JP2017512306A - マイクロ流体チップ上の蛍光検出アッセイ - Google Patents
マイクロ流体チップ上の蛍光検出アッセイ Download PDFInfo
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Abstract
Description
ここで、E(r)はベクトル電界を表し、kは伝搬波ベクトルである。関連する磁界H(r)についても、同じ式を記述可能である。したがって、ベクトル界(Ex、Ey、Ez、Hx、Hy、Hz)の各成分は、スカラーヘルムホルツ方程式を立証する必要があり、ψ(r)は、上記電磁界成分のいずれかである。
屈折率プロファイルは、以下の通りである。
βは、導波方向に沿った伝搬波数であり、dは、コア層の厚さである。
ここで、
φSは、位相シフトである。ここから、導波モードの電界の一部が空間的に、減衰定数
でそれぞれ基板及びクラッドに延入していることが分かる。これら2つの指数関数的に減衰するテールは、導波モードのエバネセントテールと称し、例えば界面近くに位置付けられたフルオロフォア等の吸収分子の励起に利用可能である。減衰定数は主に、相対屈折率及び導波路の非対称度の他、励起波長によって決まる。
2 リーダユニット
3 診断装置
4 コンピュータシステム
5 データリンク
20 マイクロ流体システム
22 注入パッド
24 サンプル作成部
25 被験物質溶液
26、26’ 検出部位
27 捕獲スポット
28 毛細管ポンプ
30 マイクロ流体チャネル
31、31’ チャネルの側壁
32 アッセイ領域
60 フォトニックシステム、フォトニック層
61 導波路スプリッタ
62、62’ 入力導波路
62a 入力導波路
63 出力導波路
64 励起光線、励起光
65 その他の励起光
66 検出領域の導波路構造
67 導波路束
68 蛍光発光
70 光学系
72 光源
74 結合要素
75 結合要素
76 基準信号検出要素
78 観測者
80 検出ユニット
90 光キャビティ
102 基板層
104、104’ コア層
105、105’ コア層の表面
106、106’ クラッド層
107 中央部
108 スーパーストレート層
110 被験物質溶液
112 固定捕捉抗体/捕捉分子
114 抗原/被験物質分子
116 フルオロフォア、蛍光マーカ
117 捕捉抗体−抗原錯体
118 抗原−蛍光マーカ錯体
119 捕捉抗体−抗原−蛍光マーカ錯体
Claims (12)
- 1つ以上の生理学的サンプル上で蛍光検出アッセイを実行するアッセイユニット(1)であって、
マイクロ流体システム(20)を備えたマイクロ流体チップであり、該マイクロ流体チップ上に配置された1つ以上のマイクロ流体チャネル(30)を介して生理学的サンプル又は被験物質溶液(110)を搬送することができるマイクロ流体チップと、
前記マイクロ流体チップ上に配置された2つ以上の方形導波路構造(62、66、67、63)を備えたフォトニックシステム(60)であり、近赤外、可視、又は近紫外域の励起光(64、65)を導波することができるフォトニックシステム(60)と
を備え、
前記1つ以上のマイクロ流体チャネル及び前記2つ以上の導波路構造が、検出部位(26)において互いに交差しており、特定のマイクロ流体チャネル及び特定の導波路構造が互いに交差するアッセイ領域(32)において、前記導波路構造(66、67)のコア(104)の1つ以上の側方表面(105、105’)が、前記導波路構造内を導かれた光のエバネセント場が前記マイクロ流体チャネルの内側容積部の特定部分と重なるように、前記マイクロ流体チャネルの内側容積部に対して少なくとも部分的に対向した、アッセイユニット(1)。 - 前記アッセイ領域(32)において、前記導波路コア(104)の前記表面(105、105’)に1つ以上の捕獲スポット(27)が配置されており、前記捕獲スポットが、前記導波路コアの前記表面(105、105’)に固定されたアッセイの捕捉分子(112)の被膜を含む、請求項1に記載のアッセイユニット。
- 前記アッセイ領域(32)において、前記導波路構造(66、67)の前記コア層(104)が基板層(102)上に配置され、前記基板層の反対側に位置する前記コア(104)の上側表面(105)のみが前記マイクロ流体チャネル(30)の内側容積部に対向するように、前記コア層がクラッド層(106)に埋め込まれている、請求項1又は2に記載のアッセイユニット。
- 前記アッセイ領域(32)において、前記導波路構造(66、67)の前記コア層(104)が基板層(102)上に配置され、前記基板層の反対側に位置する前記コア(104)の上側表面(105)と2つの側方表面(105’)との両者が前記マイクロ流体チャネル(30)の内側容積部に対向するように、前記コア層は少なくとも部分的にクラッド層(106)に埋め込まれていない、請求項1又は2に記載のアッセイユニット。
- 前記導波路構造(66)が、単一の導波路又は一束の導波路を含み、前記導波路が、単一モード導波路又は多モード導波路である、請求項1〜4のいずれか一項に記載のアッセイユニット。
- 1つ以上の前記導波路(62、66、67、63)に光線を取り込む1つ以上の結合要素(74)並びに/又は前記1つ以上の導波路(62、66、63)から光線を取り出す1つ以上の結合要素(75)を有する、請求項1〜5のいずれか一項に記載のアッセイユニット。
- 請求項1〜6のいずれか一項に記載のアッセイユニット(1)上で実行された免疫学的検定の結果を読み出すリーダユニット(2)であって、
アッセイユニットを解放可能に搭載するホルダと、
励起光線(64)を発生する1つ又は複数の光源(72)と、
前記ホルダに搭載されたアッセイユニットにより放出された蛍光発光(68)を測定することができる検出ユニット(80)と
を備える、リーダユニット(2)。 - 前記検出ユニット(80)が、前記ホルダに搭載されたアッセイユニット(1)の前記蛍光発光(68)の2次元デジタル画像を取得することができる、請求項7に記載のリーダユニット。
- 前記検出ユニット(80)が、電荷結合素子チップ等のデジタル画像検出チップを備える、請求項7又は8に記載のリーダユニット。
- 前記ホルダに搭載されたアッセイユニット(1)の結合要素(74)に向かって励起光線(64)を導く光学要素を備える、請求項7〜9のいずれか一項に記載のリーダユニット。
- 前記ホルダに搭載されたアッセイユニット(1)の導波路(63)から取り出された励起光(65)の振幅を測定する検出要素(76)を備える、請求項7〜10のいずれか一項に記載のリーダユニット。
- 蛍光検出免疫学的検定を実行する診断装置(3)であって、請求項1〜6のいずれか一項に記載の1つ又は複数のアッセイユニット(1、1’)と、請求項7〜11のいずれか一項に記載のリーダユニット(2)とを備える、診断装置(3)。
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EP14158334.4 | 2014-03-07 | ||
EP14158334.4A EP2916125A1 (en) | 2014-03-07 | 2014-03-07 | Fluorescence-detected assays on microfluidic chips |
PCT/EP2015/054643 WO2015132347A1 (en) | 2014-03-07 | 2015-03-05 | Fluorescence-detected assays on microfluidic chips |
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US20240053268A1 (en) | 2024-02-15 |
JP6659173B2 (ja) | 2020-03-04 |
CA2939386A1 (en) | 2015-09-11 |
EP3114461A1 (en) | 2017-01-11 |
EP2916125A1 (en) | 2015-09-09 |
US11808704B2 (en) | 2023-11-07 |
US20170016827A1 (en) | 2017-01-19 |
CA2939386C (en) | 2023-10-10 |
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