JP2016064993A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel functional material for cosmetics consisting of a naturally occurring component excellent in a biosafety and efficacy.SOLUTION: The invention provides a cosmetic containing, as an active ingredient, an extract obtained from the flower part of the plants belonging to Rosaceae Fragaria, and having an anti-oxidant effect, gelatinase activity inhibitory effect, elastase activity inhibitory effect, and protein saccharification inhibitory effect. The cosmetic has the preventive and/or improving effect on rough and dry skin (inflammation, dry skin, acne, etc.) and aging phenomena (wrinkle, sagging, decreased suppleness or texture quality, etc.), as well as the preventive and/or improving effect on pigmentation, such as stains, freckles, and chloasma on the skin, and additionally the effect of protecting the hair and of improving the health of the scalp.SELECTED DRAWING: None

Description

  The present invention relates to a cosmetic compounding ingredient obtained from a plant belonging to the genus Rosaceae belonging to the family Rosaceae and having excellent skin physiological activity and biosafety, and a skin care cosmetic for whitening and anti-aging comprising such an ingredient, and It relates to cosmetics for hair care.

  In the skin, internal factors such as inactivation of cell growth / differentiation with age, decrease in hormone secretion, quantitative decrease in extracellular matrix components, active oxygen induced by sunlight (ultraviolet rays), air pollutants And chemical substances such as environmental hormones, allergens such as pollen, and external factors such as environmental stress are intricately intertwined, resulting in aging, rough skin, and changes in color.

  For example, various enzymes called matrix metaproteases exist in skin cells, and when activated, the basement membrane components (collagen, laminin, etc.) constituting the skin, and elastin, which is a dermal matrix component, are decomposed. As a result, it causes skin wrinkles and tarmi.

  In addition, external factors such as ultraviolet rays, chemical substances, and allergens damage skin cells, tissues, and cells to alter biological components, resulting in the generation of antigens in the skin, thereby Inflammation occurs. Furthermore, it induces abnormal deposition of melanin pigments, causing spots, freckles, and liver spots.

  In addition, glycation reaction of skin protein is known as a skin aging phenomenon, and accumulation of protein glycation end product (advanced glycation end product (AGE)) generated by the reaction attracts attention. Collecting. When this protein saccharification reaction occurs in the skin, the function of skin proteins (collagen, elastin, etc.) is impaired, and as a result, skin flexibility and elasticity are lost, causing wrinkles and tarmi. Moreover, when AGE accumulates in the skin, it may cause spots and dullness.

  In order to prevent aging and unhealthy skin as described above, and to maintain a youthful state, the use of various active ingredients has been proposed in the past, and these active ingredients (beautifying agents, whitening agents, etc.) have been proposed. Blended cosmetics are on the market. For example, antioxidants such as vitamin C, vitamin E and catalase; anti-inflammatory agents such as glycyrrhizic acid; various ultraviolet absorbers; cell activation such as α-hydroxycarboxylic acid, placenta extract, γ-amino-β-hydroxybutyric acid Ingredients: extracellular matrix components such as collagen, elastin and hyaluronic acid; humectants such as urea and protein saccharification inhibitors such as aminoguanidine. Further, arbutin, kojic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots, buckwheat, and melasma, and are widely used as active ingredients for whitening agents.

  As described above, anti-aging agents and whitening agents have been proposed based on various mechanisms of skin aging phenomenon. However, the safety of the skin and the effectiveness when actually applied to the skin has been proposed. There is a problem in terms of perspective. Accordingly, there is a need for cosmetic ingredients with improved such points.

  In view of the problems of the prior art, the present inventors have conducted intensive research to find a new cosmetic compounding ingredient derived from a natural product from the viewpoint of skin safety. As a result, the flower part extract of the plant belonging to the genus Rosaceae belonging to the family Rosaceae has excellent antioxidant action, gelatinase activity inhibitory action, elastase activity inhibitory action and protein saccharification inhibitory action. By blending the extract, it is possible to provide cosmetics that have excellent skin safety (including the scalp), anti-aging effect, anti-aging effect, whitening effect, hair quality improvement and hair growth effect. I found out that

  Conventionally, the use of extracts of seeds, fruits, leaves, and stems of plants belonging to the genus Rosaceae of the Rosaceae family for skin external preparations such as cosmetics has been publicized by the description of Patent Documents 1 to 4 and the like. However, it has not been known about using the flower extract of the plant as a cosmetic.

