JP2016114491A - Automatic analysis device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an automatic analysis device with which it is possible to dispense a sample with high accuracy without causing a processing speed to decrease.SOLUTION: An automatic analysis device comprises: a sample dispensing probe 19 for sucking in a sample inside a sample container 11 and performing dispensation by discharging a dispensation amount of the sample smaller than or equal to an upper-limit amount VM that is set in inspection items for each of the inspection items into a reaction vessel 17; and an analysis control unit for controlling a sample dispensing operation by the sample dispensing probe 19. When a plurality of inspection items are set to the sample including an inspection item in which a dispensation amount smaller than or equal to a minute amount VL that is smaller than the upper-limit amount VM, the analysis control unit causes the sample dispensing probe 19 to suck in a total amount of the sample in which a total of dispensation amounts set in two or more inspection items including an inspection item in which the dispensation amount smaller than or equal to the minute amount VL is set is smaller than or equal to the upper-limit amount VM.SELECTED DRAWING: Figure 2

Description

  Embodiments described herein relate generally to an automatic analyzer that dispenses a sample collected from a subject and analyzes components contained in the sample.

  Tests using automatic analyzers target biochemical test items, immunological test items, etc., and optically detect changes in color and turbidity caused by the reaction of the mixture of the sample collected from the specimen and the reagent used to analyze each test item. Measure automatically. By this measurement, analytical data represented by the concentrations of various test item components in the sample, enzyme activities, and the like are generated.

  In the automatic analyzer, the amount of sample used for analysis is set for each inspection item, and the inspection item is set for each sample according to the inspection. Then, the amount of the sample stored in the sample container is dispensed into the reaction container by the sample dispensing probe for each inspection item.

  In recent years, the amount of samples and reagents has been reduced in consideration of the burden on the subject and the reduction of running costs. In particular, the amount of sample dispensed into the reaction vessel is very small compared to that of the reagent. Therefore, a technique for accurately dispensing a small amount of sample into the reaction vessel is required.

  In the automatic analyzer, the number of inspection items and samples to be analyzed is increasing, and the speed of each process is being increased. Therefore, it is necessary to increase the operating speed of the sample dispensing probe.

JP 2014-70901 A

  However, in sample dispensing, if the operating speed of the sample dispensing probe is increased, there is a problem that the dispensing accuracy of a small amount of sample is lowered.

  The embodiment has been made to solve the above-described problems, and an object thereof is to provide an automatic analyzer that can accurately dispense a sample without reducing the processing speed.

  In order to achieve the above object, an automatic analyzer according to an embodiment dispenses a sample and a reagent into a reaction vessel, and measures the mixed solution of the sample and the reagent in the reaction vessel. A dispensing probe for aspirating the sample inside and dispensing the sample into the reaction container in such an amount that the dispensing amount set in this inspection item is less than or equal to the upper limit amount for each inspection item; A plurality of inspection items including an inspection item in which a dispensing amount equal to or less than a predetermined amount smaller than the upper limit amount is set in the sample, and a dispensing amount equal to or less than the predetermined amount is set among the plurality of inspection items. And an analysis control unit that causes the dispensing probe to suck a total amount of the sample in which the total amount of dispensing set in two or more inspection items including the inspection item is equal to or less than the upper limit amount. And

The block diagram which shows the structure of the automatic analyzer which concerns on embodiment. The perspective view which shows the structure of the analysis part which concerns on embodiment. The top view which shows arrangement | positioning of the reaction container hold | maintained at the sample dispensing probe which concerns on embodiment, the sample dispensing arm, the sample container hold | maintained at the sample table, and the reaction table. The figure which shows the dispensing amount set to each test | inspection item which concerns on embodiment. The figure which shows the dispensing process performed based on each test | inspection item set to the sample which concerns on embodiment, and this test | inspection item, and a timing chart. The flowchart which shows the 1st and 2nd dispensing process which concerns on embodiment. The figure which shows the sample of the dispensing amount attracted | sucked in the sample dispensing probe which concerns on embodiment. The figure which shows the 1st timing chart which concerns on embodiment. The figure which shows the 2nd timing chart which concerns on embodiment. The figure which shows the dispensing process performed based on each test | inspection item set to the sample which concerns on embodiment, and this test | inspection item, and a timing chart. The figure which shows the 1st and 2nd dispensing process which concerns on embodiment. The figure which shows the sample of the total amount attracted | sucked in the sample dispensing probe which concerns on embodiment. The figure which shows the 1st timing chart which concerns on embodiment. The figure which shows the 2nd timing chart which concerns on embodiment. The figure which shows the dispensing process performed based on each test | inspection item set to the sample which concerns on embodiment, and this test | inspection item, and a timing chart. The figure which shows the sample of the total amount attracted | sucked in the sample dispensing probe which concerns on embodiment. The flowchart which shows the 3rd dispensing process which concerns on embodiment. The figure which shows the sample of the total amount attracted | sucked in the sample dispensing probe which concerns on embodiment. The figure which shows the 3rd timing chart which concerns on embodiment.

  Hereinafter, embodiments will be described with reference to the drawings.

  FIG. 1 is a block diagram illustrating a configuration of an automatic analyzer according to the embodiment. This automatic analyzer 100 dispenses a sample such as a standard sample or a test sample and a reagent for analyzing each inspection item, and measures a mixed solution of the sample and the reagent to generate standard data and test data. Part 10 is provided. Further, a drive unit 40 that drives various units of the analysis unit 10 and an analysis control unit 41 that controls the drive unit 40 are provided.

  The automatic analyzer 100 also includes a data processing unit 60 that generates calibration data and analysis data from the standard data and test data generated by the analysis unit 10, and calibration data and analysis data generated by the data processing unit 60. And an output unit 70 for outputting. Also, the operation unit 80 for inputting analysis parameters such as the amount of sample (dispensing amount) and the amount of reagent for each inspection item that can be inspected, the analysis control unit 41, the data processing unit 60, and the output unit 70 are integrated. And a system control unit 90 that performs control.

  FIG. 2 is a perspective view showing the configuration of the analysis unit 10. The analysis unit 10 includes a sample container 11 for storing a sample such as a standard sample or a test sample, and a sample table 12 for holding the sample container 11 so as to be movable. In addition, for example, a reagent container 13 for storing the first reagent and the two reagent system first reagent and the two reagent system second reagent for analysis of each test item, and two reagent racks that hold the reagent container 13 movably 14.

