JP2017534285A5 - - Google Patents
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- JP2017534285A5 JP2017534285A5 JP2017523864A JP2017523864A JP2017534285A5 JP 2017534285 A5 JP2017534285 A5 JP 2017534285A5 JP 2017523864 A JP2017523864 A JP 2017523864A JP 2017523864 A JP2017523864 A JP 2017523864A JP 2017534285 A5 JP2017534285 A5 JP 2017534285A5
- Authority
- JP
- Japan
- Prior art keywords
- scaffold
- compatible end
- end element
- vector
- crispr guide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462073126P | 2014-10-31 | 2014-10-31 | |
| US62/073,126 | 2014-10-31 | ||
| US201562166302P | 2015-05-26 | 2015-05-26 | |
| US62/166,302 | 2015-05-26 | ||
| PCT/US2015/058304 WO2016070037A2 (en) | 2014-10-31 | 2015-10-30 | Massively parallel combinatorial genetics for crispr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017534285A JP2017534285A (ja) | 2017-11-24 |
| JP2017534285A5 true JP2017534285A5 (OSRAM) | 2018-12-13 |
| JP6788584B2 JP6788584B2 (ja) | 2020-11-25 |
Family
ID=54884363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017523864A Active JP6788584B2 (ja) | 2014-10-31 | 2015-10-30 | Ｃｒｉｓｐｒについての超並列コンビナトリアル遺伝学 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20190100769A1 (OSRAM) |
| EP (2) | EP3212789B1 (OSRAM) |
| JP (1) | JP6788584B2 (OSRAM) |
| CN (1) | CN107429246B (OSRAM) |
| DK (1) | DK3212789T3 (OSRAM) |
| TW (1) | TWI716367B (OSRAM) |
| WO (1) | WO2016070037A2 (OSRAM) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| DE212015000061U1 (de) | 2014-02-11 | 2017-09-03 | The Regents Of The University Of Colorado, A Body Corporate | CRISPR-ermöglichtes Multiplex Genom Engineering |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US10900034B2 (en) | 2014-12-03 | 2021-01-26 | Agilent Technologies, Inc. | Guide RNA with chemical modifications |
| EP3280803B1 (en) | 2015-04-06 | 2021-05-26 | The Board of Trustees of the Leland Stanford Junior University | Chemically modified guide rnas for crispr/cas-mediated gene regulation |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and uses thereof |
| BR112018013679A2 (pt) | 2016-01-11 | 2019-01-22 | Univ Leland Stanford Junior | proteínas quiméricas e métodos de regulação de expressão gênica |
| MX2018008345A (es) | 2016-01-11 | 2018-12-06 | Univ Leland Stanford Junior | Proteínas quiméricas y métodos de inmunoterapia. |
| JP2019514377A (ja) * | 2016-04-26 | 2019-06-06 | マサチューセッツ インスティテュート オブ テクノロジー | 拡張可能なリコンビナーゼカスケード |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| WO2017223538A1 (en) | 2016-06-24 | 2017-12-28 | The Regents Of The University Of Colorado, A Body Corporate | Methods for generating barcoded combinatorial libraries |
| WO2018005691A1 (en) * | 2016-06-29 | 2018-01-04 | The Regents Of The University Of California | Efficient genetic screening method |
| KR102827276B1 (ko) | 2016-08-03 | 2025-07-01 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| JP7308143B2 (ja) | 2016-08-19 | 2023-07-13 | ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ | Ｄｎａメチル化の編集方法 |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018049073A1 (en) | 2016-09-07 | 2018-03-15 | Flagship Pioneering, Inc. | Methods and compositions for modulating gene expression |
| KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018111944A1 (en) | 2016-12-12 | 2018-06-21 | Whitehead Institute For Biomedical Research | Regulation of transcription through ctcf loop anchors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| AU2017385611B2 (en) * | 2016-12-29 | 2023-11-02 | Johann Wolfgang Goethe-Universität Frankfurt am Main | Method for generating higher order genome editing libraries |
| FI3565891T3 (fi) | 2017-01-09 | 2023-07-04 | Whitehead Inst Biomedical Res | Menetelmiä geeniekspression muuttamiseksi häiritsemällä säätelysilmukoita muodostavia transkriptiotekijämultimeerejä |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| WO2018145041A1 (en) * | 2017-02-06 | 2018-08-09 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
| EP3585160A2 (en) | 2017-07-31 | 2020-01-01 | Regeneron Pharmaceuticals, Inc. | Crispr reporter non-human animals and uses thereof |
| BR112020001364A2 (pt) | 2017-07-31 | 2020-08-11 | Regeneron Pharmaceuticals, Inc. | métodos para testar e modificar a capacidade de uma crispr/cas nuclease. |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
| US11547614B2 (en) | 2017-10-31 | 2023-01-10 | The Broad Institute, Inc. | Methods and compositions for studying cell evolution |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| EP3820495A4 (en) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | RNA PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES |
| WO2020033585A1 (en) * | 2018-08-07 | 2020-02-13 | The Broad Institute, Inc. | Methods for combinatorial screening and use of therapeutic targets thereof |
| CN112955549B (zh) | 2018-09-19 | 2024-12-20 | 香港大学 | 改进的高通量组合基因修饰系统和优化的Cas9酶变体 |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| JP7144618B2 (ja) * | 2018-12-20 | 2022-09-29 | 北京大学 | バーコード付きガイドｒｎａ構築体を使用する効率的な遺伝子スクリーニングのための組成物及び方法 |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| JP7657726B2 (ja) | 2019-03-19 | 2025-04-07 | ザ ブロード インスティテュート，インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| JP2022548399A (ja) | 2019-09-23 | 2022-11-18 | オメガ セラピューティクス， インコーポレイテッド | 肝細胞核因子４－アルファ（ＨＮＦ４α）遺伝子発現をモジュレートするための組成物および方法 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN110652589A (zh) * | 2019-10-12 | 2020-01-07 | 中国人民解放军陆军军医大学 | Gasc1抑制剂在制备治疗肝癌的药物中的应用 |
| US20230002756A1 (en) * | 2019-12-12 | 2023-01-05 | The Trustees Of The University Of Pennsylvania | High Performance Platform for Combinatorial Genetic Screening |
| CN115397996B (zh) * | 2020-04-16 | 2024-11-08 | 香港大学 | 用于分析靶相互作用的三元组合crispr筛选系统及其方法 |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| US20240110175A1 (en) * | 2021-01-19 | 2024-04-04 | The Board Of Trustees Of The Leland Stanford Junior University | Composition and method for high-multiplexed genome engineering using synthetic crispr arrays |
| WO2022195582A1 (en) * | 2021-03-15 | 2022-09-22 | G.T.A.I Innovation Ltd. | A method and apparatus for lab tests |
| CN113599522B (zh) * | 2021-08-03 | 2022-09-20 | 深圳市北科生物科技有限公司 | Kdm6作为靶标在制备用于提高早期神经外胚层分化效率的药物中的应用 |
| AU2022343300A1 (en) | 2021-09-10 | 2024-04-18 | Agilent Technologies, Inc. | Guide rnas with chemical modification for prime editing |
| CN116019811B (zh) * | 2023-02-07 | 2024-04-12 | 华中科技大学协和深圳医院 | Gsk-j4用于制备抗革兰氏阳性菌感染药物中的应用 |
| CN118425525B (zh) * | 2024-03-13 | 2025-08-22 | 郑州大学第一附属医院 | Kdm5b在肾纤维化治疗中的应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2005240741A1 (en) * | 2004-05-10 | 2005-11-17 | Basf Plant Science Gmbh | Methods for assembling multiple expression constructs |
| JP5478262B2 (ja) * | 2007-12-28 | 2014-04-23 | 田辺三菱製薬株式会社 | 抗癌剤 |
