JP2015232462A - 分析方法 - Google Patents
分析方法 Download PDFInfo
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- JP2015232462A JP2015232462A JP2014118850A JP2014118850A JP2015232462A JP 2015232462 A JP2015232462 A JP 2015232462A JP 2014118850 A JP2014118850 A JP 2014118850A JP 2014118850 A JP2014118850 A JP 2014118850A JP 2015232462 A JP2015232462 A JP 2015232462A
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- skin
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- stratum corneum
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- 238000004458 analytical method Methods 0.000 title abstract description 16
- 150000002500 ions Chemical class 0.000 claims abstract description 40
- 239000002390 adhesive tape Substances 0.000 claims abstract description 36
- 238000002360 preparation method Methods 0.000 claims abstract description 31
- 238000005011 time of flight secondary ion mass spectroscopy Methods 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 21
- 102000011782 Keratins Human genes 0.000 claims abstract description 12
- 108010076876 Keratins Proteins 0.000 claims abstract description 12
- 210000003491 skin Anatomy 0.000 claims description 64
- 210000000434 stratum corneum Anatomy 0.000 claims description 32
- 210000002510 keratinocyte Anatomy 0.000 claims description 25
- 238000013507 mapping Methods 0.000 claims description 15
- 230000000699 topical effect Effects 0.000 claims description 2
- 238000002042 time-of-flight secondary ion mass spectrometry Methods 0.000 abstract description 4
- 238000004949 mass spectrometry Methods 0.000 abstract 1
- JUIUXBHZFNHITF-IEOSBIPESA-N [(2r)-2,5,7,8-tetramethyl-2-[(4r,8r)-4,8,12-trimethyltridecyl]-3,4-dihydrochromen-6-yl] dihydrogen phosphate Chemical compound OP(=O)(O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C JUIUXBHZFNHITF-IEOSBIPESA-N 0.000 description 20
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 19
- 229910052708 sodium Inorganic materials 0.000 description 19
- 239000011734 sodium Substances 0.000 description 19
- -1 ascorbyl magnesium phosphate Chemical compound 0.000 description 15
- 229960002261 magnesium phosphate Drugs 0.000 description 15
- 229910000157 magnesium phosphate Inorganic materials 0.000 description 15
- 239000004137 magnesium phosphate Substances 0.000 description 15
- 235000010994 magnesium phosphates Nutrition 0.000 description 15
- 239000007864 aqueous solution Substances 0.000 description 10
- 210000000736 corneocyte Anatomy 0.000 description 10
- 238000005259 measurement Methods 0.000 description 8
- 230000035699 permeability Effects 0.000 description 7
- 229920000742 Cotton Polymers 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 5
- 239000010931 gold Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000004299 exfoliation Methods 0.000 description 3
