JP2017513525A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2017513525A5 JP2017513525A5 JP2017506636A JP2017506636A JP2017513525A5 JP 2017513525 A5 JP2017513525 A5 JP 2017513525A5 JP 2017506636 A JP2017506636 A JP 2017506636A JP 2017506636 A JP2017506636 A JP 2017506636A JP 2017513525 A5 JP2017513525 A5 JP 2017513525A5
- Authority
- JP
- Japan
- Prior art keywords
- droplet
- nucleic acid
- droplets
- molecular species
- exposing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 30
- 108020004707 nucleic acids Proteins 0.000 claims 29
- 102000039446 nucleic acids Human genes 0.000 claims 29
- 238000000034 method Methods 0.000 claims 25
- 210000004027 cell Anatomy 0.000 claims 12
- 239000003814 drug Substances 0.000 claims 4
- 229940079593 drug Drugs 0.000 claims 4
- 239000012530 fluid Substances 0.000 claims 2
- 102000012410 DNA Ligases Human genes 0.000 claims 1
- 108010061982 DNA Ligases Proteins 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 201000011510 cancer Diseases 0.000 claims 1
- 231100000433 cytotoxic Toxicity 0.000 claims 1
- 230000001472 cytotoxic effect Effects 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 210000002865 immune cell Anatomy 0.000 claims 1
- 230000031700 light absorption Effects 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461981123P | 2014-04-17 | 2014-04-17 | |
| US61/981,123 | 2014-04-17 | ||
| PCT/US2015/026338 WO2015161177A1 (en) | 2014-04-17 | 2015-04-17 | Systems and methods for droplet tagging |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017513525A JP2017513525A (ja) | 2017-06-01 |
| JP2017513525A5 true JP2017513525A5 (enExample) | 2018-05-31 |
Family
ID=54324604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017506636A Pending JP2017513525A (ja) | 2014-04-17 | 2015-04-17 | 液滴タグ化のためのシステムおよび方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20170028377A1 (enExample) |
| EP (1) | EP3132072B1 (enExample) |
| JP (1) | JP2017513525A (enExample) |
| CN (1) | CN106661763A (enExample) |
| AU (1) | AU2015247416A1 (enExample) |
| CA (1) | CA2945794A1 (enExample) |
| WO (1) | WO2015161177A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3305918B1 (en) | 2012-03-05 | 2020-06-03 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| LT3134536T (lt) | 2014-04-21 | 2020-05-11 | President And Fellows Of Harvard College | Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui |
| WO2016149096A1 (en) | 2015-03-13 | 2016-09-22 | President And Fellows Of Harvard College | Determination of cells using amplification |
| EP3283629A4 (en) | 2015-04-17 | 2018-08-29 | President and Fellows of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| WO2017184707A1 (en) | 2016-04-19 | 2017-10-26 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| US12180546B2 (en) | 2017-03-17 | 2024-12-31 | Massachusetts Institute Of Technology | Methods for identifying and modulating co-occurant cellular phenotypes |
| US11072816B2 (en) | 2017-05-03 | 2021-07-27 | The Broad Institute, Inc. | Single-cell proteomic assay using aptamers |
| US12297426B2 (en) | 2019-10-01 | 2025-05-13 | The Broad Institute, Inc. | DNA damage response signature guided rational design of CRISPR-based systems and therapies |
| AU2020361681A1 (en) | 2019-10-10 | 2022-05-05 | 1859, Inc. | Methods and systems for microfluidic screening |
| CN119654676A (zh) * | 2022-08-23 | 2025-03-18 | 徕卡显微系统Cms有限公司 | 连接物、标志物、捕获构建体和数据存储装置 |
| WO2024105001A1 (en) * | 2022-11-18 | 2024-05-23 | Leica Microsystems Cms Gmbh | Method for sequencing |
| WO2025054490A1 (en) * | 2023-09-08 | 2025-03-13 | Rasyn, Llc | Multi-omic analysis using beyond-poisson droplet loading |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104069784B (zh) * | 2003-08-27 | 2017-01-11 | 哈佛大学 | 流体物种的电子控制 |
