JP2017518750A5 - - Google Patents
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- Publication number
- JP2017518750A5 JP2017518750A5 JP2016571250A JP2016571250A JP2017518750A5 JP 2017518750 A5 JP2017518750 A5 JP 2017518750A5 JP 2016571250 A JP2016571250 A JP 2016571250A JP 2016571250 A JP2016571250 A JP 2016571250A JP 2017518750 A5 JP2017518750 A5 JP 2017518750A5
- Authority
- JP
- Japan
- Prior art keywords
- polymerase
- amino acid
- acid sequence
- functionally equivalent
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 125000003275 alpha amino acid group Chemical group 0.000 claims 25
- 230000035772 mutation Effects 0.000 claims 17
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 16
- 238000006467 substitution reaction Methods 0.000 claims 15
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 9
- 108020004511 Recombinant DNA Proteins 0.000 claims 6
- 102220532233 Paraneoplastic antigen Ma3_L408A_mutation Human genes 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 108020004634 Archaeal DNA Proteins 0.000 claims 1
- 241000203353 Methanococcus Species 0.000 claims 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 claims 1
- 102220477099 Protein C-ets-1_T514A_mutation Human genes 0.000 claims 1
- 241000205160 Pyrococcus Species 0.000 claims 1
- 241000205188 Thermococcus Species 0.000 claims 1
- 102220010377 rs104894722 Human genes 0.000 claims 1
- 102220127832 rs886044912 Human genes 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462018470P | 2014-06-27 | 2014-06-27 | |
| US62/018,470 | 2014-06-27 | ||
| PCT/US2015/037785 WO2015200693A1 (en) | 2014-06-27 | 2015-06-25 | Modified polymerases for improved incorporation of nucleotide analogues |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020079698A Division JP2020127415A (ja) | 2014-06-27 | 2020-04-28 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017518750A JP2017518750A (ja) | 2017-07-13 |
| JP2017518750A5 true JP2017518750A5 (enExample) | 2018-08-02 |
| JP6737710B2 JP6737710B2 (ja) | 2020-08-12 |
Family
ID=53716556
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016571250A Active JP6737710B2 (ja) | 2014-06-27 | 2015-06-25 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
| JP2020079698A Pending JP2020127415A (ja) | 2014-06-27 | 2020-04-28 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
| JP2021196287A Pending JP2022033882A (ja) | 2014-06-27 | 2021-12-02 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020079698A Pending JP2020127415A (ja) | 2014-06-27 | 2020-04-28 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
| JP2021196287A Pending JP2022033882A (ja) | 2014-06-27 | 2021-12-02 | ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US9765309B2 (enExample) |
| EP (2) | EP3702471B1 (enExample) |
| JP (3) | JP6737710B2 (enExample) |
| CN (1) | CN106536728B (enExample) |
| AU (2) | AU2015279794B2 (enExample) |
| DK (1) | DK3161154T3 (enExample) |
| ES (2) | ES2788949T3 (enExample) |
| PL (1) | PL3161154T3 (enExample) |
| PT (1) | PT3161154T (enExample) |
| WO (1) | WO2015200693A1 (enExample) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015200693A1 (en) | 2014-06-27 | 2015-12-30 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| US9677057B2 (en) | 2014-09-30 | 2017-06-13 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| TWI658139B (zh) * | 2016-11-01 | 2019-05-01 | 體學生物科技股份有限公司 | 用以提升核苷酸類似物併入之重組dna聚合酶 |
| EP3613851A4 (en) | 2017-01-09 | 2020-07-01 | MGI Tech Co., Ltd. | RECOMBINANT DNA POLYMERASE |