  In addition, a reagent storage 15 that cools the first reagent in the reagent container 13 held in the reagent rack 14 and a reagent storage 16 that cools the second reagent in the reagent container 13 held in the reagent rack 14 are provided. Yes. In addition, a plurality of reaction vessels 17 arranged on the circumference and a reaction table 18 that holds the reaction vessels 17 in a rotatable manner are provided.

  In addition, a sample dispensing probe 19 that performs dispensing to suck the sample in the sample container 11 held on the sample table 12 and discharge the sample into the reaction container 17, and a sample that holds the sample dispensing probe 19 movably. A dispensing arm 20 is provided. In addition, a first reagent dispensing probe 21 for aspirating the first reagent in the reagent container 13 held in the reagent rack 14 and discharging it into the reaction container 17 into which the sample has been dispensed, and the first reagent A first reagent dispensing arm 22 that holds the one reagent dispensing probe 21 in a movable manner is provided.

  Moreover, the 1st stirring element 23 which stirs the liquid mixture of the sample and the 1st reagent dispensed to the reaction container 17, and the 1st stirring arm 24 which hold | maintains the 1st stirring element 23 so that a movement is possible are provided. A second reagent dispensing probe 25 for aspirating the second reagent in the reagent container 13 held in the reagent rack 14 and dispensing it into the reaction container 17 into which the first reagent has been dispensed; A second reagent dispensing arm 26 that holds the second reagent dispensing probe 25 in a movable manner is provided.

  In addition, a second stirrer 27 that stirs the sample dispensed in the reaction vessel 17, a mixed solution of the first reagent and the second reagent, and a second stirrer arm 28 that holds the second stirrer 27 movably. It has. Further, a measuring unit 29 that optically measures the mixed liquid in the reaction vessel 17 and a cleaning nozzle 30 that cleans the inside of the reaction vessel 17 that has been measured by the measuring unit 29 are provided. Then, the measurement unit 29 irradiates the reaction container 17 with light, and, for example, changes in absorbance and absorbance are detected based on a detection signal that detects light transmitted through the mixed solution containing the standard sample and the test sample in the reaction container 17. Generate standard data and test data expressed in quantities.

  The drive unit 40 illustrated in FIG. 1 drives the sample table 12 of the analysis unit 10 to move the sample container 11. Further, each reagent rack 14 is driven to move the reagent container 13. Further, the reaction table 18 is driven to rotate and move each reaction vessel 17. Further, the sample dispensing arm 20, the first reagent dispensing arm 22, the second reagent dispensing arm 26, the first stirring arm 24, and the second stirring arm 28 are driven, respectively, so that the sample dispensing probe 19 and the first reagent are driven. The dispensing probe 21, the second reagent dispensing probe 25, the first stirring bar 23, and the second stirring bar 27 are moved. Further, the cleaning nozzle 30 is moved.

  Further, the drive unit 40 drives the sample dispensing pump connected to the sample dispensing probe 19 by suction. By this suction drive, the sample dispensing pump performs a suction operation to cause the sample dispensing probe 19 to suck the sample in the sample container 11. Further, the sample dispensing pump is driven to discharge. By this discharge driving, the sample dispensing pump discharges and causes the sample dispensing probe 19 to discharge the sample into the reaction container 17.

  The analysis control unit 41 controls the drive unit 40. Then, based on the analysis parameters of each inspection item input from the operation unit 80, each reaction vessel 17 of the analysis unit 10 is rotated and moved for each cycle with one analysis cycle time TC as one cycle, which is different from that before the movement. Stop at position. Further, the sample dispensing probe 19 sucks the sample in the sample container 17 and discharges it into the reaction container 17 for each cycle.

  The data processing unit 60 processes the standard data and test data generated by the measurement unit 29 of the analysis unit 10 to generate calibration data and analysis data for each inspection item, and is generated by the calculation unit 61. And a data storage unit 62 for storing standard data and analysis data. And the calculating part 61 produces | generates calibration data based on the standard data of each test | inspection item. Moreover, based on the calibration data and test data of each test item, analysis data indicated by a concentration value and an activity value is generated.

  The output unit 70 includes a printing unit 71 that prints out calibration data and analysis data generated by the calculation unit 61 of the data processing unit 60 and a display unit 72 that displays and outputs the calibration data. Then, the display unit 72 displays analysis parameters such as an amount (dispensing amount) that is equal to or less than the maximum amount (upper limit amount VM) in which the sample in the sample container 11 can be dispensed into one reaction container 17 for each inspection item. Displays the analysis parameter setting screen for setting. In addition, for each sample, an identification information such as a name and ID for identifying the sample, and an inspection item setting screen for setting an inspection item to be inspected are displayed.

  The operation unit 80 includes input devices such as a keyboard, a mouse, a button, and a touch key panel, and performs input for setting analysis parameters for each test item, identification information for each sample, input for setting test items, and the like. Do.

  The system control unit 90 includes a CPU and a storage circuit, and stores input information such as analysis parameters of each inspection item, sample identification information, and inspection item information input from the operation unit 80 in the storage circuit. Based on the input information, the analysis control unit 41, the data processing unit 60, and the output unit 70 are integrated to control the entire system.

  Next, the operation of the sample dispensing probe 19, the sample dispensing arm 20, the sample container 11 held on the sample table 12, and the reaction container 17 held on the reaction table 18 related to sample dispensing will be described.

  FIG. 3 is a plan view showing the arrangement of the sample dispensing probe 19, the sample dispensing arm 20, the sample container 11 held on the sample table 12 and the reaction container 17 held on the reaction table 18.

  A sample dispensing arm 20 is disposed between the sample table 12 and the reaction table 18. A cleaning tank 31 for cleaning the sample dispensing probe 19 is disposed between the sample table 12 and the reaction table 18.

  The sample dispensing probe 19 is located between the cleaning tank 31 and the upper stop position above the sample container 11 stopped at the sample container position P10, and the upper stop position above the reaction container 17 stopped at the reaction container position C10. In between and between the upper stop position and the cleaning tank 31, an arcuate trajectory is drawn and moved in the horizontal arrow R1 direction and in the arrow R2 direction opposite to the R1 direction.