Japanese Patent Laid-Open No. 2003-261454 JP 2008-273922 A JP 2009-298765 JP JP 2010-047483 A

The present invention is a cosmetic containing, as an active ingredient, an extract of the flower part of a plant belonging to the genus Rosaceae of the Rosaceae family.
In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

  The present invention is a cosmetic comprising, as an active ingredient, an extract of a flower part of a plant belonging to the genus Rosaceae of the Rosaceae family, and has an antioxidant action (SOD-like activity, lipid peroxide suppression), gelatinase activity exhibited by the extract. Inhibiting action, elastase activity inhibiting action and protein glycation inhibiting action, prevent and improve skin roughness (inflammation, dry skin, acne, etc.) and aging phenomena (decrease in wrinkles, tarmi, tension and texture, etc.) In addition, it is possible to provide a cosmetic that prevents and improves pigmentation such as skin spots, buckwheat and melasma. Furthermore, the cosmetics which have the effect of protecting hair based on the said effect | action and making the scalp healthy can also be provided.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The extraction material used in the present invention is preferably the use of the flower part of the Rosaceae (Frosaria) or the whole plant including the flower part, or the flower part and the stem and / or leaf. Plants of the Dutch strawberry genus include: strawberry, chili strawberry, carpenter strawberry, white snake strawberry, Ezo snake strawberry, exotic strawberry, Virginia strawberry, Fragaria grandiflora, Fragaria daltoniana, Fragaria nilgerrensis, Fragaria nubicola, Fragaria viridis, Fragaria moupinensch, Fragararia orientalis , Fragaria iturupensis Staudt, or a variant or hybrid of these can also be used. Moreover, the collection time and size of the flower are not particularly limited, and any size and collection time may be used. In addition, in this invention, a flower part is a thing of the state of a bud before flowering, or a thing containing any one or more of the petal after flowering, a bud, a stamen, a pistil, and a flower bed (the thing of an immature stage). good.

  The extract is prepared by first washing the strawberry part (eg, flower part, whole grass, or flower part and stem and / or leaf) of the Rosaceae family with water if necessary to remove foreign substances. Then, as it is or after drying, it is minced or pulverized as necessary, and extracted by contacting with an extraction solvent. The extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but a supercritical extraction method or a steam distillation method can also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, water and lower alcohols are used in the present invention from the viewpoint of the effectiveness of the extract obtained, further from the viewpoint of skin irritation, and from the viewpoint that it can be widely applied to cosmetics. Or hydrophilic solvents such as polyhydric alcohols are preferred. Preferable examples of using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols. Examples include the use of a mixed solvent with (especially ethanol) or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin). Among them, water alone or water with 1,3 -A mixed solvent of butylene glycol is particularly preferred.

  When the mixed solvent is used, for example, if the mixed solvent is water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1: 1 to 20: 1, and the mixed solvent is water and ethanol. If it exists, it is preferable to set it as the range of 1: 1 to 20: 1 if it is 1: 1 to 25: 1 and the mixed solvent of water and glycerol.

  Moreover, the weight ratio of the dried part (for example, a flower part, the whole plant including a flower part, or a flower part, a stem, and / or a leaf) and the extraction solvent of a rose genus Strawberry is preferably 1: 1-1. : 80, and more preferably 1: 5 to 1:50.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 4-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. The extraction temperature is preferably in the range of 0 ° C to 80 ° C, more preferably in the range of 0 ° C to 20 ° C, and the extraction time is preferably 1 hour to 7 days, more preferably 4 hours. ~ 3 days range.

  In addition, you may perform a hydrolysis process to a strawberry as needed prior to the extraction process of this invention, or in parallel with an extraction process. Thereby, the storage stability of the extract may be improved, and the extract as a cosmetic compounding agent may be used more effectively.

  In the case of subjecting the extract to an enzymatic hydrolysis treatment, examples of the enzyme include proteolytic enzymes such as actinase, papain and pepsin, starch degrading enzymes such as glucoamylase, α-amylase and β-amylase, cellulase, hemicellulase and pectinase. One or two or more selected from the group of enzymes of lipolytic enzymes such as fibrinolytic enzymes and lipases may be used, but one or more selected from these enzyme groups, respectively. It is more preferable to use a combination of these enzymes.

  The amount of the enzyme added is, for example, 0 in total with respect to the solid content of the flower part, the whole plant including the flower part, or the flower part and the stem and / or leaf belonging to the genus Rosaceae of the Rosaceae family. The range is preferably 0.01 to 50% by weight, more preferably 0.1 to 10% by weight.

