JP2015527074A - 細胞表面におけるタンパク質の発現及びシグナル伝達を増大するための組成物及び方法 - Google Patents
細胞表面におけるタンパク質の発現及びシグナル伝達を増大するための組成物及び方法 Download PDFInfo
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Abstract
Description
材料及び方法
結果
考察
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3. Buck L, Axel R (1991) A novel multigenefamily may encode odorant receptors: a molecular basis for odor recognition.Cell 65: 175-187.
4. Katada S, Nakagawa T, Kataoka H, TouharaK (2003) Odorant response assays for a heterologously expressed olfactoryreceptor. Biochem Biophys Res Commun 305: 964-969.
5. Zhuang H, Matsunami H (2008) Evaluatingcell-surface expression and measuring activation of mammalian odorant receptorsin heterologous cells. Nat Protoc 3: 1402-1413.
6. Touhara K (2007) Deorphanizingvertebrate olfactory receptors: recent advances in odorant-response assays.Neurochem Int 51: 132-139.
7. Wetzel CH, Oles M, Wellerdieck C,Kuczkowiak M, Gisselmann G et al. (1999) Specificity and sensitivity of a humanolfactory receptor functionally expressed in human embryonic kidney 293 cellsand Xenopus Laevis oocytes. J Neurosci 19: 7426-7433.
8. Lu M, Echeverri F, Moyer BD (2003)Endoplasmic reticulum retention, degradation, and aggregation of olfactoryG-protein coupled receptors. Traffic 4: 416-433.
9. McClintock TS, Sammeta N (2003)Trafficking prerogatives of olfactory receptors. Neuroreport 14: 1547-1552.
10. Mombaerts P (2004) Genes and ligands forodorant, vomeronasal and taste receptors. Nat Rev Neurosci 5: 263-278.
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14. Hall RA (2009) Olfactory receptorinteractions with other receptors. Ann. N Y Acad Sci 1170: 147-149.
15. Krautwurst D, Yau KW, Reed RR (1998)Identification of ligands for olfactory receptors by functional expression of areceptor library. Cell 95: 917-926.
16. Saito H, Kubota M, Roberts RW, Chi Q,Matsunami H (2004) RTP family members induce functional expression of mammalianodorant receptors. Cell 119: 679-691.
17. Matsunami H, Mainland JD, Dey S (2009)Trafficking of mammalian chemosensory receptors by receptor-transportingproteins. Ann N Y Acad Sci 1170: 153-156.
18. Zhuang H, Matsunami H (2007) Synergismof accessory factors in functional expression of mammalian odorant receptors. JBiol Chem 282: 15284-15293.
19. Wu L, Pan Y, Chen GQ, Matsunami H,Zhuang H (2012) Receptor- transporting protein 1 short (RTP1S) mediatestranslocation and activation of odorant receptors by acting through multiplesteps. J Biol Chem 287: 22287-22294.
20. Von Dannecker LE, Mercadante AF, MalnicB (2006) Ric-8B promotes functional expression of odorant receptors. Proc NatlAcad Sci USA 103: 9310-9314.
21. Belluscio L, Gold GH, Nemes A, Axel R(1998) Mice deficient in G(olf) are anosmic. Neuron 20: 69-81.
22. Kajiya K, Inaki K, Tanaka M, Haga T,Kataoka H et al. (2001) Molecular bases of odor discrimination: Reconstitutionof olfactory receptors that recognize overlapping sets of odorants. J Neurosci21: 6018-6025.
23. Nara K, Saraiva LR, Ye X, Buck LB(2011) A large-scale analysis of odor coding in the olfactory epithelium. JNeurosci 31: 9179-9191.
24. Saito H, Chi Q, Zhuang H, Matsunami H,Mainland JD (2009) Odor coding by a Mammalian receptor repertoire. Sci Signal2: ra9.
25. Zimmermann R, Eyrisch S, Ahmad M, HelmsV (2011) Protein translocation across the ER membrane. Biochim Biophys Acta1808: 912-924.
26. Schulein R, Westendorf C, Krause G,Rosenthal W (2012) Functional significance of cleavable signal peptides of Gprotein-coupled receptors. Eur J Cell Biol 91: 294-299.
27. Zampatis DE, Rutz C, Furkert J, SchmidtA, Wustenhagen D et al. (2012) The protease-activated receptor 1 possesses afunctional and cleavable signal peptide which is necessary for receptorexpression. FEBS Lett 586: 2351-2359.
28. Chan DV, Somani AK, Young AB, MassariJV, Ohtola J et al. (2011) Signal peptide cleavage is essential for surfaceexpression of a regulatory T cell surface protein, leucine rich repeatcontaining 32 (LRRC32). BMC Biochem 12: 27: 1471-2091
29. Dunham JH, Hall RA (2009) Enhancementof the surface expression of G protein-coupled receptors. Trends Biotechnol 27:541-545.
30. Guan XM, Kobilka TS, Kobilka BK (1992)Enhancement of membrane insertion and function in a type IIIb membrane proteinfollowing introduction of a cleavable signal peptide. J Biol Chem 267:21995-21998.
