JP2016518855A - 融合プロテアーゼ - Google Patents

融合プロテアーゼ Download PDF

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Publication number
JP2016518855A
JP2016518855A JP2016514440A JP2016514440A JP2016518855A JP 2016518855 A JP2016518855 A JP 2016518855A JP 2016514440 A JP2016514440 A JP 2016514440A JP 2016514440 A JP2016514440 A JP 2016514440A JP 2016518855 A JP2016518855 A JP 2016518855A
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JP
Japan
Prior art keywords
protease
protein
fusion
bifunctional fusion
bifunctional
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JP2016514440A
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English (en)
Japanese (ja)
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JP2016518855A5 (enExample
Inventor
アラン・クリスチャン・ショー
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Novo Nordisk AS
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Novo Nordisk AS
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Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of JP2016518855A publication Critical patent/JP2016518855A/ja
Publication of JP2016518855A5 publication Critical patent/JP2016518855A5/ja
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14—Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)
    • C12Y304/14011—Xaa-Pro dipeptidyl-peptidase (3.4.14.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22028—Picornain 3C (3.4.22.28)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016514440A 2013-05-24 2014-05-23 融合プロテアーゼ Withdrawn JP2016518855A (ja)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
EP13169173.5 2013-05-24
EP13169173 2013-05-24
EP13171191.3 2013-06-10
EP13171191 2013-06-10
US201361834100P 2013-06-12 2013-06-12
US61/834,100 2013-06-12
EP13194053 2013-11-22
EP13194053.8 2013-11-22
US201361909412P 2013-11-27 2013-11-27
US61/909,412 2013-11-27
PCT/EP2014/060696 WO2014187974A1 (en) 2013-05-24 2014-05-23 Fusion protease

Publications (2)

Publication Number Publication Date
JP2016518855A true JP2016518855A (ja) 2016-06-30
JP2016518855A5 JP2016518855A5 (enExample) 2017-06-15

Family

ID=51932984

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016514440A Withdrawn JP2016518855A (ja) 2013-05-24 2014-05-23 融合プロテアーゼ

Country Status (5)

Country Link
US (1) US20160122793A1 (enExample)
EP (1) EP3004369A1 (enExample)
JP (1) JP2016518855A (enExample)
CN (1) CN105247067A (enExample)
WO (2) WO2014187960A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160060311A1 (en) 2014-08-27 2016-03-03 Daewoong Jo Development of Protein-Based Biotherapeutics That Penetrates Cell-Membrane and Induces Anti-Lung Cancer Effect - Improved Cell-Permeable Suppressor of Cytokine Signaling (iCP-SOCS3) Proteins, Polynucleotides Encoding the Same, and Anti-Lung Cancer Compositions Comprising the Same
CN110128540B (zh) * 2019-04-18 2021-12-14 江南大学 一种基于便携式血糖仪的二抗
CN110592057B (zh) * 2019-09-27 2022-01-28 昆明理工大学 嵌合裂解酶ILTphg和编码此酶的多核苷酸
CN112011497B (zh) * 2020-08-28 2022-06-07 上海交通大学 分泌表达猪源性抗产肠毒素大肠杆菌k88菌毛单链抗体的重组乳酸乳球菌及其制备方法
US20250122489A1 (en) * 2023-10-16 2025-04-17 Zhenyu Zhu Protein Thermostability Reduction by Tag Fusion and Its Usage

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56166200A (en) * 1980-02-29 1981-12-21 Univ California Idiosyncratically cutting linker
JPH05501360A (ja) * 1990-02-03 1993-03-18 マックス―プランク―ゲゼルシャフト　ズー　フォーデルング　デール　ウィッセンシャフテン　エー．ヴェー． ＩｇＡプロテアーゼによる組み換えタンパク質の酵素的切断法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56166200A (en) * 1980-02-29 1981-12-21 Univ California Idiosyncratically cutting linker
JPH05501360A (ja) * 1990-02-03 1993-03-18 マックス―プランク―ゲゼルシャフト　ズー　フォーデルング　デール　ウィッセンシャフテン　エー．ヴェー． ＩｇＡプロテアーゼによる組み換えタンパク質の酵素的切断法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIO/TECHNOLOGY, vol. 3, JPN6018009699, 1985, pages 821 - 823, ISSN: 0003760154 *
J. BACTERIOL., vol. 187, no. 2, JPN6018009701, January 2005 (2005-01-01), pages 795 - 799, ISSN: 0003760157 *
PROTEIN EXPR. PURIF., vol. 24, no. 3, JPN5016005891, 1 April 2002 (2002-04-01), pages 530 - 538, ISSN: 0003760156 *
PROTEIN EXPR. PURIF., vol. 80, no. 2, JPN6018009700, December 2011 (2011-12-01), pages 283 - 293, ISSN: 0003760155 *

Also Published As

Publication number Publication date
WO2014187974A1 (en) 2014-11-27
US20160122793A1 (en) 2016-05-05
WO2014187960A1 (en) 2014-11-27
EP3004369A1 (en) 2016-04-13
CN105247067A (zh) 2016-01-13

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