- 229940078752 magnesium ascorbyl phosphate Drugs 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- HTJNEBVCZXHBNJ-XCTPRCOBSA-H trimagnesium;(2r)-2-[(1s)-1,2-dihydroxyethyl]-3,4-dihydroxy-2h-furan-5-one;diphosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.OC[C@H](O)[C@H]1OC(=O)C(O)=C1O HTJNEBVCZXHBNJ-XCTPRCOBSA-H 0.000 description 3
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000000245 forearm Anatomy 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920000298 Cellophane Polymers 0.000 description 1
- GYHNNYVSQQEPJS-UHFFFAOYSA-N Gallium Chemical compound [Ga] GYHNNYVSQQEPJS-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 229910052797 bismuth Inorganic materials 0.000 description 1
- JCXGWMGPZLAOME-UHFFFAOYSA-N bismuth atom Chemical compound [Bi] JCXGWMGPZLAOME-UHFFFAOYSA-N 0.000 description 1
- 229910052792 caesium Inorganic materials 0.000 description 1
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 229910052733 gallium Inorganic materials 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 239000002952 polymeric resin Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- PBSRSWFGYPZDAU-FFIPNUABSA-H trimagnesium;[(2r)-2-[(1s)-1,2-dihydroxyethyl]-3-oxido-5-oxo-2h-furan-4-yl] phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].OC[C@H](O)[C@H]1OC(=O)C(OP([O-])([O-])=O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(OP([O-])([O-])=O)=C1[O-] PBSRSWFGYPZDAU-FFIPNUABSA-H 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Abstract
Description
(試料の塗布及び角質細胞の剥離)
被験者の前腕内側部2.5cm×3.5cmに1%リン酸アスコルビルマグネシウムを含む水溶液2mLを含浸させたコットンを貼付し、5分間静置した。コットンを取り除いた後、新しいコットンで皮膚表面に残った試料を取り除き、5分間静置した。その後、塗布部位よりメンディングテープ(住友3M)を用いて、連続7回テープストリップをして、角質細胞を剥離し、得られたテープを標本とした。コントロールは、隣接部位にリン酸アスコルビルマグネシウムを含まない製剤を用いて同じ実験を行った。
7層分の角質細胞を剥離した7枚の粘着テープを標本とし、TOF−SIMSにより分析を行った。検出条件を以下に示す。
(1)装置:TRIFT−III(アルバックファイ社製)
(2)測定条件:
高分解能イメージングモード
1次イオン 197Au1+ :22kV(current:1.2nA、Pulse width 11ns)
検出:Positive
測定範囲:300×300μm2、256×256pixel、20分積算
(試料の塗布及び角質細胞の剥離)
被験者の前腕内側部2.5cm×3.5cmに0.5%トコフェリルリン酸ナトリウムを含む水溶液2mLを含浸させたコットンを貼付し、5分間静置した。コットンを取り除いた後、新しいコットンで皮膚表面に残った試料を取り除き、60分間静置した。その後、塗布部位よりメンディングテープ(住友3M)を用いて、連続7回テープストリップをして、角質細胞を剥離し、得られたテープを標本とした。コントロールは、隣接部位にトコフェリルリン酸ナトリウムを含まない製剤を用いて同じ実験を行った。
7層分の角質細胞を剥離した7枚の粘着テープを標本とし、TOF−SIMSにより分析を行った。検出条件を以下に示す。
(1)装置:TRIFT−III(アルバックファイ社製)
(2)測定条件:
高分解能イメージングモード
1次イオン 197Au1+ :22kV(current:1.2nA、Pulse width 11ns)
検出:Negative
測定範囲:300×300μm2、256×256pixel、20分積算
Claims (3)
- 皮膚に浸透した皮膚外用剤の成分を分析する方法であって、
皮膚外用剤を皮膚に塗布する工程と、
皮膚外用剤が塗布された該皮膚の領域から粘着テープを用いて複数層の角質を剥離する工程と、
TOF−SIMSを用いて、該角質が付着した粘着テープから生成したイオンを質量分析する工程とを有することを特徴とする分析方法。 - 角質が付着した粘着テープから生成したイオンが、皮膚に塗布した皮膚外用剤の成分、角質細胞及びテープから選ばれた1種類以上のイオンである、請求項1記載の方法。
- 角質が付着した粘着テープから生成したイオンの分布を平面上にマッピングする工程を含む請求項1又は2記載の方法。
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JP2014118850A JP6530165B2 (ja) | 2014-06-09 | 2014-06-09 | 分析方法 |
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JP2014118850A JP6530165B2 (ja) | 2014-06-09 | 2014-06-09 | 分析方法 |
Publications (2)
Publication Number | Publication Date |
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JP2015232462A true JP2015232462A (ja) | 2015-12-24 |
