JP2017002010A - Cosmetic compositions - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide cosmetic compositions exhibiting a higher skin-beautifying effect by aiming at protection of fibroblasts simultaneously with improvement of blood flow.SOLUTION: A cosmetic composition is characterized by containing as active ingredients Pinus bark extract and a particular ingredient, such as at least one plant material selected from Peucedanum japonicum, Kaempferia parviflora, and Cocos nucifera.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition containing a pine bark extract and specific other ingredients as active ingredients.

  In recent years, research on anti-aging that prevents skin aging such as wrinkles has been actively conducted, and collagen and hyaluronic acid have attracted attention. It is known that this collagen and hyaluronic acid are produced by fibroblasts constituting the dermis of the skin.

  Examples of compositions that focus on the production of such collagen and hyaluronic acid include, for example, a collagen production promoter (see Patent Document 1) characterized by comprising β-glucan containing β1,3D glucan, and cardamom A cosmetic composition for promoting collagen production (see Patent Document 2) containing an extract of the above, and a composition for promoting hyaluronic acid production (see Patent Document 3), characterized by containing an extract extracted from a plant body of the family Chironomidae ) Has been proposed.

  On the other hand, in modern society, poor blood circulation (blood flow) is often a problem due to various factors such as lack of exercise and autonomic dysfunction due to stress. This poor blood flow (decrease in blood flow) causes symptoms such as skin dullness and swelling. Therefore, there is a need for measures to improve blood flow not only from a health perspective but also from a cosmetic perspective. Yes.

  Examples of such a composition for improving blood flow include a hyssop extract (see Patent Document 4), a composition containing a cassis concentrate and an amino acid or an organic acid (see Patent Document 5), a chamomile extract ( Patent Document 6) has been proposed.

Japanese Patent Laying-Open No. 2015-71588 JP-A-2015-48338 JP 2013-155140 A JP 2006-8575 A JP 2004-262878 A JP 2005-68069 A

  An object of the present invention is to provide a cosmetic composition that exhibits a higher skin beautifying effect by simultaneously protecting fibroblasts and improving blood flow.

  The present inventors diligently investigated and researched both from the viewpoint of fibroblast protection and blood flow improvement regarding beauty, and by combining pine bark extract and specific other ingredients, a high fibroblast protection effect was obtained. At the same time, it was found that a high blood flow improvement effect was obtained, and the present invention was completed. That is, by combining a pine bark extract with a specific other component having little or no fibroblast protective ability and / or blood flow improving ability, fibroblast protective effect and blood flow improving effect can be obtained. We found that both could be improved synergistically.

That is, the present invention relates to a cosmetic composition comprising as an active ingredient a pine bark extract and at least one component selected from the group consisting of the following (a) to (e).
(A) at least one plant material selected from long life grass, black ginger, and coconut palm (b) at least one sweetener selected from maltose, trehalose, reduced palatinose, and reduced maltose (c) alanine, asparagine, asparagine At least one amino acid selected from acids, cysteine, isoleucine, methionine, phenylalanine, proline, tryptophan, and tyrosine (d) chlorogenic acid (e) at least one vitamin selected from vitamin C, niacin, folic acid, and zinc Minerals

  The cosmetic composition of the present invention has a skin beautifying action, a skin protecting action, and a blood flow improving action, and can be a skin beautifying agent used for skin beautification.

  The cosmetic composition of the present invention may be obtained by adding an active ingredient comprising a pine bark extract and at least one component selected from the group consisting of (a) to (e).

  The cosmetic composition of the present invention is preferably for oral use, and is preferably a tablet, capsule, powder, granule, or liquid.

  The present invention also relates to a cosmetic method (excluding medical practice) characterized by ingesting the cosmetic composition of the present invention.

  According to the cosmetic composition of the present invention, fibroblasts that contribute to the production of collagen and hyaluronic acid can be protected, and the skin can be protected from damages such as ultraviolet rays, preventing wrinkles and preventing aging (anti-aging), etc. Beauty effects such as prevention and improvement of symptoms such as dull skin and swelling can be obtained. Further, the blood flow improving action makes it possible to spread the fibroblast protective action component widely, and to obtain a higher skin-beautifying effect.

