the present invention, (B) abscisic acid, and (C) at least one selected from molybdenum and a molybdenum compound, each of which is held on the surface or inside thereof (hereinafter referred to as the present invention seed); Is prepared by immersing each seed (A) gibberellin, (B) abscisic acid, and (C) at least one selected from molybdenum and a molybdenum compound, or a powder coating treatment, spraying It means the one held by a covering process such as a process or a smearing process. (A) Gibberellin and (B) Abscisic acid are preferably immersed, and (C) At least one selected from molybdenum and a molybdenum compound is preferably immersed or coated. Specifically, it can be produced by the following method.
(1) The seed is immersed in the following (a), (b), and (c) (hereinafter referred to as production method 1).
(A) Gibberellin solution (b) Abscisic acid solution (c) At least one solution selected from molybdenum and molybdenum compounds (2) After seeds are immersed in gibberellin solution and abscisic acid solution, selected from molybdenum and molybdenum compounds Cover with at least one (hereinafter referred to as production method 2).
製造方法１において、種子を（ａ）ジベレリン溶液、（ｂ）アブシシン酸溶液、並びに（ｃ）モリブデン及びモリブデン化合物から選ばれる少なくとも１つの溶液、に浸漬するとは、たとえば、ジベレリン溶液、アブシシン酸溶液、並びにモリブデン及びモリブデン化合物から選ばれる少なくとも１つの溶液、の各溶液に種子を浸漬することをいい、浸漬する順番は問わない。モリブデン及びモリブデン化合物から選ばれる少なくとも１つの溶液は、モリブデン及びモリブデン化合物が溶媒に分散している懸濁液でもよい。操作の簡便性から、ジベレリン、アブシシン酸、並びにモリブデン及びモリブデン化合物から選ばれる少なくとも１つの混合溶液に種子を浸漬することが好ましい。
ジベレリン、アブシシン酸、並びにモリブデン及びモリブデン化合物から選ばれる少なくとも１つ、を溶解または分散させる溶媒としては、通常水を用いる。水溶解度の低いアブシシン酸を使用する場合は、アブシシン酸をアセトン等の有機溶媒に溶解した後、水で所定濃度に希釈してもよい。ただし、有機溶媒の量は、種子の発芽に悪影響を及ぼさない範囲に調整する必要があり、混合液中の１％以下とすることが好ましい。
種子を浸漬する溶液中のジベレリンの濃度は、たとえば０．１〜１０００μｍｏｌ／Ｌ、好ましくは１〜１００μｍｏｌ／Ｌの範囲となるように調製する。同様に、アブシシン酸の濃度は、０．１〜１０μｍｏｌ／Ｌ、好ましくは０．４〜４μｍｏｌ／Ｌの範囲となるよう調製する。モリブデン及びモリブデン化合物から選ばれる少なくとも１つの濃度は、たとえば１０〜１０００００μｍｏｌ／Ｌ、好ましくは１００〜１０００００μｍｏｌ／Ｌの範囲となるように調製する。溶液中のジベレリンとアブシシン酸の濃度比（アブシシン酸／ジベレリン）は０．０００１〜１００、好ましくは０.００４〜４、ジベレリンとモリブデン及びモリブデン化合物から選ばれる少なくとも１つの濃度比（モリブデン及びモリブデン化合物から選ばれる少なくとも１つ／ジベレリン）は０.０１〜１００００００、好ましくは１〜１０００００である。
種子の浸漬方法、及び浸漬時間は、通常実施される「鉄コーティング湛水直播マニュアル２０１０」（山内稔 執筆、独立行政法人 農業・食品産業技術総合研究機構 近畿中国四国農業研究センター編、２０１０年３月）に準ずる。
In the production method 1, soaking seeds in (a) a gibberellin solution, (b) an abscisic acid solution, and (c) at least one solution selected from molybdenum and a molybdenum compound includes, for example, a gibberellin solution, an abscisic acid solution, In addition, the seed is immersed in each solution of at least one solution selected from molybdenum and a molybdenum compound, and the order of immersion is not limited. The at least one solution selected from molybdenum and a molybdenum compound may be a suspension in which molybdenum and a molybdenum compound are dispersed in a solvent. In view of ease of operation, it is preferable to immerse the seed in at least one mixed solution selected from gibberellin, abscisic acid, molybdenum and a molybdenum compound.