  The sample dispensing probe 19 descends from the upper stop position of the sample container 11 that stops at the sample container position P10, and stops at the suction position where the sample in the sample container 11 can be sucked. Then, after the sample is sucked at the suction position, the sample is raised to the upper stop position. Moreover, it descends from the upper stop position of the reaction container 17 that stops at the reaction container position C10, and stops at the discharge position where the sample in the reaction container 17 can be discharged. Then, after the dispensing amount of the sample set for this inspection item is discharged for each inspection item at the discharge position, the sample rises to the upper stop position.

  The analysis control unit 41 sets a plurality of inspection items including an inspection item in which a dispensing amount equal to or less than a small amount VL smaller than the upper limit amount VM is set in the sample in the sample container 11 stopped at the sample container position P10. If there are two or more inspection items including inspection items in which a small amount VL or less is set, a plurality of inspection items including inspection items in which a small amount VL or less is set The sample dispensing probe 19 sucks the total amount of the sample in which the total amount of dispensing set in the inspection item is equal to or less than the upper limit amount VM. In addition, when only the inspection item in which the dispensing amount exceeding the minute amount VL is set for the sample in the sample container 11 stopped at the sample container position P10, the dispensing amount exceeding the minute amount VL is set. The sample is aspirated.

FIG. 4 is a diagram showing the dispensing amount set for each inspection item.
For each inspection item such as inspection item A, inspection item B, inspection item C, inspection item D, and inspection item E that can be inspected by the automatic analyzer 100, the sample dispensing probe 16 has a reaction container 17 with one analysis cycle time CT. A dispensing amount that can be discharged is set. In the inspection item A, for example, a dispensing amount Va that is larger than a minute amount VL having an upper limit of 1.0 μL and smaller than the upper limit amount VM is set. In addition, in the inspection item B, a dispensed amount Vb of a minute amount VL or less is set. Further, in the inspection item C, a dispensing amount Vc that is the upper limit amount VM is set. In addition, in the inspection item D, a dispensing amount Vd that is less than or equal to a minute amount VL is set. In the inspection item E, a dispensing amount Ve that is larger than the minute amount VL and smaller than the upper limit amount VM is set.

  Hereinafter, an example of sample dispensing in the automatic analyzer 100 will be described with reference to FIGS. 1 to 19. Below, the timing of each dispensing process and dispensing operation performed based on each test | inspection item set to each sample P1, P2, P3 is demonstrated.

First, dispensing of the sample P1 will be described.
FIG. 5 is a diagram showing each inspection item set on the sample P1, and a dispensing process and timing chart executed based on the inspection item. Three inspection items A, C, and E are set for the sample P1. As described above, in the case where the dispensing control unit 41 includes only the inspection items A, C, and E in which the dispensing amounts Va, Vc, and Ve exceeding the minute amount VL are set, the analysis control unit 41 performs the first and second dispensing operations. Based on the injection steps 51 and 52 and the first and second timing charts TC1 and TC2, for example, the sample P1 is dispensed in the order of the inspection item A, the inspection item C, and the inspection item E.

  In the first cycle of dispensing the sample P1, based on the first dispensing step 51 and the first timing chart TC1, the sample P1 having the dispensing amount Va set in the first inspection item A is sucked. Dispensing to be discharged into the reaction container 17 is executed. Further, in each cycle after the second cycle, the dispensing of the sample P1 is executed based on the second dispensing step 52 and the second timing chart TC2.

  Then, in the second cycle, the dispensing amount Vc set in the second inspection item C is sucked and the dispensing amount Vc sample P1 is discharged into the reaction container 17 is executed. Furthermore, the dispensing amount Ve sample P1 set in the inspection item E in the third cycle is aspirated, and the dispensing amount Ve sample P1 is discharged into the reaction container 17 to be executed.

FIG. 6 is a flowchart showing the first and second dispensing steps 51 and 52.
The first dispensing process 51 includes steps S1 to S12. Then, in the first cycle of dispensing the sample P1, the sample dispensing probe 19 is washed in the washing tank 31 (step S1).

  The sample dispensing probe 19 is moved from the cleaning tank 31 toward the upper stop position of the sample container 11 that stops at the sample container position P10 by driving the drive unit 40 that rotates the sample dispensing arm 20 in the R2 direction ( Step S2).

  As shown in FIG. 7A, the sample dispensing probe 19 aspirates a predetermined amount of air to prevent mixing of the pressure transmission medium held inside the sample P1 in accordance with the movement from the cleaning tank 31. (Step S3).

  The sample dispensing probe 19 that has moved to the upper stop position of the sample container 11 that stops at the sample container position P10 can suck the sample P1 in the sample container 11 by driving the drive unit 40 that lowers the sample dispensing arm 20. Descends to the correct suction position (step S4).

  As shown in FIG. 7B, the sample dispensing probe 19 lowered to the suction position sucks the sample P1 having a dummy amount VD for preventing the pressure transmission medium from being mixed into the sample P1 for each inspection item ( Step S5). Subsequently, the sample P1 with the dispensing amount Va for the inspection item A is aspirated (step S6).

  The sample dispensing probe 19 that has aspirated the sample P1 is raised from the discharge position to the upper stop position of the sample container 11 by driving the drive unit 40 that raises the sample dispensing arm 20 (step S7).

  The sample dispensing probe 19 that has risen to the upper stop position of the sample container 11 is stopped at the reaction container position C10 from the upper stop position of the sample container 11 by driving the drive unit 40 that rotates the sample dispensing arm 20 in the R1 direction. The reaction container 17 is moved to the upper stop position (step S8).

  The sample dispensing probe 19 moved to the upper stop position of the reaction vessel 17 can discharge the sample P1 in the reaction vessel 17 stopped at the reaction vessel position C10 by driving the drive unit 40 that lowers the sample dispensing arm 20. For example, the sample dispensing probe 19 descends to the discharge position where the lower end of the sample dispensing probe 19 contacts the bottom of the inner surface of the reaction vessel 17 (step S9).

  The sample dispensing probe 19 lowered to the discharge position discharges the sample P1 of the dispensing amount Va into the reaction container 17 at the reaction container position C10 (Step S10).

  The sample dispensing probe 19 that has ejected the sample P1 is raised to the upper stop position of the reaction vessel 17 by driving the drive unit 40 that raises the sample dispensing arm 20 (step S11).