The upper diagram shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + long life grass) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the beauty composition (pine bark extract + long life grass) of this invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper figure is a figure showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + black ginger) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + black ginger) of this invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper diagram shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + coconut) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + coconut) of invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + malt sugar) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + maltose) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + trehalose) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + trehalose) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper diagram shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + reduced palatinose) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + reduced palatinose) of this invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper diagram shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + reduced maltose) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the beauty composition (pine bark extract + reduced maltose) of this invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + alanine) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + alanine) of invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + asparagine) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + asparagine) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper figure is a figure showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + aspartic acid) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + aspartic acid) of this invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + cysteine) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + cysteine) of invention to pig pulmonary artery vascular endothelial cells (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + isoleucine) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + isoleucine) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + methionine) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + methionine) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + phenylalanine) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + phenylalanine) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + proline) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + proline) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + tryptophan) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + tryptophan) of invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + tyrosine) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + tyrosine) of invention to pig pulmonary artery vascular endothelial cells (PPAEC). The upper diagram shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + chlorogenic acid) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + chlorogenic acid) of this invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper diagram shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + vitamin C) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount at the time of applying the cosmetic composition (pine bark extract + vitamin C) of this invention to a pig pulmonary artery vascular endothelial cell (PPAEC). The upper diagram shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + niacin) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + niacin) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The upper figure shows the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + folic acid) is applied to normal human fibroblasts (NHDF). It is a figure which shows the result of NO production amount when the cosmetic composition (pine bark extract + folic acid) of the invention is applied to porcine pulmonary artery vascular endothelial cells (PPAEC). The above figure is a figure which shows the result of the cell survival rate with respect to oxidative stress at the time of applying the beauty composition (pine bark extract + zinc (zinc gluconate)) of this invention to normal human fibroblasts (NHDF). The figure below shows the results of NO production when the cosmetic composition of the present invention (pine bark extract + zinc (zinc gluconate)) is applied to porcine pulmonary artery vascular endothelial cells (PPAEC).

  The cosmetic composition of the present invention contains a pine bark extract and at least one component selected from the group consisting of the following (a) to (e) (hereinafter sometimes referred to as other components) as active ingredients. It is characterized by that. That is, the following (a) to (e) components used together with the pine bark extract may be one type (for example, the (a) component), or two or more types may be combined. When two or more other components are used in combination, it is preferable to combine the pine bark extract and other components having a high synergistic effect.

  The cosmetic composition of the present invention protects fibroblasts more effectively from oxidative stress by the synergistic effect of the combination of such active ingredients, suppresses the decrease in production of collagen and hyaluronic acid, and prevents skin damage due to ultraviolet rays and the like. It can reduce and effectively prevent or improve symptoms such as wrinkles and aging. In addition, the cosmetic composition of the present invention can more effectively relax muscles around the blood vessels to dilate blood vessels and increase blood flow due to the synergistic effect of the combination of active ingredients. Can be effectively prevented or ameliorated. In addition, this improvement in blood flow makes it possible to spread the fibroblast protective action to the end of the body and obtain a higher skin-beautifying effect.

  The cosmetic composition of the present invention has a skin beautifying action, a skin protecting action, and a blood flow improving action. The cosmetic composition of the present invention can be a skin beautifying agent used for skin beautification, a skin protecting agent used for skin protection, or a blood flow improving agent used for improving blood flow. The cosmetic composition of the present invention can also be used as a collagen production promoter or hyaluronic acid production promoter.

[Pine bark extract]
The pine bark extract as an active ingredient of the cosmetic composition of the present invention contains proanthocyanidins as one of main ingredients. Proanthocyanidins are a group of compounds composed of a condensation polymer having a degree of polymerization of 2 or more having flavan-3-ol and / or flavan-3,4-diol as a structural unit.