  In general, the extract prepared as described above may be adjusted to a pH of 4 to 9 and used as it is as a cosmetic compounding agent as it is or as a desired concentration by vacuum concentration or the like. You may do it. In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the extract prepared as described above may be refrigerated for a certain period of time in order to enhance storage stability and the like, and the supernatant may be used as a cosmetic compounding agent.

  Cosmetics (including quasi drugs) containing the strawberry extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, hoho Examples include makeup cosmetics such as red and white powder, cleansing cosmetics such as facial cleansers, body shampoos and soaps, hair cosmetics such as shampoos, hair conditioners and hair creams, and bath preparations. It is not limited to.

  The amount of the extract of the plant belonging to the genus Rosaceae belonging to the family Rosaceae in the cosmetics of the present invention is generally 0.002 to 1.0% by weight, preferably 0, in the case of basic cosmetics, as the solid content of the extract. In the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. The case is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0 wt% (solid content wt%, the same shall apply hereinafter), preferably 0.0001 to 3 0.0% by weight.

  For cosmetics, in addition to the plant extract belonging to the Dutch strawberry genus, which is an essential ingredient, ingredients commonly used in cosmetics, such as oily ingredients, surfactants (synthetic and natural products), moisturizers, thickeners Antiseptic / bactericidal agents, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances, and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics of the extract are not impaired, there may be no combination of other physiologically active ingredients.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, isopropyl palmitate, oleic acid Butyl, - ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers and emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, and soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; silane root (white) extract; pectin, locust bean gum, aloe polysaccharides, alkaligenes-producing polysaccharides, etc. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Ethanol derived from plants such as Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry product, radish fermentation liquor, sugarcane, etc. Examples include 3-butylene glycol.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , Adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyltocopherylmaleic acid, ascorbyltocopherylphosphate K, myristyl 3-glyceryl ascorbi Acids, ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, acylated 6-positions of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or Acylated derivatives thereof, glycerin derivatives of ascorbic acid such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl Examples of hydroquinone derivatives include scorbyl phosphate, arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives such as tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propyl. Examples of nicotinic acid derivatives include nicotinic acid amide and benzyl nicotinate, and α-hydroxy acids include, for example, lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract or hydrolyzate thereof, pearl hydrolyzate, pearl seed fermented product, Royal jelly fermented product, sake lees extract or ceramide contained therein, sake lees fermented product, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. It is done. Also, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe Extracts of seaweeds such as seaweed, extracts of marine flowering plants such as sea cucumber, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermentation extract, germinated rice extract or hydrolyzate thereof, germinated rice Fermented product, black bean extract or hydrolyzate thereof, damask rose flower extract, bamboo shoot peel extract, linoleic acid and its derivatives or processed products (such as liposomal linoleic acid), animal or fish collagen And derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, dii Propylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, aloe extract, honey comb extract, loofah extract, anaosa extract, peach extract, peach extract, Kiwi extract, sunflower extract, Zizyphus joazeiro extract, Paudarco bark extract, dairy lily extract or fermented product, Hibiscus flower extract or fermented product, Hagoromogusa extract, Cherimoya extract, mango extract , Mangosteen extract, funori extract, oolong tea extract, red rich extract, purple orchid extract, yam peel or seed coat extract or hydrolysate, safflower flower extract, casablanca extract, sweet potato extract or fermented product , Guava leaf extract, dokudami extract, evening birch extract, aloe extract, apple extract And the like.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of strawberry flower part extract Dry strawberry flower part, add 315 g of purified water and 135 g of 1,3-butylene glycol to 15 g of dried product, soak at 4 ° C., extract, filter, 338 g of a brown transparent strawberry flower extract was obtained (solid content concentration 1.5%). In addition, when using the extract which concerns on this manufacture example 1 for the evaluation test mentioned later, it prepared so that the solid content concentration might be 0.2%.

Production Example 2 Preparation of strawberry flower part extract Dry strawberry flower part (including some stems), add 315 g of purified water and 135 g of 1,3-butylene glycol to 15 g of dried product, and soak at 4 ° C for extraction. After filtration, 0.75 g of activated carbon is added to 338 g of the obtained liquid, and the mixture is stirred for 1 hour. The activated carbon was removed by filtration to obtain 272 g of a light brown transparent strawberry flower extract (solid content concentration 1.0%). In addition, when using the extract which concerns on this manufacture example 2 for the evaluation test mentioned later, it prepared so that the solid content concentration might be 0.2%.