JP6530165B2 JP6530165B2 (ja) | 2019-06-12 |
Family
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JP2014118850A Active JP6530165B2 (ja) | 2014-06-09 | 2014-06-09 | 分析方法 |
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JP (1) | JP6530165B2 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20180017467A (ko) * | 2016-08-09 | 2018-02-21 | (주)아모레퍼시픽 | 화장료 조성물들의 동일성 판별 방법 |
KR20200028726A (ko) * | 2018-09-07 | 2020-03-17 | 한국과학기술연구원 | 이차이온 질량분석기 및 주성분 분석을 이용한 흔적증거물 감식방법 |
CN112229699A (zh) * | 2020-09-27 | 2021-01-15 | 胜科纳米(苏州)有限公司 | 一种用于动态二次离子质谱(d-sims)分析的样品制备方法 |
Citations (5)
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JP2010261939A (ja) * | 2009-04-10 | 2010-11-18 | Canon Inc | 質量画像の形成方法 |
US20110071123A1 (en) * | 2009-09-18 | 2011-03-24 | James Robert Schwartz | Noninvasive Method for Measuring Histamine From Skin as an Objective Measurement of Itch |
JP2013015516A (ja) * | 2011-06-07 | 2013-01-24 | Shiseido Co Ltd | 分析方法及び分析キット |
US20130035395A1 (en) * | 2010-04-07 | 2013-02-07 | Fujifilm Corporation | Water-based cosmetic and manufacturing method thereof |
JP2014013201A (ja) * | 2012-07-04 | 2014-01-23 | Shiseido Co Ltd | 成分分布可視化装置、成分分布可視化方法、及び成分分布可視化プログラム |
-
2014
- 2014-06-09 JP JP2014118850A patent/JP6530165B2/ja active Active
Patent Citations (6)
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JP2010261939A (ja) * | 2009-04-10 | 2010-11-18 | Canon Inc | 質量画像の形成方法 |
US20110071123A1 (en) * | 2009-09-18 | 2011-03-24 | James Robert Schwartz | Noninvasive Method for Measuring Histamine From Skin as an Objective Measurement of Itch |
JP2013504759A (ja) * | 2009-09-18 | 2013-02-07 | ザ プロクター アンド ギャンブル カンパニー | 痒みの客観的測定値として皮膚からヒスタミンを測定するための非侵襲的方法 |
US20130035395A1 (en) * | 2010-04-07 | 2013-02-07 | Fujifilm Corporation | Water-based cosmetic and manufacturing method thereof |
JP2013015516A (ja) * | 2011-06-07 | 2013-01-24 | Shiseido Co Ltd | 分析方法及び分析キット |
JP2014013201A (ja) * | 2012-07-04 | 2014-01-23 | Shiseido Co Ltd | 成分分布可視化装置、成分分布可視化方法、及び成分分布可視化プログラム |
Non-Patent Citations (2)
Title |
---|
"TOF-SIMS(飛行時間型質量分析)顕微鏡を用いた角質バリア機能可視化法の開発", 科学研究費補助金研究成果報告書, JPN6018016484, 2013, ISSN: 0003919317 * |
AMY M. JUDD: "Distribution and Visualisation of Chlorhexidine Within the Skin Using ToF-SIMS: A Potential Platform", PHARMACEUTICAL RESEARCH, vol. Vol.30/Iss.7, JPN6018044798, 1 May 2013 (2013-05-01), pages 1896 - 1905, ISSN: 0003919316 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20180017467A (ko) * | 2016-08-09 | 2018-02-21 | (주)아모레퍼시픽 | 화장료 조성물들의 동일성 판별 방법 |
KR102587336B1 (ko) | 2016-08-09 | 2023-10-12 | (주)아모레퍼시픽 | 화장료 조성물들의 동일성 판별 방법 |
KR20200028726A (ko) * | 2018-09-07 | 2020-03-17 | 한국과학기술연구원 | 이차이온 질량분석기 및 주성분 분석을 이용한 흔적증거물 감식방법 |
KR102101484B1 (ko) * | 2018-09-07 | 2020-04-16 | 한국과학기술연구원 | 이차이온 질량분석기 및 주성분 분석을 이용한 흔적증거물 감식방법 |
CN112229699A (zh) * | 2020-09-27 | 2021-01-15 | 胜科纳米(苏州)有限公司 | 一种用于动态二次离子质谱(d-sims)分析的样品制备方法 |
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