  Examples of the raw material of the pine bark extract used in the present invention include Pinus Martina, larch, Japanese black pine, Japanese red pine, Japanese pine, Japanese pine, Chinese pine, Japanese pine, Ryukyu pine, Japanese pine, Japanese pine, Japanese white pine, etc. Of these, French coastal pine is preferred. The obtained extract may be concentrated or dried as necessary to form a liquid, a paste, or a powder. Moreover, a commercial item can be used for a pine bark extract, for example, the pine bark extract by Toyo Shinyaku Co., Ltd. can be used.

  The pine bark extract used in the present invention preferably contains a condensation polymer having a degree of polymerization of 2 or more as proanthocyanidins. In particular, proanthocyanidins containing a large amount of a condensation polymer having a low degree of polymerization are preferred. Examples of the condensation polymer having a low degree of polymerization include a condensation polymer having a degree of polymerization of 2 to 30 (2 to 30 mer), and a condensation polymer having a degree of polymerization of 2 to 10 (2 to 10 mer). Preferably, a condensation polymer having a degree of polymerization of 2 to 4 (2 to 4 mer) is more preferable. In the present specification, a polymer having a polymerization degree of 2 to 4 is referred to as OPC (oligomeric proanthocyanidin). The pine bark extract used in the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and still more preferably 40% by mass or more.

  The pine bark extract is obtained by extracting pine bark with water or an organic solvent. When water is used, warm water or hot water is preferably used. Organic solvents used for extraction include foods or drugs such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, etc. An organic solvent acceptable for the production of is preferably used. These water and organic solvents may be used alone or in combination of two or more solvents. In particular, water, ethanol, hydrous ethanol and the like are preferably used.

  The method for extracting the pine bark extract from the pine bark is not particularly limited, and for example, extraction methods such as a warm extraction method and a supercritical fluid extraction method can be used. In the method for extracting pine bark extract, a plurality of extraction methods may be combined.

[Other ingredients]
(A) Plant material In the cosmetic composition of the present invention, it is preferable to use at least one plant material selected from long-life grass, black ginger and coconut as an active ingredient together with the pine bark extract.

  These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc. In addition to the plant materials themselves (including dried products), pulverized products, juices In addition, a processed plant material such as an extract can be used. Examples of the pulverized product include powder and granules. The juice or extract may be liquid, but can also be used as a paste or dry powder. The extract can be obtained by extraction using an appropriate solvent. As the solvent, for example, water (hot water, hot water), ethanol, hydrous ethanol can be used. As these plant materials, commercially available products can be used.

  Long-life grass is a perennial plant that is also referred to as a button-bowl, which belongs to the genus Azalea. As a site | part used as a plant raw material of this invention, a leaf and a root are preferable.

  Black ginger is named Kaempferia Parviflora, and is a plant belonging to the genus Banturmeric that grows naturally in Southeast Asia. As a site | part used as a plant raw material of this invention, a rhizome is preferable.

  Coco palm is a plant of the palm family, and fruit (endosperm) is preferable as a site used as a plant material of the present invention.

(B) Sweetener In the cosmetic composition of the present invention, it is preferable to use at least one sweetener selected from malt sugar, trehalose, reduced palatinose, and reduced maltose as an active ingredient together with the pine bark extract. The sweetener of the present invention means a sweetener and is not limited to the purpose of use as a sweetener.

(C) Amino acid In the cosmetic composition of the present invention, together with the pine bark extract, the active ingredient is at least one selected from alanine, asparagine, aspartic acid, cysteine, isoleucine, methionine, phenylalanine, proline, tryptophan, and tyrosine. It is preferable to use amino acids (including salts). Examples of the salt include sodium salt and hydrochloride.

(D) Chlorogenic acid In the cosmetic composition of the present invention, chlorogenic acid is preferably used as an active ingredient together with the pine bark extract.

(E) Vitamins and minerals In the cosmetic composition of the present invention, at least one vitamin / mineral selected from vitamin C, niacin, folic acid, and zinc is used as an active ingredient together with the pine bark extract. preferable. In addition, zinc as minerals of this invention contains the form of the compound containing zinc.

