CN105263509A - 使用工程改造的内含肽生产肽的方法 - Google Patents
使用工程改造的内含肽生产肽的方法 Download PDFInfo
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- CN105263509A CN105263509A CN201480030331.8A CN201480030331A CN105263509A CN 105263509 A CN105263509 A CN 105263509A CN 201480030331 A CN201480030331 A CN 201480030331A CN 105263509 A CN105263509 A CN 105263509A
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- 229960004666 glucagon Drugs 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 230000003284 homeostatic effect Effects 0.000 description 1
- 230000020245 homoiothermy Effects 0.000 description 1
- 230000006658 host protein synthesis Effects 0.000 description 1
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- 230000000148 hypercalcaemia Effects 0.000 description 1
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- 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 description 1
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- NONJJLVGHLVQQM-JHXYUMNGSA-N phenethicillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C(C)OC1=CC=CC=C1 NONJJLVGHLVQQM-JHXYUMNGSA-N 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 235000008729 phenylalanine Nutrition 0.000 description 1
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- WROMPOXWARCANT-UHFFFAOYSA-N tfa trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F.OC(=O)C(F)(F)F WROMPOXWARCANT-UHFFFAOYSA-N 0.000 description 1
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- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13170009 | 2013-05-31 | ||
| EP13170009.8 | 2013-05-31 | ||
| US201361833046P | 2013-06-10 | 2013-06-10 | |
| US61/833046 | 2013-06-10 | ||
| PCT/EP2014/061048 WO2014191455A1 (en) | 2013-05-31 | 2014-05-28 | Methods for producing peptides using engineered inteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105263509A true CN105263509A (zh) | 2016-01-20 |
Family
ID=48536725
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480030331.8A Withdrawn CN105263509A (zh) | 2013-05-31 | 2014-05-28 | 使用工程改造的内含肽生产肽的方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9670257B2 (https=) |
| EP (1) | EP3003357A1 (https=) |
| JP (1) | JP2016519950A (https=) |
| CN (1) | CN105263509A (https=) |
| WO (1) | WO2014191455A1 (https=) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021047559A1 (zh) * | 2019-09-09 | 2021-03-18 | 武汉友芝友生物制药有限公司 | 一种断裂型内含肽、使用其的重组多肽的制备方法 |
| US11136360B2 (en) | 2018-12-19 | 2021-10-05 | Tsinghua University | Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9771412B2 (en) * | 2014-10-31 | 2017-09-26 | Wisconsin Alumni Research Foundation | Engineered intein for improved production of protein-intein fusions |
| PL3215614T3 (pl) | 2014-11-03 | 2019-12-31 | Merck Patent Gmbh | Rozpuszczalne białka fuzyjne inteiny i sposoby oczyszczania biocząsteczek |
| GB202300816D0 (en) | 2023-01-19 | 2023-03-08 | Isomerase Therapeutics Ltd | Novel method |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9708918D0 (en) | 1997-05-01 | 1997-06-25 | Ppl Therapeutics Scotland Ltd | Methods |
| EP1090132A1 (en) | 1998-06-26 | 2001-04-11 | PPL Therapeutics (Scotland) Limited | Methods for production of recombinant polypeptides |
| JP4749548B2 (ja) | 1998-09-30 | 2011-08-17 | ニユー・イングランド・バイオレイブズ・インコーポレイテツド | インテイン媒介ペプチド連結 |
| FI20021726A0 (fi) * | 2002-09-27 | 2002-09-27 | Ctt Cancer Targeting Tech Oy | Menetelmä peptidien tuottamiseksi |
| US9249181B2 (en) | 2010-09-13 | 2016-02-02 | Amylin Pharmaceuticals, Llc | C-terminal amidation of polypeptides |
| WO2012100176A2 (en) * | 2011-01-20 | 2012-07-26 | University Of Rochester | Macrocyclic compounds with a hybrid peptidic/non-peptidic backbone and methods for their preparation |
| KR102096534B1 (ko) | 2011-09-28 | 2020-04-03 | 에라 바이오테크, 에스.에이. | 분할된 인테인 및 그의 이용 |
-
2014
- 2014-05-28 US US14/893,349 patent/US9670257B2/en not_active Expired - Fee Related
- 2014-05-28 CN CN201480030331.8A patent/CN105263509A/zh not_active Withdrawn
- 2014-05-28 JP JP2016516145A patent/JP2016519950A/ja not_active Withdrawn
- 2014-05-28 EP EP14727800.6A patent/EP3003357A1/en not_active Withdrawn
- 2014-05-28 WO PCT/EP2014/061048 patent/WO2014191455A1/en not_active Ceased
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11136360B2 (en) | 2018-12-19 | 2021-10-05 | Tsinghua University | Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification |
| WO2021047559A1 (zh) * | 2019-09-09 | 2021-03-18 | 武汉友芝友生物制药有限公司 | 一种断裂型内含肽、使用其的重组多肽的制备方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014191455A1 (en) | 2014-12-04 |
| US9670257B2 (en) | 2017-06-06 |
| JP2016519950A (ja) | 2016-07-11 |
| US20160096872A1 (en) | 2016-04-07 |
| EP3003357A1 (en) | 2016-04-13 |
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