JP2014513557A - 標的核酸を検出する方法及び組成物 - Google Patents

標的核酸を検出する方法及び組成物 Download PDF

Info

Publication number
JP2014513557A
JP2014513557A JP2014511555A JP2014511555A JP2014513557A JP 2014513557 A JP2014513557 A JP 2014513557A JP 2014511555 A JP2014511555 A JP 2014511555A JP 2014511555 A JP2014511555 A JP 2014511555A JP 2014513557 A JP2014513557 A JP 2014513557A
Authority
JP
Japan
Prior art keywords
target
probe
nucleic acid
ligation
linking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2014511555A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014513557A5 (OSRAM
Inventor
テルブルーゲン,ロバート
リウ,イェンボウ
チルダーズ,ジョン,レイ
キム,チャン,ヒ
アール. アベディ,マジド
Original Assignee
ディクステリティー ダイアグノスティクス インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ディクステリティー ダイアグノスティクス インコーポレイテッド filed Critical ディクステリティー ダイアグノスティクス インコーポレイテッド
Publication of JP2014513557A publication Critical patent/JP2014513557A/ja
Publication of JP2014513557A5 publication Critical patent/JP2014513557A5/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014511555A 2011-05-17 2012-05-17 標的核酸を検出する方法及び組成物 Pending JP2014513557A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161486817P 2011-05-17 2011-05-17
US61/486,817 2011-05-17
PCT/US2012/038436 WO2012158967A1 (en) 2011-05-17 2012-05-17 Methods and compositions for detecting target nucleic acids

Publications (2)

Publication Number Publication Date
JP2014513557A true JP2014513557A (ja) 2014-06-05
JP2014513557A5 JP2014513557A5 (OSRAM) 2015-05-21

Family

ID=46168641

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014511555A Pending JP2014513557A (ja) 2011-05-17 2012-05-17 標的核酸を検出する方法及び組成物

Country Status (9)

Country Link
US (3) US10066257B2 (OSRAM)
EP (1) EP2710145B1 (OSRAM)
JP (1) JP2014513557A (OSRAM)
KR (1) KR102085242B1 (OSRAM)
CN (2) CN106434871B (OSRAM)
AU (1) AU2012255121B2 (OSRAM)
CA (1) CA2836577C (OSRAM)
DK (1) DK2710145T3 (OSRAM)
WO (1) WO2012158967A1 (OSRAM)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180039643A (ko) * 2015-07-17 2018-04-18 나노스트링 테크놀로지스, 인크. 절편화된 조직의 사용자-한정된 영역에서의 복수의 단백질의 동시적인 정량화
JP2022506752A (ja) * 2018-11-05 2022-01-17 サントル・アンリ・ベクレル がんを診断するための方法及び関連するキット
JP2022145605A (ja) * 2021-03-18 2022-10-04 ゲノミル ヘルス オサケユイチア 希釈または非精製試料における核酸の正確な並行定量のための方法
JP2023545218A (ja) * 2020-10-06 2023-10-26 マテリアルズ アンド マシーンズ コーポレーション オブ アメリカ 生物学的試料からのrna又はdnaの検出のための方法

