JP2016519950A5 - - Google Patents
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- Publication number
- JP2016519950A5 JP2016519950A5 JP2016516145A JP2016516145A JP2016519950A5 JP 2016519950 A5 JP2016519950 A5 JP 2016519950A5 JP 2016516145 A JP2016516145 A JP 2016516145A JP 2016516145 A JP2016516145 A JP 2016516145A JP 2016519950 A5 JP2016519950 A5 JP 2016519950A5
- Authority
- JP
- Japan
- Prior art keywords
- intein
- fusion protein
- seq
- peptide
- mxe gyra
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000017730 intein-mediated protein splicing Effects 0.000 claims 13
- 238000000034 method Methods 0.000 claims 12
- 108020001507 fusion proteins Proteins 0.000 claims 10
- 102000037865 fusion proteins Human genes 0.000 claims 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims 10
- 101800002094 Mxe GyrA intein Proteins 0.000 claims 7
- 235000001014 amino acid Nutrition 0.000 claims 3
- 235000018417 cysteine Nutrition 0.000 claims 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 3
- 230000035772 mutation Effects 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 108010054814 DNA Gyrase Proteins 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 1
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 210000001124 body fluid Anatomy 0.000 claims 1
- 239000010839 body fluid Substances 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- -1 linker amino acids Chemical class 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 150000003573 thiols Chemical class 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13170009 | 2013-05-31 | ||
| EP13170009.8 | 2013-05-31 | ||
| US201361833046P | 2013-06-10 | 2013-06-10 | |
| US61/833,046 | 2013-06-10 | ||
| PCT/EP2014/061048 WO2014191455A1 (en) | 2013-05-31 | 2014-05-28 | Methods for producing peptides using engineered inteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016519950A JP2016519950A (ja) | 2016-07-11 |
| JP2016519950A5 true JP2016519950A5 (https=) | 2017-07-13 |
Family
ID=48536725
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016516145A Withdrawn JP2016519950A (ja) | 2013-05-31 | 2014-05-28 | 操作されたインテインを使用してペプチドを生産する方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9670257B2 (https=) |
| EP (1) | EP3003357A1 (https=) |
| JP (1) | JP2016519950A (https=) |
| CN (1) | CN105263509A (https=) |
| WO (1) | WO2014191455A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9771412B2 (en) * | 2014-10-31 | 2017-09-26 | Wisconsin Alumni Research Foundation | Engineered intein for improved production of protein-intein fusions |
| PL3215614T3 (pl) | 2014-11-03 | 2019-12-31 | Merck Patent Gmbh | Rozpuszczalne białka fuzyjne inteiny i sposoby oczyszczania biocząsteczek |
| WO2020125707A1 (en) | 2018-12-19 | 2020-06-25 | Tsinghua University | Mtu δi-cm intein variant and the use thereof |
| US20220332757A1 (en) * | 2019-09-09 | 2022-10-20 | Wuhan Yzy Biopharma Co., Ltd. | Split intein and preparation method for recombinant polypeptide using the same |
| GB202300816D0 (en) | 2023-01-19 | 2023-03-08 | Isomerase Therapeutics Ltd | Novel method |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9708918D0 (en) | 1997-05-01 | 1997-06-25 | Ppl Therapeutics Scotland Ltd | Methods |
| EP1090132A1 (en) | 1998-06-26 | 2001-04-11 | PPL Therapeutics (Scotland) Limited | Methods for production of recombinant polypeptides |
| JP4749548B2 (ja) | 1998-09-30 | 2011-08-17 | ニユー・イングランド・バイオレイブズ・インコーポレイテツド | インテイン媒介ペプチド連結 |
| FI20021726A0 (fi) * | 2002-09-27 | 2002-09-27 | Ctt Cancer Targeting Tech Oy | Menetelmä peptidien tuottamiseksi |
| US9249181B2 (en) | 2010-09-13 | 2016-02-02 | Amylin Pharmaceuticals, Llc | C-terminal amidation of polypeptides |
| WO2012100176A2 (en) * | 2011-01-20 | 2012-07-26 | University Of Rochester | Macrocyclic compounds with a hybrid peptidic/non-peptidic backbone and methods for their preparation |
| KR102096534B1 (ko) | 2011-09-28 | 2020-04-03 | 에라 바이오테크, 에스.에이. | 분할된 인테인 및 그의 이용 |
-
2014
- 2014-05-28 US US14/893,349 patent/US9670257B2/en not_active Expired - Fee Related
- 2014-05-28 CN CN201480030331.8A patent/CN105263509A/zh not_active Withdrawn
- 2014-05-28 JP JP2016516145A patent/JP2016519950A/ja not_active Withdrawn
- 2014-05-28 EP EP14727800.6A patent/EP3003357A1/en not_active Withdrawn
- 2014-05-28 WO PCT/EP2014/061048 patent/WO2014191455A1/en not_active Ceased
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