JP2017518750A5 - - Google Patents
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- Publication number
- JP2017518750A5 JP2017518750A5 JP2016571250A JP2016571250A JP2017518750A5 JP 2017518750 A5 JP2017518750 A5 JP 2017518750A5 JP 2016571250 A JP2016571250 A JP 2016571250A JP 2016571250 A JP2016571250 A JP 2016571250A JP 2017518750 A5 JP2017518750 A5 JP 2017518750A5
- Authority
- JP
- Japan
- Prior art keywords
- polymerase
- amino acid
- acid sequence
- functionally equivalent
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 125000003275 alpha amino acid group Chemical group 0.000 claims 25
- 230000035772 mutation Effects 0.000 claims 17
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 16
- 238000006467 substitution reaction Methods 0.000 claims 15
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 9
- 108020004511 Recombinant DNA Proteins 0.000 claims 6
- 102220532233 Paraneoplastic antigen Ma3_L408A_mutation Human genes 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 108020004634 Archaeal DNA Proteins 0.000 claims 1
- 241000203353 Methanococcus Species 0.000 claims 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 claims 1
- 102220477099 Protein C-ets-1_T514A_mutation Human genes 0.000 claims 1
- 241000205160 Pyrococcus Species 0.000 claims 1
- 241000205188 Thermococcus Species 0.000 claims 1
- 102220010377 rs104894722 Human genes 0.000 claims 1
- 102220127832 rs886044912 Human genes 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462018470P | 2014-06-27 | 2014-06-27 | |
| US62/018,470 | 2014-06-27 | ||
| PCT/US2015/037785 WO2015200693A1 (en) | 2014-06-27 | 2015-06-25 | Modified polymerases for improved incorporation of nucleotide analogues |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020079698A Division JP2020127415A (ja) | 2014-06-27 | 2020-04-28 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017518750A JP2017518750A (ja) | 2017-07-13 |
| JP2017518750A5 true JP2017518750A5 (enExample) | 2018-08-02 |
| JP6737710B2 JP6737710B2 (ja) | 2020-08-12 |
Family
ID=53716556
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016571250A Active JP6737710B2 (ja) | 2014-06-27 | 2015-06-25 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
| JP2020079698A Pending JP2020127415A (ja) | 2014-06-27 | 2020-04-28 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
| JP2021196287A Pending JP2022033882A (ja) | 2014-06-27 | 2021-12-02 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020079698A Pending JP2020127415A (ja) | 2014-06-27 | 2020-04-28 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
| JP2021196287A Pending JP2022033882A (ja) | 2014-06-27 | 2021-12-02 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US9765309B2 (enExample) |
| EP (2) | EP3161154B1 (enExample) |
| JP (3) | JP6737710B2 (enExample) |
| CN (1) | CN106536728A (enExample) |
| AU (2) | AU2015279794B2 (enExample) |
| DK (1) | DK3161154T3 (enExample) |
| ES (2) | ES3060591T3 (enExample) |
| PL (1) | PL3161154T3 (enExample) |
| PT (1) | PT3161154T (enExample) |
| WO (1) | WO2015200693A1 (enExample) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3161154B1 (en) * | 2014-06-27 | 2020-04-15 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| US9677057B2 (en) | 2014-09-30 | 2017-06-13 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| US10662413B2 (en) * | 2016-11-01 | 2020-05-26 | Personal Genomics, Inc. | Recombinant DNA polymerase for improved incorporation of nucleotide analogues |
| WO2018126470A1 (zh) * | 2017-01-09 | 2018-07-12 | 深圳华大智造科技有限公司 | 重组dna聚合酶 |
| EP3580351A1 (en) | 2017-02-13 | 2019-12-18 | Qiagen Sciences, LLC | Polymerase enzyme from 9°n |
