JP2017039666A - Composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition for enhancing the stability of astaxanthins that is known to have useful effects such as skin whitening and aging prevention, and to provide a stabilizer and a stabilizing method.SOLUTION: The present invention relates to the composition containing: an ingredient (A) astaxanthins; and an ingredient (B) Lamiaceae, Melissa, Lemon balm plants or an extract thereof; Further, the composition contains an ingredient (C) collagen. The present invention relates to the stabilizer of astaxanthins containing Lamiaceae, Melissa, Lemon balm plants or an extract thereof as an active ingredient; and the stabilization method.SELECTED DRAWING: None

Description

  The present invention relates to a composition comprising component (A) astaxanthins; component (B) Labiatae Lepidoptera lemon balm plant or an extract thereof. The present invention also relates to an astaxanthin degradation inhibitor and a degradation inhibition method using a Labiatae genus Lemon balm plant or an extract thereof.

Astaxanthins are known to have functions such as an antioxidant effect, an anti-inflammatory effect (see Patent Document 1 and Patent Document 2), an anti-skin aging effect (see Patent Document 3), and a whitening effect (Non-Patent Document 1). ing. For this reason, adding astaxanthins to foods, cosmetics, raw materials for pharmaceuticals, processed products thereof, and the like has been studied and implemented.
However, since astaxanthins are structurally unstable, it is difficult to contain them in a stable state by photolysis or component oxidation.
In order to stabilize astaxanthins, contrivances such as the combined use of astaxanthins and ascorbic acid have been made so far (see Patent Document 4).
Further, Patent Documents 5 to 7 can be cited regarding the stabilization of carotenoid pigments.
In Patent Document 5, a. An oil-soluble plant extract containing astaxanthins extracted from the petals of Adonis Paraestina belonging to the genus Ranunculaceae, b. A dihydric alcohol having 4 to 8 carbon atoms; c. Polyphenols, d. An external preparation for skin containing a chelating agent is disclosed.
Patent Document 6 discloses that one or more of organic acids having two or more carboxyl groups (excluding citric acid), glycine, or salts thereof contain a carotenoid pigment or a carotenoid pigment. A method for stabilizing a carotenoid pigment added to a product is disclosed.
Patent Document 7 discloses the combined use of iron ions, iron chelating agents, dipropylene glycol, and water-soluble antioxidants.

However, astaxanthins are unstable as described above, and it is difficult to suppress the effects of light and other components and it is difficult to stabilize astaxanthins, so the conventional stabilization techniques are not sufficient. It was. Furthermore, when used in external preparations for skin, cosmetics, etc., the color of astaxanthins blended in the preparation is visible to the user, so the discoloration change is a big problem in quality. Furthermore, if astaxanthins are decomposed and faded, the antioxidant effect, anti-inflammatory effect, and skin aging preventive effect originally expected for astaxanthins cannot be sufficiently exhibited in the preparation.
For these reasons, it has been desired to stably mix astaxanthins known to have many useful effects.

JP-A-2-49091 Japanese Patent Laid-Open No. 9-143063 Japanese Patent Application Laid-Open No. 5-15536 JP-A-1-215263 JP 2010-105988 A JP-A-6-264055 JP 2012-31067 A

Int. J. Mol. Sci. 2014 (15) 8293-8315

  The problem to be solved by the present invention is to provide a composition for enhancing the stability of astaxanthins known to have useful effects such as whitening and anti-aging, and a stabilizer and a stabilization method. is there.

  As a result of intensive studies in view of such circumstances, the present inventors have found that astaxanthins can be stabilized by using a Labiatae genus Lemon balm plant or an extract thereof, and to complete the present invention. It came. By combining the astaxanthins with the Labiatae genus Lemon balm plant or an extract thereof, it is possible to exert a remarkable effect on preventing the astaxanthins from fading due to light. And by stabilizing astaxanthin suitably, the effects such as anti-aging effect and whitening effect of astaxanthin contained in cosmetics and the like can be remarkably enhanced, and as a result, such an effect of astaxanthin is improved. It is also possible to provide a composition in which is stabilized.

The present invention provides a composition containing component (A) astaxanthins and component (B) Labiatae genus Lemon balm plant or an extract thereof.
The present invention provides a cosmetic, an external preparation for skin, or a quasi-drug containing the composition.
This invention provides use of the said composition for manufacture of cosmetics, a skin external preparation, a quasi-drug, a pharmaceutical, food-drinks, or feed.
The present invention provides a stabilizer for astaxanthins, which comprises a Labiatae genus Lemon balm plant or an extract thereof as an active ingredient.