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007133703A2 (en) * 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012255121B2 (en) * 2011-05-17 2017-03-09 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
US9777322B2 (en) 2011-07-08 2017-10-03 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9080167B2 (en) * 2012-11-16 2015-07-14 Covaris, Inc. System and method for processing paraffin embedded samples
JP6484177B2 (ja) * 2012-12-07 2019-03-13 インヴァイティ コーポレイションInvitae Corporation 多重核酸検出方法
KR101829668B1 (ko) * 2013-03-27 2018-02-14 김성천 생체분자와 단일가닥핵산의 결합정보를 생성하기 위한 기준물질 및 핵산칩, 이들의 제조방법 및 이들을 이용한 생체분자 분석방법 및 장치
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
FI2992115T4 (fi) 2013-04-30 2025-05-15 California Institute Of Technology Molekyylien limitetty leimaus sekventiaalisella hybridisaatioviivakoodauksella
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
CA2951561C (en) 2014-06-10 2023-04-18 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
US10870845B2 (en) 2014-07-01 2020-12-22 Global Life Sciences Solutions Operations UK Ltd Methods for capturing nucleic acids
US11091810B2 (en) * 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US10683534B2 (en) 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US9856521B2 (en) * 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
WO2016094148A2 (en) * 2014-12-11 2016-06-16 General Electric Company Methods for capturing nucleic acids
CN105803055A (zh) * 2014-12-31 2016-07-27 天昊生物医药科技(苏州)有限公司 一种基于多重循环延伸连接的靶基因区域富集新方法
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
EP3325669B1 (en) * 2015-07-24 2023-07-05 The Johns Hopkins University Compositions and methods of rna analysis
WO2017027620A1 (en) * 2015-08-10 2017-02-16 Life Technologies Corporation Devices and methods for laser capture microdissection
US12421540B2 (en) 2016-08-01 2025-09-23 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
US10451599B2 (en) 2016-09-02 2019-10-22 Zepto Life Technology, LLC Methods of isolating and detecting metal ions from proteins
WO2019046768A1 (en) * 2017-08-31 2019-03-07 William Marsh Rice University SYMBOLIC SEQUENCING OF DNA AND RNA BY SEQUENCE CODING
EP4589017A3 (en) 2017-10-06 2025-10-08 10x Genomics, Inc. Rna templated ligation
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20220042090A1 (en) * 2018-09-14 2022-02-10 Cold Spring Harbor Laboratory PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL)
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN113631240B (zh) * 2019-02-14 2023-08-08 高丽大学校算学协力团 从生物样品中提取微泡的方法
JP7344973B2 (ja) * 2019-03-01 2023-09-14 マーシー バイオアナリティクス, インコーポレイテッド 標的実体を検出するためのシステム、組成物、及び方法
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN214973877U (zh) 2019-08-09 2021-12-03 胡桃钳医疗公司 用于形成治疗性多核苷酸的微流体设备及微流体路径装置
CA3153533A1 (en) * 2019-09-09 2021-03-18 Sherlock Biosciences, Inc. System
CA3154045A1 (en) * 2019-10-10 2021-04-15 Irvin Mark MODLIN Compositions, methods and kits for biological sample and rna stabilization
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CA3158080A1 (en) * 2019-11-26 2021-06-03 Huy Quoc NGUYEN Compositions, sets, and methods related to target analysis
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
ES2999535T3 (en) 2020-06-10 2025-02-26 10X Genomics Inc Methods for determining a location of an analyte in a biological sample
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
CN111944875A (zh) * 2020-06-28 2020-11-17 温州医科大学附属眼视光医院 一种防止rna降解的保护方法、保护序列、组合物和试剂盒及其应用
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
KR20230138683A (ko) * 2022-03-24 2023-10-05 전북대학교산학협력단 역전사를 사용하지 않는 결찰 방법을 이용한 cDNA 합성 기반 표적 유전자 검출용 조성물 및 다중 결찰 보조 재조합효소 중합효소 증폭 방법
EP4504966A1 (en) * 2022-04-08 2025-02-12 Illumina, Inc. Aptamer dynamic range compression and detection techniques
WO2024080776A1 (ko) * 2022-10-14 2024-04-18 고려대학교 산학협력단 비색 검출을 위한 황 함유 프로브 및 핵산 내 황 도입을 통한 효소 활성 가속화 방법
WO2025168224A1 (en) * 2024-02-08 2025-08-14 Leica Microsystems Cms Gmbh Marker and method for analysing a biological sample

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009536525A (ja) * 2006-05-10 2009-10-15 ディクステリティー ダイアグノーティクス 化学反応性オリゴヌクレオチドプローブを使用した核酸標的の検出
WO2010114599A1 (en) * 2009-04-01 2010-10-07 Dx Terity Diagnostics Inc. Chemical ligation dependent probe amplification (clpa)