| WO2018148724A1 (en) * | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from pyrococcus furiosus |
| EP3580350B1 (en) | 2017-02-13 | 2020-11-18 | Qiagen Sciences, LLC | Polymerase enzyme from pyrococcus furiosus |
| AU2018259202B2 (en) | 2017-04-23 | 2022-03-24 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
| ES2937929T3 (es) | 2017-04-23 | 2023-04-03 | Illumina Inc | Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácido nucleico indexadas |
| US10975430B2 (en) | 2017-04-23 | 2021-04-13 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
| CN108795900B (zh) * | 2017-04-27 | 2021-02-02 | 深圳华大智造科技股份有限公司 | Dna聚合酶及其制备方法 |
| CN117802065A (zh) * | 2018-09-03 | 2024-04-02 | 深圳华大生命科学研究院 | 聚合活性提高的重组型kod聚合酶 |
| EP3850110A4 (en) | 2018-09-11 | 2022-06-08 | Singular Genomics Systems, Inc. | MODIFIED ARCHAEAL POLYMERASES OF THE B FAMILY |
| CA3103719A1 (en) | 2018-10-31 | 2020-05-07 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| CA3103739A1 (en) * | 2018-12-05 | 2020-06-11 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| US11293061B2 (en) * | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
| US11512295B2 (en) | 2019-09-12 | 2022-11-29 | Singular Genomics Systems, Inc. | Modified thermoccocus polymerases |
| WO2021058438A1 (en) | 2019-09-23 | 2021-04-01 | Dna Script | Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides |
| US11591629B2 (en) * | 2019-12-23 | 2023-02-28 | Cheng-Yao Chen | Method and kit for template-independent nucleic acid synthesis |
| US11034942B1 (en) | 2020-02-27 | 2021-06-15 | Singular Genomics Systems, Inc. | Modified pyrococcus polymerases and uses thereof |
| US20230203592A1 (en) | 2020-05-05 | 2023-06-29 | Akershus Universitetssykehus Hf | Compositions and methods for characterizing bowel cancer |
| CN116547387A (zh) * | 2020-10-21 | 2023-08-04 | 深圳华大生命科学研究院 | 重组型kod聚合酶 |
| IL309440A (en) * | 2021-06-18 | 2024-02-01 | Element Biosciences Inc | Engineered polymerases |
| IL309368A (en) | 2021-06-29 | 2024-02-01 | Agilent Technologies Inc | polymerase mutants and use with 3'-OH UNBLOCKED REVERSIBLE TERMINATORS |
| US12077789B2 (en) | 2021-08-14 | 2024-09-03 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| TWI869710B (zh) | 2021-09-29 | 2025-01-11 | 呈堯 陳 | 用於無模板ｒｎａ合成的核酸聚合酶變體、試劑套組及方法 |
| WO2024039652A1 (en) | 2022-08-16 | 2024-02-22 | Aldevron, L.L.C. | Cell-free method of producing synthetic circular nucleic acid |
| US20240141427A1 (en) | 2022-09-30 | 2024-05-02 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| WO2024155578A1 (en) * | 2023-01-16 | 2024-07-25 | The Regents Of The University Of California | Engineered polymerase for threose nucleic acid synthesis |
| CN116286720A (zh) * | 2023-03-29 | 2023-06-23 | 赛纳生物科技(北京)有限公司 | 一种dna聚合酶 |
| WO2025260380A1 (zh) * | 2024-06-21 | 2025-12-26 | 华大工程生物学长荡湖研究所 | 重组型kod聚合酶 |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0450060A1 (en) | 1989-10-26 | 1991-10-09 | Sri International | Dna sequencing |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US5948666A (en) * | 1997-08-06 | 1999-09-07 | Diversa Corporation | Isolation and identification of polymerases |
| US20050191698A1 (en) | 1999-04-20 | 2005-09-01 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
| US6492161B1 (en) * | 1999-06-02 | 2002-12-10 | Prokaria Ltd. | Bacteriophage RM 378 of a thermophilic host organism |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US20030228616A1 (en) * | 1999-10-29 | 2003-12-11 | Stratagene | DNA polymerase mutants with reverse transcriptase activity |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| EP2607369B1 (en) | 2002-08-23 | 2015-09-23 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
