CA2879355C - Helicase construct and its use in characterising polynucleotides - Google Patents
Helicase construct and its use in characterising polynucleotides Download PDFInfo
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- CA2879355C CA2879355C CA2879355A CA2879355A CA2879355C CA 2879355 C CA2879355 C CA 2879355C CA 2879355 A CA2879355 A CA 2879355A CA 2879355 A CA2879355 A CA 2879355A CA 2879355 C CA2879355 C CA 2879355C
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
- C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Molecular Biology (AREA)
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- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
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| US201261673446P | 2012-07-19 | 2012-07-19 | |
| US61/673,446 | 2012-07-19 | ||
| US201361774694P | 2013-03-08 | 2013-03-08 | |
| US61/774,694 | 2013-03-08 | ||
| PCT/GB2013/051928 WO2014013262A1 (en) | 2012-07-19 | 2013-07-18 | Enzyme construct |
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| CA2879355A1 CA2879355A1 (en) | 2014-01-23 |
| CA2879355C true CA2879355C (en) | 2021-09-21 |
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| CA2879355A Active CA2879355C (en) | 2012-07-19 | 2013-07-18 | Helicase construct and its use in characterising polynucleotides |
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|---|---|
| US (2) | US9797009B2 (https=) |
| EP (1) | EP2875152B1 (https=) |
| JP (2) | JP6429773B2 (https=) |
| AU (1) | AU2013291765C1 (https=) |
| BR (1) | BR112015001052B8 (https=) |
| CA (1) | CA2879355C (https=) |
| WO (1) | WO2014013262A1 (https=) |
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| WO2008102120A1 (en) | 2007-02-20 | 2008-08-28 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor system |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| JP5843614B2 (ja) | 2009-01-30 | 2016-01-13 | オックスフォード ナノポア テクノロジーズ リミテッド | 膜貫通配列決定における核酸構築物のためのアダプター |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| SG10201604316WA (en) | 2011-05-27 | 2016-07-28 | Oxford Nanopore Tech Ltd | Coupling method |
| AU2012288629B2 (en) | 2011-07-25 | 2017-02-02 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| EP3663412B1 (en) | 2011-09-23 | 2022-03-09 | Oxford Nanopore Technologies PLC | Analysis of a polymer comprising polymer units by means of translocation through a nanopore |
| JP6226869B2 (ja) | 2011-10-21 | 2017-11-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素法 |
| KR20130049671A (ko) | 2011-11-04 | 2013-05-14 | 한미사이언스 주식회사 | 생리활성 폴리펩타이드 결합체 제조 방법 |
| BR112014016112B8 (pt) | 2011-12-29 | 2022-12-27 | Oxford Nanopore Tech Ltd | Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| WO2013121201A1 (en) | 2012-02-15 | 2013-08-22 | Oxford Nanopore Technologies Limited | Aptamer method |
| BR112014020211A2 (pt) | 2012-02-16 | 2017-07-04 | Oxford Nanopore Tech Ltd | métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico |
| AR090281A1 (es) | 2012-03-08 | 2014-10-29 | Hanmi Science Co Ltd | Proceso mejorado para la preparacion de un complejo polipeptidico fisiologicamente activo |
| JP6271505B2 (ja) | 2012-04-10 | 2018-01-31 | オックスフォード ナノポール テクノロジーズ リミテッド | 変異体ライセニンポア |
| EP2875128B8 (en) | 2012-07-19 | 2020-06-24 | Oxford Nanopore Technologies Limited | Modified helicases |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| US9797009B2 (en) | 2012-07-19 | 2017-10-24 | Oxford Nanopore Technologies Limited | Enzyme construct |
| EP2895618B1 (en) | 2012-09-14 | 2017-07-26 | Oxford Nanopore Technologies Limited | Sample preparation method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| EP2917366B1 (en) | 2012-11-06 | 2017-08-02 | Oxford Nanopore Technologies Limited | Quadruplex method |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| KR102168813B1 (ko) | 2013-03-08 | 2020-10-22 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 정지 방법 |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN118256603A (zh) | 2013-10-18 | 2024-06-28 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| JP6749243B2 (ja) | 2014-01-22 | 2020-09-02 | オックスフォード ナノポール テクノロジーズ リミテッド | １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| AU2015242657B2 (en) * | 2014-03-31 | 2020-05-21 | Hanmi Pharm. Co., Ltd. | Method for improving solubility of protein and peptide by using immunoglobulin Fc fragment linkage |
| EP3126515B1 (en) | 2014-04-04 | 2018-08-29 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| US10400014B2 (en) | 2014-09-01 | 2019-09-03 | Oxford Nanopore Technologies Ltd. | Mutant CsgG pores |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| EP3971300B1 (en) | 2014-10-16 | 2024-06-05 | Oxford Nanopore Technologies plc | Sorting of polymers |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| GB201418512D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Electrical device with detachable components |
| KR102457147B1 (ko) | 2014-10-17 | 2022-10-19 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노 세공 rna 특징 규명을 위한 방법 |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| US10280411B2 (en) | 2015-10-27 | 2019-05-07 | Pacific Biosciences of California, In.c | Methods, systems, and reagents for direct RNA sequencing |
| KR102837999B1 (ko) | 2015-12-08 | 2025-07-24 | 카트호리이케 유니버시타이트 로이펜 | 변형된 나노세공, 그를 포함하는 조성물, 및 그의 용도 |
| AU2017225374B2 (en) | 2016-03-02 | 2020-03-05 | Oxford Nanopore Technologies Limited | Mutant pore |
| WO2017174990A1 (en) | 2016-04-06 | 2017-10-12 | Oxford Nanopore Technologies Limited | Mutant pore |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| EP4063521B1 (en) | 2016-05-25 | 2024-02-21 | Oxford Nanopore Technologies PLC | Method of nanopore sequencing |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| WO2019006214A1 (en) | 2017-06-29 | 2019-01-03 | President And Fellows Of Harvard College | DETERMINISTIC PROGRESSION OF POLYMERS THROUGH A NANOPORE |
| WO2019002893A1 (en) | 2017-06-30 | 2019-01-03 | Vib Vzw | NEW PROTEIN PORES |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| GB2569977A (en) | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB2574048B (en) | 2018-05-24 | 2021-06-16 | Oxford Nanopore Tech Ltd | Nanopore sensor component with electrostatic discharge protection |
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