  The sample dispensing probe 19 that has risen to the upper stop position of the reaction vessel 17 moves to the cleaning tank 31 by driving the drive unit 40 that rotates the sample dispensing arm 20 in the R2 direction (step S12).

  The second dispensing process 52 includes steps S2, S4 and steps S6 to S12 obtained by removing steps S1, S3, and S5 from the first dispensing process 51. In step S6 of the second dispensing step 52 in the second cycle, the sample dispensing probe 19 that has been lowered to the suction position sucks the sample P1 with the dispensing amount Vc for the inspection item C. In step S10, the sample dispensing probe 19 lowered to the discharge position discharges the sample P1 having the dispensing amount Vc into the reaction container 17 at the reaction container position C10.

  In step S6 of the second dispensing process 52 in the third cycle, the sample dispensing probe 19 lowered to the suction position sucks the sample P1 with the dispensing amount Ve for the inspection item E. Further, in step S10, the sample dispensing probe 19 lowered to the discharge position discharges the sample P1 of the dispensing amount Ve into the reaction container 17 at the reaction container position C10.

  FIG. 8 is a diagram showing a first timing chart TC1. This first timing chart TC1 shows the timing of “rotation” and “stop” when the reaction table 18 stops after one analysis cycle time CT, and the timing TS1 when the first dispensing step 51 is executed. To TS12. Then, a time Td1 is allocated from the rise of the timing TS9 to the fall of the timing TS11.

  In the first dispensing process 51, step S1 is executed at timing TS1, and step S2 is executed at timing TS2. Further, step S3 is executed at timing TS3, and step S4 is executed at timing TS4. Further, step S5 is executed at timing TS5, and step S6 is executed at timing TS6. Further, step S7 is executed at timing TS7, and step S8 is executed at timing TS8. Further, step S9 is executed at timing TS9, and step S10 is executed at timing TS10. Further, step S11 is executed at timing TS11, and step S12 is executed at timing TS12.

  Then, at time Td1 from the rise of timing TS9 to the fall of timing TS11, the sample dispensing probe 19 descends from the upper stop position of the reaction vessel 17 stopped at the reaction vessel position C10 to the discharge position, and dispenses at the discharge position. After the amount Va of sample P1 is discharged, it rises to the upper stop position.

  FIG. 9 is a diagram illustrating a second timing chart TC2. The second timing chart TC2 is different from the first timing chart TC1 in that timings TS1, TS3, and TS5 are excluded. As a result, the second timing chart TC2 shows the timings of “rotation” and “stop” at which the reaction table 18 stops after rotating, and the timings TS2, TS4 and timings TS6 through TS2 at which the second dispensing step 52 is executed. TS12. Then, time Td1 is allocated from the rise of timing TS9 to the fall of timing TS11.

  In the second dispensing step 52, step S2 is executed at timing TS2, and step S4 is executed at timing TS4. Further, step S6 is executed at timing TS6, and step S7 is executed at timing TS7. Further, step S8 is executed at timing TS8, and step S9 is executed at timing TS9. Further, step S10 is executed at timing TS10, and step S11 is executed at timing TS11. Further, step S12 is executed at timing TS12.

  Then, at time Td1 from the rise of timing TS9 to the fall of timing TS11, the sample dispensing probe 19 descends from the upper stop position of the reaction container 17 stopped at the reaction container position C10 to the discharge position, and at the discharge position, After the injection amount Vc, Ve of sample P1 is discharged, it rises to the upper stop position.

Next, dispensing of the sample P2 will be described.
FIG. 10 is a diagram showing each inspection item set on the sample P2 and a dispensing process and timing chart executed based on the inspection item. Two inspection items B and D are set for the sample P2. Thus, the inspection item B in which the dispensing amount Vb is set to a minute amount VL or less and the inspection item D in which the dispensing amount Vd is set are included, and the sum of the dispensing amount Vb and the dispensing amount Vd is increased. When the total amount is equal to or less than the limit VM (Vb + Vd), the analysis control unit 41 determines the sample P2 based on the first and second dispensing steps 51a and 52a and the first and second timing charts TC1a and TC2a. Execute dispensing of.

  Based on the first dispensing process 51a and the first timing chart TC1a in the first cycle of dispensing the sample P2, the sample P2 of the total amount (Vb + Vd) is sucked for the two inspection items B and D, Dispensing for discharging the sample P2 of the dispensing amount Vb set in the first inspection item B of the two inspection items B and D into the reaction container 17 is executed. In addition, based on the second dispensing step 52a and the second timing chart TC2a in the second cycle, the dispensing amount Vd set in the second inspection item D of the two inspection items B and D. Dispensing for discharging the sample P2 into the reaction vessel 17 is executed.

  FIG. 11 is a flowchart showing the first and second dispensing steps 51a and 52a. The first dispensing process 51a differs from the first dispensing process 51 in that step S6 is replaced with step S6a and step S12 is excluded. Thereby, the 1st dispensing process 51a is comprised by step S1 thru | or S5, step S6a, and step S7 thru | or S11.

  In step S6a of the first dispensing process 51a in the first cycle, the sample dispensing probe 19 lowered to the suction position sucks the total amount (Vb + Vd) of the sample P2 as shown in FIG. In step S10, the sample dispensing probe 19 that has been lowered to the discharge position uses the sample P2 having the dispensing amount Vb set in the first inspection item B of the two inspection items B and D as the reaction container. Discharge into the reaction vessel 17 stopped at position C10. In step S11, the sample dispensing probe 19 that has ejected the sample P2 rises to the upper stop position of the reaction container 17 that stops at the reaction container position C10. After rising to the upper stop position, in the second cycle, the reaction vessel 17 waits at the upper stop position until a reaction vessel 17 different from the reaction vessel 17 stopped in the first cycle is stopped at the reaction vessel position C10.

  The second dispensing process 52a differs from the second dispensing process 52 in that steps S2, S4 and steps S6 to S8 are excluded. Thereby, the 2nd dispensing process 52a is comprised by step S9 thru | or S12.

  In step S9 of the second dispensing process 52a in the second cycle, the sample dispensing probe 19 that has waited at the upper stop position in step S11 of the first dispensing process 51a descends from the upper stop position. In step S10, the sample dispensing probe 19 lowered to the discharge position uses the sample P2 of the dispensing amount Vd set in the second inspection item D of the two inspection items B and D as the reaction container position C10. It discharges in the reaction container 17 stopped by.

