BR112015001052B8 - Métodos para distinguir um polinucleotídeo alvo,sensor para distinguir um polinucleotídeo alvo, e, uso de um construto - Google Patents
Métodos para distinguir um polinucleotídeo alvo,sensor para distinguir um polinucleotídeo alvo, e, uso de um construtoInfo
- Publication number
- BR112015001052B8 BR112015001052B8 BR112015001052A BR112015001052A BR112015001052B8 BR 112015001052 B8 BR112015001052 B8 BR 112015001052B8 BR 112015001052 A BR112015001052 A BR 112015001052A BR 112015001052 A BR112015001052 A BR 112015001052A BR 112015001052 B8 BR112015001052 B8 BR 112015001052B8
- Authority
- BR
- Brazil
- Prior art keywords
- polynucleotide
- distinguish
- target polynucleotide
- construct
- methods
- Prior art date
Links
- 102000040430 polynucleotide Human genes 0.000 title abstract 11
- 108091033319 polynucleotide Proteins 0.000 title abstract 11
- 239000002157 polynucleotide Substances 0.000 title abstract 11
- 238000000034 method Methods 0.000 title abstract 3
- 108060004795 Methyltransferase Proteins 0.000 abstract 2
- 230000004520 agglutination Effects 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
- C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261673446P | 2012-07-19 | 2012-07-19 | |
| US61/673,446 | 2012-07-19 | ||
| US201361774694P | 2013-03-08 | 2013-03-08 | |
| US61/774,694 | 2013-03-08 | ||
| PCT/GB2013/051928 WO2014013262A1 (en) | 2012-07-19 | 2013-07-18 | Enzyme construct |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112015001052A2 BR112015001052A2 (https=) | 2017-08-15 |
| BR112015001052B1 BR112015001052B1 (pt) | 2022-04-05 |
| BR112015001052B8 true BR112015001052B8 (pt) | 2022-12-27 |
Family
ID=48874433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015001052A BR112015001052B8 (pt) | 2012-07-19 | 2013-07-18 | Métodos para distinguir um polinucleotídeo alvo,sensor para distinguir um polinucleotídeo alvo, e, uso de um construto |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9797009B2 (https=) |
| EP (1) | EP2875152B1 (https=) |
| JP (2) | JP6429773B2 (https=) |
| AU (1) | AU2013291765C1 (https=) |
| BR (1) | BR112015001052B8 (https=) |
| CA (1) | CA2879355C (https=) |
| WO (1) | WO2014013262A1 (https=) |
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| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| EP4737389A2 (en) | 2011-05-27 | 2026-05-06 | Oxford Nanopore Technologies PLC | Coupling method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| KR20130049671A (ko) | 2011-11-04 | 2013-05-14 | 한미사이언스 주식회사 | 생리활성 폴리펩타이드 결합체 제조 방법 |
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| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
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| US9551023B2 (en) | 2012-09-14 | 2017-01-24 | Oxford Nanopore Technologies Ltd. | Sample preparation method |
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| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
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| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
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| GB201406151D0 (en) * | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN111534504B (zh) | 2014-01-22 | 2024-06-21 | 牛津纳米孔科技公开有限公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
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| MX387560B (es) * | 2014-03-31 | 2025-03-18 | Hanmi Pharmaceutical Co Ltd | Procedimiento para mejorar la solubilidad de proteínas y péptidos usando un enlace del fragmento fc de inmunoglobulina. |
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| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
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| EP4737389A2 (en) | 2011-05-27 | 2026-05-06 | Oxford Nanopore Technologies PLC | Coupling method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| US9617591B2 (en) * | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| US9644089B2 (en) | 2013-03-14 | 2017-05-09 | Arkema Inc. | Methods for crosslinking polymer compositions in the presence of atmospheric oxygen |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| US9925679B2 (en) | 2014-05-19 | 2018-03-27 | I+D+M Creative, Llc | Devices and methods for assisting with slicing items |
-
2013
- 2013-07-18 JP JP2015522172A patent/JP6429773B2/ja active Active
- 2013-07-18 WO PCT/GB2013/051928 patent/WO2014013262A1/en not_active Ceased
- 2013-07-18 EP EP13740351.5A patent/EP2875152B1/en active Active
- 2013-07-18 BR BR112015001052A patent/BR112015001052B8/pt active IP Right Grant
- 2013-07-18 US US14/415,533 patent/US9797009B2/en active Active
- 2013-07-18 AU AU2013291765A patent/AU2013291765C1/en active Active
- 2013-07-18 CA CA2879355A patent/CA2879355C/en active Active
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2017
- 2017-09-14 US US15/704,395 patent/US20180230526A1/en not_active Abandoned
-
2018
- 2018-08-03 JP JP2018146743A patent/JP2018186830A/ja active Pending
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|---|---|
| AU2013291765B2 (en) | 2019-05-16 |
| BR112015001052B1 (pt) | 2022-04-05 |
| BR112015001052A2 (https=) | 2017-08-15 |
| JP2018186830A (ja) | 2018-11-29 |
| CA2879355A1 (en) | 2014-01-23 |
| CA2879355C (en) | 2021-09-21 |
| AU2013291765A1 (en) | 2015-02-05 |
| EP2875152A1 (en) | 2015-05-27 |
| JP6429773B2 (ja) | 2018-11-28 |
| US20180230526A1 (en) | 2018-08-16 |
| US20150218629A1 (en) | 2015-08-06 |
| JP2015529451A (ja) | 2015-10-08 |
| US9797009B2 (en) | 2017-10-24 |
| WO2014013262A1 (en) | 2014-01-23 |
| AU2013291765C1 (en) | 2019-08-08 |
| EP2875152B1 (en) | 2019-10-09 |
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