As a solvent for dissolving or dispersing gibberellin, abscisic acid, and at least one selected from molybdenum and a molybdenum compound, water is usually used. When abscisic acid having low water solubility is used, abscisic acid may be dissolved in an organic solvent such as acetone and then diluted to a predetermined concentration with water. However, the amount of the organic solvent needs to be adjusted within a range that does not adversely affect the germination of seeds, and is preferably 1% or less in the mixed solution.
The concentration of gibberellin in the solution in which the seeds are immersed is prepared, for example, in the range of 0.1 to 1000 μmol / L, preferably 1 to 100 μmol / L. Similarly, the concentration of abscisic acid is prepared in the range of 0.1 to 10 μmol / L, preferably 0.4 to 4 μmol / L. At least one concentration selected from molybdenum and a molybdenum compound is prepared, for example, in the range of 10 to 100,000 μmol / L, preferably 100 to 100,000 μmol / L. The concentration ratio of gibberellin to abscisic acid in the solution (abscisic acid / gibberellin) is 0.0001-100, preferably 0.004-4, at least one concentration ratio selected from gibberellin, molybdenum and molybdenum compounds (molybdenum and molybdenum compounds) At least one selected from (Gibberellin) is 0.01 to 1,000,000, preferably 1 to 100,000.
Seed soaking method and soaking time are as follows: “Iron-coated direct sowing manual 2010” written by Atsushi Yamauchi, National Institute of Agricultural and Food Research Kinki Chugoku Shikoku Agricultural Research Center, March 2010 Month).
製造方法２においては、種子をジベレリン溶液、及びアブシシン酸溶液に浸漬した後、モリブデン及びモリブデン化合物から選ばれる少なくとも１つで被覆する工程を有する。 種子をジベレリン溶液、及びアブシシン酸溶液に浸漬する方法は、ジベレリン溶液、アブシシン酸溶液の各溶液に浸漬してもよいし、ジベレリン、アブシシン酸の混合溶液に浸漬してもよい。次に、該浸漬工程を経た種子を、種子同士がまとまらず散らばる程度まで水分を除去し、モリブデン及びモリブデン化合物から選ばれる少なくとも１つで被覆する。モリブデン及びモリブデン化合物から選ばれる少なくとも１つで被覆する方法としては、種子表面にモリブデン及びモリブデン化合物から選ばれる少なくとも１つの粉末を、粉衣処理や吹付け処理する、あるいは、粉末を水等の溶媒で溶解または分散させた溶液で塗沫処理する等の被覆方法が挙げられる。
種子を浸漬する溶液中のジベレリン及びアブシシン酸の濃度、ジベレリンとアブシシン酸の濃度比は、製造方法１と同様である。
モリブデン及びモリブデン化合物から選ばれる少なくとも１つの被覆量は、例えば、酸化モリブデン粉末を用いた場合には０．１〜１０００μｍｏｌ／ｇ ｄｒｙ ｓｅｅｄ、好ましくは１０〜１００μｍｏｌ／ｇ ｄｒｙ ｓｅｅｄと、モリブデンが種子の発芽、及び発芽後の生育に悪影響をもたらさない範囲で保持させることができる。
The production method 2 includes a step of immersing the seed in a gibberellin solution and an abscisic acid solution and then coating the seed with at least one selected from molybdenum and a molybdenum compound. The method of immersing the seed in the gibberellin solution and the abscisic acid solution may be dipped in a gibberellin solution or abscisic acid solution, or in a mixed solution of gibberellin or abscisic acid. Next, moisture is removed from the seeds that have undergone the dipping process to such an extent that the seeds are not dispersed together and are coated with at least one selected from molybdenum and a molybdenum compound. As a method of coating with at least one selected from molybdenum and a molybdenum compound, at least one powder selected from molybdenum and a molybdenum compound is applied to the seed surface by a dressing treatment or a spraying treatment, or the powder is a solvent such as water. And a coating method such as a smearing treatment with a solution dissolved or dispersed in the above.
The concentrations of gibberellin and abscisic acid in the solution in which the seeds are immersed and the concentration ratio of gibberellin and abscisic acid are the same as in Production Method 1.
At least one coating amount selected from molybdenum and a molybdenum compound is, for example, 0.1 to 1000 μmol / g dry seed, preferably 10 to 100 μmol / g dry seed when molybdenum oxide powder is used, and molybdenum is a seed. It can be maintained within a range that does not adversely affect germination and growth after germination.