  FIG. 13 shows the first timing chart TC1a. The first timing chart TC1a differs from the first timing chart TC1 in that the timing TS12 is excluded and the timings TS10 and TS11 are moved backward without changing the time from the rising edge to the falling edge of the timing. This is a point replaced with the moved timings TS10a and TS11a. As a result, the first timing chart TC1a includes “rotation” and “stop” of the reaction table 18, and timings TS1 to TS9 and timings TS10a and TS11a at which the first dispensing process 51a is executed.

  In this manner, by moving the timings TS10 and TS11 backward except for the timing TS12 of the first timing chart TC1, the timing chart TC1 of the first timing chart TC1 from the rising edge of the timing TS9 to the falling edge of the timing TS11a. A time Td2 longer than the time Td1 assigned from the rise of the timing TS9 to the fall of the timing TS11 can be assigned.

  Thereby, the interval between the fall of the timing TS9 and the rise of TS10a can be made longer than the interval between the fall of the timing TS9 and the rise of TS10 in the first timing chart TC1. Further, the interval between the fall of the timing TS10a and the rise of the TS11a can be made longer than the interval between the fall of the timing TS10 and the rise of the TS11 in the first timing chart TC1.

  In the first dispensing process 51a, step S1 is executed at timing TS1, and step S2 is executed at timing TS2. Further, step S3 is executed at timing TS3, and step S4 is executed at timing TS4. Further, step S5 is executed at timing TS5, and step S6a is executed at timing TS6. Further, step S7 is executed at timing TS7, and step S8 is executed at timing TS8. Further, step S9 is executed at timing TS9, and step S10 is executed at timing TS10a. Further, Step S11 is executed at the timing TS11a.

  In this way, by controlling the dispensing operation of the sample dispensing probe 19 according to the first dispensing process 51a and the first timing chart TC1a, the sample dispensing probe 19 is lowered to the discharge position and then the sample P2 is removed. The time until the ejection is started can be set longer than the time from when the sample dispensing probe 19 is lowered to the ejection position until the ejection of the sample P1 is started according to the first timing chart TC1.

  Thereby, in the dispensing of the sample P2 of the inspection item B in which the dispensing amount Vb of the minute amount VL or less is set, the time until the vibration of the sample dispensing probe 19 that causes a decrease in the dispensing accuracy is settled. It can be ensured and can be dispensed with high precision without reducing the processing speed.

  Further, by controlling the dispensing operation of the sample dispensing probe 19 in accordance with the first dispensing process 51a and the first timing chart TC1a, the sample dispensing probe 19 has finished discharging the sample P2 at the discharge position. The time until the rise starts can be set longer than the time from when the sample dispensing probe 19 finishes the discharge operation of the sample P1 at the discharge position to the start of the rise according to the first timing chart TC1.

  Thereby, in the dispensing of the sample P2 of the inspection item B in which the dispensing amount Vb of the minute amount VL or less is set, the sample dispensing probe 19 that causes a decrease in dispensing accuracy is dispensed with a delay in the discharge operation. It is possible to secure the time until the discharge of the amount Pb of the sample P2 is completed, and it is possible to dispense accurately without reducing the processing speed.

  It should be noted that the time when the sample dispensing probe 19 descends from the upper stop position to the discharge position using the time Td2 is the time when the sample dispensing probe 19 descends from the upper stop position to the discharge position according to the first timing chart TC1. The lowering speed may be set longer than the time when the first timing chart TC1 is taken, and the lowering speed may be lower than that in the case of the first timing chart TC1. Thereby, in the dispensing of the sample P2 of the inspection item B in which the dispensing amount Vb of the minute amount VL or less is set, the sample dispensing probe 19 that causes a decrease in dispensing accuracy is stopped at the discharge position. In order to reduce the impact, it is possible to secure a time for lowering at a low speed, and it is possible to dispense accurately without reducing the processing speed.

  Further, using the time Td2, the time when the sample dispensing probe 19 rises from the discharge position to the upper stop position is longer than that in the case of the first timing chart TC1, and the rising speed is set to the first timing chart TC1. You may implement so that it may become slower than the case of. Thereby, when dispensing the sample P2 of the inspection item D in which the dispensing amount Vd is less than or equal to the minute amount VL, when stopping at the stop position on the sample dispensing probe 19 which causes a decrease in dispensing accuracy. In order to reduce the impact, it is possible to secure a time for raising at a low speed, and it is possible to dispense accurately without reducing the processing speed.

  Furthermore, using the time Td2, the time when the sample dispensing probe 19 discharges the sample P2 at the discharge position is set longer than in the case of the first timing chart TC1, and the discharge speed of the sample P2 is set to the first timing. You may implement so that it may become slower than the case of chart TC1. Thereby, in the dispensing of the sample P2 of the inspection item B in which the dispensing amount Vb of the minute amount VL or less is set, the sample dispensing probe 19 that causes a decrease in dispensing accuracy is dispensed with a delay in the discharge operation. It becomes possible to prevent the discharge of the sample P2 of the amount Vb from being finished, and it is possible to dispense accurately without reducing the processing speed.

  FIG. 14 is a diagram illustrating a second timing chart TC2a. The second timing chart TC2a is different from the second timing chart TC2 in that the timings TS2, TS4 and the timings TS6 to TS8 are excluded, and the timings TS9, TS10 are replaced with the timings TS9b, TS10b moved forward. Is a point. As a result, the second timing chart TC2a includes “rotation” and “stop” of the reaction table 18, and timings TS9b, TS10b, TS11, and TS12 at which the second dispensing step 52a is executed.

  Thus, by moving the timings TS9 and TS10 forward except for the timings TS2 and TS4 and the timings TS6 to TS8 of the second timing chart TC2, from the rising of the timing TS9b to the falling of the timing TS11, A time Td2 longer than the time Td1 allocated from the rise of the timing TS9 to the fall of the timing TS11 in the second timing chart TC2 can be assigned.

  As a result, the interval between the fall of the timing TS9b and the rise of TS10b can be made longer than the interval between the fall of the timing TS9 and the rise of TS10 in the second timing chart TC2. Further, the interval between the fall of the timing TS10b and the rise of TS11 can be made longer than the interval between the fall of the timing TS10 and the rise of TS11 in the second timing chart TC2.