31. Pluznick JL, Zou DJ, Zhang X, Yan Q,Rodriguez-Gil DJ et al. (2009) Functional expression of the olfactory signalingsystem in the kidney. Proc Natl Acad Sci USA 106: 2059-2064.
32. Bryksin AV, Matsumura I (2010) Overlapextension PCR cloning: a simple and reliable way to create recombinantplasmids. BioTechniques 48: 463-465.
33. Hansson MD, Rzeznicka K, Rosenback M,Hansson M, Sirijovski N (2008) PCR-mediated deletion of plasmid DNA. AnalBiochem 375: 373-375.
34. Chapin HC, Rajendran V, Capasso A,Caplan MJ (2009) Detecting the surface localization and cytoplasmic cleavage ofmembrane-bound proteins. Methods Cell Biol 94: 223-239.
35. Bush CF, Hall RA (2008) Olfactoryreceptor trafficking to the plasma membrane. Cell Mol Life Sci 65: 2289-2295.
36. Alken M, Schmidt A, Rutz C, Furkert J,Kleinau G et al. (2009) The sequence after the signal peptide of the Gprotein-coupled endothelin B receptor is required for efficient translocongating at the endoplasmic reticulum membrane. Mol Pharmacol 75: 801-811.
37. Jacquier V, Prummer M, Segura JM, PickH, Vogel H (2006) Visualizing odorant receptor trafficking in living cells downto the single-molecule level. Proc Natl Acad Sci USA 103: 14325-14330.
38. Griffin CA, Kafadar KA, Pavlath GK(2009) MOR23 promotes muscle regeneration and regulates cell adhesion andmigration. Dev Cell 17: 649-661.
39. Pavlath GK (2010) A new function forodorant receptors: MOR23 is necessary for normal tissue repair in skeletalmuscle. Cell Adh Migr 4: 502-506.
40. Spehr M, Gisselmann G, Poplawski A,Riffell JA, Wetzel CH et al. (2003) Identification of a testicular odorantreceptor mediating human sperm chemotaxis. Science 299: 2054-2058.
41. Spehr M, Schwane K, Riffell JA, ZimmerRK, Hatt H (2006) Odorant receptors and olfactory-like signaling mechanisms inmammalian sperm. Mol Cell Endocrinol 250: 128-136.
42. Zhang X, Rogers M, Tian H, Zhang X, ZouDJ et al. (2004) High- throughput microarray detection of olfactory receptorgene expression in the mouse. Proc Natl Acad Sci USA 101: 14168-14173.
43. Zhang X, De la Cruz O, Pinto JM,Nicolae D, Firestein S et al. (2007) Characterizing the expression of the humanolfactory receptor gene family using a novel DNA microarray. Genome Biol 8:R86.
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Claims (30)
- 配列番号1に示されるヌクレオチド配列。
- 配列番号2のアミノ酸配列をコードするヌクレオチド配列。
- 配列番号2に示されるアミノ酸配列。
- 配列番号3を含む、タンパク質を細胞表面に輸送するのに有用なN末端アミノ酸配列。
- Flagタグをさらに含む、請求項4に記載のN末端アミノ酸配列。
- Rhoタグをさらに含む、請求項4に記載のN末端アミノ酸配列。
- Rhoタグをさらに含む、請求項5に記載のN末端アミノ酸配列。
- FlagタグとRhoタグとの間にリンカーをさらに含む、請求項7に記載のN末端アミノ酸配列。
- 配列番号3と少なくとも90%の同一性を有するアミノ酸配列を含む、タンパク質を細胞表面に輸送するのに有用なN末端アミノ酸配列。
- Flagタグをさらに含む、請求項9に記載のN末端アミノ酸配列。
- Rhoタグをさらに含む、請求項9に記載のN末端アミノ酸配列。
- Rhoタグをさらに含む、請求項10に記載のN末端アミノ酸配列。
- FlagタグとRhoタグとの間にリンカーをさらに含む、請求項12に記載のN末端アミノ酸配列。
- タンパク質の表面発現を増大するためのシステムであって、
a.(i)請求項1若しくは2に記載のヌクレオチド配列、又は(ii)請求項3〜13のいずれか一項に記載の配列をコードするヌクレオチド配列を含む、ベクター;並びに
b.前記タンパク質の細胞表面への発現、シグナル伝達及び/又は輸送を補助するシャペロンタンパク質をコードするベクター
を含む、システム。 - 前記タンパク質が、嗅覚受容体である、請求項14に記載のシステム。
- 前記シャペロンタンパク質が、受容体輸送タンパク質である、請求項14に記載のシステム。
- 前記受容体輸送タンパク質が、RTPL1、RTP1S及びRTP2からなる群から選択される、請求項16に記載のシステム。
- 前記シャペロンタンパク質が、受容体発現増強タンパク質(REEP)である、請求項14に記載のシステム。
- 前記シャペロンタンパク質が、β−アドレナリン作動性受容体である、請求項14に記載のシステム。
- 前記シャペロンタンパク質が、熱ショックタンパク質70ホモログである、請求項14に記載のシステム。
- 前記シャペロンタンパク質が、コリンエステラーゼ8ホモログBのインヒビターに対して抵抗性である(Ric8b)、請求項14に記載のシステム。
- 前記シャペロンタンパク質が、嗅覚Gタンパク質(Gαolf)である、請求項14に記載のシステム。