本発明種子は、さらに鉄が表面に保持されていてもよい。
鉄としては、還元鉄（Ｆｅ）、酸化鉄ＩＩＩ（Ｆｅ２Ｏ３）、四酸化三鉄（Ｆｅ３Ｏ４）、酸化鉄（ＩＩ）（ＦｅＯ）等の粉末を用いることができる。
鉄を種子表面に保持させる方法としては、特許文献１、２あるいは「鉄コーティング湛水直播マニュアル２０１０」（山内稔 執筆、独立行政法人 農業・食品産業技術総合研究機構 近畿中国四国農業研究センター編、２０１０年３月）に記載されている、公知の被覆方法を利用することができる。
The seed of the present invention may further have iron held on its surface.
As iron, powders of reduced iron (Fe), iron oxide III (Fe 2 O 3 ), triiron tetroxide (Fe 3 O 4 ), iron (II) oxide (FeO), and the like can be used.
As a method for holding iron on the seed surface, Patent Documents 1 and 2 or “Iron-coated direct sowing manual sowing manual 2010” (written by Satoshi Yamauchi, National Institute of Agricultural and Food Research, Kinki Shikoku Agricultural Research Center, Publicly known coating methods described in March 2010) can be used.
以上、ジベレリン、アブシシン酸、並びにモリブデン及びモリブデン化合物から選ばれる少なくとも１つを各々種子の表面または内部に保持させることにより、種子を圃場に直接播種した場合でも、出芽及び苗立ちが良好な種子を提供することができる。 As described above, by holding at least one selected from gibberellin, abscisic acid, molybdenum and a molybdenum compound on the surface or inside of the seed, seeds with good budding and seedling establishment can be obtained even when seeds are directly sown in the field. Can be provided.
以下、実施例により本発明をさらに具体的に説明するが、本発明はこれらに限定されるものではない。 EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited thereto.
調製例１
ジベレリン粉末（ジベレリンＡ３、有効成分３．１０％、協和発酵バイオ株式会社製）を所定濃度になるように水に溶解させ、ジベレリン水溶液を作製した。また、アセトン（和光純薬工業株式会社製）に、アブシシン酸粉末（（±）−アブシシン酸、有効成分９８．０％、シグマ アルドリッチ ジャパン合同会社製）を溶解させ、水で希釈することにより所定濃度のアブシシン酸水溶液（１％のアセトンを含む）を作製した。
次に、ジベレリン水溶液とアブシシン酸水溶液（１％のアセトンを含む）を混合し、ジベレリン０．３μｍｏｌ／Ｌ、アブシシン酸０．４μｍｏｌ／Ｌの混合液Ａを得た。得られた混合液Ａに、水稲の未消毒種子を浸漬し、積算温度４０℃に達するまで静置した。種子に対する混合液Ａの量としては、水稲種子を水で浸種する場合に通常使用される量（種子の２倍から３倍の量）を適用した。浸漬完了後、混合液Ａから種子を取り出し、種子１を得た。
Preparation Example 1
Gibberellin powder (gibberellin A3, active ingredient 3.10%, manufactured by Kyowa Hakko Bio Co., Ltd.) was dissolved in water to a predetermined concentration to prepare a gibberellin aqueous solution. In addition, abscisic acid powder ((±) -abscisic acid, active ingredient 98.0%, manufactured by Sigma-Aldrich Japan LLC) is dissolved in acetone (manufactured by Wako Pure Chemical Industries, Ltd.) and diluted with water. A concentration of abscisic acid aqueous solution (containing 1% acetone) was prepared.
Next, a gibberellin aqueous solution and an abscisic acid aqueous solution (containing 1% acetone) were mixed to obtain a mixed solution A of gibberellin 0.3 μmol / L and abscisic acid 0.4 μmol / L. Undisinfected seeds of paddy rice were soaked in the obtained mixed liquid A and allowed to stand until the integrated temperature reached 40 ° C. As the amount of the mixed solution A with respect to the seeds, the amount usually used when paddy rice seeds were soaked with water (2 to 3 times the amount of seeds) was applied. After completion of the immersion, seeds were taken out from the mixed solution A to obtain seeds 1.
