JP2017537645A5 - - Google Patents

Download PDF

Info

Publication number
JP2017537645A5
JP2017537645A5 JP2017532096A JP2017532096A JP2017537645A5 JP 2017537645 A5 JP2017537645 A5 JP 2017537645A5 JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017537645 A5 JP2017537645 A5 JP 2017537645A5
Authority
JP
Japan
Prior art keywords
barcode
epitope
unique binding
cells
cell origin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017532096A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017537645A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2015/067147 external-priority patent/WO2016100976A2/en
Publication of JP2017537645A publication Critical patent/JP2017537645A/ja
Publication of JP2017537645A5 publication Critical patent/JP2017537645A5/ja
Pending legal-status Critical Current

Links

JP2017532096A 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法 Pending JP2017537645A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462094919P 2014-12-19 2014-12-19
US201462094924P 2014-12-19 2014-12-19
US201462094917P 2014-12-19 2014-12-19
US62/094,924 2014-12-19
US62/094,917 2014-12-19
US62/094,919 2014-12-19
PCT/US2015/067147 WO2016100976A2 (en) 2014-12-19 2015-12-21 Methods for identifying multiple epitopes in selected sub-populations of cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020186442A Division JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Publications (2)

Publication Number Publication Date
JP2017537645A JP2017537645A (ja) 2017-12-21
JP2017537645A5 true JP2017537645A5 (OSRAM) 2019-01-17

Family

ID=56127888

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017532096A Pending JP2017537645A (ja) 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法
JP2020186442A Active JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2020186442A Active JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Country Status (5)

Country Link
US (3) US20180320241A1 (OSRAM)
EP (1) EP3234602B1 (OSRAM)
JP (2) JP2017537645A (OSRAM)
CN (1) CN107110849B (OSRAM)
WO (1) WO2016100976A2 (OSRAM)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
ES2687761T3 (es) 2011-01-31 2018-10-29 F. Hoffmann-La Roche Ag Métodos de identificación de múltiples epítopos en células
US11560585B2 (en) 2011-01-31 2023-01-24 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
CN114534806B (zh) 2014-04-10 2024-03-29 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3488239A1 (en) 2016-07-22 2019-05-29 Verily Life Sciences LLC Quantitative massively parallel proteomics
KR102522023B1 (ko) * 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622222D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
CN110291207A (zh) * 2017-01-27 2019-09-27 豪夫迈·罗氏有限公司 用于长程测序的条形码化dna
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
EP3447152A1 (en) * 2017-08-25 2019-02-27 Eawag, Swiss Federal Institute of Aquatic Science and Technology Accurate and massively parallel quantification of nucleic acid
ES2994431T3 (en) 2017-09-22 2025-01-23 Univ Washington In situ combinatorial labeling of cellular molecules
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN110462060B (zh) * 2017-12-08 2022-05-03 10X基因组学有限公司 用于标记细胞的方法和组合物
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
EP4545647A3 (en) 2018-05-03 2025-07-09 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US11039856B2 (en) 2018-05-10 2021-06-22 Verily Life Sciences Llc Surgical laparoscopic ports for implanting medical systems
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
EP4471156A3 (en) 2018-10-01 2025-02-26 Becton, Dickinson and Company Determining 5' transcript sequences
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
WO2020106906A1 (en) * 2018-11-21 2020-05-28 Avida Biomed, Inc. Methods for targeted nucleic acid library formation
US12460253B2 (en) * 2018-12-04 2025-11-04 Roche Sequencing Solutions, Inc. Spatially oriented quantum barcoding of cellular targets
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
ES2989967T3 (es) 2019-01-23 2024-11-28 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
SG10201904897UA (en) * 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
WO2021034791A1 (en) * 2019-08-19 2021-02-25 Inscripta, Inc. Optimizing high-throughput sequencing capacity
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
WO2021113290A1 (en) 2019-12-03 2021-06-10 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US20230054899A1 (en) * 2020-01-14 2023-02-23 William Marsh Rice University High throughput genetic barcoding and analysis methods
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
EP4162069A1 (en) * 2020-06-08 2023-04-12 F. Hoffmann-La Roche AG Method of single-cell analysis of multiple samples
US20230340578A1 (en) * 2020-07-08 2023-10-26 Roche Sequencing Solutions, Inc. Spatial analysis of multiple targets in tissue samples
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20250129405A1 (en) * 2021-09-08 2025-04-24 Becton, Dickinson And Company Non-sequencing pcr-based method for detection of antibody-conjugated oligonucleotides