Production Example 3 Preparation of strawberry flower part extract Strawberry flower part (strawberry) was dried, 315 g of purified water, 135 g of 1,3-butylene glycol and 0.9 g of lactic acid were added to 15 g of dried product, and immersed at 4 ° C. After extraction, the mixture is filtered, and 0.75 g of activated carbon is added to 335 g of the obtained liquid, followed by stirring for 1 hour. The activated carbon was removed by filtration to obtain 270 g of a light brown transparent strawberry flower extract (solid content concentration 1.0%). In addition, when using the extract which concerns on this manufacture example 3 for the evaluation test mentioned later, it prepared so that the solid content concentration might be 0.2%.

Formulation Example 1 Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts [Component C]
Perfume Appropriate A component and B component were each heated to 80 ° C. or higher, then B component was added to A component and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 2 was used instead of the extract of Production Example 1 contained in the component B of Formulation Example 1.

Formulation Example 3 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 3 was used instead of the extract of Production Example 1 contained in the component B of Formulation Example 1.

Formulation Example 4 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount of 100 parts [component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 5 Emulsion Emulsion in the same manner as in Formulation Example 4 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. Got.

Formulation Example 6 Emulsion In the B component of Formulation Example 4, an emulsion was prepared in the same manner as Formulation Example 4 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Formulation Example 7 Emulsion In the B component of Formulation Example 4, with the exception of using 2.0 parts of rice bran extract hydrolyzate instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, An emulsion was obtained in the same manner.

Formulation Example 8 Emulsion In the same manner as in Formulation Example 4, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 9 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 10 Lotion [Ingredient] part Extract of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts.

Formulation Example 11 Lotion A lotion was obtained in the same manner as in Formulation Example 9, except that 10.0 parts of the extract of Production Example 3 was used instead of the extract of Production Example 2 in the ingredients of Formulation Example 10.

Formulation Example 12. Essence [ingredient] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Extract of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water in an amount of 100 parts Hyaluronic acid was dissolved in purified water, and then the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Example 13 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 14. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Extract of Production Example 2 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 15. Hair growth cosmetics [ingredients] Part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-Menthol 0.8
Japanese cypress raft root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Extract solution of Production Example 1 3.5
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine appropriate amount ethanol 20
The amount in which the total amount of purified water is 100 parts The above ingredients were sufficiently stirred and mixed to obtain a hair restorer.

Formulation Example 16. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Total amount of 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, stirring is continued and cooled to room temperature to obtain a hair shampoo It was.

Example 17. Hair conditioner [Component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[Component B] Part Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Purified water in an amount of 100 parts A component and B component were each heated to 80 ° C. and dissolved uniformly, then B component was added to A component, and stirring was continued to cool to room temperature to obtain a hair rinse. .

Test Example 1 DPPH radical scavenging effect evaluation test First, 2.4 parts of DPPH (1,1-diphenyl-2-picrylhydrazyl) was dissolved in 20 parts of ethanol, and 20 parts of purified water was added thereto to prepare a DPPH solution. . To 24 parts of this DPPH solution, 19.2 parts of 18 v / v% ethanol solution and 4.8 parts of 2M acetic acid-sodium acetate buffer (pH 5.5) were added to prepare a DPPH-added solution. In addition, in order to subtract the effect of the color of the extract itself on the test, a 50 v / v% ethanol solution was used instead of the DPPH solution, and a solution obtained by mixing an 18 v / v% ethanol solution and a 2M acetic acid-sodium acetate buffer solution was used. A control solution was used. Next, the extract solution of Production Example 1 prepared as described above was diluted with purified water to prepare a sample solution. Here, the sample solution was prepared such that the final concentration (concentration as a solution) of the extract solution of Production Example 1 was 0.5%, 1.0%, and 2.0%, respectively, with respect to the total amount 3 The seed concentration was used. The sample solution and DPPH added solution or control solution were mixed at a ratio of 1: 3, allowed to stand at room temperature for 10 minutes, and the absorbance at 550 nm when each test solution was mixed with DPPH added solution. The difference in absorbance at 550 nm when this was mixed with the control solution was measured to confirm the residual amount of DPPH radicals. At the same time, as a control, instead of the extract solution of Production Example 1, a 30% 1,3-butylene glycol aqueous solution was used, and the same operation as described above was performed. The relative value of the DPPH radical residual rate was determined. In addition, in order to confirm whether the test system is functioning normally, the same test was performed when water-soluble vitamin E (final concentration 10 μM) was added as a positive control instead of the sample solution.