調製例２
調製例１で得た混合液Ａに、酸化モリブデン粉末（有効成分９９．０％、和光純薬工業株式会社製一級）を１００００μｍｏｌ／Ｌとなるように添加し、撹拌して混合液Ｂを得た。得られた混合液Ｂに、水稲の未消毒種子を浸漬し、積算温度４０℃に達するまで静置した。種子に対する混合液Ｂの量としては、水稲種子を水で浸種する場合に通常使用される量（種子の２倍から３倍の量）を適用した。浸漬完了後、混合液Ｂから種子を取り出し、種子２を得た。
Preparation Example 2
Molybdenum oxide powder (active ingredient 99.0%, first grade manufactured by Wako Pure Chemical Industries, Ltd.) is added to the mixed solution A obtained in Preparation Example 1 at 10000 μmol / L and stirred to obtain a mixed solution B. It was. Undisinfected seeds of paddy rice were soaked in the obtained mixed solution B and allowed to stand until the accumulated temperature reached 40 ° C. As the amount of the mixed solution B with respect to the seeds, the amount normally used when paddy rice seeds were soaked with water (2 to 3 times the amount of seeds) was applied. After completion of the immersion, seeds were taken out from the mixed solution B to obtain seeds 2.
調製例３
水に調製例２で使用した酸化モリブデン粉末を１００００μｍｏｌ／Ｌとなるように添加し、撹拌して得られた懸濁液に、水稲の未消毒種子を浸漬し、積算温度４０℃に達するまで静置した。種子に対する懸濁液の量としては、水稲種子を水で浸種する場合に通常使用される量（種子の２倍から３倍の量）を適用した。浸漬完了後、懸濁液から種子を取り出し、種子３を得た。
Preparation Example 3
The molybdenum oxide powder used in Preparation Example 2 was added to water so that the concentration was 10,000 μmol / L, and the undisinfected seeds of paddy rice were immersed in the suspension obtained by stirring. I put it. As the amount of the suspension for the seeds, an amount usually used when paddy rice seeds were soaked with water (2 to 3 times the amount of seeds) was applied. After completion of the immersion, seeds were taken out from the suspension, and seeds 3 were obtained.
調製例４
水稲の未消毒種子を水（種子の２倍から３倍の量）に浸漬し、積算温度４０℃に達するまで静置した。浸漬完了後、水から種子を取り出し、種子４を得た。
Preparation Example 4
Undisinfected seeds of paddy rice were immersed in water (2 to 3 times the amount of seeds) and allowed to stand until an integrated temperature of 40 ° C. was reached. After completion of the immersion, the seeds were taken out from the water, and seeds 4 were obtained.
調製例５
調製例１で作製したジベレリン水溶液とアブシシン酸水溶液（１％のアセトンを含む）を混合し、ジベレリン１０μｍｏｌ／Ｌ、アブシシン酸４μｍｏｌ／Ｌの混合液Ｃを得た。得られた混合液Ｃに水稲の未消毒種子を浸漬し、積算温度４０℃に達するまで静置した。種子に対する混合液Ｃの量としては、水稲種子を水で浸種する場合に通常使用される量（種子の２倍から３倍の量）を適用した。浸漬が完了した種子について、種子同士がまとまらず散らばる程度まで水分を除去した後、調製例２で使用した酸化モリブデン粉末を乾燥種子１ｇに対して１００μｍｏｌ粉衣した。
Preparation Example 5
The gibberellin aqueous solution prepared in Preparation Example 1 and the abscisic acid aqueous solution (containing 1% acetone) were mixed to obtain a mixed solution C of gibberellin 10 μmol / L and abscisic acid 4 μmol / L. Undisinfected seeds of paddy rice were immersed in the obtained mixed liquid C and allowed to stand until the accumulated temperature reached 40 ° C. As the amount of the mixed solution C with respect to the seeds, the amount usually used when paddy rice seeds were soaked with water (2 to 3 times the amount of seeds) was applied. About the seed which immersion was completed, after removing a water | moisture content to such an extent that seeds were not gathered and scattered, the molybdenum oxide powder used in the preparation example 2 was dressed by 100 micromol with respect to 1 g of dry seeds.