  ADVANTAGE OF THE INVENTION According to this invention, the composition which improves the stability of astaxanthin known to have useful effects, such as whitening and anti-aging, a stabilizer, and a stabilization method can be provided.

The component (A) astaxanthin in the present technology is a kind of carotenoid, and unless otherwise specified, includes astaxanthin and astaxanthin derivatives such as astaxanthin esters. The astaxanthins can be selected from astaxanthin and astaxanthin derivatives.
In general, as an effect of astaxanthins, an antioxidant effect, an anti-inflammatory effect, an anti-skin aging effect, a whitening effect and the like are known. Astaxanthin is widely distributed in nature and is generally known to exist as an astaxanthin fatty acid ester, and also exist as an astaxanthin protein (oborbin, cluster cyanine) bound to a protein in crustaceans and the like. Usually, in the extraction of naturally-derived astaxanthin, the ratio of astaxanthin and an astaxanthin fatty acid ester is increased so that the above-described effects are suitably exhibited.

Furthermore, astaxanthin derivatives include astaxanthin salts, oxidized forms, reduced forms, geometrical isomers, optical isomers, glycosides, ester forms, and the like, as appropriate, in addition to naturally-derived astaxanthin esters.
More specific astaxanthin derivatives include, for example, amino acid esters such as glycine and alanine, carboxylic acid esters such as acetate ester and citrate ester and salts thereof; inorganic acid esters such as phosphate ester and sulfate ester and salts thereof; Glycosides such as glycosides; highly unsaturated fatty acids such as eicosapentaenoic acid and docosahexaenoic acid; unsaturated fatty acids such as oleic acid and linoleic acid; fatty acid esters selected from saturated fatty acids such as palmitic acid and stearic acid Examples thereof include monoesters and the same or different diesters. Examples of the fatty acid include lower fatty acids having 2 to 4 carbon atoms, intermediate fatty acids having 5 to 12 carbon atoms, and higher fatty acids having 13 or more carbon atoms (for example, 16 to 22 carbon atoms).

Astaxanthin in the narrow sense is a red pigment having an absorption maximum at 476 nm (ethanol) and 468 nm (hexane), and its chemical structure is 3,3'-dihydroxy-β, β-carotene-4,4'-dione (C ₄₀ H ₅₂ 0 ₄ , molecular weight 596.82). This astaxanthin has 3S, 3S'-form, 3S, 3R'-form (meso-form), 3R, 3R'-form by the configuration of the 3 (3 ')-position hydroxyl groups in the ring structure at both ends of the molecule. There are three isomers of the body. There are also cis- and trans- isomers of conjugated double bonds at the center of the molecule. For example, all cis-, 9-cis and 13-cis isomers. Any one can be used in the present technology.

  Here, the hydroxyl group at the 3,3′-position can form an ester with a fatty acid. Astaxanthin obtained from krill is a diester form in which two fatty acids are bound, and those obtained from hematococcus algae (H. pluvialis) are 3S, 3S'- forms, which contain many monoester forms in which one fatty acid is bound. . Astaxanthin obtained from red yeast (Phaffia Rhodozyma) has a structure opposite to that of 3S, 3S'-form, which is usually found naturally in 3R, 3R'-form.

The component (A) astaxanthins of the present technology includes those extracted and purified from animals, plants, algae, bacteria and fungi. Moreover, it is not limited to the thing of natural origin, Any thing can be used if it is obtained according to a conventional method (organic synthetic product). It is also possible to use a mixture of these as appropriate. Naturally derived astaxanthin is derived from astaxanthin produced by a living organism.
Astaxanthins used in the present technology may be, for example, those extracted from natural products such as krill, salmon, trout, cypress, red yeast, Haematococcus algae, or synthetic products. In addition, for example, Katakura Chikkarin Co., Ltd., Marine Daio Co., Ltd., Swiss Pharmaceuticals, Takeda Shiki Co., Ltd., Fuji Chemical Industry Co., Ltd., Oriza Yuka Co., Ltd., Wako Pure Chemical Industries, Ltd., Biogenic General products commercially available from companies and the like can be used.
Among these, those extracted from Haematococcus alga (hereinafter also referred to as Haematococcus alga extract) are particularly preferable from the viewpoint of quality and productivity.
The extraction solvent for obtaining astaxanthins from natural products may be an aqueous solvent or an organic solvent.
As the organic solvent, alcohol (methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, etc.), ethyl acetate, ether, hexane, or the like can be used. Also, carbon dioxide in a supercritical state can be used. These solvents may be used alone or in combination of two or more.