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, it was confirmed that the extract according to Production Example 1 of the present invention has an excellent DPPH radical scavenging action in a concentration-dependent manner.

Test Example 2 Evaluation test of SOD-like activity [Test method]
First, 0.1M mL of 1M Tris-HCl buffer solution, 0.30 mL of 1 mM ethylenediaminetetraacetic acid / disodium salt solution, 0.30 mL of 1 mM xanthine solution, 0.20 mL of 0.75 mM nitrobull-tetrazolium solution, extract solution of Production Example 1 A test solution was prepared by mixing 0.10 mL and 1.90 mL of purified water. Moreover, in the test solution, a mixed solution (control) having the same composition as the above test solution was prepared except that 0.10 mL of 30% 1,3-butylene glycol was used instead of 0.10 mL of the extract of Production Example 1. In addition, a mixed solution having the same composition as the above test solution except that 0.10 mL of 0.875 or 8.75 Unit / mL superoxide dismutase solution is used instead of 0.10 mL of the extract solution of Production Example 1 in the test solution. (Positive control) was prepared. After incubating the above test solution or the sample-free mixed solution at 37 ° C., respectively, 0.05 mL of 1 Unit / mL xanthine oxidase solution was added thereto, and after a certain period of time (5 minutes), each test solution at 570 nm Absorbance (an index of the amount of superoxide anion in the test solution) was measured. As a result, the absorbance of each test solution or the mixture of the positive control (superoxide dismutase) when the absorbance of the control mixture was taken as 100% was expressed in%.

The results of Test Example 2 are shown in Table 2.
[Table 2]

  As shown in Table 2, it was confirmed that the extract according to Production Example 1 of the present invention has significantly superior SOD-like activity in a concentration-dependent manner.

Test Example 3 Evaluation test of lipid peroxide inhibitory effect First, 1.0 mL of 0.5 M ethanol linoleate, 10 mL 0.2 M phosphate buffer (pH 7.0) and 9.0 mL ethanol were mixed and sufficiently stirred. To this mixed solution, 5.0 mL of the extract solution of Production Example 1 was added and stirred sufficiently to prepare a sample solution. Here, the sample solution was prepared so that the final concentration (concentration as a solution) of the extract solution of Production Example 1 with respect to the total amount was 1.0%, 2.0%, and 5.0%, respectively. The seed concentration was used. Immediately after the preparation of this sample solution, 0.1 mL, 4.7% of 75% ethanol, 0.1 mL of 30% ammonium thiocyanate solution, and 0% were left for 1 day, 4 days, and 7 days at 40 ° C. After adding 0.1 mL of a 3.5% hydrochloric acid solution of 0.02M ferrous chloride and thoroughly mixing and stirring, the absorbance at a wavelength of 500 nm after 3 minutes was measured. Also. As a control, a similar test was performed on a 30% aqueous solution of 1,3-butylene glycol, and the amount of lipid peroxide produced in this test system was expressed as an increase in absorbance at a wavelength of 500 nm after subtracting the value immediately after preparation.

The results of Test Example 3 are shown in Table 3.
[Table 3]

  As shown in Table 3, it was confirmed that the extract according to Production Example 1 of the present invention has a markedly excellent lipid peroxide suppressing effect in a concentration-dependent manner.

Test Example 4 Evaluation Test of Gelatinase Activity Inhibitory Effect First, a culture supernatant of fibroblasts (NB1RGB) in which MMP synthesis was induced using 0.25 ng / mL of IL-1α was used as a gelatinase enzyme solution. 5 μg / mL trypsin was added to the gelatinase enzyme solution and the mixture was reacted at 37 ° C. for 30 minutes for activation treatment. The solution after stopping the reaction with 50 μg / mL trypsin inhibitor was used as a gelatinase-activating enzyme solution. After adding 120 μL of gelatinase-activating enzyme solution and 10 μL of the prepared solution of Preparation Example 1 to a final concentration of 5.0% (as a solution) to a 96-well microplate, 1 μg / mL substrate solution MOCAc− 20 μL of pro-leu-gly-leu-a2pr (DNP) -ala-arg-NH ₂ , manufactured by Peptide Institute) was added, and the initial fluorescence intensity (Ex = 355 nm, Em = 460 nm) was measured for 30 minutes. The reaction was performed at room temperature. The increase amount obtained by subtracting the initial value from the fluorescence intensity after 30 minutes was determined. The same operation was performed for the group (control) to which purified water was added instead of the extract of Production Example 1, and the relative value of the increase in fluorescence intensity relative to this control was defined as the gelatinase activity rate (%). Moreover, in order to confirm whether the test system is functioning normally, the same test was performed when 1 mM EDTA was added as a positive control instead of the sample solution.