- 細胞株をさらに含む、請求項14に記載のシステム。
- a.配列番号3をコードするヌクレオチド配列を含むベクター;
b.RTP1Sをコードするベクター;
c.Ric8bをコードするベクター;及び
d.Gαolfをコードするベクター
を含む、タンパク質の表面発現を増大するためのシステム。 - ステップ(a)の前記ベクターが、Flagタグをコードするヌクレオチド配列をさらに含む、請求項24に記載のシステム。
- ステップ(a)の前記ベクターが、Rhoタグをコードするヌクレオチド配列をさらに含む、請求項24に記載のシステム。
- ステップ(a)の前記ベクターが、Rhoタグをコードするヌクレオチド配列をさらに含む、請求項25に記載のシステム。
- ステップ(a)の前記ベクターが、FlagタグとRhoタグとの間にリンカーをさらに含む、請求項27に記載のシステム。
- a.配列番号2をコードするヌクレオチド配列を含むベクター;
b.RTP1Sをコードするベクター;
c.Ric8bをコードするベクター;及び
d.Gαolfをコードするベクター
を含む、タンパク質の表面発現を増大するためのシステム。 - a.配列番号2をコードするヌクレオチド配列を含むベクター;
b.RTP1Sをコードするベクター;
c.Ric8bをコードするベクター;及び
d.Gαolfをコードするベクター
を含む、嗅覚受容体の表面発現を増大するためのシステム。
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US201261698930P | 2012-09-10 | 2012-09-10 | |
PCT/IB2013/002242 WO2014037800A2 (en) | 2012-09-10 | 2013-09-26 | Compositions and methods for increasing the expression and signalling of proteins on cell surfaces |
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GB202108867D0 (en) | 2021-06-21 | 2021-08-04 | Givaudan Sa | Screening system |
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JP2005506039A (ja) * | 2000-12-29 | 2005-03-03 | ネオジェネシス ファーマシューティカルズ インコーポレイテッド | アフィニティ選択に基づいた疎水性タンパク質のスクリーニング |
JP2008503220A (ja) * | 2004-06-18 | 2008-02-07 | デューク ユニバーシティー | 匂い物質受容体のモジュレーター |
WO2011069156A2 (en) * | 2009-12-04 | 2011-06-09 | Duke University | Compositions and methods for enhancing odorant receptor activity |
JP2012050411A (ja) * | 2010-09-03 | 2012-03-15 | Kao Corp | 悪臭抑制剤の探索方法 |
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US5993778A (en) * | 1997-05-07 | 1999-11-30 | Firestein; Stuart J. | Functional expression of, and assay for, functional cellular receptors in vivo |
US20040142325A1 (en) * | 2001-09-14 | 2004-07-22 | Liat Mintz | Methods and systems for annotating biomolecular sequences |
WO2006084327A1 (en) * | 2005-02-09 | 2006-08-17 | Apollo Life Sciences Limited | A molecule and chimeric molecules thereof |
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JP2005506039A (ja) * | 2000-12-29 | 2005-03-03 | ネオジェネシス ファーマシューティカルズ インコーポレイテッド | アフィニティ選択に基づいた疎水性タンパク質のスクリーニング |
JP2008503220A (ja) * | 2004-06-18 | 2008-02-07 | デューク ユニバーシティー | 匂い物質受容体のモジュレーター |
WO2011069156A2 (en) * | 2009-12-04 | 2011-06-09 | Duke University | Compositions and methods for enhancing odorant receptor activity |
JP2012050411A (ja) * | 2010-09-03 | 2012-03-15 | Kao Corp | 悪臭抑制剤の探索方法 |
Non-Patent Citations (4)
Title |
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ALEX, V., ET AL.: "Influence of tags on ORs functional properties in HEK293 cells", CHEMICAL SENSES, vol. 36(9), JPN7017002433, 2011, pages 70 - 233, ISSN: 0003607943 * |
CHAN, DV., ET AL.: "Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein,", BMC BIOCHEMISTRY, vol. 12:27, JPN6017028013, 2011, ISSN: 0003780799 * |
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ZHUANG H., ET AL.: "Synergism of Accessory Factors in Functional Expression of Mammalian Odorant Receptors", JBC, vol. 282(20), JPN6017028016, 2007, pages 15284 - 15293, ISSN: 0003780800 * |
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AU2013311316A1 (en) | 2015-03-26 |
EP2893020B1 (en) | 2018-02-28 |
US9783585B2 (en) | 2017-10-10 |
US20150239945A1 (en) | 2015-08-27 |
WO2014037800A2 (en) | 2014-03-13 |
EP2893020A2 (en) | 2015-07-15 |
EP2893020A4 (en) | 2016-09-21 |
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