| EP2569434B1 (en) * | 2010-05-14 | 2019-09-04 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for treating leukemia and related disorders |
| AR084070A1 (es) * | 2010-12-02 | 2013-04-17 | Constellation Pharmaceuticals Inc | Inhibidores del bromodominio y usos de los mismos |
| LT3401400T (lt) * | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
| CN103516916B (zh) | 2012-06-28 | 2018-03-27 | 中兴通讯股份有限公司 | 拨号方法及装置 |
| EP2867361B1 (en) * | 2012-06-29 | 2017-11-01 | Massachusetts Institute of Technology | Massively parallel combinatorial genetics |
| DK2903968T3 (en) * | 2012-10-02 | 2017-01-30 | Gilead Sciences Inc | INHIBITORS OF HISTON DEMETHYLASES |
| WO2014080290A2 (en) * | 2012-11-21 | 2014-05-30 | Rvx Therapeutics Inc. | Cyclic amines as bromodomain inhibitors |
| CN103833671B (zh) * | 2012-11-27 | 2016-12-21 | 中国科学院上海药物研究所 | 一类二氢噻唑酮类化合物及其药物组合物和用途 |
| DK3138912T3 (en) * | 2012-12-06 | 2019-01-21 | Sigma Aldrich Co Llc | CRISPR-BASED RE-MODIFICATION AND REGULATION |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| JP2016501531A (ja) * | 2012-12-12 | 2016-01-21 | ザ・ブロード・インスティテュート・インコーポレイテッド | 配列操作および治療適用のための系、方法および組成物の送達、エンジニアリングおよび最適化 |
| WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| US20140315985A1 (en) * | 2013-03-14 | 2014-10-23 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| EP2978757B1 (en) | 2013-03-27 | 2017-02-08 | Boehringer Ingelheim International GmbH | Indolinone analogues as brd4 inhibitors |
| JP6738729B2 (ja) * | 2013-06-17 | 2020-08-12 | ザ・ブロード・インスティテュート・インコーポレイテッド | 分裂終了細胞の疾患および障害をターゲティングおよびモデリングするための系、方法および組成物の送達、エンジニアリングおよび最適化 |
| BR112016013213A2 (pt) * | 2013-12-12 | 2017-12-05 | Massachusetts Inst Technology | administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para visar distúrbios e doenças usando componentes de administração de partículas |
| BR112016013207A2 (pt) * | 2013-12-12 | 2017-09-26 | Massachusetts Inst Technology | administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para o hbv e distúrbios e doenças virais |
| KR102805572B1 (ko) * | 2013-12-12 | 2025-05-09 | 더 브로드 인스티튜트, 인코퍼레이티드 | 게놈 편집을 위한 ｃｒｉｓｐｒ-ｃａｓ 시스템 및 조성물의 전달, 용도 및 치료적 응용 |
| WO2015095501A1 (en) * | 2013-12-18 | 2015-06-25 | Onn Brandman | Pooled method for high throughput screening of trans factors affecting rna levels |
| CN106170550A (zh) * | 2014-04-03 | 2016-11-30 | 麻省理工学院 | 用于产生导引rna的方法和组合物 |
| US20170044107A1 (en) * | 2014-04-28 | 2017-02-16 | The J. David Gladstone Institutes | Inhibitors of JMJD2C as Anticancer Agents |
| AU2015301746A1 (en) * | 2014-08-13 | 2017-02-16 | Celgene Corporation | Combination therapy for treating cancer |
| WO2016049024A2 (en) * | 2014-09-24 | 2016-03-31 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling competition of multiple cancer mutations in vivo |
-
2015
- 2015-10-30 EP EP15813148.2A patent/EP3212789B1/en active Active
- 2015-10-30 DK DK15813148.2T patent/DK3212789T3/da active
- 2015-10-30 TW TW104135960A patent/TWI716367B/zh active
- 2015-10-30 WO PCT/US2015/058304 patent/WO2016070037A2/en not_active Ceased
- 2015-10-30 CN CN201580065962.8A patent/CN107429246B/zh active Active
- 2015-10-30 JP JP2017523864A patent/JP6788584B2/ja active Active
- 2015-10-30 US US15/521,931 patent/US20190100769A1/en not_active Abandoned
- 2015-10-30 EP EP20167714.3A patent/EP3708155A1/en not_active Withdrawn
-
2021
- 2021-06-03 US US17/338,027 patent/US20210310022A1/en not_active Abandoned
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