Family Cites Families (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
DE68927373T2 (de) 1988-06-24 1997-03-20 Amgen Inc., Thousand Oaks, Calif. Verfahren und mittel zum nachweis von nukleinsäuresequenzen
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
EP0439182B1 (en) 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
IL99025A0 (en) 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
US5683874A (en) 1991-03-27 1997-11-04 Research Corporation Technologies, Inc. Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
AU684279B2 (en) 1993-04-12 1997-12-11 Northwestern University Method of forming oligonucleotides
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
US6077668A (en) 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
CA2105364A1 (en) 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US5808036A (en) 1993-09-01 1998-09-15 Research Corporation Technologies Inc. Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5591578A (en) 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
WO1996015271A1 (en) 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
US5674683A (en) 1995-03-21 1997-10-07 Research Corporation Technologies, Inc. Stem-loop and circular oligonucleotides and method of using
US5780613A (en) 1995-08-01 1998-07-14 Northwestern University Covalent lock for self-assembled oligonucleotide constructs
EP2368897B1 (en) 1996-02-09 2016-10-19 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6218108B1 (en) 1997-05-16 2001-04-17 Research Corporation Technologies, Inc. Nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor
EP0912761A4 (en) * 1996-05-29 2004-06-09 Cornell Res Foundation Inc DETERMINATION OF DIFFERENCES IN THE NUCLEIC ACID SEQUENCE BY MEANS OF A COMBINATION OF LIGASE DETERMINATION AND POLYMERASE CHAIN REACTION
WO1997046568A1 (en) 1996-06-07 1997-12-11 California Institute Of Technology Nucleic acid mediated electron transfer
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU739375B2 (en) 1996-11-05 2001-10-11 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
US5922553A (en) 1996-11-21 1999-07-13 Trustees Of The University Of Pennsylvania Method of detecting protein by immuno RNA
US7090804B2 (en) 1998-01-27 2006-08-15 Clinical Mirco Sensors, Inc. Amplification of nucleic acids with electronic detection
AU2898299A (en) 1998-03-04 1999-09-20 Onyx Pharmaceuticals, Inc. Baculovirus expression system and method for high throughput expression of genetic material
AU3671099A (en) 1998-04-29 1999-11-16 Trustees Of Boston University Methods and compositions pertaining to pd-loops
US7033753B1 (en) 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US6297016B1 (en) 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
US6479650B1 (en) 1999-12-14 2002-11-12 Research Corporation Technologies, Inc. Fluorescent nucleoside analogs and combinatorial fluorophore arrays comprising same
US20050214825A1 (en) * 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
EP1130113A1 (en) 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
AU2001265121A1 (en) 2000-05-30 2001-12-11 Applera Corporation Methods for detecting target nucleic acids using coupled ligation and amplification
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
US6511810B2 (en) 2000-07-03 2003-01-28 Applera Corporation Polynucleotide sequence assay
ATE426041T1 (de) * 2001-02-27 2009-04-15 Virco Bvba Zirkulare sonden amplifizierung (cpa) von zirkularisierten nucleinsaure-molekulen
WO2002083837A1 (en) 2001-04-11 2002-10-24 Ptc Therapeutics, Inc. Methods for identifying small molecules that bind specific rna structural motifs
FI20021325A0 (fi) 2002-07-05 2002-07-05 Valtion Teknillinen Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
JP2006510348A (ja) 2002-07-18 2006-03-30 ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ 蛍光消光脱離基を用いる化学ライゲーションの検出
US7153658B2 (en) 2002-09-19 2006-12-26 Applera Corporation Methods and compositions for detecting targets
EP1540003A4 (en) 2002-09-19 2006-06-21 Applera Corp METHOD AND COMPOSITION FOR DETECTING TARGETS
US20040259105A1 (en) 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
ES2442791T3 (es) * 2002-10-11 2014-02-13 Erasmus Universiteit Rotterdam Cebadores para la amplificación de ácidos nucleicos para estudios de clonalidad basados en PCR de transposiciones en BCL2-IGH
US20040235005A1 (en) 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US20040110134A1 (en) 2002-12-05 2004-06-10 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
KR100959101B1 (ko) 2003-02-20 2010-05-25 삼성전자주식회사 Pcr 반응기 및 pcr 반응기의 입구와 출구의 개폐를조절하는 방법
GB0304371D0 (en) 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
CA2520811C (en) * 2003-03-28 2013-05-28 Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities Method of cdna synthesis
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
EP1680517B1 (en) 2003-11-04 2010-03-31 Applied Biosystems, LLC Concatameric ligation products: compositions, methods and kits for same
WO2005071412A2 (en) 2004-01-09 2005-08-04 Applera Corporation Phosphor particle coded beads
US20050208503A1 (en) 2004-03-16 2005-09-22 Handy Yowanto Chemical ligation of nucleic acids
EP1727913B8 (en) 2004-03-24 2009-08-19 Applied Biosystems, LLC Ligation and amplification reactions for determining target molecules
WO2006076017A2 (en) 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7745614B2 (en) * 2004-09-03 2010-06-29 The Board Of Trustees Of The Leland Stanford Junior University Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
US20060223122A1 (en) * 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
US20060223066A1 (en) * 2005-03-29 2006-10-05 Applera Corporation Methods for normalizing and for identifying small nucleic acids
GB2424946A (en) * 2005-04-05 2006-10-11 Stratec Biomedical Systems Ag A detection system for substance binding using up-converting fluorescent probes
US7799530B2 (en) 2005-09-23 2010-09-21 Celera Corporation Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof
GB0808413D0 (en) * 2008-05-09 2008-06-18 Univ Nottingham Stabilisation of blood cell conjugates
WO2011091393A1 (en) * 2010-01-25 2011-07-28 Rd Biosciences, Inc. Self-folding amplification of target nucleic acid
US9506112B2 (en) * 2010-02-05 2016-11-29 Siemens Healthcare Diagnostics Inc. Increasing multiplex level by externalization of passive reference in polymerase chain reactions
ES2623859T3 (es) * 2010-03-04 2017-07-12 Miacom Diagnostics Gmbh FISH múltiple mejorada
US9609769B2 (en) 2010-07-29 2017-03-28 Rockwell Automation Technologies, Inc. System and method for ventilating and isolating electrical equipment
AU2012255121B2 (en) * 2011-05-17 2017-03-09 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
WO2012162161A1 (en) * 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method
US9901705B2 (en) 2013-03-15 2018-02-27 Armour Technologies, Inc. Medical device curving apparatus, system, and method of use
FR3054894A1 (fr) 2016-08-03 2018-02-09 Stmicroelectronics (Crolles 2) Sas Dispositif integre photonique a compacite amelioree