  In the second dispensing step 52a, step S9 is executed at timing TS9b, and step S10 is executed at timing TS10b. Further, step S11 is executed at timing TS11, and step S12 is executed at timing TS12.

  In step S11 of the first dispensing process 51a, the reaction table 18 of the second timing chart TC2a stops the sample dispensing probe 19 that has risen to the upper stop position of the reaction container 17 that stops at the reaction container position C10. In the meantime, for example, in the washing tank 31 in the vicinity of the upper stop position, it is moved to the upper stop position according to the stop of the reaction table 18, and step S9 of the second dispensing step 52a is executed at timing TS9b. You may carry out so that it may.

  In this way, by controlling the dispensing operation of the sample dispensing probe 19 according to the second dispensing process 52a and the second timing chart TC2a, the sample dispensing probe 19 is lowered to the discharge position and then the sample P2 is removed. The time until the ejection is started can be set longer than the time from when the sample dispensing probe 19 is lowered to the ejection position until the ejection of the sample P1 is started according to the second timing chart TC2.

  Thereby, in the dispensing of the sample P2 of the inspection item D in which the dispensing amount Vd of the minute amount VL or less is set, the time until the vibration of the sample dispensing probe 19 that causes a decrease in the dispensing accuracy is settled. It can be ensured and can be dispensed with high precision without reducing the processing speed.

  Further, by controlling the dispensing operation of the sample dispensing probe 19 according to the second dispensing process 52a and the second timing chart TC2a, the sample dispensing probe 19 finishes the dispensing operation of the sample P2 at the ejection position. The time until the rising starts can be set longer than the time from when the sample dispensing probe 19 finishes discharging the sample P1 at the discharge position until the rising starts according to the second timing chart TC2.

  As a result, in the dispensing of the sample P2 of the inspection item D in which the dispensing amount Vd of a minute amount VL or less is set, the sample dispensing probe 19 that causes a decrease in dispensing accuracy is delayed after the discharge operation. It is possible to secure a time until the discharge of the sample P2 of the amount Vd is completed, and it is possible to dispense accurately without reducing the processing speed.

  Note that the time when the sample dispensing probe 19 descends from the upper stop position to the discharge position using the time Td2 is longer than that in the second timing chart TC2, and the descending speed is set in the second timing chart TC2. You may implement so that it may become slower than the case of. Thereby, in the dispensing of the sample P2 of the inspection item D in which the dispensing amount Vd equal to or less than the minute amount VL is set, the sample dispensing probe 19 that causes a decrease in dispensing accuracy is stopped at the discharge position. In order to reduce the impact, it is possible to secure a time for lowering at a low speed, and it is possible to dispense accurately without reducing the processing speed.

  Further, when a dispensing amount Vf of a minute amount VL or less is set in the inspection item F and the inspection items B, D, and F are set in the sample P2, the dispensing amount Vb, the dispensing amount Vd, and the dispensing amount are set. When the total amount of Vf is equal to or less than the upper limit amount VM (Vb + Vd + Vf), in step S6a of the first dispensing step 51a in the first cycle, the total amount (Vb + Vd + Vf) of the sample P2 is sucked. May be. In this case, in step S10 of the first cycle, the reaction of stopping the sample P2 of the dispensing amount Vb set in the first inspection item B among the three inspection items B, D, F at the reaction container position C10. It is discharged into the container 17. In step S10 of the second dispensing step 52a in the second cycle, the sample P2 having the dispensing amount Vd set in the second inspection item D among the three inspection items B, D, and F is reacted. Discharge into the reaction vessel 17 stopped at the vessel position C10. Furthermore, in step S10 of the second dispensing step 52a in the third cycle, the sample P2 having the dispensing amount Vf set in the third inspection item F among the three inspection items B, D, F is reacted. Discharge into the reaction vessel 17 stopped at the vessel position C10. This makes it possible to secure a time that causes a drop in dispensing accuracy in dispensing the sample P2 of each inspection item B, D, and F in which a small amount VL or less is set, and the processing speed Can be dispensed with high accuracy without lowering.

  Furthermore, using the time Td2, the time when the sample dispensing probe 19 discharges the sample P2 at the discharge position is set longer than in the case of the second timing chart TC2, and the discharge speed of the sample P2 is set to the second timing. You may implement so that it may become slower than the case of chart TC2. As a result, in the dispensing of the sample P2 of the inspection item D in which the dispensing amount Vd of a minute amount VL or less is set, the sample dispensing probe 19 that causes a decrease in dispensing accuracy is delayed after the discharge operation. It becomes possible to prevent the discharge of the sample P2 of the amount Vd from being finished, and it is possible to dispense accurately without reducing the processing speed.

Next, dispensing of the sample P3 will be described.
FIG. 15 is a view showing each inspection item set on the sample P3 and a dispensing process and timing chart executed based on the inspection item. Inspection items A, B, C, D, and E are set for the sample P3, and the sample P3 is dispensed in the order of inspection item A, inspection item B, inspection item C, inspection item D, and inspection item E. And the dispensing amount Va which becomes the total amount (Va + Vb) below the upper limit amount VM is added to the first and second inspection items A and B including the inspection item B in which the dispensing amount Vb below the minute amount VL is set. And a dispensing amount Vb are set. In the third inspection item C, a dispensing amount Vc that is the upper limit amount VM is set. In addition, the fourth and fifth inspection items D and E including the inspection item D for which a small amount VL or less of the dispensing amount Vd has been set are dispensed amounts Vd that are the total amount (Vd + Ve) less than or equal to the upper limit amount VM. And a dispensing amount Ve are set.

  In this case, the analysis control unit 41 causes the sample P3 to be dispensed in the first cycle based on the first dispensing process 51a and the first timing chart TC1a. In addition, based on the second dispensing step 52a and the second timing chart TC2a in the second cycle, dispensing of the sample P3 is executed. Further, in the third cycle, based on the second dispensing step 52 and the second timing chart TC2, dispensing of the sample P3 is executed. In addition, based on the third dispensing process 53 and the third timing chart TC3, the dispensing of the sample P3 is executed in the fourth cycle. Further, in the fifth cycle, the sample P3 is dispensed based on the second dispensing step 52a and the second timing chart TC2a.

