JP2015505979A - 高い空間的および／または時間的精度での撮像のためのシステムおよび方法 - Google Patents

高い空間的および／または時間的精度での撮像のためのシステムおよび方法 Download PDF

Info

Publication number
JP2015505979A
JP2015505979A JP2014543566A JP2014543566A JP2015505979A JP 2015505979 A JP2015505979 A JP 2015505979A JP 2014543566 A JP2014543566 A JP 2014543566A JP 2014543566 A JP2014543566 A JP 2014543566A JP 2015505979 A JP2015505979 A JP 2015505979A
Authority
JP
Japan
Prior art keywords
optical system
imaging
fluorescence
cell
voltage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP2014543566A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015505979A5 (enExample
Inventor
コーエン，アダム，イー．
マクローリン，ドゥーガル
ホッホバウム，ダニエル
クラリ，ジョエル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of JP2015505979A publication Critical patent/JP2015505979A/ja
Publication of JP2015505979A5 publication Critical patent/JP2015505979A5/ja
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G02B21/0084—Details of detection or image processing, including general computer control time-scale detection, e.g. strobed, ultra-fast, heterodyne detection
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G02B21/025—Objectives with variable magnification
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers

Landscapes

  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2014543566A 2011-11-23 2012-11-21 高い空間的および／または時間的精度での撮像のためのシステムおよび方法 Ceased JP2015505979A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161563537P 2011-11-23 2011-11-23
US61/563,537 2011-11-23
PCT/US2012/066303 WO2013078347A2 (en) 2011-11-23 2012-11-21 Systems and methods for imaging at high spatial and/or temporal precision

Publications (2)

Publication Number Publication Date
JP2015505979A true JP2015505979A (ja) 2015-02-26
JP2015505979A5 JP2015505979A5 (enExample) 2016-01-14

Family

ID=47430059

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014543566A Ceased JP2015505979A (ja) 2011-11-23 2012-11-21 高い空間的および／または時間的精度での撮像のためのシステムおよび方法

Country Status (5)

Country Link
US (1) US20150004637A1 (enExample)
EP (1) EP2798391A2 (enExample)
JP (1) JP2015505979A (enExample)
CA (1) CA2856423A1 (enExample)
WO (1) WO2013078347A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021167731A (ja) * 2020-04-09 2021-10-21 国立大学法人 東京大学 膜電位センサー
JP2024500089A (ja) * 2020-12-10 2024-01-04 サントル ナシオナル ドゥ ラ ルシェルシェ シアンティフィク 光子の再割り当てを行う共焦点顕微鏡