  Specific examples of haematococcus algae extracts that can be used in the present technology include Haematococcus pluvialis, Haematococcus lacustris, Haematococcus capensis, and Haematococcus capensis Examples thereof include Haematococcus droebakensis and Haematococcus zimbabwiensis. Any commercially available Haematococcus alga extract can be used.

What is necessary is just to mix | blend using the product (for example, biological product) containing astaxanthin, when astaxanthins are included in the composition of this technique. At this time, the content of astaxanthins in the product used as the component (A) (astaxanthin-free body equivalent) is not particularly limited, but is preferably 0.01 to 50% by mass (hereinafter simply referred to as “%”). It is more preferably 0.1 to 30%, and particularly preferably 1 to 20%.
Moreover, the amount of astaxanthins in the composition of the present technology is preferably 0.000001 to 5%, more preferably 0.0001 to 0.5%, and still more preferably 0.0001 to 0.1%. %.
The dose or coating amount of component (A) astaxanthin is usually 0.001 to 10 mg (astaxanthin-free body equivalent), preferably 0.01 to 1 mg, or taken once or three times a day. It may be applied.

Component (B) in this technology (Lamiaceae) Melissa Lemon balm plant (hereinafter also referred to as "Lemon balm plant"), the scientific name is called (Melissa officinalis), also known as Melissa, It is also called “Yamahakka”. The lemon balm plant is widely used for flavoring food and drink.
In addition, the use site of the lemon balm plant is not particularly limited to roots, rhizomes, stems, leaves, flowers, fruits, seeds, etc., but it is preferable to use the above-ground part in terms of stabilizing astaxanthins, and leaves are more preferable. The plant is preferably used as it is or after being pulverized. Such a plant can be used as it is or as a squeezed juice obtained by squeezing it, a dried product obtained by drying the plant itself or a pulverized product thereof, or an extract extracted therefrom. Is preferred.

The extraction method from the lemon balm plant is not particularly limited, and a general extraction method can be used.
The lemon balm extract is cut at any one or more of whole grass, leaves, petals, seeds, roots, rhizomes, etc. without drying or drying, then at low temperature (for example, less than 4 ° C.), normal temperature (for example, 4 -40 ° C) and heating (for example, 40-100 ° C), and it is obtained by extraction with a solvent. In addition, it is desirable to use 50-10000 mL of solvent with respect to 100 g of the lemon balm plants.

Moreover, as a solvent for extraction, water, an organic solvent, etc. are mentioned, for example.
Examples of the organic solvent include alcohols (monovalent, divalent or trivalent or higher alcohols), esters (ethyl acetate, butyl acetate, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether). Etc.), hydrocarbons (hexane, benzene, pentane, etc.) and the like. Also, carbon dioxide in a supercritical state can be used.
As said monohydric alcohol, methanol, ethanol, isopropanol etc. are mentioned, for example. Examples of the dihydric alcohol include 1,3-butylene glycol, ethylene glycol, propylene glycol, and the like. Examples of the trihydric alcohol include glycerin. Examples of the trihydric or higher alcohol include sugar alcohols such as sorbitol and maltitol, polyethylene glycol, and the like. Among these, C1-C4 (preferably 2-3) lower alcohol is suitable.
From the viewpoint of use, water and / or a water-soluble organic solvent are preferable, and water, lower alcohol (more preferably, ethanol, methanol, 1,3-butylene glycol, etc.) or a mixed solvent thereof is preferable. .
From the illustrations of these extraction solvents, they may be used alone or in combination of two or more. Moreover, you may mix and use what was extracted twice or more using a different solvent.

  As an example of a preferable extraction method, the extraction is performed for 1 to 5 days using a lower alcohol (ethanol or 1,3-butylene glycol) having a content solvent concentration of 0 to 100% by volume. In addition, the density | concentration of a content machine solvent becomes like this. Preferably it is 30 volume% or more, More preferably, it is 50-100 volume%. The temperature at the time of extraction is not particularly limited, but for example, room temperature extraction (for example, extraction at 0 to 40 ° C.) or heat extraction at 30 to 70 ° C. is preferable.

