ES2788949T3 - Polimerasas modificadas para la incorporación mejorada de análogos de nucleótidos - Google Patents

Polimerasas modificadas para la incorporación mejorada de análogos de nucleótidos Download PDF

Info

Publication number
ES2788949T3
ES2788949T3 ES15739706T ES15739706T ES2788949T3 ES 2788949 T3 ES2788949 T3 ES 2788949T3 ES 15739706 T ES15739706 T ES 15739706T ES 15739706 T ES15739706 T ES 15739706T ES 2788949 T3 ES2788949 T3 ES 2788949T3
Authority
ES
Spain
Prior art keywords
dna polymerase
polymerase
altered
amino acid
family
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15739706T
Other languages
English (en)
Spanish (es)
Inventor
Cheng-Yao Chen
Erin Bomati
Molly He
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Application granted granted Critical
Publication of ES2788949T3 publication Critical patent/ES2788949T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
ES15739706T 2014-06-27 2015-06-25 Polimerasas modificadas para la incorporación mejorada de análogos de nucleótidos Active ES2788949T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462018470P 2014-06-27 2014-06-27
PCT/US2015/037785 WO2015200693A1 (en) 2014-06-27 2015-06-25 Modified polymerases for improved incorporation of nucleotide analogues

Publications (1)

Publication Number Publication Date
ES2788949T3 true ES2788949T3 (es) 2020-10-23

Family

ID=53716556

Family Applications (2)

Application Number Title Priority Date Filing Date
ES15739706T Active ES2788949T3 (es) 2014-06-27 2015-06-25 Polimerasas modificadas para la incorporación mejorada de análogos de nucleótidos
ES20158427T Active ES3060591T3 (en) 2014-06-27 2015-06-25 Modified polymerases for improved incorporation of nucleotide analogues

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES20158427T Active ES3060591T3 (en) 2014-06-27 2015-06-25 Modified polymerases for improved incorporation of nucleotide analogues

Country Status (10)

Country Link
US (3) US9765309B2 (enExample)
EP (2) EP3702471B1 (enExample)
JP (3) JP6737710B2 (enExample)
CN (1) CN106536728B (enExample)
AU (2) AU2015279794B2 (enExample)
DK (1) DK3161154T3 (enExample)
ES (2) ES2788949T3 (enExample)
PL (1) PL3161154T3 (enExample)
PT (1) PT3161154T (enExample)
WO (1) WO2015200693A1 (enExample)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015200693A1 (en) 2014-06-27 2015-12-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
US9677057B2 (en) 2014-09-30 2017-06-13 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
TWI658139B (zh) * 2016-11-01 2019-05-01 體學生物科技股份有限公司 用以提升核苷酸類似物併入之重組dna聚合酶
EP3613851A4 (en) 2017-01-09 2020-07-01 MGI Tech Co., Ltd. RECOMBINANT DNA POLYMERASE
WO2018148724A1 (en) * 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
US20220127587A1 (en) 2017-02-13 2022-04-28 IsoPlexis Corporation Polymerase enzyme from pyrococcus furiosus
EP3580351A1 (en) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymerase enzyme from 9°n
WO2018197945A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3842545B1 (en) 2017-04-23 2022-11-30 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
WO2018197950A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN108795900B (zh) * 2017-04-27 2021-02-02 深圳华大智造科技股份有限公司 Dna聚合酶及其制备方法
WO2020047695A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
WO2020056044A1 (en) 2018-09-11 2020-03-19 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
JP7515413B2 (ja) 2018-10-31 2024-07-12 イラミーナ インコーポレーテッド ポリメラーゼ、組成物、および使用方法
EP3891275A2 (en) * 2018-12-05 2021-10-13 Illumina, Inc. Polymerases, compositions, and methods of use
US11293061B2 (en) * 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
JP2022549196A (ja) 2019-09-23 2022-11-24 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
WO2022082482A1 (zh) * 2020-10-21 2022-04-28 深圳华大生命科学研究院 重组型kod聚合酶
JP2024522795A (ja) * 2021-06-18 2024-06-21 エレメント バイオサイエンシズ，インコーポレイティド 操作されたポリメラーゼ
KR20240024924A (ko) 2021-06-29 2024-02-26 애질런트 테크놀로지스, 인크. 폴리머라제 돌연변이체 및 3'-oh 비차단 가역적 종결자와의 사용
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
TWI869710B (zh) * 2021-09-29 2025-01-11 呈堯 陳 用於無模板ｒｎａ合成的核酸聚合酶變體、試劑套組及方法
KR20250034991A (ko) 2022-08-16 2025-03-11 알데브론 엘엘씨 합성 환형 핵산 생산을 위한 무세포 방법
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
US20260218140A1 (en) * 2023-01-16 2026-07-30 The Regents Of The University Of California Engineered polymerase for threose nucleic acid synthesis
CN116286720B (zh) * 2023-03-29 2026-07-17 赛纳生物科技(北京)有限公司 一种dna聚合酶
WO2025260380A1 (zh) * 2024-06-21 2025-12-26 华大工程生物学长荡湖研究所 重组型kod聚合酶

