JP2016502407A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2016502407A5 JP2016502407A5 JP2015544195A JP2015544195A JP2016502407A5 JP 2016502407 A5 JP2016502407 A5 JP 2016502407A5 JP 2015544195 A JP2015544195 A JP 2015544195A JP 2015544195 A JP2015544195 A JP 2015544195A JP 2016502407 A5 JP2016502407 A5 JP 2016502407A5
- Authority
- JP
- Japan
- Prior art keywords
- promoter
- recombinase
- gene
- site
- selectable marker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims description 58
- 210000001082 somatic cell Anatomy 0.000 claims description 56
- 239000003550 marker Substances 0.000 claims description 48
- 108010091086 Recombinases Proteins 0.000 claims description 45
- 102000018120 Recombinases Human genes 0.000 claims description 41
- 108010052160 Site-specific recombinase Proteins 0.000 claims description 37
- 230000006798 recombination Effects 0.000 claims description 36
- 238000005215 recombination Methods 0.000 claims description 36
- 210000000287 oocyte Anatomy 0.000 claims description 27
- 238000011144 upstream manufacturing Methods 0.000 claims description 26
- 210000004027 cell Anatomy 0.000 claims description 25
- 239000002773 nucleotide Substances 0.000 claims description 22
- 125000003729 nucleotide group Chemical group 0.000 claims description 22
- 108700028369 Alleles Proteins 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 210000002950 fibroblast Anatomy 0.000 claims description 10
- 241000282898 Sus scrofa Species 0.000 claims description 8
- 210000002459 blastocyst Anatomy 0.000 claims description 8
- 230000035897 transcription Effects 0.000 claims description 8
- 238000013518 transcription Methods 0.000 claims description 8
- 241000283690 Bos taurus Species 0.000 claims description 6
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims description 6
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims description 6
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 claims description 6
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims description 6
- 101150111214 lin-28 gene Proteins 0.000 claims description 6
- 210000001789 adipocyte Anatomy 0.000 claims description 4
- 210000000601 blood cell Anatomy 0.000 claims description 4
- 210000002449 bone cell Anatomy 0.000 claims description 4
- 210000003494 hepatocyte Anatomy 0.000 claims description 4
- 210000001161 mammalian embryo Anatomy 0.000 claims description 4
- 244000309715 mini pig Species 0.000 claims description 4
- 210000000663 muscle cell Anatomy 0.000 claims description 4
- 210000002569 neuron Anatomy 0.000 claims description 4
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 4
- 210000004927 skin cell Anatomy 0.000 claims description 4
- 241000283707 Capra Species 0.000 claims description 2
- 241000283074 Equus asinus Species 0.000 claims description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 230000003213 activating effect Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 230000013020 embryo development Effects 0.000 claims description 2
- 210000003292 kidney cell Anatomy 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 210000004291 uterus Anatomy 0.000 claims description 2
- 241000282887 Suidae Species 0.000 claims 2
- 241000271566 Aves Species 0.000 claims 1
- 241000283086 Equidae Species 0.000 claims 1
- 241000699670 Mus sp. Species 0.000 claims 1
- 241000700159 Rattus Species 0.000 claims 1
- 230000008685 targeting Effects 0.000 description 3
- 241000283073 Equus caballus Species 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261730771P | 2012-11-28 | 2012-11-28 | |
| US61/730,771 | 2012-11-28 | ||
| PCT/US2013/072088 WO2014085501A1 (en) | 2012-11-28 | 2013-11-26 | Cloned non-human animals free of selective markers |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016226816A Division JP6227747B2 (ja) | 2012-11-28 | 2016-11-22 | 選択マーカーを含まないクローン化された非ヒト動物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016502407A JP2016502407A (ja) | 2016-01-28 |
| JP2016502407A5 true JP2016502407A5 (https=) | 2017-01-12 |
| JP6154479B2 JP6154479B2 (ja) | 2017-06-28 |
Family
ID=49725406
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015544195A Expired - Fee Related JP6154479B2 (ja) | 2012-11-28 | 2013-11-26 | 選択マーカーを含まないクローン化された非ヒト動物 |
| JP2016226816A Expired - Fee Related JP6227747B2 (ja) | 2012-11-28 | 2016-11-22 | 選択マーカーを含まないクローン化された非ヒト動物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016226816A Expired - Fee Related JP6227747B2 (ja) | 2012-11-28 | 2016-11-22 | 選択マーカーを含まないクローン化された非ヒト動物 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20140221734A1 (https=) |
| EP (1) | EP2925122A1 (https=) |
| JP (2) | JP6154479B2 (https=) |
| KR (1) | KR20150089004A (https=) |
| CN (1) | CN104918484A (https=) |
| AU (1) | AU2013352235A1 (https=) |
| BR (1) | BR112015012408A2 (https=) |
| CA (1) | CA2891163A1 (https=) |