The extract of the lemon balm plant by the solvent may be in any form such as liquid, paste, gel, solid, and powder. The extract can be used as it is after preparation, but it may be used after being concentrated, dried or spray-dried and dissolved again in water or a polar solvent.
Furthermore, if necessary, it can be used after being subjected to treatments such as decolorization, deodorization, and desalting using filtration, activated carbon, ion exchange resin and the like within a range not affecting the effects of the present invention. Moreover, it can also fractionate and use by methods, such as ultrafiltration, ion-exchange chromatography, and gel filtration chromatography.

  The content (dry solid content) of the component (B) lemon balm plant or extract thereof of the present technology in the composition of the present technology is preferably 0.00001 to 1.0%, more preferably 0.0001 to 0.1%. And particularly preferably 0.001 to 0.01%.

  The content ratio (dry solid content) in the composition of the present technology between the component (A) astaxanthins and the component (B) lemon balm plant or an extract thereof is not particularly limited, but for the stabilization effect of the astaxanthins ( The mass ratio of A) / (B) is preferably 0.001 to 100, more preferably 0.01 to 10, and particularly preferably 0.02 to 3.

Furthermore, if the present invention contains collagen as component (C), the photostability of astaxanthins can be further enhanced. The origin of these collagens may be derived from marine organisms such as fish and jellyfish, may be derived from terrestrial organisms such as cattle, pigs, and horses, or may be other than that. In addition, collagen may be used as it is, or may be used after being subjected to a treatment such as atelolation, if necessary, and may be used after being hydrolyzed by acid, alkali, enzyme treatment or the like. it can. Among these, from the viewpoint of further improving the stability, collagen, a hydrolyzate of collagen, and acylated atelocollagen are preferable; and water-soluble collagen, hydrolyzed collagen, atelocollagen, and succinyl atelocollagen are particularly preferable.

Examples of collagen to be blended in the composition of the present invention or the stabilizer for astaxanthin include, for example, Marinegen S-03 (PF) (manufactured by Nitta Gelatin Co., Ltd.), Marinegen peptide MP (PF) (manufactured by Nitta Gelatin Co., Ltd.). Commercially available products such as 1% PE of shark fin telocollagen (manufactured by Koken Co., Ltd.), 1% PE of porcine atelocollagen (manufactured by Koken Co., Ltd.), ocean collagen (manufactured by Airwater Co., Ltd.), and succinyl ocean collagen (manufactured by Airwater Co., Ltd.) You may use and what was extracted in accordance with the conventional method may be used.

About content of a component (C), what is necessary is just to set suitably as needed, For example, 0.000001-3 weight%, Preferably it is 0.000001-1 weight%, More preferably, it is 0.00001-0.1. % By weight, more preferably 0.00003 to 0.015% by weight, particularly preferably 0.00015 to 0.015% by weight.

  In the composition of the present technology, components that are usually used in preparations such as cosmetics, quasi-drugs, and pharmaceuticals can be added to the composition of the present technology, as long as the effects of the present invention are not impaired. Examples of the component include water (purified water, hot spring water, deep water, etc.), oil agent, surfactant, metal soap, gelling agent, powder, alcohols, water-soluble polymer, film forming agent, resin, ultraviolet ray Protective agent, inclusion compound, antibacterial agent, fragrance, deodorant, salt, pH adjuster, refreshing agent, animal / microbe-derived extract, plant extract, blood circulation promoter, astringent, antiseborrheic agent, whitening agent , Anti-inflammatory agents, active oxygen scavengers, cell activators, moisturizers, chelating agents, keratolytic agents, enzymes, hormones, vitamins and the like.

The composition of the present technology can be obtained by a known production method. As a use of the composition of the present technology, it can be used for cosmetics, pharmaceuticals, foods and drinks, functional foods, external preparations for skin, quasi drugs, feeds and the like. The compositions of the present technology can be used to make them.
Moreover, as a form which mix | blends the composition of this technique, cosmetics, a quasi-drug, a pharmaceutical, food-drinks, functional foods (for example, supplement, food for specific health), animal feed, etc. are mentioned, for example. It is done. When the composition of the present technology is blended in these forms, an appropriately acceptable component may be contained.
The present technology can be suitably used particularly for cosmetics and quasi drugs, and in this case, the component (A) and the component (B) are contacted or applied to the skin. Specific examples of cosmetics and quasi-drugs include, for example, basic cosmetics such as emulsions, creams, skin lotions, beauty liquids, packs, and cleaning agents, makeup cosmetics such as foundations, blushers, and lipsticks, and hair nourishing agents. , Hair tonics, shampoos, rinses and other hair cosmetics, dispersions, ointments, solutions, tablets, aerosols, patches, poultices, liniments, and the like.

