JP2017513525A5 - - Google Patents
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- Publication number
- JP2017513525A5 JP2017513525A5 JP2017506636A JP2017506636A JP2017513525A5 JP 2017513525 A5 JP2017513525 A5 JP 2017513525A5 JP 2017506636 A JP2017506636 A JP 2017506636A JP 2017506636 A JP2017506636 A JP 2017506636A JP 2017513525 A5 JP2017513525 A5 JP 2017513525A5
- Authority
- JP
- Japan
- Prior art keywords
- droplet
- nucleic acid
- droplets
- molecular species
- exposing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 30
- 108020004707 nucleic acids Proteins 0.000 claims 29
- 102000039446 nucleic acids Human genes 0.000 claims 29
- 238000000034 method Methods 0.000 claims 25
- 210000004027 cell Anatomy 0.000 claims 12
- 239000003814 drug Substances 0.000 claims 4
- 229940079593 drug Drugs 0.000 claims 4
- 239000012530 fluid Substances 0.000 claims 2
- 102000012410 DNA Ligases Human genes 0.000 claims 1
- 108010061982 DNA Ligases Proteins 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 201000011510 cancer Diseases 0.000 claims 1
- 231100000433 cytotoxic Toxicity 0.000 claims 1
- 230000001472 cytotoxic effect Effects 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 210000002865 immune cell Anatomy 0.000 claims 1
- 230000031700 light absorption Effects 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461981123P | 2014-04-17 | 2014-04-17 | |
| US61/981,123 | 2014-04-17 | ||
| PCT/US2015/026338 WO2015161177A1 (en) | 2014-04-17 | 2015-04-17 | Systems and methods for droplet tagging |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017513525A JP2017513525A (ja) | 2017-06-01 |
| JP2017513525A5 true JP2017513525A5 (enExample) | 2018-05-31 |
Family
ID=54324604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017506636A Pending JP2017513525A (ja) | 2014-04-17 | 2015-04-17 | 液滴タグ化のためのシステムおよび方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20170028377A1 (enExample) |
| EP (1) | EP3132072B1 (enExample) |
| JP (1) | JP2017513525A (enExample) |
| CN (1) | CN106661763A (enExample) |
| AU (1) | AU2015247416A1 (enExample) |
| CA (1) | CA2945794A1 (enExample) |
| WO (1) | WO2015161177A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LT3305918T (lt) | 2012-03-05 | 2020-09-25 | President And Fellows Of Harvard College | Būdai, skirti epigenetinių sekų sudarymui |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| CN107614096A (zh) | 2015-03-13 | 2018-01-19 | 哈佛学院院长及董事 | 使用扩增测定细胞 |
| WO2016168584A1 (en) | 2015-04-17 | 2016-10-20 | President And Fellows Of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| WO2018170515A1 (en) | 2017-03-17 | 2018-09-20 | The Broad Institute, Inc. | Methods for identifying and modulating co-occurant cellular phenotypes |
| US11072816B2 (en) | 2017-05-03 | 2021-07-27 | The Broad Institute, Inc. | Single-cell proteomic assay using aptamers |
| US12297426B2 (en) | 2019-10-01 | 2025-05-13 | The Broad Institute, Inc. | DNA damage response signature guided rational design of CRISPR-based systems and therapies |
| CA3157359A1 (en) | 2019-10-10 | 2021-04-15 | 1859, Inc. | Methods and systems for microfluidic screening |
| CN119654676A (zh) * | 2022-08-23 | 2025-03-18 | 徕卡显微系统Cms有限公司 | 连接物、标志物、捕获构建体和数据存储装置 |
| WO2024105001A1 (en) * | 2022-11-18 | 2024-05-23 | Leica Microsystems Cms Gmbh | Method for sequencing |
| WO2025054490A1 (en) * | 2023-09-08 | 2025-03-13 | Rasyn, Llc | Multi-omic analysis using beyond-poisson droplet loading |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2662135A3 (en) * | 2003-08-27 | 2013-12-25 | President and Fellows of Harvard College | Method for mixing droplets in a microchannel |
| US8592150B2 (en) * | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| CN101946010B (zh) * | 2007-12-21 | 2014-08-20 | 哈佛大学 | 用于核酸测序的系统和方法 |
| US8367370B2 (en) * | 2008-02-11 | 2013-02-05 | Wheeler Aaron R | Droplet-based cell culture and cell assays using digital microfluidics |
| US8808986B2 (en) * | 2008-08-27 | 2014-08-19 | Gen9, Inc. | Methods and devices for high fidelity polynucleotide synthesis |
| WO2012019765A1 (en) * | 2010-08-10 | 2012-02-16 | European Molecular Biology Laboratory (Embl) | Methods and systems for tracking samples and sample combinations |
| US9150852B2 (en) * | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| LT3305918T (lt) * | 2012-03-05 | 2020-09-25 | President And Fellows Of Harvard College | Būdai, skirti epigenetinių sekų sudarymui |
| CN111748607B (zh) * | 2012-08-14 | 2024-04-30 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
| WO2014047561A1 (en) * | 2012-09-21 | 2014-03-27 | The Broad Institute Inc. | Compositions and methods for labeling of agents |
-
2015
- 2015-04-17 WO PCT/US2015/026338 patent/WO2015161177A1/en not_active Ceased
- 2015-04-17 US US15/303,874 patent/US20170028377A1/en not_active Abandoned
- 2015-04-17 AU AU2015247416A patent/AU2015247416A1/en not_active Abandoned
- 2015-04-17 CN CN201580029304.3A patent/CN106661763A/zh active Pending
- 2015-04-17 CA CA2945794A patent/CA2945794A1/en not_active Abandoned
- 2015-04-17 EP EP15780044.2A patent/EP3132072B1/en active Active
- 2015-04-17 JP JP2017506636A patent/JP2017513525A/ja active Pending
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