  The cosmetic composition of the present invention is not particularly limited as long as it contains a pine bark extract and other predetermined components and can be distinguished from other products as a product in terms of use in cosmetics. For example, functions of beauty effects such as a skin beautifying effect, a skin protecting effect, a blood flow improving effect, a collagen production promoting effect, a hyaluronic acid production promoting effect on any of the main body, packaging, instructions and promotional materials of the product according to the present invention The indication that there is is included in the scope of the present invention. For example, so-called health foods such as pharmaceuticals (including quasi-drugs), cosmetics, functional foods for which the indication of efficacy has been approved by prescribed organizations such as foods for specified health use, functional nutritional foods, functional indication foods Can be mentioned. In so-called health foods, “barrier to the skin”, “protect the skin”, “for those worried about stains”, “for people worried about wrinkles”, “for skin that does not lose stress”, “ultraviolet rays” ”For skin that does n’t lose”, “happy for beauty”, “happy for skin”, etc., “improves circulation”, “for those who care about skin color”, “bearing eyes” For example, “is displayed” or the like can be exemplified. The cosmetic composition of the present invention can be taken regardless of gender and age as long as it is a person who cares about the above effects, but can enjoy the effects of the cosmetic composition of the present invention more effectively. It is desirable for women to take, and more desirably for postmenopausal women.

  The cosmetic composition of the present invention can be used for oral use or external use. The external preparation is not particularly limited as long as it is applied to the skin, scalp and the like, and the form is an ointment, cream, gel, lotion, emulsion, pack, poultice, etc. The form of skin external preparations, injections, etc. can be mentioned.

  Further, when the cosmetic composition of the present invention is used as an oral preparation, examples of the form thereof include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, and the like. Examples include a shape agent, a round agent, a paste agent, a cream agent, a caplet agent, a gel agent, a chewable agent, and a stick agent. Among these, the forms of tablets, capsules, powders, granules, and liquids are particularly preferable. Specifically, supplements, container-packed beverages filled in PET bottles, cans, bottles, etc., instant powder beverages and instant granule beverages for dissolving and drinking in water (hot water), milk, fruit juice, green juice, etc. Can be illustrated. These are preferable in that they can be easily taken at the time of eating and the like, and can enhance palatability.

  The contents of the pine bark extract and other components (active ingredients) in the cosmetic composition of the present invention may be appropriately contained within the range where the effects are exerted.

  In general, when the cosmetic composition of the present invention is a pharmaceutical or a supplement (tablet, capsule), the active ingredient is preferably contained in an amount of 0.01 to 100% by mass in terms of dry mass, It is more preferable that 0.1-85 mass% is contained, and it is further more preferable that 0.5-70 mass% is contained.

  When the cosmetic composition of the present invention is a packaged beverage (liquid), the active ingredient is preferably contained in an amount of 0.01 to 15% by mass in terms of dry mass, and 0.03 to 12.5. More preferably, it is contained in an amount of 0.05% by mass, and more preferably 0.05-10% by mass.

  Moreover, when the cosmetic composition of the present invention is an instant powder beverage (powder) or an instant granule beverage (granule), the active ingredient is contained in an amount of 0.1 to 80% by mass in terms of dry mass. It is preferable that 0.5 to 70% by mass is included, and 1 to 60% by mass is more preferable.

  In order to exhibit the effect of the present invention more effectively, the active ingredient is preferably contained in an amount of 80% by mass or more of the entire cosmetic composition of the present invention (excluding moisture) in terms of dry mass, and 90% by mass or more. It is more preferably contained, more preferably 95% by mass or more, and particularly preferably 100% by mass. Furthermore, when the cosmetic composition of the present invention contains any one of the components (a) to (c), the contained component is preferably composed only of the active ingredients in the present invention. That is, when the cosmetic composition of the present invention contains, for example, the component (a) (plant material), it is preferable that the cosmetic composition does not contain plant materials other than long-lived grass, black ginger and coconut palm.

  The intake amount of the cosmetic composition of the present invention is not particularly limited, but from the viewpoint of exerting the effects of the present invention more remarkably, the intake amount of the active ingredient per day is 0.1 mg / day or more. Ingestion is preferable, intake is preferably 1 mg / day or more, more preferably 10 mg / day or more, and intake is particularly preferably 40 mg / day or more. . Although the upper limit in particular is not restrict | limited, For example, it is 4 g / day, Preferably it is 2 g / day.