| HK (1) | HK1211177A1 (https=) |
| IL (1) | IL238771A0 (https=) |
| MX (1) | MX353279B (https=) |
| MY (1) | MY193790A (https=) |
| RU (1) | RU2015122802A (https=) |
| SG (1) | SG11201503752SA (https=) |
| WO (1) | WO2014085501A1 (https=) |
| ZA (1) | ZA201503305B (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3988649B1 (en) * | 2013-09-18 | 2024-11-27 | Kymab Limited | Methods, cells and organisms |
| JP6929527B2 (ja) | 2015-03-04 | 2021-09-01 | 株式会社ポル・メド・テック | 安定した表現型を示す疾患モデルブタおよびその作製方法 |
| CN105200086A (zh) * | 2015-10-22 | 2015-12-30 | 中国农业科学院北京畜牧兽医研究所 | 一种无选择标记的多基因复合性状基因工程猪的制备方法 |
| KR20250141836A (ko) * | 2015-11-04 | 2025-09-29 | 페이트 세러퓨틱스, 인코포레이티드 | 만능 세포의 유전자 조작 |
| CA3099441A1 (en) | 2018-06-13 | 2019-12-19 | Regeneron Pharmaceuticals, Inc. | A rodent model of fibrodysplasia ossificans progressiva |
| CN109504708A (zh) * | 2018-12-03 | 2019-03-22 | 江苏集萃药康生物科技有限公司 | 一种筛选标记自我删除的基因打靶载体及方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1375654A3 (en) * | 1998-11-02 | 2008-01-16 | GTC Biotherapeutics, Inc. | Transgenic and cloned mammals |
| US7049480B1 (en) * | 2000-09-01 | 2006-05-23 | Avigenics, Inc. | Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy |
| EP1811023A4 (en) * | 2004-09-29 | 2009-04-29 | Dainippon Sumitomo Pharma Co | SPECIFICALLY EXPRESSED IN ES CELLS AND USE THEREOF |
| WO2009121999A1 (es) * | 2008-04-04 | 2009-10-08 | Consejo Superior De Investigaciones Científicas | Promotor especifico de celulas troncales |
| US8518392B2 (en) * | 2009-08-14 | 2013-08-27 | Regeneron Pharmaceuticals, Inc. | Promoter-regulated differentiation-dependent self-deleting cassette |
| CN102628061A (zh) * | 2012-04-09 | 2012-08-08 | 西北农林科技大学 | 一种hbd3 乳腺特异性表达载体及构建的重组细胞 |
-
2013
- 2013-11-26 RU RU2015122802A patent/RU2015122802A/ru not_active Application Discontinuation
- 2013-11-26 US US14/091,207 patent/US20140221734A1/en not_active Abandoned
- 2013-11-26 MY MYPI2015001216A patent/MY193790A/en unknown
- 2013-11-26 HK HK15112184.4A patent/HK1211177A1/xx unknown
- 2013-11-26 CN CN201380060720.0A patent/CN104918484A/zh active Pending
- 2013-11-26 EP EP13802213.2A patent/EP2925122A1/en not_active Withdrawn
- 2013-11-26 JP JP2015544195A patent/JP6154479B2/ja not_active Expired - Fee Related
- 2013-11-26 KR KR1020157012263A patent/KR20150089004A/ko not_active Withdrawn
- 2013-11-26 MX MX2015006810A patent/MX353279B/es active IP Right Grant
- 2013-11-26 WO PCT/US2013/072088 patent/WO2014085501A1/en not_active Ceased
- 2013-11-26 BR BR112015012408A patent/BR112015012408A2/pt not_active IP Right Cessation
- 2013-11-26 CA CA2891163A patent/CA2891163A1/en not_active Abandoned
- 2013-11-26 SG SG11201503752SA patent/SG11201503752SA/en unknown
- 2013-11-26 AU AU2013352235A patent/AU2013352235A1/en not_active Abandoned
-
2015
- 2015-05-12 IL IL238771A patent/IL238771A0/en unknown
- 2015-05-12 ZA ZA2015/03305A patent/ZA201503305B/en unknown
-
2016
- 2016-11-22 JP JP2016226816A patent/JP6227747B2/ja not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2685914C1 (ru) | Способы и композиции для направленной модификации генома | |
| JP2015533284A5 (https=) | ||
| JP2016502407A5 (https=) | ||
| Moghaddassi et al. | TALEN-mediated modification of the bovine genome for large-scale production of human serum albumin | |
| ES2731437T3 (es) | Métodos y composiciones para la modificación genética dirigida mediante el uso de pares de ARN guías | |
| ES2947714T3 (es) | Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso | |
| RU2676708C2 (ru) | Направленная модификация генома крысы | |
| CN105101787B (zh) | 遗传修饰的动物及其制备方法 | |
| CN105142396A (zh) | 缺角家畜 | |
| Galli et al. | Somatic cell nuclear transfer and transgenesis in large animals: current and future insights | |
| JP6227747B2 (ja) | 選択マーカーを含まないクローン化された非ヒト動物 | |
| IL319209A (en) | Non-human animals containing a humanized ASGR1 locus | |
| Laible | Production of transgenic livestock: overview of transgenic technologies | |
| RU2018103093A (ru) | Гуманизированная скелетная мышца | |
| Wang et al. | Versatile generation of precise gene edits in bovines using SEGCPN | |
| RU2017132331A (ru) | Etv2 и его применения | |
| RU2018103234A (ru) | Гуманизированная мышца сердца | |
| WO2017175745A1 (ja) | 生殖細胞欠損動物を用いる遺伝子改変動物の作製方法 | |
| JP7007734B2 (ja) | 条件付きノックアウト動物の製造方法 | |
| Hussain et al. | CRISPR/Cas System and Stem Cell Editing: Prospects and Possibilities in Veterinary Sciences | |
| CN102226199A (zh) | 用于生产外源蛋白的山羊β-乳球蛋白基因座打靶载体构建方法 | |
| CN121204148A (zh) | 一种高表达人源化ace2动物模型的制备方法 | |
| Bicanic et al. | Regional differences in dendritic morphology of medium spiny striatal neurons in Foxp2 mice are influenced by substitution at position T302N | |
| Young et al. | Genome editing in mice using CRISPR/Cas | |
| Akbulut | Transgenic animal technology with genetic engineering and its use with advantages and disadvantages. |