調製例６
調製例５で得られた乾燥種子１ｋｇに対して、還元鉄（ＤＯＷＡ ＩＰ クリエイション株式会社製）２００ｇと焼石膏（吉野石膏販売株式会社製）を２０ｇ混合したものを、適度な水分を与えながら粉衣した。さらに焼石膏１０ｇを粉衣した後、バットの上に広げ、適宜水分を加えながら鉄を酸化させ、種子５を得た。
Preparation Example 6
A mixture of 200 g of reduced iron (DOWA IP Creation Co., Ltd.) and 20 g of calcined gypsum (Yoshino Gypsum Sales Co., Ltd.) mixed with 1 kg of the dried seed obtained in Preparation Example 5 while giving appropriate moisture. I wore it. Furthermore, after dressing 10 g of calcined gypsum, it was spread on a vat, and iron was oxidized while adding water as appropriate.
調製例７
調製例５で得られた混合液Ｃに水稲の未消毒種子を浸漬し、積算温度４０℃に達するまで静置した。種子に対する混合液Ｃの量としては、水稲種子を水で浸種する場合に通常使用される量（種子の２倍から３倍の量）を適用した。浸漬が完了した種子について、種子同士がまとまらず散らばる程度まで水分を除去した後、調製例６に記載の方法で鉄を種子表面に保持させ、種子６を得た。
Preparation Example 7
Undisinfected seeds of paddy rice were immersed in the mixed solution C obtained in Preparation Example 5 and allowed to stand until the accumulated temperature reached 40 ° C. As the amount of the mixed solution C with respect to the seeds, the amount usually used when paddy rice seeds were soaked with water (2 to 3 times the amount of seeds) was applied. About the seed which immersion was completed, after removing a water | moisture content to such an extent that seeds were not gathered and scattered, iron was hold | maintained on the seed surface by the method of the preparation example 6, and the seed 6 was obtained.
調製例８
調製例４で得られた種子について、種子同士がまとまらず散らばる程度まで水分を除去した後、調製例２で使用した酸化モリブデン粉末を乾燥種子１ｇに対して１００μｍｏｌ粉衣した。さらに調製例６に記載の方法で鉄を種子表面に保持させ、種子７を得た。
Preparation Example 8
About the seeds obtained in Preparation Example 4, after removing moisture to such an extent that the seeds were not scattered together, 100 μmol of the molybdenum oxide powder used in Preparation Example 2 was dressed on 1 g of dried seeds. Furthermore, iron was held on the seed surface by the method described in Preparation Example 6 to obtain seed 7.
調製例９
調製例４で得られた種子について、調製例６に記載の方法で鉄を種子表面に保持させ、種子８を得た。
Preparation Example 9
For the seed obtained in Preparation Example 4, iron was held on the seed surface by the method described in Preparation Example 6 to obtain seed 8.
調製例１０
調製例１で使用したジベレリン水溶液とアブシシン酸水溶液（１％のアセトンを含む）を混合し、ジベレリン１μｍｏｌ／Ｌ、アブシシン酸４μｍｏｌ／Ｌ混合液Dを得た。得られた混合液Dに水稲の未消毒種子を浸漬し、積算温度４０℃に達するまで静置した。種子に対する混合液Dの量としては、水稲種子を水で浸種する場合に通常使用される量（種子の２倍から３倍の量）を適用した。浸漬が完了した種子について、種子同士がまとまらず散らばる程度まで水分を除去した後、調製例２で使用した酸化モリブデン粉末を乾燥種子１ｇに対して１００μｍｏｌ粉衣した。さらに調製例６に記載の方法で鉄を種子表面に保持させ、種子９を得た。
Preparation Example 10
The gibberellin aqueous solution and the abscisic acid aqueous solution (containing 1% acetone) used in Preparation Example 1 were mixed to obtain a mixed solution D of gibberellin 1 μmol / L and abscisic acid 4 μmol / L. Undisinfected seeds of paddy rice were soaked in the obtained mixed solution D and allowed to stand until an integrated temperature of 40 ° C. was reached. As the amount of the mixed solution D with respect to the seeds, the amount usually used when paddy rice seeds were soaked with water (2 to 3 times the amount of seeds) was applied. About the seed which immersion was completed, after removing a water | moisture content to such an extent that seeds were not gathered and scattered, the molybdenum oxide powder used in the preparation example 2 was dressed by 100 micromol with respect to 1 g of dry seeds. Furthermore, iron was held on the seed surface by the method described in Preparation Example 6 to obtain seed 9.