| US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US20060062531A1 (en) | 2004-09-17 | 2006-03-23 | Stephen Turner | Fabrication of optical confinements |
| US20070048748A1 (en) * | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
| GB0509508D0 (en) * | 2005-05-10 | 2005-06-15 | Solexa Ltd | Improved polymerases |
| US8623628B2 (en) | 2005-05-10 | 2014-01-07 | Illumina, Inc. | Polymerases |
| US20070022459A1 (en) | 2005-07-20 | 2007-01-25 | Gaebel Thomas M Jr | Method and apparatus for boundary-based network operation |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| CA2633524A1 (en) | 2005-12-22 | 2007-07-05 | Pacific Biosciences Of California, Inc. | Polymerases for nucleotide analogue incorporation |
| WO2007123744A2 (en) | 2006-03-31 | 2007-11-01 | Solexa, Inc. | Systems and devices for sequence by synthesis analysis |
| WO2008006781A2 (en) | 2006-07-14 | 2008-01-17 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
| US8343746B2 (en) | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| EP2639578B1 (en) | 2006-12-14 | 2016-09-14 | Life Technologies Corporation | Apparatus for measuring analytes using large scale fet arrays |
| US20110045489A1 (en) * | 2008-04-22 | 2011-02-24 | New England Biolabs, Inc. | Polymerases for Incorporating Modified Nucleotides |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US8481685B2 (en) * | 2008-11-03 | 2013-07-09 | Kapa Biosystems | Modified DNA polymerases |
| EP2352818B1 (en) * | 2008-11-03 | 2016-02-10 | Kapa Biosystems, Inc. | Chimeric dna polymerases |
| GB201007384D0 (en) | 2010-04-30 | 2010-06-16 | Medical Res Council | Enzymes |
| WO2012154934A1 (en) * | 2011-05-11 | 2012-11-15 | New England Biolabs, Inc. | Dna polymerase variants with reduced exonuclease activity and uses thereof |
| US8921044B2 (en) | 2011-05-11 | 2014-12-30 | New England Biolabs, Inc. | DNA polymerase variants with reduced exonuclease activity and uses thereof |
| US8819970B2 (en) | 2011-08-04 | 2014-09-02 | Diana Foster | Multi-layered ornamental clothing |
| ES2674043T5 (es) | 2013-03-14 | 2021-11-03 | Illumina Inc | Polimerasas modificadas para una incorporación mejorada de análogos de nucleótidos |
| EP3161154B1 (en) | 2014-06-27 | 2020-04-15 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| US9677057B2 (en) | 2014-09-30 | 2017-06-13 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
-
2015
- 2015-06-25 EP EP15739706.8A patent/EP3161154B1/en active Active
- 2015-06-25 EP EP20158427.3A patent/EP3702471B1/en active Active
- 2015-06-25 ES ES20158427T patent/ES3060591T3/es active Active
- 2015-06-25 DK DK15739706.8T patent/DK3161154T3/da active
- 2015-06-25 WO PCT/US2015/037785 patent/WO2015200693A1/en not_active Ceased
- 2015-06-25 PL PL15739706T patent/PL3161154T3/pl unknown
- 2015-06-25 CN CN201580033818.6A patent/CN106536728A/zh active Pending
- 2015-06-25 ES ES15739706T patent/ES2788949T3/es active Active
- 2015-06-25 PT PT157397068T patent/PT3161154T/pt unknown
- 2015-06-25 AU AU2015279794A patent/AU2015279794B2/en active Active
- 2015-06-25 JP JP2016571250A patent/JP6737710B2/ja active Active
- 2015-06-25 US US14/750,814 patent/US9765309B2/en active Active
-
2017
- 2017-08-22 US US15/683,079 patent/US10870836B2/en active Active
-
2020
- 2020-04-28 JP JP2020079698A patent/JP2020127415A/ja active Pending
- 2020-11-20 US US16/953,882 patent/US12110514B2/en active Active
-
2021
- 2021-12-02 JP JP2021196287A patent/JP2022033882A/ja active Pending
- 2021-12-22 AU AU2021290289A patent/AU2021290289B2/en active Active
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