JP2015527074A - 細胞表面におけるタンパク質の発現及びシグナル伝達を増大するための組成物及び方法 - Google Patents

細胞表面におけるタンパク質の発現及びシグナル伝達を増大するための組成物及び方法 Download PDF

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JP2015527074A
JP2015527074A JP2015530507A JP2015530507A JP2015527074A JP 2015527074 A JP2015527074 A JP 2015527074A JP 2015530507 A JP2015530507 A JP 2015530507A JP 2015530507 A JP2015530507 A JP 2015530507A JP 2015527074 A JP2015527074 A JP 2015527074A
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protein
tag
rho
lucy
vector
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JP2015527074A5 (enExample
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ジェニファー プラズニック，
ジェニファー プラズニック，
ニランジャナ ナタラジャン，
ニランジャナ ナタラジャン，
ブライス シェパード，
ブライス シェパード，
Original Assignee
ザ ジョンズ ホプキンズ ユニヴァーシティー
ザ ジョンズ ホプキンズ ユニヴァーシティー
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Immunology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2015530507A 2012-09-10 2013-09-26 細胞表面におけるタンパク質の発現及びシグナル伝達を増大するための組成物及び方法 Pending JP2015527074A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261698930P 2012-09-10 2012-09-10
PCT/IB2013/002242 WO2014037800A2 (en) 2012-09-10 2013-09-26 Compositions and methods for increasing the expression and signalling of proteins on cell surfaces

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JP2015527074A true JP2015527074A (ja) 2015-09-17
JP2015527074A5 JP2015527074A5 (enExample) 2016-11-04

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JP2015530507A Pending JP2015527074A (ja) 2012-09-10 2013-09-26 細胞表面におけるタンパク質の発現及びシグナル伝達を増大するための組成物及び方法

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US (1) US9783585B2 (enExample)
EP (1) EP2893020B1 (enExample)
JP (1) JP2015527074A (enExample)
AU (1) AU2013311316A1 (enExample)
WO (1) WO2014037800A2 (enExample)

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
US10435456B2 (en) * 2014-01-29 2019-10-08 The Johns Hopkins University Compositions and methods for increasing the expression and signalling of proteins on cell surfaces
GB202108867D0 (en) 2021-06-21 2021-08-04 Givaudan Sa Screening system
WO2024126820A1 (en) 2022-12-15 2024-06-20 Givaudan Sa Modified olfactory receptors
EP4664463A1 (en) 2024-06-14 2025-12-17 Givaudan SA Computer-implemented method for olfactory property prediction

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005506039A (ja) * 2000-12-29 2005-03-03 ネオジェネシス ファーマシューティカルズ インコーポレイテッド アフィニティ選択に基づいた疎水性タンパク質のスクリーニング
JP2008503220A (ja) * 2004-06-18 2008-02-07 デューク ユニバーシティー 匂い物質受容体のモジュレーター
WO2011069156A2 (en) * 2009-12-04 2011-06-09 Duke University Compositions and methods for enhancing odorant receptor activity
JP2012050411A (ja) * 2010-09-03 2012-03-15 Kao Corp 悪臭抑制剤の探索方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5993778A (en) * 1997-05-07 1999-11-30 Firestein; Stuart J. Functional expression of, and assay for, functional cellular receptors in vivo
US20040142325A1 (en) * 2001-09-14 2004-07-22 Liat Mintz Methods and systems for annotating biomolecular sequences
WO2006084327A1 (en) * 2005-02-09 2006-08-17 Apollo Life Sciences Limited A molecule and chimeric molecules thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005506039A (ja) * 2000-12-29 2005-03-03 ネオジェネシス ファーマシューティカルズ インコーポレイテッド アフィニティ選択に基づいた疎水性タンパク質のスクリーニング
JP2008503220A (ja) * 2004-06-18 2008-02-07 デューク ユニバーシティー 匂い物質受容体のモジュレーター
WO2011069156A2 (en) * 2009-12-04 2011-06-09 Duke University Compositions and methods for enhancing odorant receptor activity
JP2012050411A (ja) * 2010-09-03 2012-03-15 Kao Corp 悪臭抑制剤の探索方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALEX, V., ET AL.: "Influence of tags on ORs functional properties in HEK293 cells", CHEMICAL SENSES, vol. 36（９）, JPN7017002433, 2011, pages 70 - 233, ISSN: 0003607943 *
CHAN, DV., ET AL.: "Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein,", BMC BIOCHEMISTRY, vol. 12:27, JPN6017028013, 2011, ISSN: 0003780799 *
KRAUTWURST, D., ET AL.: "Identification of Ligands for Olfactory Receptors by Functional Expression of a Receptor Library", CELL, vol. 95, JPN6017028018, 1998, pages 917 - 926, XP002268732, ISSN: 0003607942, DOI: 10.1016/S0092-8674(00)81716-X *
ZHUANG H., ET AL.: "Synergism of Accessory Factors in Functional Expression of Mammalian Odorant Receptors", JBC, vol. 282（20）, JPN6017028016, 2007, pages 15284 - 15293, ISSN: 0003780800 *

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Publication number Publication date
EP2893020B1 (en) 2018-02-28
WO2014037800A3 (en) 2014-05-01
WO2014037800A2 (en) 2014-03-13
US9783585B2 (en) 2017-10-10
EP2893020A2 (en) 2015-07-15
US20150239945A1 (en) 2015-08-27
AU2013311316A1 (en) 2015-03-26
EP2893020A4 (en) 2016-09-21

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