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US5948666A (en) 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US20050244870A1 (en) 1999-04-20 2005-11-03 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
EP2100971A3 (en) 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
GB0509508D0 (en) * 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
JP4990886B2 (ja) * 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
US20070022459A1 (en) 2005-07-20 2007-01-25 Gaebel Thomas M Jr Method and apparatus for boundary-based network operation
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP2009521227A (ja) 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
WO2008006781A2 (en) 2006-07-14 2008-01-17 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20110045489A1 (en) * 2008-04-22 2011-02-24 New England Biolabs, Inc. Polymerases for Incorporating Modified Nucleotides
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010062776A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
WO2010062779A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Modified dna polymerases
GB201007384D0 (en) * 2010-04-30 2010-06-16 Medical Res Council Enzymes
EP2707481B1 (en) 2011-05-11 2016-07-13 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 (en) 2011-05-11 2014-12-30 New England Biolabs, Inc. DNA polymerase variants with reduced exonuclease activity and uses thereof
US8819970B2 (en) 2011-08-04 2014-09-02 Diana Foster Multi-layered ornamental clothing
WO2014142921A1 (en) 2013-03-14 2014-09-18 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
WO2015200693A1 (en) 2014-06-27 2015-12-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
US9677057B2 (en) 2014-09-30 2017-06-13 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues

Also Published As

Publication number Publication date
JP2020127415A (ja) 2020-08-27
PT3161154T (pt) 2020-06-08
ES3060591T3 (en) 2026-03-27
AU2021290289A1 (en) 2022-01-27
JP2022033882A (ja) 2022-03-02
US20210246435A1 (en) 2021-08-12
US12110514B2 (en) 2024-10-08
CN106536728B (zh) 2026-08-11
US20170355970A1 (en) 2017-12-14
US10870836B2 (en) 2020-12-22
AU2015279794A1 (en) 2016-12-22
HK1231517A1 (en) 2017-12-22
CA2951416A1 (en) 2015-12-30
CN106536728A (zh) 2017-03-22
AU2015279794B2 (en) 2021-09-30
EP3702471B1 (en) 2025-12-24
PL3161154T3 (pl) 2020-10-19
AU2021290289B2 (en) 2024-02-15
EP3702471C0 (en) 2025-12-24
DK3161154T3 (da) 2020-06-15
JP6737710B2 (ja) 2020-08-12
US20150376582A1 (en) 2015-12-31
EP3161154A1 (en) 2017-05-03
JP2017518750A (ja) 2017-07-13
US9765309B2 (en) 2017-09-19
EP3161154B1 (en) 2020-04-15
WO2015200693A1 (en) 2015-12-30
EP3702471A1 (en) 2020-09-02

Similar Documents

Publication Publication Date Title
AU2021290289B2 (en) Modified Polymerases For Improved Incorporation Of Nucleotide Analogues
US11198854B2 (en) Modified polymerases for improved incorporation of nucleotide analogues
US11781182B2 (en) Modified polymerases for improved incorporation of nucleotide analogues
ES2903045T3 (es) Mutantes de recombinasa
CA2951416C (en) Modified polymerases for improved incorporation of nucleotide analogues
HK40033009B (en) Modified polymerases for improved incorporation of nucleotide analogues
HK40033009A (en) Modified polymerases for improved incorporation of nucleotide analogues
HK1231517B (en) Modified polymerases for improved incorporation of nucleotide analogues
HK1235089B (en) Modified polymerases for improved incorporation of nucleotide analogues
HK1235089A1 (en) Modified polymerases for improved incorporation of nucleotide analogues