Conventionally, astaxanthin has a useful effect as described above, but it is difficult to obtain the expected effect because of its structural instability, and it is difficult to maintain the effect over a long period of time.
However, as shown in Examples below, the astaxanthin in the composition can be stabilized by blending the lemon balm plant or the extract thereof into the composition containing the astaxanthin.

By using the component (B) of the present technology, the lemon balm plant or an extract thereof, it becomes possible to improve the stability of astaxanthins, and thereby the effectiveness of astaxanthins can be effectively exhibited. For example, the lemon balm plant or an extract thereof can exert a remarkable effect in preventing discoloration of astaxanthins and suppressing photolysis.
Astaxanthins are useful for preventing and improving rough skin and wrinkles, preventing and improving spots, and further useful for preventing aging and whitening. Therefore, the lemon balm plant or its extract has these effects of astaxanthins. (For example, an anti-aging effect and a whitening effect) can be dramatically improved. By using the lemon balm plant or an extract thereof, the effect of astaxanthins can be maintained for a longer period of time, or even if the content of astaxanthins is reduced, the same or higher effect can be expected.

Therefore, the composition containing the component (A) astaxanthins of the present technology and the component (B) Labiatae genus Lemon balm plant or an extract thereof is excellently stable in the efficacy of the excellent astaxanthins as described above. Can be demonstrated.
Moreover, since the lemon balm plant of the present technology or an extract thereof has a stabilizing action of astaxanthins, it can be used as an astaxanthin stabilizer that contains the lemon balm plant or an extract thereof as an active ingredient. It is.
The lemon balm plant of the present technology or an extract thereof may be used for stabilization of astaxanthins, and can be used for various preparations intended for stabilization of astaxanthins. It can also be used for manufacturing.
Therefore, the lemon balm plant of the present technology or an extract thereof is used for the stabilization of astaxanthins, such as an external preparation for skin, a cosmetic, a pharmaceutical product, a quasi-drug, a food or drink, or a functional food (for example, food for specified health use, supplement) Etc.).
Although the usage-amount (dry solid content) of the said lemon balm plant of this technique or its extract is not specifically limited, Preferably it is 0.01-1000 mass parts with respect to 1 mass part of astaxanthins, More preferably, it is 0.1. -100 mass parts.

  EXAMPLES Hereinafter, although an Example etc. are given and this invention is demonstrated further more concretely, the scope of the present invention is not limited to these.

[Production Example 1: Production of Lemon Balm Hot Water Extract 1]
Lemon balm (scientific name: Melissa officinalis) 100 g was chopped, added with 3000 mL of water, extracted at 90 ° C. for 6 hours, filtered to remove insoluble matters, and lemon balm extract 1 was obtained. The dry solid content of this extract was 2.0%.

[Production Example 2: Production of Lemon Balm 50% Ethanol Extract 2]
Lemon balm (scientific name Melissa officinalis) 100 g of leaves, add 3000 mL of 50 volume% ethanol aqueous solution, extract in a cool dark place (4-10 ° C.) for 2 days, filter to remove insoluble matter, lemon balm 50% ethanol extract 2 was obtained. The dry solid content of this extract was 2.0%.

[Production Example 3: Production of Lemon Balm 90% Ethanol Extract 3]
Chop 100 g of lemon balm (scientific name Melissa officinalis) leaves, add 3000 mL of 90 vol% ethanol aqueous solution, extract at about 55 ° C for 24 hours, leave at 5 ° C for 1 week, filter off insoluble matter. Lemon balm 90% ethanol extract 3 was obtained. The dry solid content of this extract was 1.5%.

Example 1
<Astaxanthin Photolysis Inhibition Effect (Stabilization Effect) Test 1>
Astaxanthin (manufactured by Wako Pure Chemical Industries, Ltd.) 0.00025% by mass, tri (capric acid / caprylic acid) glyceryl (manufactured by Nisshin Eulio) 0.00475% by mass, dimethyl sulfoxide 25% by mass, lemon balm of Production Example 3 An aqueous solution containing the extract as a dry solid content at a concentration of 0.00008 to 0.01% by mass was prepared. 200 μL of each of these aqueous solutions was added to each 96-well plate, and the absorbance was measured. Thereafter, each solution was irradiated with ultraviolet rays (UVA + B) for 5 minutes × 3 times, and the absorbance was measured after irradiation. Moreover, the solution which does not add a lemon balm plant extract was made into control (0%). In addition, astaxanthin content in [Example] is astaxanthin-free body conversion.