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8741591B2 (en) 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
US9207237B2 (en) 2010-08-23 2015-12-08 President And Fellows Of Harvard College Systems, methods, and workflows for optogenetics analysis
CA2838330C (en) 2010-08-23 2021-01-26 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
JP6284372B2 (ja) * 2014-01-21 2018-02-28 オリンパス株式会社 走査型レーザ顕微鏡および超解像画像生成方法
CA2946378A1 (en) * 2014-04-22 2015-10-29 Q-State Biosciences, Inc. Analysis of compounds for pain and sensory disorders
EP3158338B1 (en) 2014-06-18 2020-03-11 President and Fellows of Harvard College Optogenetic probes for measuring membrane potential
WO2016115333A1 (en) 2015-01-15 2016-07-21 President And Fellows Of Harvard College Optical selection of cells
US10048275B2 (en) 2015-03-13 2018-08-14 Q-State Biosciences, Inc. Cardiotoxicity screening methods
WO2016187543A1 (en) 2015-05-21 2016-11-24 Q-State Biosciences, Inc. Optogenetics microscope
DE102015111817B4 (de) * 2015-07-21 2019-11-07 Stiftung Caesar Center Of Advanced European Studies And Research Vorrichtung zur Beobachtung der dreidimensionalen Bewegung von Objekten, die in einer Flüssigkeit gehalten sind
US10430810B2 (en) * 2015-09-22 2019-10-01 Health Care Direct, Inc. Systems and methods for assessing the marketability of a product
US10520437B1 (en) * 2019-05-24 2019-12-31 Raytheon Company High sensitivity sensor utilizing ultra-fast laser pulse excitation and time delayed detector
WO2021178041A1 (en) * 2020-03-02 2021-09-10 Massachusetts Institute Of Technology Phase cancellation microscopy
GB2593194B (en) * 2020-03-18 2022-09-07 Refeyn Ltd Methods and apparatus for optimised interferometric scattering microscopy
US12478971B2 (en) 2020-12-31 2025-11-25 Quiver Holdings Inc. Optical plate controller and reader
CA3206828A1 (en) 2020-12-31 2022-07-07 Q-State Biosciences, Inc. Calibrating and normalizing an imager for optogenetics
JP7900889B2 (ja) 2020-12-31 2026-08-05 キュー－ステート バイオサイエンシーズ， インコーポレイテッド 空間撮像およびビームホモジナイザを伴う顕微鏡
WO2022227091A1 (zh) * 2021-04-30 2022-11-03 深圳华大智造科技股份有限公司 视场拼接系统及方法、生物样品识别裝置及方法
EP4363832A4 (en) 2021-06-30 2025-05-07 Q-State Biosciences, Inc. MULTIPLEX OPTICAL STIMULUS AND READOUT PROCESS
EP4367500A4 (en) * 2021-07-08 2024-10-30 Ramot at Tel-Aviv University Ltd. SUPER TEMPORAL RESOLUTION
US20230045152A1 (en) * 2021-08-06 2023-02-09 Ramona Optics Inc. System and method to simultaneously track multiple organisms at high resolution
US20250216378A1 (en) 2023-12-28 2025-07-03 Biobridge Global Methods for performing miniaturized dynamic assays using microfluidics and related systems
US20260065454A1 (en) * 2024-08-29 2026-03-05 Universities Space Research Association Method and Device for Portable, Modular and Compact Optical Microscopy for Studying Material Dynamics at the Nanoscale

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0918772A (ja) * 1995-06-27 1997-01-17 Olympus Optical Co Ltd 顕微鏡画像解析装置
WO2007139201A1 (ja) * 2006-05-31 2007-12-06 Olympus Corporation 生体試料撮像方法および生体試料撮像装置
JP2009065848A (ja) * 2007-09-10 2009-04-02 Olympus Corp 遺伝子発現解析方法および遺伝子発現解析システム
WO2010027446A2 (en) * 2008-09-04 2010-03-11 Galenea Corp., Et Al. Synaptic vesicle cycling assays and systems
JP2010538603A (ja) * 2007-06-05 2010-12-16 ゼネラル・エレクトリック・カンパニイ 細胞の動きの自動的特徴づけ
JP2012014066A (ja) * 2010-07-02 2012-01-19 Olympus Corp 細胞観察方法および走査型顕微鏡装置

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4184917A (en) 1974-04-01 1980-01-22 Sandoz Ltd. Process for producing a structurally modified interferon
US4321365A (en) 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4293652A (en) 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
US4351901A (en) 1980-03-24 1982-09-28 Cetus Corporation Method for single nucleotide alteration
JP2002031758A (ja) * 2000-07-17 2002-01-31 Olympus Optical Co Ltd 顕微鏡
CA2838330C (en) 2010-08-23 2021-01-26 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0918772A (ja) * 1995-06-27 1997-01-17 Olympus Optical Co Ltd 顕微鏡画像解析装置
WO2007139201A1 (ja) * 2006-05-31 2007-12-06 Olympus Corporation 生体試料撮像方法および生体試料撮像装置
JP2010538603A (ja) * 2007-06-05 2010-12-16 ゼネラル・エレクトリック・カンパニイ 細胞の動きの自動的特徴づけ
JP2009065848A (ja) * 2007-09-10 2009-04-02 Olympus Corp 遺伝子発現解析方法および遺伝子発現解析システム
WO2010027446A2 (en) * 2008-09-04 2010-03-11 Galenea Corp., Et Al. Synaptic vesicle cycling assays and systems
JP2012014066A (ja) * 2010-07-02 2012-01-19 Olympus Corp 細胞観察方法および走査型顕微鏡装置