| WO2018148724A1 (en) * | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from pyrococcus furiosus |
| US20220127587A1 (en) | 2017-02-13 | 2022-04-28 | IsoPlexis Corporation | Polymerase enzyme from pyrococcus furiosus |
| EP3580351A1 (en) | 2017-02-13 | 2019-12-18 | Qiagen Sciences, LLC | Polymerase enzyme from 9°n |
| WO2018197945A1 (en) | 2017-04-23 | 2018-11-01 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
| EP3842545B1 (en) | 2017-04-23 | 2022-11-30 | Illumina, Inc. | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
| WO2018197950A1 (en) | 2017-04-23 | 2018-11-01 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
| CN108795900B (zh) * | 2017-04-27 | 2021-02-02 | 深圳华大智造科技股份有限公司 | Dna聚合酶及其制备方法 |
| WO2020047695A1 (zh) * | 2018-09-03 | 2020-03-12 | 深圳华大生命科学研究院 | 重组型kod聚合酶 |
| WO2020056044A1 (en) | 2018-09-11 | 2020-03-19 | Singular Genomics Systems, Inc. | Modified archaeal family b polymerases |
| JP7515413B2 (ja) | 2018-10-31 | 2024-07-12 | イラミーナ インコーポレーテッド | ポリメラーゼ、組成物、および使用方法 |
| EP3891275A2 (en) * | 2018-12-05 | 2021-10-13 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| US11293061B2 (en) * | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
| US11512295B2 (en) | 2019-09-12 | 2022-11-29 | Singular Genomics Systems, Inc. | Modified thermoccocus polymerases |
| JP2022549196A (ja) | 2019-09-23 | 2022-11-24 | ディーエヌエー スクリプト | ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加 |
| US11591629B2 (en) * | 2019-12-23 | 2023-02-28 | Cheng-Yao Chen | Method and kit for template-independent nucleic acid synthesis |
| US11034942B1 (en) | 2020-02-27 | 2021-06-15 | Singular Genomics Systems, Inc. | Modified pyrococcus polymerases and uses thereof |
| WO2021224677A1 (en) | 2020-05-05 | 2021-11-11 | Akershus Universitetssykehus Hf | Compositions and methods for characterizing bowel cancer |
| WO2022082482A1 (zh) * | 2020-10-21 | 2022-04-28 | 深圳华大生命科学研究院 | 重组型kod聚合酶 |
| JP2024522795A (ja) * | 2021-06-18 | 2024-06-21 | エレメント バイオサイエンシズ,インコーポレイティド | 操作されたポリメラーゼ |
| KR20240024924A (ko) | 2021-06-29 | 2024-02-26 | 애질런트 테크놀로지스, 인크. | 폴리머라제 돌연변이체 및 3'-oh 비차단 가역적 종결자와의 사용 |
| US12077789B2 (en) | 2021-08-14 | 2024-09-03 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| TWI869710B (zh) * | 2021-09-29 | 2025-01-11 | 呈堯 陳 | 用於無模板rna合成的核酸聚合酶變體、試劑套組及方法 |
| KR20250034991A (ko) | 2022-08-16 | 2025-03-11 | 알데브론 엘엘씨 | 합성 환형 핵산 생산을 위한 무세포 방법 |
| US20240141427A1 (en) | 2022-09-30 | 2024-05-02 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| US20260218140A1 (en) * | 2023-01-16 | 2026-07-30 | The Regents Of The University Of California | Engineered polymerase for threose nucleic acid synthesis |
| CN116286720B (zh) * | 2023-03-29 | 2026-07-17 | 赛纳生物科技(北京)有限公司 | 一种dna聚合酶 |
| WO2025260380A1 (zh) * | 2024-06-21 | 2025-12-26 | 华大工程生物学长荡湖研究所 | 重组型kod聚合酶 |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| US6228628B1 (en) * | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US5948666A (en) | 1997-08-06 | 1999-09-07 | Diversa Corporation | Isolation and identification of polymerases |
| US20050244870A1 (en) | 1999-04-20 | 2005-11-03 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
| US6492161B1 (en) | 1999-06-02 | 2002-12-10 | Prokaria Ltd. | Bacteriophage RM 378 of a thermophilic host organism |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US20030228616A1 (en) * | 1999-10-29 | 2003-12-11 | Stratagene | DNA polymerase mutants with reverse transcriptase activity |
| EP2100971A3 (en) | 2000-07-07 | 2009-11-25 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