| US8592150B2 (en) * | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| EP2235210B1 (en) * | 2007-12-21 | 2015-03-25 | President and Fellows of Harvard College | Methods for nucleic acid sequencing |
| US8367370B2 (en) * | 2008-02-11 | 2013-02-05 | Wheeler Aaron R | Droplet-based cell culture and cell assays using digital microfluidics |
| US8808986B2 (en) * | 2008-08-27 | 2014-08-19 | Gen9, Inc. | Methods and devices for high fidelity polynucleotide synthesis |
| WO2012019765A1 (en) * | 2010-08-10 | 2012-02-16 | European Molecular Biology Laboratory (Embl) | Methods and systems for tracking samples and sample combinations |
| EP2675819B1 (en) * | 2011-02-18 | 2020-04-08 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| EP3305918B1 (en) * | 2012-03-05 | 2020-06-03 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| JP6367196B2 (ja) * | 2012-08-14 | 2018-08-01 | テンエックス・ジェノミクス・インコーポレイテッド | マイクロカプセル組成物および方法 |
| EP2898096B1 (en) * | 2012-09-21 | 2024-02-14 | The Broad Institute, Inc. | Methods for labeling of rnas |
-
2015
- 2015-04-17 CA CA2945794A patent/CA2945794A1/en not_active Abandoned
- 2015-04-17 AU AU2015247416A patent/AU2015247416A1/en not_active Abandoned
- 2015-04-17 US US15/303,874 patent/US20170028377A1/en not_active Abandoned
- 2015-04-17 JP JP2017506636A patent/JP2017513525A/ja active Pending
- 2015-04-17 EP EP15780044.2A patent/EP3132072B1/en active Active
- 2015-04-17 WO PCT/US2015/026338 patent/WO2015161177A1/en not_active Ceased
- 2015-04-17 CN CN201580029304.3A patent/CN106661763A/zh active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2992313T3 (es) | Captura de dianas en gotitas monodispersas | |
| JP2013255517A5 (enExample) | ||
| Grönroos et al. | Short‐term direct contact with soil and plant materials leads to an immediate increase in diversity of skin microbiota | |
| Hennink et al. | Novel crosslinking methods to design hydrogels | |
| JP2017515469A5 (enExample) | ||
| Mao et al. | Metal–organic frameworks in microfluidics enable fast encapsulation/extraction of DNA for automated and integrated data storage | |
| Hur et al. | Characteristics and clinical application of extracellular vesicle-derived DNA | |
| SA519410124B1 (ar) | تقنيات تشخيصية تقوم على نظام مؤثر crispr للكشف عن الفيروسات | |
| WO2016100976A3 (en) | Methods for identifying multiple epitopes in selected sub-populations of cells | |
| JP2018508198A5 (enExample) | ||
| Orellana et al. | Self-healing polymer nanocomposite materials by Joule effect | |
| BR112020015093A2 (pt) | Diagnóstico baseado no sistema de efetor crispr | |
| BR112015004864A2 (pt) | enriquecimento de classificação de células ativadas por fluorescência (facs) para gerar plantas | |
| AU2018329947A1 (en) | Multi-effector CRISPR based diagnostic systems | |
| WO2017011773A3 (en) | Codon-optimized nucleic acids encoding antibodies | |
| EA201990860A1 (ru) | Рнк-направляемые модифицирующие нуклеиновые кислоты ферменты и способы их применения | |
| HRP20211078T1 (hr) | Metoda za molekularno kodiranje nukleinskih kiselina pojedinačnih stanica | |
| WO2012069655A3 (en) | Improved capping modules for designed ankyrin repeat proteins | |
| BR112015004848A2 (pt) | plataforma de integração de transgene engenheirada (etip) para direcionamento de gene e empilhamento de característica | |
| HK1243333A1 (zh) | 用於修饰的t细胞的方法和组合物 | |
| ES2690386T3 (es) | Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína Cas o la propia proteína Cas, y sus usos | |
| EP3736337B1 (en) | Method of optically encoding discrete volumes using optically-encoded particles | |
| Natali et al. | The exosome-like vesicles of Giardia assemblages A, B, and E are involved in the delivering of distinct small RNA from parasite to parasite | |
| WO2011053845A3 (en) | Microvessels, microparticles, and methods of manufacturing and using the same | |
| Di et al. | Design, bioanalytical, and biomedical applications of aptamer-based hydrogels |