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021167731A (ja) * 2020-04-09 2021-10-21 国立大学法人 東京大学 膜電位センサー
JP2024500089A (ja) * 2020-12-10 2024-01-04 サントル ナシオナル ドゥ ラ ルシェルシェ シアンティフィク 光子の再割り当てを行う共焦点顕微鏡
JP7812376B2 (ja) 2020-12-10 2026-02-09 サントル ナシオナル ドゥ ラ ルシェルシェ シアンティフィク 光子の再割り当てを行う共焦点顕微鏡
US12546980B2 (en) 2020-12-10 2026-02-10 Centre National De La Reserche Scientifique Confocal microscope with photon re-allocation

Also Published As

Publication number Publication date
WO2013078347A3 (en) 2013-08-01
CA2856423A1 (en) 2013-05-30
WO2013078347A2 (en) 2013-05-30
US20150004637A1 (en) 2015-01-01
EP2798391A2 (en) 2014-11-05

Similar Documents

Publication Publication Date Title
US20150004637A1 (en) Systems and methods for imaging at high spatial and/or temporal precision
Li et al. Engineering of NEMO as calcium indicators with large dynamics and high sensitivity
Svoboda et al. Principles of two-photon excitation microscopy and its applications to neuroscience
JP5953293B2 (ja) 発光タンパクによる長期モニタリング方法および解析方法
Ramadass et al. Spectrally and spatially resolved fluorescence lifetime imaging in living cells: TRPV4–microfilament interactions
US20150226755A1 (en) Green-to-red photo-convertible fluorescent calcium indicator
Gensch et al. Determination of intracellular chloride concentrations by fluorescence lifetime imaging
US11041806B2 (en) Systems and methods for detection
Sheng et al. Comprehensive review of indicators and techniques for optical mapping of intracellular calcium ions
Liu et al. The F-techniques: advances in receptor protein studies
Alspaugh et al. Developing analysis protocols for monitoring intracellular oxygenation using fluorescence lifetime imaging of myoglobin-mCherry
US20140170769A1 (en) Microscope apparatus for detecting or imaging protein using probe for intrinsic fluorescence resonance energy transfer and method for detecting or imaging protein using the same
Evans et al. Imaging neuronal signal transduction using multiphoton FRET-FLIM
JP2005207823A (ja) 蛍光分光分析の方法
Blunck A Historical Outline of Fluorescence Spectroscopy in Ion Channel Research
Škerle et al. Dimerization of rhomboid protease RHBDL2 in lipid membranes addressed by FRET with MC simulations
Bhupathi et al. Single Molecule Imaging Using State-of-the-Art Microscopy Techniques
Hedrick et al. Imaging signaling transduction in single dendritic spines
Wei et al. Visualizing the structural dynamics of G protein-activated A2A adenosine receptor at the single-molecule level
Liu et al. Druggable and allosteric pocket prints of G-protein coupled receptors
Van Ye et al. Simultaneous electrical and single-molecule fluorescence detection in windowed microelectrode cavity arrays
Hink Fluorescence fluctuation spectroscopy applied to living plant cells
Hernandez Cabrera Adhesion mediated pre-activation of T cells
Hamzeh Organization and function of signaling molecules in sperm
Liu Investigations into Proprotein convertase subtilisin/kexin type 9 regulations of cell membrane Low density lipoprotein receptor properties using image correlation spectroscopy (ICS)

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151116

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151116

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20161021

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20161202

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170302

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170427

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20171003

A045 Written measure of dismissal of application [lapsed due to lack of payment]

Free format text: JAPANESE INTERMEDIATE CODE: A045

Effective date: 20180223