The results of Test Example 4 are shown in Table 4.
[Table 4]

  As shown in Table 4, it was confirmed that the extract according to Production Example 1 of the present invention has an excellent gelatinase activity inhibitory effect.

Test Example 5. Evaluation Test for Elastase Activity Inhibitory Effect First, human dermis-derived fibroblasts NB1RGB are seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 10% NCS-containing Eagle's minimum essential medium, 37 ° C., 5.0 Pre-cultured for 1 day under the condition of% CO ₂ . Then, after washing once with PBS (−), 50 μL / well of 100% Tris-HCl buffer solution (pH 8.0) containing 1% Triton X-100 / 1 mM PMSF was added and allowed to stand at room temperature for 10 minutes. The cells were lysed. The solution was used as a fibroblast-derived elastase enzyme solution. 50 μL of the evaluation sample solution containing the extract solution of Production Example 1 and 50 μL of the synthetic substrate solution (5 mM Suc Ala-Ala-Ala pNA / 100 mM Tris-HCl buffer (pH 8.0)) are mixed and reacted at 37 ° C. for 2 hours. Then, the absorbance (wavelength 405 nm: microplate reader (Model 680, manufactured by Bio-Rad)) was measured and used as an index of elastase activity of fibroblasts. When the same test was performed using purified water instead of the extract solution of Production Example 1 and the fibroblast elastase activity obtained here was set to 100, the extract solution according to Production Example 1 was used. The ratio of elastase activity of the fibroblasts was determined and used as the relative fibroblast elastase activity. Moreover, in order to confirm whether the test system is functioning normally, the same test was performed when EDTA (final concentration 1 mM) was added as a positive control instead of the sample solution.

The results of Test Example 5 are shown in Table 5.
[Table 5]

  As shown in Table 5, it was confirmed that the extract according to Production Example 1 of the present invention has an excellent elastase activity inhibitory effect in a concentration-dependent manner.

Test Example 6. Evaluation Test of Protein Saccharification Inhibition Effect In this test, the generation suppression effect of AGE, that is, the protein saccharification suppression effect was evaluated by the generation of BSA (bovine serum albumin) fluorescence via glucose. Next, 40 μL of the preparation solution, 40 μL of 50 mg / mL BSA aqueous solution, 40 μL of 2.5 M glucose aqueous solution, and 80 μL of PBS (−) solution were mixed and stirred to prepare a sample solution. The sample solution was prepared so that the final concentrations of the extract solution of Production Example 1 were 1.0%, 2.0%, and 5.0%, respectively. Next, the sample solution is allowed to stand at 50 ° C. for 7 days, and after 7 days, for each sample solution, fluorescence generation (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader (Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)) Was measured. Moreover, it replaced with the extract of manufacture example 1, and performed the same operation about the case of sample addition (Control) using purified water, and relative value of the fluorescence measurement value of each sample solution with respect to the fluorescence measurement value obtained here (%) Was determined and used as the protein saccharification rate (%). Furthermore, the same test was performed when 1 mM aminoguanidine (AG) was added as a positive control instead of the extract of Production Example 1.

The results of Test Example 6 are shown in Table 6.
[Table 6]

  As shown in Table 6, it was confirmed that the extract according to Production Example 1 of the present invention has an excellent protein saccharification inhibitory effect in a concentration-dependent manner.

  Due to the above effects, the extract according to the present invention is combined to improve skin inflammation, acne, etc., and protect the skin from oxidative damage caused by allergens such as ultraviolet rays, air pollutants and pollen. Cosmetics can be provided. In addition, prevention and improvement of skin aging (wrinkles, tarmi, dullness, sharpness and gloss, reduction in texture, etc.), prevention and improvement of pigmentation such as skin spots, buckwheat, liver spots, dull pores, darkening Realized cosmetics can be provided. Furthermore, hair cosmetics that prevent dandruff, itching and aging of the scalp can be provided.

Claims (1)

Cosmetics containing, as an active ingredient, an extract of the flower part of a plant belonging to the genus Rosaceae (Fragaria).