  The cosmetic composition of the present invention can be stored in one container or divided into a plurality of containers, for example, as one day so that the daily intake becomes the above-mentioned intake.

  The blending mass ratio of the pine bark extract and other components is preferably in the range of 0.5: 1 to 70: 1 and in the range of 0.75: 1 to 60: 1 in terms of dry mass. Is more preferably in the range of 1: 1 to 60: 1, and particularly preferably in the range of 1: 1 to 50: 1. When the blending ratio of the pine bark extract and other components is within the above range, the effects of the present invention can be more effectively exhibited.

  The cosmetic composition of the present invention can be produced by a known formulation method by adding components other than the active ingredient acceptable as an oral preparation or an external preparation, if necessary.

  In addition, the cosmetic composition as an oral preparation of the present invention can include a cosmetic composition (beauty food) obtained by adding an active ingredient to a food in addition to a cosmetic composition containing an active ingredient. For example, a food in which the content of the active ingredient of the present invention is increased compared to a normal food (including a natural food), or a food in which an active ingredient is added to a food that does not normally contain the active ingredient of the present invention Can be mentioned. For the addition of the active ingredients, the respective ingredients may be added separately, may be added simultaneously, or may be added together with other ingredients other than the active ingredients.

  Examples of the beauty food of the present invention include carbonated beverages, nutritional beverages, fruit beverages, lactic acid beverages, smoothies, green juice and other frozen desserts; ice cream, ice sherbet, shaved ice and other frozen desserts; soba, udon, harusame, Chinese noodles, Noodles such as instant noodles; sweets such as candy, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread; fishery and livestock processed foods such as kamaboko, ham, sausage; processing Dairy products such as milk, fermented milk, yogurt; salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and other fats and processed foods; seasonings such as sauces, soy sauce; curry, stew, oyakodon, o Rice cakes, miscellaneous dishes, Chinese rice cakes, rice cakes, tempura, beef rice bowls, hayashi rice, omelet rice, oden, Mabodorf, Child, Shumai, mention may be made of hamburger, meatballs, various sources, retort pouch foods such as various soups and the like.

  The cosmetic method of the present invention is characterized by ingesting the above-described cosmetic composition of the present invention, but does not include medical practice. The cosmetic composition of the present invention can be ingested regardless of gender and age, but since the effect of the cosmetic composition of the present invention can be enjoyed more effectively, it is desirable for women to ingest it. It is more desirable to have females ingested. Examples of the method of the present invention include a method of obtaining a beauty effect by providing the beauty food of the present invention at a restaurant such as a restaurant. Moreover, in an esthetic salon etc., the method of providing the beauty food of this invention can be mentioned as one of the treatment.

Hereinafter, the present invention will be described based on examples.
[Example 1] (Confirmation of skin protective effect)
1. Cell culture (1) Normal human fibroblasts (NHDF) were cultured in 10% FBS-DMEM using a 75 cm ² flask in a 37 ° C., 5% CO ₂ incubator.
(2) Cells suspended by trypsin treatment were seeded from a 75 cm ² flask to each well of 96 well plate at a cell density of 1.0 × 10 ⁴ cells / well.
(3) Pre-culture for 24 hours in a 37 ° C., 5% CO ₂ incubator.
(4) After removing the medium from each well, 200 μL of a test substance-containing medium prepared to a predetermined concentration was added and cultured in a CO ₂ incubator for 24 hours.

  In addition, about the combined sample of a pine bark extract and another component, the test substance containing culture medium of 2 times concentration was prepared, respectively, and it was set as the target density | concentration by adding 100 microliters. For the control, the test substance dissolved in DMSO was prepared using 10% FBS-DMEM containing 0.25% DMSO, and 10% FBS-DMEM was used for other test substances. Prepared.

  For the pine bark extract, a hot water extract (dry powder) of the bark of French coastal pine was used. As other components used together with the pine bark extract, substances shown in Tables 1 to 5 were used.