Astaxanthin residual rate was measured by measuring the absorbance of each sample at 470 nm,
Astaxanthin residual rate (%) = ((A−B) / (C−D)) × 100 (Formula 1)
A = Absorbance of UV-irradiated astaxanthin-containing system B = Absorbance of UV-irradiated astaxanthin-free system C = Absorbance of UV-unirradiated astaxanthin-containing system D = Absorbance of non-UV-irradiated astaxanthin-containing system

  The results are shown in Table 1. From this result, the lemon balm plant extract was able to suitably prevent discoloration of astaxanthins under ultraviolet irradiation conditions. Astaxanthins are decomposed and faded by ultraviolet rays, and the effect is reduced. This ultraviolet ray has a more chemical effect than sunlight. Therefore, the lemon balm plant extract had an excellent effect on the photodegradation inhibition of astaxanthins. Therefore, the lemon balm plant extract can increase the stability of astaxanthins and maintain the effect over a long period of time.

Example 2
<Astaxanthin Photolysis Inhibition Effect (Stabilization Effect) Test 2>
Astaxanthin (manufactured by Wako Pure Chemical Industries, Ltd.) 0.00025% by mass, tri (capric acid / caprylic acid) glyceryl (manufactured by Nisshin Eulio) 0.00475% by mass, dimethyl sulfoxide 25% by mass, lemon balm of Production Example 3 An aqueous solution containing 0.01% by mass of the extract as a dry solid content or 0.02% by mass of fukahirea telocollagen (manufactured by Koken Co., Ltd.) or both was prepared. 200 μL of each of these aqueous solutions was added to each 96-well plate, and the absorbance was measured. Thereafter, each solution was irradiated with ultraviolet rays (UVA + B) for 5 minutes × 3 times, and the absorbance was measured after irradiation. Moreover, the solution which does not add a lemon balm plant extract was made into control (0%). In addition, astaxanthin content in [Example] is astaxanthin-free body conversion.

Astaxanthin residual rate was measured by measuring the absorbance of each sample at 470 nm,
Astaxanthin residual rate (%) = ((A−B) / (C−D)) × 100 (Formula 1)
A = Absorbance of UV-irradiated astaxanthin-containing system B = Absorbance of UV-irradiated astaxanthin-free system C = Absorbance of UV-unirradiated astaxanthin-containing system D = Absorbance of non-UV-irradiated astaxanthin-containing system

The results are shown in Table 2. From this result, it was shown that the lemon balm plant extract suitably prevented the degradation and discoloration of astaxanthins by ultraviolet rays. Moreover, it turned out that the discoloration inhibitory effect of astaxanthin increases further by combining collagen with a lemon balm plant extract from the result of Table 2. Therefore, by combining lemon balm plant extract, or lemon balm plant extract and collagen, an excellent effect on the photodegradation suppression of astaxanthins can be exhibited, and the stability of astaxanthins can be increased and the effect maintained for a long time. Is possible.
As a comparative example, ascorbic acid 0.01 mM, which is a commercially available antioxidant, was tested in the same manner. Astaxanthin residual rate was about 44%. Lemon balm plant extract 0.01% was 0% ascorbic acid. It can be said that it has an astaxanthin stabilizing effect superior to 0.01 mM.

Example 3 [Formulation in lotion]
(Ingredient) (%)
1 Glycerin 5.0
2 1,3-butylene glycol 5.0
3 Lactic acid 0.05
4 Sodium lactate 0.1
5 Water-soluble collagen (Note 1) 0.01
6 Lemon balm extract 1 0.05
7 Polyoxyethylene (20E.O.) sorbitan monooleate 1.2
8 Ethanol 8.0
9 Oil containing 5% astaxanthin (Note 2) 0.001
10 Methyl paraoxybenzoate 0.1
11 Fragrance 0.05
12 Remaining amount of purified water
(Note 1) PANCOGEN MARINE (manufactured by GATTEFOSSE)
(Note 2) Astaxanthin-5C (manufactured by Oriza Oil Chemical Co., Ltd.)