  In step S6a in the first dispensing step 51a of the first cycle in dispensing the sample P3, the sample dispensing probe 19 that has been lowered to the suction position receives a total amount (Va + Vb) of the sample P3 as shown in FIG. Suction. In step S10, the sample dispensing probe 19 that has been lowered to the discharge position uses the sample P3 of the dispensing amount Va set in the first inspection item A of the two inspection items A and B as the reaction container position. Discharge into the reaction vessel 17 stopped at C10.

  In step S9 in the second dispensing step 52a in the second cycle, the sample dispensing probe 19 that has been waiting at the upper stop position in the reaction vessel position C10 in step S11 in the first dispensing step 51a in the first cycle is Then, it descends to the discharge position in the reaction container 17 that stops at the reaction container position C10. Further, in step S10, the sample dispensing probe 19 lowered to the discharge position uses the sample P3 of the dispensing amount Vb set in the second inspection item B of the two inspection items A and B as the reaction container 17. Discharge inside.

  In step S6 in the second dispensing process 52 of the third cycle, the sample dispensing probe 19 that has been lowered to the suction position sucks the sample P3 of the dispensing amount Vc for the inspection item C. In step S10, the sample dispensing probe 19 lowered to the discharge position discharges the sample P3 of the dispensing amount Vc into the reaction container 17 at the reaction container position C10.

  In the third dispensing step 53 of the fourth cycle, the sample dispensing probe 19 sucks the total amount (Vd + Ve) of the sample P3 for the inspection items D and E, and out of the two inspection items D and E. A sample P3 having a dispensing amount Vd set in the first inspection item D is discharged into the reaction container 17.

  In the second dispensing step 52a of the fifth cycle, the sample dispensing probe 19 reacts the sample P3 having the dispensing amount Ve set in the second inspection item E of the two inspection items D and E. Discharge into the container 17.

  FIG. 17 is a flowchart showing the third dispensing process 53. The third dispensing process 53 includes steps S2, S4, S6a obtained by removing steps S1, S3, and S5 from the first dispensing process 51a, and steps S7 to S11.

  In step S6a in the third dispensing step 53 of the fourth cycle, the sample dispensing probe 19 lowered to the suction position aspirates the total amount (Vd + Ve) of the sample P3 as shown in FIG. Further, in step S10, the sample dispensing probe 19 lowered to the discharge position uses the sample P3 of the dispensing amount Vd set in the first inspection item D of the two inspection items D and E as the reaction container position. Discharge into the reaction vessel 17 stopped at C10. In step S11, the sample dispensing probe 19 that has discharged the sample P3 is raised to the upper stop position of the reaction container 17 that stops at the reaction container position C10.

  In step S9 in the second dispensing step 52a of the fifth cycle, the sample dispensing probe 19 that has been waiting at the upper stop position is lowered to the discharge position in the reaction container 17 that stops at the reaction container position C10. Further, in step S10, the sample dispensing probe 19 lowered to the discharge position uses the sample P3 of the dispensing amount Vb set in the second inspection item B of the two inspection items A and B as the reaction container 17. Discharge inside.

  FIG. 19 is a diagram illustrating a third timing chart TC3. The third timing chart TC3 is different from the first timing chart TC1a in that the timings TS1, TS3, and TS5 are excluded. As a result, the third timing chart TC3 is based on the “rotation” and “stop” of the reaction table 18, the timings TS2, TS4, the timings TS6 to TS9, and the timings TS10a, TS11a at which the third dispensing step 53 is executed. Composed.

  In the third dispensing process 53, step S2 is executed at timing TS2, and step S4 is executed at timing TS4. Further, step S6a is executed at timing TS6, and step S7 is executed at timing TS7. Further, step S8 is executed at timing TS8, and step S9 is executed at timing TS9. Further, step S10 is executed at timing TS10a, and step S11 is executed at timing TS11a.

  In this way, by controlling the dispensing operation of the sample dispensing probe 19 according to the third dispensing process 53 and the third timing chart TC3, the sample dispensing probe 19 is lowered to the discharge position and then the sample P3 is removed. The time until the ejection is started can be made longer than in the case of the first timing chart TC1.

  Thereby, in the dispensing of the sample P3 of the inspection item D in which the dispensing amount Vd of the minute amount VL or less is set, the time until the vibration of the sample dispensing probe 19 that causes a decrease in dispensing accuracy is settled. It can be ensured and can be dispensed with high precision without reducing the processing speed.

  Further, by controlling the dispensing operation of the sample dispensing probe 19 according to the third dispensing process 53 and the third timing chart TC3, the sample dispensing probe 19 has finished discharging the sample P3 at the discharge position. The time until the start of the rise can be made longer than in the case of the first timing chart TC1.

  As a result, in the dispensing of the sample P3 of the inspection item D in which the dispensing amount Vd equal to or less than the minute amount VL is set, the sample dispensing probe 19 that causes a drop in dispensing accuracy is dispensed after the discharge operation. It is possible to secure the time until the discharge of the sample P3 of the amount Vd is completed, and it is possible to dispense accurately without reducing the processing speed.

  Note that the time when the sample dispensing probe 19 descends from the upper stop position to the discharge position is set longer than that in the first timing chart TC1 using the time Td2, and the descending speed is set in the first timing chart TC1. You may implement so that it may become slower than the case of. Thereby, in the dispensing of the sample P3 of the inspection item D in which the dispensing amount Vd equal to or less than the minute amount VL is set, the sample dispensing probe 19 that causes a decrease in dispensing accuracy is stopped at the discharge position. In order to reduce the impact, it is possible to secure a time for lowering at a low speed, and it is possible to dispense accurately without reducing the processing speed.

  Further, by using the time Td2, the time when the sample dispensing probe 19 discharges the sample P2 at the discharge position is longer than that in the case of the first timing chart TC1, and the discharge speed of the sample P2 is set to the first timing. You may implement so that it may become slower than the case of chart TC1. As a result, in the dispensing of the sample P3 of the inspection item D in which the dispensing amount Vd equal to or less than the minute amount VL is set, the sample dispensing probe 19 that causes a drop in dispensing accuracy is dispensed after the discharge operation. It becomes possible to prevent the discharge of the amount Pd of the sample P3, and it is possible to dispense accurately without reducing the processing speed.