調製例１１
調製例１６で得られた混合液Dに水稲の未消毒種子を浸漬し、積算温度４０℃に達するまで静置した。種子に対する混合液Dの量としては、水稲種子を水で浸種する場合に通常使用される量（種子の２倍から３倍の量）を適用した。浸漬が完了した種子について、種子同士がまとまらず散らばる程度まで水分を除去した後、調製例６に記載の方法で鉄を種子表面に保持させ、種子１０を得た。
Preparation Example 11
Undisinfected seeds of paddy rice were immersed in the mixed solution D obtained in Preparation Example 16, and allowed to stand until the accumulated temperature reached 40 ° C. As the amount of the mixed solution D with respect to the seeds, the amount usually used when paddy rice seeds were soaked with water (2 to 3 times the amount of seeds) was applied. About the seed which immersion was completed, after removing a water | moisture content to such an extent that seeds were not settled and scattered, iron was hold | maintained on the seed surface by the method of the preparation example 6, and the seed 10 was obtained.
試験例１
φ８ｃｍカップ（１/２０，０００ａ）にφ３ｍｍ篩下の水田土壌３００ｍｌを入れて代かき後、２日間静置した。湛水深を１ｃｍに調整し、２Ｍの硫酸アンモニウム水２００μｌを静かに添加した。数時間後、表１に記載の水稲種子を地表面に播種した。
播種１７日後に、出芽及び苗立ちが良好な健全苗を調査した結果を、表１に示した。
また、健全苗指数を下記より算出した。
健全苗指数（％）＝｛１−（１００−処理区の健全苗率（％））/（１００−無処理区の健全苗率（％））｝×１００
Test example 1
In a φ8 cm cup (1 / 20,000a), 300 ml of paddy field soil under a φ3 mm sieve was put, and left standing for 2 days. The water depth was adjusted to 1 cm, and 200 μl of 2M aqueous ammonium sulfate was gently added. Several hours later, paddy rice seeds listed in Table 1 were sown on the ground surface.
The results of investigating healthy seedlings with good emergence and seedling establishment after 17 days of sowing are shown in Table 1.
Moreover, the healthy seedling index was calculated from the following.
Healthy seedling index (%) = {1- (100-healthy seedling rate in treated area (%)) / (100-healthy seedling ratio in untreated area (%))} × 100
表中、ＧＡはジベレリン酸、ＡＢＡはアブシシン酸、ＭｏＯ３は酸化モリブデンを表す。（以下の表でも同様の表記とする）
In the table, GA represents gibberellic acid, ABA represents abscisic acid, and MoO 3 represents molybdenum oxide. (The same notation is used in the following table)
試験例２
表２及び表３に記載の種子を、試験例１と同様に調製したカップで、土壌表面から１．５ｃｍの深さのところに播種した。播種１３日後に健全苗を調査し、結果を表２及び表３に示した。また、健全苗指数は、試験例１の式により算出した。
Test example 2
Seeds listed in Tables 2 and 3 were sown in a cup prepared in the same manner as in Test Example 1 at a depth of 1.5 cm from the soil surface. Healthy seedlings were investigated 13 days after sowing, and the results are shown in Tables 2 and 3. Moreover, the healthy seedling index was calculated by the formula of Test Example 1.
本発明の種子は、出芽及び苗立ちが良好である。 The seeds of the present invention have good germination and seedling establishment.
Claims (8)
（Ａ）ジベレリン
（Ｂ）アブシシン酸
（Ｃ）モリブデン及びモリブデン化合物からなる群から選ばれる少なくとも１つ Seeds (A), (B), and (C) below are held on the surface or inside, respectively.
(A) Gibberellin (B) Abscisic acid (C) At least one selected from the group consisting of molybdenum and molybdenum compounds
（ａ）ジベレリン溶液
（ｂ）アブシシン酸溶液
（ｃ）モリブデン及びモリブデン化合物からなる群から選ばれる少なくとも１つの溶液 The method for producing seed according to any one of claims 1 to 3, further comprising a step of immersing the seed in the following (a), (b), and (c).
(A) gibberellin solution (b) abscisic acid solution (c) at least one solution selected from the group consisting of molybdenum and molybdenum compounds
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| JP6421299B1 (en) * | 2018-07-17 | 2018-11-14 | 憲照 曽我 | Coated seed for direct sowing and direct sowing cultivation method |
| JP2020010623A (en) * | 2018-07-17 | 2020-01-23 | 憲照 曽我 | Direct seeding coated seed and direct seeding culture method |
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