(5) After incubation for 24 hours, 50 μL of hydrogen peroxide prepared to 2.5 mM with serum-free DMEM was added (final concentration 500 μM). Control (+) was obtained by adding hydrogen peroxide to Control.

2. Measurement of cell viability against oxidative stress (1) 90 minutes after addition of hydrogen peroxide solution, the medium was removed from the cultured cells, washed once with PBS 200 μL / well, and diluted 30-fold with serum-free DMEM 150 μL of Cell Counting Kit-8 solution was added.
(2) The sample was allowed to stand in a 37 ° C., 5% CO ₂ incubator for appropriate color development, and the absorbance at 450 nm was measured.
(3) The cell viability (% of control) for control (+) was calculated based on the obtained data.

  % of control = (Data sample-Data blank) / (Data control-Data blank) x 100

[Example 2] (Confirmation of blood flow improvement effect)
1. Cell culture
(1) Porcine pulmonary artery vascular endothelial cells (PPAEC) were cultured with 10% FBS-RPMI 1640 using a 75 cm ² flask in a 37 ° C., 5% CO ₂ incubator.
(2) Cells suspended by trypsin treatment were seeded from a 75 cm ² flask to each well of a 96-well plate at a cell density of 1.0 × 10 ⁴ cells / well.
(3) Pre-culture for 48 hours in a 37 ° C., 5% CO ₂ incubator.
(4) After removing the medium from each well, 100 μL of a test substance-containing medium prepared to a predetermined concentration with 4% FBS-αMEM was added and cultured in a CO ₂ incubator for 16 hours.

  In addition, about the combined use sample of a pine bark extract and another component, it was set as the target density | concentration by adding 50 microliters of test substance containing culture media of 2 times each. For the control, the test substance dissolved in DMSO was prepared using 4% FBS-αMEM containing 0.25% DMSO, and 4% FBS-αMEM was used for other test substances. Prepared.

  For the pine bark extract, a hot water extract (dry powder) of the bark of French coastal pine was used. As other components used together with the pine bark extract, substances shown in Tables 1 to 5 were used.

2. Evaluation of NO production
(1) After 16 hours of culturing, 50 μL of the culture supernatant was collected in a 1.5 mL sample tube, and N = 3 samples were mixed.
(2) Centrifugation was performed at 4 ° C. and 2000 g for 3 minutes, and the supernatant was transferred to another 1.5 mL Eppendorf tube.
(3) The NO concentration (μM) in the sample of (2) was measured using Nitrate / Nitrite Assay Kit, Fluorometric (manufactured by Cayman). Note that NO produced from vascular endothelial cells has the effect of relaxing the muscles around the blood vessels and dilating the blood vessels.
(4) “% of control” was calculated based on the calculated NO concentration.

% Of control
= NO concentration of sample (μM) / NO concentration of control (μM) × 100

  The results of Example 1 and Example 2 are shown in Tables 1 to 5 and FIGS.

  From the left, the top of each figure shows the results of “control (hydrogen peroxide addition)”, “single addition of pine bark extract”, “single addition of other components”, “addition of pine bark extract + other components” Represents. The lower figure of each figure represents the results of “control (no addition)”, “single addition of pine bark extract”, “single addition of other components”, “pine bark extract + addition of other components” from the left. Moreover, the numerical value in description of the graph lower part shows sample concentration, for example, "pine_10" of the graph of the upper figure of FIG. 1 shows the pine bark extract 10 (microgram / mL). The concentration unit of the additive component is μg / mL in all graphs. The vertical axis represents “% of control”.

For long-lived grasses, dry ground powder was used.
For black ginger, a 60% ethanol extract (dried powder) of rhizomes of black ginger was used.
About coconut, after drying commercially available coconut milk, what remove | excluded oil was used.
For maqui berry, a commercially available fruit dry powder was used.

  As shown in Tables 1 to 5 and FIGS. 1 to 22, by combining the pine bark extract and specific other components of the present invention, the cell viability of fibroblasts against oxidative stress (upper figure), In addition, both NO production from vascular endothelial cells (lower figure) increased synergistically. Therefore, according to the cosmetic composition of the present invention, fibroblasts are protected and blood flow is improved, and a very excellent cosmetic effect can be obtained.