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
WO2001056955A1 (en) * 2000-02-03 2001-08-09 Nanoscale Combinatorial Synthesis, Inc. Nonredundant split/pool synthesis of combinatorial libraries
SE516272C2 (sv) * 2000-02-18 2001-12-10 Ulf Landegren Metoder och kit för analytdetektion mha proximitets-probning
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
WO2009023107A1 (en) * 2007-08-10 2009-02-19 Peter Oliver Krutzik Biological encoding of large numbers of cells
US8853185B2 (en) * 2008-04-09 2014-10-07 Cornell University Coferons and methods of making and using them
GB0816976D0 (en) * 2008-09-16 2008-10-22 Natural Environment Res Council Protein
US8481698B2 (en) * 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
ES2687761T3 (es) * 2011-01-31 2018-10-29 F. Hoffmann-La Roche Ag Métodos de identificación de múltiples epítopos en células
JP6153874B2 (ja) * 2011-02-09 2017-06-28 ナテラ, インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
EP4450644A3 (en) * 2012-08-08 2025-01-08 F. Hoffmann-La Roche AG Increasing dynamic range for identifying multiple epitopes in cells
EP4481048A3 (en) * 2012-12-17 2025-02-26 President and Fellows of Harvard College Rna-guided human genome engineering

Similar Documents

Publication Publication Date Title
JP2017537645A5 (OSRAM)
US20200248235A1 (en) Methods to profile molecular complexes or single cells via by using proximity bar-coding dependant barcoding
CN111032881B (zh) 核酸的精确和大规模平行定量
Song et al. Single cell transcriptomics: moving towards multi-omics
JP2019518434A5 (OSRAM)
JP2020511629A5 (OSRAM)
US9850482B2 (en) Heterologous DNA barcoding method
JP2018529314A5 (OSRAM)
JP2018516541A5 (OSRAM)
CN107208157A (zh) 用于条形编码核酸以用于测序的方法和组合物
WO2012159564A1 (zh) 甲基化高通量检测方法
JP2019520037A5 (OSRAM)
US10435736B2 (en) Target region enrichment method based on multiplex PCR, and reagent
CN106498504A (zh) 基于多重pcr的二代测序建库技术
JP7761619B2 (ja) 核酸の正確な並行検出及び定量のための方法
CN113557300A (zh) 核酸序列、rna目标区域测序文库的构建方法及应用
JP2022502343A (ja) 肝癌によく見られた複数の変異を同時に検出するctDNAライブラリーの構築及びシークエンシングデータの分析の方法
TWI864378B (zh) 用於準確的平行定量核酸的高靈敏度方法
KR20210084441A (ko) 어레이 상에서 인덱스 및 바코드를 사용하여 리간드를 확인하기 위한 방법 및 조성물
CN108823296A (zh) 一种检测核酸样本污染的方法、试剂盒及应用
JP2009502190A5 (OSRAM)
US9499814B2 (en) Methods for identifying drug effects on a cell by determining changes in the cell's spliced message profile
CN103667267B (zh) 用于与kras基因杂交的dna探针库及采用其富集kras基因片段的方法
Tost et al. Molecular techniques for DNA methylation studies
FI4077713T3 (fi) Menetelmä ja kitti kokogenomiamplifikaatioon ja biologisessa näytteessä olevien kohdemolekyylien analysointiin