JP2015529451A5 - - Google Patents

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JP2015529451A5
JP2015529451A5 JP2015522172A JP2015522172A JP2015529451A5 JP 2015529451 A5 JP2015529451 A5 JP 2015529451A5 JP 2015522172 A JP2015522172 A JP 2015522172A JP 2015522172 A JP2015522172 A JP 2015522172A JP 2015529451 A5 JP2015529451 A5 JP 2015529451A5
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amino acid
helicase
construct
seq
polynucleotide
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JP2015522172A
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JP2015529451A (ja
JP6429773B2 (ja
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Priority claimed from PCT/GB2013/051928 external-priority patent/WO2014013262A1/en
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JP2015522172A 2012-07-19 2013-07-18 酵素構築物 Active JP6429773B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261673446P 2012-07-19 2012-07-19
US61/673,446 2012-07-19
US201361774694P 2013-03-08 2013-03-08
US61/774,694 2013-03-08
PCT/GB2013/051928 WO2014013262A1 (en) 2012-07-19 2013-07-18 Enzyme construct

Related Child Applications (1)

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JP2018146743A Division JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

Publications (3)

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JP2015529451A JP2015529451A (ja) 2015-10-08
JP2015529451A5 true JP2015529451A5 (https=) 2016-09-01
JP6429773B2 JP6429773B2 (ja) 2018-11-28

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JP2015522172A Active JP6429773B2 (ja) 2012-07-19 2013-07-18 酵素構築物
JP2018146743A Pending JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

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US (2) US9797009B2 (https=)
EP (1) EP2875152B1 (https=)
JP (2) JP6429773B2 (https=)
AU (1) AU2013291765C1 (https=)
BR (1) BR112015001052B8 (https=)
CA (1) CA2879355C (https=)
WO (1) WO2014013262A1 (https=)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
KR20130049671A (ko) 2011-11-04 2013-05-14 한미사이언스 주식회사 생리활성 폴리펩타이드 결합체 제조 방법
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
BR112014020211A2 (pt) 2012-02-16 2017-07-04 Oxford Nanopore Tech Ltd métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico
AR090281A1 (es) 2012-03-08 2014-10-29 Hanmi Science Co Ltd Proceso mejorado para la preparacion de un complejo polipeptidico fisiologicamente activo
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2917366B1 (en) 2012-11-06 2017-08-02 Oxford Nanopore Technologies Limited Quadruplex method
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN118256603A (zh) 2013-10-18 2024-06-28 牛津纳米孔科技公开有限公司 经修饰的酶
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
AU2015242657B2 (en) * 2014-03-31 2020-05-21 Hanmi Pharm. Co., Ltd. Method for improving solubility of protein and peptide by using immunoglobulin Fc fragment linkage
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
KR102457147B1 (ko) 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
KR102837999B1 (ko) 2015-12-08 2025-07-24 카트호리이케 유니버시타이트 로이펜 변형된 나노세공, 그를 포함하는 조성물, 및 그의 용도
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP4063521B1 (en) 2016-05-25 2024-02-21 Oxford Nanopore Technologies PLC Method of nanopore sequencing
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
WO2019006214A1 (en) 2017-06-29 2019-01-03 President And Fellows Of Harvard College DETERMINISTIC PROGRESSION OF POLYMERS THROUGH A NANOPORE
WO2019002893A1 (en) 2017-06-30 2019-01-03 Vib Vzw NEW PROTEIN PORES
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569977A (en) 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
GB201808556D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201808554D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
WO2020025909A1 (en) 2018-07-30 2020-02-06 Oxford University Innovation Limited Assemblies
JP7324835B2 (ja) 2018-08-28 2023-08-10 エフ. ホフマン-ラ ロシュ アーゲー ルテニウム含有電極を備えるナノポアシーケンシングデバイス
JP7153140B2 (ja) 2018-12-19 2022-10-13 エフ.ホフマン-ラ ロシュ アーゲー 3’保護ヌクレオチド
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
US11789006B2 (en) 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
GB202112235D0 (en) 2021-08-26 2021-10-13 Oxford Nanopore Tech Ltd Nanopore
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
EP4519453A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024033443A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
KR20250096716A (ko) 2022-10-28 2025-06-27 옥스포드 나노포어 테크놀로지즈 피엘씨 포어 단량체 및 포어
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
CN119060983A (zh) * 2023-06-02 2024-12-03 北京齐碳科技有限公司 解旋酶突变体及其应用
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407100D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407099D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
GB202413506D0 (en) 2024-09-13 2024-10-30 Oxford Nanopore Tech Plc Pore monomers and their uses
GB202413618D0 (en) 2024-09-16 2024-10-30 Oxford Nanopore Tech Plc peptide characterisation
GB2644084A (en) 2024-09-16 2026-03-18 Oxford Nanopore Tech Plc Augmented consensus and variant calling
GB202413747D0 (en) 2024-09-18 2024-10-30 Oxford Nanopore Tech Plc Preprocessing nanopore signals

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602979B1 (en) 1994-12-19 2003-08-05 The United States Of America As Represented By The Department Of Health And Human Services Screening assays for compounds that cause apoptosis
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
JPWO2002092821A1 (ja) * 2001-05-01 2004-09-02 独立行政法人産業技術総合研究所 新規マキシザイム
JP4632788B2 (ja) * 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
JP4757804B2 (ja) 2004-10-27 2011-08-24 株式会社カネカ 新規カルボニル還元酵素、その遺伝子、およびその利用法
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
CA3092369C (en) 2008-09-22 2025-09-23 University Of Washington MSP NANOPORES AND ASSOCIATED PROCESSES
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
JP5861223B2 (ja) 2009-02-23 2016-02-16 サイトムエックス セラピューティクス, インク.CytomX Therapeutics, Inc. プロタンパク質およびその使用方法
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
BRPI1012752B1 (pt) 2009-04-20 2019-06-25 Oxford Nanopore Technologies Limited Método e aparelho para detectar uma interação de uma entidade molecular com uma proteína de membrana em uma camada de moléculas anfifílicas
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
EP2614156B1 (en) 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
WO2012098561A2 (en) 2011-01-18 2012-07-26 Swamy R K A multipupurpose instrument for triangle solutions, measurements and geometrical applications called triometer
WO2012098562A2 (en) 2011-01-19 2012-07-26 Panacea Biotec Limited Liquid oral compositions of lanthanum salts
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
RU2669844C2 (ru) 2013-03-14 2018-10-16 Аркема Инк. Способы сшивания полимерных композиций в присутствии атмосферного кислорода
CN118256603A (zh) 2013-10-18 2024-06-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US9925679B2 (en) 2014-05-19 2018-03-27 I+D+M Creative, Llc Devices and methods for assisting with slicing items

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