(Production method)
A: Components (7) to (11) are mixed and dissolved.
B: Components (1) to (6) and (12) are mixed and dissolved.
C: A was added to B and mixed to obtain a skin lotion.

Example 4 [Formulation in emulsion]
(Ingredient) (%)
1 Polyoxyethylene monostearate (20E.O.) sorbitan 1.0
2 Polyoxyethylene trioleate (20E.O.) sorbitan 0.5
3 Glyceryl monostearate 1.0
4 Stearic acid 0.5
5 Behenyl alcohol 0.5
6 Squalane 8.0
7 Carboxyvinyl polymer 0.1
8 Methyl paraoxybenzoate 0.1
9 Sodium hydroxide 0.05
10 Purified water remaining 11 Ethanol 5.0
12 Lemon balm extract 2 0.01
13 Oil containing 1% astaxanthin (Note 3) 0.01
14 Fragrance 0.05
(Note 3) Astaxanthin derived from Adonis Palestine flowers

(Production method)
A: Components (1) to (6) are uniformly mixed at 70 ° C.
B: Components (7) to (10) are uniformly mixed at 70 ° C. C: A is added to B, emulsified, and cooled to room temperature.
D: (11) to (14) were added to C and mixed uniformly to obtain an emulsion.

Example 5 [Formulation in liquid foundation (oil-in-water cream)]
(Ingredient) (%)
1 1,3 Butylene glycol 5.0
2 Hydrogenated soybean phospholipid 0.5
3 Titanium oxide 5.0
4 Bengala 0.1
5 Yellow iron oxide 1.0
6 Black iron oxide 0.05
7 Acrylic acid / alkyl methacrylate copolymer (Note 4) 0.5
8 Triethanolamine 1.5
9 Purified water remaining 10 Glycerin 5.0
11 Ethyl paraoxybenzoate 0.1
12 Stearic acid 0.9
13 Glycerol monostearate 0.3
14 cetostearyl alcohol 0.4
15 Polyoxyethylene (20E.O.) sorbitan monooleate 0.2
16 Polyoxyethylene trioleate (20E.O.) sorbitan 0.2
17 2-Ethylhexyl paramethoxycinnamate 5.0
18 Lemon balm extract 3 0.05
19 Oil containing 5% astaxanthin (Note 5) 0.05
20 Perfume 0.02
(Note 4) Pemulen TR-2 (manufactured by NOVEON)
(Note 5) Astaxanthin derived from Haematococcus spurbiaris

(Production method)
A: Disperse components (1) to (6).
B: Components (7) to (11) are added to A and mixed uniformly at 70 ° C.
C: Components (12) to (17) are uniformly mixed at 70 ° C.
D: B is added to C to emulsify, and cooled to room temperature.
E: Components (18) to (20) were added to D to obtain an oil-in-water cream liquid foundation.

Example 6 [Formulation in sunscreen cosmetic (water-in-oil cream)]
(Ingredient) (%)
1 Polyoxyethylene (20E.O.) sorbitan monolaurate 0.2
2 Polyoxyethylene (60) hydrogenated castor oil 0.1
3 Remaining purified water 4 Dipropylene glycol 10.0
5 Magnesium sulfate 0.5
6 Ascorbic acid glucoside 2.0
7 Lemon balm extract 1 0.3
8 Hydrolyzed collagen (Note 6) 0.02
9 PEG-9 polydimethylsiloxyethyl dimethicone 3.0
10 Decamethylcyclopentasiloxane 20.0
11 Isotridecyl isononanoate 5.0
12 2-Ethylhexyl paramethoxycinnamate 8.0
13 Oil containing 5% astaxanthin (Note 7) 0.01
14 Dimethylstearyl ammonium hectorite 1.2
(Note 6) Marine Gen Peptide MP (PF) (Nitta Gelatin Co., Ltd.)
(Note 7) ASTAX-S (manufactured by Marine Daiosha)

(Production method)
A: Components (1) to (8) are uniformly dispersed.
B: Components (9) to (14) are uniformly dispersed.
C: While stirring B, A was gradually added to emulsify to obtain a water-in-oil cream sunscreen cosmetic.

Example 7 [Formulation in ointment]
(Ingredient) (%)
1 Triethanolamine 2.0
2 Glycerin 5.0
3 Dipotassium glycyrrhizinate 0.5
4 Remaining amount of purified water 5 Stearic acid 18.0
6 Cetanol 4.0
7 Lemon balm extract 2 0.05
8 Oil containing 20% astaxanthin (Note 8) 0.01
9 dl-α-tocopherol acetate 0.2
10 Methyl paraoxybenzoate 0.1
(Note 8) Astaxanthin-20C (manufactured by Oriza Oil Chemical Co., Ltd.)