Example 3 (Manufacture of tablets)
A tablet (500 mg) comprising the following components was produced.
Reduced maltose 5% by mass
Collagen peptide 15% by mass
Soy protein 15% by mass
Starch 15% by mass
Pine bark extract 8% by mass
Vitamin C 1% by mass
Vitamin B1 1% by mass
Vitamin B2 1% by mass
Vitamin B6 1% by mass
1% by mass of folic acid
Zinc gluconate 1% by mass
Cellulose 2% by mass
Calcium chloride 2% by mass
Calcium stearate 1% by mass

[Example 4] (Manufacture of capsules)
The following mixture was enclosed in a soft capsule to produce a capsule.
Hyaluronic acid 150mg
Maltose 50mg
Reduced maltose 50mg
Pine bark extract 20mg
Silicon dioxide 10mg
Vitamin E 0.1mg
Niacin 0.5mg
Asparagine 0.25mg
Aspartic acid 0.25mg
Magnesium 1mg

[Example 5] (Production of granules)
The following ingredients were mixed to produce granules by a conventional method.
Fructooligosaccharide 20g
Isomaltooligosaccharide 20g
Calcium stearate 2g
1g of silicon dioxide
Pine bark extract 0.4g
Chlorogenic acid 0.1g
Niacin 0.01g
Zinc gluconate 0.001g

[Example 6] (Production of instant powder)
The following components were mixed to produce an instant powder (10 g) by a conventional method.
Trehalose 0.1g
Aspartame 0.1g
Sucrose fatty acid ester 2g
Pine bark extract 0.4g
Alanine 0.01g
Arginine 0.02g
Glycine 0.01g
0.01g isoleucine
Methionine 0.01g
Phenylalanine 0.01g
Proline 0.01g
Tryptophan 0.01g
Tyrosine 0.01g
Valine 0.02g
Glutamine 0.01g
Zinc gluconate 0.001g
0.001 g of iron chloride
Fragrance 0.001g
Calcium stearate balance

[Example 7] (Production of liquid preparation)
A liquid (50 mL) comprising the following components was produced.
Porcine placenta 2000mg
Pine bark extract 400mg
Maltose 150mg
Asparagine 150mg
Aspartic acid 150mg
Isoleucine 50mg
Proline 50mg
Tryptophan 25mg
Vitamin C 0.001% by mass
Vitamin B1 0.001% by mass
Vitamin B6 0.001% by mass
Water remaining

  Since the cosmetic composition of the present invention has a high cosmetic effect and can be used as an oral preparation or an external preparation, the industrial utility of the present invention is high.

Claims (9)

A cosmetic composition comprising as an active ingredient a pine bark extract and at least one component selected from the group consisting of the following (a) to (e):
(A) at least one plant material selected from long life grass, black ginger, and coconut palm (b) at least one sweetener selected from maltose, trehalose, reduced palatinose, and reduced maltose (c) alanine, asparagine, asparagine At least one amino acid selected from acids, cysteine, isoleucine, methionine, phenylalanine, proline, tryptophan, and tyrosine (d) chlorogenic acid (e) at least one vitamin selected from vitamin C, niacin, folic acid, and zinc Minerals   The cosmetic composition according to claim 1, which has a beautifying action.   The cosmetic composition according to claim 1 or 2, which has a skin protecting action.   The cosmetic composition according to any one of claims 1 to 3, which has a blood flow improving action.   The cosmetic composition according to any one of claims 1 to 4, wherein the cosmetic composition is a skin beautifier used for skin beautification.   The beauty according to any one of claims 1 to 5, obtained by adding an active ingredient comprising a pine bark extract and at least one ingredient selected from the group consisting of (a) to (e). Composition.   The cosmetic composition according to any one of claims 1 to 6, wherein the cosmetic composition is for oral use.   The cosmetic composition according to claim 7, which is a tablet, capsule, powder, granule, or liquid.   A beauty method (however, excluding medical practice) characterized by ingesting the cosmetic composition according to any one of claims 1 to 8.