  According to the embodiment described above, the inspection item B in which the dispensing amount Vb of the minute amount VL or less is set and the inspection item D in which the dispensing amount Vd is set are set in the sample P2, and the two inspection items B are set. , D is the total amount (Vb + Vd) in which the sum of the dispensed amount Vb and the dispensed amount Vd is equal to or less than the upper limit amount VM, the first and second dispensing steps 51a, 52a and the first and second Based on the timing charts TC1a and TC2a of No. 2, dispensing of the sample P2 of each inspection item B and D can be executed.

  Further, two inspection items A and B including the inspection item B in which a dispensing amount Vb of a minute amount VL or less is set are set in the sample P3, and the dispensing amount Va set in the two inspection items A and B is set. And the dispensing amount Vb is a total amount (Va + Vb) equal to or less than the upper limit amount VM, based on the first and second dispensing steps 51a and 52a and the first and second timing charts TC1a and TC2a. The sample dispensing probe 19 can be made to dispense the sample P3 of the inspection item B.

  Further, two inspection items D and E including the inspection item D in which a dispensing amount Vd equal to or less than a minute amount VL is set are set in the sample P3, and the dispensing amount Vd set in the two inspection items D and E is set. And the dispensing amount Ve is a total amount (Vd + Ve) equal to or less than the upper limit amount VM, the dispensing of the sample P3 of the inspection item D is performed based on the third dispensing step 53 and the third timing chart TC3. Can be executed.

  As described above, in dispensing a small amount of sample, it is possible to secure a time that causes a drop in dispensing accuracy, and it is possible to dispense accurately without reducing the processing speed.

  Although several embodiments of the present invention have been described, these embodiments are presented by way of example and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, replacements, and changes can be made without departing from the scope of the invention. These embodiments and modifications thereof are included in the scope and gist of the invention, and are included in the invention described in the claims and the equivalents thereof.

DESCRIPTION OF SYMBOLS 11 Sample container 17 Reaction container 19 Sample dispensing probe 41 Analysis control part 100 Automatic analyzer

Claims (10)

In an automatic analyzer that dispenses a sample and a reagent into a reaction container and measures a mixed solution of the sample and the reagent in the reaction container,
A dispensing probe for aspirating the sample in the sample container and performing dispensing to discharge the sample into the reaction container in such an amount that the dispensing amount set in the inspection item is less than or equal to the upper limit amount for each inspection item; ,
A plurality of inspection items including an inspection item in which a dispensing amount equal to or less than a predetermined amount smaller than the upper limit amount is set in the sample, and a dispensing amount equal to or less than the predetermined amount is set among the plurality of inspection items. And an analysis control unit that causes the dispensing probe to aspirate the total amount of the sample in which the total amount of dispensing set to the two or more inspection items including the inspection item is equal to or less than the upper limit amount. A featured automatic analyzer.   The automatic analyzer according to claim 1, wherein the upper limit amount is a maximum amount that the sample dispensing probe can discharge into one reaction container.   The analysis control unit, when only the inspection item in which a dispensing amount exceeding the predetermined amount is set is set in the sample, causes the sample to be aspirated with a dispensing amount exceeding the predetermined amount. The automatic analyzer according to claim 1 or 2. The reaction vessel moves for each cycle and stops at a position different from that before the movement,
The dispensing probe aspirates the sample in the total amount during one cycle and then reacts the sample in a dispensing amount set as a first inspection item of the two or more inspection items. The second inspection of the two or more inspection items is discharged into the reaction container stopped at the container position, and stopped at the reaction container position during the next cycle of the one cycle. The automatic analyzer according to any one of claims 1 to 3, wherein the sample in a dispensing amount set in the item is discharged.   The dispensing probe discharges the sample of the dispensing amount set in the first inspection item until the reaction container stops at the reaction container position during the next cycle of the one cycle. 5. The automatic analyzer according to claim 4, wherein the apparatus waits at or near the upper stop position above the reaction container that stops at the reaction container position. The dispensing probe descends from an upper stop position above the reaction vessel to a discharge position where the sample can be discharged,
The time from when the dispensing probe descends to the discharge position until the dispensing of the sample with the dispensing amount set in the first inspection item or the second inspection item is started is as follows. 6. The method according to claim 4, wherein a time period from when the dispensing probe that sucks the sample in a dispensing amount exceeding a fixed amount is lowered to the ejection position to when ejection of the sample is started is longer. Automatic analyzer described in 1. The dispensing probe descends from an upper stop position above the reaction container to a discharge position where the sample can be discharged, and then rises to the upper stop position after discharging the sample at the discharge position,
The time from when the dispensing probe finishes the dispensing operation of the dispensing amount set in the first or second inspection item at the ejection position until it starts to rise is the predetermined amount. It is longer than the time from when the dispensing probe that has aspirated the sample with a dispensing amount exceeding the end of the dispensing operation of the sample with the dispensing amount at the discharge position until the sample starts to rise. The automatic analyzer according to claim 4 or 5. The dispensing probe descends from an upper stop position above the reaction vessel to a discharge position where the sample can be discharged,
The speed at which the dispensing probe that discharges the sample of the dispensing amount set in the first inspection item or the second inspection item descends from the upper stop position to the discharge position is 6. The method according to claim 4, wherein the dispensing probe that discharges the sample in a dispensing amount exceeding a predetermined amount is slower than a speed at which the dispensing probe descends from the upper stop position to the ejection position. The automatic analyzer described. The dispensing probe descends from an upper stop position above the reaction container to a discharge position where the sample can be discharged, and then rises to the upper stop position after discharging the sample at the discharge position,
The speed at which the dispensing probe that ejects the sample of the dispensing amount set in the first inspection item or the second inspection item rises from the ejection position to the upper stop position is 6. The method according to claim 4, wherein the dispensing probe that has ejected the sample in a dispensing amount exceeding a predetermined amount is slower than a speed at which the dispensing probe rises from the ejection position to the upper stop position. The automatic analyzer described. The dispensing probe descends from an upper stop position above the reaction container to a discharge position where the sample can be discharged, and then rises to the upper stop position after discharging the sample at the discharge position,
The time when the dispensing probe discharges the sample in the dispensing amount set in the first inspection item or the second inspection item at the discharge position is the time at which the dispensing probe is at the discharge position. 6. The automatic analyzer according to claim 4, wherein the time is longer than a time required for discharging the sample in a dispensing amount exceeding the predetermined amount.

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