(Production method)
A: Components (1) to (4) are uniformly dissolved and kept at 75 ° C.
B: Components (5) to (10) are heated and mixed and kept at 75 ° C.
C: B was gradually added to A to obtain an ointment.

Example 8 [Formulation in lotion preparation]
(Ingredient) (%)
1 Polyoxyethylene (20E.O.) sorbitan monolaurate
1.2
2 Ethanol 8.0
3 Oil containing 5% astaxanthin (Note 9) 0.001
4 Methyl paraoxybenzoate 0.2
5 Glycerin 5.0
6 1,3-butylene glycol 6.5
7 Lemon balm extract 1 0.03
8 Succinyl atelocollagen (Note 10) 0.05
9 Purified water Residual amount (Note 9) Astaxanthin derived from Fukujugusa (Note 10) Succinyl ocean collagen (manufactured by Air Water Corporation)

(Production method)
A: Components (1) to (4) are mixed and dissolved.
B: Components (5) to (9) are mixed and dissolved.
C: A and B were mixed to make a lotion agent.

Example 9 [Formulation in lip balm]
(Ingredient) (%)
1 Candelilla wax 4.0
2 Ethylene / propylene copolymer 10.0
3 Pentaerythritol rosinate 5.0
4 Cetyl 2-ethylhexanoate 22.0
5 Vaseline 10.0
6 Acetic acid liquid lanolin 15.0
7 Diisostearyl malate 25.0
8 Diglyceryl triisostearate 9 Vitamin E 0.5
10 1,3-butylene glycol 1.0
11 Smoke-like silicic acid 1.0
12 Lemon balm extract 3 0.05
13 Oil containing 5% astaxanthin (Note 11) 0.3
13 Phenoxyethanol 0.3
14 Dibutylhydroxytoluene 0.1
(Note 11) Krill-derived astaxanthin

(Production method)
A: Components (1) to (9) are uniformly dissolved and mixed at 100 ° C.
B: (10), (11) and (12) are added and mixed uniformly.
C: B, (13) and (14) are added to A and mixed and dispersed.
D: After defoaming C, the container was filled and cooled to obtain a lip balm.

The lotions, emulsions, oil-in-water cream liquid foundations, water-in-oil cream sunscreen cosmetics, ointments, lotions and lip balms described in Examples 3 to 9 all have the effect of inhibiting photodegradation of astaxanthin. It was excellent and stable, and was a formulation useful for exerting skin effects such as improvement of skin roughness, prevention of aging and prevention / improvement of spots.

Composition comprising astaxanthin of the present technology and Labiatae genus lemon balm plant or extract thereof, and stabilizer and stabilization method for astaxanthins using the lemon balm plant or extract thereof It is possible to improve the efficacy of astaxanthins, and for example, it is useful for exerting skin effects such as anti-aging and prevention / improvement of spots. In addition, since the lemon balm plant is also used as a food, it is highly safe, so cosmetics, external preparations for skin, pharmaceuticals, foods and drinks and feeds containing the lemon balm plant or its extract are expected to be highly safe. be able to.

Claims (9)

Component (A) astaxanthins, and
Ingredient (B) A composition containing the Labiatae genus Lemon balm plant or an extract thereof.   The composition according to claim 1, further comprising collagen as component (C). The composition according to claim 1 or 2, wherein the collagen of component (C) is one or more selected from water-soluble collagen, hydrolyzed collagen, atelocollagen, and succinyl atelocollagen.   The composition of any one of Claims 1-3 which suppresses decomposition | disassembly of the said component (A) astaxanthin by light.   Cosmetics, a skin external preparation, or a quasi-drug containing the composition of any one of Claims 1-4.   Use of the composition according to any one of claims 1 to 6 for the production of a cosmetic, an external preparation for skin, a quasi-drug, a pharmaceutical, a food or drink, or a feed.   A stabilizer for astaxanthins, comprising as an active ingredient a Lembiaceae Lemon balm plant or an extract thereof.   A method for stabilizing astaxanthins using a Lemaceae Lemon balm plant or an extract thereof.   The stabilization method of Claim 8 which suppresses photolysis of astaxanthin.