| US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| AU2003259350A1 (en) | 2002-08-23 | 2004-03-11 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
| US20070048748A1 (en) * | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
| GB0509508D0 (en) * | 2005-05-10 | 2005-06-15 | Solexa Ltd | Improved polymerases |
| JP4990886B2 (ja) * | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | 改良ポリメラーゼ |
| US20070022459A1 (en) | 2005-07-20 | 2007-01-25 | Gaebel Thomas M Jr | Method and apparatus for boundary-based network operation |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| JP2009521227A (ja) | 2005-12-22 | 2009-06-04 | パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド | ヌクレオチドアナログ組込み用ポリメラーゼ |
| SG170802A1 (en) | 2006-03-31 | 2011-05-30 | Solexa Inc | Systems and devices for sequence by synthesis analysis |
| WO2008006781A2 (en) | 2006-07-14 | 2008-01-17 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
| EP2089517A4 (en) | 2006-10-23 | 2010-10-20 | Pacific Biosciences California | POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| EP2677309B9 (en) | 2006-12-14 | 2014-11-19 | Life Technologies Corporation | Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US20110045489A1 (en) * | 2008-04-22 | 2011-02-24 | New England Biolabs, Inc. | Polymerases for Incorporating Modified Nucleotides |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| WO2010062776A2 (en) * | 2008-11-03 | 2010-06-03 | Kapabiosystems | Chimeric dna polymerases |
| WO2010062779A2 (en) * | 2008-11-03 | 2010-06-03 | Kapabiosystems | Modified dna polymerases |
| GB201007384D0 (en) * | 2010-04-30 | 2010-06-16 | Medical Res Council | Enzymes |
| EP2707481B1 (en) | 2011-05-11 | 2016-07-13 | New England Biolabs, Inc. | Dna polymerase variants with reduced exonuclease activity and uses thereof |
| US8921044B2 (en) | 2011-05-11 | 2014-12-30 | New England Biolabs, Inc. | DNA polymerase variants with reduced exonuclease activity and uses thereof |
| US8819970B2 (en) | 2011-08-04 | 2014-09-02 | Diana Foster | Multi-layered ornamental clothing |
| WO2014142921A1 (en) | 2013-03-14 | 2014-09-18 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| WO2015200693A1 (en) | 2014-06-27 | 2015-12-30 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| US9677057B2 (en) | 2014-09-30 | 2017-06-13 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
-
2015
- 2015-06-25 WO PCT/US2015/037785 patent/WO2015200693A1/en not_active Ceased
- 2015-06-25 US US14/750,814 patent/US9765309B2/en active Active
- 2015-06-25 ES ES15739706T patent/ES2788949T3/es active Active
- 2015-06-25 EP EP20158427.3A patent/EP3702471B1/en active Active
- 2015-06-25 ES ES20158427T patent/ES3060591T3/es active Active
- 2015-06-25 CN CN201580033818.6A patent/CN106536728B/zh active Active
- 2015-06-25 EP EP15739706.8A patent/EP3161154B1/en active Active
- 2015-06-25 DK DK15739706.8T patent/DK3161154T3/da active
- 2015-06-25 PT PT157397068T patent/PT3161154T/pt unknown
- 2015-06-25 AU AU2015279794A patent/AU2015279794B2/en active Active
- 2015-06-25 PL PL15739706T patent/PL3161154T3/pl unknown
- 2015-06-25 JP JP2016571250A patent/JP6737710B2/ja active Active
-
2017
- 2017-08-22 US US15/683,079 patent/US10870836B2/en active Active
-
2020
- 2020-04-28 JP JP2020079698A patent/JP2020127415A/ja active Pending
- 2020-11-20 US US16/953,882 patent/US12110514B2/en active Active
-
2021
- 2021-12-02 JP JP2021196287A patent/JP2022033882A/ja active Pending
- 2021-12-22 AU AU2021290289A patent/AU2021290289B2/en active Active
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