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009536525A (ja) * 2006-05-10 2009-10-15 ディクステリティー ダイアグノーティクス 化学反応性オリゴヌクレオチドプローブを使用した核酸標的の検出
WO2010114599A1 (en) * 2009-04-01 2010-10-07 Dx Terity Diagnostics Inc. Chemical ligation dependent probe amplification (clpa)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6015018492; J Clin Microbiol. 1996;34(3):501-507 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180039643A (ko) * 2015-07-17 2018-04-18 나노스트링 테크놀로지스, 인크. 절편화된 조직의 사용자-한정된 영역에서의 복수의 단백질의 동시적인 정량화
KR102545430B1 (ko) 2015-07-17 2023-06-19 나노스트링 테크놀로지스, 인크. 절편화된 조직의 사용자-한정된 영역에서의 복수의 단백질의 동시적인 정량화
US12435361B2 (en) 2015-07-17 2025-10-07 Bruker Spatial Biology, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
JP2022506752A (ja) * 2018-11-05 2022-01-17 サントル・アンリ・ベクレル がんを診断するための方法及び関連するキット
JP2023545218A (ja) * 2020-10-06 2023-10-26 マテリアルズ アンド マシーンズ コーポレーション オブ アメリカ 生物学的試料からのrna又はdnaの検出のための方法
JP2022145605A (ja) * 2021-03-18 2022-10-04 ゲノミル ヘルス オサケユイチア 希釈または非精製試料における核酸の正確な並行定量のための方法

Also Published As

Publication number Publication date
AU2012255121B2 (en) 2017-03-09
KR102085242B1 (ko) 2020-03-05
CN106434871A (zh) 2017-02-22
CN106434871B (zh) 2020-08-18
DK2710145T3 (en) 2016-02-22
US20180030520A1 (en) 2018-02-01
CN103827317B (zh) 2016-09-07
KR20140064735A (ko) 2014-05-28
US20140171338A1 (en) 2014-06-19
CN103827317A (zh) 2014-05-28
AU2012255121A1 (en) 2014-01-16
WO2012158967A1 (en) 2012-11-22
CA2836577C (en) 2019-06-25
US9745616B2 (en) 2017-08-29
US10066257B2 (en) 2018-09-04
EP2710145B1 (en) 2015-12-09
CA2836577A1 (en) 2012-11-22
EP2710145A1 (en) 2014-03-26
US20130005594A1 (en) 2013-01-03
HK1198548A1 (en) 2015-05-15

Similar Documents

Publication Publication Date Title
AU2012255121B2 (en) Methods and compositions for detecting target nucleic acids
EP2765205B1 (en) Chemical ligation dependent probe amplification (CLPA)
EP2677039B1 (en) Detection of nucleic acid targets using chemically reactive oligonucleotide probes
AU2015243130B2 (en) Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
HK1198548B (en) Methods and compositions for detecting target nucleic acids
HK1167269B (en) Chemical ligation dependent probe amplification (clpa)
HK1201081B (en) Chemical ligation dependent probe amplification (clpa)

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140319

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150401

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20150401

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20150401

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20150421

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150512

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150810

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150911

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151009

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20160215

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20160725